LOCUS       DADVXN010000001        77951 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.001, whole genome shotgun sequence.
ACCESSION   DADVXN010000001 DADVXN010000000
VERSION     DADVXN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 77951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.001"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..875)
                     /locus_tag="KB942_000001"
     CDS             complement(<1..875)
                     /locus_tag="KB942_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6850461.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTG"
     gene            1058..2182
                     /locus_tag="KB942_000002"
     CDS             1058..2182
                     /locus_tag="KB942_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6850462.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2409..3326
                     /locus_tag="KB942_000003"
     CDS             2409..3326
                     /locus_tag="KB942_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6850463.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3319..4152
                     /locus_tag="KB942_000004"
     CDS             3319..4152
                     /locus_tag="KB942_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6850464.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4235..5353
                     /locus_tag="KB942_000005"
     CDS             4235..5353
                     /locus_tag="KB942_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6850465.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5347..7245
                     /locus_tag="KB942_000006"
     CDS             5347..7245
                     /locus_tag="KB942_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBB6850466.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            7239..8462
                     /locus_tag="KB942_000007"
     CDS             7239..8462
                     /locus_tag="KB942_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6850467.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATMEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8548..10482)
                     /locus_tag="KB942_000008"
     CDS             complement(8548..10482)
                     /locus_tag="KB942_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6850468.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            10708..11364
                     /locus_tag="KB942_000009"
     CDS             10708..11364
                     /locus_tag="KB942_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6850469.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(11438..11791)
                     /locus_tag="KB942_000010"
     CDS             complement(11438..11791)
                     /locus_tag="KB942_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6850470.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11983..12912
                     /locus_tag="KB942_000011"
     CDS             11983..12912
                     /locus_tag="KB942_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB6850471.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12923..13759
                     /locus_tag="KB942_000012"
     CDS             12923..13759
                     /locus_tag="KB942_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBB6850472.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            14007..14783
                     /locus_tag="KB942_000013"
     CDS             14007..14783
                     /locus_tag="KB942_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6850473.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14959..15702
                     /locus_tag="KB942_000014"
     CDS             14959..15702
                     /locus_tag="KB942_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850474.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            15695..17275
                     /locus_tag="KB942_000015"
     CDS             15695..17275
                     /locus_tag="KB942_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBB6850475.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(17526..18005)
                     /locus_tag="KB942_000016"
     CDS             complement(17526..18005)
                     /locus_tag="KB942_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6850476.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18021..18773)
                     /locus_tag="KB942_000017"
     CDS             complement(18021..18773)
                     /locus_tag="KB942_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6850477.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18890..18960
                     /locus_tag="KB942_000018"
     tRNA            18890..18960
                     /locus_tag="KB942_000018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18922..18924,aa:Gly,seq:ccc)
     gene            19321..20046
                     /locus_tag="KB942_000019"
     CDS             19321..20046
                     /locus_tag="KB942_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBB6850478.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            20301..20567
                     /locus_tag="KB942_000020"
     CDS             20301..20567
                     /locus_tag="KB942_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBB6850479.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            20564..20995
                     /gene="ruvX"
                     /locus_tag="KB942_000021"
     CDS             20564..20995
                     /gene="ruvX"
                     /locus_tag="KB942_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBB6850480.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            21020..21325
                     /locus_tag="KB942_000022"
     CDS             21020..21325
                     /locus_tag="KB942_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBB6850481.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21406..22551)
                     /locus_tag="KB942_000023"
     CDS             complement(21406..22551)
                     /locus_tag="KB942_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6850482.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22612..23259)
                     /locus_tag="KB942_000024"
     CDS             complement(22612..23259)
                     /locus_tag="KB942_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6850483.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            23453..23746
                     /locus_tag="KB942_000025"
     CDS             23453..23746
                     /locus_tag="KB942_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850484.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            23790..24101
                     /locus_tag="KB942_000026"
     CDS             23790..24101
                     /locus_tag="KB942_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850485.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            24098..24722
                     /locus_tag="KB942_000027"
                     /pseudo
     CDS             24098..24722
                     /locus_tag="KB942_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24863..25021
                     /locus_tag="KB942_000028"
     CDS             24863..25021
                     /locus_tag="KB942_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850486.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            25022..25384
                     /locus_tag="KB942_000029"
     CDS             25022..25384
                     /locus_tag="KB942_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850487.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            25421..26269
                     /locus_tag="KB942_000030"
     CDS             25421..26269
                     /locus_tag="KB942_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBB6850488.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            26259..27734
                     /locus_tag="KB942_000031"
     CDS             26259..27734
                     /locus_tag="KB942_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBB6850489.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            28000..28407
                     /locus_tag="KB942_000032"
     CDS             28000..28407
                     /locus_tag="KB942_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBB6850490.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            28410..28625
                     /locus_tag="KB942_000033"
     CDS             28410..28625
                     /locus_tag="KB942_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850491.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            28629..29009
                     /locus_tag="KB942_000034"
     CDS             28629..29009
                     /locus_tag="KB942_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6850492.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            29143..29298
                     /locus_tag="KB942_000035"
     CDS             29143..29298
                     /locus_tag="KB942_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBB6850493.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            29367..29840
                     /locus_tag="KB942_000036"
     CDS             29367..29840
                     /locus_tag="KB942_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6850494.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            29837..31531
                     /locus_tag="KB942_000037"
     CDS             29837..31531
                     /locus_tag="KB942_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6850495.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            31497..31682
                     /locus_tag="KB942_000038"
     CDS             31497..31682
                     /locus_tag="KB942_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850496.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            31686..32861
                     /locus_tag="KB942_000039"
     CDS             31686..32861
                     /locus_tag="KB942_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6850497.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            32854..34377
                     /locus_tag="KB942_000040"
     CDS             32854..34377
                     /locus_tag="KB942_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6850498.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            34433..34717
                     /locus_tag="KB942_000041"
     CDS             34433..34717
                     /locus_tag="KB942_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6850499.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            34704..35039
                     /locus_tag="KB942_000042"
     CDS             34704..35039
                     /locus_tag="KB942_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6850500.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            35334..35960
                     /locus_tag="KB942_000043"
     CDS             35334..35960
                     /locus_tag="KB942_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850501.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            36702..37538
                     /locus_tag="KB942_000044"
     CDS             36702..37538
                     /locus_tag="KB942_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBB6850502.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            37602..38033
                     /locus_tag="KB942_000045"
     CDS             37602..38033
                     /locus_tag="KB942_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6850503.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(38095..39417)
                     /locus_tag="KB942_000046"
     CDS             complement(38095..39417)
                     /locus_tag="KB942_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBB6850504.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(39571..41211)
                     /locus_tag="KB942_000047"
     CDS             complement(39571..41211)
                     /locus_tag="KB942_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBB6850505.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            41544..42014
                     /locus_tag="KB942_000048"
     CDS             41544..42014
                     /locus_tag="KB942_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBB6850506.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            42236..43339
                     /locus_tag="KB942_000049"
     CDS             42236..43339
                     /locus_tag="KB942_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6850507.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            43623..44303
                     /locus_tag="KB942_000050"
     CDS             43623..44303
                     /locus_tag="KB942_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6850508.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            44393..45427
                     /locus_tag="KB942_000051"
     CDS             44393..45427
                     /locus_tag="KB942_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6850509.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            45441..45776
                     /locus_tag="KB942_000052"
     CDS             45441..45776
                     /locus_tag="KB942_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850510.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            45760..48240
                     /locus_tag="KB942_000053"
     CDS             45760..48240
                     /locus_tag="KB942_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850511.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            48296..49855
                     /locus_tag="KB942_000054"
     CDS             48296..49855
                     /locus_tag="KB942_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850512.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            49852..51216
                     /locus_tag="KB942_000055"
     CDS             49852..51216
                     /locus_tag="KB942_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6850513.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            51387..52457
                     /locus_tag="KB942_000056"
     CDS             51387..52457
                     /locus_tag="KB942_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850514.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            52472..53140
                     /locus_tag="KB942_000057"
     CDS             52472..53140
                     /locus_tag="KB942_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850515.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            53160..53738
                     /locus_tag="KB942_000058"
     CDS             53160..53738
                     /locus_tag="KB942_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6850516.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            54139..55050
                     /locus_tag="KB942_000059"
     CDS             54139..55050
                     /locus_tag="KB942_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6850517.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(55138..56055)
                     /locus_tag="KB942_000060"
     CDS             complement(55138..56055)
                     /locus_tag="KB942_000060"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBB6850518.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            56257..56694
                     /gene="zapA"
                     /locus_tag="KB942_000061"
     CDS             56257..56694
                     /gene="zapA"
                     /locus_tag="KB942_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBB6850519.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56753..57301
                     /locus_tag="KB942_000062"
     CDS             56753..57301
                     /locus_tag="KB942_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBB6850520.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            57416..59776
                     /locus_tag="KB942_000063"
     CDS             57416..59776
                     /locus_tag="KB942_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBB6850521.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59863..60177
                     /gene="trxA"
                     /locus_tag="KB942_000064"
     CDS             59863..60177
                     /gene="trxA"
                     /locus_tag="KB942_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBB6850522.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            60359..62137
                     /gene="uvrC"
                     /locus_tag="KB942_000065"
     CDS             60359..62137
                     /gene="uvrC"
                     /locus_tag="KB942_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBB6850523.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            62282..63181
                     /locus_tag="KB942_000066"
     CDS             62282..63181
                     /locus_tag="KB942_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850524.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            63174..64436
                     /locus_tag="KB942_000067"
     CDS             63174..64436
                     /locus_tag="KB942_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850525.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            64597..66495
                     /locus_tag="KB942_000068"
     CDS             64597..66495
                     /locus_tag="KB942_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBB6850526.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(66825..67544)
                     /locus_tag="KB942_000069"
     CDS             complement(66825..67544)
                     /locus_tag="KB942_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBB6850527.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            67881..68213
                     /locus_tag="KB942_000070"
     CDS             67881..68213
                     /locus_tag="KB942_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850528.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            68200..70203
                     /locus_tag="KB942_000071"
     CDS             68200..70203
                     /locus_tag="KB942_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBB6850529.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            70222..70692
                     /locus_tag="KB942_000072"
     CDS             70222..70692
                     /locus_tag="KB942_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBB6850530.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            70713..71297
                     /locus_tag="KB942_000073"
     CDS             70713..71297
                     /locus_tag="KB942_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBB6850531.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            71312..72298
                     /locus_tag="KB942_000074"
     CDS             71312..72298
                     /locus_tag="KB942_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBB6850532.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            72288..72599
                     /locus_tag="KB942_000075"
     CDS             72288..72599
                     /locus_tag="KB942_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBB6850533.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            72621..74402
                     /locus_tag="KB942_000076"
     CDS             72621..74402
                     /locus_tag="KB942_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBB6850534.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            74395..75771
                     /locus_tag="KB942_000077"
     CDS             74395..75771
                     /locus_tag="KB942_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBB6850535.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75778..76413
                     /locus_tag="KB942_000078"
     CDS             75778..76413
                     /locus_tag="KB942_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBB6850536.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            76443..77798
                     /locus_tag="KB942_000079"
     CDS             76443..77798
                     /locus_tag="KB942_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBB6850537.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        25829 a        13117 c        16153 g        22852 t
ORIGIN      
        1 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
       61 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
      121 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
      181 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
      241 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
      301 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
      361 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
      421 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
      481 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
      541 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
      601 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
      661 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
      721 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
      781 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
      841 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
      901 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
      961 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     1021 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     1081 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     1141 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     1201 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     1261 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     1321 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     1381 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     1441 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     1501 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     1561 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     1621 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     1681 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     1741 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     1801 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     1861 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     1921 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     1981 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgcaaa
     2041 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     2101 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     2161 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     2221 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     2281 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     2341 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     2401 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     2461 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     2521 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     2581 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     2641 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     2701 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     2761 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     2821 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     2881 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     2941 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     3001 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     3061 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     3121 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     3181 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     3241 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     3301 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     3361 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     3421 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     3481 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     3541 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     3601 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     3661 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     3721 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     3781 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     3841 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     3901 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     3961 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     4021 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     4081 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     4141 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     4201 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     4261 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     4321 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     4381 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     4441 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     4501 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     4561 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     4621 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     4681 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     4741 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     4801 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     4861 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     4921 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     4981 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     5041 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     5101 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     5161 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     5221 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     5281 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     5341 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     5401 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     5461 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     5521 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     5581 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     5641 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     5701 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     5761 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     5821 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     5881 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     5941 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     6001 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     6061 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     6121 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     6181 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     6241 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     6301 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     6361 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     6421 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     6481 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     6541 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     6601 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     6661 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     6721 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     6781 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     6841 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     6901 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     6961 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     7021 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     7081 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     7141 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     7201 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     7261 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     7321 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     7381 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     7441 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     7501 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     7561 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     7621 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
     7681 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
     7741 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
     7801 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
     7861 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
     7921 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
     7981 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
     8041 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
     8101 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
     8161 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
     8221 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
     8281 ggaaaattat gcgacaattg aaagaaagat atgctacaat ggaagaagaa gatggctttt
     8341 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
     8401 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
     8461 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
     8521 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
     8581 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
     8641 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
     8701 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
     8761 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
     8821 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
     8881 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
     8941 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
     9001 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
     9061 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
     9121 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
     9181 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
     9241 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
     9301 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
     9361 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
     9421 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
     9481 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
     9541 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
     9601 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
     9661 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
     9721 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
     9781 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
     9841 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
     9901 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
     9961 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    10021 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    10081 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    10141 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    10201 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    10261 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    10321 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    10381 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    10441 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    10501 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    10561 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    10621 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    10681 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    10741 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    10801 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    10861 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    10921 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    10981 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    11041 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    11101 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    11161 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    11221 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    11281 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    11341 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    11401 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    11461 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    11521 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    11581 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    11641 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    11701 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    11761 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    11821 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    11881 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    11941 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    12001 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    12061 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    12121 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    12181 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    12241 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    12301 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    12361 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    12421 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    12481 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    12541 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    12601 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    12661 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    12721 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    12781 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    12841 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    12901 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    12961 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    13021 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    13081 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    13141 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    13201 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    13261 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    13321 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    13381 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    13441 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    13501 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    13561 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    13621 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    13681 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    13741 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    13801 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    13861 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    13921 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    13981 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    14041 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    14101 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    14161 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    14221 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    14281 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    14341 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    14401 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    14461 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    14521 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    14581 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    14641 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    14701 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    14761 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    14821 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    14881 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    14941 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    15001 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    15061 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    15121 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    15181 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    15241 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    15301 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    15361 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    15421 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    15481 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    15541 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    15601 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    15661 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    15721 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    15781 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    15841 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    15901 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    15961 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    16021 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    16081 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    16141 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    16201 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    16261 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    16321 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    16381 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    16441 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    16501 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    16561 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    16621 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    16681 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    16741 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    16801 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    16861 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    16921 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    16981 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    17041 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    17101 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    17161 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    17221 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    17281 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    17341 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    17401 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    17461 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    17521 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    17581 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    17641 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    17701 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    17761 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    17821 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    17881 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    17941 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    18001 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    18061 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    18121 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    18181 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    18241 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    18301 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    18361 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    18421 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    18481 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    18541 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    18601 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    18661 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    18721 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    18781 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    18841 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    18901 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    18961 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    19021 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    19081 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    19141 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    19201 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    19261 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    19321 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    19381 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    19441 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    19501 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    19561 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    19621 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    19681 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    19741 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    19801 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    19861 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    19921 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    19981 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    20041 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    20101 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    20161 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    20221 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    20281 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    20341 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    20401 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccgtcctaca
    20461 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    20521 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    20581 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    20641 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    20701 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    20761 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    20821 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    20881 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    20941 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    21001 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    21061 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    21121 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    21181 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    21241 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    21301 attcatggcg gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg
    21361 tttctccttt ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa
    21421 gtgttgtact aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca
    21481 atcgatcgtc tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc
    21541 tccagcgtcc aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg
    21601 aaccatagct ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag
    21661 ttctttatag ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata
    21721 tttccgctga ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag
    21781 aaccccagca ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct
    21841 tagtgtttta ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc
    21901 aagggcttca ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa
    21961 gtcgtttcga taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc
    22021 tttactgtaa actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac
    22081 gttgtctagt ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt
    22141 agaagttgaa tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg
    22201 ttgggctttt tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca
    22261 ctttctaaac ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt
    22321 ttctagccac aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt
    22381 tttatcgtag tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta
    22441 acctcgtcta cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc
    22501 atagagccaa aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt
    22561 ccctccatag tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga
    22621 atagtgttta tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata
    22681 aaattcttga tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc
    22741 atatcgtaaa agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg
    22801 aaattcaatt cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg
    22861 tgagacagtc aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat
    22921 tatagagtta tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat
    22981 ttcgggcatc atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga
    23041 tacatctaat acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt
    23101 ttcccaatta ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc
    23161 cattgagagt cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc
    23221 ggggtatttt tttctagcca cattttcacc accattcata tattttctct actatatcac
    23281 gatttaaata tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga
    23341 aaaggtatga tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg
    23401 cattttttta aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt
    23461 aaaaattgac aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt
    23521 tcaacaagta gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt
    23581 tccaaaatat atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat
    23641 gatgcagaaa tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat
    23701 ttcacaagat gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg
    23761 ggcaaaggga ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa
    23821 aagtattatc aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca
    23881 aagcgctaga agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac
    23941 cacaagaacc aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt
    24001 ttaaagaacg actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt
    24061 tagcgagctt gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg
    24121 acacattata agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc
    24181 ggaagcgtta ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt
    24241 tactactagt aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga
    24301 aaaaaaatat ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag
    24361 actggatttt cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag
    24421 tattccgcat gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta
    24481 ttactgaacc aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat
    24541 caactattta tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg
    24601 ttgatcgtga acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg
    24661 ggttgcagtt ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt
    24721 aggagcatta acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac
    24781 aggataaagg caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat
    24841 taaaaaatcg gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg
    24901 cggaattttc ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt
    24961 atatctcttc actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta
    25021 gatgtacaaa acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca
    25081 agggatcgct gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga
    25141 aaaactaaca gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga
    25201 aatcaacgag ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga
    25261 agcaagggtg aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga
    25321 atttgttcgg gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga
    25381 atgatttata ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc
    25441 agcaacggaa ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat
    25501 tccaataaaa ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga
    25561 atggtggacg gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt
    25621 ggtagttgac ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact
    25681 tgcttcaatg tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat
    25741 ccatttcttc tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc
    25801 tgatatcttc gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa
    25861 aggagttcca atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc
    25921 tattgaaaac gtatctctaa ataatattgc accagctccc gattggctag tgaacctact
    25981 gctaaaaaaa ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc
    26041 aaaaaaatta gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac
    26101 taagttatgc ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat
    26161 ttatacagca aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat
    26221 tcgatcgatg attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag
    26281 atagtatttt agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa
    26341 cctggatgtc acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga
    26401 atgcagaatt gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat
    26461 ttagcggata cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat
    26521 gggaagacag ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt
    26581 ttgatgacaa caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc
    26641 cagtaaaaga acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt
    26701 ttattgacct tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga
    26761 tagtcggatc agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt
    26821 tggatggacc gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt
    26881 ttttcaccga ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag
    26941 gcaatgtaat tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga
    27001 taaaaaactt catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca
    27061 tagcttgggc aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag
    27121 atgatacagg aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc
    27181 cgtttaaaac aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg
    27241 ataaaggaca acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa
    27301 aagactatca agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt
    27361 tggaaatgga aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt
    27421 atcagaacta tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag
    27481 ctactatgca acaagagttt tatttagttg atagcgtact caccgctgat attttggaag
    27541 ctgtttttga taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa
    27601 agattgcctt gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa
    27661 ataaacgacg aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa
    27721 agtacaaaaa gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc
    27781 ttactgcccc aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact
    27841 tttgaaatag ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt
    27901 taccaaaacg ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg
    27961 taggtgttac cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca
    28021 aacaaagcac cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg
    28081 gcatttagtg tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag
    28141 ataatggaag aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta
    28201 gtgggatcat tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt
    28261 aattgtctgc ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat
    28321 aatttgtttt taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg
    28381 ataactaatt tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg
    28441 gtgagaaaca cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg
    28501 aagataaaat cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt
    28561 atattgaata caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt
    28621 ggtaatctat ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt
    28681 ataggcagaa gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa
    28741 gaaagcaaag cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt
    28801 catacttgta tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa
    28861 ggaaagctga tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag
    28921 atgaaactaa ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa
    28981 gacaaagacg agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac
    29041 aatcgatgcc tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac
    29101 aaataacgat tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta
    29161 ttgaaaaata caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct
    29221 cattttggtc aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta
    29281 gataaagaaa aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt
    29341 tttcttttgt ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag
    29401 gcaatatatc aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc
    29461 agcaaccatt aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat
    29521 ggaatcgtat cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt
    29581 cattggtagc ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa
    29641 aatcaggtac gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg
    29701 ttcaatcaca agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag
    29761 aatcacgatc aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca
    29821 aagagttgat gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc
    29881 acccaagaaa attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc
    29941 agatagcttc ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga
    30001 gttgctacca tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct
    30061 aggcagtctt tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt
    30121 taccagtatg agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc
    30181 gttgattatg gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt
    30241 gaaacaagct aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc
    30301 tagtaagttt ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc
    30361 taattcgttc gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac
    30421 aaccgcaatt cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa
    30481 gtcaggaatg acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga
    30541 tttaaacgtt cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga
    30601 acaagccgac aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga
    30661 tcaagagaaa tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat
    30721 gattccaacc attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt
    30781 attggtgaaa aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga
    30841 agactggcag gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg
    30901 agtggattta tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa
    30961 cggcgaactt tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa
    31021 agaaaagcgc gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac
    31081 tcaattagaa agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat
    31141 tcaagaaaat gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct
    31201 tatcagtaaa gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac
    31261 tctaaacgtt ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa
    31321 taaaaatacg attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg
    31381 cattcagatt aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa
    31441 tgcctatgtg tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga
    31501 attttataca agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg
    31561 gattggtgtg tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag
    31621 cgctgggcat agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt
    31681 aaaagatggc attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg
    31741 tggtatcaat ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa
    31801 atagtgatgt gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc
    31861 aattggtcaa aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga
    31921 aacctaattc agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc
    31981 taaacggtaa tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact
    32041 tattacctaa ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga
    32101 ttggtgataa aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc
    32161 aagatggttt aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac
    32221 aagctggcaa ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt
    32281 tgaaggttca taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg
    32341 aagaagccaa cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa
    32401 caatggacta caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg
    32461 attggaacac aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg
    32521 aaaatgaaca ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc
    32581 attactttaa tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta
    32641 attccgatca gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg
    32701 tcaaagggtc acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg
    32761 atggtggcga tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat
    32821 atcaattaaa agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa
    32881 cggctgacag aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa
    32941 aaacaaccaa cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca
    33001 aaagatttag gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta
    33061 tcaaatgtgt tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca
    33121 ggtacgttga aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac
    33181 acgacctatt caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc
    33241 tttgggttcg tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga
    33301 tcaatagata aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat
    33361 tcatcaaatg tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca
    33421 aaacaaacaa acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg
    33481 gaaaaaactt taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt
    33541 tcacaaggcg aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa
    33601 gaagtaatcg gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca
    33661 acagtaaaaa cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca
    33721 gtgttagcaa caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact
    33781 tcagttgatt ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg
    33841 gaagattcag cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa
    33901 aacactgaca ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct
    33961 acgttggacg atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg
    34021 gtaattctaa accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt
    34081 tacattttac aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta
    34141 gtattgattg cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg
    34201 ggggatattg cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa
    34261 aaattcgatt actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt
    34321 gatcaaaatg cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa
    34381 aatattgggc ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat
    34441 tttggatagc ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca
    34501 acaattgatt gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga
    34561 taaagatatg gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg
    34621 gtatttgaac cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt
    34681 acaacaaatg agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga
    34741 gagtcaagtt taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg
    34801 aagaaactgt atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg
    34861 ctagtgtagg taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt
    34921 atgaactaac aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa
    34981 tcaatgaagg aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga
    35041 atgattacac ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt
    35101 agctgcctga attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta
    35161 ggtgtggttg caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa
    35221 tacaggcatg ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga
    35281 acacgtctgt tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca
    35341 aagcagagtt tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta
    35401 ataccacaga ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc
    35461 aaaaactact actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat
    35521 ttgatagcag tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat
    35581 taatgtgttt ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac
    35641 cttttggaga tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa
    35701 aacatggtcg ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag
    35761 caagtctata tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag
    35821 actttataag tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga
    35881 tgcaaaaagt gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta
    35941 aagaacaaga agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg
    36001 tatgccattt ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc
    36061 ataaacgctg ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga
    36121 agaagaagaa tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa
    36181 cgtcttgtca caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt
    36241 cgctcgaaaa acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg
    36301 tcaaaacgtt gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    36361 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    36421 ggagctgaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    36481 ggtaataagt tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt
    36541 acttgatgca ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa
    36601 agtagaaaag ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta
    36661 acaatcgcta aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt
    36721 ttgtggtgat cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt
    36781 tgatcaaacc gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact
    36841 caacagttgc ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga
    36901 aatcactgaa ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag
    36961 tcggcaacat tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc
    37021 tgatccaaat ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc
    37081 atcagccaaa atttcatttg acgtcatgga cttggtacct tatatgtggg ggggttctgg
    37141 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    37201 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    37261 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    37321 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    37381 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    37441 ttttgatcaa tctttacaat gctttcgtca tcttacaaac attaggagaa tctagactaa
    37501 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaggagtat actgtgataa
    37561 aaaaattcat ttaaaaaaat aaaaattggc tggtgatttg gatgttgcgg gaaatgcgtt
    37621 taggagatgc agaagcacta cgagaaatca atgcggaaca attaggatat gatgtaccat
    37681 taaccgttac ggtgagacaa atgaagaaac tgttgaagga gccagaaaaa aattttttct
    37741 tggtctatga agatcctact tctaaaaaag cagctggcta tgtacaagcg gagtcctatg
    37801 aatcgatttt cagcgatccg atgttcaata ttatggcact tgctgtgtct aaacatgctg
    37861 aaaaaagagg aatcggaaag gccttgatga ctggcgtaga agaagcgaaa agacgaggaa
    37921 tatcagcaat aagacttaat tctgcagaat acagagtaga agcacaccga ttttatgaac
    37981 gcatcggtta tcattccgat aaaatgcaaa aaagattttt gaaaatcctg taaattttta
    38041 tcaaaaaata cctggtcatc aagccgatac ttgatgatca ggtatttttt ttgactaaga
    38101 aagtaattgc atcaagaaac ctgagatgat caaaacgatg gcaccaccaa gccgattgcc
    38161 catttgagca aaagcaataa gttccatccg attagctgca gataagacag ctacatttcc
    38221 tgtaccaccc atcgagttgt tgcataagcc agcagtaata gttgattcga ttggatataa
    38281 accaaacaaa cgaccgacaa atcctgaaac tactgaaatc gcgataacac ttgtcaaaca
    38341 taagacaaca aattgccatg tcaaagcaga agccaataca tttagattca ataacgcaat
    38401 accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttgt tgaacatgat
    38461 agctgcatct tcgtaatatt ctggtaatag gtttgcgatt ttacagataa caacgataat
    38521 gatcatgaat gcgtaagcat ggactttagg aacaaaatag ttacagattt gtcctaagat
    38581 gaagaaagtc aatgatacca tcaaaccgac acctaattgt ggaatggaag gttttacatt
    38641 tctaggtttt ccatcgccaa ggtcgccttt ttccattaat ttaccattac cgttgaattt
    38701 tggcattgat tgtcccgcac gagcaactaa cgctgcacca atgatagcta ggacatttgt
    38761 cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcgtccgtac ctaaaacatt
    38821 tgcataaatc gttgataaag gtacagctcc ggctccaaca ccaccagcca ttgcgggcaa
    38881 tgagatatat aaaacggaat ttgcaaaacc atttccgata agcataccta cgagtcctac
    38941 ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa cagaagcttt
    39001 catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag caatataaaa
    39061 gtctaaaaag cccatattgc tgacaaagtt ttctgtggat tttactacat actcaggtaa
    39121 gatcccaaat gtagcaatcg cggcagaggc aaagatcgca aaaacggagc caccacctaa
    39181 ataacttttg acgataggga tttttgttcc gataaaatga aataaattac cgaaaatgac
    39241 caaaacagcg attgggccaa tcatattcgt cggtaatttt cctaaagcca ttgcaatgat
    39301 caggacaagg accatgatca aataaagggg caaacttacg ccggtaattt ttgttgacca
    39361 aaacccttct tttttctttt cctcaactgc agttgcggtt gttgtttttt gtgccatttt
    39421 tacagatcct ttcttattct gtatcgtatg aaaaaagcca aagagaagcg gcaaaagagc
    39481 agtagttggc tggctgctgt tttgccgcag tcgctttttt ctttttagac gtcatacaaa
    39541 gtttctgtat gttcttttac tataacaatc ttaatcttct aaagatttat attcaggaac
    39601 ccatttagca gattcaacag cttctttgat atccgtgatt tcttcacgat tcaatttttg
    39661 tgcaacaact gattttgcga cagtttcagc aatcgtttga gagaattcag tgatttttgc
    39721 tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc ctaatgctcg
    39781 actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa tcaaaccaag
    39841 ccctaaacct gggtacatca acgcattatt tgcttgaccg atttggtaag ttacaccgtt
    39901 atattccaca tcagcagcag ggatacctgt tccaactaaa gcttttccgt ccgtccattc
    39961 aatcaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag gcatgatgat
    40021 tggacgtggg gtatgagccg ccatttcttt cacgatttct tcagtgaatg ctccaggttg
    40081 ggtagaagta ccgatcatga tcgttggatg gattgctttt acaactgctt ctaagtttgt
    40141 caattcatca gcattagtga attctgagcg tttacgagcg aatgggattt gaccaggagt
    40201 caatcctttt gtatcttcaa acaataaacc ctgtttatct acttgataga aatgttggcg
    40261 tgcttcttct tcaggtacac cttgacggat catttcatct aaaaggatgt ttgcgatacc
    40321 tacaccagca gtaccagcac caaatgtcaa gatcgtttga tctttcaatt gctcacctga
    40381 tatattcaat ccacctaata caccagctag tacaacgatt cctgtgcctt ggatatcatc
    40441 attgaaagta gtgattttat cttcgtattt ttctaagatc gttgcagcat ttcctcgtcc
    40501 aaaatcttcc caatgtaata acaattctgg gaataattca gtagctgatt gaacgaattg
    40561 atcaatgaat tcgtagtaag tttcgccttc tacacgagca tgacgattcc ctaagtataa
    40621 tggattattc aataattctt cgttattagt accagcgtcg attgatacag gcaagacttg
    40681 tgcaggattg ataccagccg cagcagtgta gaccatcaat ttaccgatag cgatgtcaac
    40741 accgttcaca ccccagtcac ccatacctaa gattccttct gcatctgtca caacgatcaa
    40801 acgaatgtcg cgtccatctg ctgcgttttt caaacttgct ttgatatttt ctggttcgtc
    40861 gattgaaaga aatgcagcat cttgtggttt caagaaaatt tcgttgtatt gttcgattga
    40921 atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt cgcccatcaa
    40981 tttataaaac aatgtccggt tcgtattgaa tagattcatt aagaagatac gtttttccaa
    41041 atcggtctgt ttgcttaagt attgaccgta agcttggaca gattgttgtt ccaatgtttg
    41101 gacagtactt ggaagcatac cagtgattcc gtattttgcc cgttcttctt ttgtaaaagc
    41161 tgttccttta tttagaaaag gattatttaa taagtttagc cctgtaagca tgaatgattc
    41221 ctcctaagtt ttaatacaaa cgaagtatag gagtcagatg aggatatagt caaaaagagc
    41281 aggtataata aattttattt atatgagtaa tagagtcaaa agaaatcaaa atagcggtat
    41341 ttgttctttt tatatcaaca atccttgctt gattactatg cgtatctctt atatgctaaa
    41401 gaaagaaaag tagaagaata aaggatatgt tagatagata aatagaatct ctgaagaata
    41461 aatcacgtga tcgtgacgat aaaatacact tacgtcaggt gataaaaaat acataagtag
    41521 aaaactaaga ggaggaacag aaaatgaaag aagttcaatt gatcagaaaa agcgaactat
    41581 cagaaggtgg ttgcaatgca tgcggcgtag tagaagcaac aagctataca ttaaagcttg
    41641 gtgccaacaa agcgataatc tctgagttga cagtaggcgg tcttgtggac agtctggcgc
    41701 ttgctgaagg ttttattgga gaagatattt atgagatgtt ttcagaaatc cgtcagttaa
    41761 aaaaagggga aaactgcata gaggttcatc atgagagccc gaatgtgaga ttcaaacgtg
    41821 gtgataatga gatgatcttc aataatcatg tgtccgatca caccgagcta tatggaatcg
    41881 tgaatcaaat actaactgag ctttttggat tgggacctta tgcgttcaag gaagaaaacg
    41941 gtaatccaaa attaaacgaa gaatggcaag aaacgattga aatacaaaga aacaacccgc
    42001 acttatttca ataggcatcc acagtatttc gtaaaaggga ctttgacacg acttttgtca
    42061 aagtcccatc gttttttatc tatttgtgtt ctaaacattt ctttcagagt agtaacaact
    42121 ggaatgactg aaatgtaaac agattatttg cctgtatcaa tacggaagag taatataaag
    42181 tgtgtaaacg ctatcatatt attcattcaa ttttttatag agggaaggca ttgaaatgga
    42241 aaaaacgtat caagcaggta caaatgaagg aatagtggat tttatcaata tggaagattt
    42301 agaaatagcc gcttcacaag tgattccagc tggtggttat ggttacatca gcagtggtgc
    42361 tggcgactta tttacttatc aagaaaatga acgggcattc aatcatcagc tgattattcc
    42421 tcatgtatta aaagatgtag aattacccga tacaactaca cattttgaca aagaaacgct
    42481 gactgcacca atcatcatgg caccagttgc tgctcatggc ttagctcatg tgaaggcgga
    42541 gaaagcttct gcaaaaggtg tagcagattt tgggacgatc tatacagcaa gttcttacgc
    42601 ttcttgtaca ttagaagaga taagagaagc aggcggggag aaagcaccgc aatggtttca
    42661 attttatatg agcaaagatg atgggatcaa tttggatatt cttgaagtgg ccaagcgtaa
    42721 tggagcaaaa gcaatcgtat tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga
    42781 ccgcagaaat gggtttacct tcccactgcc aatgccgatc gttcaggctt atcaatcagg
    42841 agtaggccaa acgatggatg ctgtgtacaa atcttccaaa cagaagctta gtccaaaaga
    42901 tgtagaattt attgctgctc attctgattt gccagtctat gtgaaaggtg tccagtcaga
    42961 agaagatgtc tatcgttcat tagaatctgg tgctggcgga atctgggtat ctaatcatgg
    43021 tggccgtcaa ctagatggtg gtccagcagc gtttgattcg ttgcaatatg tggcagaagc
    43081 agtagataaa cgtgtaccaa tcgtgtttga cagcggtgta cgacgcggac agcatgtttt
    43141 caaagcaatc gcatcaggtg cagatcttgt tgctattggc agacccgtta tttatggatt
    43201 gtctttagga ggaagtacag gagtccatca agtgtttgat ttctttaaaa cagaattgga
    43261 aatggtcatg caattagctg gaacacaaac agtagaagat atcaaaaaaa taaaattgcg
    43321 tgagaatcgt tttatttaag aaacaaatga cgaaaaaaat aggagtgcgg cagatgatgc
    43381 cgtactccta tttttatagt tcaaggatgt cactaataga ataatttgaa acgaaaaaat
    43441 aaaattgaaa tattttttca tacttaataa aaaaggaaga atattcatgt aaacgatttt
    43501 attgtttttt tctcttttat acaaaaaggg tcataatttc aagtaagcgg atgaattcat
    43561 gaggattttt tgcatactgt ctctactata cttaggtcac taagaaaaag gagtgattta
    43621 ttatgaaaaa gaacatgatt gggacattat tactttcaac acttcttcta ggcggcttgg
    43681 caacaccagc ttttgctgag ggtcaagcga cttcaaaagg agatattacc tttactgaac
    43741 caacaaatac cgtggagcct cttaacccta ctgatccttc aaaaccagta gagccagcag
    43801 atccggaaaa tccagcaact ggccagacag gtcctttaac actggatgta gttcccgaat
    43861 tgccatttgg tacacacgag attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa
    43921 aaaacgatac accttatctc caagtatcag atcgtcgagg tgtaggtgca gatggccaag
    43981 cacaagggtg gaacgtgaca gtttcggttt cagattttgt aaacggttca caagttttgc
    44041 aaggcgctga attagatttt ggaacatcca ctgttaaaag tacgtcagat aacgaatcca
    44101 ctggaccaac tagtcaaact gtaactgggt tgagtaaagc aagtgcagcg acaccaatat
    44161 ttacagcagc aaaagatcaa ggtctgggaa catggctttc tgtttatgat ccagcaaata
    44221 tcacactgaa agtgccgaaa gcagcagctg gaacattcac ggctgatttg acttggaacc
    44281 tcgtggctgg cccagtagca tagagtatga aataaattca acaaactgcg gtgaaaatac
    44341 tataggtgaa ggatttttta ccgcagtttt tgttgaaagg atgtggtgga caatgaatca
    44401 aagtaaacga ctgtttgttt cgtttttttc tattttaagt ctatttttca tttttccgtc
    44461 aatcagccaa gcaaaggatt cagcaggaga ctttggaatc aaaccggtat ttccagagaa
    44521 ccaaatagat aaagcaatcg gttattttga tttattagtg gcaccggaac aaaatcagac
    44581 actagaagta attatcagca attcttcaga tgaagaacga acatttgagg tctctgtcaa
    44641 tccggcggta acgagtgatg gtggaacgat cgattatagt caaaaaaacc caacgttaga
    44701 tgagacattg ccgtttgatg tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt
    44761 atcagcacat gcagaaacaa ctgtacctat agaagtgaaa atcccagcaa aaagctttaa
    44821 gggacgtgta ttagcgggta ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc
    44881 tacagaagga gcacaaatca aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga
    44941 aagtcaagag acgatagaac cggatctgaa acttttatca ggagatttgg acgaggtcaa
    45001 cgcaaagcca actgttcagc ttcgtttcca aaatcctcag cctaggatta tcagcaattt
    45061 gatatttacg agcaagatct tttatgaaaa tcagctttat attgaaaata cgtctaatgc
    45121 atttttagtt gcaccaaatt cgaattttca cttgaatctt gatttagctg gtgataaagc
    45181 aaaagccggc gattaccgcg cagaaatcat agcaaaatca ggcgacagta atgaatggcg
    45241 atttacacaa aactttacga tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt
    45301 ttttgcagta caagaacaat ctttcccatg gctttatgca ggattggttg ctgcagttct
    45361 agtgttatgg ttgattttgc tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac
    45421 aacatgatga ggtgatttcg ttgaaaacat cgatcttatt gggagggatt attgcaagtt
    45481 tgctgttctt cagctggcaa gagccagtcc aagcagatac caaaagttca ggtacgatta
    45541 cattttatga tctaagtcca attccgcatc caagtggttc agtcaatggc gcatcaaaac
    45601 agcagacaca gctaccgatt tataaagaat ctaatcaaca tggcgtattc ccggagacga
    45661 atgaacaaaa gatccgtttt ctttatttga tcattggtat agaattatgg cttcttgcgt
    45721 ggttgttgtt atggagaaga aaggaaaagg aggatgaaca tgttgctaca aaataaattt
    45781 tcattttttc gctccttctt ttttacttcg ctttttgttt taagtatatt tatcttttca
    45841 ccttcggcag aagctgttgg agcaacgcct cctgccattt cttatgatcc atacacgtat
    45901 gaaaatatcg tacctccaaa tggtggacgt agtgcagtca atggcgggaa gcttgtccaa
    45961 ctaactgggg ctaaccaatt gcctaaattg actgctactt atttgaacga tggttcaaat
    46021 gatccttgga aaggtggcac ggtccgtttt cattgggaaa gccatgtgaa actaacaaat
    46081 gtacttgatt tggtgaatat cactgcacaa ggatttgcat ttgtggcgat catcaatttg
    46141 ccggaacatg tatcaagcag tgatgtatta aaagcaattg actggaacaa cgcctacttg
    46201 tatattgccg gtgcccccat caaggttact agcggaagtt tattatcagc aggaagccat
    46261 accttgcgtt ttgggatggg gtcgtgggat acaaatactc tattgacaga tattattgct
    46321 attattacgc gaggcgaatt caacgtggac gatatcccgc ttcaatttga tgttacagct
    46381 aatgtcgcga agatgacaga aaacggagat aaatatgata cgagtccaaa caaaattttg
    46441 accaagggta aattacaacc ttctatgtct aaaaaggccg ttttttctgt tgatttttat
    46501 gattcgaata atattatcca agatcgtatg ttcaattcga ttttagctcc agctgcaggt
    46561 agattttatc ctaaattaac tggcggaaac ttgaatccag gagcatatgc attttctgca
    46621 agtgcagaga taaatacgtg gaatagttac atctcaccaa gtgattcaac aggaaaatat
    46681 aatacatttg ctgataccac agaatacgtg gatggaaata accagaacct tccaggctct
    46741 accaatagtc ggacattgac gatggggctg actgaaggac gttttgatca gttgccattt
    46801 agtcgctttg atcgcgtagt caactatttt acaggaaaaa acgttacaca aggagcaata
    46861 ctgactcata ctccagcaag cacaagtgga ttgaatcaga cagccaatat cgtgtacagt
    46921 gggaaagatg cgcaaggcgg attgctttct cctgttgctt taaaggtatt ggagcagtac
    46981 aagctagacg gaacggtttt accaacaaac ttaacagccg atagtcagtg gggaaagctc
    47041 ctttcttctg catcgtacca agtcacggcc aattggtcag caactgaact aaaaaagggt
    47101 tcgatccatt acaggcttta ttcgaaagga acccaaaaat tagcgacagg atggacagat
    47161 caacttttcc aaacgatcca atcaaataac gggggtgtaa atacagctac ttctacacta
    47221 cctgcattgg cttcagggca atattatttt gattatcgct tggtggacga tgtacttact
    47281 aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac aagctggaat caccactctt
    47341 cctcaaataa cagtggctaa ttttccatct gtgagtgcag atagtcagct aaaaaacctt
    47401 agcagacaac ctgagacagg agatcccctg tctgctctat caggagatac gattcaagaa
    47461 aatgtgacat tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc
    47521 tcacttcctg aaaatactac ttatatcaat ggttccttaa aattaaatgg tacaactatc
    47581 gcagataccg gcattcaaca aggagtttct gtcccagcag atctattgag caaaatagga
    47641 gataccattc atcttactta caactatcaa ttaaatactg tagatacttc tttacaatcc
    47701 gtcagcatac tgacaaaggc agctgtactt tccagcaata ttacattagc agatggtgct
    47761 aagcttccta acccagttgt ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa
    47821 ttaacattag ttaatgtacc agatgatttt acatttggta acgatctccc caaaccgttg
    47881 aagacaagtt attatgaatc aaaaggcgat ttttcttttg atgtgcggga tactcggctt
    47941 ccaagcacgt ctccttggca actgacaggt acattgactt ctttatttaa gaatgatcaa
    48001 ggtcaagaat taagcggaac aaaattatac ttcaatcatt ctggatcaaa gcaattgatt
    48061 cagcaaggtc aaaacacgtt gatttatgag agtgatggaa cagctaaagg agaggttctc
    48121 gttgactttc ccgataccga tggtttactc ttagaggtaa atagcagtac gaatgctcaa
    48181 cctggcgcaa cgtatcaagg catggtcaca tggaaactga cagctgggcc aaccagctaa
    48241 gtgattagaa tttgtatgga tctgtgataa ctgtcacaga tcctcttttt caaggatgag
    48301 aaggaaactt ctgctgttaa aatcagaaat gcttgaagga gtggacttgg aacttttcag
    48361 tttgcttgaa aaaaatgaac aacggcaaat caaacttttc caacttttat tagaggaaaa
    48421 aggagaggtt tccgttaata acgtacttac ccgcttatca gttgatcggg ctaccttgaa
    48481 agaagatctg ttttatttac aagctaactt ggaaaatatc aaacaattgg aaattgttat
    48541 ccaagaaggg aatattttat taatcagaca tggaaatata gcagcagtcg atgtttatta
    48601 tcattgttac ttgcgctatt ctacaaaata tcagatactt ctttatctgt tggaaaatgg
    48661 ctcatttgat cggcagaaac ttgtcatgga acttaatatc agttcggcta ctttgtctcg
    48721 cagagtgaag gaattaaatg attctttaaa tgagtttcat ctgaaaataa aaagcgggcg
    48781 gctgaccggt ccagaaagtc aaattcgtta tttttatttc caattatttt ggtttggacg
    48841 tccctatctt gaaaacaaaa aagaatacag tgatttatca ggaaacactt ttttagatct
    48901 gctgcaaaaa gagtttagtt ttgctattac agaagaagga cgattgaaat ttgaaatatg
    48961 gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa
    49021 tcttcctgtt ggattgtttc aaacgacaca atttttattg gacctccata catttttgac
    49081 ccgttacttc tttcaatatg cctttccttg gaatcaaaat gaaacgttgg ttttttatct
    49141 tttcatgatc agtaacttta cattagatac acataactcg catgtccagg agtttttgat
    49201 aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat cgaacgttta aacaatcgat
    49261 agaagaatat ttctcttttt atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac
    49321 agtgatgcaa aatcacgctc attttgccta ttttagagga tggatgaata tttttggcca
    49381 agaaggatta aaacagcggt taactgaagt agaagataaa caattcctgg ttttatgtga
    49441 aagattggtc agacaaacat ttgctattgt tgattttact gaagtagacg aggaaaatgc
    49501 ccgctttgaa ttatttgctc gctatgcatc tattttacgg acgctgatca agcagttaga
    49561 attacgtctg caaattgctt atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa
    49621 agaaatcatc caggaacaat tgctgaatac gatcaaatat gatttagtcg aatacgagaa
    49681 agaaaagcag tatgatatca ttttgactag ccaattgaaa gagtacccgt cacagaagtc
    49741 cgctaaagtt tttgtattca ctagcaacga atataattat gatttcccct atttgaacca
    49801 atttttaaaa gaatgttatt tagaaaaatt gaatttaagg atgaataaaa catgaaaatc
    49861 gtgatcattg gtgcttccca tgcaggcatc acagctgccc taaatctaag aaaacttcaa
    49921 ccgaaagcag aagttttact gatcgatgaa gatcataaag atggactagg ctatgtttca
    49981 aatggaatca acctttattt aaaaggaaag atacgtagtt tagctgaagt tgcccataac
    50041 atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt aactgaactt
    50101 gatccagaca aacatcagct catcctcgca tccaaagaag gaaaaaacga gaccataacc
    50161 tatgataaat taatcgtagc gacaggttcc tctcctgtca ccttgtataa acaaattgaa
    50221 gctgaaaatg tctacaccta taaaaatcta gtgcaatcta agcaagtact tgccgcttta
    50281 aaagaagcaa aagaagtagt gatcttcggt gctggttaca ttggattgga attagcagat
    50341 gctttgagaa ataaaggata cattattcat ctagttgatt atatgccgaa tgttttatca
    50401 agatatttcg ataaggatat gatcaacagt tttcaaaatc agctgcaaac taagcaaata
    50461 aatttttatc caaatgaatt cttgatcgat tggaaaaaat cagaagagaa agtagtatcc
    50521 gtacaacttc tgtctcaaac aataaaggca gatatggtca ttttttctgc ccaaactcgt
    50581 cctaatacga cattgttaaa agaaaaagtg gctttgtatg aggatgagac tgtgatagtc
    50641 aatgaatatc tgcagacaag tgatccggac atctatgcga ttggagatat cgtcccagtc
    50701 agttttgaca aaaataaacg gcatctcttt ttacctttag ttactcgagc tgtacatatg
    50761 gcaagagcag tagcgctaac tttgagtggt cagccaaccg cttacgacct cagacaaaag
    50821 ataacagcag cagtcattat agactacttt ttaggaactg tagggctgac agaagatgaa
    50881 gcgccattct tggaacaaaa taccggttcg tgttcaggag aatttgatct atttccacaa
    50941 tacgacgaag aaaacaaaac agtaaatgca aagctgatct accatccgga tacattagaa
    51001 ataattggcg gacagctgat aagccaagaa ttcttattat cagatttgaa cctgcttgcg
    51061 gatatcgtga aacacaaaac tacgattcca cagctagcag ttgaggattt tggatttctg
    51121 tctgaataca cctctcgttt tcactacctc aatgagttag cttttaaagt actagctaag
    51181 agagcaaata gcaatagagt agataaaaga ctgtgagaaa tatcacggtc ttttattgtg
    51241 tggggatata ataaatatta atcaatcgag aataaaaaat tcatataatg aaatagcgga
    51301 agaatttttt gagcataata ttcgccaaaa atttatcaag tgataaaatt gttgtataaa
    51361 atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa
    51421 agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt
    51481 tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg
    51541 cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta
    51601 agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccaa acacctggag
    51661 tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg
    51721 ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg
    51781 aggcagtagg cgatattagt ttgaaagaag aatatggtct agccatagga gatacagtta
    51841 agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca
    51901 atgtctcacc agttctctat acaaccatca atgcttacca agaaatccgc ttcgaaaaag
    51961 aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt
    52021 tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata
    52081 atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg
    52141 tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga
    52201 aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt
    52261 tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac
    52321 ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgt
    52381 tagtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt
    52441 tgaaggcaat tggataagaa ggaggaagaa catgaaagca atagaattta aagaagtaga
    52501 aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat
    52561 cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt
    52621 gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga
    52681 attcagtaat caaaaagaaa gcgaacgcgt taaactacgt tttaaagaaa ttggctttat
    52741 tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga
    52801 taagattaaa catgaacatc gtcaaaaaga agcaaacgaa ttatttgagc agcttggcgt
    52861 ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc
    52921 aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag
    52981 tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa
    53041 aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat
    53101 atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa
    53161 tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt
    53221 ttatgtccaa gggatatctc ggcacttcaa caagagaaat cgcacaaaaa gttgggatca
    53281 cccagcccaa tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag
    53341 aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg
    53401 gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt
    53461 tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt
    53521 tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat
    53581 ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag
    53641 caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga
    53701 tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaat
    53761 agttaggtga agaagcaaga tatgacgaag atcgttatat cttgcttctt tatataggta
    53821 gtttttcata aagacaaatt tgttattctc tcatgattat cagggtatat ttttatttat
    53881 tatgtcggtg ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa
    53941 tgaaggaaac tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg
    54001 atatgccaaa aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt
    54061 ttacagcccc ttttttgctt acttaagctg attttaagtt ttggccctta gtctggatct
    54121 acgaggtgag agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc
    54181 attattatct gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc
    54241 agcagtacga acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag
    54301 acattaatca aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc
    54361 ccacttgcaa ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct
    54421 tatcagatca agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa
    54481 gtcacaaaga atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat
    54541 cctgggcatg gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa
    54601 gatataacat taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg
    54661 gtttatttga cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt
    54721 gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat
    54781 gaagcaactg gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca
    54841 attgctgact ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat
    54901 caagttcttc gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat
    54961 gatagtgatt taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg
    55021 acacaagcat tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc
    55081 ctcatatttt tcgtttggcc gggcttatta tcgcagatgc aagcttttga acaccgttta
    55141 tttttttagt agcttcaaag ctttttctgt atttgcaaag tgaagtttgg catatttttc
    55201 aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc
    55261 agctcctgat ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa
    55321 gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt
    55381 aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt
    55441 tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt
    55501 tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt
    55561 tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt
    55621 ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac
    55681 accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag
    55741 cggaccgaaa tagctgccat ttccgacctc atcactacct aaaacggata attgactgat
    55801 attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct gtgcctcccc
    55861 ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc cggatgtata
    55921 agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa tctgtttatt
    55981 taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg ttgctttgat
    56041 aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc gcaactcacg
    56101 acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta tcatataata
    56161 gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca tggtacacta
    56221 aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac
    56281 aaagcggtga ttgccaacca aacatatact attatcggac gggaaaccaa gcatcatatg
    56341 gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt atcaccacaa
    56401 atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga tcaattgaag
    56461 aaacaagaaa gaatcttgga gctggaagaa gaaacagcag aattaaagaa aaaaatgatc
    56521 aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg
    56581 gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt ggaagcacaa
    56641 cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa ttaagatcct
    56701 tttatcaaaa taagaaaaat ttaaaaaaag ttagaagaga aaggaacgta gcatgctgag
    56761 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
    56821 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
    56881 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca cgccaacatc
    56941 aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg
    57001 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
    57061 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
    57121 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
    57181 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgct ttatcgttga
    57241 gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta
    57301 aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagattatt taagaaggct
    57361 gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa
    57421 taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt
    57481 agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagcg aggaacaaac
    57541 gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg
    57601 gatccctata cctaaacttg aaaatatccg tccccatatg aaacggattg agatcggagc
    57661 agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt
    57721 gaatcgtttc attgatgatc tttatgacag tgaaatcgaa tttgcacgtt tgtatatgtg
    57781 ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga
    57841 agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg
    57901 gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa
    57961 gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca
    58021 agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt
    58081 atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc
    58141 tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg
    58201 tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc
    58261 acgatttggt aaagaagtaa aagctatcgt gccgggaatc agtgaagaca atcatgtcgt
    58321 cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac
    58381 aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat
    58441 cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc
    58501 aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca
    58561 gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt
    58621 atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc
    58681 gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta
    58741 tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac
    58801 gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat
    58861 tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga
    58921 agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat
    58981 cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt
    59041 tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga
    59101 agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc
    59161 ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca
    59221 aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca
    59281 agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa
    59341 accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa
    59401 cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct
    59461 gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga
    59521 atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct
    59581 aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat
    59641 tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca
    59701 tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat
    59761 tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa
    59821 atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca
    59881 gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact
    59941 tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat
    60001 gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa
    60061 ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa
    60121 gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa
    60181 cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc
    60241 ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga
    60301 ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat
    60361 gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat
    60421 gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt
    60481 ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga
    60541 agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct
    60601 gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt
    60661 ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga
    60721 caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat
    60781 cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct
    60841 ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta
    60901 tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc
    60961 agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga
    61021 ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac
    61081 tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca
    61141 agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta
    61201 taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca
    61261 tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt
    61321 gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc
    61381 gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga
    61441 acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg
    61501 tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt
    61561 attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa
    61621 cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat
    61681 tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga
    61741 tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc
    61801 aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc
    61861 gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg
    61921 attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga
    61981 tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt
    62041 gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt
    62101 cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa
    62161 gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata
    62221 ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga
    62281 aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt
    62341 ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa
    62401 gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg
    62461 gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg
    62521 gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg
    62581 gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa
    62641 agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct
    62701 gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt
    62761 agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc
    62821 atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat
    62881 catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt
    62941 tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga
    63001 aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc
    63061 tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa
    63121 ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta
    63181 agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga
    63241 tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc
    63301 aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc
    63361 aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag
    63421 cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg
    63481 ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga
    63541 tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac
    63601 cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg
    63661 attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt
    63721 atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag
    63781 agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac
    63841 aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat
    63901 tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa
    63961 atatattaga cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc
    64021 tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg
    64081 tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg
    64141 gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca
    64201 cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag
    64261 tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga
    64321 tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat
    64381 ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca
    64441 atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc
    64501 tattttattt attttactat attggatata tattcattga aaatagtgta taacaacttt
    64561 ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt
    64621 tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt
    64681 ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct
    64741 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt
    64801 tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa
    64861 tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg
    64921 atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg
    64981 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga
    65041 aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa
    65101 tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt
    65161 ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt
    65221 tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc
    65281 tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa
    65341 ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat
    65401 cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa
    65461 gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta
    65521 ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat
    65581 gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa
    65641 acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt
    65701 ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa
    65761 aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta
    65821 gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
    65881 ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat
    65941 ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag
    66001 agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa
    66061 agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa
    66121 ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa
    66181 aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc
    66241 aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt
    66301 ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc
    66361 gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat
    66421 acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat
    66481 cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga gactgagaca
    66541 aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc
    66601 acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc
    66661 agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc
    66721 ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat
    66781 actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca
    66841 aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat
    66901 tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaat gagtccatcg
    66961 ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa
    67021 gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg
    67081 acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt
    67141 ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt
    67201 ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt
    67261 tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa
    67321 agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt
    67381 tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa
    67441 actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt
    67501 gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa
    67561 gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac
    67621 ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga
    67681 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    67741 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    67801 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    67861 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    67921 cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa
    67981 cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt
    68041 tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga
    68101 aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc
    68161 aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    68221 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    68281 atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag
    68341 aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat
    68401 cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    68461 aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc
    68521 gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa
    68581 ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    68641 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    68701 agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg
    68761 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    68821 gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    68881 gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag
    68941 cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa
    69001 tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc
    69061 catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    69121 ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca
    69181 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa
    69241 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca
    69301 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat
    69361 ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg
    69421 atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg
    69481 atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct
    69541 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    69601 ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa
    69661 gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt
    69721 attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg
    69781 ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa
    69841 gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat
    69901 ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca
    69961 ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg
    70021 atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc
    70081 gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt
    70141 gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa
    70201 taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta
    70261 tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt
    70321 gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg
    70381 caggctctga ttctgcaatt gcttccaggt acgcaaggtt tgtatggatt cgttattgcc
    70441 tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta
    70501 ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc
    70561 gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    70621 tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg
    70681 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    70741 agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga
    70801 tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag
    70861 aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag
    70921 aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg
    70981 cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat
    71041 tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct
    71101 atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa
    71161 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    71221 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    71281 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    71341 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    71401 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    71461 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    71521 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt
    71581 ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta
    71641 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    71701 aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    71761 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    71821 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    71881 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    71941 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    72001 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    72061 ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta
    72121 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    72181 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    72241 aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    72301 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    72361 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    72421 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    72481 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    72541 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    72601 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    72661 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    72721 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    72781 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    72841 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    72901 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    72961 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    73021 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    73081 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    73141 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    73201 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    73261 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    73321 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    73381 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    73441 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    73501 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    73561 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    73621 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    73681 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    73741 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    73801 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    73861 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    73921 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    73981 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    74041 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    74101 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    74161 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    74221 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    74281 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    74341 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    74401 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    74461 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    74521 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    74581 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    74641 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    74701 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    74761 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    74821 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    74881 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    74941 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    75001 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    75061 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    75121 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    75181 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    75241 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    75301 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    75361 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    75421 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    75481 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    75541 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    75601 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    75661 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    75721 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    75781 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    75841 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    75901 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    75961 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    76021 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    76081 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    76141 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    76201 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    76261 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    76321 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    76381 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    76441 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    76501 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    76561 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    76621 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    76681 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    76741 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    76801 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga
    76861 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    76921 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    76981 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    77041 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    77101 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    77161 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    77221 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    77281 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    77341 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    77401 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    77461 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    77521 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    77581 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    77641 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    77701 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    77761 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    77821 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    77881 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    77941 ttttattgaa a
//
LOCUS       DADVXN010000002        75385 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.002, whole genome shotgun sequence.
ACCESSION   DADVXN010000002 DADVXN010000000
VERSION     DADVXN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 75385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.002"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(154..279)
                     /locus_tag="KB942_000080"
     CDS             complement(154..279)
                     /locus_tag="KB942_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6850538.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(413..1306)
                     /locus_tag="KB942_000081"
     CDS             complement(413..1306)
                     /locus_tag="KB942_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBB6850539.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1488..2414)
                     /locus_tag="KB942_000082"
     CDS             complement(1488..2414)
                     /locus_tag="KB942_000082"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBB6850540.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2499..3260)
                     /gene="pflA"
                     /locus_tag="KB942_000083"
     CDS             complement(2499..3260)
                     /gene="pflA"
                     /locus_tag="KB942_000083"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBB6850541.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3504..5735)
                     /gene="pflB"
                     /locus_tag="KB942_000084"
     CDS             complement(3504..5735)
                     /gene="pflB"
                     /locus_tag="KB942_000084"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBB6850542.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6012..8462)
                     /gene="parC"
                     /locus_tag="KB942_000085"
     CDS             complement(6012..8462)
                     /gene="parC"
                     /locus_tag="KB942_000085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBB6850543.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8474..10519)
                     /gene="parE"
                     /locus_tag="KB942_000086"
     CDS             complement(8474..10519)
                     /gene="parE"
                     /locus_tag="KB942_000086"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBB6850544.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10685..11119)
                     /locus_tag="KB942_000087"
     CDS             complement(10685..11119)
                     /locus_tag="KB942_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBB6850545.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11246..11884
                     /gene="plsY"
                     /locus_tag="KB942_000088"
     CDS             11246..11884
                     /gene="plsY"
                     /locus_tag="KB942_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBB6850546.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12016..12891)
                     /locus_tag="KB942_000089"
     CDS             complement(12016..12891)
                     /locus_tag="KB942_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBB6850547.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(12973..13764)
                     /gene="codY"
                     /locus_tag="KB942_000090"
     CDS             complement(12973..13764)
                     /gene="codY"
                     /locus_tag="KB942_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBB6850548.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13782..15182)
                     /gene="hslU"
                     /locus_tag="KB942_000091"
     CDS             complement(13782..15182)
                     /gene="hslU"
                     /locus_tag="KB942_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBB6850549.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15196..15744)
                     /gene="hslV"
                     /locus_tag="KB942_000092"
     CDS             complement(15196..15744)
                     /gene="hslV"
                     /locus_tag="KB942_000092"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBB6850550.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15781..16686)
                     /gene="xerC"
                     /locus_tag="KB942_000093"
     CDS             complement(15781..16686)
                     /gene="xerC"
                     /locus_tag="KB942_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBB6850551.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16884..18260)
                     /gene="trmFO"
                     /locus_tag="KB942_000094"
     CDS             complement(16884..18260)
                     /gene="trmFO"
                     /locus_tag="KB942_000094"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBB6850552.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18229..20307)
                     /gene="topA"
                     /locus_tag="KB942_000095"
     CDS             complement(18229..20307)
                     /gene="topA"
                     /locus_tag="KB942_000095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBB6850553.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20429..21286)
                     /gene="dprA"
                     /locus_tag="KB942_000096"
     CDS             complement(20429..21286)
                     /gene="dprA"
                     /locus_tag="KB942_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBB6850554.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21342..22109)
                     /locus_tag="KB942_000097"
     CDS             complement(21342..22109)
                     /locus_tag="KB942_000097"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBB6850555.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22102..22962)
                     /gene="ylqF"
                     /locus_tag="KB942_000098"
     CDS             complement(22102..22962)
                     /gene="ylqF"
                     /locus_tag="KB942_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBB6850556.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23222..23620
                     /locus_tag="KB942_000099"
     CDS             23222..23620
                     /locus_tag="KB942_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6850557.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            23899..24399
                     /locus_tag="KB942_000100"
     CDS             23899..24399
                     /locus_tag="KB942_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6850558.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(24482..25033)
                     /gene="lepB"
                     /locus_tag="KB942_000101"
     CDS             complement(24482..25033)
                     /gene="lepB"
                     /locus_tag="KB942_000101"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6850559.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25185..25412)
                     /locus_tag="KB942_000102"
     CDS             complement(25185..25412)
                     /locus_tag="KB942_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBB6850560.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25409..25927)
                     /gene="msrA"
                     /locus_tag="KB942_000103"
     CDS             complement(25409..25927)
                     /gene="msrA"
                     /locus_tag="KB942_000103"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6850561.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(25947..26660)
                     /locus_tag="KB942_000104"
     CDS             complement(25947..26660)
                     /locus_tag="KB942_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBB6850562.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26663..27559)
                     /locus_tag="KB942_000105"
     CDS             complement(26663..27559)
                     /locus_tag="KB942_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6850563.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27717..28559)
                     /locus_tag="KB942_000106"
     CDS             complement(27717..28559)
                     /locus_tag="KB942_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6850564.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28691..29344
                     /locus_tag="KB942_000107"
     CDS             28691..29344
                     /locus_tag="KB942_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6850565.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29407..29934)
                     /locus_tag="KB942_000108"
     CDS             complement(29407..29934)
                     /locus_tag="KB942_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6850566.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29954..30901)
                     /locus_tag="KB942_000109"
     CDS             complement(29954..30901)
                     /locus_tag="KB942_000109"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6850567.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(30913..32799)
                     /locus_tag="KB942_000110"
     CDS             complement(30913..32799)
                     /locus_tag="KB942_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6850568.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(32959..35037)
                     /locus_tag="KB942_000111"
     CDS             complement(32959..35037)
                     /locus_tag="KB942_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6850569.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35050..35325)
                     /locus_tag="KB942_000112"
     CDS             complement(35050..35325)
                     /locus_tag="KB942_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850570.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(35687..36142)
                     /locus_tag="KB942_000113"
     CDS             complement(35687..36142)
                     /locus_tag="KB942_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850571.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36296..37504)
                     /locus_tag="KB942_000114"
     CDS             complement(36296..37504)
                     /locus_tag="KB942_000114"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6850572.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(37616..37948)
                     /locus_tag="KB942_000115"
     CDS             complement(37616..37948)
                     /locus_tag="KB942_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6850573.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38096..38971
                     /locus_tag="KB942_000116"
     CDS             38096..38971
                     /locus_tag="KB942_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6850574.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39053..39634)
                     /locus_tag="KB942_000117"
     CDS             complement(39053..39634)
                     /locus_tag="KB942_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6850575.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(39640..40899)
                     /locus_tag="KB942_000118"
     CDS             complement(39640..40899)
                     /locus_tag="KB942_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6850576.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(40901..41947)
                     /locus_tag="KB942_000119"
     CDS             complement(40901..41947)
                     /locus_tag="KB942_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850577.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42182..42457)
                     /locus_tag="KB942_000120"
     CDS             complement(42182..42457)
                     /locus_tag="KB942_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6850578.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(42700..44010)
                     /gene="der"
                     /locus_tag="KB942_000121"
     CDS             complement(42700..44010)
                     /gene="der"
                     /locus_tag="KB942_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB6850579.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44192..45421)
                     /gene="rpsA"
                     /locus_tag="KB942_000122"
     CDS             complement(44192..45421)
                     /gene="rpsA"
                     /locus_tag="KB942_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB6850580.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(45556..46236)
                     /locus_tag="KB942_000123"
     CDS             complement(45556..46236)
                     /locus_tag="KB942_000123"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB6850581.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46285..46920)
                     /locus_tag="KB942_000124"
     CDS             complement(46285..46920)
                     /locus_tag="KB942_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6850582.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(46986..48389)
                     /locus_tag="KB942_000125"
     CDS             complement(46986..48389)
                     /locus_tag="KB942_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBB6850583.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48376..49407)
                     /locus_tag="KB942_000126"
     CDS             complement(48376..49407)
                     /locus_tag="KB942_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850584.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            49438..49659
                     /locus_tag="KB942_000127"
     CDS             49438..49659
                     /locus_tag="KB942_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBB6850585.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(49824..50561)
                     /locus_tag="KB942_000128"
     CDS             complement(49824..50561)
                     /locus_tag="KB942_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBB6850586.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(50714..52054)
                     /locus_tag="KB942_000129"
     CDS             complement(50714..52054)
                     /locus_tag="KB942_000129"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBB6850587.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52291..53814)
                     /locus_tag="KB942_000130"
     CDS             complement(52291..53814)
                     /locus_tag="KB942_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBB6850588.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54192..54803)
                     /locus_tag="KB942_000131"
     CDS             complement(54192..54803)
                     /locus_tag="KB942_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6850589.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(54926..55051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55133..55849)
                     /locus_tag="KB942_000132"
     CDS             complement(55133..55849)
                     /locus_tag="KB942_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6850590.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(55855..56424)
                     /gene="scpB"
                     /locus_tag="KB942_000133"
     CDS             complement(55855..56424)
                     /gene="scpB"
                     /locus_tag="KB942_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBB6850591.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(56408..57205)
                     /locus_tag="KB942_000134"
     CDS             complement(56408..57205)
                     /locus_tag="KB942_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBB6850592.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57175..57546)
                     /locus_tag="KB942_000135"
     CDS             complement(57175..57546)
                     /locus_tag="KB942_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6850593.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(57565..58452)
                     /gene="xerD"
                     /locus_tag="KB942_000136"
     CDS             complement(57565..58452)
                     /gene="xerD"
                     /locus_tag="KB942_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBB6850594.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(58548..59021)
                     /locus_tag="KB942_000137"
     CDS             complement(58548..59021)
                     /locus_tag="KB942_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6850595.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59126..59989)
                     /locus_tag="KB942_000138"
     CDS             complement(59126..59989)
                     /locus_tag="KB942_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6850596.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60185..62284)
                     /locus_tag="KB942_000139"
     CDS             complement(60185..62284)
                     /locus_tag="KB942_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6850597.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(62504..63613)
                     /gene="rpoD"
                     /locus_tag="KB942_000140"
     CDS             complement(62504..63613)
                     /gene="rpoD"
                     /locus_tag="KB942_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBB6850598.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(63637..65478)
                     /locus_tag="KB942_000141"
     CDS             complement(63637..65478)
                     /locus_tag="KB942_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBB6850599.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(65660..67975)
                     /locus_tag="KB942_000142"
     CDS             complement(65660..67975)
                     /locus_tag="KB942_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6850600.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68160..69020)
                     /locus_tag="KB942_000143"
     CDS             complement(68160..69020)
                     /locus_tag="KB942_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBB6850601.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69079..69600)
                     /locus_tag="KB942_000144"
     CDS             complement(69079..69600)
                     /locus_tag="KB942_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBB6850602.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            69878..70639
                     /locus_tag="KB942_000145"
     CDS             69878..70639
                     /locus_tag="KB942_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6850603.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            70665..73256
                     /gene="mprF"
                     /locus_tag="KB942_000146"
     CDS             70665..73256
                     /gene="mprF"
                     /locus_tag="KB942_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6850604.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(73444..74631)
                     /gene="tuf"
                     /locus_tag="KB942_000147"
     CDS             complement(73444..74631)
                     /gene="tuf"
                     /locus_tag="KB942_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6850605.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            74999..75355
                     /locus_tag="KB942_000148"
     CDS             74999..75355
                     /locus_tag="KB942_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6850606.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20604 a        15901 c        12957 g        25923 t
ORIGIN      
        1 aaaagcattt tgaagcctga ctattacgac ttcataaatg cttttttagt tttaaacact
       61 tcaatctaaa agaataaccc aagattggat aaattattaa tgactcgcca ttcgtttctt
      121 agtgattctg gattatcaaa tggacttcct aatctaatca gctagttctt cccataaaat
      181 gccatacttg actgaatcat aatattgatt atttaaaaat ctaacttttc ttatcactac
      241 ttcctttgtc attccacatt tctcaccatt tttttgcatt cctttgtttc cagaccatgt
      301 agtgaatcta ataggtggta gataggcaaa atactcaaat aattctttta accataaagg
      361 aagagaaagg ctcctcctgc aagagtcagt ataatttttg cttactgctc tcttaggttt
      421 tactcactaa aactgttcca tattctttga tataagaaaa tgcatgcggt ataaatttct
      481 tgctcagctc agaaaagatt tgtcgtttaa gaaaggccat ttcctccatt gtcaatgaag
      541 gatccaatgt tggtaattca atctcaaaat tgtttttttc gacaataggt aattctaatg
      601 tgaatgattc ttcttccttt atagtattcg tcacaagtag agactgatgg aaaatactag
      661 gccggcgatt tttatattta ttcttacgta tcctgtcaac gataacttct atttgatcaa
      721 aaaagtacga ttcatttacg tctccaataa gttcggctaa ttctttgaat gccttcgtta
      781 caggaagctg attcttctgt acaaaaaagt aattagccaa agtaagcggg aagtgttcta
      841 tagaggaaag tgtgataaaa cggtacttct catttcctgc agttgtcctc agcaattctg
      901 tctctcggct gtttcttact ccataaaaat acgcttcttt ccgttcatct tcgaaacaat
      961 acgcccaaat aacttctccc atcgctaatt tttgttcatc aacagagagt ctcttcaatt
     1021 gttcagctat ggtttcagca gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata
     1081 ttggaaagtt caaatgatat cttcgttctt ccctcttgat taatcctgca tcgatcaata
     1141 aatcaagaaa atgggaaaat ttattttgcg aaaattcttt tttcaattct cttagaatcg
     1201 tttcttgttc tcgatgttca tatagataat cggtcaatga agaaaaaagc ggatcatcgg
     1261 tcagtttttg ataaaagttt tccttatttt ttgaataata gttcatatat gttctctttt
     1321 ctatccgttt tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat
     1381 aattgactgt tattctcctc catttcttcg gcctttagta tgttctatgt ttctttttat
     1441 atcaatcgct tatccattca ttttgaatgc ttcatactac catttgatta gctaaataca
     1501 gccgtcaatt gaggtacgac ttgtttttta cgtgaaacga cacctttaag caaagcacgg
     1561 ttgttggtca aagttgtttg gaaagcttct tctacttttt cttttccttt accagcaaca
     1621 agtaattctg aatcactgtc taagatgttt gtgacaacta atacgaacag atcgtagcca
     1681 tttgctttca tttcatcttc cattgctgct tctagctctg cttgtcgatc catcacttca
     1741 ttaaggtcca ctgtattgac ttgagcgata cgaacgttgg aactgcccat tgggaaactt
     1801 tttgcatcca aatcaagaag aaccgcagct gatttatcac ttagatttgt tcctgctttc
     1861 aacatttcaa gtccataagt gtttagatcg acttcagcga ttgctgcaag ttcttttgca
     1921 gctgcttcat cttcaggcgt acatgttggt gatttgaaca acaatgtatc tgaaatgata
     1981 gctgagagca tgattcctgc gatttcttta ggaattgtta cttggttttc tttatatagt
     2041 tttaaaataa tcgtacttgt acatccaact ggttctgcac gatagtacaa tggattagct
     2101 gtttcaaagt ttgcaatacg atggtggtct actacagcta aaatatttac ttcgctgata
     2161 tctgaaatac tttgttggaa ttcattatga tccacaagca tgacatctgc attttctgct
     2221 ttagctgact ctactactcg tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt
     2281 tcttcattag gtgtacctag agcaactgct tctgtttctt tacctaattc attttgcaag
     2341 tgtgaaaacg caatggctgc cccgattgca tctgtatctg gattttggtg accaaaaact
     2401 aaaatttttg acatgtcatt cgctcctttg tgatttctcc tatttaataa tagcaaaaaa
     2461 acggaaagaa gcaatactta tcttctttcc gatgcgacct atcgcgttaa atattttgta
     2521 tactcttctg tatgaagcaa tcgtttagca ttttcgacac gttcttttgt aggtggttcg
     2581 atcccttcta atggatattt caaaccaagt gtttcccact tgtattttcc catcgtatga
     2641 taaggaagaa tctctactct gtctacattt gacaaggttt gaataaagtt gtctagtcgg
     2701 atcaaatatt catcataatc acttcgctca ggaactagta catggcgaat ccaaaccggt
     2761 ttgttgattt ttgacaaata ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt
     2821 agttttttat gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac
     2881 ttcatcaatt cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat
     2941 gtatccaatg tagtatgcat cttccgctct tttgcttttt taaatagttc gatcaaaaaa
     3001 tcgatctgca atagaggttc tccaccacta acagtaatac cacctttatc tccccagtaa
     3061 ggtctgaagc gttctgcttt atctaaaagt tcatctgctg tgtattcttt ccctccgcca
     3121 atattccaag tatctggatt gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac
     3181 acgacaaagc gcagtccagg tccgtctact gaaccgaaag tttcgataga atgaacatag
     3241 ccgattgttt tttcttccat ttaagttcac atcttttctt tgatttcatt ttaagtacaa
     3301 gttagctgtc gaagcgaagc tttctgaaca aaatttttcg caattgccgc ttcgttatgt
     3361 gtaaaattta cagggacgga acatacaagt cctgcccctg taaatcgttt ctttcaaata
     3421 agcatactga ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc
     3481 gaaaaacttt tttcacgaac tttttacatt ctgtcgtgtg aagttctcat caagacatct
     3541 aattgttgtt ctcttgtcaa gtcacggaat ttaactgcat aaccagatac ccggatcgtc
     3601 aagttcggat atttcattgg attttccatt gcatcacgca ataaatcatt actgaataca
     3661 ttgacattca aatgataagc tccttttgag aagtatccat ctaagacatt acgcaagttt
     3721 tcgattcttg tatcttgttc ttttccaaga cctgaaggat tgattgtttg ggtatttgaa
     3781 attccatcca atgcacagcc gtaattcatt ttagctgttg agttcaggct agctaataac
     3841 ccactttgtt ccgcattgta acttggattt gctcctggcg caagtggttt tccagcttca
     3901 cggccatcag gagtatttcc tgtattttta ccgtaaacaa catttgaagt aatcgtcaat
     3961 aatgaagttg ttggttttga gttgcggtaa gttttatgac gtttgatttg tcccatgaag
     4021 tattccaaga tccatgaaac taattcatct gcacggtcat catcatttcc gtatttaggg
     4081 aagtcgccat ccacttcaaa atcaactgct attccttttt cattacggat tgcttttact
     4141 tttgcatatt tgatcgctga caagctgtct gccgcatgag agatcccagc aatacctgta
     4201 gcaaatgtgc gttgcaagtc agaatccatc aaagccagct gagctgcttc ataagaatat
     4261 ttatcatgca tataatgaat gatgttcaac gtattgacat atagttctgc taaccaatcc
     4321 atcatattca tgaagttatg tttcacttct tcaaagtcta gtatttctga tgtgattgga
     4381 cgatattctg gtccaacttg ggcgccagtc acttcatcga ctccgccatt gatggcatat
     4441 aatagtgttt tagccaagtt agcacgagcg ccaaagaatt gcatgtcttt tccgacaact
     4501 gttcctgata cacagcaagc aatcgcacaa tcatcagaac cccattgttc acgaagctga
     4561 ttatcatttt cgaattgaat cgaagaactt tcgatagcga ttctgctagc atatgttctg
     4621 aatccttctg gaaggtgttc agaatacaaa acagtcaagt ttggttctgg tgaaggtccc
     4681 atatttgtta atgtatgaag cagacggaaa tcattctttg taactaatga acgaccatct
     4741 aatcccatac ctgcaatgga taaagtagcc cagataggga atcctgagaa taattggtta
     4801 tattcaggtg tacgcgcgaa tttgaccata cgtaatttca ttactaagtg gtcgatcaat
     4861 tcttgtgctt ctttttctgt gattactcca ttatccaaat cacgttggat atagatatca
     4921 aggaatgctg agatacgtcc gattgacatc gcagcaccat tttgtgattt gattgctgct
     4981 aaatatccga agtatagcca ttggacagct tcttttgcat ttgcagctgg tttagaaata
     5041 tcatagccat atttcgcagc catttctttc atttgcgcta atgcacgata ttgttcagtg
     5101 atttcttcac gtaaacgaat cacttcttca ctcattgtat gataaccagt catatcatga
     5161 tcttttttct tttgttccat caaatagtcg attccataca atgccacacg acgatagtca
     5221 ccgatgatac gtccgcgacc gtaagcgtct ggaagccctg taatgatttt gtttttacga
     5281 gcagcacgca tttcagcagt ataagcgtca aatacacctt gattatgtgt tttgcgccag
     5341 tcagtgaaaa tatgtgtcaa ttcatcgttt ggtgtatagc cattgctttt tagcgcattg
     5401 ttagccatat tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt
     5461 agaccgacga ttttttccaa atctttatcc aaatagcctg catcgtgtga agtaatagtt
     5521 gatacgattt ctgtgtccat atcatagaca ccgttacgct cgaattggat atcatttaac
     5581 ttttgcaatt catcccataa tttttctgtt gcttctgttg ggccttccaa aaagctgtca
     5641 tcaccgcgat attcagtgta gttattttga ataaagtctc tagtgtctac gccttctttc
     5701 catttggtac ctttaaatcc attccattgc tccattgtat ggacctccta aatttctgtt
     5761 ttatgtaaca gtttttacgt gattagtata acacagcacc ctatctttgc aagggttttc
     5821 ttgtttttta tttaacaatt tttatgtttt atttacatat atttaaatca acgttgacat
     5881 accaacattt aagcattgtg aacactttca tctttttttg caaacgtata aggatttatt
     5941 aaaaatgaat gaaaaagaga cgccacctgt attaatacag atggcgtctc ttcattaaaa
     6001 tactacaaca gttacgactc gatgaaaatc tcaggagcat cgataacttc gatgacttct
     6061 ccaccttttt tctcatccat cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa
     6121 tcagttgggt caatcgttaa ctgctgccct ttttgattca tcaaggttat ttcttttgat
     6181 cttccttccg acatatagat aatacggtgc ggattttttt tcagctcacg catgaccgtc
     6241 actccccgct tcgcacgtga agtttggctg atttcttgag caagcatgcg tttaacagct
     6301 cctcgctgag taacgattac gatcggcgta tcaccttctg ctaacacaag cagcccgttg
     6361 ataatcctat ctccatcttt caaattcata gacttcactc cagctgcctt tgctccaaca
     6421 acgggaacat ctgttaaagg atagcgcaaa cccattcctt gatgcgttac taagaaaaca
     6481 tctaagcctg gctgatcttc tttcagatac acagcaatca gttcatcttc ttccgccttt
     6541 agcttcatac agctgagcgg acggctctta taggtgcgcc atggttcgaa ctctgtcatt
     6601 cgtgtttgct tgatcaatcc attcttgctg atgaatacaa atgttttctc tgcttgaatt
     6661 ttttgatatg ggaaaacagc taaaatcgat tcgtctacag acagattgga gattgtttga
     6721 gaaatgtgct caccagcatc tttccatttc aaatcgggta gttcatggac agggcggtag
     6781 atcacatttg ctttatttgt gatcagaaga acatggtcca atgtactcaa ttcacctgag
     6841 tatagcaagt agtcgccttc tcgcatcccg atttcttctg gtttggaagc actataagaa
     6901 cgaaggcttg ttcgcttcac atagccttct cgcgtcacac tgacaattac gtcttcttgt
     6961 gccactaaga cttgcgtatc aattttgatt tcttcgattt cgtcttcgat tgtcgttaga
     7021 cgagcggaag aataattttt tttcacttct cgcaattctt ttttcataac agagaaaagc
     7081 tctttgtcat ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa gctttcggat
     7141 tccttttgaa gctctgtgat atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt
     7201 gcttctgctt gttcttctgt aaattgaaag acgtcgacta aattcttttt ggcgtctttt
     7261 ttgtctttac tttcacgaat cgttgcgatt acttcatcca gaatcgataa agctttcatc
     7321 aatccttcta cgatatgctg tcttttttga gctttggcta aatcgaattg actacgtttt
     7381 gttatgactt gctttcgatg ctcgatataa cttcttaaaa tgtctttcag tccaacttga
     7441 tgcggagtca tatggtcgat agcgaccata ttgaaattgt aattgatctg tagctcagtg
     7501 tttttgaaca aataattcaa aattccttga gcattggcgt cttttttcaa ttcgacgaca
     7561 atttgcagac ctgttctatc actttcatct cgtacttcag ctattccatc gattttctta
     7621 ttcaaacgga tctcatccat ttttttcacc aaggtcgctt tatttacttc atatgggatt
     7681 tctgtgatca cgatttgctg tttattaccc ttcattgcct ctatctttgt tttcgaacga
     7741 agaataactt ttcctcgtcc tgtttcataa gcttttttga tttcatcttt tccttgcaag
     7801 atccctcctg ttggaaagtc aggtccagga atatattcca tcaatttttc aaggctcgct
     7861 tgtggatgat cgatcatata gattgtgcca tcgatcactt cagctaagtt atgagtcggg
     7921 atttctgttg catatccagc agatatacct gtcgatccat tgacgagaag gtttgggtat
     7981 cgagctggaa ggactgttgg ttctttttct gtatcatcaa aattccacac gagatcgact
     8041 gtttctttct cgatatctgc tagcatctct ccacttaact tggataatct ggcttcagta
     8101 taacgcatcg cagcaggtgg atcaccgtcc atactcccgt tattcccgtg catttcgatc
     8161 agtacttccc gcaacttcca gtcttggctc atgcggacca tcgcttcata aatactgatg
     8221 tcaccatgag gatgataatt ccccatgata tttccgacag attttgctga tttacggaat
     8281 cctttgtcat acgtattgcc atctttgttc atggcaaata atatccggcg ttgcactggc
     8341 ttcaatccat cacggatatc aggcagtgca cgttcttgga taatatattt ggaataacgt
     8401 ccgaagcggt cacccatcac ttcttctaaa gttaattctt gtacttcttg tcgattttcc
     8461 atcaggtgac ctcctattct acttcaaata actgattgtt ttcattttta tctgctcgtt
     8521 cttcatctaa taaatcatta gaaactgaag gagagatttc agtatcctct tttttatcta
     8581 agatactgcc atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag
     8641 gttctacttt gtcacccatc agtgtggtta ctcgacgttc agcttgcgca gcatcatcga
     8701 tacggacgcg gatcaacgta cgggaagtcg gatccatcgt tgtttcccac aattgttccg
     8761 cattcatctc tccaagtcct ttatatcgct gcagcatata tcctttgccg acttttttaa
     8821 tcattgctgc caattcgtca tctgtccacg catattcaat gacttgcttc tttccttgtc
     8881 ctttggatac tttatataaa ggcggtaacg cgatatatac tttcccagct tcaatcagcg
     8941 gtttcatata acgataaaag aaagtaagca gtaatacttg aatatgggcg ccgtctgtat
     9001 ccgcatcggt catgatgatt actttgtcgt aattacagtc ttcgatagaa aactcaggac
     9061 cgactcctgc accaatcgta tagatcatcg tattgatttc ttcatttttc aagatatctt
     9121 gcattttagc tttttcggta ttgatgactt ttcctcgtaa aggtagaatt gcttggaact
     9181 ttctgtctct tccttgttta gcagatccgc cagcagaatc cccttcgact aaatacaatt
     9241 catttttctt agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc
     9301 cttttttacg cttcttccca tttcggcttt cttctctggc tttacgtgct gcttctctcg
     9361 cttctctggc tttgattgct ttacggacca acatctggct catttcgcta ttttcctgca
     9421 aataaaagcc catttgttca ctgatgacgt tatctactac ggtacgagca actggcgtac
     9481 caagtttttc ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa
     9541 gtacagcagc taacccttct ctgaaatcac taccttccag attcttgtcc cgttctttca
     9601 gcaaaccaac ttttctcgca tattcattat aagatttcgt catcgccgct ttcataccag
     9661 cttcatgggt tcccccatct tttgtccgaa cgttattgac aaaagataaa acattttctg
     9721 aataaccgtc attgtattga taagcgacct ctacttcgat accttctttt tctcctgaaa
     9781 aataaacaac tggtgtcaat gtatcttttt cttcattcag ataatcaaca aattctttga
     9841 ttccttcttc gtaatgaaaa atttccttga ctggttcttc tccacgaaga tcagaaagag
     9901 agattttaac gccttttaga agaaaggcag attctcttaa ccgttcggct aaaatttcat
     9961 atgaaaattt cgttgtactg aatattgtat catctggtaa aaaatggaca gtcgtcccgt
    10021 ttgctttttt tgtttttcct atctttttca aagtaccgtc aggtttaccg ccgtttttaa
    10081 atttctgctg atactctacg ccatctctaa cgatcgttac aattagccat ttcgataagg
    10141 catttaccac actggctcct acaccgtgca gtccaccaga agttttgtac cctccttgac
    10201 caaactttcc gccagcatgc agcacagtaa aaatcacttc aactgttggt attccggaag
    10261 catgcattcc tacaggcatc ccacgtccgc tatctgcaac agttatacta ttgtcttcat
    10321 ggatggtcac atcaatctca ctgccataac ctgataatgc ttcgtcaact gcattatcga
    10381 cgatttcata gactaaatga tgcaagcctc ggctgtcagt ggatccaatg tacatccctg
    10441 gtctttttct aactgcctct aatccttcca atacttggat ggacgagtca ttgtattcgt
    10501 tgtttacttt ttttgccaag gcaaacgctc cttcaataat actttgcggc aaaaccgcac
    10561 cacgattatc atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt
    10621 aattatctcc taaccgcttt ttcgacattc ccctctatcg atcatctctt ctttgtggtc
    10681 atttttattt ttggttttct ttcaacttac caagttcaat cttaatacaa cgatccatta
    10741 cgatatctgt acgtcctgct tttcttagca tctcagctgc ttctttgttt tcaatcccta
    10801 attgtgccca gaacacttta gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa
    10861 attcactccg tctaaataca tctacgatat cgattggatc atcgatttct gtcaaattcg
    10921 catagacttt ctcgcctaga atttcttcac cagccaataa tggatttact ggtatgattt
    10981 tatatccatt gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct ggtttattgc
    11041 tcaagcctac gacggcaata ttttttgctt cttccaaata gttgaaaatc gtttcttgtg
    11101 ttggattttt tacagacatt tctgccaccc cttgcttttc tttttattcc atctattata
    11161 cttgtatctt tcattttttc aattgttcct gttacctatt catgctaaaa taaaggatag
    11221 ttttcaactt gaaagagggt aaaaaatgaa attgatctta ttacttattt tagcatatct
    11281 tttaggttct attccttccg gtgtctggat tggcaaattg tttttcaaaa aagatatccg
    11341 acaacacggg agcggtaata ctggaacgac caatacattt cgtgtattag gcaaaacagc
    11401 cgggattgtt gtcctgctta tggatatttt aaaaggtaca ttggctactt gtttgccgat
    11461 gatcttccac ctttctggga ttaaccctct ttggtttggg gtatgcgcca ttcttggcca
    11521 tacatttccg atttttgctg ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat
    11581 gcttttagga tttaatcctg tattttttgt ttattcttgt atcatcttta tcgttagttt
    11641 attttgtaca agtatggtga gtttgagcag tatgatcagt gcagtattga tcactttatc
    11701 tacgatcgtc cttccttatg tagctcctgt gattttggct aagcccaatt ggttactgac
    11761 gatcattgcc tttttagttt cttcctttat cttttatcgg cataaagaca atatcaagcg
    11821 tatcagaaac catactgaaa gtcgaatccc gtttggtttg aatagcagca agaaaaaatc
    11881 ctgatacttt ttcattccaa agagataaaa cgggagtgtg acaaatcgat gtcacactcc
    11941 cttattactt acaggatagc ttttttacac accttggtaa acggtgttcc ctgctaactt
    12001 ctcggtcaca acctcttact tcactgtgat ggaatatttt gtcttgaaga tttcatgggc
    12061 acctagttga ttgatcccta ctttttccat caagtttcca ctactgttca ccgtgtcagc
    12121 gattccgcac cacggttcga tacaaacgaa aggcgcttct tttggataag gagaccagat
    12181 ccctacataa ggcatatctt tatagcttaa agttacgcta tgggaagatt tttcacttcg
    12241 aatggcgtac gcatttagac cccttgtttc aaaaaccaag gcatcttgct caaaaagttc
    12301 acgattcaaa gccagattcg tatttgtctg acctaacgtc ttttgatcca tatcgatgaa
    12361 tgggcctttc aatggtaggc gtaatcttga tttcaatgga gaaaatgcca gatagaaatc
    12421 ttcaaatgac aatccttctt caagaggtac attgaaagca ggatgtccac cgatagaaaa
    12481 gaacatttct ttttcaccgg tatttgccac ttgataatga accgtgatgc cgtctcctcc
    12541 taattcatag ctgatcacta attcaaaatc aaatggatac ttttctaatg tctccggcgt
    12601 actccttaag atgaaactag ccatttcttg tccatgttca atgacttcaa aatccatatc
    12661 ccgagcaaat ccgtgttgcc ccatttgata tgttttccct tgataaacat actgatcatc
    12721 ttttaaacgt ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat acgctggatc
    12781 accttgccag atatactcta aaccatgttc ttttgatttc agactgatca attccgcacc
    12841 gtgttcagcg atttcggcga tcagctggtc attttgaata ataacagtca ttgccattcc
    12901 tccaacttcg acttactttt taaaatggga aggctggaac aaacatttct atctgcccca
    12961 gtccccttac ttctaataac tgtgttttgc taactcgtct ttaaaacggg aattcaatac
    13021 ttttaaataa gttcccttca tgcctaacga acgagattca atgattccag ctgattccaa
    13081 ttttcgtaaa gcattgacga tcacagaacg agtgatgccg atactatcgg caatattaga
    13141 agcagtcaga cgaccttcat ctccatccaa tgcttcaaag atagcttgta cagccttcaa
    13201 ttcgctataa gataatgtgt tgattgccat ttggacagcc gttgcactcc gtacatctgc
    13261 ttcgatgctt cgagacttct gataaaggat ctgcataccg acaaccgtag ctccatattc
    13321 tgctaagact agatcgtcat ctgcaaacga ttgttccata cgagataaga taatcgtacc
    13381 caaacgttcg ccagctccaa agatcggaac gacagtcgtc agaccaaatg gatatttctc
    13441 tcttgactct accggaaatg cagtaatatc actagtaatt gggatattag cttcagtttt
    13501 catcaactgt gccactgtat cagtataaga ttcaggaaat tgtttctcaa caaacattgt
    13561 tttgattcgt tcattgttca catcgtgacg ttcattataa cctagtaata ctccgtcact
    13621 tcctatgata taggtgttgc tgtcaagaat accacctagt gtgacagcca ttttatcgta
    13681 aggcaaatct gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg
    13741 ggttttcgtt agcaagtctg tcattgtcgt cctccgattt tttataaaat atagcgactt
    13801 aaatcttcat ttgccgcaat tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt
    13861 atttctccca tttgcatatc tggtgcttca aataatagat cttctagtag tttttccaaa
    13921 atggtatgca gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc gaaagcgatt
    13981 tgagcgatcc gttcaattgc ttcttttgta aacgtaactg acacattttc tgtaccgatc
    14041 aacgcgacgt attgttttac taaagcatta tttggttcgg tcagaatctt cacaaaatct
    14101 tcagctgtca aatcatctaa ttctacccgt atcgggaagc gtccttgaag ttctggaatt
    14161 agatcacttg gtttagacaa atggaaagct cctgaagcaa tgaataggac gtgatctgtt
    14221 tggatgatcc cgtattttgt gtttacttgt gaaccttcga caattggcaa tatgtctctt
    14281 tggacgcctt ctctggatac ttctccagat tgctgtgatt tagaagtgat cttatcgatt
    14341 tcatcgataa aaataattcc actttcttcc gctaatttga ttgcctcact atggatatca
    14401 gcatccttga ctaattttgc tgattcttcc ttgatcaata actctaaagc ttctttgact
    14461 gttaccgtcc gttccacttt tttcttcggt gtcaaagcac ctagagtttc atttaggtcg
    14521 atccccattt gctccaatcc attgttcatc attggtgcag gtttcttttg gtcttcaact
    14581 tcaatggtta cttcacgact atccagtaaa cccttttgta gttgttccaa gactgtacgg
    14641 cgattgattc gaatatcttc tgtcacttct tcttgcggtt cagcggtatt ttgctgacta
    14701 gcattaaaaa gattcatcat ttgttcaaat tgattagtgg attgtttctg ttcttttttg
    14761 ataccaggta ccaagatttt taccaaacgt ttttctgcat tttttttagc acttgtgtat
    14821 acacggctgt actgctcttt ttctacgatt tggatcgcat tttctactaa atcccgaacc
    14881 attgattcta catctcttcc gacataaccg acttcagtaa attttgttgc ttcgactttg
    14941 acaaatggtg cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt accgacccct
    15001 gtaggtccga tcatcagcat attttttggt gtaatatctt gttgcatttt ttcatctaac
    15061 tgcaaacggc gatagcggtt acgcaaagca acggcaacag atttttttgc tgcgtgctgt
    15121 cctacgatat attcatctaa ttcacttacg atttgtcttg gtgtttgttt taattcatac
    15181 atatctactc acctcttaca gttcttctac gataatatta tgattagtaa agatacagat
    15241 atctgcggca atatttaacg cattttctgc aatttctttc gcactcatgt cgttttgagc
    15301 atagtttttc attgctcggg ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa
    15361 gataccatca tctggtgtga taacttctcc tgtacctgaa acaagaagca tttctttatc
    15421 attcattacg atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc gccattcttg
    15481 agctaattcg acagctgcgc gttgtagatt cccgttatat tcgtttagtt tgccttcaaa
    15541 tttttcttct aaagtaaacg catctgcaac acttccggcg aaacctacaa caacttctcc
    15601 attgtagata cgtcgtactt tctttgcagt gcctttcatg acaacagatt cacccattgt
    15661 tacttggcca tctccagcca tagcaaattt cccgtctttt tcgacggcac agatagtggt
    15721 tgaatgaaat ggtgattcga ccatgtttgt ttcctcctaa atttgttctt ttccttattt
    15781 ttacgctctt ggatggaaag aacgatagtt tttttgtaaa ctttcttttg ttacatgggc
    15841 atagatctgg gtagtcgata aattggcatg tcctagcaat tcttggactg tccgcaaatc
    15901 tgccccatta tttaataaat gagtagcaaa tgtatggcgc agcatgtgag gatggatatc
    15961 actatccaaa ctgctttttt tgatcatttg attcaagaca tattcaattc cagtaggcgt
    16021 gattggtttt ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat attttgtcat
    16081 caatacctgg cggccttccc tgaaatattc ttgcagagca tcttgagcaa aagaacctag
    16141 aggagcatat ctctctttgt tccctttacc atggatcaac atgacctctg aatcaaaatc
    16201 aatgtctgat accgacaaac tgctacactc acttaaacgg atgcctgacc cgtaaaggac
    16261 ttccagtaaa gcgcggttcc tcaaatccaa aggactatct cctgctacgc tgtcaaaaag
    16321 tgcttgcatt tcattttcat aaaaaaaacg tggcagtctc aaatttttct ttttgagatg
    16381 aacgtaagaa aatggatttt cttttatcac ttcttctttg agaagatact gataaaacga
    16441 acgtaggctg gctatcttgc gactgataga atttctgcta tagctaggac gatgatcagc
    16501 taatgcactc aaatagacac gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc
    16561 agattcctcc aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt ctttcgtttt
    16621 ttctgaatat cctctttcga caatcaaata acgcaaaaat ttttgttccc acgtggcttc
    16681 tgtcattttc tcaccttcct tttgtcccag agaataattt agcacagcgt ttataaaaag
    16741 acaaataaat tgtcagaatt taaccaatat ttctaagttt gttcacaatt ggcgcaattc
    16801 ctcacatttt gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga gactatccgt
    16861 ctcaccctct tcatggagtt tccttactca tttgggattg cagttttttc taattctgtt
    16921 tttattgcct cattagcttt taaagctcgt tgtgcaattg cttcatatct tgcttttttg
    16981 tctcgaatgc gctctggcag ttcaggaaac agaccatagt tggcattcat cggttggaaa
    17041 tgttttcctt ccgcatgagt aatgtagtaa gccatacttc caatagctgt ttcttgtgga
    17101 aactcgactg gttccatttc ttttgctaag cgtgcggcgt tgattccagc aagtagtccg
    17161 ctcgctgcac tttcaacata gccttctaca ccagtcatct gacctgcaaa gaacagatct
    17221 tcgcgtttcg ttgattggta agtcggtttc agcaattctg gcgaattcat aaagctgttt
    17281 cggtgcatca caccatagcg aacgaattca gcattttcta acccaggaat catccggaaa
    17341 acacgttttt gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa
    17401 gctgcagcat tgtcttgacg cagctggata acagcataag gacgtttgcc agttttagga
    17461 tcttccaatc caactggttt cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc
    17521 attacttcaa ttggcataca tccttcaaaa tatttttctt tctcaaaaga tttaagttct
    17581 gccacttcgg cattgattag tgcctcatgg aaagctttga attcctcttc tgtcatcgga
    17641 cagttcaagt aagctgcttc tcctttgtca tagcgggact tcaaatatac tttatccata
    17701 tcgatcgtag ctttgtctac gattggtgca gctgcatcat agaaataaaa accatctgaa
    17761 ccattgaatg ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc tgttgctact
    17821 acggtgattc cttccggtat ttcagtaatt tcttcgttta tcactgttac taatggatgc
    17881 tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca
    17941 ccagctggca ctgctgtttc atctgccgaa gtgattacta ccgaattcaa tcggcgcatt
    18001 tcttctttca ataaccctac tgcattagcc aaactgtttc ctcttaatga attggaacat
    18061 actagttcag caaaattact tgtgtgatgc gcctcagtcg atttgactgg acgcatttca
    18121 tataaacgga cagggactcc tgcttgtgcg atctgccaag cagcttcact tccggcaagc
    18181 cctgctccaa caacatttac cattttcaat gaaagaacct cttttctatt atttttggat
    18241 gtcttcttcg taatctccat ttacacagac tacttgttta ccgcctttga ctttcttctc
    18301 gaccagatat ccgtcacatt ttggacaatt tctgcccact ggtttatccc aagaagtgaa
    18361 ttcacattct gggaaacggg aacaaccata gaatagtctg tttttcttag attttcgttc
    18421 aatgacttgt ccttcatgac atactgggca agtcacaccg atttctttta cgattggttt
    18481 cgtattccga caatcaggaa aattactgca agcataaaac ttcccatatt tccctaattt
    18541 tatcaccatc ggatgtccac agacatcaca atcgaatcct gctggttcat ctttgatttg
    18601 aattttttcg attttttctt ccgcattcgt cagttctttt tcaaacggtt tatagaaacg
    18661 atcaaccact ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat ctccttccat
    18721 ttctgctgtg aaatggatat cgacgatttg agggaagaat tcacagatta atgaattgac
    18781 aatttctcct agttcagtcg gctcaaatct tttttgtgtc aattttacat agtagcgtct
    18841 ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt tttcttctag
    18901 tgcacgaatc aacgtagcct cactaaaacg tgctggtggt tgagtaaaat gttgttttgg
    18961 ttcaatatca acagattgga ctacatcgcc ttcttcaagt tctggaagaa tattttcttt
    19021 ctcttctttg ccgtcatctc taccttccac atacacttgc atgaatcctt tgaatttgat
    19081 ttttgaaccg ttcgcaatga agatgacacc attttgttct aatgtcactt tcattgtatc
    19141 taaaatagct ggtgtcatct gactagccac aaaacgtgac cagatcaatg tataaagttt
    19201 caattgatct ttatctaaat attgtttgag ttcatctggt gtacgaagca cactcgttgg
    19261 acgcacagct tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg
    19321 tctagaaaat tcttttccat acgtatcttc aataaatgct gcggcctctg cttttgctgt
    19381 atcggcaatc cgggtagaat cagtacgcat gtatgtaatc aagccgactg ttccttgttt
    19441 acccaaagtg attccttcat ataattgctg tgctaccatc atggtttttc ttgttcgaaa
    19501 gtttaactta cgggcagctt cttgttgcag gctacttgta gtgaaaggat tcgcagggtt
    19561 tcttttgcgc tctttctttt ctactttttt aacttcgaag tctgttccat caattctagc
    19621 agtgacttct tttacacttt gtgcatctgg taatttcttt ttcttaccgt cgattcccca
    19681 gaaatttgct ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc
    19741 ttctggaaca aattctcgga tctctttttc acggtcaata ataattttta aagcgacaga
    19801 ttggacacgt cctgcactta accctttctt gaccttacgc cagagaatag gactgatcga
    19861 ataaccaacg attctgtcta aggtacgacg agcttgctgt gcatctacta aatctacatc
    19921 gatcgttctt ggttccttaa aagctgcttt gactgcgtct tttgtgattt cattgaagac
    19981 cacccgattt ttttctttca gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc
    20041 ttctccttct ctatccgggt cacttgccaa gtaaactttt tgagcttttt ttgctgcggc
    20101 tttcaggctt ttgatgacat cgccttttcc gcgtatagaa atataatggg gttcataatt
    20161 gttttcaaaa tcgataccca ttttactttt cggtaaatct cttatatggc caacactggc
    20221 cattacttta tagtttcgtc caagatattt ctcaattgtc ttggcttttg caggtgattc
    20281 tacaatcact aaatatttat acgccattac tgaatggact cctttccatt gtcgtgttcc
    20341 tactattaaa agaggattca tacaaatcgt agctaatcat atccattcag aaaacgtttg
    20401 tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa ttctgccaag atatcttcac
    20461 ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg acatccggta gaatgttctg
    20521 ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag atccaatgct tgctgtgctg
    20581 ttatcagaga gccgcttttt ttcttggctt caaagacaca tgttccttta gacaaagctg
    20641 cgataatccg atttctcatt ggaaaacaat gctttttcgg gccggattga ggaggatatt
    20701 cactcaaaag aagatgatca tttgcaatca cctcttgcaa gtcacgattt tcagcaggat
    20761 agacttgctc tatccctgtt ccaatgactg cgattgtccg tccaccatga gagattgctg
    20821 cttggtgacc gtatgtatct atccctttcg cgcagccact gacgatcaca tatccagctt
    20881 ctatcactgg ttttagtaaa taataaactg tttttttggc taaagcagaa gtgtgtcttc
    20941 caccaacaac gcttaacatg ttacaagaaa gtaaagatag atcaccatta taaaacagta
    21001 caattggcgg ataggtcaag tgaagcagct gagggggata tttgggatca aagcaagtga
    21061 taaacgactg tttcgattgg attttgcccc agttagcgtg aatttcctgc caacttttgc
    21121 gaaacagctc ccggtgtttg gttattttac ctatcttaat gatgtcttca aatccgattt
    21181 ctttataagg atactgcatc atccagttag caagtgccca ttttccttga ctactgattc
    21241 cttttgtata agcaagtttc aataaatttt cttctatttg atacatcata aaaccgcctt
    21301 tcgtctctct ttctaataag atacgtaaaa gcggctgttt tttattgtaa atattttttt
    21361 acaggtgcaa aacttttacg atggattggc gtaattcctt gtcgttcaag tccttctaag
    21421 tgttcctttg ttccataacc tgcattatgc gagaaaccat atccaggata aagcacgtca
    21481 tattctttca tcaattggtc tcgatatact ttagcaatga tacttgcagc agcaatcgac
    21541 acagatcgtg catccccttt gattattttt tcctgctgaa taggcaaatc caaggtcatc
    21601 gcatcgatca acaaatagct tggtacaatg gacaattttt ctactgcttg gatcatcgct
    21661 aatttacttg cttcataaat attcacatca tcgattgtct gctgatcaac gatcccgatc
    21721 ccgattgcca gggctttttc ttgaacttgt tgataaagaa gttctcgctt tttctctgat
    21781 aattgtttgg agtcattcag ccctaatatg atctcatttt tcggcaagat aactgcagct
    21841 gccacgacag gtccagctaa tggtcctctt cctacttcat caatgccggc aatccattgg
    21901 tgaccttgtg cgtacccttg cttttcaaag caaaacattt cttcatattt ttccttaatt
    21961 aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt
    22021 ggatcatttc ttaatgattc tagtgtagga tcatctattc tctgtatgct tttcaatcgt
    22081 tctttgatac tttggatgga ttcagtcatc acttttttgc tccatttctt cgcaacgatc
    22141 cagtgtgtat cgtccaagtt ttccttgacg aatatcatgc acgactaatt cgctgccacg
    22201 atcatagtct tctttatacc cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa
    22261 tttttcttct tgctcgattg ataaaccata acgatcgctg attcttcctg aataatatcg
    22321 ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa tggacaagct ggtctttgat
    22381 cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct tcaaatttgg gccataaaat
    22441 ccctggtgtg tctaacaatt ccaactcatt acctaaacga agccactgct gaccttttgt
    22501 tacgccaggc ttatttcctg tctgtgcaat tttcttgcct actagtcgat tcatcaacgt
    22561 agatttccca acatttggaa tacctaagac cattgcccga atagcgcgag gcttgatccc
    22621 ccgagcagca tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc
    22681 tttaattcct ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa
    22741 atattcttgc cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac
    22801 aaggcgaggt ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa
    22861 tcgtgcgtct accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg
    22921 tcgggctttt gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt
    22981 cataccggaa tattccccgg ctttacttgt ttttttcttt ttcaatataa attcactttt
    23041 ttattataaa gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca
    23101 catcttcact tttttgatga tgcaaatttt tattttggtt tctatatatg aatctaaatt
    23161 tttgacagcg tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca
    23221 aatgaatagt atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt
    23281 ttatgaaaag ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat
    23341 ggtatgggat gacacttttt ggataatgct tctctctcat gatttttata aaaaatttat
    23401 aaaagataaa gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt
    23461 tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt
    23521 ccacgaagta gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct
    23581 agacggaaat acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt
    23641 tttttataac aaaaaaacaa aggcggatga tacgataagt attctccgcc tctatttttt
    23701 ttattcgtac aatctctttt ttacagttta taaaagcagt cttctcaccc tttttttaat
    23761 tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg attttatggt
    23821 atccttttat tattaactga caaaggagag aatattttga acactttgat tgcaaaagac
    23881 gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct tttattagcc
    23941 cttgatgtca tcttatacaa gatagcgatt ggtcctgctt attttcaaat cacattcgga
    24001 tttttaagta tggcgcttat gggttatcta tatggaccga tctgggctgg tttgcttgaa
    24061 atgttgtcga acactttaaa tttcacgata tttggttcag gaacgtttca agctgctttt
    24121 cttatcacgg ctttccttgg tggatttatt aatggcttat ttttatatca taaaaagata
    24181 aacctgcctt ccgtcatcgc tgcccaatta ttgattatga tcccagtcag cctttttatc
    24241 aattcttggc tgatgtctgt tttgtttggt gcagatttca aagtaatttt tctaggtaga
    24301 ttcatcagga atatgatcca aatccctatt caaataatgg ttgtttattc atttatcaaa
    24361 actttagagg ctagaaaagt gtttaaacga gctgtttgat tttacgtact aaggtagtaa
    24421 actgattttg gattaaacaa taaagcgtgc agtctcaaca gactgcacgc tttattgttt
    24481 ctcactggat ccattttatt tcatcaagtg gataatagac aaatcttgct ttacctagaa
    24541 tatcagtttt attgatcgtg ccaaacgaac gactatcttt tgaaactctt cgattatctc
    24601 caagtacaaa ataattatct tccggaagct tggattgtcc cgttagctct tgtagtgtaa
    24661 agtccgtcgt ataggatgca tttgcatgat ctgatttcag gtttttagtt agataaggtt
    24721 ccttaactac tttcccattg atcttcaatt gatcattttg gtagctgaca ttttctcctg
    24781 gaagaccgat tactcgtttg atataaatcg tgccatctgc taattgaaaa acaacaatat
    24841 caaatcgtct gatctccgaa aatttttcca tcacaaccat atcaccttgc ttcaacacgt
    24901 tttccattga attgccttcc actggtaccg ggatgaataa aaatccccgc aacatgaacg
    24961 caaggaatac agaaacaagt aagtatttga atacaagcca gaagcgatta atatagcgtt
    25021 gtttttttgt caatctctat caatccatcc ctcttttttt cattctttct ttattataag
    25081 aaaaatatca aaaaaatgcg actagatagc tgaaataaaa ataattcttt caattaggct
    25141 attggacaaa aaactttttt gatcataaag tacaaaacta tctcttatat gtcatgatta
    25201 ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca
    25261 ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg
    25321 tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt
    25381 gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa
    25441 aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt
    25501 gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct
    25561 gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt
    25621 cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga
    25681 aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca
    25741 atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt
    25801 gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac
    25861 caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc
    25921 gttccattat gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc
    25981 ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag
    26041 gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct
    26101 gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga
    26161 tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc
    26221 acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca
    26281 tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt
    26341 acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca
    26401 ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt
    26461 gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt
    26521 tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc
    26581 ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat
    26641 acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg
    26701 tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat
    26761 ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc
    26821 tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa
    26881 gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc
    26941 ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa
    27001 atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt
    27061 ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg
    27121 ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg
    27181 tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta
    27241 agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat
    27301 tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat
    27361 ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat
    27421 ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag
    27481 cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat
    27541 gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa
    27601 aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata
    27661 aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa
    27721 tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc
    27781 gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct
    27841 tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct
    27901 ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta
    27961 gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta
    28021 gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat
    28081 attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct
    28141 actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg
    28201 tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt
    28261 gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg
    28321 tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg
    28381 ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca
    28441 tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca
    28501 tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt
    28561 attttctctc tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca
    28621 atttctattt ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg
    28681 agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt
    28741 taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt
    28801 agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc
    28861 gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc
    28921 aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta
    28981 tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat
    29041 ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag
    29101 agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc
    29161 tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct
    29221 ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt
    29281 ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac
    29341 ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag tctatccata
    29401 gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata
    29461 cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat
    29521 cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg
    29581 gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc
    29641 tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact
    29701 atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg
    29761 acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt
    29821 atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg
    29881 gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc
    29941 atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt
    30001 ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta
    30061 aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga
    30121 tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat
    30181 gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca
    30241 catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa
    30301 acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa
    30361 cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat
    30421 cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa
    30481 gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc
    30541 tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag
    30601 tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc
    30661 aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa
    30721 gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt
    30781 tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt
    30841 cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca
    30901 tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc
    30961 ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt
    31021 atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat
    31081 ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc
    31141 aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg
    31201 cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa
    31261 tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac
    31321 agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc
    31381 attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa
    31441 taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg
    31501 aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc
    31561 ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt
    31621 ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag
    31681 gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt
    31741 aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc
    31801 atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc
    31861 taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc
    31921 gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca
    31981 agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc
    32041 acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc
    32101 aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac
    32161 catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata
    32221 atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg
    32281 cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc
    32341 actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc
    32401 tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt
    32461 ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt
    32521 tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag
    32581 ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc
    32641 gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc
    32701 gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata
    32761 agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata
    32821 ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt
    32881 ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac
    32941 ttgaaaattt ttaccttttt attttattgg actcgttttt tttgactttg ctcgaaacgc
    33001 aaatcggaaa gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat
    33061 attcctgcga tagctgttcg cagaaaacgt cgtgattttt tcagcaactt cgtattcatc
    33121 aatgattttt ctttcaagat aataacctct tagcattttt ttcgtaaaca gactatcttg
    33181 ctggatcatc cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg
    33241 ttgctggata aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact
    33301 ttcaataatc aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc
    33361 actaataaat gtctgacgat cagcatatct tctttgccaa aacattttcg gatggagaac
    33421 gattcgcatt aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct
    33481 tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga
    33541 aagcttgccc gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc
    33601 ttgtatttct tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg
    33661 tatccgttct tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg
    33721 atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc
    33781 attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc
    33841 tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta
    33901 aaacatcata ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc
    33961 ctttacaccg ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact
    34021 gccatctttg aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac
    34081 agagattaat acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac
    34141 tggcgagaac acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt
    34201 aaaagcaata gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga
    34261 gattttccta aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga
    34321 tacatcaaat gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag
    34381 ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa
    34441 ccagacaagc aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc
    34501 cgcgtttact gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt
    34561 aacaccagat gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg
    34621 gatattcact cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc
    34681 tccaaaagca aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc
    34741 taccccgaca agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt
    34801 tttgagaatc gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat
    34861 cataactaag aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa
    34921 taccactcca aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc
    34981 tatgttcgat aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt
    35041 ccatcccctt tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa
    35101 aattttggtt tcgactactt caacctgtgt ttggttcccc tctaattcac actgtacacg
    35161 ttttgactcc aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat
    35221 atttctttta cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata
    35281 gcgatcatgc aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta
    35341 tacacaaact tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc
    35401 atcgtttatt gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat
    35461 agtatcagat atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa ggatgtttac
    35521 tttcccattc ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa
    35581 atcttgtcac agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta
    35641 ataagacaca tctctttcac agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt
    35701 ccccgtatac atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt
    35761 tttcgctgat ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat
    35821 atgggaagta cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat
    35881 cttcgtattt caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat
    35941 gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga
    36001 tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa
    36061 gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta
    36121 gcgctgtcat ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact
    36181 acctcagttt agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac
    36241 gtatctgccc actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa
    36301 tcaatttttc tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt
    36361 ctacgacggc tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc
    36421 cagtttcttc tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta
    36481 aacgatcata ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca
    36541 attcttctac ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag
    36601 ctccagtttc tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt
    36661 tagaacactt ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc
    36721 tgatcaataa tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa
    36781 aacgtaaaag tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt
    36841 caataaatgc atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca
    36901 catttatacg ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga
    36961 aggtttcttc ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc
    37021 gtaaggcatc ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt
    37081 ctagatcttg cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg
    37141 cattgatcgt aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat
    37201 gatctggtct acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt
    37261 gttcatcatt ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg
    37321 gaaaaagctg tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg
    37381 gctggttcag caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat
    37441 tctctaattt tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt
    37501 tcatttttcc aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt
    37561 gactgtttga caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact
    37621 cgtccttacg ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa
    37681 aaacttcagt taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat
    37741 caccaagttc ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat
    37801 aagtgtggtt gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag
    37861 ctaatggaga gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca
    37921 tactgtataa agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact
    37981 ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga
    38041 aggaatttct aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt
    38101 caaacgaaaa acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt
    38161 tggtcttgtt tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac
    38221 cttgatctta agagctttat ttggtattga tccagcttat acgactttat taatcaacat
    38281 cccattgatt ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct
    38341 aggtacactt tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa
    38401 tttacagcat gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag
    38461 cgggatcgtc taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt
    38521 agaacaaaat tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt
    38581 acttgcttca ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta
    38641 tgttttcagt cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact
    38701 tgtcgtctcc aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg
    38761 tgtgactttc ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat
    38821 ggtcgttagc tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa
    38881 agctttcctt tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa
    38941 gccacagaca agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa
    39001 agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt
    39061 tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt
    39121 catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag
    39181 cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt
    39241 cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag
    39301 atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt
    39361 tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca
    39421 aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg
    39481 caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat
    39541 aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta
    39601 aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc
    39661 taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc
    39721 ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc
    39781 tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc
    39841 ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc
    39901 catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact
    39961 taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc
    40021 ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc
    40081 gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat
    40141 ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat gcggattaac
    40201 ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt
    40261 aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg
    40321 tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat
    40381 cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc
    40441 ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc
    40501 atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat
    40561 aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc
    40621 cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc
    40681 taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg
    40741 ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc
    40801 tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc
    40861 tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac
    40921 tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt
    40981 atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa
    41041 aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc
    41101 caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga
    41161 gattccacac gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat
    41221 ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt
    41281 ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg
    41341 ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa
    41401 tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc
    41461 tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat
    41521 ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata
    41581 cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat
    41641 ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt
    41701 tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa
    41761 tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat
    41821 cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct
    41881 gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa
    41941 gttccatacg catccctcct cttcttattt ttattttacc attagtaagc aaattctaga
    42001 aaagataaga gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca
    42061 tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct
    42121 gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt
    42181 cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg
    42241 gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa
    42301 cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa
    42361 tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg
    42421 aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa
    42481 gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag
    42541 aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa
    42601 ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc
    42661 tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat
    42721 aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg
    42781 ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt
    42841 gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg
    42901 attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt
    42961 ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac
    43021 tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg
    43081 ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat
    43141 aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat
    43201 cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc
    43261 acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc
    43321 tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc
    43381 tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat
    43441 caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc
    43501 ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc
    43561 tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata
    43621 gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt
    43681 acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct
    43741 gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg
    43801 atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg
    43861 acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac
    43921 aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg
    43981 gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc
    44041 acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat
    44101 ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct
    44161 gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt
    44221 cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt
    44281 cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt
    44341 ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca
    44401 ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa
    44461 tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc
    44521 ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt
    44581 ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat
    44641 tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca cttggttttg
    44701 ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga
    44761 caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga
    44821 tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg
    44881 ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc
    44941 ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac
    45001 cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga
    45061 tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac
    45121 ggcgttttga aagtagatag ctgccgtttt ctttatcttt gccgatcgta ctgataacga
    45181 ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg
    45241 ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt
    45301 gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta
    45361 cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca
    45421 taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt
    45481 gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc
    45541 attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc
    45601 tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg
    45661 cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt
    45721 cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc
    45781 acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt
    45841 cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc
    45901 aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc
    45961 tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc
    46021 ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga
    46081 aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc
    46141 tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga
    46201 agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa
    46261 aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat
    46321 aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg
    46381 ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc
    46441 tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc
    46501 ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt
    46561 atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt
    46621 tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga
    46681 agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg
    46741 aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt
    46801 cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg
    46861 ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac
    46921 tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta
    46981 aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgttcc ccaatgaagt
    47041 tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata
    47101 tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt
    47161 ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct
    47221 ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc
    47281 tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa
    47341 agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca
    47401 tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg
    47461 atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata
    47521 tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt
    47581 tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat
    47641 ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca
    47701 caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt
    47761 tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct
    47821 tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc
    47881 gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga
    47941 tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat
    48001 aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga
    48061 ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc
    48121 gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact
    48181 tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac
    48241 cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct
    48301 tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca
    48361 aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat
    48421 ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg
    48481 ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat
    48541 ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt
    48601 tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat
    48661 ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc
    48721 gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct
    48781 ttgttgcaga taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc
    48841 tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc
    48901 cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg
    48961 gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag
    49021 atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg
    49081 ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg
    49141 atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc
    49201 gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc
    49261 tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt
    49321 cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga
    49381 aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg
    49441 tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa
    49501 gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag
    49561 gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa
    49621 tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa
    49681 cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa
    49741 atcccgactt attttgaaag attcatttgc tatctatagt taattgacta gttttttcac
    49801 ggaccctata ggacccattt atattatttc aactttttca gccagttttc caatgtattg
    49861 acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca
    49921 ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc
    49981 ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc
    50041 gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt
    50101 gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt
    50161 gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct
    50221 ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata
    50281 gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct
    50341 acgagagcac caggataaaa aataagtaat ggagactttc catgattctt tttctctcct
    50401 tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac
    50461 gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa
    50521 atgatgacta agccaattag tattcgtcta aatattttca ctttctccca ccttttctat
    50581 ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat
    50641 gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt
    50701 tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa atttccattg
    50761 ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat
    50821 cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc
    50881 ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt
    50941 tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag
    51001 tcaacccata tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg
    51061 taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg
    51121 caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac
    51181 cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt
    51241 ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt
    51301 ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt
    51361 ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa
    51421 caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt
    51481 aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc
    51541 cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt
    51601 catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag
    51661 ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa
    51721 ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc
    51781 ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct
    51841 ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga
    51901 tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg
    51961 aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt
    52021 gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa
    52081 agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc
    52141 tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat
    52201 ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat
    52261 gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac
    52321 acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac
    52381 atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt
    52441 tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa
    52501 ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat
    52561 caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa
    52621 tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat
    52681 aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc
    52741 accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac
    52801 gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag
    52861 catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga
    52921 ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat
    52981 accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg
    53041 acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa
    53101 tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat
    53161 agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc
    53221 aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac
    53281 ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat
    53341 gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc
    53401 gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt
    53461 cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa
    53521 tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag
    53581 ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc
    53641 ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac
    53701 actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa
    53761 taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt
    53821 cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga
    53881 gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt
    53941 tccctattcc ctcctctttt ttcaggtttt taaagctttc ttctttttca ttgtagcgtt
    54001 tccttttttt tataaacaaa cattttcgtc ttttaaaagg agaagaattt cacatttgca
    54061 aagaattgat tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg
    54121 tttacattat tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat
    54181 ctttttgata tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt
    54241 aaccatggaa gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg
    54301 atcaagttga aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc
    54361 tgtgttgcgt tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg
    54421 atactcataa aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt
    54481 cctttatgct gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt
    54541 gataagaaac tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga
    54601 tgtaatactg aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg
    54661 ctgatcaata ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc
    54721 ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt
    54781 ttctgtacac gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact
    54841 ttttgacgtt tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt
    54901 atttgatcaa gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat
    54961 cggaatttca ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt
    55021 cgggaatttc accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt
    55081 atacatatct attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg
    55141 agtggttcag caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta
    55201 ggccttgtaa agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa
    55261 ctgcttgaag catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga
    55321 tactgtggtt tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc
    55381 gtccttcgat ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt
    55441 ttacctttgc aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa
    55501 aatctccatc atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag
    55561 gatagatacg ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat
    55621 ctgcaacagc agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt
    55681 gataaattgg gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt
    55741 cccttacaac ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc
    55801 gacgagaagt gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc
    55861 tcctttattc attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc
    55921 catttcttgt gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta
    55981 gccagttgtt ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat
    56041 cagtttatgg gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc
    56101 gatttcagct cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc
    56161 ttgagataat gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt
    56221 tttagtcgtt aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt
    56281 ttgagtgtaa gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt
    56341 taacagtccg cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc
    56401 ttcaagttca cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg
    56461 attcatagtt ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag
    56521 ccataaaagt agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac
    56581 agccatgcgc atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg
    56641 tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt
    56701 tctgcatggc acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag
    56761 catccgctaa cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg
    56821 ctttttctgt cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga
    56881 ccaattcttc tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga
    56941 caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc
    57001 ctgcaacagc tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga
    57061 tcgtagcgat cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca
    57121 gatcgagagg accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc
    57181 tttcttcttt ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg
    57241 gatggagaca tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc
    57301 accgcgataa gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca
    57361 gtaaatacca ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc
    57421 ataatcgtct tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag
    57481 tttcttatgg aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt
    57541 taacatcttc tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt
    57601 cattcttttc ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa
    57661 caattcctgt actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg
    57721 ccgcagcgta tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag
    57781 atttttccag attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat
    57841 ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg
    57901 gatcgcataa tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac
    57961 cagtccaaga gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat
    58021 cccagtagca tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt
    58081 atcaggtgct tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg
    58141 tgctttcttc ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg
    58201 taaaaactga tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt
    58261 tttttcttca tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca
    58321 tgaagcaatt ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct
    58381 ttgtcgtgta ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat
    58441 ttgctctttc ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct
    58501 gatagtcttt ttccataatc tgagcaaaga aaaagccgtc tagacactca gacggactgg
    58561 tttctttttt ctttttcata gcattcctga cagattccat ggaacgttag ccgatgatct
    58621 tttactaaaa aatgataacg ttcttctact acttgctcca cttcaagtaa aagatcctct
    58681 tctacttcct cgatcgtgcc gcagttcaag catagtaaat gatgatggaa atgtttggcg
    58741 ccttcttttc tcaaatcata acgggctaat ccatcattaa aacttacttt atcgacaatt
    58801 ttcaattctg tcaagatttc aagggttcga taaacagtag caagtccaat ttccggactc
    58861 ttttttttca ctaaaaagaa gatttcttct gcagataaat gctctttctc attttccaaa
    58921 agcacaagca gagtagcttc tctttgcggt gtcagcttaa accccgcttc atgtagctgc
    58981 tgttttgttt ttttcatcgc cgaaatagca ttcatgacca tttactcaac cccaacaatt
    59041 tataataatt acaaataaaa aaagatataa cactatgtta attaaaatta ttataagcta
    59101 gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt
    59161 tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt
    59221 tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt
    59281 gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt
    59341 catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc
    59401 gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc
    59461 caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc
    59521 ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt
    59581 tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca
    59641 agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga
    59701 caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag
    59761 taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat
    59821 tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac
    59881 cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat
    59941 tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc
    60001 tctatagtga aaggttttct taatacattt ctttgctatc ataacataat gttatctact
    60061 tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat
    60121 actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct
    60181 aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt
    60241 aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat
    60301 atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt
    60361 caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc
    60421 acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct
    60481 gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg
    60541 cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt
    60601 gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct
    60661 ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat
    60721 ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg
    60781 aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt
    60841 cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt
    60901 tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat
    60961 gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct
    61021 tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac
    61081 tggataaatc attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt
    61141 gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct
    61201 gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc
    61261 agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt
    61321 ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt
    61381 cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct
    61441 cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag
    61501 catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta
    61561 tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag
    61621 taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt
    61681 tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta
    61741 ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt
    61801 cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat
    61861 tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt
    61921 cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt
    61981 tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt
    62041 cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat
    62101 cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat
    62161 ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac
    62221 cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt
    62281 tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc
    62341 tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct
    62401 tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa
    62461 ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt
    62521 ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac
    62581 gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt ctcgtccggc
    62641 catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta
    62701 gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg
    62761 cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt
    62821 caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat
    62881 ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac
    62941 gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac
    63001 gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg
    63061 aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc
    63121 cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta
    63181 ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga
    63241 ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt
    63301 tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg
    63361 tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat
    63421 tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat
    63481 ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc
    63541 gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg
    63601 ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt
    63661 cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct
    63721 ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat
    63781 cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc
    63841 gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag
    63901 gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc
    63961 atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa
    64021 tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg
    64081 tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg
    64141 ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa
    64201 ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag
    64261 ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc
    64321 ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc
    64381 ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct tttctggaac
    64441 gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt
    64501 tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc
    64561 catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat
    64621 accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt
    64681 tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag
    64741 ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc
    64801 aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac
    64861 tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg
    64921 ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc
    64981 attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg
    65041 caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc
    65101 cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat tttggacagc
    65161 tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac
    65221 cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc
    65281 tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt
    65341 acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac
    65401 atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg
    65461 aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag
    65521 aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta
    65581 aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac
    65641 atttctatct cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag
    65701 ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta
    65761 cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat
    65821 atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag
    65881 cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt
    65941 cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta
    66001 caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt
    66061 ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa
    66121 tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg
    66181 ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt
    66241 tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca
    66301 ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat
    66361 ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc
    66421 cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa
    66481 ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga
    66541 catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat
    66601 tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt
    66661 gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg
    66721 gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat
    66781 tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg
    66841 ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag
    66901 aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag
    66961 cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga
    67021 tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag
    67081 taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat
    67141 acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa
    67201 tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa
    67261 taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc
    67321 ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt
    67381 cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg
    67441 cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca
    67501 attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg
    67561 cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt
    67621 ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga
    67681 tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta
    67741 tttcttgata aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg
    67801 aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg
    67861 tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat
    67921 tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct
    67981 ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca
    68041 agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa
    68101 aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt
    68161 tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa
    68221 agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc
    68281 ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt
    68341 gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg
    68401 atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat
    68461 cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc
    68521 aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga
    68581 gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca
    68641 gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata
    68701 catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa
    68761 aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca
    68821 ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca
    68881 aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac
    68941 attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc
    69001 attttttgcc ttttttccac tatttgcgta ctccttcttt catttaattt tattcatgat
    69061 tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt
    69121 cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt
    69181 gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag
    69241 tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg
    69301 attttttaca taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa
    69361 atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc
    69421 aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg
    69481 catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc
    69541 gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac
    69601 tcgttttacc gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag
    69661 aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc
    69721 tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc
    69781 ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg
    69841 aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact
    69901 ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag
    69961 cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga
    70021 cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta
    70081 tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca
    70141 gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt
    70201 tcttggattt agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa
    70261 agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc
    70321 cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag
    70381 tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa
    70441 tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa
    70501 gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa
    70561 agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca
    70621 atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca
    70681 tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg
    70741 tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag
    70801 aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct
    70861 ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga
    70921 atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt
    70981 tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca
    71041 gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt
    71101 acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat
    71161 attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt
    71221 tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag
    71281 tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg
    71341 aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa
    71401 tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt
    71461 ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct
    71521 actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt
    71581 ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg
    71641 tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag
    71701 cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc
    71761 aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac
    71821 gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt
    71881 tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc
    71941 ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga
    72001 tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc
    72061 tgccggattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa
    72121 agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg
    72181 ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc
    72241 tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata
    72301 agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct
    72361 tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag
    72421 caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa
    72481 caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg
    72541 aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc
    72601 gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat
    72661 tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa
    72721 aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg
    72781 cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca
    72841 cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg
    72901 gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat
    72961 ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac
    73021 aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat
    73081 taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta
    73141 gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg
    73201 agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac
    73261 tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt
    73321 tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat
    73381 cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt
    73441 ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat
    73501 tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac
    73561 gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc
    73621 agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc
    73681 ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc
    73741 tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac
    73801 gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt
    73861 tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg
    73921 aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac
    73981 gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc
    74041 atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc
    74101 ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa
    74161 taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac
    74221 tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc
    74281 acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg
    74341 tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc
    74401 gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc
    74461 acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt
    74521 gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat
    74581 cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa
    74641 acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt
    74701 tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct
    74761 tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa
    74821 aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta
    74881 actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa
    74941 caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat
    75001 gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc
    75061 ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat
    75121 gtcagctacc caagcagata acggagagaa attcacgact tatacctaca agcaagcggc
    75181 ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt
    75241 aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga
    75301 agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat
    75361 ggtcttaaaa ttttattaga taaaa
//
LOCUS       DADVXN010000003        62415 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.003, whole genome shotgun sequence.
ACCESSION   DADVXN010000003 DADVXN010000000
VERSION     DADVXN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 62415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.003"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            281..601
                     /locus_tag="KB942_000149"
     CDS             281..601
                     /locus_tag="KB942_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBB6850607.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            755..1660
                     /locus_tag="KB942_000150"
     CDS             755..1660
                     /locus_tag="KB942_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6850608.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1657..2430
                     /locus_tag="KB942_000151"
     CDS             1657..2430
                     /locus_tag="KB942_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6850609.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2611..4035)
                     /locus_tag="KB942_000152"
     CDS             complement(2611..4035)
                     /locus_tag="KB942_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6850610.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4246..4692
                     /gene="nrdI"
                     /locus_tag="KB942_000153"
     CDS             4246..4692
                     /gene="nrdI"
                     /locus_tag="KB942_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB6850611.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4757..6739)
                     /locus_tag="KB942_000154"
     CDS             complement(4757..6739)
                     /locus_tag="KB942_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6850612.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6753..7649)
                     /gene="murQ"
                     /locus_tag="KB942_000155"
     CDS             complement(6753..7649)
                     /gene="murQ"
                     /locus_tag="KB942_000155"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB6850613.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7652..8713)
                     /locus_tag="KB942_000156"
     CDS             complement(7652..8713)
                     /locus_tag="KB942_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6850614.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8924..9490)
                     /locus_tag="KB942_000157"
     CDS             complement(8924..9490)
                     /locus_tag="KB942_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6850615.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9518..11020)
                     /gene="eat(A)"
                     /locus_tag="KB942_000158"
     CDS             complement(9518..11020)
                     /gene="eat(A)"
                     /locus_tag="KB942_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6850616.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11278..12399)
                     /locus_tag="KB942_000159"
     CDS             complement(11278..12399)
                     /locus_tag="KB942_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6850617.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12525..13466
                     /locus_tag="KB942_000160"
     CDS             12525..13466
                     /locus_tag="KB942_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6850618.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13616..14512)
                     /locus_tag="KB942_000161"
     CDS             complement(13616..14512)
                     /locus_tag="KB942_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6850619.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14781..16121
                     /locus_tag="KB942_000162"
     CDS             14781..16121
                     /locus_tag="KB942_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6850620.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16339..16782
                     /locus_tag="KB942_000163"
     CDS             16339..16782
                     /locus_tag="KB942_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6850621.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17286..18704)
                     /gene="nhaC"
                     /locus_tag="KB942_000164"
     CDS             complement(17286..18704)
                     /gene="nhaC"
                     /locus_tag="KB942_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6850622.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19107..20330)
                     /locus_tag="KB942_000165"
     CDS             complement(19107..20330)
                     /locus_tag="KB942_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBB6850623.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20361..21200)
                     /locus_tag="KB942_000166"
     CDS             complement(20361..21200)
                     /locus_tag="KB942_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6850624.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21217..21897)
                     /locus_tag="KB942_000167"
     CDS             complement(21217..21897)
                     /locus_tag="KB942_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850625.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21899..22972)
                     /locus_tag="KB942_000168"
     CDS             complement(21899..22972)
                     /locus_tag="KB942_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6850626.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23028..23266)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23309..23542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23619..24290)
                     /locus_tag="KB942_000169"
     CDS             complement(23619..24290)
                     /locus_tag="KB942_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6850627.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24377..27055)
                     /gene="mgtA"
                     /locus_tag="KB942_000170"
     CDS             complement(24377..27055)
                     /gene="mgtA"
                     /locus_tag="KB942_000170"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBB6850628.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27714..28151
                     /gene="ndk"
                     /locus_tag="KB942_000171"
     CDS             27714..28151
                     /gene="ndk"
                     /locus_tag="KB942_000171"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBB6850629.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28173..29039)
                     /locus_tag="KB942_000172"
     CDS             complement(28173..29039)
                     /locus_tag="KB942_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6850630.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29199..31292)
                     /locus_tag="KB942_000173"
     CDS             complement(29199..31292)
                     /locus_tag="KB942_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6850631.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32041..32664)
                     /locus_tag="KB942_000174"
     CDS             complement(32041..32664)
                     /locus_tag="KB942_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850632.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32823..34220)
                     /locus_tag="KB942_000175"
     CDS             complement(32823..34220)
                     /locus_tag="KB942_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6850633.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34252..35223)
                     /locus_tag="KB942_000176"
     CDS             complement(34252..35223)
                     /locus_tag="KB942_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6850634.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35597..36022
                     /locus_tag="KB942_000177"
     CDS             35597..36022
                     /locus_tag="KB942_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850635.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36510..37211)
                     /locus_tag="KB942_000178"
     CDS             complement(36510..37211)
                     /locus_tag="KB942_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6850636.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37227..37667)
                     /locus_tag="KB942_000179"
     CDS             complement(37227..37667)
                     /locus_tag="KB942_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6850637.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37667..38242)
                     /locus_tag="KB942_000180"
     CDS             complement(37667..38242)
                     /locus_tag="KB942_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBB6850638.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39090..39569)
                     /locus_tag="KB942_000181"
                     /pseudo
     CDS             complement(39090..39569)
                     /locus_tag="KB942_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39728..40429)
                     /locus_tag="KB942_000182"
     CDS             complement(39728..40429)
                     /locus_tag="KB942_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6850639.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40537..41946)
                     /locus_tag="KB942_000183"
     CDS             complement(40537..41946)
                     /locus_tag="KB942_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBB6850640.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41994..43859)
                     /locus_tag="KB942_000184"
     CDS             complement(41994..43859)
                     /locus_tag="KB942_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6850641.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43980..44830)
                     /locus_tag="KB942_000185"
                     /pseudo
     CDS             complement(43980..44830)
                     /locus_tag="KB942_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(45140..45853)
                     /locus_tag="KB942_000186"
     CDS             complement(45140..45853)
                     /locus_tag="KB942_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBB6850642.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45921..46247)
                     /locus_tag="KB942_000187"
     CDS             complement(45921..46247)
                     /locus_tag="KB942_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6850643.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46370..46597)
                     /locus_tag="KB942_000188"
     CDS             complement(46370..46597)
                     /locus_tag="KB942_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6850644.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46919..48172)
                     /locus_tag="KB942_000189"
     CDS             complement(46919..48172)
                     /locus_tag="KB942_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6850645.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48233..48973
                     /locus_tag="KB942_000190"
     CDS             48233..48973
                     /locus_tag="KB942_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6850646.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49059..49817)
                     /locus_tag="KB942_000191"
     CDS             complement(49059..49817)
                     /locus_tag="KB942_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6850647.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49814..50842)
                     /locus_tag="KB942_000192"
                     /pseudo
     CDS             complement(49814..50842)
                     /locus_tag="KB942_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50862..54029)
                     /locus_tag="KB942_000193"
     CDS             complement(50862..54029)
                     /locus_tag="KB942_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850648.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54253..55137)
                     /locus_tag="KB942_000194"
     CDS             complement(54253..55137)
                     /locus_tag="KB942_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6850649.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55232..55669)
                     /locus_tag="KB942_000195"
     CDS             complement(55232..55669)
                     /locus_tag="KB942_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850650.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55886..56797)
                     /locus_tag="KB942_000196"
     CDS             complement(55886..56797)
                     /locus_tag="KB942_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6850651.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57254..57682)
                     /locus_tag="KB942_000197"
     CDS             complement(57254..57682)
                     /locus_tag="KB942_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850652.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57710..58348)
                     /locus_tag="KB942_000198"
     CDS             complement(57710..58348)
                     /locus_tag="KB942_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850653.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58352..60334)
                     /locus_tag="KB942_000199"
     CDS             complement(58352..60334)
                     /locus_tag="KB942_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBB6850654.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60514..61179
                     /locus_tag="KB942_000200"
     CDS             60514..61179
                     /locus_tag="KB942_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850655.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        18165 a        12955 c        10608 g        20687 t
ORIGIN      
        1 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
       61 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
      121 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
      181 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
      241 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
      301 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
      361 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
      421 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
      481 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
      541 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
      601 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
      661 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
      721 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
      781 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
      841 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
      901 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
      961 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
     1021 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
     1081 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
     1141 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
     1201 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
     1261 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
     1321 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
     1381 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
     1441 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
     1501 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
     1561 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
     1621 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
     1681 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
     1741 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
     1801 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
     1861 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
     1921 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
     1981 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
     2041 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
     2101 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
     2161 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
     2221 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
     2281 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
     2341 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
     2401 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
     2461 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
     2521 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
     2581 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
     2641 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
     2701 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
     2761 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
     2821 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
     2881 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
     2941 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
     3001 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
     3061 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
     3121 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
     3181 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
     3241 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
     3301 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
     3361 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
     3421 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
     3481 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
     3541 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
     3601 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
     3661 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
     3721 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
     3781 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
     3841 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
     3901 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
     3961 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
     4021 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
     4081 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
     4141 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
     4201 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
     4261 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
     4321 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
     4381 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
     4441 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
     4501 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
     4561 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
     4621 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
     4681 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
     4741 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
     4801 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
     4861 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
     4921 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
     4981 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
     5041 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
     5101 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
     5161 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
     5221 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
     5281 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
     5341 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
     5401 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
     5461 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
     5521 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
     5581 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
     5641 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
     5701 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
     5761 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
     5821 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
     5881 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
     5941 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
     6001 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
     6061 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
     6121 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
     6181 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
     6241 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
     6301 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
     6361 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
     6421 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
     6481 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
     6541 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
     6601 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
     6661 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
     6721 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
     6781 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
     6841 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
     6901 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
     6961 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
     7021 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
     7081 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
     7141 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
     7201 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
     7261 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
     7321 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
     7381 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
     7441 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
     7501 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
     7561 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
     7621 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
     7681 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
     7741 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
     7801 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
     7861 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
     7921 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
     7981 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
     8041 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
     8101 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
     8161 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
     8221 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
     8281 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
     8341 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
     8401 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
     8461 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
     8521 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
     8581 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
     8641 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
     8701 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
     8761 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
     8821 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
     8881 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
     8941 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
     9001 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
     9061 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
     9121 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
     9181 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
     9241 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
     9301 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
     9361 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
     9421 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
     9481 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
     9541 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
     9601 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
     9661 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
     9721 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
     9781 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
     9841 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
     9901 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
     9961 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    10021 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    10081 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    10141 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    10201 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    10261 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    10321 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    10381 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    10441 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    10501 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    10561 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    10621 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    10681 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    10741 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    10801 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    10861 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    10921 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    10981 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    11041 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    11101 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    11161 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    11221 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    11281 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    11341 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    11401 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    11461 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    11521 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    11581 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    11641 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    11701 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    11761 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    11821 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    11881 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    11941 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    12001 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    12061 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    12121 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    12181 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    12241 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    12301 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    12361 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    12421 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    12481 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    12541 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    12601 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    12661 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    12721 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    12781 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    12841 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    12901 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    12961 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    13021 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    13081 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    13141 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    13201 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    13261 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    13321 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    13381 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    13441 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    13501 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    13561 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    13621 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    13681 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    13741 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    13801 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    13861 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    13921 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    13981 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    14041 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    14101 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    14161 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    14221 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    14281 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    14341 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    14401 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    14461 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    14521 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    14581 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    14641 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    14701 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    14761 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    14821 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    14881 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    14941 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    15001 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    15061 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    15121 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    15181 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    15241 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    15301 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    15361 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    15421 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    15481 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    15541 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    15601 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    15661 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    15721 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    15781 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    15841 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    15901 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    15961 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    16021 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    16081 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    16141 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    16201 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    16261 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    16321 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    16381 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    16441 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    16501 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    16561 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    16621 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    16681 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    16741 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    16801 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    16861 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    16921 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    16981 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    17041 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    17101 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    17161 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    17221 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    17281 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    17341 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    17401 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    17461 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    17521 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    17581 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    17641 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    17701 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    17761 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    17821 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    17881 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    17941 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    18001 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    18061 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    18121 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    18181 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    18241 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    18301 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    18361 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    18421 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    18481 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    18541 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    18601 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    18661 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    18721 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    18781 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    18841 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    18901 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    18961 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    19021 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    19081 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    19141 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    19201 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    19261 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    19321 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    19381 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    19441 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    19501 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    19561 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    19621 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    19681 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    19741 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    19801 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    19861 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    19921 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    19981 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    20041 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    20101 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    20161 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    20221 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    20281 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    20341 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    20401 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    20461 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    20521 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    20581 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    20641 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    20701 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    20761 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    20821 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    20881 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    20941 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    21001 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    21061 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    21121 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    21181 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    21241 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    21301 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    21361 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    21421 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    21481 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    21541 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    21601 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    21661 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    21721 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    21781 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    21841 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    21901 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    21961 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    22021 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    22081 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    22141 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    22201 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    22261 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    22321 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    22381 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    22441 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    22501 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    22561 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    22621 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    22681 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    22741 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    22801 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    22861 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    22921 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    22981 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    23041 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    23101 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    23161 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    23221 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    23281 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    23341 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    23401 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    23461 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    23521 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    23581 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    23641 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    23701 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    23761 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    23821 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    23881 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    23941 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    24001 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    24061 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    24121 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    24181 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    24241 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    24301 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    24361 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    24421 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    24481 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    24541 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    24601 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    24661 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    24721 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    24781 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    24841 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    24901 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    24961 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    25021 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    25081 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    25141 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    25201 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    25261 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    25321 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    25381 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    25441 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    25501 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    25561 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    25621 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    25681 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    25741 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    25801 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    25861 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    25921 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    25981 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    26041 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    26101 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    26161 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    26221 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    26281 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    26341 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    26401 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    26461 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    26521 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    26581 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    26641 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    26701 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    26761 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    26821 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    26881 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    26941 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    27001 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    27061 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    27121 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    27181 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    27241 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    27301 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    27361 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    27421 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    27481 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    27541 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    27601 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    27661 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    27721 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    27781 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    27841 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    27901 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    27961 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    28021 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    28081 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    28141 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    28201 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    28261 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    28321 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtga taagctccgt
    28381 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    28441 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    28501 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    28561 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    28621 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    28681 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    28741 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    28801 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    28861 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    28921 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    28981 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    29041 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    29101 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    29161 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    29221 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    29281 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    29341 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    29401 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    29461 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    29521 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    29581 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    29641 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    29701 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    29761 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    29821 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    29881 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    29941 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    30001 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    30061 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    30121 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    30181 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    30241 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    30301 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    30361 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    30421 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    30481 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    30541 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    30601 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    30661 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    30721 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    30781 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    30841 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    30901 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    30961 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    31021 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    31081 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    31141 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    31201 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    31261 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    31321 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    31381 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    31441 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    31501 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    31561 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    31621 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    31681 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    31741 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    31801 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    31861 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    31921 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    31981 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    32041 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    32101 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    32161 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    32221 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    32281 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    32341 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    32401 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    32461 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    32521 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    32581 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    32641 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    32701 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    32761 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    32821 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    32881 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    32941 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    33001 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    33061 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    33121 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    33181 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    33241 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    33301 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    33361 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    33421 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    33481 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    33541 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    33601 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    33661 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    33721 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    33781 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    33841 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    33901 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    33961 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    34021 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    34081 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    34141 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    34201 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    34261 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    34321 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    34381 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    34441 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    34501 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    34561 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    34621 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    34681 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    34741 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    34801 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    34861 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    34921 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    34981 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    35041 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    35101 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    35161 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    35221 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    35281 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    35341 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    35401 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    35461 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    35521 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    35581 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    35641 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    35701 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    35761 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    35821 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    35881 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    35941 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    36001 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    36061 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    36121 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    36181 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    36241 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    36301 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    36361 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    36421 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    36481 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    36541 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    36601 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    36661 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    36721 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    36781 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    36841 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    36901 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    36961 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    37021 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    37081 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    37141 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    37201 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    37261 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    37321 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    37381 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    37441 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    37501 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    37561 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    37621 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    37681 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    37741 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    37801 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    37861 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    37921 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    37981 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    38041 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    38101 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    38161 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    38221 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    38281 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
    38341 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
    38401 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
    38461 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
    38521 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
    38581 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
    38641 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
    38701 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
    38761 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
    38821 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
    38881 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
    38941 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
    39001 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
    39061 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
    39121 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
    39181 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
    39241 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
    39301 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
    39361 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
    39421 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
    39481 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
    39541 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
    39601 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
    39661 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    39721 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
    39781 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
    39841 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
    39901 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
    39961 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
    40021 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
    40081 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
    40141 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
    40201 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
    40261 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
    40321 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
    40381 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
    40441 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
    40501 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
    40561 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
    40621 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
    40681 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
    40741 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
    40801 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
    40861 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
    40921 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
    40981 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
    41041 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
    41101 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
    41161 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
    41221 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
    41281 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
    41341 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
    41401 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
    41461 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
    41521 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
    41581 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
    41641 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
    41701 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
    41761 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
    41821 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
    41881 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
    41941 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    42001 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    42061 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    42121 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    42181 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    42241 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    42301 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    42361 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    42421 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttctcctgc attcgcatgt
    42481 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    42541 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    42601 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    42661 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    42721 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    42781 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    42841 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    42901 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    42961 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    43021 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    43081 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    43141 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    43201 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    43261 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    43321 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    43381 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    43441 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    43501 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    43561 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    43621 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    43681 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    43741 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    43801 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    43861 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    43921 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    43981 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    44041 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    44101 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    44161 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    44221 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    44281 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    44341 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    44401 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    44461 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    44521 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    44581 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    44641 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaacaaat tctttttgga
    44701 ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac
    44761 aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat
    44821 caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg
    44881 tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa
    44941 tcatcatacc atcgaatttt tatttatgca agccttttca tataaaaaat aaaaaaactg
    45001 gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc gtttctcttt
    45061 ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt taatctgtaa
    45121 cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca gatctcatat
    45181 catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca actagtcgcg
    45241 tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc tcgacttcat
    45301 tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg tatgtataca
    45361 cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct agcggtgtaa
    45421 acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct tttcccattt
    45481 cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc aagcctttga
    45541 aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact ggtagatctg
    45601 ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc gcatcaaagt
    45661 caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca ttcgctatag
    45721 catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg aaaatcaaag
    45781 ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt tttgcttttc
    45841 cttttttttt cattttctct ccctccagac tcattttact gtttttcttt tgctctttcc
    45901 aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc tgcgcttttt
    45961 tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc atctagatcc
    46021 aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctgccag ttcttctttg
    46081 attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa aagttctaac
    46141 aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg aaattgtcgc
    46201 caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg cttgccttct
    46261 tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat agatgatgct
    46321 tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat caataattta
    46381 ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt tgtttttcga
    46441 tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat tttccttctt
    46501 cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga tggatacttt
    46561 catcttgttc taactctctt aactgctggg tcagcatttt ttttgtgatc gtagggattt
    46621 gcttgcttct ccacgcctaa aataagcgat attgctcttc cctttatctg gtgtaagcaa
    46681 agcagttagc attttgacga tcgtgggttc cgcttttcac ttcttatcta cttagtatca
    46741 tgaagtatac tttcgtacta atactaagta gcggaagtgt caactacttt gtttaactac
    46801 atagcaaaag aaacagaagg gtgacaacgg tcatcacgct tctgtttctt ttgaatatgc
    46861 tgtgttgttg tctctatctc cgtgtagact ttcctcaaac ttatcctttg acagtaagtc
    46921 agtaggctga tttgctcttt ctagatgcca gatacatttc tacaaaaaaa tagtgattct
    46981 gttttgaaaa ttcgattttt agttctgcat tttcgtaaac atccaacatt tttttcgcac
    47041 tgtatagacc taacccgcca ttttttttag aggtttttcc tcttttgacc atctccgata
    47101 gcgtcaaatt cgttggattt gccttgttcg ctatactaaa acgtgatccc tctttttctt
    47161 ccagcagatt gatcgtaatc ggactctgat cggctacacg atgatcgaat gcgttagaaa
    47221 taaaaataga taaacagcga ataaaaacaa ccagatcaat cgggattgtt ttcacttcga
    47281 caggaataac tacttggatc gggattttct ccgattctgc tttttctaga aattttaaaa
    47341 agacacccct gacagctggg gatacaatat ttgttagctg ttgtatttgc ggtttttcaa
    47401 aaatagctgc agaatgatct tctaatattt ctaaaaattc caacgtttct tttttatttt
    47461 cctgaagagt aatttttaat gaaaacagta gacttttata atcatgtttg aattcctcta
    47521 gatgatggta atattcttca tttatttgat acagttgaag cgattcctga tgttgtcttt
    47581 cttttacttg gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa ctgatgacta
    47641 tcgacaaaag agatagaagc agataattag atgagcggaa gtgaacactt cccaatacca
    47701 agtaaatcac gccccagctt aataaaatac aaatacctcc aaaatagtgt tcagttggat
    47761 aagtaaaaag caagttgaga aatctcgtct gtttgtctat actttttact aatagataga
    47821 aaataaatgc gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc tttcctaaat
    47881 aatccatcag atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa aaaagattac
    47941 tgatcaaaca gctgataaac aggtttttcg tagtaaaata aaaaagaaac atcaataata
    48001 agccaactag gtaaggaaag ctcactttag ggataggaag taaaagtgtc accaccatca
    48061 aagctataca accaaagctg ttttttacta gcagttgttt cgaaaagaat aaatagatac
    48121 ctaaatctaa aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc atcttattcc
    48181 tccattgttt tgtttcaaaa tgtacacata tttttttaat aggagcctgc atatgaaaaa
    48241 gtattttatc atagaagata accagtttca cagcgaatgg atcatcagag aattggtcaa
    48301 tcatcatatc gagaatatcg tgcagctcac ttctatctct caaatcaatc agttgatcca
    48361 gttgggcact attgataatc acgatgtttt ctttatcgat atcgacttga agcaatcact
    48421 cacaggtatt gatgtcgccg aacaaatacg taaaaaaaat gcacatagta atattgtttt
    48481 tttcaccgct tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc ttccgattgg
    48541 atatatcaga aaagacagga atttatctga acaaattcgt caagtgctag aacagatcaa
    48601 ttggaaagaa cagcagatcg taaatgtcga taaaatcttt attgatcaaa aaggtggaag
    48661 tctcgtcctg tatcctcggg aaattttata tattgaaagt atcaaaggtt tgaaaaacca
    48721 agttatggtc aaaacaacta cagaagataa attagtgaat acttctttaa aacaattgaa
    48781 gacagagtta gaaaagtatg cttacttttt acttttaaaa tcttactgta tcaacttatc
    48841 gcaattgcag aaaattgatt ctgctcatta tgtgttagaa tttttcaacg gtgactcctt
    48901 actcgttggt cgaaagatct ttgaaaagac aaaggaacgt ttccatgact tccaaaaaac
    48961 tgcttcgagt tgatgaataa gagctgagac gtaagcatag cagatttcag ggagtgggcc
    49021 ttgaatcggc tccactccct gaaatcgttg atatgatttt acgcttcctc ttctgtagga
    49081 gcatctgtta acgaccatgt gaatgttgaa acgtagttgt ctacagcata attggataca
    49141 ggaacaaata attccactga ttgtctccct tgttccacat tctctcctaa tgaataaatc
    49201 catgtgccaa tcccttcatt tgctcctgca gttactagat ctactgcctg atctgctggg
    49261 atttcgattc cttcagcgcc agttccagaa acggtagatg gcattccact gctattagtt
    49321 gcgctatttc gaatcacggc gtttttgaat ttcacctttg aaccagttag tttattcgta
    49381 tcagatgttg taccaataaa ttctgttcgt ttgactgata atttccaacc ggcataattt
    49441 ccgcttttat ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc agtactgtcc
    49501 gttccgcttt gtaatgcagc atatactgtt tttgctgtac ctaacttgat tgttccaaaa
    49561 tcaagatctg gagcttggtc aatcgtcagt aatccggatg ttccctgatt tggttcgtca
    49621 ggtgtaaaag gagtatcagg atcatttgga tcaacaggag gaactggaac aacgtcttca
    49681 ggatttgcag catctggttt ggtcacagaa atcgttcctt ctgtagtggc tgagctctta
    49741 ttctcagcaa aaacactttg tgtacataat aatagacttc cagctagaac ggtacttaat
    49801 gcaattgttt ttttcatttc tttttccttc tttcttttaa aataaataat ccagcaaata
    49861 agccaataag aatgactcca acaagtatca gccatcgcca gtcaaatgtt tctggctgat
    49921 tttttaaatc aatatccttt tcattcaatt ggtccgctgc atcatcagtt atttcaaaat
    49981 cggtatggag attccagcgt ttttctttcc aagcaccctt cacaaccgct tgataatgtc
    50041 ccggagctaa tttttttcct tctaatggga caagtaaatc aaaatttgta ttgggtgcta
    50101 catctatttt ttcttttttt attgaaaggt aaggctcttt tgtttctttt ttgtagattt
    50161 caaccgatgt agtcaagccg aacatataag aagcatttgg attttgaaga ttcgccttaa
    50221 tcacatttct tccatcgctt tgatccgcat aagctttttt taatttcaaa tctggggaag
    50281 actctttttc gctatttcaa atcaagattg ctttcacttg agaaaactga ttgacgacac
    50341 caactgtact tttgttagaa acgttctctt ttttttcttg aaacgtgatc cctcctgcga
    50401 ccacaccagc cagctctttt tctggcgctt tcagtgaaag tgtctcagtg accgcacttt
    50461 gaggaggaac agttatttct cccactgttt ttgctaagtt tttcatatca taacttaatg
    50521 tttcatcaaa tttacttttt gattctccat attcgatcac accgtttgaa ttcgtcgtag
    50581 cggagtggat caatgtctca actgttactg atttatctgt tcggttaaag accgtgtagg
    50641 ggatttcctg tgttttccca ggctccatcc ttacatttat atatgttttg ttcttatcaa
    50701 tctgattagc cggtaattca aaatctaccg caaaattgaa ttcggaggca tggacaggtg
    50761 tctgccaaag gatgaatcca aggagaagga tcataagtaa agagcagttg tttcgatgac
    50821 ttttattcca tattcgattc atcaatcatc ctccttgatc tttattctgt cggcgcatct
    50881 gctaatgtcc atgttatctc tgctgagtaa ttaccagcta tctgattttc tttggttaag
    50941 acaagtttaa acgcccgacc gttatttaaa tattctgaat aatctgcttg cacattcgtt
    51001 gaattttgtc ctctgacaat ttccagttct tcttcattca ctaattgata ctcctctcct
    51061 ttcttgaaat ataggacatc ctgcatagaa aatcctgcat catttgtgaa aggctgagat
    51121 aattttatcg ttaagatcca cggatttttc gtttctcgct gatcataaat ccataatttt
    51181 tcctgtatat ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt gatctgacca
    51241 aaatcgagtt tggaaggggc aagaaatgag agaccacctt ctactacgat cttcgtagca
    51301 ggtgcaaaag gcggggcatc atgataaaca actggcgtat cacttggatt gtgattccca
    51361 atttcatcgt tggtacttgg cttgttctgc acacccttag cagtagcttc tcctgcttgg
    51421 tcgtttaata caatttctaa tacatctcct gcaaaaagtt gattttctgg gatagaaaga
    51481 acaaattccc ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt ttttttcaat
    51541 gaaaccaagg ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc attgatcaat
    51601 gtggtactta aattagcagg ttcaggagga atcacatcga ctgtcgtttt ggcagagtcc
    51661 agttctgtca cttcttggcc acctcttact tttttagcag caacaacttg cacttgatcc
    51721 tccgttttta atctctcatc attgggtact tgaacttcga acaagccgcc ttgttcgcct
    51781 tctccccatg ggttatatcc tgccccttca ttttcatctg tttgtccttg cgtgagagca
    51841 gtaatttcta cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt cacttctact
    51901 tcgcctgtcc aagcatctct tggctttgcc tcgacacctt cgggaacaac gacatgccca
    51961 tagatttttt tatctgcatt cgttggcact cttaaggatt caatcatcgg gagctgatta
    52021 ttggcactga ttcgattata atttttcatt ccggcacctg tctgagcaaa ttcatttgtt
    52081 aaacctgcac tagggcttga tacgaaattg cctaagtcgg tcccattgat cgataagtct
    52141 gtcatatcag aaaaataatc cgggtctttt tcaaagttat cgaatgttgc gcctgttttt
    52201 cgccataaag cgatttctga atgggcgatc tttaaagaag aatctccatt tccttggaat
    52261 acccagccgc ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa atcaaaatag
    52321 gtaggagatt ctaagtccac attcaacaca tccccactga tgatgccggt cttatcttcc
    52381 cctgttttcc cgaccatgac taaataagtt ttttcacctg ccgtaaaatt taaatcaata
    52441 tctccagttg tatctacagt tgctccggaa tctgcgcgta tatcgatatt tgatttttct
    52501 ccagtgactg taaaatcagc tgaacctgga ctttctagaa aaccagaagc gtcataaaat
    52561 tggatcgctt gatttccttg attgagtctg ttcacgtaag atttgccatc acctgtattt
    52621 cttaccctca atgagcctcc attggttact gtcagctttt gcgtaccatt ctcaaaacga
    52681 actgcgctgg aacgcccact ctgcttatcg atgtcgataa ctgatccggc tccttctacg
    52741 atcaaagaaa ggccttttcc tacaaaacgc atcccattgt tcccgggttg atcgtcatct
    52801 ccagccattt tgaccgagat ttgtccacca cttgatacat ggatttgact agaatctcct
    52861 tgcaagcgaa atccgcccgt agttgctgta ttgatggtga ttttcccacc attcgataca
    52921 gtgactttcg atcctgaacc tctcatttgg atgattccgc gattgttgtc attggtaata
    52981 ttgatctttg tatttaccga aaattcgctt cccgaaccac tgacttcaaa catgacatta
    53041 ccaggtagcg tatcgactgc ctctccttca tcgctaacgc catctgcccg atcataatca
    53101 agagagacct ttgaaccgct taagatatgg attcctggcg ctgtttttcc actaccagtc
    53161 tcatctcctg aatctagaaa accgtagaat ttccctagat acggtccata aagttgacta
    53221 ccattgatca cattcaattg atagcttcta caaaaatagg ctttacccgg agcggcattc
    53281 agtccttcct tcactggttg gacatccaac acaccggaaa caccatctaa tgtgacagtt
    53341 gcacgaggag cgtaaatcag tcctaatttg gacccatcat acaaatcaag tgtcccggta
    53401 aattttattt ctcccgtaaa atgatatctg cttccccaaa aatcggtcat ataagcaatg
    53461 ataatggcat ttccatcgat atttggtgtc aatccacctg gatgtgcagc tgttccttta
    53521 aaattctgga actcgatcga ctgaggtatc tcattgatat aaatatggtg tgtgccgaaa
    53581 ttgtgagcga atccaccaaa atcaataacg atatctcgtt tgatctctaa ttccctgtct
    53641 aaagtcaaag gctgaatcag tcggatctta gttactgatt cgtctgcgag aagctgcgcc
    53701 agttcttctg ttgtggcagc ttcgctgtac ccatccgctg aaacagctct tgccagtgcc
    53761 gcttcactgc tatcagacgt ttgttcagct acttctgagg tctcacttgt cttattttct
    53821 tcttgccttt tattagtaga agcctcactt tgagattctg tagtttcaga agtaatcgat
    53881 tcttctgaaa aagtagtctt gtcaaaactt tgattctctg attgcgttga ttcgtttgtt
    53941 ttctctacat cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa caaaatcgac
    54001 gcaatcagct tttttacaca ttgattcatt ctccaccatc ctctctgacg ttttgacaat
    54061 tttattttat cgttttaatc tatttccaat tgtactttca tatttaaaat atgtattttt
    54121 atggaaaaat ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa ggctgtgaca
    54181 aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc
    54241 acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg
    54301 taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga
    54361 cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt
    54421 taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa
    54481 atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga
    54541 gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc
    54601 tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc
    54661 tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga
    54721 gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt
    54781 gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc
    54841 tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg
    54901 ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact
    54961 atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt
    55021 atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa
    55081 ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac
    55141 tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc
    55201 tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa aacatccata
    55261 ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt
    55321 gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa
    55381 tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa
    55441 atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg
    55501 cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt
    55561 ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt
    55621 atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc
    55681 tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa tatctagggt
    55741 cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtagtaatta
    55801 aactactgtt gatttagttc taataaaaag atttagatga acagttttat tgatttaaat
    55861 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
    55921 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
    55981 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
    56041 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
    56101 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
    56161 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
    56221 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
    56281 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
    56341 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
    56401 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
    56461 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
    56521 caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt tctttggttg
    56581 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
    56641 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
    56701 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
    56761 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
    56821 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
    56881 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
    56941 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
    57001 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
    57061 tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg ttcgattttc
    57121 cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa atgaatatat
    57181 taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct attttatata
    57241 aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat gtgtcagttc
    57301 tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct gatcgaatag
    57361 agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag tggttagaat
    57421 gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca gagctatgat
    57481 taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata atattacctt
    57541 tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca ccgtgaactg
    57601 aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc cctagtgcac
    57661 aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt taaagcatga
    57721 tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata actaacgtac
    57781 cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt ttatccaatg
    57841 acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact ctcgcacagg
    57901 cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc ttcttgttta
    57961 tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca tttaaattcg
    58021 aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc aacccaaaat
    58081 cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc ccagttaatt
    58141 gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca ttcaatccta
    58201 atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc acaagatccc
    58261 cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg
    58321 caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc aataactgac
    58381 ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga
    58441 aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag attacacttg
    58501 tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca aagagcgaat
    58561 aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat atgcataaca
    58621 tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat
    58681 gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac ggaaatgata
    58741 actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt tctttcgaca
    58801 aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata tttctttcca
    58861 aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga
    58921 tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt
    58981 tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat
    59041 gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca
    59101 accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc
    59161 ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc
    59221 cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa cgattggaat
    59281 aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac
    59341 cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag
    59401 ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa
    59461 gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa
    59521 gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc tggaacaaat
    59581 aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga
    59641 atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagt
    59701 cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa
    59761 ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg tctgtatttg
    59821 aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag
    59881 tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat gaaccgttca taggcttctt
    59941 tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa
    60001 tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat
    60061 ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat
    60121 ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga
    60181 cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca
    60241 atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct
    60301 gtttatttat actaaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta
    60361 aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa
    60421 aatcataccg tttttcatca aaaaaacgta tctatcaagt gtataggtca tgtagaataa
    60481 atctatatac tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa aatacgaact
    60541 taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg tgtttccgat
    60601 aaaacgattt ctagttggga aaattctcgt acaatgccag accttgaaat gctgtcactt
    60661 ttacatcata ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca
    60721 atagaaccaa cattgccaga tgaacctctt gttaacaaga aatcttattc atcaaaacaa
    60781 cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt
    60841 tttcttatca attatcaata caaaaatgaa acgatcgatc gattcaatcc ttttttgaaa
    60901 ccacaaatcg gctataccac actccctgta ggatacgtgg acaattccat cgaacgttgg
    60961 gtatgtaatg atatctttgg ccatggtaca tggctgtctt ttcacgctgg agaggtagaa
    61021 aaagagaaaa accatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg
    61081 ctcacttggg aagccattcc ctcagattac caagaaatcc tcagccaaga ctatgacgac
    61141 gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga acgtagagat
    61201 agaaaaaatc aatgcacatg atcttatcca aaaaacaggg gtacaaatta attgaaaaag
    61261 ccttgagatg aaagttccta aataaaaaag ttcagaatga taggtatttc tggcttatag
    61321 tataatattt acgccacagc ctcccaatga ttctttttgg aatcataatc aaggatggac
    61381 attcacaaaa atattttgct cattcgatcc gaatagatta acgaccaatc tagtaagtaa
    61441 tatagattgg tacgtagtca agctgcacat aatttagatc aattttagtt cttttttata
    61501 tttctatgat ctgctcattt acttttatat gatggcttac agtaacataa ataaatatga
    61561 taatcgctgg tagtttatcc aagagtactc aataaaatac aaaatggatt gttcaagcgc
    61621 ctatcataat gataaaatga aacaaataaa cgatacaagg aggaagagac tttgagtaat
    61681 ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt aatctatggg atttcggtag
    61741 catgggcatt tttttataga ggaatcacac caacctggcc gttttatctc ttgttagtgg
    61801 gaatattagt aggaaatttt gcccctcttc actatcgtac gaactttttt ccaactgatt
    61861 ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa cattattgaa atgacaatta
    61921 cctgtataat cattttctta gttatattcc taaacgtctt ctaataagaa tctcttaaaa
    61981 ttagcttgtt acagttagtt acctagttta tataacacag ttatcttatc ttccttatca
    62041 acatcttttg ccaactcttt gtcttaaatt cgaaacatat aagcagagct acgaacacca
    62101 agactgtttc ggctctgttt tttgcatcaa attcactctt atatagctga taatctcggc
    62161 tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa ccttgatgtt
    62221 atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat agatttttaa
    62281 attacacact agtttaaact aaaacaaaca aggaaacact taccatttaa tcataaaatt
    62341 agctcatatg aatatattag aatgtaaaat agactggtca aaatctggtc aaaaaagagg
    62401 actaatacag gtagt
//
LOCUS       DADVXN010000004        60336 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.004, whole genome shotgun sequence.
ACCESSION   DADVXN010000004 DADVXN010000000
VERSION     DADVXN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.004"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1421)
                     /locus_tag="KB942_000201"
     CDS             complement(<1..1421)
                     /locus_tag="KB942_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6850656.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="KB942_000202"
     CDS             1673..2854
                     /locus_tag="KB942_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6850657.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="KB942_000203"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="KB942_000203"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBB6850658.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="KB942_000204"
     CDS             complement(4993..7704)
                     /locus_tag="KB942_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850659.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="KB942_000205"
     CDS             complement(7714..8415)
                     /locus_tag="KB942_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850660.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="KB942_000206"
     CDS             8601..9200
                     /locus_tag="KB942_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6850661.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="KB942_000207"
     CDS             9281..10294
                     /locus_tag="KB942_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6850662.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="KB942_000208"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="KB942_000208"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBB6850663.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="KB942_000209"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="KB942_000209"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBB6850664.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="KB942_000210"
     CDS             11742..13115
                     /locus_tag="KB942_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6850665.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="KB942_000211"
     CDS             13472..15616
                     /locus_tag="KB942_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6850666.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="KB942_000212"
     CDS             complement(15668..16708)
                     /locus_tag="KB942_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6850667.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="KB942_000213"
     CDS             complement(16985..18787)
                     /locus_tag="KB942_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850668.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="KB942_000214"
     CDS             complement(18801..19553)
                     /locus_tag="KB942_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850669.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="KB942_000215"
     CDS             complement(19562..19924)
                     /locus_tag="KB942_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6850670.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="KB942_000216"
     CDS             complement(20100..20972)
                     /locus_tag="KB942_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6850671.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="KB942_000217"
     CDS             complement(21023..21511)
                     /locus_tag="KB942_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6850672.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="KB942_000218"
     CDS             complement(21649..22440)
                     /locus_tag="KB942_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBB6850673.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="KB942_000219"
     CDS             complement(22495..23103)
                     /locus_tag="KB942_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBB6850674.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="KB942_000220"
     CDS             complement(23096..23413)
                     /locus_tag="KB942_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6850675.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="KB942_000221"
     CDS             complement(23569..24297)
                     /locus_tag="KB942_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBB6850676.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="KB942_000222"
     CDS             24456..25337
                     /locus_tag="KB942_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6850677.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="KB942_000223"
     CDS             complement(25574..26617)
                     /locus_tag="KB942_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6850678.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="KB942_000224"
     CDS             complement(26620..27288)
                     /locus_tag="KB942_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6850679.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="KB942_000225"
     CDS             27430..29766
                     /locus_tag="KB942_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6850680.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="KB942_000226"
     CDS             complement(29881..30192)
                     /locus_tag="KB942_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850681.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="KB942_000227"
     CDS             30373..31848
                     /locus_tag="KB942_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBB6850682.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="KB942_000228"
     CDS             31895..32479
                     /locus_tag="KB942_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBB6850683.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="KB942_000229"
     CDS             32481..32864
                     /locus_tag="KB942_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBB6850684.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="KB942_000230"
     CDS             complement(33181..33465)
                     /locus_tag="KB942_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBB6850685.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="KB942_000231"
     CDS             complement(33817..34527)
                     /locus_tag="KB942_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBB6850686.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="KB942_000232"
     CDS             complement(34524..35996)
                     /locus_tag="KB942_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6850687.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="KB942_000233"
     CDS             36134..36607
                     /locus_tag="KB942_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6850688.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="KB942_000234"
     CDS             complement(36760..37311)
                     /locus_tag="KB942_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6850689.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="KB942_000235"
     CDS             37416..37946
                     /locus_tag="KB942_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBB6850690.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="KB942_000236"
     CDS             complement(38429..38872)
                     /locus_tag="KB942_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6850691.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="KB942_000237"
     CDS             complement(38888..40048)
                     /locus_tag="KB942_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6850692.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="KB942_000238"
     CDS             40555..42255
                     /locus_tag="KB942_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850693.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="KB942_000239"
     CDS             complement(42357..43190)
                     /locus_tag="KB942_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBB6850694.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="KB942_000240"
     CDS             complement(43494..45659)
                     /locus_tag="KB942_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6850695.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="KB942_000241"
     CDS             45919..48213
                     /locus_tag="KB942_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6850696.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="KB942_000242"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="KB942_000242"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6850697.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="KB942_000243"
     CDS             48864..49871
                     /locus_tag="KB942_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6850698.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="KB942_000244"
     CDS             49893..50912
                     /locus_tag="KB942_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6850699.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="KB942_000245"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="KB942_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6850700.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /locus_tag="KB942_000246"
     CDS             complement(52669..54090)
                     /locus_tag="KB942_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6850701.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="KB942_000247"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="KB942_000247"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6850702.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /locus_tag="KB942_000248"
     CDS             complement(56501..57757)
                     /locus_tag="KB942_000248"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBB6850703.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="KB942_000249"
     CDS             58337..59638
                     /locus_tag="KB942_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6850704.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="KB942_000250"
     CDS             complement(59707..60306)
                     /locus_tag="KB942_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6850705.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18211 a        12349 c        10817 g        18959 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa actttt
//
LOCUS       DADVXN010000005        55411 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.005, whole genome shotgun sequence.
ACCESSION   DADVXN010000005 DADVXN010000000
VERSION     DADVXN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 55411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.005"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            137..994
                     /locus_tag="KB942_000251"
     CDS             137..994
                     /locus_tag="KB942_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBB6850706.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="KB942_000252"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="KB942_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBB6850707.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="KB942_000253"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="KB942_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBB6850708.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="KB942_000254"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="KB942_000254"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6850709.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="KB942_000255"
     CDS             3750..4508
                     /locus_tag="KB942_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6850710.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="KB942_000256"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="KB942_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBB6850711.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="KB942_000257"
     CDS             5436..5735
                     /locus_tag="KB942_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850712.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5761..6468
                     /locus_tag="KB942_000258"
     CDS             5761..6468
                     /locus_tag="KB942_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6850713.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="KB942_000259"
     CDS             6465..7232
                     /locus_tag="KB942_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6850714.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="KB942_000260"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="KB942_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBB6850715.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="KB942_000261"
     CDS             9197..9517
                     /locus_tag="KB942_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBB6850716.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="KB942_000262"
     CDS             9517..9867
                     /locus_tag="KB942_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBB6850717.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /locus_tag="KB942_000263"
     CDS             10034..10972
                     /locus_tag="KB942_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBB6850718.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /locus_tag="KB942_000264"
     CDS             10987..11817
                     /locus_tag="KB942_000264"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBB6850719.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="KB942_000265"
     CDS             11861..12886
                     /locus_tag="KB942_000265"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6850720.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="KB942_000266"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="KB942_000266"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6850721.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="KB942_000267"
     CDS             13964..14404
                     /locus_tag="KB942_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBB6850722.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="KB942_000268"
     CDS             14407..15009
                     /locus_tag="KB942_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6850723.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="KB942_000269"
     CDS             complement(15077..16183)
                     /locus_tag="KB942_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6850724.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="KB942_000270"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="KB942_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBB6850725.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17697..20042)
                     /locus_tag="KB942_000271"
     CDS             complement(17697..20042)
                     /locus_tag="KB942_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6850726.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20321..21223
                     /locus_tag="KB942_000272"
     CDS             20321..21223
                     /locus_tag="KB942_000272"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBB6850727.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21436..23211
                     /locus_tag="KB942_000273"
     CDS             21436..23211
                     /locus_tag="KB942_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBB6850728.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23468..24685
                     /locus_tag="KB942_000274"
     CDS             23468..24685
                     /locus_tag="KB942_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6850729.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25132..26718)
                     /locus_tag="KB942_000275"
     CDS             complement(25132..26718)
                     /locus_tag="KB942_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBB6850730.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26969..27193
                     /locus_tag="KB942_000276"
     CDS             26969..27193
                     /locus_tag="KB942_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBB6850731.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27335..29086
                     /locus_tag="KB942_000277"
     CDS             27335..29086
                     /locus_tag="KB942_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6850732.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            29086..30870
                     /locus_tag="KB942_000278"
     CDS             29086..30870
                     /locus_tag="KB942_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850733.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31328..32179
                     /locus_tag="KB942_000279"
     CDS             31328..32179
                     /locus_tag="KB942_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6850734.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32276..34162
                     /locus_tag="KB942_000280"
     CDS             32276..34162
                     /locus_tag="KB942_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6850735.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34203..35105)
                     /locus_tag="KB942_000281"
     CDS             complement(34203..35105)
                     /locus_tag="KB942_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6850736.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35335..36738
                     /locus_tag="KB942_000282"
     CDS             35335..36738
                     /locus_tag="KB942_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6850737.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36735..38171
                     /locus_tag="KB942_000283"
     CDS             36735..38171
                     /locus_tag="KB942_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6850738.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38316..39242
                     /gene="trxB"
                     /locus_tag="KB942_000284"
     CDS             38316..39242
                     /gene="trxB"
                     /locus_tag="KB942_000284"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBB6850739.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39455..41182
                     /locus_tag="KB942_000285"
     CDS             39455..41182
                     /locus_tag="KB942_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBB6850740.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41198..41548
                     /locus_tag="KB942_000286"
     CDS             41198..41548
                     /locus_tag="KB942_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6850741.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41632..42492)
                     /locus_tag="KB942_000287"
     CDS             complement(41632..42492)
                     /locus_tag="KB942_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBB6850742.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42584..43012
                     /locus_tag="KB942_000288"
     CDS             42584..43012
                     /locus_tag="KB942_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6850743.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43174..43350
                     /locus_tag="KB942_000289"
     CDS             43174..43350
                     /locus_tag="KB942_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBB6850744.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43377..43823
                     /locus_tag="KB942_000290"
     CDS             43377..43823
                     /locus_tag="KB942_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBB6850745.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43979..45358)
                     /gene="brnQ"
                     /locus_tag="KB942_000291"
     CDS             complement(43979..45358)
                     /gene="brnQ"
                     /locus_tag="KB942_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6850746.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46064..47035
                     /locus_tag="KB942_000292"
     CDS             46064..47035
                     /locus_tag="KB942_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBB6850747.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47059..49251
                     /locus_tag="KB942_000293"
     CDS             47059..49251
                     /locus_tag="KB942_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBB6850748.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49268..49744
                     /gene="ybeY"
                     /locus_tag="KB942_000294"
     CDS             49268..49744
                     /gene="ybeY"
                     /locus_tag="KB942_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBB6850749.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49728..50123
                     /locus_tag="KB942_000295"
     CDS             49728..50123
                     /locus_tag="KB942_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBB6850750.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50138..51037
                     /gene="era"
                     /locus_tag="KB942_000296"
     CDS             50138..51037
                     /gene="era"
                     /locus_tag="KB942_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBB6850751.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51180..51992
                     /gene="recO"
                     /locus_tag="KB942_000297"
     CDS             51180..51992
                     /gene="recO"
                     /locus_tag="KB942_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBB6850752.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52376..53293
                     /gene="glyQ"
                     /locus_tag="KB942_000298"
     CDS             52376..53293
                     /gene="glyQ"
                     /locus_tag="KB942_000298"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBB6850753.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53295..55370
                     /locus_tag="KB942_000299"
     CDS             53295..55370
                     /locus_tag="KB942_000299"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBB6850754.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17957 a         9933 c        11785 g        15736 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgtattct attaaatcaa
    17701 tcatctttct catctgattc tttggttgct tgattcgctc ttgcataagc tgaagcggtg
    17761 ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa ggcgcttttc
    17821 ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt
    17881 ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc
    17941 acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa
    18001 gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt
    18061 ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt
    18121 agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct
    18181 agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc
    18241 acactgcgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag
    18301 ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag
    18361 ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cattggtctg ttgaagcgtc
    18421 gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag
    18481 ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca
    18541 tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt
    18601 tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct
    18661 tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg
    18721 attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg
    18781 aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg
    18841 ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct
    18901 aacatgtatc cataattttc tacgccattt tgcaatgcag catagacatc tttatcgatt
    18961 gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg
    19021 tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg
    19081 attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg
    19141 atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct
    19201 ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg
    19261 gcagaaagat cttcttttag cgcacctgtg ttcgtgtatg gactatacgt gatcgggctt
    19321 tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc atctacacca
    19381 aagattcctc ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca
    19441 aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact
    19501 tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc
    19561 tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct
    19621 tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat
    19681 tcatcctctg tgctgataat tgcagttttc aataaaggtg aaattttatc agatgaaata
    19741 gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc
    19801 tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt
    19861 gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac
    19921 atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta
    19981 ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc
    20041 aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa
    20101 aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa acgtgagaaa
    20161 atgatctttt ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg
    20221 gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg
    20281 aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt
    20341 cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc
    20401 ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga
    20461 gatgaatatt gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa atatcgtcgt
    20521 tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc
    20581 cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac
    20641 acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta gaattattga
    20701 aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg agacaatggc
    20761 aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt
    20821 aaaattgaat gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta
    20881 taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga
    20941 tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg
    21001 gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga tcgtccatga
    21061 tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtcggca aagataaaaa
    21121 gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcacaaa tattcgatcc
    21181 agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc tatctgacag
    21241 cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtgc
    21301 tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacag
    21361 ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat
    21421 tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact aaaaaagaaa
    21481 catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat
    21541 agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct gctgattcgc
    21601 tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc
    21661 aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct ttcttatgca
    21721 gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg
    21781 atcgtgaaga acaagtatct tcttttaagt ttctattttt attttttgtt ttctccgatt
    21841 agttttacac atgaatattc cactcgaacg tatcgtaatg ccctcgtgat tccggctgtt
    21901 gagatcatta tcgcttgttt actagctatt tattaccgta aatatagttc cagcaaaaaa
    21961 atgctgccgt ggtttttagg gctagctgtt tcttttcctt tcttttggtt cattcgcgaa
    22021 gattccatct ggcttcttcc ttttactgta gtcgcgttag gcatcagttt cgtccagatc
    22081 ttgctaggac aagacttttc tttcaaagaa gcaaagaagt taaaagaacg accatttctc
    22141 ctttcgaaac aagaatggct gaaactttca ttgtttttac tccctctagt cggtttctcc
    22201 atcgtctatc agtggatcga atataaaaac gagacaacat atagtattgc cgccatcaat
    22261 gaccgttcga gtggcgaatt tggacgtttg atgaaacagc tgatccgaat agatgacgaa
    22321 acgaacttaa ataaagaaaa cagtgagata tgggtctcgc atgctgcatt aaataaagca
    22381 ttagacgcat ctcctacatt tcatgctgtt tctactcgta ttgacgaatt gtatacgact
    22441 catgcgtgga cccaaggtgg aaaagtaaaa gaattaccag gagatatcat tttttgggca
    22501 ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat
    22561 ttttggaaaa aagtcaatca agaattagaa gaagcctatc aaaagggaac tatcaaaaag
    22621 aaaaaagaat tctatctgat gggatcaggg gatggaaaga cgatgaatga tctgccaatc
    22681 gtatttgctt ttctgaaatc aggatatgcc tatgcatggc gatacaaaga tttccatcaa
    22741 ggggggaatt atagtttcgg tacgcaagaa caagtcgaac aggcccaaga attattgaat
    22801 cttccactaa tggataattg gatcgatagc agtgatccga gaacacctga tttccagttg
    22861 acaaaaacag caaaagtcgc gaatgtcatg ataaaaattt accaagttct ggccattccc
    22921 gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgc atttgcaaaa
    22981 gctgatcgtg cctattatag ttcctctttg attgtagtca ccggattaat actgacacaa
    23041 ttggtttttc ttttcggcgt ctcgtggttt tgttcttatg cgccagaaaa aaaggattat
    23101 tttttaagtg tctacactgg cgcaggcgtg ccattaacgc aattcatcgt agtaacaagc
    23161 atgctagttt tcagtaaatt gaatctgaaa aaagctttat cttccaaata aaaacatact
    23221 aagattagag gagaacatca ttgacgaatg atgttctcct ctattttcta tttttagatt
    23281 atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg actatgagtc cctatcaaaa
    23341 accgaacttc tttactgtca ttttagaatc aggtttacgt atgttggggc catgagcccg
    23401 tgtataggaa attggaatcg aaatgaagca gtaaagttct ttcaaaactt aaaaaggagg
    23461 aaaacaaatg ccttacattc ttgcattaga tgtttcaatg ggacacagct acagtgtcct
    23521 atacaaagac gacacctgct tatttgaaga cgagatcgaa cacactcaaa gaggatttca
    23581 tgcattactc gaagagatcc agcacttgcc tgaacgtcca ttgattgttt ttgaagcaac
    23641 ggggatttat tccagaccag tcgaaaagtt ctttcaagat catcactttt cttactgttt
    23701 gttaaacccg ttagaagcga aaaaacagat ggaagaaaac accttatgca gttggaaaac
    23761 agataaatcg gatgcccatc ggctcgcaca gactcatctt aaaaatcaac gtcagccaaa
    23821 agaagtacaa agcaattttt acttagagat gcgcgattta gcccgttttt atcaagaaat
    23881 tgagaagaaa atcactcgtc tgcgcatgga tttacacaac tgccttcagt tgacttttcc
    23941 agagcttgaa cagttctttt taaacaggct gaccccttac gcattgatat tgatccgtct
    24001 ttttccccat ccagattttg tattggcatc tactcggaca aaaataaaaa ataaactgat
    24061 caacgaaaca cggaaaaaaa tctccgcgaa tcgtgccgag caaaaggcag atcaaatcat
    24121 ccactatgca cagtgtgcct atccagctgt tgaaaaagac agtatccact gtcaaaaaac
    24181 gatctattac gctgaacttc tccaagattt actcgaacaa aaagaagcac tggcgacaca
    24241 aatgatcaaa aaagccgaag gctctccttg ctttttgttg taccaaacgt tccctgggat
    24301 tggcgcactc acagccgctt tattgctcgg tgaattagga gatatcacac gatttaaaac
    24361 ccataaacaa ttgaatgcct ttgtcggaat cgacatccga cggtatcatt caggtaagta
    24421 tactggccaa gaccgcatca ataagcgtgg taatcccaaa gcacggaaaa ttattttctt
    24481 caccattcga aatatgattc gccagcaacg cgcagctcct aatcatattg tcgattatta
    24541 ctacaaatta aaaaagcaac ctatccctaa gaaagataag gttgccacag ttgcttgtat
    24601 gaacaagctt ctgaaatgta tgcacgctat ggttagggca catacagagt acgactatgc
    24661 gtacgcggtc tcagtggacc attaactaaa ccacttacag tataccactt ctttgcccag
    24721 atcgaaaatt ttttacgaac ggggctttat ttggtgtgcc ttgattttgt cgctttcgtt
    24781 tatgagctat ttttcttaca ccttttactt gactaatcgt aggaaaggga actgtgacaa
    24841 aagtcttgtc gcagttcctt tttccgaata aacggtgctc aaaagcttgc atctgcggta
    24901 ataagtccaa ccaagttaaa aacataaaaa gctgttttta cttggctgtt cttattactt
    24961 gatgcaaggc agctttttca caacctctga tacacggtgt tccagaagta acttcttcgg
    25021 aaataagccg aaatttcaca aaaatttgaa aagcaatttt cttaaattcc ttcttatttc
    25081 ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa agaatcagtt tctactcgat
    25141 ttcttctatc gtttcctgca aacgctgttc atattttttg tttcctttat ataaatcacc
    25201 aatcgtccat gccaacagga gaaggacaag aacaatgatt acataagcga tttgatcagc
    25261 caatgctagt tgagctgagg tggcttgctc gccgaagaaa ctttcgattt gacctggtaa
    25321 tcctcgtaaa ttcaagtaag tcagactaat cacagagaac cagccaagta gttttacaac
    25381 aaatgaattt ttgaatcggc tccccatctc cgcttggcta ttggtcatca tcagtaaagg
    25441 aatcatcgag aatggcaatg caaaagccag aaaaacttgg gattcattca tcaagttatt
    25501 caaagcggta tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac
    25561 aggaatcaca gaaagcaatc gtgtgactag tcttcttagc caaattggga tacgcatatg
    25621 gatgaagcct tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc
    25681 agaagcaaga agtgctacag caaataaagt agacaagata ccagattttg ctacagcaat
    25741 caagatacca ttgctcatag ctgatggatc agataaagct tcatacagac caaaaaagga
    25801 aggatcttgg acagaaccgg ttttgaatac agccacaccc atgatcaata gcagtgcatt
    25861 gactagaaaa gccattgaca actggatatt agaatcccaa gtagaaaagc gtaccgcttg
    25921 tgctacatct tcttcgtctt ggtgatcgat cttccgagtt tgagaaactg ctgaatgaag
    25981 atataagtta tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt
    26041 ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg ttagggataa atccggcaaa
    26101 caaactgtgc caatcagggt tagacaagcc aacttgatag acgaaaacga ataaaatgac
    26161 gaaaatcaag cagacaacca aagcttcgat tttacgaaac ccgacttttg tcagcaataa
    26221 aaggacgaaa acatctaaca cagtgataaa tacggcaatt accaatggaa tatcaaaaag
    26281 caggtagagg gcgattgctg caccaatgac ttctgcgatg tctgttgcca taatggctaa
    26341 ttcagtcaag atccataaaa tgacacccaa tgtcttactt gtccgtgctc ggattgcttg
    26401 ggccaaatcc atttgggtca cgattcctaa ttttgctgcc atatattgta aaagcatagc
    26461 aatcagacta gacatcaaaa taactgacat caataaatat tggaaatttt gtccaccagt
    26521 gatcgaagtg gaccagttac ctggatccat ataaccaact gctactaatg cacctggtcc
    26581 agagtaagca agaagtgttt taaaaaaact ttttcccttg ggtacatcca ctgttccatt
    26641 tacttcagct aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt
    26701 gttggattga ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca
    26761 cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc taattttttt tcgtggcgtg
    26821 tcttgctggt taatgaaaga aattttgttc ttctctaaaa aaaccactat tttttgcatg
    26881 aaactaaaga atacgccttt cttttttcaa taaactttag taaaataaat cattgagagc
    26941 aattcggaaa tagaaaggag agatttatat ggtatcaaca ggtattgtcg tacttattgc
    27001 tattattgct ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa
    27061 agactattta gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc
    27121 aatggggcaa aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca
    27181 agggaaaaaa taggttctga cccaaagact ttttgcgggc tgattttttc agtccgtttt
    27241 ttatttcgta atttgttaga ctattcagac tattcagcaa aaaagacaag cacagacaat
    27301 cctatgctct tgataaagac aaaggaggag taaaatgtcg atttttagaa aattaggttg
    27361 gttttttcga caggaaaaga aacattattt aattgggatt ttctccctat tcgcagtggc
    27421 cttggtacag ttagctcctc caaaagtgat tgggatcatc attgatgaga tcgccgataa
    27481 aaatttatca ataaagatca tttcctggtg gatcttatta ttgatccttg cagctctctt
    27541 acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga
    27601 gaaagaactt cggcagcagc tattcaaaca ttttacagaa atggacaatc tcttttacca
    27661 aaaatatcgg actggagatt tgatggctca tgcaacgaat gatttgaatg caatccaaaa
    27721 tgtagcagga gctggtatct tgacctttgc agattcagtg atcacgggcg gaacaacgat
    27781 cattgcgatg attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc
    27841 tttactagcc gttacttcac gagtgctggg gtcaaaactc cacgatgctt ttcgtgattc
    27901 gcaagaagct ttttctagta tcaatgataa gacacaagaa agtattactg ggatcaaagt
    27961 cattaaaaca tttggacaag aaaaagaaga tctcgcggat tttgaagaaa agattgaaga
    28021 tgcaattatt aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac
    28081 cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga
    28141 aggcacgatc tcaatcgggc agatggtctc atttattagc tatatcggta tgcttgtttg
    28201 gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga
    28261 tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg gaacaactgg gtgcagtgca
    28321 gactccggca aaaggcgagc tgaatgtagc tatttcctct tttaactatc cgaaagaaga
    28381 atcaccagca ttagaacaaa tcaaatttgc gatccaagaa ggagaaacat taggtattgt
    28441 tgggaaaact ggtgcgggaa aaacgacaat catcaaatta ctgatgcgtg aatacgatga
    28501 ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa gaatattcat tgaatgcttt
    28561 attaggatca atcggctatg tgcctcaaga tcacttcttg ttttcgatga ccattcgaga
    28621 taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt
    28681 agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga
    28741 acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat
    28801 caaaaatcct gaattgctga tcttagatga tgcactttct gcagtagatg ccaaaacaga
    28861 agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacca tcattgcagc
    28921 tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat
    28981 cattgaacga ggaacccatc atgagctgct tgcattaggc ggctggtatg aacgaatgtg
    29041 ggagaaacag aaattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat
    29101 caatcggaat ggacaaaatc gattccactt aaagaacaga agaagatcat caaacgactg
    29161 atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgcc
    29221 ctgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg
    29281 gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta cttgtttgga
    29341 gtcatagtca aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg
    29401 aagacctatc agtatgttcg ggtaagatta tttgaaaagc tgcatacact aggtatgcgt
    29461 tattttgatc agacaccggc aggatcgact gtttctcgag tgacaaatga cacagaaacg
    29521 ctatttgaat tttggtatgt ctttttgatg gtattgactg ggatctttgc agtcgtttct
    29581 tccttcattg ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg
    29641 ccgattttag gaatcgtcat ctggtattac cagaaattca gttctcgtat ttaccggaat
    29701 atgcgggaaa aattaagtca gctgaacacg aaattgaatg aatcgatttc cggcatgcgt
    29761 attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaac
    29821 gagtatttag acacgcgcta tgcgatgatc aaaacaaatt cgattctgtt aagtccgatc
    29881 atcaattttc tatatgcatt ggcgattgcg ttggcgttga ctttgtttgg gatcgatgcg
    29941 ctgcattctc ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt
    30001 tttaatccaa tgtctcaaat gatggacttt ttgagcacct ttacagatgg aatggttgcc
    30061 ggtagtcgta ttttgaaaat catggataca gaagaatatg caccgcaaca aaatcctgga
    30121 gcagatcaag cgattacttc aggtaagatc gagtttcgca atgtaagctt ttcttatgat
    30181 ggagagcaca atgtattgaa caatatctct tttgtcgcta atccgggaga gaccgttgca
    30241 ttagtggggc acaccggaag cggaaaaagc tcgatcatca acgtattgat gcggttttac
    30301 gaattttatg aaggccagat cttgattgat gatcatgaca ttcgagaata tccaatggaa
    30361 gagttacgta agaaaatggg attggtcttg caagatgcct tcttgtttta tggagatatc
    30421 gcaggaaata tccggatgat gaatccaact atcacagacg aacagattcg ccatgcagct
    30481 gagtttgttc aagcaaacaa gttcatcgat gaattaccaa aaggatatca ctcaaaagtg
    30541 atcgaacacg gtgccagcta ttctagcgga caacgacagc tgatcacttt tgcgcgaaca
    30601 atcgtgacag atcctaaaat actagtattg gatgaagcga cagcaaatat cgatactgaa
    30661 acagaaggat tgatccaaga aggattgaca aaaatgcgac aaggacggac aacgattgcg
    30721 attgcccatc ggctgtccac gattcgggat gcagaattga ttttagtttt agacaaaggt
    30781 catatagtag aacgtgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
    30841 atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg tgacagggct ccgttcagct
    30901 ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga tttttgcata aatttggctt
    30961 atcaccgata agctggtact ggaacaccgt atattagaag ttgggacagt agcatagtag
    31021 cattttgacc taagcgctag agtgaaaata aaacagagct tcccagattc ttcatctgtg
    31081 agcgttagtg tcgtaattac cagatttaaa aaaaatcacg acagctagtc agaagtgccc
    31141 aatagccgct aagaaagcgt tgacaaaagt gcttgctttc gataatataa agaaaacgat
    31201 acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggttccat
    31261 ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc atggaaagga
    31321 gtaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag
    31381 aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc
    31441 gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcacaaca
    31501 gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat
    31561 tgaatataca aaagaggctc tacaagtcca gttgaatgat tcggtgtata ttgcattgac
    31621 ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt
    31681 gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa
    31741 gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt
    31801 gcatttagtc aatgcacaag gcattcaaag ccaacaagac aatacctacc tgtttactac
    31861 aacaatacag gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga
    31921 ttcagtccat ttttttcgat tcgtcacaca cctacgtttt tttctgacac gcgtcctaga
    31981 tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg taccaggtta ttcaaaaaaa
    32041 atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg agatattcaa
    32101 ctaccagatg tccacggacg aaaaaatgta tttaacgatc catattgcgc gtttagtgca
    32161 tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt aacaagaacg catgaaataa
    32221 gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg
    32281 aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc ggcgggaagg aaaatatcaa
    32341 tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa
    32401 tgatgagatc ttgaaaaata tggacggtgt tgtgacggta atgcgagcgg gaggacagta
    32461 ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa
    32521 ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga
    32581 tggaatatcg ggctgtttcc agccattcct tgctattcta gctgctggtg ggatgattcg
    32641 tggactgaca gcattacttg tcttcttagg cgcatttgac cgcggttctg gcacatttgt
    32701 tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac
    32761 agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttgggg cagcattaat
    32821 gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccac tgactaccat
    32881 attttctggc actatttttg aagcacctat ttatcaaaca gtattcggca ttccctggat
    32941 tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt attgtgttgt tagctagcca
    33001 aattcagaaa ccaattaaaa agttagtacc tgagatgatc gcgaatttct tcgttccatt
    33061 cttcaccgtt cttattacaa tgcccttagg ttttttactc gttggaccag cattcacatt
    33121 cgcaactgat attttaatgg ctggatttga aacactttta gcactttcgc cagttattta
    33181 tggagcaatc gtaggcttct tctggcaaat tctagtgatg tttggtctcc attgggcaat
    33241 cgtaccaatg ggattgatgc aattttctgt gaatggttgg caaaatatca tgacacctgt
    33301 tgcagtagtg tcttttggac aaactgcagc acttactgca ctctacttta agcttagaaa
    33361 cccaaaagac aaagcaatcg caataccagc cattgtttct ggtattgtgg ggattacaga
    33421 gccagctatt tatgggttta cattgccacg taaaaaaata tttattttta cctgtgtcgg
    33481 tggtgcgtta gggggcgcgt atagtggatt aatgaattta acttcttgga atccaggtgg
    33541 attaggcatc tttacaattc ccaactatat tagaccagat ggtgatttaa cggatgtgat
    33601 taatgtattg attggaatcg ccattgcgat ggtggtatcc tttgcgctga cttttttctt
    33661 ttggaaggag gaagcaggtg aaacagatga tagacagaaa agatcaggta aagaaattgt
    33721 aaaaacaccg atacaaggac aaatcgctcc actgaatgca gcaaaggatg cagcttttgc
    33781 acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc
    33841 ttttgacgga acagtcatga ccctgttccc gacgaaacat gcaatcggaa tggtttctga
    33901 agcaggtctg gaattgctga ttcatgtcgg attggatact gttcagttgg aagggaaata
    33961 ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa ggcgatctgt tgctccgctt
    34021 cgacaaaaag aaaatcgaag aggaaggata cagtttagaa acacctgtga tcgtcaccaa
    34081 ttacacggat tatttggata ttttggaaaa tcaaggggag acggtcgggc cagacgatgc
    34141 acttatcacc gcactgacct gaacggttag gtgaaagaat ggactaggct taattattct
    34201 ttttagtcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    34261 gggcgtttat caaatttttg tcctacagaa attagatgga ctaaattctg ttgataattt
    34321 tctgcaaaaa cacaactata taacacgtct agaagataac aaatcgatgt attgatcgta
    34381 aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    34441 attgaagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    34501 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    34561 ccgcacatat cactcgtgca attgtacgct tcatagaacg attcaccaaa agtggtgaca
    34621 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    34681 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    34741 tgcagttggt catgatgaat gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa
    34801 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    34861 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    34921 acccagccat caaaacccat tttttttgct acacggatga gtgtggacgg gtggacataa
    34981 catcgctcag caatctcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    35041 aactgaatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    35101 aacatgtaga taccttcttc ttgttttttt acgtaaatta taactttttt gaaaatgatt
    35161 tgtaaacgga tttatgttat atagcttaat aaagacattt tttgacaaga tatttacaaa
    35221 aatccagtag aaaacactaa cttttgtaaa tgttactgga aagaaacgaa tttctattgt
    35281 ttgttttgtt tacaaaaaag ataataaaag tgtaaaaagt aaaggagtga gaacatggca
    35341 aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccccga gttgatggaa
    35401 ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt agatgtagaa
    35461 gaaggaaaag aaaagcttga gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct
    35521 ccgtatgaag tcaaaatcta tgcaactctg gatcgggaag aagcattgaa agatgcggat
    35581 tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc
    35641 cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat attcaaagct
    35701 tttcgtaccg ttccagtggt tttggcaatc gttgaagaca tgaagcgttt atgtccaaat
    35761 gcttggctga tcaatttcgc gaacccaagc gggatggtga cagaagcagt tatcagatat
    35821 ggcaaatggg ataaagtgat aggattatgc aatgtgccag taatggcgat gatgacagaa
    35881 ccaacaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg tctaaatcac
    35941 ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga attgattgat
    36001 cgtttatacg atgaagacaa tggattgccg aaaaatatct ttgatatccc gtttttcaaa
    36061 gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta ctaccgtgaa
    36121 gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag agcgcagcaa
    36181 gtgaaagaaa cagaagcaga gctgtttgaa ttatacaaag atccagaatt ggatcataaa
    36241 cctgaacaat tgcaaaaacg aggtggtgct tattattcag atgctgcctg tgaaacgatt
    36301 gcttcgatct atggaaataa agaaacacag atcgtggtat ctacaaagaa tgatggagct
    36361 gttcctgatc tgccggcaga ttgtgtagta gaagtcactg cctatgttgg cgcacaaggc
    36421 gcacgaaatg tagcgtttgg ttcccttcct gctgctgaaa gaggctggtt gcaagtaatg
    36481 aagaatatgg aattgctgac gatcgaagca gctgtgactg gtgattatgg aacagcttta
    36541 caagcgttta cgatcaatcc gcttatacga tcaggaaaga cagcaaaacg gatcatgaat
    36601 gagttgttta ttgcacatca gcagtattta ccgcaattca aggaaacaat cagccggtta
    36661 aaagcagaag gaatcgaagt ccaagatgaa ctggcaagag aattatcaag caatacacat
    36721 gtaatggagg aagtatgatg ggattcagaa aagatttttt atggggcgga gctacagcag
    36781 ccaatcaatg cgagggcggt tatgatgaag gcggacgtgg gttagcgaac gtagatgttg
    36841 tgccaatcgg agaagaccgt tttcctgtca ttactggaaa aaagaaaatg tttgaatttg
    36901 atgaaaatca tttttatcca gcaaaaattg cgatcgatat gtatcatcat tataaggaag
    36961 atattgcttt atttgcagaa atggggttca agacttatcg gatgtccatt gcttggagtc
    37021 gtatttttcc aatgggcgat gaaaaagagc caaatgaatc tggtttgaaa ttttatgagg
    37081 atgtctttaa agaatgtcaa aagtacggga tcgagccgct tgtgacgatt acacattttg
    37141 attgtccgat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt
    37201 tttatgaaaa tctgtgccga gtcatcttca atcgctataa aggacttgtc aaatattggt
    37261 tgacattcaa tgagatcaat atgatccttc atgcaccatt catgggcgca gggctgtatt
    37321 ttgaagaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat gagttggttg
    37381 caagtgccat cgcaacgaag attgctcatg aagtagatcc ggagaacaaa gtaggctgta
    37441 tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt tgggcttcta
    37501 gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga gaatatcctg
    37561 cttatgcatt gaaagaaatg gaacgaaatg gcattcagat caaaatggaa cctggggatg
    37621 ccgcattgct gaaagaacat actgttgatt ttatctcctt ttcttattat tcttctcgag
    37681 tttcgactac tgacaacgaa ctcttggaac agactgcagg aaatatcttt gcttctgtca
    37741 aaaatccata tctggaagca agtgaatggg gttggcaaat tgatccatta ggcttgagaa
    37801 taacgatgaa tgatatttat gatcgttatc aaaaaccact atttattgta gaaaacggct
    37861 taggtgcagt cgatacgccc gatgagaatg gttatgtgga agatgactat cgaatcgact
    37921 atctggcagc acatatccag gcaatgaaag atgcagtaga attagatgga gtcgatctat
    37981 taggctatac gacttgggga tgtattgatt tagtatcagc tggtacaggt gagatgaaga
    38041 aaaggtacgg attcatttat gtcgatcgtg acaacgaagg aaatggcaca ttagcccgtt
    38101 cgaagaagaa atcattcaac tggtataaaa aagtaattga ttcaaatgga gaaaatcttt
    38161 cgaatgattg atcaatgatt tataaaagtc tattgttttt cagcggattt ttcgagtata
    38221 ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt ggctgtaaag
    38281 tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc gttattggcg
    38341 caggtcctgc tgggatgact gctgctttat atgcttcacg ttcaaactta tccgtattga
    38401 tgatcgaaca aggcgcccct ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc
    38461 ctggtttcga ttcaataatg ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa
    38521 gatttgggac agaaaatgct tatggaatcg tgcaagatat caaggatcac ggcagccaca
    38581 aagaagttat ttgtgaagat aaaacttacg aaggaaaaac agtcattatc gcaacaggct
    38641 gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacgt ggcgtttctt
    38701 actgtgcagt ttgtgatggt gcgtttttcc gtaacaaacg tctagtagtg atcggaggag
    38761 gagactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa gtagtcatcg
    38821 tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg
    38881 aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttgggaat gaaatggtag
    38941 tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg
    39001 gagtattcat ctatgttgga ctggatccat taactgcacc atttagagaa gcagggttga
    39061 cgaacgaagc tggctggatt ccaacagaca acgaaatgaa gacgaatata ccaggcgttt
    39121 ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactactgct gttggtgatg
    39181 gtgggatcgc tggacaacaa gtgtataaat acttggaatc tttagaagaa acaactgcat
    39241 aagtttaaaa agagtggaag aacggtttaa ttgttctttt actctttttt ttcgcgtgaa
    39301 atctactgaa tccgatttat gcgaatactt gcaaccgtgc ttgataaaag aatcctttct
    39361 tgatttaagc gttttttggt cctcatactt tcatatctaa taagtgaatg ctatacttat
    39421 taagaaccat atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca
    39481 atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga
    39541 agatcctgaa aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggcat
    39601 gcggggaatc ttagggcctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac
    39661 agaaggactt gcacgtttca tggatacaca aggcgaagaa acaaaacgcc gaggtgtcgc
    39721 tatcgcttat gactcacgcc atcaatcacc agaatttgcc atggaagcag ccaaaacatt
    39781 agcacatcac aatatcccat cttatgtatt cgaaagtcta cgtccaacac cagagctttc
    39841 ttttgcagtc cgtcatttga agacgtttgc gggaatcatg gtaaccgcat cccacaatcc
    39901 agcagcttac aacggatata aagtctacgg agaagatggg ggacaaatgc ctcctgcaga
    39961 cgcagatgca ttgacgaaat atgtccgtga agtcagcaat ccgttaaaag ttgaagtatt
    40021 atctgacgaa gaagctgaac atagcggatt gatcacgatc atcggagaag aagtggatgc
    40081 cgcttatcta gaagaaatca aagcagtaac gattgatcat gaattggtgg aaacaatggg
    40141 gaaagatttg aaacttgtgt acacaccact tcacggaaca ggaaaaatgt tgggcgaacg
    40201 tgcgttgaaa caagctggat tcgaacaatt cgtactagtt ccagaacaag ctgtcgcaga
    40261 cccagatttc actacagtca aatcaccaaa tccagaagaa cactctgctt ttgagtatgc
    40321 gatacgactt ggcgagaaag aaggcgctga tttgctgatt gcaacagatc ctgatgcaga
    40381 ccgtttaggt gcagccgttc gtttgccaaa tggctcctat gaagtattga ccggaaacca
    40441 aatcggagca ctgtttgctc aatatatttt ggaagcacac caatcgcgag gcactttacc
    40501 tgaaaacgca gcggtattga aatcgatcgt ttctagcgaa ttacctacag caattgcgga
    40561 acactatggg gctaccatgt tcaatgtatt gacaggattc aaattcattg ctgaaaaaat
    40621 tcaacaattt gaagaagatc attcccatac ttttatgttc ggattcgaag aaagttacgg
    40681 ttatttagtt aaacctttcg ttcgcgacaa agacgcgatc caagcattgc tcttattagc
    40741 agaagtagca gcttactata aaaaacaagg caaaacgttg tatgatggct tgcaaacgat
    40801 ttttgaaacc tatggctact acgcagaaaa aacgatttct gtgacaatga gcggacaaga
    40861 aggttctgcg aaaatctctg ctttgatgga aaaattccgt gaagaagcac caacagaatt
    40921 tgccggagca aaagtcatcc aaacagaaga cttcaaagag ttgacgagaa cagaccgtga
    40981 aggaaacgta gaaaaactaa cgacaccgcc atctgacgta ttgaagtata cgctggaaga
    41041 aaatggctgg atcgctatcc gtccttcagg aactgagccg aaaatcaaat tttatatcgg
    41101 tgtaaaagga aactcttcag aagacgcagc tgaaaaagta gccgcttatg aaacgattat
    41161 ccaaaagatg acagaagaat aaaaaaactt gagactgatg gaaaaagtgg taaactttag
    41221 aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa aaacaaaagt
    41281 agagaaagtc agccaactat tcaaaatgtt gagtgacccc actcgtctca aaattctgct
    41341 gtatctgaaa gacggggaac aaaatgtgac agcaatcatc caagcagtgg aaatggaaca
    41401 atctgctgta tcgcatcaac tgcgtttatt aagagaaaac catgtcgtca agtcccatcg
    41461 tgagggaaaa gcgattttgt atagtcttga tgattctcac gtactggata ttttgaacca
    41521 gacactcaaa catgtggaac atcaataacg ataggtgcaa atagagacaa actggactgt
    41581 gtcaacgaat cttcttcgct gacacagtcc agttttttta aagaaaaatt gttagtctgt
    41641 ttcttcggtg gtttcagttc cataataact atggtcttct aggtctaaag cattcaaaat
    41701 aagcgaagct gtttcttcaa tagataaaga agcgacattg attactacgc agcccaattt
    41761 ttcataaaga tcattcgcaa aatcgagttc agctcgtatt ttttcgatat cggaataagc
    41821 agtatcagga tttaatccgt aagagcgcat ccgttcttta cggatattgt ttaaaatatc
    41881 tgggttattc gtcagtccca cgattttttt aggatcgatc tcccaaagct gtttgggaat
    41941 atgtgcttgt gggatcaaag gtagattggc aacttttaaa tttttgttag ctaaaaacaa
    42001 gctcaaaggc gttttggaag ttcgggatac gcctagcaaa acgacatctg cttctagaaa
    42061 cccacgagga tctttcccgt catcgtattt tacagcaaat tccattgctt tgatccgttt
    42121 gaaatagttc tcattcaata aatgaagggc gccaggttgc cgaatgggct tgataccagt
    42181 taagcgctcg atttctgata tcggcggagt catcacatca aaactaaata attgatgttc
    42241 ttcacaaaaa gaacgcgtca tctcgatcaa ttcttgattg atcaatgtgt gcaggacaag
    42301 agctttttct tctagtgcat cttgcagtgc ctgcatcaat gttttctcat ctgtcacaaa
    42361 tgttctgcgg aataaattga actcaacgct aggatattgg gccatagtgg cttgcgctag
    42421 ttttgtcgca gtttctccag cagagtcgga gatgacaaaa aaagtaacga tttctgtaga
    42481 atcggaagac atggataaaa cctcacttgc atttttttta tgtttattat accatactct
    42541 agaatgtgta cttcgtaatc attttgattt ggaggaataa gtcatgaaaa aacagacgtt
    42601 gatcgaacaa tcactagaaa cattgaaaaa taacggtttt aaatatacta aaaaaagaga
    42661 agcattgtta acttatttag tgaagcgcaa tcgctacgta tccgctaaag aagtttttga
    42721 ctatatgaat caggaattca aaggagtaag ttacgatacg atttatcgaa atctccatga
    42781 ttttgcagaa atcgatttac tggaagaaac cgatttaaat ggagagatga agtttcgttt
    42841 tcattgctgt caaggggaaa tcggccatca ccatcacttt atctgtacgg tgtgcggaac
    42901 aacgaaagaa atccatatgt gtccaatgaa ttatttcgaa gaacagctcg gtggatgtac
    42961 gatcgaaggg catagattcg agattttcgg aagatgtgaa aattgtcaat aaggatttaa
    43021 ataaaataac atttgataat tttaaaaaaa agcaagattt ccttgacggt caaaacagca
    43081 ttagatataa tatttacagg acagtcttgt tttttgagat atatttcaat aacaattacg
    43141 gttacttttt aagctcggag ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac
    43201 gaatcacttg acgatgctct tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta
    43261 caagaatctc gtaaacgtga attctacgaa aaaccaagtg taagacgcaa gaaaaaatct
    43321 gaagcagcta gaaaacgcaa aaaattctag ttctgtgatg aattggagtt ggtagtgtgt
    43381 cactactaag tagattaaac gatgacatga aaacagcgat gaaagcgaaa gataaggaat
    43441 ctttacaagt catccgaatg atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc
    43501 acgacttgac agaagaagaa gaattaacgg tgttgtctcg cgaaatgaaa cagcgacgtg
    43561 attctttgca tgagtttgaa gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg
    43621 aaattgtgat agtcgaaaaa tacatgccgg aacaactttc cgacgaagaa attcgacaac
    43681 ttgttcaaga agcaatcacg caaactggtg cttcatcaat gaaagaattt ggcaaagtca
    43741 tgggtgcgat catgcctaaa gtcaaaggaa aagcagatgg aaaccaagta aatgccattg
    43801 tgaaagaact attacaatct taattttatc atttaaactg cggacaatca ttgtccgcag
    43861 tttttttgtg ctcgtaaaaa agagatctaa aaaatagcta gacgaaagaa taggattctt
    43921 tcatctagct gtttttttcc tgtttaatat ttcttatgtt aaattgtgtt tgatctggtt
    43981 attcttctgg tagttcattt gcttcttgtt tataccctga taggcgatag gcgtaataga
    44041 agagtaagct gccagcatac ccaagtaacg ttgggaccat ccagccaagt cccaatgcag
    44101 caaaaggaac gtgatgatga taaaattcga gcatgcttgc tacaactctt tgttgactca
    44161 ctaaagcagg cgaatttgcc agcatatcca atacggcggg aacagcagca aacaacatcg
    44221 ttacttgata gacaaatggt gtcttttgga aaaattttgc tgttaaggaa agcaaaatca
    44281 acgcaagcga caatggatac aaaagcatca atactggtaa agaccattgg atgatattgt
    44341 ccaaaccagc atttgcaacg acaaaagaga cgaaagtcgt taaccgcagc catgtcatat
    44401 aactgacttt agggaacagt ttatggaaat cttgtgcaaa agaaacaacg agtcccatag
    44461 cggtagtgaa gacacctaat gtgaccatca ctcctaagaa gctagcgccg aagttaccta
    44521 aataaaaatt agtgatctga gaaagtgcag ttccgccatt ttctgataaa gacatcttat
    44581 tcaaactcat cgcaccgaga agaacaagac caaaatagat catgacttct agcgcaacgc
    44641 tcaggctacc agcttttgct gttaattctg tgactttttt atctttgaaa ccaaggtcac
    44701 ggactgcatg gacaaaagtg acacttaagg caagcaaggc tactgcatca accgtgttat
    44761 aaccttctag gacaccattt gttaaagcgt ttgtttggta agtatctgca gcagattgat
    44821 ttagatttcc cattgggtgg aataaagaaa cgacaaaaat gattgctaat aatgcaagaa
    44881 acaaggagtt tagatacttt ccgacgtatt tgaccaaccg tgtgggatgg acagtaagga
    44941 aataagcaag taaaaagaaa agagcagaga ataaaaacat acctaatgaa ttatattttt
    45001 caggcaaaaa tggtttagca gccatttcaa aagcagtagc agctgtccgt ggtgtaccga
    45061 aaaatggtcc gatcgtcaaa tgaacaagga ttaagaacaa ggcagcatac cattttccga
    45121 caggtttagc taagtcatac agaccatcac tttttgtcac tactacagct aagatagcta
    45181 gtaatgggaa aagagcgcct gagatggcaa atcctacgcc agcactgacc cagtttgcgc
    45241 cagccatttg acctaagtgg acaggaaaga ttagattacc tgacccgaaa aacatcccga
    45301 agataagtga actgaccatc aatagttgtt tccatgaata agtttttgtt tcttccataa
    45361 atttttcctc caataattat cattttgatt gacgatataa acgttgcagc aattagtaat
    45421 gccactaata tcagattcca tgaaaaccac caccttaaaa tataaaaaaa agcccttttc
    45481 cacttgggaa aagggctcta gattctagaa cggtaccacc ttttttcgct taaaaaagct
    45541 tcttcattca cggacgcttt ttgataaagt tatccgctag cctgataatg gaggcagcca
    45601 caatgatcta cgttttcgac attgactcgg aaatgatgtt cgtctattcg atttattgtc
    45661 atttctcact tacatgactc actgggaata aatcgttgaa tagcctactg ttttcctcga
    45721 cgctttgctg tatgtttatt tgttgacgta ataatacacc tgtttttttt gaaatgcaag
    45781 aatattttaa ctattttgtt ttcttttttt gaaacaagaa aaatacctag agaatacctc
    45841 tgaatgaaac ggaatatttt gttctccact ttcatataac ttacattctc agattctttt
    45901 tatcaaaacg agacctttag cgaatggaaa cagctatttt ttaacgtttc ttggtacgta
    45961 tggttaaacg actcttttac aaccttttct tatttgctat atagtttctt ctgagtatgg
    46021 taaaatagga aagaatgaat cgaaaaaaag gggatgtcta tttttgacgg aagagtctag
    46081 ttctttagag attagattga cagctgctga tgatgtcagc atgcttttag gtgcacacga
    46141 caagcatata aaactgatcg aggaaacaac ggaaacaacc atccatacac gtggagaaat
    46201 cattcagatc attggtgaac aatcccagat ttccttggct gcctcagtca ttcggacatt
    46261 acaggaattg atcaaacgag gaatccacgt aagtacacca gatgtcgtga cggcattgaa
    46321 aatggggcaa aaaggtacat tagattattt tgttgagatg tatgaggaag agatcgtcaa
    46381 agaccgtaat ggaaaaccta tacgggtaaa aaacagcggt caaaagaaat acgttgaatc
    46441 ggtagccaaa catgacatcg tatttggtgt agggccagcc ggaacaggga aaacgtttct
    46501 tgccgtagta ttagcaattg ctgccctaaa aaaaggcgaa gtgcaaaaaa tcattttgac
    46561 gcggcccgct gtagaggcag gggaaaacct aggctttcta cccggggatc tgaaagaaaa
    46621 agtggatcct tatctgcggc ctgtttatga tgctttatac caaatttttg gattggatca
    46681 tacgaaccga ttgatggaac gaggcgtaat cgaaatcgca ccattagctt acatgcgggg
    46741 gcggacattg gatgatgcat tcgttatctt ggacgaagca caaaacacga cagtcgctca
    46801 gatgaaaatg ttcttgaccc gtctcggtta tcaatcaaaa atgattgtca atggggatac
    46861 aagtcagata gacttgcctc gagggacgac aagtggtctt gttcatgcag aacgaacact
    46921 aaagcaaatc aaaaaaatcg attttgttaa ttttgaagca agtgacgttg ttcggcaccc
    46981 tgtagttgcc gaaatcatca aagcttatga aaaggcggat cttcatcaag aataagcttt
    47041 gcttaggagg ataagcacat gttgaataaa acactgcaga aactacaaga aaaactcgga
    47101 aaggcagcat tgcctttcct tctgttcttg ttttttatca ttgtctgcgg cgttacattt
    47161 agtagtgtca aacaaaaggc caatgatttc agagaaggcc aagtagcaga agagagtatc
    47221 cgtgcgaata aaacgatcga gaacacggaa gaaaccgaac aaagaagaaa actggcagca
    47281 gaagcagtga ctccagaata tacttatcaa aaagatctgg cagatgacca gaacaatcgg
    47341 atcaagcaat tattcgaatt gatcgataag acgaatgata ccatcaacaa atcttataat
    47401 gacaaggtag ataaggcgaa ggaagatgaa aatatcccga aacctactac agaagagcgg
    47461 attgctacac tgaagaaaaa ttttgaaaac gtgaatgcag aaaacgttgc gttttaccaa
    47521 aaactgccga acaattttta ttcgacgata tttgaaatga ctgaaacgga aattcacacg
    47581 gttagggatg aaagtttgaa gctcattgac gaacagatga gtaaacaagt tcgtgaaagc
    47641 gaattggaag cttttaaaca agaagcagaa gatcagattc agtatttgaa tgtcacaaca
    47701 gagcagcaac aaatgattcg ctacttagtg gatcaaggca ttgtagtcaa tgatgtattg
    47761 aatgagaaaa agacagaaga attaaaacaa tctgcacgag aagctgtaca accagtgatg
    47821 atcttccaag gagaaatcat tgttcgcgaa ggaaatcaaa ttgatgcttc agcgatgaaa
    47881 aaattggaat tactaggatt gacgaatcaa acgacttcga ttttcccact cattgccatg
    47941 gttttagctg ttttgcttca aatcggcgta ctgatttata attcacttca attccaaaat
    48001 gcaggagaaa gaacaaaata cgtgcttttc tatgtgacag ctatgtcagt cagcatagtg
    48061 ctgatgaaat ttttccaatt attccaaacg gaacaagcta cttatatccc gctgttttat
    48121 cccgctgctt ttgcaccact tgttttaagc tttttcttga accgaagagc agggatcatg
    48181 gctggtattt tccaagcagt atctgcactt ttcatttttt atgattcgat tggcacgaac
    48241 tttctaacga ttatcttgat gtcttatgtt ttctctgggc tcatggcaac gattgtcaga
    48301 cgcaaacgga tctcagaaca aggattatct gctgccatgt gggtcattat ttttcctgtg
    48361 atgatggatg tcattttgat cgtctatcaa ggaatgagct ttggtgatgc aacgacttgg
    48421 ttaatgcttg tctgcgggtt cactggtgca ctcttctcat tcttgctgac gatcggtcta
    48481 cacccatata ttgaattgat ggtcaatgat gacagtgtaa ttgtattaaa tgaactcagc
    48541 aatccaaatc atcctttatt gaaacagttg ctggaagaag cgccaggtac ttatcatcat
    48601 agtatgatgg tagctaatct aagtgctaac gctgtagcag aaatcggcgg acgttctttg
    48661 ctaactcgag tagcttgcta ttatcatgat atcggcaaga tcaaacatgc tagctttttc
    48721 gttgagaact tgccttcagg agcggaaaac ccgcacaatt tcttattgcc agaagacagt
    48781 aaacatatta tctttggaca tgtgacagat ggagccaaaa tattggaaga ttataatatg
    48841 ccgcaaatgg tgatcgatat ttgcaaacag catcatggaa ccacgctaat gcgttatttc
    48901 tacatcaaag cgaaagaacg caatccggaa gtaacagaag agcaattccg ttatcctgga
    48961 ccaaaaccgc aaagtcgtga agcagggatc gtcaatattg ctgacagttg tgaggctgca
    49021 gtacgggcaa tggatcatcc gacgagcgaa aaaatcgaaa gttttgtcca taatcttatc
    49081 gaagagcgta tttccgatgg tcaattagac gatagtggac tgaccttgaa agaaatccga
    49141 aaagtggaaa aatcattgat cagcggttta agtagcacct tccattctcg gatcaaatat
    49201 cctaaaatga aaacagaagt tgagaagata aaggaagaac aagaagaata gaaagaagcg
    49261 atgtataatg gatattacct ttatggatga aacaaaccaa gtctcagaag agaaaatgaa
    49321 agaaatcgat gacttgctgc aatttgcagc atcttattta aagctgccag aggaaacaga
    49381 aatgtctgtt actttcatgg acaatgcggc aatccaagta atcaatcgtg attaccgtgg
    49441 aaaagatgca ccgacagacg tcatcagttt tgctctggaa gaagaaggcg aagacgaaat
    49501 cccagtgatt tttgaagatg gagagaatcc tttgcctcgt gtactaggag acatcatgat
    49561 ttctattgaa cgtgctgctg aacaagcaga agcttacggc cattcatatg atcgggaact
    49621 tggcttttta gcggtacatg gattcttgca tatcaacggg tatgaccata tgacaccaga
    49681 agatgagaaa gaaatgtttg gtttacagaa agagatcttg gatgcctatg gactcaaaag
    49741 ataaaaacgt agagaaaaac aaacatttcc tgatgtctgt ggaatttgct gtccaaggag
    49801 tcaaaaccgt ttttgaggaa gaacgcaata tgaggaaaca tgttgcgttc ggtatccttg
    49861 ctttatttat gggcgtgatt ttccagcttg atcggataga gtggttatgg ctgcttttag
    49921 ctgtttttct cgtttggata gtagagatca tcaatactgt ttttgaaaat gtagtggaca
    49981 tgtttacgga ttttcatttt catccgattg ggaaaaaaat caaagatatg gcagctggag
    50041 cagttttagt gaccgcttgt tttgctgtca tcattgggtt gattttattt gtccctaaaa
    50101 tatatcattt gttgtttaat taaaaaggag aacgtatatg ccagaacata aatcagggtt
    50161 tgtcgcaatc gtcggccgcc cgaatgtagg aaaatccaca ttattgaacc gaatcgtggg
    50221 tcaaaaaatt gccattatga gtgacaaggc acagacaact agaaataaaa tccaaggagt
    50281 atatactaca ccagaagctc agctgatttt tatcgataca ccaggaatcc ataaaccaaa
    50341 gcatcgtcta ggagatttta tggtagaagc agcttacagt gcgttgaaag aagtggatgc
    50401 agtcttattt atgatcagtg ctgatcaaaa acgcggccgg ggagacgatt ttattattga
    50461 acgtctaaaa aacgtgcatt ctccagttta tctgattatc aataaaatcg ataagatcca
    50521 tccagacgag ttgctgggaa tcatcgaaga ttattcgacg cagatgcctt ttgcacaagt
    50581 gattccgatc tctgctacag aaggaaacaa tgtcgaacgc ttaatggatg tattagtcag
    50641 tgaaatgcca gagggcccac aatattttcc agatgatcaa gtgactgatc atcctgaata
    50701 ctttatcgtt tctgaattga tccgtgagaa agtcttgttt ttaacacgag acgaagtgcc
    50761 tcattctgtc gcagtcgttg ttgattcgat gaagcgaaac gagaatgata aaatccatat
    50821 ccaggccacg atcatcgtcg aaagagatag ccaaaaagga atcattatcg gcaaaggcgg
    50881 aaaaatgctg aagcaagtag gaacgaaagc aagaaaggat atcgaaaatc ttttaggaga
    50941 caaagtattt ctagaacttt gggtcaaagt ccaaaaagac tggcgagaca aaaaagtcta
    51001 tctgcaagac tttggctacc gaaaagacga atactgatat tctgaacttc ttataggtta
    51061 agtatagaaa taaactggat aaaaatccgc acgttgagaa aaacggcgga agatcgaaac
    51121 ataaataatc tggatcggca gaatctgctt ttccagattt attttttagg agggagaata
    51181 tgagccagct tgcagaatcg aaaggcttga ttttatctag ccgtaattac aaagaaaaag
    51241 ataaattagt caaaatcttt acggaatctt ctggcaaaat gatgttttat atcaaaggaa
    51301 tccatcggca aaatcagcca ttagctccgg ctttgctccc ctttaccgaa gcagtctata
    51361 tcggtaattt tcgaagtgaa ggtttgtcgt ttttaagcag tgtcaaagac gtccatgcgt
    51421 ttcggaacat ccaagaagat atctttttga gcggttatgg tacctatttg ctaaatctag
    51481 tagatgcagc aatcgaagat catcaatatg acccgcattt attccaattt acgcaacaag
    51541 ctctgcagcg gatggatcaa ggagatgatg cagaaatcat cacgaatatc tttgaagttc
    51601 aaatactgca acgcttcgga atcgcgccaa tttggacgca ctgtgtcgta tgcggggaaa
    51661 caaaaggaaa atttgactat tcttccaaat ctggcggtgt gatatgcgag aaacactggc
    51721 caatggacga acaccgttat catgcagatc ctcgcgctgt ttatttcgtt cgaatgtttt
    51781 cggctatttc ttatgataag atcagtggaa tccacttgaa agaagaaacg aagcaggcaa
    51841 tccgtgacct cattgaccag ttgtatgatg aatatgtcgg tattcatcta aaaagcaaaa
    51901 agttcattga tcaaatgaag tcttgggcac acgtattaaa gacgccagag aaaaatatgc
    51961 ctgaaaaaga gatacctgaa aaagaagaat aaatgaaaga tacaacaaaa aaatcaaaac
    52021 tatttgacaa atacagggac ttccgtctat tattaaccgt agattaaata gatcgttcac
    52081 tgagaggatc tattatataa acaagaaaac tgcaaaatga atacgtacat tgaccaagat
    52141 cagtaaaaaa cacgtggttt agcgagttcg ggagagtgga agccggaccc aaacgatttt
    52201 tgaagaggca cttgcgagta cgataaatga agctgccgaa tctttattcg gaagtagggt
    52261 ggaaccgcga aattattcgt ccctatgtct gtaatcagac atagggactt tttgtcgttt
    52321 tctttcctgt attcttattg acagcaacgc ttaaaaattg aaaggggcaa aacagatgag
    52381 taaaaaattg accgtccaag atatgatttt aacattacaa aaattttggt catcaaacgg
    52441 ctgcatgctg atgcaagctt atgacacaga aaaaggagca ggtacgatga gcccatatac
    52501 attcttacgg gcaatcggac cggaaccatg gaatgctgct tatgtagagc catcccgccg
    52561 accagctgac gggcgttatg gagaaaaccc aaaccgtttg tatcagcatc atcaatttca
    52621 agtcgtaatg aaaccttcac ctgaaaatat ccaagagctt tatttagaaa gcttgcgtct
    52681 tttaggaatt gatccattag aacacgatat tcgttttgtt gaagacaact gggagaaccc
    52741 atcgatgggg tgtgccggtg tgggttggga agtatggctt gatgggatgg agatcacaca
    52801 attcacgtac ttccagcaag tcggaggact accttgtaaa ccagttactt ctgagatcac
    52861 ttatggacta gaacgtcttg cttcatatat tcaagaagtt gaaagtgtct atgatctgga
    52921 atgggctgat ggtgtgaaat atggcgagat cttcaaacaa ccagaatatg aacattccaa
    52981 atattctttt gaagtaagtg accaagactt gctactaagc aactttgacc gttttgaaaa
    53041 agaagctaaa cgctgtatcg atgaaaatct tgttcatcca gcgtatgatt atattttgaa
    53101 atgcagccat acattcaatt tattagatgc tcgcggtgca gtatccgtaa cagaacgagc
    53161 aggttatctt tcccgtatcc gcaacatggc tcgtgctgta gccaaaatct ttgtggcaga
    53221 acgcgaaaaa ttaggattcc ctttattgaa taaaaacaca gcaaatccag aaaacatgaa
    53281 ggaggctgaa taagatggca aaagacttat tacttgaaat cgggctggaa gaaatgcctg
    53341 ctcatgttgt cacaccaagc atgaaacaat tagaagaaaa aacagcaaaa tttttagatg
    53401 aaaaccaatt gacttatgat acgatcgaaa cgttttctac accgcgccgc ttagcaatca
    53461 aagtaacaaa cattccagaa cgtcaagcag acagcgaaga agaagtcaaa ggaccagcca
    53521 aaaaaattgc tttggacgca gatggaaact ggacgaaagc agctgaagga tttgtccgcg
    53581 gacaaggtct gacgacagat gctatcactt ttagagaatt aaaaggcgtg gaatatgtct
    53641 atgtaacgaa atacactcac ggcaaagaag caaaagaagt attgacaggt ttagcagatg
    53701 tcattaaaag cctcactttc ccagtaacga tgcactgggc agaatacgat tttgaatata
    53761 ttcgccctat tcactggatc gtggcattgt tagatgacga aataatcccg tttgagatct
    53821 tggatgtcaa aacagacaga gcttcaagag gtcatcgttt cttaggcgaa gatgtgacat
    53881 ttacgcatgc aaaagaatac gaagaaaaat tgaatgaaca atttgttatt gcttcaccgc
    53941 aaaaaagaaa agaaatgatc atcacacaag caaacgaatt agccgaaaaa aatcactgga
    54001 tcattgatct agatgaagat ttactagaag aagtcaacaa tttagtcgaa tatccaactg
    54061 tcttttctgg cgacttcgaa gaaaaatatc tatctgttcc tgaagaagta ctagtcactt
    54121 ctatgaaaga acatcaacgt tatttcgaag tacggaacga tcaaggtatg ttgcttcctc
    54181 attttatttc tgctcgtaat ggcgatcggg taaaattgga aaatgtggta cgaggcaatc
    54241 aaaaagtatt gacggctcgt ttagaagatg cggaattctt ttataatgaa gacaagaaag
    54301 cttcaatcga ccaatacgtt gaaaaattaa aaactgttac tttccatgaa aaaatcggta
    54361 gcatctatga gaaaatgcaa cgggtacaag tcatcgctcg catcataggc gaacaagtag
    54421 gactatctga ggaagaattg atggatctgc aacgcgctgc tgaaatctat aaatttgatc
    54481 tagtaaccaa catggtagga gaattccctg aactacaagg gatcatggga gaaaaatatg
    54541 ctctgctgaa aggcgagaaa ccagccgtag caacagcgat ccgtgaacat tacatgccta
    54601 tatccagtga aggagattta cctgagacta cagtaggttc tgttttagcg attgctgata
    54661 aattagatag tatcttttct ttctttgcag ttgggatgat cccaagtggc tcaaacgatc
    54721 catatgcttt gcgccgtcaa gcatatggtg tcgttcgaat catcgaatcg aaaaaatggg
    54781 cctttccttt gtctgttctg caagaaacga ttagtgaagt tatcagtaaa gatacggatc
    54841 gttttggcat tggcttgtca gcgggacaac aacaggtgat cgactttatc aaaggccgtc
    54901 tgcgccaatt attgacaacg aagaatatcc gtcatgatgt catcgaagct gttttgaatg
    54961 cagaacaaaa agatctgaca aaagtatttg cagcagcaca attgttcaag cagcatcttg
    55021 cagatgaaga cttcaaacca tcgatggaag ctttgacacg tgtcgtgaac ttagcgaaaa
    55081 aagcggagct tgaaaaacaa tcagaagtag accctgaact gttcgaaaac gaagcggaga
    55141 aagaactgca caaagcagta gaggcaatga aaacaagctt tgctgaccaa acgatcaacg
    55201 aaaattatga tcagcttgta agccttcgcc cactgatcga aaattatttt gatcagacga
    55261 tggtcatggc agaagaacca gcagtacgta agaaccgatt aacgcaatta agtcaaattg
    55321 ctgccatggc tttgtcacta gcaagtttag atcaaatcat cacaaaataa tttctaatat
    55381 gtaaataaac cagagaacag gcacaaaata g
//
LOCUS       DADVXN010000006        53654 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.006, whole genome shotgun sequence.
ACCESSION   DADVXN010000006 DADVXN010000000
VERSION     DADVXN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 53654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.006"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            22..1653
                     /locus_tag="KB942_000300"
     CDS             22..1653
                     /locus_tag="KB942_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBB6850755.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1715..2356)
                     /locus_tag="KB942_000301"
     CDS             complement(1715..2356)
                     /locus_tag="KB942_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6850756.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2542..2618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2638..2946
                     /gene="rplU"
                     /locus_tag="KB942_000302"
     CDS             2638..2946
                     /gene="rplU"
                     /locus_tag="KB942_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBB6850757.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2973..3317
                     /locus_tag="KB942_000303"
     CDS             2973..3317
                     /locus_tag="KB942_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBB6850758.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3330..3623
                     /gene="rpmA"
                     /locus_tag="KB942_000304"
     CDS             3330..3623
                     /gene="rpmA"
                     /locus_tag="KB942_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBB6850759.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4057..6864
                     /locus_tag="KB942_000305"
     CDS             4057..6864
                     /locus_tag="KB942_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6850760.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7055..7546
                     /locus_tag="KB942_000306"
     CDS             7055..7546
                     /locus_tag="KB942_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6850761.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7649..8638
                     /gene="manX"
                     /locus_tag="KB942_000307"
     CDS             7649..8638
                     /gene="manX"
                     /locus_tag="KB942_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBB6850762.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8671..9474
                     /locus_tag="KB942_000308"
     CDS             8671..9474
                     /locus_tag="KB942_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBB6850763.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9495..10409
                     /locus_tag="KB942_000309"
     CDS             9495..10409
                     /locus_tag="KB942_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBB6850764.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10492..10866
                     /locus_tag="KB942_000310"
     CDS             10492..10866
                     /locus_tag="KB942_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBB6850765.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11005..11250
                     /locus_tag="KB942_000311"
     CDS             11005..11250
                     /locus_tag="KB942_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6850766.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11422..12486
                     /locus_tag="KB942_000312"
     CDS             11422..12486
                     /locus_tag="KB942_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6850767.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12732..13157
                     /locus_tag="KB942_000313"
     CDS             12732..13157
                     /locus_tag="KB942_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6850768.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13150..13599
                     /gene="nusB"
                     /locus_tag="KB942_000314"
     CDS             13150..13599
                     /gene="nusB"
                     /locus_tag="KB942_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBB6850769.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13696..14550
                     /locus_tag="KB942_000315"
     CDS             13696..14550
                     /locus_tag="KB942_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBB6850770.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14551..15888
                     /locus_tag="KB942_000316"
     CDS             14551..15888
                     /locus_tag="KB942_000316"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBB6850771.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15893..16117
                     /locus_tag="KB942_000317"
     CDS             15893..16117
                     /locus_tag="KB942_000317"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBB6850772.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16119..17009
                     /locus_tag="KB942_000318"
     CDS             16119..17009
                     /locus_tag="KB942_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6850773.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17018..17836
                     /locus_tag="KB942_000319"
     CDS             17018..17836
                     /locus_tag="KB942_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBB6850774.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17943..18392
                     /locus_tag="KB942_000320"
     CDS             17943..18392
                     /locus_tag="KB942_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6850775.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18410..20092
                     /gene="recN"
                     /locus_tag="KB942_000321"
     CDS             18410..20092
                     /gene="recN"
                     /locus_tag="KB942_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBB6850776.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20147..20263)
                     /locus_tag="KB942_000322"
     CDS             complement(20147..20263)
                     /locus_tag="KB942_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBB6850777.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20336..21292)
                     /locus_tag="KB942_000323"
     CDS             complement(20336..21292)
                     /locus_tag="KB942_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6850778.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21463..21831
                     /locus_tag="KB942_000324"
     CDS             21463..21831
                     /locus_tag="KB942_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBB6850779.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22001..22432
                     /gene="mraZ"
                     /locus_tag="KB942_000325"
     CDS             22001..22432
                     /gene="mraZ"
                     /locus_tag="KB942_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBB6850780.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22453..23412
                     /gene="rsmH"
                     /locus_tag="KB942_000326"
     CDS             22453..23412
                     /gene="rsmH"
                     /locus_tag="KB942_000326"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBB6850781.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23417..23818
                     /gene="ftsL"
                     /locus_tag="KB942_000327"
     CDS             23417..23818
                     /gene="ftsL"
                     /locus_tag="KB942_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBB6850782.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23819..26011
                     /locus_tag="KB942_000328"
     CDS             23819..26011
                     /locus_tag="KB942_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6850783.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26037..26999
                     /locus_tag="KB942_000329"
     CDS             26037..26999
                     /locus_tag="KB942_000329"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBB6850784.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27013..28374
                     /locus_tag="KB942_000330"
     CDS             27013..28374
                     /locus_tag="KB942_000330"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBB6850785.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28400..29488
                     /gene="murG"
                     /locus_tag="KB942_000331"
     CDS             28400..29488
                     /gene="murG"
                     /locus_tag="KB942_000331"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBB6850786.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29503..30723
                     /locus_tag="KB942_000332"
     CDS             29503..30723
                     /locus_tag="KB942_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBB6850787.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30879..32204
                     /gene="ftsA"
                     /locus_tag="KB942_000333"
     CDS             30879..32204
                     /gene="ftsA"
                     /locus_tag="KB942_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBB6850788.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32227..33468
                     /gene="ftsZ"
                     /locus_tag="KB942_000334"
     CDS             32227..33468
                     /gene="ftsZ"
                     /locus_tag="KB942_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBB6850789.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33485..34162
                     /locus_tag="KB942_000335"
     CDS             33485..34162
                     /locus_tag="KB942_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBB6850790.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34176..34775
                     /locus_tag="KB942_000336"
     CDS             34176..34775
                     /locus_tag="KB942_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBB6850791.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34790..35077
                     /locus_tag="KB942_000337"
     CDS             34790..35077
                     /locus_tag="KB942_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBB6850792.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35126..35908
                     /locus_tag="KB942_000338"
     CDS             35126..35908
                     /locus_tag="KB942_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBB6850793.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35929..36636
                     /locus_tag="KB942_000339"
     CDS             35929..36636
                     /locus_tag="KB942_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBB6850794.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36898..39696
                     /gene="ileS"
                     /locus_tag="KB942_000340"
     CDS             36898..39696
                     /gene="ileS"
                     /locus_tag="KB942_000340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBB6850795.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39745..41271)
                     /locus_tag="KB942_000341"
     CDS             complement(39745..41271)
                     /locus_tag="KB942_000341"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBB6850796.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41286..41933)
                     /locus_tag="KB942_000342"
     CDS             complement(41286..41933)
                     /locus_tag="KB942_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBB6850797.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42117..42446)
                     /locus_tag="KB942_000343"
     CDS             complement(42117..42446)
                     /locus_tag="KB942_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBB6850798.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42623..43351
                     /gene="sfsA"
                     /locus_tag="KB942_000344"
     CDS             42623..43351
                     /gene="sfsA"
                     /locus_tag="KB942_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBB6850799.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43367..44380
                     /locus_tag="KB942_000345"
     CDS             43367..44380
                     /locus_tag="KB942_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6850800.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44380..45657
                     /locus_tag="KB942_000346"
     CDS             44380..45657
                     /locus_tag="KB942_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6850801.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45720..48422
                     /locus_tag="KB942_000347"
     CDS             45720..48422
                     /locus_tag="KB942_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6850802.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48574..48891
                     /locus_tag="KB942_000348"
     CDS             48574..48891
                     /locus_tag="KB942_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6850803.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48921..49241
                     /locus_tag="KB942_000349"
     CDS             48921..49241
                     /locus_tag="KB942_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6850804.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49349..50809
                     /locus_tag="KB942_000350"
     CDS             49349..50809
                     /locus_tag="KB942_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6850805.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50877..51098
                     /locus_tag="KB942_000351"
     CDS             50877..51098
                     /locus_tag="KB942_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850806.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51129..51311
                     /locus_tag="KB942_000352"
     CDS             51129..51311
                     /locus_tag="KB942_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBB6850807.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51311..51724
                     /locus_tag="KB942_000353"
     CDS             51311..51724
                     /locus_tag="KB942_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBB6850808.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51847..53028
                     /locus_tag="KB942_000354"
     CDS             51847..53028
                     /locus_tag="KB942_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6850809.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53099..53263)
                     /locus_tag="KB942_000355"
     CDS             complement(53099..53263)
                     /locus_tag="KB942_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850810.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53417..53490
                     /locus_tag="KB942_000356"
     tRNA            53417..53490
                     /locus_tag="KB942_000356"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53451..53453,aa:Arg,seq:cct)
BASE COUNT        17711 a         9107 c        11607 g        15229 t
ORIGIN      
        1 catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa aaaagaattt
       61 tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac agtagctgct
      121 gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt
      181 ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg tttttatcaa
      241 atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc aacaaaggga
      301 atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc agcaggactt
      361 ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg gtttacttca
      421 ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt tcactcggaa
      481 ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc gataaacaca
      541 gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg
      601 gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt tatgctattg
      661 ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag tagtaatcgt
      721 ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc agctattagc
      781 attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca
      841 aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa tctgttgctt
      901 cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa
      961 tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact gctctccatg
     1021 cttgtttttg cgccagtgat gtcacagttt ccaatgccag cacttgcagg tgttttgatt
     1081 gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac taataaatat
     1141 tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt tgatttaagt
     1201 attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct
     1261 atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga aacggaaaag
     1321 ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc ttcagaaaaa
     1381 ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc catgcgaggc
     1441 gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga aaaagcagag
     1501 caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa acaactttct
     1561 cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc gttgacagct
     1621 ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca gacaagactg
     1681 taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta
     1741 gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa atagctgctg
     1801 gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca tccttttgtt
     1861 ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta accgtatatt
     1921 ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat tttggctgga
     1981 ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa cgagtaggtg
     2041 tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga tgagtcacgc
     2101 gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt aagccagttg
     2161 aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa
     2221 tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga ggtccttgcc
     2281 cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct tgtatcagtt
     2341 cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata aaagaaacac
     2401 aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta ttgcttgaat
     2461 ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc tggtaagcta
     2521 gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa taagcacagc
     2581 atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct ttatagcatg
     2641 tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca agcaatctat
     2701 gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt tatcttagta
     2761 ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt tgaaggtact
     2821 gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc taaaaaacat
     2881 tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga cgcgatcaac
     2941 gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt aagcgaaatg
     3001 acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt ccttatggaa
     3061 gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac gggattgcct
     3121 cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt tatctttata
     3181 ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt ctattagaaa
     3241 atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc caaattaaaa
     3301 caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta caattatttg
     3361 cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca aaacgtctag
     3421 gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac cgtcaacgcg
     3481 gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa
     3541 aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt
     3601 atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg acaagacttt
     3661 tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg aagtttactt
     3721 tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt gataactatc
     3781 gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata tggtaaacag
     3841 tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc ttttaaattg
     3901 aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata gttgacgaaa
     3961 cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta tagaaaagga
     4021 gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga aaaaaccgct
     4081 catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc tgaagtgtta
     4141 gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg
     4201 ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac gcacgtaaag
     4261 ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga aaaaaataca
     4321 ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg cagcatgaaa
     4381 aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt aaattgtttg
     4441 attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta tcattttgca
     4501 aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg tgctgattat
     4561 gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg tgcttttaca
     4621 ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat gctgtttttg
     4681 gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt tatggatcat
     4741 ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt acggatcgta
     4801 ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg gcgtatccca
     4861 atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta
     4921 aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt gacagaagat
     4981 gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt gaagtcaaat
     5041 atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga aatctcgatc
     5101 acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc aagcaaccgc
     5161 agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc accaaatgat
     5221 acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata tgatatcatc
     5281 ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat caatcatttc
     5341 atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg tttttctttt
     5401 aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt gaccaatcag
     5461 atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt tatttatgct
     5521 atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga acggcatacg
     5581 aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt tgcgaaagaa
     5641 gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga agactattac
     5701 ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat cggtgtagtc
     5761 gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca acagttgtta
     5821 gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa aacagcattg
     5881 gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat actcttagta
     5941 gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg aattccagta
     6001 cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa ggtctcagta
     6061 ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa
     6121 atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa agctgttttg
     6181 gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat cgaacgagcg
     6241 gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt ggaagtgaaa
     6301 gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg gattactgat
     6361 ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa tatcgaagtc
     6421 attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat ctccctagat
     6481 gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat ctttattaat
     6541 ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca gacgacggga
     6601 atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc aggaatgatc
     6661 gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac aaatacaacg
     6721 aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga tcagataaaa
     6781 gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa tcagttagat
     6841 aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata gtgtatcgtt
     6901 tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa agttggcacg
     6961 ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat gataggggtt
     7021 tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg cacgtataga
     7081 tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag gtgttgaacg
     7141 tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc tactaaaaga
     7201 agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga ttcaaatcta
     7261 tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc cccaagatgt
     7321 tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg gtatgagttt
     7381 tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag atattgattc
     7441 ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac ctgctgacag
     7501 gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag aatggttgtt
     7561 ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt tgaatttgtt
     7621 acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa gtcatggaca
     7681 atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa
     7741 agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat tggaagaagc
     7801 ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat ggggcggaac
     7861 accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg caatcgtcag
     7921 cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa tggaatctgc
     7981 tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga
     8041 agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac ctaaaggagc
     8101 acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag cacgtgttga
     8161 ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc aaccaaaccg
     8221 tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac taatcgaaca
     8281 agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga tcgaagtagc
     8341 aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc ctgaagacgt
     8401 attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt caatggcgca
     8461 ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag acgttgaagc
     8521 gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc
     8581 acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata
     8641 aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat tttagtcgta
     8701 ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt tcatcaacca
     8761 ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg tatcgtactt
     8821 ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc tgtagcacca
     8881 gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg acaaggtgtt
     8941 aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg gttgttctta
     9001 acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc ggctgctgaa
     9061 gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt
     9121 cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt gcaatcattc
     9181 cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg aatggtcgta
     9241 gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg gccattcttc
     9301 gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct aggtgcttta
     9361 ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc atcaaataat
     9421 ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta ttaatcttga
     9481 aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg
     9541 ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg caaaacggtg
     9601 gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa gaagaacgtt
     9661 ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt gcttctccaa
     9721 ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg
     9781 tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt ggtgacccag
     9841 tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta gctatgggag
     9901 gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt tgggcattca
     9961 tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat gacctttctg
    10021 gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc gtattagctg
    10081 cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact gtaaaattag
    10141 ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga atcaagagtg
    10201 catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca acattacaag
    10261 acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg ttatgtatgt
    10321 ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc gtagtcggtg
    10381 ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg cgttgtcccg
    10441 agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa aatggttcaa
    10501 tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg tctgacagat
    10561 tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg agatagacgc
    10621 aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt catgctaaag
    10681 ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata cacattttct
    10741 tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga agatagaatg
    10801 gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc
    10861 aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta ctttgcagaa
    10921 gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat gctaaaattg
    10981 attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt tggagccgtg
    11041 atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat
    11101 atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga atggggacca
    11161 aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt ggtagcagtt
    11221 gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac taaaaggagt
    11281 tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag aagttttccg
    11341 gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa aaaagcgtgc
    11401 aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa aaaaatgcaa
    11461 gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta cttaacgaac
    11521 tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt tattactgat
    11581 tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat taaaaatgtc
    11641 ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca ctcattagct
    11701 tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat catcgatgcg
    11761 gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga tgaagaagaa
    11821 atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca catcctgaaa
    11881 atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt ttatatgcgt
    11941 tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt gcgttcggct
    12001 atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat cacattggac
    12061 tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc tattggtgat
    12121 cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt agcagttatt
    12181 gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag agattatatt
    12241 tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat cggtttagag
    12301 atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc aggaaatgtg
    12361 atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat tgaggatgac
    12421 ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga attgatcatc
    12481 ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga ctttgttata
    12541 gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa gagataataa
    12601 agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag ttttcttacc
    12661 acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta ggcaaaggga
    12721 ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa gatttaggag
    12781 agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct tctaaagtag
    12841 aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta ttaggacgtg
    12901 cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa gtagatcttt
    12961 actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg caggaacggg
    13021 tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat atccatgtag
    13081 tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt gaagacgaag
    13141 aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc attgcaggct
    13201 ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt caacgcaatc
    13261 gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc tgtttatctt
    13321 gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt gattgaaaaa
    13381 catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt gatcttgcgt
    13441 atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc tttaaatgaa
    13501 gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt caacggtgtt
    13561 ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt tttctatgaa
    13621 tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac acaaaaaata
    13681 tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga aaaaatgcag
    13741 gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc cggattagtc
    13801 gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa agaacgtgta
    13861 gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat ttctgaagac
    13921 gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg tatattggtc
    13981 caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat ccgccctgaa
    14041 aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg taaaccggac
    14101 aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc tatcgatccg
    14161 gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc gatggctcag
    14221 ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga aaatctggct
    14281 gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca ttttgtcaca
    14341 aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg tgatgctaac
    14401 ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg ctggatcact
    14461 cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca aaccgtaaaa
    14521 tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac tgtcactaca
    14581 ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg cgtatattta
    14641 acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt cagcttaaaa
    14701 gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa attacgattc
    14761 cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata tgaagcggga
    14821 ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc tttatatcaa
    14881 gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg gcctaaaaaa
    14941 atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg agcggttatc
    15001 cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt tttgttccca
    15061 acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca gagagtagaa
    15121 gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat tgaggattta
    15181 tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac gccggtgatt
    15241 tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga tgttcgggct
    15301 gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga gttgatgcgc
    15361 attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca gcgcaaacaa
    15421 gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag attgtatgaa
    15481 ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac acagcaaatt
    15541 tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca agtggctccg
    15601 ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg cttagaagag
    15661 aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt tcaatctctt
    15721 gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac gttaaatgat
    15781 caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat ccattatcaa
    15841 gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa atatggaaaa
    15901 tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt tagaacaagg
    15961 agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat taagcaaaca
    16021 ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga cagaatcaaa
    16081 tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact gacttttcag
    16141 ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag catacaacgg
    16201 atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa agaatacgtc
    16261 ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt cccgtctatc
    16321 aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat gatttgcctg
    16381 cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta ttcggcgaag
    16441 cattagctat attggccgga gatggtttgc ttacaggagc attccagcta atcagtatgg
    16501 cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt tgtgcgggat
    16561 cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa aagctttctt
    16621 tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt tatgccttgc
    16681 tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt caacgattag
    16741 ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg attggtacaa
    16801 cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat acttacccgc
    16861 gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta tccgccaaca
    16921 agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg atgcgccaga
    16981 tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac gcgtagatgt
    17041 attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt cagtgatggc
    17101 tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa aaattccagt
    17161 gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag ggggattaaa
    17221 attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat tgttagatat
    17281 tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa aaatgagtta
    17341 tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc ctagagtcgt
    17401 cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct taggaattcc
    17461 agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc ctccattaca
    17521 tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat tcgaagcagg
    17581 cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc aaaaagtttt
    17641 agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat taagttattc
    17701 tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga aagtacccga
    17761 aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg cacacgaaca
    17821 gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg gattcttctt
    17881 tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg agcgaagacg
    17941 taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag caagatatcc
    18001 gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt actcaagcta
    18061 cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca gatggcggct
    18121 accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg gaaaaactat
    18181 taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg aaaacattgc
    18241 cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa gaattatttt
    18301 cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac caggcagttt
    18361 tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga tgctacaaga
    18421 aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc acgaaggaat
    18481 gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg ctttaggtct
    18541 gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa aatgtgtgct
    18601 tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg ttgaattagg
    18661 gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa ctggaaaaaa
    18721 tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa tcggcagtta
    18781 tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg aatcacatct
    18841 tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga gttatcagca
    18901 agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc aaaatgaaaa
    18961 atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag ctgctgctga
    19021 tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta gcaattatca
    19081 aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg aacaaaacag
    19141 tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc tagatgtgga
    19201 gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag atgcaatcgg
    19261 cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag aagaagttac
    19321 gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca ttgagtcaat
    19381 attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt cagaaggtca
    19441 attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc aacaagccgc
    19501 agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca ttctgcatga
    19561 attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa aagaagaaaa
    19621 tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga ccaatccagg
    19681 agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc ggatgttgct
    19741 tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt ttgatgaagt
    19801 ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt ctcaaatatc
    19861 gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag tcgccgacta
    19921 tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg tttcagagtt
    19981 gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg agattacaaa
    20041 actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct gagccacctc
    20101 gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac atgacactca
    20161 ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa atgacaaatt
    20221 ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc aacctcgtca
    20281 ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg cttttttata
    20341 aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat gttaatagaa
    20401 ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc attccataga
    20461 tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa attttcataa
    20521 tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt tctactagtt
    20581 ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca tcacgtaaat
    20641 gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct ttcaatgcat
    20701 ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata tccatgattt
    20761 gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta tcgcattgag
    20821 attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg aattgaaaga
    20881 agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag actttcttta
    20941 aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc aaaataatag
    21001 agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca aaaggatatt
    21061 gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct gtacgggaat
    21121 tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat tgatgtgtca
    21181 gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt tggtcagttt
    21241 cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc atgattttcc
    21301 tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg gaatgtgttt
    21361 gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt gtaaagtata
    21421 tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac attcacacca
    21481 cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca atttcttgta
    21541 aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc gattcctttt
    21601 ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat cttaccgttt
    21661 ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc atattattat
    21721 gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt tctgttgaca
    21781 atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg aaaaaaatag
    21841 aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa aaaatgttta
    21901 tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg ggggattgtg
    21961 gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg gtgaattcca
    22021 acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg aagaacttgg
    22081 cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc cgatgagcga
    22141 atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag acgcacgtac
    22201 ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac aaggacgaat
    22261 caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg tagtcatcgg
    22321 tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt ctgcagcagc
    22381 agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat aagaatagga
    22441 agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa agaaacggta
    22501 gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg cggtgccgga
    22561 catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc gtttgaccaa
    22621 gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga acaaggtatg
    22681 gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga gcatgtagat
    22741 aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga tgaagcagaa
    22801 cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca gtcagctcct
    22861 ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa gattttcttc
    22921 cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa
    22981 acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt catcccagct
    23041 cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt gcggattgca
    23101 gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg gttattgaaa
    23161 gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat tgtcaaaagt
    23221 atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt catacctgac
    23281 gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc agaggaagag
    23341 ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa aataagaatg
    23401 aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat atgatttacc
    23461 tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc aagaaatcat
    23521 tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa aaattgctgt
    23581 ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa
    23641 tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca
    23701 agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac ggattaaaca
    23761 aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag tgaagtaaat
    23821 gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc cgatgaacaa
    23881 tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct ttttatttgc
    23941 cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat tagctgaaaa
    24001 aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa cgatttatga
    24061 tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag cgatactctc
    24121 aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt ttgataaaat
    24181 agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga atattttaga
    24241 ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga aaaatatctc
    24301 ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg ctggtcttta
    24361 ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc attttattgg
    24421 atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt tggagtctgc
    24481 ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag ataattttca
    24541 aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac aagatatcta
    24601 cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc aagtaaatga
    24661 agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg gagaaatctt
    24721 ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga ctggaaaaga
    24781 tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa ccatgaaagt
    24841 tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa ccttccaatc
    24901 tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg aaaaaggtgt
    24961 gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca tactggaaca
    25021 gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac aaagtactca
    25081 ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg tcgaccgagc
    25141 aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga aagctttcac
    25201 ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag tggtggatcc
    25261 ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt tttcaaaaga
    25321 aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa actacggtag
    25381 tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg gaacagccca
    25441 aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt attcgattgt
    25501 ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga aacaccctaa
    25561 gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga
    25621 ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag tatccgtagc
    25681 cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa gtggactgca
    25741 acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca acggtgatca
    25801 gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga tgcctgacgt
    25861 aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta ttgaagttag
    25921 ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa aaatcactga
    25981 agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa aagagcatgg
    26041 aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt gccgcaatgc
    26101 cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt gaggagggac
    26161 caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg tttctgatcg
    26221 gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact cctactcttt
    26281 tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat gattttataa
    26341 aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt ggacagattc
    26401 tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt gtattgaatt
    26461 tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc ttctggcttg
    26521 ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt gctggcttag
    26581 gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag tatgatgtat
    26641 taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac aacagaaaac
    26701 ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt ttagcggcaa
    26761 tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc tatgttatgg
    26821 aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa cgaattttta
    26881 aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg aagattgata
    26941 cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc gtttggtagg
    27001 agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct tgtcttagga
    27061 ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc acttgtcacc
    27121 gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct ttctttaggg
    27181 ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt ttcattgatg
    27241 gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca agaaatgggt
    27301 attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc aattattggg
    27361 attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact actgaatgca
    27421 ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc aagtggtgta
    27481 gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt ccaactcatg
    27541 ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga agcacatatt
    27601 gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca aaaaaacatg
    27661 acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga attagcgcaa
    27721 agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga cggtgtgtat
    27781 gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc
    27841 gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc aaaattgtat
    27901 ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt accacatcgt
    27961 acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa agcaaccaat
    28021 attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt gttattggct
    28081 ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa aggaatcaaa
    28141 gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc
    28201 atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc attagaaaac
    28261 agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga tcaataccca
    28321 aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa atagaaaaag
    28381 tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag ggggacatat
    28441 ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg ctgaattcat
    28501 gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag gaatgccttt
    28561 ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca tcaagacggt
    28621 ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga
    28681 tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg cagccaaaat
    28741 gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca ataaattttt
    28801 aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct tttttcctga
    28861 aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc
    28921 agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga tttttggcgg
    28981 tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg aatatattcc
    29041 ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga tcctaaataa
    29101 aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata tggcagaggt
    29161 catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg cagaattcac
    29221 agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg atcatcagac
    29281 taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag ataatgaact
    29341 aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa
    29401 gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac gtctttatga
    29461 cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa
    29521 ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg gcaaaaggct
    29581 aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa agaagacata
    29641 acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa
    29701 gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac
    29761 ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag tcaacggcac
    29821 aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc gctgattttt
    29881 ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc aggcaataag
    29941 acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga aaatgtttgg
    30001 ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca gccaagaatc
    30061 gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt aacagaatat
    30121 aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga aaatggaaca
    30181 gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga agagttcaaa
    30241 gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc agaattacaa
    30301 aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct gatccaattg
    30361 aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa ccaaatgaaa
    30421 tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat ggaagttggt
    30481 atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca atcggaagaa
    30541 acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg
    30601 caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc aaatgattca
    30661 agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt aaatgaaaat
    30721 tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat ttatggtaga
    30781 attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc tagttgttcg
    30841 cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca ggaatgtatg
    30901 taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat atcgacagcc
    30961 aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga ggcattatcg
    31021 ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca gaagaaaaag
    31081 caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa
    31141 attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat gaagatgtca
    31201 gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa
    31261 tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct cgtggaatga
    31321 tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg atcatccata
    31381 acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg attactcctt
    31441 tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg atcgtcattg
    31501 atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg aagtttacca
    31561 atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta ttaaatacgt
    31621 catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca gaacggacat
    31681 caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg
    31741 aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac aaagcaaaag
    31801 atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg acaggcggag
    31861 ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta aatgtaaaat
    31921 tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta ataagtatcg
    31981 tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct gttacgggtg
    32041 aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca tatgagagct
    32101 atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc ggagaaaatg
    32161 taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc ctgggaggaa
    32221 taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat caaagtcatc
    32281 ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt
    32341 gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa agctgaaaca
    32401 gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca gccagaagtt
    32461 ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga cggtgcagat
    32521 atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc accaatcgtt
    32581 gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg tccattcact
    32641 tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat
    32701 gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga taaaaagaca
    32761 ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt acaaggtatc
    32821 tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt gaaaacagtg
    32881 atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga agatcgcgta
    32941 gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat tgatggagct
    33001 gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga agcacaagat
    33061 gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct aggtacatct
    33121 atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa
    33181 tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca atcacctgct
    33241 caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga agaaaacagt
    33301 tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga tgattccaat
    33361 tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac aaaatctcat
    33421 ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag gagaggaaga
    33481 gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt cgtgtgctct
    33541 tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg ttgaatccgc
    33601 tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc gagtagataa
    33661 attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc atttaattgg
    33721 taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact ttcatgcgtt
    33781 ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat tatcctgttt
    33841 tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag atgagctttt
    33901 accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt tgatgactat
    33961 ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc tgaaagaatt
    34021 gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat taagtatggg
    34081 gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc gtgtaggaac
    34141 agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa
    34201 ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat tatcctgaaa
    34261 caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa tcaactcatt
    34321 ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc
    34381 atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa tcacctacaa
    34441 aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa gcaaatcttg
    34501 ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg attgctaaac
    34561 gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa aaacaagcga
    34621 gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat atcaaacgtg
    34681 ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt tcaaccgctc
    34741 aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga tttatactat
    34801 tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta tcttgatggt
    34861 atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac gattattagc
    34921 taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga ttggaccctt
    34981 ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag gtttagcaca
    35041 gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa atctttattc
    35101 tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact ttcgggcaga
    35161 tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag gacaatatgc
    35221 accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa cactgattcg
    35281 acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag aaagaaaaag
    35341 atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa cagcattgta
    35401 tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc ttggaacttt
    35461 agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg gtgatcgctg
    35521 gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa caaagattgg
    35581 aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc cgaaagatag
    35641 ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga tctctgagat
    35701 ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa
    35761 ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta tttcagtcag
    35821 aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag aaaagtacag
    35881 ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat ggctttaact
    35941 ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa tcaagacgaa
    36001 gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca aagaaatcga
    36061 gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa tgaattaaaa
    36121 gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt aaaaacaagt
    36181 gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga tgagttagtt
    36241 gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa agcaagaaaa
    36301 atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga agatcttaag
    36361 aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca attagaacaa
    36421 gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt gactgacagc
    36481 catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga tactgatgta
    36541 aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca ggctattgat
    36601 atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact ttgttattct
    36661 taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa gatcctctac
    36721 ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt tattggtcac
    36781 agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca tgttcgtccc
    36841 ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg
    36901 aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg taatttgcca
    36961 aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa
    37021 gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata tgccaatggt
    37081 aatatccatt taggacattc attgaataaa atcagtaaag acattatcat tcgttcaaaa
    37141 tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg attgccgatt
    37201 gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg
    37261 gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga tttcaaacgc
    37321 ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc ttatgaagct
    37381 gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa aggattgaaa
    37441 ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat tgaatataaa
    37501 gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa agatattctt
    37561 gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc agcaaaccaa
    37621 ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg ccgtaagttt
    37681 gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga aaatgtaaca
    37741 gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca tccattctat
    37801 gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg tactggatta
    37861 gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa atataaacta
    37921 tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc tggatttgaa
    37981 ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga aaaaggagct
    38041 ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac gaaaaagcct
    38101 gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg tcagaatatt
    38161 ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg tttgtataat
    38221 atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt
    38281 ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat cgaacatgtg
    38341 gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc gaaagaactt
    38401 cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa agaaaaagat
    38461 atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg tcagcgacca
    38521 gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg tggctggttc
    38581 aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa atcagttatt
    38641 tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc
    38701 attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt atgggtatct
    38761 agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca agtggcagaa
    38821 gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga agatttcgat
    38881 cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata tatgaccgtt
    38941 cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata taacttcatg
    39001 catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt ctacctggat
    39061 tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa
    39121 actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat cattccgcat
    39181 acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca gttaagcgag
    39241 tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg gactgctttc
    39301 atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag caaattgatt
    39361 ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg tgaaatgtta
    39421 aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta ctttgaagtt
    39481 atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt tgctattaca
    39541 gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga
    39601 gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga agacttctat
    39661 cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt
    39721 tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac gataccagtt
    39781 atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag ctgctttagg
    39841 tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc aagcttttcg
    39901 aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga agttcgttcc
    39961 atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat tttcaaccat
    40021 ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt gccctacttc
    40081 tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg ttagaacatt
    40141 aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata cattaacagg
    40201 aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg
    40261 tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc ctgcgacgaa
    40321 tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct tgaattgtac
    40381 accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca cttcttcttc
    40441 gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt ctttttcgga
    40501 aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac
    40561 catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta caccgaggtt
    40621 ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag cctcaaaaat
    40681 attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta aataatgatc
    40741 aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca attcaaaagc
    40801 agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc cattttttgt
    40861 cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag gtgccatagc
    40921 caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt cagacaattc
    40981 ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt aaaaatggct
    41041 tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt ctttgacaac
    41101 ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag caaaatgctc
    41161 gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac gctgggctag
    41221 gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca tgtggataca
    41281 ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac ggggattttc
    41341 tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac gatctcatat
    41401 tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt ttcatcaagc
    41461 actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg tcggcgttct
    41521 tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg ttcaggatga
    41581 ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc caatacttca
    41641 gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa aacttcccat
    41701 aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt caattgtaca
    41761 ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat catttcactg
    41821 acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac tttcgttgta
    41881 tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt catattcgat
    41941 catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg ttaattgact
    42001 atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa acgaaagatg
    42061 atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg agggatctat
    42121 ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt ttgttcggga
    42181 taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata ttcatctatt
    42241 tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc
    42301 tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata ctttgagagt
    42361 ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga gagaacggta
    42421 atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct ttatgagaaa
    42481 ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa agctaaaatt
    42541 tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat aggatacata
    42601 gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt tttttattga
    42661 ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa ttattgcgca
    42721 tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag tggctattgt
    42781 atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg
    42841 tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg gtattttaga
    42901 cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc gtgaagtaac
    42961 ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa aagcatttgt
    43021 tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg atgcgcccac
    43081 actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg gttatgccgc
    43141 ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc atgaagaaat
    43201 gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc
    43261 ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc catttgatgt
    43321 agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga tcaacttagg
    43381 tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa gcacaaaaaa
    43441 atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat ttggttctca
    43501 ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata ttactcatat
    43561 ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta agcaagcaat
    43621 tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa aagaaatgga
    43681 tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag caagaaatat
    43741 ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg agataatcgg
    43801 tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag atggagaaga
    43861 accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag gtgtttacct
    43921 tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt tcccaagaaa
    43981 gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga attttgatgt
    44041 atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt attttgactc
    44101 aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc
    44161 gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg cagatgaagc
    44221 agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat tgttgtatga
    44281 agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg
    44341 cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt tgtacaagta
    44401 tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc ttctcttatt
    44461 caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt ttctcctaca
    44521 ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga accaggtgaa
    44581 gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt agcagatgtt
    44641 gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg cggagccaac
    44701 gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg tacaccagga
    44761 agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta
    44821 tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga tcaaattctt
    44881 catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga tcgttccctt
    44941 gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac agcagaggac
    45001 gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa gaaagatgac
    45061 tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt taatcaactg
    45121 actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt aggaagtctg
    45181 gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa aaagaaaatg
    45241 gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac aggtcttcct
    45301 tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg ttcgggaaga
    45361 actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa taatctaaga
    45421 gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt tttatacgga
    45481 ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag
    45541 tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga aaaaataaga
    45601 aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg caaataagcg
    45661 cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg aggggttatg
    45721 tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa ggattgacag
    45781 caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga tatttaagtg
    45841 aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt tactctttgc
    45901 caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg
    45961 aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca aaagctgcga
    46021 ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt acagggaagt
    46081 cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt tctgcagatg
    46141 cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg ctctttggac
    46201 acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt cccggtctaa
    46261 tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta ccgccggaag
    46321 ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt ggagaaagtg
    46381 cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct tcagaaagtt
    46441 tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat cttgcttcac
    46501 gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa gcgaatcgtt
    46561 tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg
    46621 aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa tcttttttac
    46681 attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt tcccttcaag
    46741 tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga ttcatggaca
    46801 gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg tcacaagatc
    46861 cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct ctttccgaaa
    46921 ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat gcttatttcc
    46981 agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg ccagatgaaa
    47041 tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac cgtatttata
    47101 atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat cgtgtgagag
    47161 aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt aagagggaat
    47221 tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt gagattcctt
    47281 ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa agtgagcctg
    47341 ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact gcttcaagca
    47401 tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt aatatgccac
    47461 tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa aactatcaag
    47521 agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac accttcagtg
    47581 atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca agtaccatga
    47641 ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat atttatcaaa
    47701 atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa
    47761 ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga gtagctgcca
    47821 aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg atccagatgc
    47881 agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa gaatatgaga
    47941 tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt ttttcagcct
    48001 atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat gatgagatcc
    48061 cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt tctattgatg
    48121 aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg tcgattatcg
    48181 tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta gacggattga
    48241 tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa atttatcgtt
    48301 tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat caggtaaaaa
    48361 ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa ataaaaaaat
    48421 gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat aaaaaagagc
    48481 taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc ttgcgttata
    48541 taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat catgttagtt
    48601 tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa
    48661 aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa taatttagaa
    48721 tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa agctcaattt
    48781 gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca agactatggc
    48841 atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata aatcaagtat
    48901 gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc atggggttaa
    48961 tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct gctaaaatcg
    49021 gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt atacaagcgc
    49081 atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg caagtgacat
    49141 tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact gatttagcaa
    49201 aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt cttttttgaa
    49261 cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa ctctctttat
    49321 ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga tggagaaata
    49381 catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc
    49441 ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga tcaatgcaat
    49501 tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag cagcagacaa
    49561 aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct ggaacggtac
    49621 actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg cccgagctta
    49681 tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc aaggtatttc
    49741 ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc
    49801 aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag ttggattatg
    49861 gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta tgatcatggg
    49921 agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc
    49981 tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag cgacgatcgc
    50041 tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg ggcaactgat
    50101 cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc aagggttggg
    50161 agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta ttcatggatc
    50221 aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa tcaatattga
    50281 tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag atgcaatcga
    50341 agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg
    50401 atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac gtgcagatta
    50461 tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg aaccagtgat
    50521 gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga ttgctccagt
    50581 agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc cggtatcgca
    50641 gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag gagcggattg
    50701 gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt gggcaccatt
    50761 tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag atgaacgtga
    50821 ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga actgaaatga
    50881 ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa ggatacaatg
    50941 aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc tttgaaacaa
    51001 gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa gcacagcctt
    51061 ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa aaagatagga
    51121 tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg atcgtgttgt
    51181 atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca ggtctttttc
    51241 taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga
    51301 aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt tttacgataa
    51361 agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag taactagaaa
    51421 acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg ctcgcataaa
    51481 aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa cgaagaatga
    51541 attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg ttcattacac
    51601 agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt taggaacaat
    51661 cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta
    51721 ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc tagaaggaat
    51781 ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt aggaaggggc
    51841 agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga aatgtttgat
    51901 ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg ttttcgtaca
    51961 cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct aacaagtcaa
    52021 gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat ggccggcatt
    52081 ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca gatcatggaa
    52141 aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc gccattctcc
    52201 tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag ttatcaacta
    52261 aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg tatggatttt
    52321 gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa ccaacgagct
    52381 ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg caattaccag
    52441 tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta tcagcttgca
    52501 acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt ttgcagcctt
    52561 gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc atgcggctac
    52621 ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat gatcacacct
    52681 tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc atttaaaaag
    52741 agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg gaatgaagga
    52801 atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga acaaacaaaa
    52861 ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg gtatcgtaaa
    52921 gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga agaaacgtta
    52981 agctcaatct tgccacagca acagccaatt cttaatgact atttttgata aaaaacgggt
    53041 gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa ggaaaatgtt
    53101 aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt ttagggtttt
    53161 cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat
    53221 ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta
    53281 cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct
    53341 aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa
    53401 ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat
    53461 aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc tttgcggtac
    53521 aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt tttatataaa
    53581 ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg aacaatatga
    53641 agataagtag acat
//
LOCUS       DADVXN010000007        51257 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.007, whole genome shotgun sequence.
ACCESSION   DADVXN010000007 DADVXN010000000
VERSION     DADVXN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.007"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(56..1561)
                     /locus_tag="KB942_000357"
     CDS             complement(56..1561)
                     /locus_tag="KB942_000357"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBB6850811.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="KB942_000358"
     CDS             complement(1653..2243)
                     /locus_tag="KB942_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6850812.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="KB942_000359"
     CDS             2422..4068
                     /locus_tag="KB942_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6850813.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="KB942_000360"
     CDS             4117..4836
                     /locus_tag="KB942_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6850814.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="KB942_000361"
     CDS             5315..6262
                     /locus_tag="KB942_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6850815.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="KB942_000362"
     CDS             6274..7194
                     /locus_tag="KB942_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6850816.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="KB942_000363"
     CDS             7235..8692
                     /locus_tag="KB942_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6850817.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="KB942_000364"
     CDS             9037..9651
                     /locus_tag="KB942_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6850818.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="KB942_000365"
     CDS             9644..11368
                     /locus_tag="KB942_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6850819.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="KB942_000366"
     CDS             11365..12816
                     /locus_tag="KB942_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6850820.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="KB942_000367"
     CDS             complement(13289..15442)
                     /locus_tag="KB942_000367"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBB6850821.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="KB942_000368"
     CDS             complement(15444..16490)
                     /locus_tag="KB942_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6850822.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="KB942_000369"
     CDS             16640..17929
                     /locus_tag="KB942_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBB6850823.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="KB942_000370"
     CDS             17942..20644
                     /locus_tag="KB942_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6850824.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="KB942_000371"
     CDS             20629..21486
                     /locus_tag="KB942_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6850825.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22456)
                     /locus_tag="KB942_000372"
     CDS             complement(21557..22456)
                     /locus_tag="KB942_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6850826.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            22625..24796
                     /locus_tag="KB942_000373"
     CDS             22625..24796
                     /locus_tag="KB942_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6850827.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="KB942_000374"
     CDS             24864..25205
                     /locus_tag="KB942_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6850828.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="KB942_000375"
     CDS             complement(25481..27235)
                     /locus_tag="KB942_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850829.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="KB942_000376"
     CDS             complement(27246..28082)
                     /locus_tag="KB942_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6850830.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="KB942_000377"
     CDS             complement(28084..28767)
                     /locus_tag="KB942_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBB6850831.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="KB942_000378"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="KB942_000378"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6850832.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="KB942_000379"
     CDS             complement(30359..31858)
                     /locus_tag="KB942_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBB6850833.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="KB942_000380"
     CDS             complement(31851..33272)
                     /locus_tag="KB942_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBB6850834.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="KB942_000381"
     CDS             complement(33332..34324)
                     /locus_tag="KB942_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6850835.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="KB942_000382"
     CDS             34556..36754
                     /locus_tag="KB942_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850836.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="KB942_000383"
     CDS             complement(37212..38084)
                     /locus_tag="KB942_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6850837.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38384..39358
                     /locus_tag="KB942_000384"
     CDS             38384..39358
                     /locus_tag="KB942_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6850838.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39380..40072
                     /locus_tag="KB942_000385"
     CDS             39380..40072
                     /locus_tag="KB942_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6850839.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40038..41546
                     /locus_tag="KB942_000386"
     CDS             40038..41546
                     /locus_tag="KB942_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6850840.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41620..42723
                     /locus_tag="KB942_000387"
     CDS             41620..42723
                     /locus_tag="KB942_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6850841.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42726..44063
                     /locus_tag="KB942_000388"
     CDS             42726..44063
                     /locus_tag="KB942_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6850842.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44050..44928
                     /locus_tag="KB942_000389"
     CDS             44050..44928
                     /locus_tag="KB942_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBB6850843.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44925..45848
                     /locus_tag="KB942_000390"
     CDS             44925..45848
                     /locus_tag="KB942_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBB6850844.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45845..46255
                     /locus_tag="KB942_000391"
     CDS             45845..46255
                     /locus_tag="KB942_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBB6850845.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46259..47827
                     /locus_tag="KB942_000392"
     CDS             46259..47827
                     /locus_tag="KB942_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6850846.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            48067..49485
                     /locus_tag="KB942_000393"
     CDS             48067..49485
                     /locus_tag="KB942_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6850847.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            49497..50807
                     /locus_tag="KB942_000394"
     CDS             49497..50807
                     /locus_tag="KB942_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6850848.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        16518 a         9054 c        10466 g        15219 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgctatg
    38281 taagcgtttt tacttacggt aaaaatgatt atatacgtaa ttcagatagt tatctatcat
    38341 gaagtcataa gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa
    38401 acaagaacca tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa
    38461 aataattgca ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct
    38521 ttatttcttg atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta
    38581 tcaaccattt ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt
    38641 gtttacagaa ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa
    38701 tatcttcgtg ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa
    38761 caaacgattg aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt
    38821 aatcattgtt tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt
    38881 ccttgcaaca atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac
    38941 agtatatatc ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc
    39001 agcgataact gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg
    39061 cttacgcaaa acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct
    39121 gattctaaaa atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa
    39181 ttcattgaat cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa
    39241 gaatggacag ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt
    39301 tatggtaatt ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga
    39361 tagaaagaga gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg
    39421 tattttacag ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg
    39481 agtttttact ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg
    39541 ctcaaaagaa acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg
    39601 tgaaaccttg ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct
    39661 ttctttcata cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt
    39721 gctctcagca ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt
    39781 caaaatgaaa atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat
    39841 attaagtatg gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt
    39901 tatcggtggc agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca
    39961 tttggaatgg aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac
    40021 agtgaaggag aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag
    40081 gaattctagc acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag
    40141 atggaaaagc taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca
    40201 cgattgtgaa cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg
    40261 atgcttcaaa agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg
    40321 ttacacttca aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg
    40381 tattctggga tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag
    40441 atagaatcga agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact
    40501 acgctcgtgc tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc
    40561 caactaccat ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg
    40621 gaaatggtgt ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata
    40681 ccagctttaa acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata
    40741 atggcaaatt cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa
    40801 gagatctatt tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc
    40861 agcaagaaca atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga
    40921 atctgaagac gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga
    40981 tctcagatgg tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct
    41041 tgctagcatt tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat
    41101 ttgttgatga tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa
    41161 cgcattatga attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac
    41221 aagaggtgca accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata
    41281 cagaccctga aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa
    41341 tggatccttc tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa
    41401 aaatcgtcat tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca
    41461 aaaaagctgt agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg
    41521 aagcatacaa agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt
    41581 gtcccaaatc ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa
    41641 ttagcaaaaa gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga
    41701 aaatggtctt atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc
    41761 caagaaaaaa tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat
    41821 ggaacgatcc gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag
    41881 cttttctttt tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc
    41941 ctttcccagc agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa
    42001 atttatcctt atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa
    42061 tttatccaga attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc
    42121 agttataaac atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag
    42181 aaggtccaac catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg
    42241 attggctggt atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat
    42301 tgttctttgc tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa
    42361 gaaaacagcg ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg
    42421 gaagcttccg gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg
    42481 ctgaacaaag aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa
    42541 tttgttctgg aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg
    42601 ttaggcggag cagatcagcg agatggttca tatgcttact acatcagtga accaattatt
    42661 tgtaatgatc aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta
    42721 taaatatgta tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa
    42781 tacaacaagc cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag
    42841 gtgaatttct aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag
    42901 gagcggttct gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg
    42961 aaggagtcca tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct
    43021 ctgtcactgg tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa
    43081 atgaaccaga taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc
    43141 gaattactgt gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc
    43201 tttgcagtaa tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata
    43261 cagacggaat tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg
    43321 tgggagatga ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt
    43381 gcgaaaatat cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag
    43441 gaagtgaaat gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa
    43501 ctgatcgagg aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc
    43561 gagtatccaa tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt
    43621 tctgtggtcc gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg
    43681 aacggacacc agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg
    43741 cttctgcagg ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca
    43801 atatcgatgt ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa
    43861 tagaggaaat gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc
    43921 agatgtcgat tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca
    43981 gtattgagaa attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa
    44041 actttgctta tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt
    44101 ggaaaacgat tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac
    44161 ttaaatgaat tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt
    44221 ccgccggtcc tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg
    44281 atccatggtg ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga
    44341 tatgctgttt ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc
    44401 gagttgttca aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat
    44461 gtgatcagct tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca
    44521 acgattggtc tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt
    44581 gtcaaagttt gtattgatct agcggcacag gtggacatcg aaatactttt gctacatcgc
    44641 cgcctagatc atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg
    44701 ttagcaatcc aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat
    44761 acgatgtgct tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa
    44821 tcaatgggta gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca
    44881 caagaaatga gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca
    44941 aactgataat tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag
    45001 gaatcgatta tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag
    45061 gaagatatga tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa
    45121 cagtagttat ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag
    45181 ggactttccg aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc
    45241 acatcgaaaa tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt
    45301 caggagatcg cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca
    45361 cgggtccgtt gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca
    45421 atcctgagta ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca
    45481 tatttggtgg ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa
    45541 atcctggata catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg
    45601 aaagctgtac cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc
    45661 gtccgtatgc aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg
    45721 gttgggataa ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata
    45781 acaattatga aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc
    45841 agaaatgaaa agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat
    45901 tttctttggc gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag
    45961 ttatcagtcg cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt
    46021 acaggacatg actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagt
    46081 agcattgttt ttgactgcta aaggaaaata cttaacaact gccactggtt tgtattttat
    46141 aacaggattc atattgttga ttggcactca gtttattgcg tttccttttg cattttttta
    46201 tttcgcagct ggggcattca gtttatatcg agtaagaatg agaaaaggag cctgaatcat
    46261 gcagactatc aaagtgaaaa atcctatttt aagaggattc aatccagatc ctaatatatt
    46321 aagagtgaaa gagcggtatt acattattgt ctcctctttt gaatggcttc caggaatccg
    46381 agtatatgag tcgaaaaatt tagtcaactg gtcacataca acggatattt tgaccactca
    46441 agtatctttg caaggaaatc ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc
    46501 tgatggattg ttttatcttg tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga
    46561 tgtccacaat tatttgatca ctgcatctga gatcacaggt ccttggtccg aaccaattta
    46621 tttaaatagt aacggctttg atccttcttt gttccatgat cttgaaacgg gaaaaaaatg
    46681 gcttgtcaat gaactatggg attatcgtct ggacacaccg aataaatcca gcggtatcgt
    46741 attacaagaa tatgatgcag agaagaagaa gcttgatggt cccatctata aaatatttgc
    46801 aggtacagaa ttagcgaaaa cggaggcacc acatttgtat tttcgtaacg ggtattatta
    46861 tttgatgacg gcagaaggcg gtacaggaag aggacattca gttacagttt gccgagcaaa
    46921 gaatattact ggtccttacg agcttgatcc tggttttcct atgctgacag ctagtgacaa
    46981 acctgcttct acattgcaat gtacaggaca tgggagctta gtacaaaccc cgtcaggaag
    47041 atggtacatg acatatcttt gtaccagacc attaaacggt gcggctattt tgggaagaga
    47101 aaccgctatt caagaagtgt attggacgtc cgacgattgg ttaagactta aagatagcca
    47161 gacaactccg atggaagaaa taatgattga gacaaatgaa gaggtacaac aagaaaaaga
    47221 tcatcagttt atggatacat ttgaaggaga gctaaagaag aactggaatg ccagacgcat
    47281 tttgccaaat gctgattggt gtgatacaaa atgtagacca ggttttttac gtctgatttc
    47341 cggtgaatct cttcaatcta ccttcgaaca tcatttgtta gctattcggc aaacggattt
    47401 tacttttgat gcggagacag catttgaata catgcctaat aattttaatc agatggctgg
    47461 actggtactg tacttaaatg aatccaatta tatttattgt tatgttactt ggaatgagga
    47521 caagggaaaa tgcctacgta tgatagaatc agaaaacgga gtagcacaaa tagaagattg
    47581 gacgattcca ctcacagaaa agaagacttt tttgaaagtc actgtaaaga aagagacagc
    47641 acagtttcat ttgaaagacg tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa
    47701 catgtgtata ctctcaggag ggtttactgg aaattttatc gggatagcag tgcatgatct
    47761 aaataaaaaa agaggaagtt atgctgattt tgagtttttc aagtatgatg gaaaagatca
    47821 tctgtaattt aattctttaa taaatgaggt tcactaactt attttcacta gttagtgaat
    47881 ttttttgttt gtatatattg ttatgtaaaa aaagttatca atcttatgca tcaatgtttt
    47941 tagattattt tttgtttttt atctgtcaag ataatgcaat aaaaagccaa cattgtgaat
    48001 ataaaaacaa gaaaaaaaga gttataattt tgaaaacgtt tactagaaga ggtaggagga
    48061 attcaaatgc tttcacaaaa tgctgttaca gcaaatacaa taaaaaaaga aaaaaaggaa
    48121 tttgggattt ccgataagat aggctacatg ttcggtgatc taggtaactg ttttatttta
    48181 ggtttagtca atagtttctt gatgatttat tatacgaatg ttcttggcgt ttcaggggca
    48241 atcgtaggta cgttattctt gatttcgaga attttggatg cgtttgctga tgtgatggtt
    48301 ggaagattat gtgatgtctc agagttaaca gaaactggcc gatttattcc ttggatcaga
    48361 aaaatgaagt atccattttg cttagtgact gtcattttgt ttttgccatt cgtccaacat
    48421 cttccaatga cggtgaagat tgcttatatt tttgtttcat acattgttta tgggattttt
    48481 ctctcaacaa tcaatatccc gtatggagca atggcagcag caattagctc aaatcctaat
    48541 gaccgcacct cgttgtccac gttccgaagc attggttctg cgattggcgg atcaactaca
    48601 ggattcttca ttccgatttt aatgtatacc cataacaaga gtggacaaca agtcgtttca
    48661 gggaatcatt tcttttggat ttcaatcgga tgtgctgcaa tagcatttgt cgcatatatg
    48721 ttaacttgta aattgactac tgaaagagta agagttgtca aaaacgaaaa agtatctgct
    48781 tctcagttgg taaaaggttt gttagctaat cgagcactga ttattttagt cattgttgac
    48841 attttcattg tgatcaatca gattttagct gggacgaata tgacttattt attcaatgat
    48901 tacttccata ataaacaagc aatgtctgtt gctcttttat ttacttacgg aacagtagta
    48961 gttttagcac catttgctac aaagctgaca aaattatttg gtaaaaagga agcaagcgta
    49021 tttgcgttac ttgcttcttc atgtatgtac ttgctgatgt atttccttca tataacaaat
    49081 gcatgggtat atctgattct tttatttatc gctactttag gatctggttt attcaatttg
    49141 atggtctggg cattcattac tgacgtgatt gattatcatc aatataagac aggcttaaga
    49201 gaagatggaa cagtatatgg agtaaattct tttgcgagaa aagtaggaca agcatgtgca
    49261 ggagcggtag gcggatttat gctggcactc attggttacc aatcgtctac aacaggtgga
    49321 gccatccaat cagttttagt acaagaaagg atttatacga tggcaaactt gttaccagca
    49381 ttctgcttat ttgtatccgc aatcattttg ctagtaggtt acccactgaa tcgttcaaaa
    49441 actgtattta ttggtgaaga attgaaaaaa atcaatgaaa gatagggaga aacgtattgc
    49501 ggacaaaaaa agggattatc aatgctttga actttaaaac aaacgacact caacaagatg
    49561 atacaataat actgcaaaaa gcaatcgatc aaggtgcgaa agaacactta cctgtattta
    49621 ttccaaaagg catttattta gtcggagccc tttttttaaa agataagagt catcttatct
    49681 ttgaagaagg tgcagtcctc aaggggagaa cagatatcga agcatttcca gaaatcgata
    49741 cacgagtagc cggcgtagaa atgaaatggc cagcagcgat tttgaatgtt ctctcggcga
    49801 aagatattct tatagaagga aaaggaataa tcgacggtca aggagaccat tggtgggagc
    49861 tgtattgggg aaaagatcaa aaaagcggga caagagctga atacgatcaa aagggacttc
    49921 gttggattgc ggattatgct attaaacgtc cacgcgcttg tttactgtat catgcagaac
    49981 atgttgttgt tcgtgatttg atttttcaga aatcaggttt ttggaacttg caaatcactt
    50041 attctaacga tgtattagta gaaaaagtaa tcatccgcaa caatgatgga ccaagtacag
    50101 atgggattga tattgattct tcaacaaatg tgcgtgtcta tgaatgtgat ttggcctgtg
    50161 gtgatgattg tattgctatt aagtctggca gagatggcaa tggtgcaaga gtcaatcgaa
    50221 aatcatcaag aatcgaggtt gctagatgta aaatccgttc aggatacggt gtaactattg
    50281 gtagcgaagt tagtgctgga gttagtgacg tatatattca cgatatcgat ttttttcagt
    50341 cagactgtgg tattcgaatg aaatcttcaa aagaaagagg gggagtgatc gaaaatatca
    50401 gggtagaaaa tctgaatatg attgatgtac aatttccttt ttcttggata atggactggc
    50461 ataatgaata caatagaaaa aatagcaatg accttgctca aatgccagaa agttggcaag
    50521 cagtggctga agaaatacct gaatcaaagc aaatgacgaa agtcaaagac atacacatca
    50581 aaaacgtaca ggcatgcctt tctccaggtt actcacttcc ttcccgagct tttgatttag
    50641 tagcatttcc tgaaaagcct atagaagatg tctgcttttc tcattgccaa ataactgcaa
    50701 aagaattcgg gcgaatcgaa gcagtacaaa acttatgttt caaaagctgt gatttatcta
    50761 tagaaactgg caacacagct gcaaacaata cttttgacaa tcgttgaaat tataataagg
    50821 tcaagcttaa ataatatttt agcttgacct ttattttttt taatgatata ttaaacaacg
    50881 ttgtcgcgct tcattgaaac attttatgaa aattccaaaa aagagttgac acatgttttt
    50941 agacgtgata agataactaa gtcgttaaaa agaaccactt gcgaagcttg attctatcga
    51001 aaaaaacttc aaaaaagttc ttgacaaata acaaacgata agttatgata aatgagttgc
    51061 tgaaagattt cgaaaaacga aatcgaaaaa aacttttaaa aaagagttga caatcgtttg
    51121 tctctctgat atgatataaa agttgctacg gcagccaaaa atgaatcggg aaacactctc
    51181 gatgaagtag acctttgaaa actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt
    51241 gattcaattc aagacaa
//
LOCUS       DADVXN010000008        50890 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.008, whole genome shotgun sequence.
ACCESSION   DADVXN010000008 DADVXN010000000
VERSION     DADVXN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.008"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            133..504
                     /locus_tag="KB942_000395"
     CDS             133..504
                     /locus_tag="KB942_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6850849.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(778..1470)
                     /locus_tag="KB942_000396"
     CDS             complement(778..1470)
                     /locus_tag="KB942_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6850850.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1473..2258)
                     /locus_tag="KB942_000397"
     CDS             complement(1473..2258)
                     /locus_tag="KB942_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6850851.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2495..3895
                     /gene="lacG"
                     /locus_tag="KB942_000398"
     CDS             2495..3895
                     /gene="lacG"
                     /locus_tag="KB942_000398"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBB6850852.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3920..4237
                     /locus_tag="KB942_000399"
     CDS             3920..4237
                     /locus_tag="KB942_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6850853.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4250..5923
                     /locus_tag="KB942_000400"
     CDS             4250..5923
                     /locus_tag="KB942_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBB6850854.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5937..7568
                     /locus_tag="KB942_000401"
     CDS             5937..7568
                     /locus_tag="KB942_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBB6850855.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8059..9624)
                     /gene="guaA"
                     /locus_tag="KB942_000402"
     CDS             complement(8059..9624)
                     /gene="guaA"
                     /locus_tag="KB942_000402"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6850856.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9683..10606)
                     /locus_tag="KB942_000403"
     CDS             complement(9683..10606)
                     /locus_tag="KB942_000403"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBB6850857.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10802..11407
                     /locus_tag="KB942_000404"
     CDS             10802..11407
                     /locus_tag="KB942_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6850858.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11715..12731
                     /locus_tag="KB942_000405"
     CDS             11715..12731
                     /locus_tag="KB942_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6850859.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12872..14173
                     /locus_tag="KB942_000406"
     CDS             12872..14173
                     /locus_tag="KB942_000406"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBB6850860.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14219..14881
                     /gene="deoC"
                     /locus_tag="KB942_000407"
     CDS             14219..14881
                     /gene="deoC"
                     /locus_tag="KB942_000407"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBB6850861.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14902..15300
                     /locus_tag="KB942_000408"
     CDS             14902..15300
                     /locus_tag="KB942_000408"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBB6850862.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15366..16448
                     /locus_tag="KB942_000409"
     CDS             15366..16448
                     /locus_tag="KB942_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6850863.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16660..18222
                     /locus_tag="KB942_000410"
     CDS             16660..18222
                     /locus_tag="KB942_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6850864.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18215..19348
                     /locus_tag="KB942_000411"
     CDS             18215..19348
                     /locus_tag="KB942_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850865.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19348..20304
                     /locus_tag="KB942_000412"
     CDS             19348..20304
                     /locus_tag="KB942_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6850866.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20403..21569
                     /gene="deoB"
                     /locus_tag="KB942_000413"
     CDS             20403..21569
                     /gene="deoB"
                     /locus_tag="KB942_000413"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBB6850867.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21593..22411
                     /locus_tag="KB942_000414"
     CDS             21593..22411
                     /locus_tag="KB942_000414"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6850868.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22428..23135
                     /gene="deoD"
                     /locus_tag="KB942_000415"
     CDS             22428..23135
                     /gene="deoD"
                     /locus_tag="KB942_000415"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6850869.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23433..24119)
                     /gene="gpmA"
                     /locus_tag="KB942_000416"
     CDS             complement(23433..24119)
                     /gene="gpmA"
                     /locus_tag="KB942_000416"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6850870.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24291..24872)
                     /locus_tag="KB942_000417"
     CDS             complement(24291..24872)
                     /locus_tag="KB942_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6850871.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25005..25688)
                     /gene="rpiA"
                     /locus_tag="KB942_000418"
     CDS             complement(25005..25688)
                     /gene="rpiA"
                     /locus_tag="KB942_000418"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBB6850872.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26007..26420
                     /gene="rpsL"
                     /locus_tag="KB942_000419"
     CDS             26007..26420
                     /gene="rpsL"
                     /locus_tag="KB942_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBB6850873.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26504..26974
                     /gene="rpsG"
                     /locus_tag="KB942_000420"
     CDS             26504..26974
                     /gene="rpsG"
                     /locus_tag="KB942_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBB6850874.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27068..29155
                     /gene="fusA"
                     /locus_tag="KB942_000421"
     CDS             27068..29155
                     /gene="fusA"
                     /locus_tag="KB942_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBB6850875.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29327..30514
                     /gene="tuf"
                     /locus_tag="KB942_000422"
     CDS             29327..30514
                     /gene="tuf"
                     /locus_tag="KB942_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6850876.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30921..31229
                     /gene="rpsJ"
                     /locus_tag="KB942_000423"
     CDS             30921..31229
                     /gene="rpsJ"
                     /locus_tag="KB942_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBB6850877.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31260..31889
                     /gene="rplC"
                     /locus_tag="KB942_000424"
     CDS             31260..31889
                     /gene="rplC"
                     /locus_tag="KB942_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBB6850878.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31917..32540
                     /gene="rplD"
                     /locus_tag="KB942_000425"
     CDS             31917..32540
                     /gene="rplD"
                     /locus_tag="KB942_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBB6850879.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32540..32830
                     /gene="rplW"
                     /locus_tag="KB942_000426"
     CDS             32540..32830
                     /gene="rplW"
                     /locus_tag="KB942_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBB6850880.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32869..33702
                     /gene="rplB"
                     /locus_tag="KB942_000427"
     CDS             32869..33702
                     /gene="rplB"
                     /locus_tag="KB942_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBB6850881.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33745..34023
                     /gene="rpsS"
                     /locus_tag="KB942_000428"
     CDS             33745..34023
                     /gene="rpsS"
                     /locus_tag="KB942_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBB6850882.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34046..34393
                     /gene="rplV"
                     /locus_tag="KB942_000429"
     CDS             34046..34393
                     /gene="rplV"
                     /locus_tag="KB942_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBB6850883.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34407..35063
                     /gene="rpsC"
                     /locus_tag="KB942_000430"
     CDS             34407..35063
                     /gene="rpsC"
                     /locus_tag="KB942_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBB6850884.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35066..35500
                     /gene="rplP"
                     /locus_tag="KB942_000431"
     CDS             35066..35500
                     /gene="rplP"
                     /locus_tag="KB942_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBB6850885.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35490..35678
                     /gene="rpmC"
                     /locus_tag="KB942_000432"
     CDS             35490..35678
                     /gene="rpmC"
                     /locus_tag="KB942_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBB6850886.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35703..35969
                     /gene="rpsQ"
                     /locus_tag="KB942_000433"
     CDS             35703..35969
                     /gene="rpsQ"
                     /locus_tag="KB942_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBB6850887.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36027..36395
                     /gene="rplN"
                     /locus_tag="KB942_000434"
     CDS             36027..36395
                     /gene="rplN"
                     /locus_tag="KB942_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBB6850888.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36432..36740
                     /gene="rplX"
                     /locus_tag="KB942_000435"
     CDS             36432..36740
                     /gene="rplX"
                     /locus_tag="KB942_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBB6850889.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36768..37307
                     /gene="rplE"
                     /locus_tag="KB942_000436"
     CDS             36768..37307
                     /gene="rplE"
                     /locus_tag="KB942_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBB6850890.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37326..37511
                     /locus_tag="KB942_000437"
     CDS             37326..37511
                     /locus_tag="KB942_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBB6850891.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37547..37945
                     /gene="rpsH"
                     /locus_tag="KB942_000438"
     CDS             37547..37945
                     /gene="rpsH"
                     /locus_tag="KB942_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBB6850892.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37977..38513
                     /gene="rplF"
                     /locus_tag="KB942_000439"
     CDS             37977..38513
                     /gene="rplF"
                     /locus_tag="KB942_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBB6850893.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38674..39030
                     /gene="rplR"
                     /locus_tag="KB942_000440"
     CDS             38674..39030
                     /gene="rplR"
                     /locus_tag="KB942_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBB6850894.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39051..39551
                     /gene="rpsE"
                     /locus_tag="KB942_000441"
     CDS             39051..39551
                     /gene="rpsE"
                     /locus_tag="KB942_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBB6850895.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39566..39745
                     /gene="rpmD"
                     /locus_tag="KB942_000442"
     CDS             39566..39745
                     /gene="rpmD"
                     /locus_tag="KB942_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBB6850896.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39789..40229
                     /gene="rplO"
                     /locus_tag="KB942_000443"
     CDS             39789..40229
                     /gene="rplO"
                     /locus_tag="KB942_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBB6850897.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40229..41524
                     /gene="secY"
                     /locus_tag="KB942_000444"
     CDS             40229..41524
                     /gene="secY"
                     /locus_tag="KB942_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBB6850898.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41586..42233
                     /locus_tag="KB942_000445"
     CDS             41586..42233
                     /locus_tag="KB942_000445"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBB6850899.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42424..42642
                     /gene="infA"
                     /locus_tag="KB942_000446"
     CDS             42424..42642
                     /gene="infA"
                     /locus_tag="KB942_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBB6850900.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42675..42791
                     /gene="rpmJ"
                     /locus_tag="KB942_000447"
     CDS             42675..42791
                     /gene="rpmJ"
                     /locus_tag="KB942_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBB6850901.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42809..43174
                     /gene="rpsM"
                     /locus_tag="KB942_000448"
     CDS             42809..43174
                     /gene="rpsM"
                     /locus_tag="KB942_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBB6850902.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43202..43591
                     /gene="rpsK"
                     /locus_tag="KB942_000449"
     CDS             43202..43591
                     /gene="rpsK"
                     /locus_tag="KB942_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBB6850903.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43671..44609
                     /locus_tag="KB942_000450"
     CDS             43671..44609
                     /locus_tag="KB942_000450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBB6850904.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44637..45017
                     /gene="rplQ"
                     /locus_tag="KB942_000451"
     CDS             44637..45017
                     /gene="rplQ"
                     /locus_tag="KB942_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBB6850905.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45289..46128
                     /locus_tag="KB942_000452"
     CDS             45289..46128
                     /locus_tag="KB942_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6850906.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46104..46973
                     /locus_tag="KB942_000453"
     CDS             46104..46973
                     /locus_tag="KB942_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6850907.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46963..47760
                     /locus_tag="KB942_000454"
     CDS             46963..47760
                     /locus_tag="KB942_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6850908.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47963..48709
                     /gene="truA"
                     /locus_tag="KB942_000455"
     CDS             47963..48709
                     /gene="truA"
                     /locus_tag="KB942_000455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6850909.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49374..49611
                     /locus_tag="KB942_000456"
                     /pseudo
     CDS             49374..49611
                     /locus_tag="KB942_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49755..50531)
                     /locus_tag="KB942_000457"
     CDS             complement(49755..50531)
                     /locus_tag="KB942_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6850910.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50654..>50890
                     /locus_tag="KB942_000458"
     CDS             50654..>50890
                     /locus_tag="KB942_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6850911.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKP"
BASE COUNT        16139 a         9267 c        11052 g        14432 t
ORIGIN      
        1 cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt
       61 tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat
      121 gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc
      181 cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca
      241 gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt
      301 gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat
      361 gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg
      421 tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga
      481 gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa
      541 agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat
      601 gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata
      661 cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt
      721 ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta
      781 agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt
      841 tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat
      901 tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc
      961 gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc
     1021 cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca
     1081 gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc
     1141 aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt
     1201 caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc
     1261 tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc
     1321 cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt
     1381 atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt
     1441 tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt
     1501 gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc
     1561 tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct
     1621 cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc
     1681 tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt
     1741 gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg
     1801 acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct
     1861 gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc
     1921 agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc
     1981 tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg
     2041 gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca
     2101 tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata
     2161 tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc
     2221 atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg
     2281 ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg
     2341 gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt
     2401 gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag ttagaaagag
     2461 cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat
     2521 ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg
     2581 cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag
     2641 tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg
     2701 tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc
     2761 agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc
     2821 gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt
     2881 gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa
     2941 agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt
     3001 gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca
     3061 taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg
     3121 tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga
     3181 taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta
     3241 tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca
     3301 ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa
     3361 tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa
     3421 tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat
     3481 cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc
     3541 agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat
     3601 ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga
     3661 tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc
     3721 cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa
     3781 tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc
     3841 aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt
     3901 gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt
     3961 tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg
     4021 aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca
     4081 taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt
     4141 catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca
     4201 agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat
     4261 cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt
     4321 aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt
     4381 cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac
     4441 atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac
     4501 cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa
     4561 caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat
     4621 tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc
     4681 tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat
     4741 gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt
     4801 ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt
     4861 tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg
     4921 aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc
     4981 gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt
     5041 gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg
     5101 aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa
     5161 gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa
     5221 cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt
     5281 gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa
     5341 cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac
     5401 gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat
     5461 ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc
     5521 agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc
     5581 agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg
     5641 cgcagggaca agtgggatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc
     5701 accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga
     5761 tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga
     5821 tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga
     5881 tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga
     5941 aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg
     6001 ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga
     6061 tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg
     6121 ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc
     6181 cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg
     6241 accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg
     6301 atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg
     6361 aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc
     6421 tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc
     6481 cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga
     6541 agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact
     6601 tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag
     6661 gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg
     6721 aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg
     6781 aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag
     6841 ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg
     6901 atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg
     6961 aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca
     7021 gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa
     7081 acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa
     7141 atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc
     7201 aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca
     7261 aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca
     7321 taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta
     7381 atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa
     7441 gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg
     7501 gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga
     7561 aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta
     7621 gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt
     7681 tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc
     7741 taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat
     7801 aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc
     7861 acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta
     7921 tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat
     7981 ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct
     8041 ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc
     8101 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     8161 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     8221 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     8281 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     8341 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     8401 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     8461 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     8521 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     8581 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     8641 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     8701 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     8761 atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     8821 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     8881 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     8941 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     9001 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     9061 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     9121 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     9181 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     9241 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     9301 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     9361 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     9421 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     9481 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     9541 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     9601 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     9661 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     9721 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     9781 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
     9841 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
     9901 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
     9961 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    10021 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    10081 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    10141 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    10201 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    10261 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    10321 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    10381 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    10441 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    10501 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    10561 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    10621 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    10681 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    10741 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    10801 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    10861 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    10921 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    10981 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    11041 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    11101 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    11161 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    11221 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    11281 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    11341 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    11401 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    11461 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    11521 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    11581 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    11641 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    11701 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    11761 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    11821 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    11881 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    11941 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    12001 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    12061 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    12121 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    12181 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    12241 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    12301 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    12361 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    12421 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    12481 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    12541 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    12601 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    12661 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    12721 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    12781 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    12841 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    12901 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    12961 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    13021 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    13081 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    13141 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    13201 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    13261 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    13321 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    13381 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    13441 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    13501 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    13561 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    13621 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    13681 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    13741 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    13801 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    13861 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    13921 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    13981 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    14041 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    14101 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    14161 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    14221 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    14281 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    14341 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    14401 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    14461 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    14521 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    14581 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    14641 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    14701 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    14761 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    14821 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    14881 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    14941 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    15001 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    15061 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    15121 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    15181 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    15241 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    15301 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    15361 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    15421 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    15481 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    15541 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    15601 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    15661 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    15721 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    15781 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    15841 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    15901 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    15961 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    16021 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    16081 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    16141 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    16201 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    16261 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    16321 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    16381 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    16441 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    16501 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    16561 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    16621 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    16681 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    16741 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    16801 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    16861 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    16921 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    16981 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    17041 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    17101 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    17161 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    17221 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    17281 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    17341 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    17401 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    17461 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    17521 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    17581 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    17641 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    17701 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    17761 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    17821 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    17881 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    17941 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    18001 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    18061 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    18121 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    18181 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    18241 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    18301 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    18361 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    18421 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    18481 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    18541 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    18601 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    18661 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    18721 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    18781 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    18841 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    18901 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    18961 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    19021 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    19081 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    19141 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    19201 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    19261 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    19321 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    19381 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    19441 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    19501 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    19561 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    19621 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    19681 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    19741 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    19801 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    19861 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    19921 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    19981 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    20041 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    20101 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    20161 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    20221 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    20281 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    20341 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    20401 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    20461 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    20521 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    20581 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    20641 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    20701 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    20761 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    20821 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    20881 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    20941 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    21001 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    21061 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    21121 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    21181 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    21241 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    21301 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    21361 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    21421 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    21481 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    21541 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    21601 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    21661 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    21721 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    21781 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    21841 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    21901 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    21961 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    22021 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    22081 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    22141 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    22201 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    22261 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    22321 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    22381 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    22441 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    22501 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    22561 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    22621 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    22681 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    22741 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    22801 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    22861 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    22921 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    22981 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    23041 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    23101 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    23161 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    23221 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    23281 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    23341 acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa
    23401 atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt
    23461 aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg
    23521 tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca
    23581 gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc
    23641 aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac
    23701 attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga
    23761 agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt
    23821 tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg
    23881 atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca
    23941 cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt
    24001 ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta
    24061 aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg
    24121 tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta
    24181 caattttttg gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta
    24241 gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat
    24301 aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca
    24361 ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt
    24421 tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct
    24481 tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt
    24541 tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta
    24601 atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat
    24661 ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc
    24721 actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa
    24781 atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt
    24841 atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct
    24901 aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa
    24961 attttccgct gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt
    25021 gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg
    25081 aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc
    25141 tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact
    25201 tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt
    25261 agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg
    25321 actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc
    25381 tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca
    25441 gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct
    25501 aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct
    25561 tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga accggtcccg
    25621 agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt
    25681 aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta
    25741 gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa
    25801 aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc
    25861 tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca
    25921 gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc
    25981 aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc
    26041 gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta
    26101 agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta
    26161 caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa
    26221 acttaattga agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg
    26281 ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc
    26341 gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa
    26401 ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata
    26461 gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc
    26521 tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt
    26581 gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc
    26641 ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat
    26701 gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca
    26761 agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa
    26821 ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga
    26881 tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga
    26941 tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta
    27001 taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac
    27061 aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca
    27121 catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca
    27181 taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga
    27241 acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa
    27301 catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt
    27361 acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac
    27421 agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga
    27481 caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa
    27541 cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct
    27601 tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga
    27661 aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt
    27721 agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc
    27781 tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg
    27841 tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt
    27901 gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt
    27961 agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga
    28021 cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc
    28081 atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca
    28141 tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg
    28201 tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga
    28261 atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga
    28321 ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc
    28381 tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga
    28441 tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca
    28501 agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca
    28561 gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg
    28621 tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc
    28681 agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt
    28741 agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac
    28801 tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt
    28861 aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt
    28921 tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg
    28981 ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct
    29041 tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt
    29101 accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct
    29161 tgaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc
    29221 gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta
    29281 tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa
    29341 atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa
    29401 aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat
    29461 ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac
    29521 agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca
    29581 cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt
    29641 agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca
    29701 agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga
    29761 attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg
    29821 tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata
    29881 cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg
    29941 tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg
    30001 tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt
    30061 tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa
    30121 attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg
    30181 tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa
    30241 attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt
    30301 cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt
    30361 accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat
    30421 ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt
    30481 tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa
    30541 catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt
    30601 tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata
    30661 aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa
    30721 gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt
    30781 gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg
    30841 acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa
    30901 taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg
    30961 aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag
    31021 ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg
    31081 cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta
    31141 tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat
    31201 ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca
    31261 tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg
    31321 aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa
    31381 tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat
    31441 caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc
    31501 gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg
    31561 ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag
    31621 gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc
    31681 gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg
    31741 ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg
    31801 cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta
    31861 ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc
    31921 cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag
    31981 aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg
    32041 cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac
    32101 gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac
    32161 aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc
    32221 ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca
    32281 acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag
    32341 ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact
    32401 ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga
    32461 gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa
    32521 ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga
    32581 aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa
    32641 caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa
    32701 catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa
    32761 acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc
    32821 tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa
    32881 aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc
    32941 acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac
    33001 aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg
    33061 atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat
    33121 ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc
    33181 ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc
    33241 aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt
    33301 gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta
    33361 cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc
    33421 ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac
    33481 atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta
    33541 atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa
    33601 gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct
    33661 aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc
    33721 gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat
    33781 gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa
    33841 acttggtcac gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat
    33901 gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt
    33961 gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc
    34021 taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc
    34081 aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa
    34141 aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat
    34201 tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga
    34261 aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc
    34321 tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt
    34381 atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg
    34441 taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct
    34501 tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat
    34561 caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac
    34621 caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca
    34681 aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg
    34741 caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg
    34801 ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag
    34861 tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg
    34921 ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat
    34981 acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa
    35041 acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga
    35101 attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata
    35161 cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat
    35221 cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa
    35281 atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg
    35341 atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga
    35401 agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt
    35461 aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca
    35521 ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc
    35581 aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg
    35641 cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc
    35701 gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa
    35761 tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac
    35821 gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg
    35881 acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg
    35941 aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg
    36001 caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg
    36061 ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa
    36121 ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg
    36181 tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta
    36241 cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa
    36301 gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca
    36361 tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt
    36421 gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca
    36481 aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag
    36541 gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg
    36601 ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag
    36661 ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg
    36721 gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa
    36781 aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg
    36841 ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat
    36901 caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac
    36961 caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa
    37021 ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt
    37081 ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc
    37141 gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt
    37201 tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag
    37261 aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga
    37321 actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac
    37381 aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc
    37441 tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag
    37501 ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga
    37561 tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt
    37621 agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt
    37681 cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa
    37741 atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt
    37801 gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat
    37861 catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg
    37921 cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga
    37981 gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa
    38041 acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca
    38101 aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga
    38161 aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag
    38221 gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa
    38281 aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa
    38341 ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg
    38401 gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta
    38461 ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta
    38521 gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa
    38581 aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa
    38641 tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa
    38701 acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca
    38761 cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg
    38821 ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca
    38881 gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa
    38941 gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa
    39001 gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc atggtttata
    39061 tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag
    39121 ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg
    39181 gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa
    39241 ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg
    39301 aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg
    39361 gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca
    39421 cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt
    39481 tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat
    39541 taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat
    39601 tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa
    39661 cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca
    39721 cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg
    39781 ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc
    39841 gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac
    39901 aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt
    39961 tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta
    40021 atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg
    40081 aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa
    40141 acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag
    40201 cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt
    40261 aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg
    40321 tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc
    40381 atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc
    40441 aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat
    40501 cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca
    40561 agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg
    40621 attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac
    40681 gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac
    40741 agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt
    40801 gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat
    40861 atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt
    40921 cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc
    40981 tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt
    41041 tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag
    41101 attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc
    41161 tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca
    41221 agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt
    41281 acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt
    41341 tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga
    41401 tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt
    41461 agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga
    41521 gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga
    41581 aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa
    41641 aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga
    41701 tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag
    41761 ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga
    41821 aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa
    41881 tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa
    41941 tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata
    42001 aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct
    42061 atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa
    42121 atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg
    42181 ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt
    42241 gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa
    42301 tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg
    42361 gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg
    42421 tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat
    42481 gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa
    42541 attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca
    42601 tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta
    42661 caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag
    42721 taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac
    42781 gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg
    42841 tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa
    42901 aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga
    42961 acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg
    43021 tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca
    43081 tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg
    43141 cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt
    43201 catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat
    43261 tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa
    43321 tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc
    43381 ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa
    43441 gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt
    43501 acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg
    43561 tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag
    43621 taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt
    43681 tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg
    43741 aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt attttattat
    43801 cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct
    43861 ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag
    43921 cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta
    43981 cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt
    44041 acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac
    44101 gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag
    44161 tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg
    44221 gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac
    44281 cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga
    44341 acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag
    44401 aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt
    44461 tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga
    44521 tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact
    44581 taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga
    44641 gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa
    44701 ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct
    44761 caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag
    44821 ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca
    44881 ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag
    44941 gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg
    45001 tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt
    45061 gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat
    45121 cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg
    45181 acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc
    45241 tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt
    45301 attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa
    45361 gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca
    45421 ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg
    45481 aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga
    45541 atggtttttc aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct
    45601 tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca
    45661 cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg
    45721 caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta
    45781 gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag
    45841 aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca
    45901 gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca
    45961 aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct
    46021 gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca
    46081 gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc
    46141 agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa
    46201 acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt
    46261 tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc
    46321 cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt
    46381 ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa
    46441 actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg
    46501 ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta
    46561 gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa
    46621 cggcaggact tgatccccag ggtagaaaag aaatgatgga gatgttttgg cgtcttcata
    46681 aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg
    46741 cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg
    46801 tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt
    46861 ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg acagctgaag
    46921 aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata
    46981 tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta
    47041 ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt
    47101 ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt
    47161 atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg
    47221 tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt
    47281 gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta
    47341 ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca
    47401 ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt
    47461 tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt
    47521 gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt
    47581 cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga
    47641 ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac
    47701 acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt gagaggataa
    47761 acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcga
    47821 gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt
    47881 ttatctgaaa agaaagaacg aaaaaatttt ataaaaaaat cattattcca gataaccttt
    47941 taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcat acgacggaac
    48001 aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa
    48061 aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga
    48121 tgccggagta catgcgattg ggcaagtaat ccattttgat tatccagagg agcgtccgtt
    48181 agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt
    48241 agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt
    48301 tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc
    48361 ttaccctctt gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga
    48421 tttcacctct ttttgtgcat caggaagctc gatagaggat aaagtacgta cgatctacga
    48481 ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg
    48541 cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg
    48601 attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc
    48661 tacagcacat cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttgtgac
    48721 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattacttct caaaattttg
    48781 caaaatttcg gcttattttc gaaggagcta cttctggaac accgtgaatc agaggttgtg
    48841 aaaaagctgc cctgcatcaa gtaataagaa caaccaaaca aaaatagctc gtcatatttt
    48901 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
    48961 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata
    49021 ttttgccgtt tgttgagcta ataccgaagg tcagctgatt gaacaccgtt taagaaagag
    49081 gtcgtgagcc tgtcatttaa ctctgagtca caagtaacaa tttcaaaaaa cagcttctca
    49141 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    49201 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct taatgaggac
    49261 agtcggaaca ggtttgtcat ttttatattt aaatggcaca aatttttcta attatttgtc
    49321 atttagcgct gaaaattttt gctatttatc taaaaaagtg ataactactt aagatgaagc
    49381 agaatattta cgccatacgg catacaaccg cgaaaaatat aaaaaaaaga aaacgattga
    49441 acgcgttttt gcagtcgcaa aggaaaagca tggaatgcgc tacatcaagt atcgagactt
    49501 ggaaaacaat caggtgtata cgctgattgt ttttgctgca atgaatctga aaaagttgtc
    49561 cacttggcag ttggaacata tgtcttcata tcttttatta tacaaaaata gcaaaagcac
    49621 cagtctacac tcttagtaaa gaggaaactg atccttttgt ctacattctt aaagtactta
    49681 aactaagtac tttattttac tctttaggat tgatacagta gcatctatcc ctttattaat
    49741 aatcttttat ttttttaaat aaagatgttg tctccatctt caggcactaa aactttttcg
    49801 gaaattcctt tttctttatt gaaagacttt aattcttctc ttgacagtcc ccaatggttt
    49861 acagcttcca tatgaacggc aataattgtg gaatctggta ttgctttatg caattcatat
    49921 aaatcgtctt tccccataat aagagattct ccaactaaaa aatgattatc tccagcattt
    49981 gctactatga tttctggatt atactgatca atagtttgtt taacagcttc ataccaaact
    50041 gtatctcctg taaagtatag tgtcttctca gtcgaatgtt tgaaaacaat accgcaaaca
    50101 ttacctacat aattcaagat ttctcctctg ccatgtttgc cttttgtttt aaccaattgt
    50161 atagattcaa aaactgtgtc ttctcgtagt acctccacat ttttaaaacc atatagtcga
    50221 atttcattcg catcattttc atcttgcaca aataatttta tttctttagg taggacctct
    50281 ttcgctatat cgtcccaatg atctagatgt aaatgtgtta caataacagc atcaataccg
    50341 ttgataatat cttcgactgg cattggcaaa tcaactagag gattcatttg atcttttctt
    50401 ggtgatggaa aaggaccttt cgaaataaat ggtggaagtg ttcctttttc cgctaacatt
    50461 ggatcaatta aaaatttttt acctccatag tctaagatag ctgttgcatt acgaatgtga
    50521 tgtatattca taaacgttgt ctcctttttg ttgatatgaa atatttttag tataattcaa
    50581 ttccatacaa taaataaacc gaacaaaaaa agtcttttac tatttttact taatttatga
    50641 aaggttgttt accatgttag accaaacaga tataaatatc attgaggaac tatcaaaaaa
    50701 tagtcgaatc acaatgaaag aacttggaga aaaagtacat ttgactggac ccgctgtctc
    50761 ggcgagagtg acaaaactag aggaaagtgg agttattgaa aattatacag taaaattaaa
    50821 tatggaaaag ctagggtttt cgattcacgc ttttttaatt ataattacgc aaaatttaaa
    50881 tcataaaccc
//
LOCUS       DADVXN010000009        43388 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.009, whole genome shotgun sequence.
ACCESSION   DADVXN010000009 DADVXN010000000
VERSION     DADVXN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.009"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            42..2639
                     /gene="adhE"
                     /locus_tag="KB942_000459"
                     /gene_synonym="adhC"
     CDS             42..2639
                     /gene="adhE"
                     /locus_tag="KB942_000459"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBB6850912.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2868..3974
                     /locus_tag="KB942_000460"
     CDS             2868..3974
                     /locus_tag="KB942_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6850913.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3991..5019
                     /locus_tag="KB942_000461"
     CDS             3991..5019
                     /locus_tag="KB942_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBB6850914.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5025..5504
                     /locus_tag="KB942_000462"
     CDS             5025..5504
                     /locus_tag="KB942_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBB6850915.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5520..6815
                     /locus_tag="KB942_000463"
     CDS             5520..6815
                     /locus_tag="KB942_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBB6850916.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6826..7650
                     /locus_tag="KB942_000464"
     CDS             6826..7650
                     /locus_tag="KB942_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6850917.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7815..8714
                     /locus_tag="KB942_000465"
     CDS             7815..8714
                     /locus_tag="KB942_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850918.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8840..9709)
                     /locus_tag="KB942_000466"
     CDS             complement(8840..9709)
                     /locus_tag="KB942_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBB6850919.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9862..11322
                     /gene="rhaB"
                     /locus_tag="KB942_000467"
     CDS             9862..11322
                     /gene="rhaB"
                     /locus_tag="KB942_000467"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBB6850920.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11319..12575
                     /gene="rhaA"
                     /locus_tag="KB942_000468"
     CDS             11319..12575
                     /gene="rhaA"
                     /locus_tag="KB942_000468"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBB6850921.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12589..13434
                     /gene="rhaD"
                     /locus_tag="KB942_000469"
     CDS             12589..13434
                     /gene="rhaD"
                     /locus_tag="KB942_000469"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBB6850922.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13436..13747
                     /gene="rhaM"
                     /locus_tag="KB942_000470"
     CDS             13436..13747
                     /gene="rhaM"
                     /locus_tag="KB942_000470"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBB6850923.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13829..14980
                     /gene="fucO"
                     /locus_tag="KB942_000471"
     CDS             13829..14980
                     /gene="fucO"
                     /locus_tag="KB942_000471"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBB6850924.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15107..15865
                     /locus_tag="KB942_000472"
     CDS             15107..15865
                     /locus_tag="KB942_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBB6850925.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15965..16609
                     /locus_tag="KB942_000473"
     CDS             15965..16609
                     /locus_tag="KB942_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBB6850926.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16616..17623
                     /locus_tag="KB942_000474"
     CDS             16616..17623
                     /locus_tag="KB942_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBB6850927.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17639..18481
                     /gene="kduI"
                     /locus_tag="KB942_000475"
     CDS             17639..18481
                     /gene="kduI"
                     /locus_tag="KB942_000475"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBB6850928.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18501..19304
                     /locus_tag="KB942_000476"
     CDS             18501..19304
                     /locus_tag="KB942_000476"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBB6850929.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19553..20617)
                     /locus_tag="KB942_000477"
     CDS             complement(19553..20617)
                     /locus_tag="KB942_000477"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBB6850930.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20614..21699)
                     /locus_tag="KB942_000478"
     CDS             complement(20614..21699)
                     /locus_tag="KB942_000478"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBB6850931.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21712..22689)
                     /gene="mvaD"
                     /locus_tag="KB942_000479"
     CDS             complement(21712..22689)
                     /gene="mvaD"
                     /locus_tag="KB942_000479"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBB6850932.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22682..23626)
                     /gene="mvk"
                     /locus_tag="KB942_000480"
     CDS             complement(22682..23626)
                     /gene="mvk"
                     /locus_tag="KB942_000480"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBB6850933.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23942..24601
                     /locus_tag="KB942_000481"
     CDS             23942..24601
                     /locus_tag="KB942_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6850934.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24686..25591
                     /locus_tag="KB942_000482"
     CDS             24686..25591
                     /locus_tag="KB942_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6850935.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25593..26225)
                     /locus_tag="KB942_000483"
     CDS             complement(25593..26225)
                     /locus_tag="KB942_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6850936.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26376..26501
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26545..27069
                     /gene="infC"
                     /locus_tag="KB942_000484"
     CDS             26545..27069
                     /gene="infC"
                     /locus_tag="KB942_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBB6850937.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27141..27341
                     /gene="rpmI"
                     /locus_tag="KB942_000485"
     CDS             27141..27341
                     /gene="rpmI"
                     /locus_tag="KB942_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBB6850938.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27394..27753
                     /gene="rplT"
                     /locus_tag="KB942_000486"
     CDS             27394..27753
                     /gene="rplT"
                     /locus_tag="KB942_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBB6850939.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28005..29342
                     /locus_tag="KB942_000487"
     CDS             28005..29342
                     /locus_tag="KB942_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBB6850940.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29390..31519
                     /locus_tag="KB942_000488"
     CDS             29390..31519
                     /locus_tag="KB942_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBB6850941.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31494..32183
                     /locus_tag="KB942_000489"
     CDS             31494..32183
                     /locus_tag="KB942_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850942.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32591..33268)
                     /locus_tag="KB942_000490"
     CDS             complement(32591..33268)
                     /locus_tag="KB942_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6850943.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33404..33598)
                     /locus_tag="KB942_000491"
     CDS             complement(33404..33598)
                     /locus_tag="KB942_000491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850944.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33648..34088)
                     /locus_tag="KB942_000492"
     CDS             complement(33648..34088)
                     /locus_tag="KB942_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBB6850945.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34092..34937)
                     /locus_tag="KB942_000493"
     CDS             complement(34092..34937)
                     /locus_tag="KB942_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBB6850946.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35086..36504
                     /gene="pepV"
                     /locus_tag="KB942_000494"
     CDS             35086..36504
                     /gene="pepV"
                     /locus_tag="KB942_000494"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBB6850947.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36561..37508)
                     /locus_tag="KB942_000495"
     CDS             complement(36561..37508)
                     /locus_tag="KB942_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6850948.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37731..38081)
                     /locus_tag="KB942_000496"
     CDS             complement(37731..38081)
                     /locus_tag="KB942_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6850949.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38281..39360
                     /gene="pepA"
                     /locus_tag="KB942_000497"
     CDS             38281..39360
                     /gene="pepA"
                     /locus_tag="KB942_000497"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBB6850950.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39504..39824
                     /locus_tag="KB942_000498"
     CDS             39504..39824
                     /locus_tag="KB942_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6850951.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39846..40310
                     /locus_tag="KB942_000499"
     CDS             39846..40310
                     /locus_tag="KB942_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6850952.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40515..41120
                     /locus_tag="KB942_000500"
     CDS             40515..41120
                     /locus_tag="KB942_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBB6850953.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41159..42448)
                     /locus_tag="KB942_000501"
     CDS             complement(41159..42448)
                     /locus_tag="KB942_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBB6850954.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42876..43355
                     /gene="rlmH"
                     /locus_tag="KB942_000502"
     CDS             42876..43355
                     /gene="rlmH"
                     /locus_tag="KB942_000502"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBB6850955.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13701 a         7936 c         9301 g        12450 t
ORIGIN      
        1 aattatttaa caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa
       61 aagaaaaaac gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa
      121 atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg
      181 aaatggcaat ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa
      241 caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt
      301 ggcataatat caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt
      361 tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga
      421 cttccacaac gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg
      481 ctttccatcc aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg
      541 ctatcgctgc cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag
      601 cgacttctat gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg
      661 gaatggtcaa gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg
      721 taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt
      781 cgaaaacatt tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag
      841 aaatctatgc agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag
      901 atgaattgca aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat
      961 caatcgttgg acattctgcg atggaaatcg caaaattggc aggaatcagt gtaccaaaag
     1021 gaacgaaaat gctgattgct gaattggaag gcgtaggccc agattatccg ctatcaagag
     1081 aaaaactttc tccagttctt gccatgatca aagctaacaa cacggaccat gcgtttgatt
     1141 tgtgtgaagg tatgttgaat cttggaggat taggacatac agcagttatc cattcagaaa
     1201 acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt ttagtgaata
     1261 caccatctgc agaaggcggc atcggtgata tctataacga aatgattccg tcattgactt
     1321 tgggatgcgg ttcttatggt aagaactctg tttctcgtaa cgtctctgct gtaaacttaa
     1381 tcaatgtcaa aactgtagcg aaacggagaa ataacatgca atggttcaaa ttaccgccaa
     1441 aaatcttctt tgaaaagaac tctctattgt acttagagaa aatggaaaac gttgaacgtg
     1501 tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta ctgcgataca gttcgtgaag
     1561 tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt gaaccgaacc
     1621 catcgacaaa taccgtatac gcaggaacga aactgatggc agacttcaag ccagatacag
     1681 taatcgctct tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg atgttctatg
     1741 aacacccaga cacatctttc tttggtgcaa aacaaaaatt cttggatatc cgtaaacgga
     1801 catataagat cgacaaacca gaaaaaacac aattcgtatg tatcccgaca acatcaggga
     1861 ctggttctga agtaacgcca tttgcggtca tcactgacag tgaaacacat gtgaaatatc
     1921 cgttagcaga ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa ttcgtcatgt
     1981 cagttccagc atctgtcaca gcagataccg ggatggatgt gttaacacat gcaatcgaat
     2041 cctatgtttc agtcatggct tcagattata cacgtggatt gagcctacaa gcaatcaagt
     2101 tagtctttga ctatttagaa aaatcagtaa aaacacctga tatggaatca cgtgaaaaaa
     2161 tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa cgcattccta ggtatttgtc
     2221 actcagtcgc acataaaatc ggcggggaat acgggatccc tcatggacgg acaaatgcga
     2281 ttcttttacc gcatattatc cgctataatg caaaagatcc acaaaaacat gcgatgttcc
     2341 ctaaatatga ctatttccgt gcagatacag attacgcaga tatcgcaaaa ttcttaggtc
     2401 tgaaaggcga aacaacagag gaattagtcg aagctttagc aacggctgtt tatgaattag
     2461 gaaaatctgt cggcatcaac atgagtctaa aagctcaagg agtcacacaa gaaacactag
     2521 atacaaccgt agaccgtatg gctgaattag cttacgaaga ccaatgtacg acagctaatc
     2581 cgaaagaacc attgatctct gaattgaaac agatcatcat tgatgcttac aacggataag
     2641 caaaagaaag cgtagaaaaa agataagaaa atagatgaaa aagaacttgg aaaacgttgt
     2701 ttctccaagt tcttttttta atttccaagt aactgaaagt tacttttgtt atttaacatt
     2761 taggtcaatt ttagacagtt tttgttcaaa aagcgatatt tatagtggac ggtattactg
     2821 ctatattaag gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg attattaaag
     2881 aagagatttt tgtacctaaa gaattagtga aagaaaaaat tgacctgctc gttgaaaacc
     2941 tgacaacgat caaagacgat aatggtgaat ttttgttgga ctttgatgga ctgaaagtag
     3001 atgacaaaag ctggacagtg tggaattggc cacaaggtgt cggtctatat ggtatttata
     3061 aaaattacca gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa tggtttgagg
     3121 atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg ctattgacta
     3181 tggcttactt gtatgaagac acgaaagatt cacgctattt gccttattta gaacaatggg
     3241 cagaatgggt catgaatgac atgcctcgga caaatgaaga cgggttacag catgccacat
     3301 atggaccgga aaataagaat cagttgtggg acgatacatt gatgatgaca gtgcttcctt
     3361 tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt agaagaagct cgttaccagt
     3421 ttatgatcca tatcaaatac ttgatggata aacgaacagg attgtggtat cacggctgga
     3481 cgtttgaagg aaatcataac tatgccgaag cattttgggc tcgtggaaac tgctggttga
     3541 cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca
     3601 aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa
     3661 gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg
     3721 caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata
     3781 aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag
     3841 ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg
     3901 gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg
     3961 tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca
     4021 agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca
     4081 actgtcgcag atgcagatgt aaatgagata gttaccttgc gctttgctta tgcaagcaac
     4141 agccagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat
     4201 ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg tgaactgatc
     4261 gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc gttggaaagt
     4321 ttctctaaag attattcgat tttttcagtt ccttatctat tcaatgatga agaacatttc
     4381 tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac caaaaattta
     4441 ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat ggtagacggt
     4501 ccgatccata cgccggaaga tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc
     4561 gctatcaaaa tggtagagct tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg
     4621 tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat
     4681 acagctggac atggtggtgt ggctaaatac tattcctatg atgagcatac acgtgtgcca
     4741 gatatcatca tcatgaatga tgccatcaaa gaacagctaa ctgatgagca ggaaaaagcc
     4801 attgaagaag cagcgaaaga atcaacggtt tttgaaattg aagcttttgc aaaagcaatt
     4861 gaagaagaaa aagctttagc acaagaagaa catggagtcc aattcaatga tgtagataat
     4921 acaccgtttc gagaggcagt tgccccactt catgatatat tcaaaaacga tcctacctac
     4981 aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa atgattttaa
     5041 acaaggtatt agagatcctg ggagctgttt tgcttgttgg gatggtcgct gttgttttat
     5101 ggcaagtatt ttccagaacg gttttaggaa atccaaatac ggtcacggaa gagcttgttc
     5161 gattcggtct tgtctggttt gccatgttgt cttccgcgta tgttgtggga caaaaagcac
     5221 atttagccgt aacggtttta agtgaaaaat taactggcaa aaaaagtaaa attttagaag
     5281 ttgttgttca aagtttattt ctggtatttg ctgcgatgat catggtgtat ggcggatgga
     5341 atgctgtcac attgacaatg gggcagatct cgccttcttt aagtattcct atgggatatg
     5401 tttatctgtc agtacctgta tcgggggtac tcatcattat ctatagcctg atcaatctaa
     5461 tcgatacatt aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa
     5521 tggcaataga agccggatta gtgttatttt ttgtatttgt tgtgttgctt gtattaggga
     5581 tgccgattgc tataagtatt gcattctcat cgatgatgac tttattgtta gttataccgt
     5641 ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg aagtatcaat agtttttcgc
     5701 tgatcgctat tccgtttttc gttctctcgg gcattatcat gaataatgga gggatagcta
     5761 agaaactagt ggattttgcg atgttgttcg tcgggcgcgt gccgggggct ttggctcata
     5821 cgaatgtgtt agggaatgct ttatttggtt ccatctctag ctcggccatt gccgcttcta
     5881 ccgcgattgg cggagtgttg atccctcagc aagtggaaga aggatatgac cggaagtttg
     5941 cgacagcggt caatatcgcg tcagctccga caggtatggt cattcctcca agtactgctt
     6001 ttatcatgta ttcacttgtt gctggtggtg cttcgatctc tgctttgttt atgggaggtt
     6061 atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt agcattcatc gttgctaaaa
     6121 agaataaata ccctgttgtc gaaaaaggac agatggctca tgctggaaaa ataataaaag
     6181 aggcgattcc tagccttcta ttgattgtca tcattatcgg tggtatcttg acggggatat
     6241 ttacagcagt agaagcttca gcgatagctg tcatttactc cctgttgatt gctatgtttt
     6301 actataaaac ggtaaaacta aaagatatac ctaatatgtt gtttcaatca gtagcaatgt
     6361 cagggacgat catgttctta ttggctactt cttccatgat gtctttcgcc atggcgttta
     6421 ccggtattcc tcaggcaatt agttcgttga ttatgggcgt aacggataat aaatttatta
     6481 ttctgttatt agtcaatctt gtgttattac tggtgggtat gtttatggat gtggcgcctg
     6541 ctatcttgat ttttacacca atatttttac caatcgtcac aagtgtaggg gttgatcctg
     6601 tacattacgg gttgttttca atcatgaatc tgtgtgtagg ctccatcacg ccgccagtag
     6661 gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca atgctgaaac
     6721 cgcttcttcc tttttatggg gttatcttag tcgtcttgtt tttgattact tacttcccgc
     6781 aaatcgtcat gtggctaccc aatatgctga gttaggagaa tagttatgtt gaacattaca
     6841 attagaggac atgatctttc cgaagtccaa acgatagaag atcttgcaga aaaaacaaaa
     6901 gaacaaggga ttcatacact gcagctcgcc ttaggtcttt cgtttcctga aataccttct
     6961 ggcgcaaatg agatcaattc tgggatggga aattatgtca aacgagtttt agaaaagcaa
     7021 gaagtgaccg taggaatttt aagttgctat atcaatatga ttcatccaga tttaacgata
     7081 cgacaagagt tgctgacaaa gttcgagcag tatcttcgct atgcttcttc ctttggtgcg
     7141 tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa tccaatatac ggaagaaaat
     7201 ttcacagatg aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt aaaagctgga
     7261 gaaaaatacc aaatgatggt ggggatagaa ccaggtttga atcatccggt gtattcttta
     7321 gcaagggtag agcagctgat ccaagcagta gattcggact atctaggaat cattttagat
     7381 ccgaccaatt tgatcacaag tagtgattat caagaacaag tccaattggt agaagaagct
     7441 tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga aagattttcg tgtcgaacag
     7501 gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa aatcaaagaa
     7561 atcatcatga agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa agatgatggg
     7621 attcgttatg gaagaagtct tcttgaataa tgtaaagaag acgttggtta actaatattt
     7681 agaatggagg gggcggggct agaagatgcc acgtctcttc ttttttataa gagtatataa
     7741 aagcagctct ttttgattac aaaaaacgtg gtataataaa attgtgcata agtgaacaaa
     7801 aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga acaagtcaat acgatggttt
     7861 ctgatcagta tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa acgatctatc
     7921 atcatcatga tttctatgag atccatgcta cattaaaggg agaagcgact ttttatttga
     7981 acggacgaca atttacgatc aaagcaggaa gtgtgctgct catccattcc aatgatttgc
     8041 atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt gtcacccctt
     8101 cttttctaga aagtcgttcc acgccatggt ctaatctatc ggcttgtttt caaccaatgg
     8161 gagagaagag gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa aaattatcat
     8221 ttatcgacca tcctcctgat aaaaaaattt acggtgaaga cataaaatat gaacaagctc
     8281 tggtagaata cttgattttt ctcaatcaag ttgtacaaaa agaagagagt gcgcaagaac
     8341 agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt acaatatatc tcaaaaaatt
     8401 tatctcatcc gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc aaataccata
     8461 tcacacgaga atttaaaaag tacactggat ttacgtttca tcagtatgta atgaagaaaa
     8521 agctgatcta tgccaagcac ttgttgcgag aataccgcag ctctagtgct gtatatagta
     8581 aaagtggttt ttcttcttat ccgcattttc tgaaagcatt caagagagag ttcggcataa
     8641 cgcctaaaga atttttgaaa cgtgatacac agaaacagtt ggttcattat gaacattttg
     8701 aagatgaaca atgagaatga tcactaaaaa ataaccgcag tagctgctgt tgtttacata
     8761 gaagcagaaa ctgcggttat tttattttta tctatatatt tcaaaatatt ttgaaaaaat
     8821 gacgtacata ttatatgaat cagctgaatt tacgaaagtc tctcggcgat agtcctgttt
     8881 ttgctttgaa caggcggcta aagtaagcaa aattctcaat ccctatttct tcggaaatca
     8941 ttcgaaccgt aaattctgta taccgaagaa ggtctttagc gtaagaaagt tttttgctga
     9001 tcaaataatc gattggtgga gaaccgataa atttggaaaa atctttgctc agctgatatt
     9061 tattcaataa gaattgttgt tctaattgat caagtgtaat cgtttctttg aaatgttcgt
     9121 ctaagtattg cttcagtttg taaacgtagg cgggaatatc ctcttcttcg aaatctagct
     9181 gatacttttg caaaatcagt tcattcagca actcgtggat agctacagag gactggaaat
     9241 cggtacgggc attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa tggtggatct
     9301 tgttttctga tggatctcct gttgaatgga aggtgttatg gccgtctttt gtgaactcct
     9361 gataaaatga ttggacgttc tgaccattca aatggatcca atccatttcc catggttctt
     9421 cgctgatcgt ttgataacgc tgatattctt ggcagtcaat aaaaaataag tctccagctt
     9481 ttagctgttg ctgattatca ttatatgtaa gcgaaccttt gccagacaaa gtaaacttta
     9541 tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg gattggtgct ttgaagtaac
     9601 cgatttcttg gatataaaaa aataaattcc tgcttctttg tgtaggcgtc aagatcactc
     9661 tttttgagtc ttccgaccat gagtggttgg ctttttccca gtaatccatg taaacactcc
     9721 caacttgtca agataatgca attatatacc aaaatagtgt gaagcaaaat ataagattct
     9781 gtattatgat agcattgtca ataaatgata acgctttatt aataaagtgc taatcaacaa
     9841 taaagtaaaa gaggtggaga aatgaatcca acatttgttg ccatcgatat cggtgcatca
     9901 agcggacgac taatgaaaag tacgagggat gcaaaaggtt ctttgatctt ggaagaagta
     9961 caccggttca aaaacggctt tcggaacaaa gacggtcacg atcgctggaa gatcgattat
    10021 cttatcaaag agattttgat tggatttgaa aaattaaagc agacagggat tgatcgcctt
    10081 tttgcgggga tcgatacatg ggcagtcgat tactgtctga ttgatgaaca aggggatcgg
    10141 ataaatgatc cgattgctta tcgggacaag cgtaccaatg gagcagtaga acagttttct
    10201 gaaatctatt ctctagataa gttgtatgag cagacaggga ttcagatcca gcctttcaat
    10261 accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca taaattgtta
    10321 ctgatacctg attatttagg gtatgtcttt actggtaaca tggtgacgga gaaaactaat
    10381 gcatctacta tgcagctgct gaatgcaggg acaaaagaat gggaaataga acttcttgaa
    10441 aaaatcggtg tctcgaagga attatttcca ccattgacag agccgggaac gatactagga
    10501 gatctgcaaa aagaacaatt ttcaaaatat gatttgccag atgtgacctt tatcacagta
    10561 gcaagtcatg atacggcgtc agctgtatta ggaacgccag gtcaaggaga acgttggggt
    10621 tatatcagca gtgggacatg gtcgctttta gggattgaaa caaagatcgc caatgtttct
    10681 cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg cccataacac ggtgcgtttt
    10741 ttgaaaaaca tcatggggat gtggttgatc caggaagttg cccgaatgca agagtacgag
    10801 tacagttatg cggaattggc agagcttgct gcaaaagaaa aaccgctgca gcaagtgatg
    10861 gaaatcaatg accctcgatt tttgaatccc ccaaatatga tagaagagat tcagcaatac
    10921 tgttttgaaa cagagcagcc agtccctgtc tcatcgggag ctatcgctcg ttgtgtgtat
    10981 gataatcttg ctctttgtta cgcacaagag ctgatcaaag tagaacactt aactggaacg
    11041 aaattggaaa agctgcacat cgtagggggc ggatcgaata atcgtctact gaatcaattg
    11101 acagcagatt atgccggaat cattgtagaa gcaggaccta gtgaagccac agcaatcggg
    11161 aatgttgcca tgcaaatgct cagtacaggt cagttttcga atatagaaca agcaagaaat
    11221 gtaatcaaac agtcgtttcc atgtgaaact ttttacccag atgaaaataa gcaaaacacg
    11281 atggaatggt acgtgaataa aatcaaagga gtaatcgtat gaacacgatt gaaagaaatt
    11341 acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct gtactagaaa
    11401 agatacagga catcaaaatt tctatgcatt gctggcaagg agatgatgtc aaaggtttct
    11461 tgacaccgga tggcgaattg accggtggaa tcatggcgac agggaatttt ccaggagccg
    11521 cacgaacacc tgaagagctt agacaagatc tggagaaggc ttattcgttg ataccgggca
    11581 aacacaaatt gaaccttcat gctatctatt tggatacaga agaatctgtc gatctaaatg
    11641 aaattgagcc gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa gaaatcggtc
    11701 ttgacttcaa tccgactttc ttttcgcatc caatgatgaa agacggcttt acattggctc
    11761 atccagataa agaagttcgt gaatactgga tcgaacacgg gaaaagaagc cgcaaaatcg
    11821 cggagtactt tgggaaagaa actgggcaaa caagtatcaa taacttttgg gtacctgatg
    11881 ggatgaaaga taatccgatt gatcgtttta ccccacggga acgtttaatg gaatcactcg
    11941 atgaaatttt tacagaagag ttagatgaac ggtatgtaca agatgcagta gaaagcaaac
    12001 tatttggttt aggcgctgaa gcttatacag tcggttccca tgaattttat atgggttacg
    12061 ggttgactcg tgacaaactt gtttgtctag atgctggaca ttttcatcca acagaagtga
    12121 tttccaataa attatcttca ttagctttat tcagcaaagg aatcatgttg catgtttccc
    12181 gtccagtgcg ttgggattca gaccatgtgg ttttgatgga tgatgaattg caggaaatcg
    12241 ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg gatttctttg
    12301 atgcaacgat caaccgtatc gctgcttggg tcatcggtac gagaaataca caaaaagcac
    12361 tgctgaaagc tatgctggaa ccagttgagc gattgaaaga aatggagctg gcttttgact
    12421 ttacttcaag aatggcttat acggaagaat tgaaagattt cccgtatgcc gatgtatgga
    12481 attatttctg ttacaaaaat caagttccag ttggtttgga ttggctggaa gaagtccagg
    12541 aatatcaaaa agacgtgttg gaattaagaa aataggagtg tggctatatt gaaaaaaata
    12601 gatattttac aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa tctttatcgc
    12661 ttgggatggg atgaaagaaa tggcgggaat atttcttatt tactaaaaga agaagagatc
    12721 acaccgttct tggatcctga aaaagtgatc cgccgcatct cgatgatttt tgatgccagc
    12781 cgtttagcag gatgctattt tattgtaact ggttcaggaa gatattttaa aaatgtagcg
    12841 agcgaacctg ccgagaatct tggaatcgtt cgagttgctg aagatggcaa aacactggaa
    12901 cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg agttgccaag tcatttcatg
    12961 agtcatatcg cccgtttaga agtagaccca gataatcgga tcgttatgca ttgccatgct
    13021 agtcatctat tagcgatgag ttttacgcat gaattagacg aacgttcctt tagtcgtacg
    13081 ctatggcaaa tgtgtacgga atgcttagtt gtttttccag aaggaatttc catcattccg
    13141 tggatggttc cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat gaaagaaact
    13201 cgattggttt tatggccaca acatggaatc tacgggtcag gaaaagacat ggatgaagtc
    13261 tttggtttga tcgagacagc agaaaaggca gcagaagtct atacgtacgt caaagcgcaa
    13321 ggaccaatct tgcaaacaat cacggacgaa aacctgtggc atttagcaga tgcgtttggt
    13381 gtcactccaa aagcaggtta tttggaggaa gttcatacga aagcaggtgt ctgacatggt
    13441 gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa gaagaataca aaaaaagaca
    13501 tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa cacggggtca tcgactattc
    13561 gatctatctt gatccgagta caagtttgtt atttggttac ttagtgatcg aagatgaaaa
    13621 gttatgggaa aaaatggcaa atacatctat caatcaaaaa tggtggaagt acatggaaga
    13681 tatcatggag acaaatccag atgcctctcc agtcagttgg gaattgatgc cagtctttga
    13741 actgtagcaa caagcaactg ggcaaccggt tgcttggggt tctttcgttt ttattgtgaa
    13801 aaataataca aattaggagg aatagaaaat gagtaatcga atgattttaa atgaaacatc
    13861 gtattttgga aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc gaggattcgg
    13921 caaagcagct gtcattactg acaaaggatt gatcgagcac ggtatagcaa caaaagtaac
    13981 agaacttcta gataaagcag caatcgctta tgctttatac gacgggatcg tacctaatcc
    14041 aacgatccaa aatgtaaaag atggagtggc ttttgttaaa gaggcagagg cagactgttt
    14101 gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa gccattggga ttattttgac
    14161 gaatccagag ttttcagacg tcattagttt agaaggtgtt gctgatacga aaaatccatg
    14221 tttacctata ttagctatac caacgacttc tggaacagca gcagaagtaa cgatcaacta
    14281 cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc atgatatccc
    14341 aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat tggctgcatc
    14401 aacaggtatg gatgctatga cgcatgctat tgaagggttt atcacaaaag gcgcttggga
    14461 aatgactgat atgctgcatt taaaagcaat cgaaatcatc ggccattctt tggaagcttc
    14521 tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta ggacagtata ttgcgggaat
    14581 gggcttctca aatgtcggtt taggactggt acacgggatg gctcacccgt taagtgcatg
    14641 gtacaacatt cctcatggcg tagcgtgtgc agcacttttg ccaactgtca tgaaatataa
    14701 caaagaatat actggagaaa agtaccgtga gatcgctctt gttctaggga ttaaaggagc
    14761 agctgaaatg tctttagaag atgtaagaga agcggcttgt ggtgagatcg atcggttgag
    14821 caaagcggtt ggaatcccag aaaccatttc tgaattaggt gtaaaagaag cagatattcc
    14881 agcaattgca gaagatgctt tgcgagatgt atgcacacca ggaaatccaa gagaaacgac
    14941 agtagaagaa atcattgctt tatatcaaag tttgatgtaa aaacgaagcg gatcttcaac
    15001 attaaattgc ctgtaaagag aaatggtagt ttatatctgg atatctcttt taaattgatg
    15061 gtatttccgc tataattttt cttataaaga aataggagaa tattatatgg aaaataaaaa
    15121 gccttatgga actgtattga tcaaagcctc taaaatacta gactatttag cggaaaacca
    15181 agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg acctcttcta cagtcttgaa
    15241 aatactagat actttgttga tgatcggcta cgtaaataaa aattcagaga aaaactatcg
    15301 tttaggtgca aagcttattc gctacgcgaa taaaaacatt gaacaaatcg atttggtaga
    15361 gttgacttta ccgtttttag aacagctgca gcaaaagatc gatgagacta tccacttagg
    15421 ggtattggac aacaatgaga tcttgtacgt gaacaaacta gatccaaaac atcaaacgat
    15481 ccgcatgtct tcaaaaatcg gaatcacccg accattatac aattctgcga tgggcaaagc
    15541 cgtattagca gaattcagtg aagagcagta cactgattat cttgaaaagc atccgttgat
    15601 cccttacaca gaatatacga tcaccaatgc attacggttg aaaaaggaaa tcaaaacggt
    15661 aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa cgtgatattt tttgtatagg
    15721 agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt agtgtaagta tgccgaaata
    15781 tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc ctcaagcaaa caaaaagcga
    15841 aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg caatttttct tttctgccgt
    15901 ataatatggg tatagatttc caagttataa aataaaattt ctaaatagga aataggtgga
    15961 gaagatgaaa aaaattgata ttttatctcg attgaaaaac gccggagtga ttgcggtggt
    16021 acgcggaaaa agcaaagaag aagctttgaa tgcttgccac gcaatcatca aaggtggtct
    16081 aactggtatt gagttgactt ttacagtccc tcaagcagat caagtaatca aagaactttt
    16141 gtcattctac aaagaccaac cagaaatagt gattggcgca ggcacagtat tagacgctgt
    16201 cacagctcgc ttggctatct tagctggcgc agagtatatt gttagccctt cctttgatca
    16261 agaaacggca gagatgtgca atctgtatca aattccttac ttaccaggat gtatgactat
    16321 taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc gtcaaactat ttcctggaag
    16381 cgcttatgga ccaagtatca tttctgcctt caaggcgccg atgcctcaag tcaatatcat
    16441 gccaactggc ggtgtgagtc tggacaatat gaaagagtgg ttcgatgctg gtgtcgtaac
    16501 agtcggagta ggtgggaacc ttttagcacc cgcagctaca ggagattttg acaaagtaac
    16561 ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc aagaggtagc tgcttatggg
    16621 aaaagtagtg acactcggag aaatcatgtt gcgattgtcc acagagcagg gaacccgtat
    16681 cgattcaagt gaacgattcg ctgcccatta tggcggaggg gaagcaaacg tagcaatttc
    16741 tttagctaat tacggacatg aagcgatttt tgctacaaaa gtacctgata acgcattggg
    16801 aaaagcagtc aaaaagcact tgcaatgtta tggagtagat acgaaacagg tattatctgg
    16861 tggatcaaga ctaggtactt attatatgga aactggtagt ggcgaacgcg ccgcctcagt
    16921 gatctatgat cgtgccggat caagttttgc tgaaatggat catttggaat ggtcgctaga
    16981 ggagttattc caaggaatag atatatttca tgtttcagga atcaccccag ctttgtctaa
    17041 aaaatggcaa aaaatgacga aacaggtgat agaagctgca aaaaaagctg gctgcctgat
    17101 cagcttcgat atcaactatc gtggaaaatt atggacacag caagaagcca gcgaagtaat
    17161 ccatcagctt ttacctttag tagatatctg ctcagctgga aaaatggatg ctctttattt
    17221 gttaggtatc gagaaagcgc cagaagcaga agaacaacct ctaatttatt attatcaaaa
    17281 aatgcaggaa aagttcccga atattcaggt gttttattcg accaagcgta ccgttcattc
    17341 agcaagtgaa aatgaattga aggggacatt gtggatgaac catgaatatg tggaatccca
    17401 agtccataga atcaccccaa tcgtggatcg agtaggtggc ggagatgctt ttgcaggtgg
    17461 cgtgctccat gggattttat ccaatatgtc tccgcaagaa atcattgatt ttgctacggc
    17521 agcttctgcc ttgaagcata cgatccatgg ggactgcaac caatttagtc aagaagaagt
    17581 cgcacaattt ttagcatgcg gctctggaaa aatcattcga tagaaggagt agaagaaaat
    17641 gcaagaaatg gaaacacgtt atacacacag tccagaagat atccgtcatt attcaacgga
    17701 acaattgaga aaagaatttt tagtagaaaa ggtatttgaa ccagggaaaa tcagtttgac
    17761 ttatacgcac aacgatcgaa tgatctttgg tggagtgaca cctactgaaa aagaattaga
    17821 aatcacatta gacaaagaac taggtgttga ttacttctta gagcgtcgtg aactaggtgt
    17881 gatcaatatc ggtggaccag gattcatcga gatcgatggg caaaaagaag aaatgaaaaa
    17941 acaagacgga tactatatcg gaaaagaaac acgacatgtt gtattttctt ctcaagatgc
    18001 tgaaaaccca gctaaattct atattagttc tgttccagct catcataaat atccaaatgt
    18061 aaaaatcagt atcgaccaag taaaaccaat ggagactggt gaaggcctga cactgaatga
    18121 acgtaaaatc taccaataca tccatccaaa tgtatgcgaa agctgtcaat tacaaatggg
    18181 ttatacgatt ttagaaccag gaagcgcttg gaatacgatg ccttgtcata cacatgaacg
    18241 tcgtatggaa gcttatgtct actttgatta tgccaatgaa gatacacgtg tgttccacat
    18301 gatgggcaaa ccagatgaaa caaaacattt agttgtagac aatgaacaag ccattatttc
    18361 gccaagctgg tcgatccatt caggtgtagg tacaagcaat tactcattca tctgggcaat
    18421 gtgcggagaa aatatcacct atacagacat ggatatggtg caaatggatc aattaaaata
    18481 agcataaggg agagaaaatg atggaattta gtatggatat gtttcgttta gatggaaaag
    18541 tagcattagt caccggtgct gtctatggta ttggatttga gattgcacgc tctttagcta
    18601 cagcaggagc aaaaatcgta tttaataact tgaatcaaga atcagttgat gaaggtatag
    18661 cgcattataa agaagcaggc atcgatgcaa aaggttatgt ctgcgatgtg accgatgaag
    18721 aagcggttca agcgatggtt caacagatca aagaagatgt aggttcagtt gatattctag
    18781 tcaacaatgc agggatcatc aaacggacac cgatgatcga aatggatgcg gcagacttcc
    18841 gccaagtaat cgacgtggat ttaaatgccc cattcatcgt ttcaaaagca gttattccgg
    18901 atatgatcga aaaaggcggc ggtaaaatca tcaatatctg ctccatgatg agtgaattag
    18961 gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg attaaaaatg ctgacgaaaa
    19021 acatcgcttc tgaatacggt caatacaata tccaatgtaa tggaattgga ccaggttata
    19081 ttgctacacc gcaaactgct ccattacgcg aaacacaaga aaacggtgaa cgtcatccat
    19141 tcgatcaatt tatcgttgga cgtacgccag cagcacgctg gggcgagcca atcgatttgg
    19201 ctggaccatc tgtcttcttg gcttctagcg catctgattt cgttaacggc catgtcttgt
    19261 atgtggacgg aggcatactt gcctatatcg gaaaacaacc ttaaaagagc tttagtacac
    19321 tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa taccgaagga cagctgatta
    19381 aacatcattt attcgaaaaa agaggctggg acagaagcat ttaactccaa gaaataagaa
    19441 ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
    19501 gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    19561 gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc
    19621 accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg
    19681 ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa
    19741 tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg
    19801 catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg
    19861 ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt
    19921 gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg
    19981 tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat
    20041 cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa
    20101 tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag
    20161 gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc
    20221 ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc
    20281 cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg
    20341 cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat
    20401 cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc
    20461 tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg
    20521 gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg
    20581 ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct
    20641 atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag
    20701 aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga
    20761 ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc
    20821 gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat
    20881 ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc
    20941 tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac
    21001 ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa
    21061 atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc
    21121 actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc
    21181 gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc
    21241 taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa
    21301 aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt
    21361 ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat
    21421 gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg
    21481 attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc
    21541 actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac
    21601 gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc
    21661 ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat
    21721 aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt
    21781 aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc
    21841 agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat
    21901 cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta aagtcgttcc
    21961 atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt
    22021 gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa
    22081 actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc
    22141 gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt
    22201 agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact
    22261 tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc
    22321 aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc
    22381 taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc
    22441 ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg
    22501 caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt
    22561 ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc
    22621 ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc
    22681 tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt
    22741 caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc
    22801 caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga
    22861 tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat
    22921 ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg
    22981 tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt
    23041 gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca
    23101 aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg
    23161 gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccatgg gcgatttttt
    23221 ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat
    23281 aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag
    23341 gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt
    23401 gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat
    23461 agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt
    23521 ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca
    23581 tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc
    23641 tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca
    23701 cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca
    23761 tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt
    23821 ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg
    23881 ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta
    23941 aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat
    24001 gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtctt
    24061 gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa
    24121 taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc
    24181 gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac
    24241 agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc
    24301 ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga
    24361 gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga
    24421 ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc
    24481 aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga
    24541 agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg
    24601 atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg
    24661 gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa
    24721 tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata
    24781 tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga
    24841 gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt
    24901 cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac
    24961 atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat
    25021 gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt
    25081 ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga
    25141 aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt
    25201 agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta
    25261 tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca
    25321 agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa
    25381 taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat
    25441 cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac
    25501 ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat
    25561 gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct
    25621 tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat
    25681 aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac
    25741 cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa
    25801 aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta
    25861 aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca
    25921 aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc
    25981 cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt
    26041 aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg
    26101 gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct
    26161 tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt
    26221 ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga
    26281 aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca
    26341 aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc
    26401 tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt
    26461 acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct
    26521 ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc
    26581 attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca
    26641 aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct
    26701 ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa
    26761 aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta
    26821 agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg
    26881 gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa
    26941 gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg
    27001 gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac
    27061 agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat
    27121 tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt
    27181 tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc
    27241 acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg
    27301 acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat
    27361 ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta
    27421 actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac
    27481 actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat
    27541 cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct
    27601 cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac
    27661 atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg
    27721 gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag
    27781 ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca
    27841 ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca
    27901 tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata
    27961 tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta
    28021 ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca
    28081 acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa
    28141 acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata
    28201 tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg
    28261 cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag
    28321 ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg
    28381 ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc
    28441 caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga
    28501 ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg
    28561 gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg
    28621 ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga
    28681 aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat
    28741 ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca atcatcttag
    28801 gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata
    28861 aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcgccaa
    28921 tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt
    28981 atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat
    29041 ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct
    29101 cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc
    29161 cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg
    29221 cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa
    29281 ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct
    29341 agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt
    29401 tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc
    29461 gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt
    29521 agccgcaggt gtcgttcagt cttttcaaaa ttgtttgctg gaaaataata tcagtccaga
    29581 agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga
    29641 cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa
    29701 acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa
    29761 tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt
    29821 agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga
    29881 accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc
    29941 tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc
    30001 tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg
    30061 ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat
    30121 gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt
    30181 gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat
    30241 cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac
    30301 atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc
    30361 taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta
    30421 tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa
    30481 agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat
    30541 tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg
    30601 aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt
    30661 tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt
    30721 agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg
    30781 cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc
    30841 tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt
    30901 agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat
    30961 gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga
    31021 tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga
    31081 tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt
    31141 aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca
    31201 gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt
    31261 ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga
    31321 acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta
    31381 tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct
    31441 tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg
    31501 aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag atccccgaag
    31561 aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca
    31621 agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca
    31681 aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg
    31741 tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg
    31801 aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga
    31861 tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag
    31921 cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt
    31981 atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag
    32041 agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata
    32101 atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg
    32161 agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt
    32221 tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac
    32281 atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
    32341 cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa
    32401 aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc agctttttca
    32461 caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg
    32521 caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt
    32581 ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat
    32641 gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga
    32701 cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc
    32761 gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacgcgta ctataacttt
    32821 ctagccaatc ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag
    32881 tatccaaatc aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta
    32941 gtattttttc tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga
    33001 tcggtttttc caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt
    33061 ttgaaacata agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga
    33121 tcagtgcttc tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg
    33181 agattttact tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag
    33241 cttgctgttg caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat
    33301 tataacataa atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc
    33361 tgataggcat gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct
    33421 cttggatcgg tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt
    33481 ctttgaaaat gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga
    33541 tgtaaccgtg cattacttta acgacataca tggggatcac ctgctttttt cttttcatta
    33601 tacgaactac tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca
    33661 tcatccaaag aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc
    33721 ttcactaatc gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt
    33781 aattttttgt cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac
    33841 ttttggatag ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg
    33901 caatttactt ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac
    33961 cctagatgta ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga
    34021 tgaataatcc ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat
    34081 ttctccatga ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc
    34141 ggaagtgctt cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct
    34201 ccaatgaaac tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg
    34261 gctaaaaatc ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca
    34321 gcagaaccta aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt
    34381 ttcaaaacga gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt
    34441 tcttgtatcg gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt
    34501 tgttcgggat agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca
    34561 atcaccagcc attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc
    34621 ttagaagaag tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa
    34681 agaaactggt cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga
    34741 gcactccgat ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca
    34801 gctaaagcac tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata
    34861 cgtccaaatg ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt
    34921 tgttcgttta tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag
    34981 agaaaaacag ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg
    35041 tggtatcatg aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg
    35101 caaaaagaag tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt
    35161 gtcaacagcg agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct
    35221 cgcgacgcat tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat
    35281 gtcgataact atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt
    35341 ggacacatgg atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta
    35401 atcaaggatg gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca
    35461 gcttactatg cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc
    35521 ttcgtagtcg gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat
    35581 gaagaagaac cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa
    35641 aaaggaaatg tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg
    35701 aaatcttttg tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt
    35761 caagtaccaa gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa
    35821 gcaaatccag taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt
    35881 ggtaaaggcg cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca
    35941 ttgttcttag ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct
    36001 gcttatgtcc atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa
    36061 atgggcaaat tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa
    36121 gcgaacttca tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa
    36181 agtgacatcg aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta
    36241 atggaaccac attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat
    36301 gaagaccaca ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt
    36361 ttgttgaaac gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat
    36421 caagcaaacg aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat
    36481 gccatctatc gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt
    36541 ttgcagtctt gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga
    36601 ttcaattcat attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact
    36661 aatgaaagaa gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta
    36721 ataaagcttg tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa
    36781 aagaagaaga tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta
    36841 ataatcatca tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct
    36901 tcaggacgat cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca
    36961 tggatcgtca attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca
    37021 aagatacgat tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt
    37081 ctagtaattg tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca
    37141 tcaaagtagg ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt
    37201 aatttctcga gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt
    37261 aaataatagt cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga
    37321 cgaggttctt tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc
    37381 gaattattat catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg
    37441 actgttgaaa ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt
    37501 tgttccattt ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat
    37561 aatcaaagga aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg
    37621 aatatatgtt tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac
    37681 ctctcttttg aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt
    37741 gcaccttttt ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca
    37801 taataaacag tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga
    37861 acggcgaatt tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct
    37921 tcttttagaa aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg
    37981 cgatgatacc aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct
    38041 aacccgcctt taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt
    38101 ctcacttgtt actccttgtg cttattttac cacactctca tattttcttt gaaataaata
    38161 accgctacca gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt
    38221 ttatccgcag atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat
    38281 atggaagaaa aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt
    38341 tttgaacaag atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta
    38401 cagttagatg gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc
    38461 cgtgtaatgg tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa
    38521 aacgggcttt ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag
    38581 cgttttacac ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc
    38641 ccacatttat tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc
    38701 gatgctgggt ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca
    38761 atcgttcctt acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca
    38821 tgggacaacc gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa
    38881 acattaggtc atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt
    38941 tctaaagctt ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca
    39001 gcagatgata cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg
    39061 cgtatccaag atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc
    39121 gctgaaacaa acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt
    39181 gctgctcata cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat
    39241 atccatacgc atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg
    39301 attcaaacac tgaaaggttt ggataaatca acagtaaata cgatcgtata cggaaaataa
    39361 tgcgatagtg aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa
    39421 aaagaggatc aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat
    39481 aaacagaaag tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct
    39541 acgtagaaac tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt
    39601 ttatcaaacc gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag
    39661 tagaccgtga caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt
    39721 ttgtcgtgat ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa
    39781 aacaggaaat cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg
    39841 tcaagatgaa tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac
    39901 ttgctgcttt caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag
    39961 caaacgtaat cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc
    40021 tagtcgatca gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga
    40081 aatctgttga ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca
    40141 tggcagtcaa attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc
    40201 taaatgcagt cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac
    40261 cttgtgatgt cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa
    40321 cttgtacgac ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg
    40381 tcacagtccc ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca
    40441 tgaaaaaaat ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata
    40501 atggaggaaa gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg
    40561 ttattatagc cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc
    40621 ggatcaaaga tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc
    40681 taacaatcga agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc
    40741 gattgatcaa agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg
    40801 ttgtcgggta cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg
    40861 agacagaagt agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag
    40921 ctggacaaaa agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct
    40981 ggccaggaac tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc
    41041 atttgccaaa tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg
    41101 gagaagcatt tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt
    41161 aattttcact attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag
    41221 ttgttttttc tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct
    41281 tatacaaagc agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa
    41341 tgacgtcgta ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca
    41401 cgatgacacc gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag
    41461 aaacggcaga taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt
    41521 tttccagttg attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt
    41581 ctacgcttac attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga
    41641 taacttgtcc ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat
    41701 ctgtttggat cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc
    41761 ggttcaagga agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg
    41821 aaccaatcgc aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa
    41881 ctgtttcgac tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc
    41941 caactaattc agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc
    42001 catctactac gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca
    42061 cgccgcttcc ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt
    42121 gctgcccgaa tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag
    42181 aaacgacagc gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt
    42241 tgacattttc cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac
    42301 tattgttgcc agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga
    42361 tccctccaac aagccccaga cctaacttac gccagaggct attattttta ctttttttca
    42421 tctttggtgt cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta
    42481 ctgttagtat agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga
    42541 aaaactttag gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga
    42601 tactgtgtaa aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc
    42661 tgtgtataaa gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat
    42721 aaggtaaaat aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg
    42781 gaaatctttt taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata
    42841 aacaaagtga gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag
    42901 gaaaattaaa agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg
    42961 cctatgcaaa aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg
    43021 aagcgcaaat gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag
    43081 aacaagaata cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg
    43141 ctaaacaaat cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg
    43201 gctctttagg actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa
    43261 aaatgacgta tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct
    43321 tccggatcaa tgcgggggct ccataccaca aataatgata ttttttaaga gaaaaaatta
    43381 tccctatt
//
LOCUS       DADVXN010000010        41707 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.010, whole genome shotgun sequence.
ACCESSION   DADVXN010000010 DADVXN010000000
VERSION     DADVXN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.010"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB942_000503"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB942_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBB6850956.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB942_000504"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB942_000504"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBB6850957.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB942_000505"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB942_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBB6850958.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="KB942_000506"
     CDS             2179..2499
                     /locus_tag="KB942_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBB6850959.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="KB942_000507"
     CDS             complement(2611..3480)
                     /locus_tag="KB942_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6850960.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="KB942_000508"
     CDS             complement(3576..4880)
                     /locus_tag="KB942_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6850961.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="KB942_000509"
     CDS             complement(4877..5386)
                     /locus_tag="KB942_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850962.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="KB942_000510"
     CDS             complement(5561..7696)
                     /locus_tag="KB942_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6850963.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="KB942_000511"
     CDS             complement(7779..9434)
                     /locus_tag="KB942_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6850964.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="KB942_000512"
     CDS             complement(9436..9750)
                     /locus_tag="KB942_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6850965.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="KB942_000513"
     CDS             9920..10180
                     /locus_tag="KB942_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBB6850966.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="KB942_000514"
     CDS             10194..10508
                     /locus_tag="KB942_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBB6850967.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="KB942_000515"
     CDS             complement(11238..11792)
                     /locus_tag="KB942_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBB6850968.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="KB942_000516"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="KB942_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="KB942_000517"
     CDS             complement(14153..14869)
                     /locus_tag="KB942_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6850969.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB942_000518"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB942_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBB6850970.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB942_000519"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB942_000519"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBB6850971.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="KB942_000520"
     CDS             complement(18339..19799)
                     /locus_tag="KB942_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6850972.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="KB942_000521"
     CDS             complement(19789..21084)
                     /locus_tag="KB942_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6850973.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="KB942_000522"
     CDS             complement(21416..22093)
                     /locus_tag="KB942_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBB6850974.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="KB942_000523"
     CDS             complement(22123..23214)
                     /locus_tag="KB942_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBB6850975.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="KB942_000524"
     CDS             complement(23315..23749)
                     /locus_tag="KB942_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6850976.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB942_000525"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB942_000525"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6850977.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EWVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="KB942_000526"
     CDS             complement(25787..27682)
                     /locus_tag="KB942_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6850978.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="KB942_000527"
     CDS             complement(27740..29515)
                     /locus_tag="KB942_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6850979.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="KB942_000528"
     CDS             complement(29565..31061)
                     /locus_tag="KB942_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6850980.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="KB942_000529"
     CDS             complement(31255..32877)
                     /locus_tag="KB942_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6850981.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="KB942_000530"
     CDS             complement(33205..34518)
                     /locus_tag="KB942_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6850982.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="KB942_000531"
     CDS             complement(34763..34987)
                     /locus_tag="KB942_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850983.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="KB942_000532"
     CDS             complement(35007..35429)
                     /locus_tag="KB942_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBB6850984.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="KB942_000533"
     CDS             complement(35571..36281)
                     /locus_tag="KB942_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6850985.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="KB942_000534"
     CDS             complement(36299..36796)
                     /locus_tag="KB942_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6850986.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="KB942_000535"
     CDS             complement(36833..37285)
                     /locus_tag="KB942_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6850987.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="KB942_000536"
     CDS             complement(37420..38025)
                     /locus_tag="KB942_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6850988.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="KB942_000537"
     CDS             complement(38475..39095)
                     /locus_tag="KB942_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6850989.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="KB942_000538"
     CDS             complement(39097..39966)
                     /locus_tag="KB942_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBB6850990.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="KB942_000539"
     CDS             complement(39989..40396)
                     /locus_tag="KB942_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6850991.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="KB942_000540"
     CDS             complement(40415..41704)
                     /locus_tag="KB942_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6850992.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11760 a         8982 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 acccattccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DADVXN010000011        35587 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.011, whole genome shotgun sequence.
ACCESSION   DADVXN010000011 DADVXN010000000
VERSION     DADVXN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.011"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            267..2033
                     /locus_tag="KB942_000541"
     CDS             267..2033
                     /locus_tag="KB942_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBB6850993.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2135..3394
                     /locus_tag="KB942_000542"
     CDS             2135..3394
                     /locus_tag="KB942_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6850994.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3541..4842
                     /locus_tag="KB942_000543"
     CDS             3541..4842
                     /locus_tag="KB942_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6850995.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4845..5696
                     /locus_tag="KB942_000544"
     CDS             4845..5696
                     /locus_tag="KB942_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6850996.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5709..6524
                     /locus_tag="KB942_000545"
     CDS             5709..6524
                     /locus_tag="KB942_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBB6850997.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6675..7841
                     /locus_tag="KB942_000546"
     CDS             6675..7841
                     /locus_tag="KB942_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6850998.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8071..8718
                     /locus_tag="KB942_000547"
     CDS             8071..8718
                     /locus_tag="KB942_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6850999.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8802..9746
                     /locus_tag="KB942_000548"
     CDS             8802..9746
                     /locus_tag="KB942_000548"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBB6851000.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9881..11332
                     /locus_tag="KB942_000549"
     CDS             9881..11332
                     /locus_tag="KB942_000549"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBB6851001.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11353..12189
                     /locus_tag="KB942_000550"
     CDS             11353..12189
                     /locus_tag="KB942_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6851002.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12189..12848
                     /locus_tag="KB942_000551"
     CDS             12189..12848
                     /locus_tag="KB942_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6851003.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12974..13663
                     /locus_tag="KB942_000552"
     CDS             12974..13663
                     /locus_tag="KB942_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851004.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13669..14625
                     /locus_tag="KB942_000553"
     CDS             13669..14625
                     /locus_tag="KB942_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBB6851005.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14669..15181
                     /locus_tag="KB942_000554"
     CDS             14669..15181
                     /locus_tag="KB942_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851006.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15437..15985)
                     /locus_tag="KB942_000555"
     CDS             complement(15437..15985)
                     /locus_tag="KB942_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6851007.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16074..17255)
                     /locus_tag="KB942_000556"
     CDS             complement(16074..17255)
                     /locus_tag="KB942_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBB6851008.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17491..18999)
                     /locus_tag="KB942_000557"
     CDS             complement(17491..18999)
                     /locus_tag="KB942_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBB6851009.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19158..19349
                     /locus_tag="KB942_000558"
     CDS             19158..19349
                     /locus_tag="KB942_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851010.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19552..20469
                     /locus_tag="KB942_000559"
     CDS             19552..20469
                     /locus_tag="KB942_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBB6851011.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20462..21535
                     /locus_tag="KB942_000560"
     CDS             20462..21535
                     /locus_tag="KB942_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851012.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21683..22201
                     /locus_tag="KB942_000561"
     CDS             21683..22201
                     /locus_tag="KB942_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851013.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22182..22574
                     /locus_tag="KB942_000562"
     CDS             22182..22574
                     /locus_tag="KB942_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBB6851014.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22650..23585
                     /locus_tag="KB942_000563"
     CDS             22650..23585
                     /locus_tag="KB942_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBB6851015.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23718..24497
                     /locus_tag="KB942_000564"
     CDS             23718..24497
                     /locus_tag="KB942_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6851016.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24490..24807
                     /locus_tag="KB942_000565"
     CDS             24490..24807
                     /locus_tag="KB942_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6851017.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25051..26661
                     /locus_tag="KB942_000566"
     CDS             25051..26661
                     /locus_tag="KB942_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6851018.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26818..27666
                     /locus_tag="KB942_000567"
     CDS             26818..27666
                     /locus_tag="KB942_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6851019.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27897..28376
                     /locus_tag="KB942_000568"
     CDS             27897..28376
                     /locus_tag="KB942_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851020.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28409..30637
                     /locus_tag="KB942_000569"
     CDS             28409..30637
                     /locus_tag="KB942_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6851021.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30766..32409
                     /locus_tag="KB942_000570"
     CDS             30766..32409
                     /locus_tag="KB942_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6851022.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32482..33027)
                     /locus_tag="KB942_000571"
     CDS             complement(32482..33027)
                     /locus_tag="KB942_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBB6851023.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33050..33535)
                     /locus_tag="KB942_000572"
     CDS             complement(33050..33535)
                     /locus_tag="KB942_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6851024.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33525..34337)
                     /locus_tag="KB942_000573"
     CDS             complement(33525..34337)
                     /locus_tag="KB942_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBB6851025.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34476..>35587)
                     /locus_tag="KB942_000574"
     CDS             complement(34476..>35587)
                     /locus_tag="KB942_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6851026.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11648 a         6403 c         7114 g        10422 t
ORIGIN      
        1 gaatatactt ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt
       61 gatcgcttaa gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa
      121 agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt
      181 taccgataac atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt
      241 catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa
      301 gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa
      361 atgatatcaa aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat
      421 ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg
      481 aaattgaagt gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag
      541 ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta
      601 ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc
      661 cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg
      721 acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag
      781 actttttcgg gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg
      841 gcatcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata
      901 cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga
      961 ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg
     1021 gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag
     1081 actggttcca catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa
     1141 ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata
     1201 cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat
     1261 tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa
     1321 aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc
     1381 attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta
     1441 cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg
     1501 gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat
     1561 tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga
     1621 agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag
     1681 aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg
     1741 aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt
     1801 tgtctaagat tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata
     1861 agctctattt cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa
     1921 aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt
     1981 tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt
     2041 taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg
     2101 taacagcacg ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt
     2161 gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa
     2221 ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg
     2281 atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat
     2341 caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc
     2401 tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca
     2461 aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc
     2521 ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt
     2581 gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct
     2641 atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc
     2701 taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt
     2761 tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg
     2821 aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa
     2881 aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga
     2941 cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat
     3001 tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt
     3061 cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca
     3121 ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag
     3181 ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac
     3241 agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt
     3301 gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa
     3361 aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat
     3421 aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc
     3481 tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag
     3541 atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt
     3601 ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata
     3661 ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt
     3721 actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg
     3781 caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat
     3841 cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata
     3901 cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg
     3961 atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac
     4021 ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt
     4081 atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt
     4141 tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct
     4201 caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg
     4261 gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc
     4321 atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc
     4381 tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta
     4441 ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta
     4501 tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt
     4561 cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat
     4621 aatttctcca tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct
     4681 ggttctactg atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag
     4741 tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg
     4801 ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt
     4861 cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac
     4921 tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa
     4981 atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc
     5041 tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa
     5101 caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt
     5161 ttatcggccg caaatcaagt ctgatgttct ttttagtgat tcagatggta ccgactatgg
     5221 cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt
     5281 tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat
     5341 actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt
     5401 tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat
     5461 tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac
     5521 ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa
     5581 gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct
     5641 tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc
     5701 agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag
     5761 tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag
     5821 tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat
     5881 tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg
     5941 aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg
     6001 aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg
     6061 attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata
     6121 cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac
     6181 gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt
     6241 taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt
     6301 tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa
     6361 atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc
     6421 actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat
     6481 gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt
     6541 atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa
     6601 aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt
     6661 taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct
     6721 cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca
     6781 ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta
     6841 acgcatcttt ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta
     6901 tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt
     6961 atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc
     7021 atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt
     7081 attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag
     7141 tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt
     7201 gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga
     7261 tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag
     7321 aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc
     7381 atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca
     7441 aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac
     7501 aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc
     7561 ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg
     7621 aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag
     7681 tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt
     7741 tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg
     7801 ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt
     7861 taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct
     7921 aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt
     7981 ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa
     8041 tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta
     8101 tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat
     8161 acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc
     8221 acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa
     8281 gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact
     8341 agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat
     8401 caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca
     8461 gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt
     8521 gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt
     8581 acggcaacag attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta
     8641 aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca
     8701 actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat
     8761 gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct
     8821 ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta
     8881 cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta
     8941 cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta
     9001 tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt
     9061 atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg
     9121 tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg
     9181 aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg
     9241 ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat
     9301 tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc
     9361 cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac
     9421 aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca
     9481 agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa
     9541 tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg
     9601 caagcatgtt acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa
     9661 tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta
     9721 aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt
     9781 tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac
     9841 gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt
     9901 agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa
     9961 aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag
    10021 taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt
    10081 tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt
    10141 atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg
    10201 tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc
    10261 agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag
    10321 tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg
    10381 cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg
    10441 agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt
    10501 cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca
    10561 ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg
    10621 gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt
    10681 acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat
    10741 tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga
    10801 aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga
    10861 acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta
    10921 catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga
    10981 atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt
    11041 gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg
    11101 tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt
    11161 ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat
    11221 gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg
    11281 ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca
    11341 aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt
    11401 ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa
    11461 aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat
    11521 gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta
    11581 aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa
    11641 agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc
    11701 tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat
    11761 tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact
    11821 agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg
    11881 aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat
    11941 tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac
    12001 gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa
    12061 aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct
    12121 gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg
    12181 agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg
    12241 tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc
    12301 tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta
    12361 ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat
    12421 attttgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta
    12481 ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc
    12541 agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt
    12601 tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga
    12661 aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca
    12721 taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg atttattggg
    12781 tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt
    12841 caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt
    12901 gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc
    12961 ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata
    13021 ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat
    13081 tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga
    13141 gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta
    13201 taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg
    13261 aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac
    13321 atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac
    13381 aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg
    13441 cgtaaaatat tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca
    13501 agatccattt tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc
    13561 aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca
    13621 acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct
    13681 tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg
    13741 ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat
    13801 gtgatcgaat tatttcaaaa aaagcaccat cgaatcgctg tgatccaaaa tccccaagta
    13861 ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct
    13921 gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca
    13981 gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg
    14041 gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc
    14101 gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca
    14161 gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt
    14221 ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga
    14281 gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt
    14341 gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat
    14401 aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt
    14461 gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg
    14521 tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa agattggctt
    14581 aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat
    14641 gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt
    14701 agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc
    14761 tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa
    14821 agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag
    14881 cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt
    14941 atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt
    15001 cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac
    15061 ttatccagct aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg
    15121 gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg
    15181 actataaaat catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat
    15241 gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa
    15301 aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa
    15361 ctcccttcat cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga
    15421 gtattattcg atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga
    15481 acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg
    15541 ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt
    15601 ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg
    15661 tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc
    15721 ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt
    15781 ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa
    15841 aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga
    15901 tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac
    15961 cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa
    16021 agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc
    16081 agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc
    16141 aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa
    16201 atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt
    16261 gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt
    16321 gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg
    16381 ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat
    16441 atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca
    16501 gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat
    16561 gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga
    16621 tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca
    16681 tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata
    16741 gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg
    16801 gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat
    16861 ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga
    16921 atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg
    16981 gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct
    17041 tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta
    17101 ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt
    17161 agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg
    17221 acaaataaac caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg
    17281 ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt
    17341 ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa
    17401 aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt
    17461 tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt cggaagggta
    17521 gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt
    17581 gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac
    17641 ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct
    17701 tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac
    17761 taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa
    17821 gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc
    17881 tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt
    17941 atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc
    18001 ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc
    18061 gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt
    18121 atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac
    18181 agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc
    18241 ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt
    18301 gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg
    18361 aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct
    18421 ttcatgatac agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt
    18481 cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga
    18541 gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat
    18601 gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt
    18661 gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat
    18721 atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc
    18781 atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc
    18841 atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc
    18901 aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc
    18961 ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact
    19021 aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa
    19081 acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt
    19141 gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata
    19201 tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga
    19261 cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt
    19321 tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc
    19381 tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca
    19441 gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac
    19501 tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag
    19561 ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat
    19621 acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc
    19681 caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag
    19741 atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat
    19801 cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct
    19861 tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat
    19921 taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct
    19981 aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa
    20041 atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa
    20101 caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat
    20161 cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat
    20221 gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa
    20281 gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa
    20341 aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc
    20401 ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag
    20461 tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga
    20521 agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat
    20581 ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa
    20641 tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa
    20701 agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga
    20761 tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga
    20821 tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata
    20881 cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc
    20941 ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga
    21001 attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag
    21061 tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc
    21121 tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa
    21181 tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac
    21241 attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa
    21301 tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa
    21361 gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac
    21421 aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat
    21481 tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga
    21541 taagttggat taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat
    21601 ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga
    21661 tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc
    21721 tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac
    21781 tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag
    21841 tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg
    21901 gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca
    21961 aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg
    22021 tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca
    22081 aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct
    22141 gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata
    22201 gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac
    22261 aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac
    22321 aagaaaatgg tcaaaacttt tcggaaaaag aagtgttctt caaaaaaggc gatgatttac
    22381 tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg
    22441 aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa
    22501 gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt
    22561 tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg
    22621 agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt
    22681 ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg
    22741 caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca
    22801 atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc
    22861 aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct
    22921 tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg
    22981 aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa
    23041 gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga
    23101 tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt
    23161 gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga
    23221 ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca
    23281 ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt
    23341 tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa
    23401 cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca
    23461 aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc
    23521 ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta
    23581 aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct tgtttttaaa
    23641 taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta tttgttgtaa
    23701 ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg
    23761 cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga gtattgttgc
    23821 aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc ttttccggtg
    23881 gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca acgacattat
    23941 tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt gccggtttta
    24001 tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat gatgaaaact
    24061 atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga aaggctttat
    24121 atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt aattttttcg
    24181 gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc atgttcattt
    24241 tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta ttctcaggcg
    24301 tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt gcagcaacag
    24361 tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa aaagctaaga
    24421 aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg gaacaggagg
    24481 ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt catcgtggca
    24541 tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc atcctggttc
    24601 agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt taaagatgtt
    24661 ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc aatgattcta
    24721 gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac aggacttgcg
    24781 tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca gagattgcga
    24841 cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt ctggatgcaa
    24901 gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc ttttttattt
    24961 taggaattag agagggattt gtccttctat gattttttgc tataataggg aagatgttta
    25021 ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca aggaacattt
    25081 tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt gattccctgg
    25141 ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta taatgttggc
    25201 tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa tgcgattgca
    25261 aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct gatttttaga
    25321 agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat gtatttattt
    25381 gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat actagctatc
    25441 agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag aggatatttc
    25501 caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca ggctgtgcga
    25561 gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg aaccatatta
    25621 gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc gattatccat
    25681 atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt gatttccagc
    25741 cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga aacactaccg
    25801 tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt aacgatgaaa
    25861 ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt cttattcagc
    25921 cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt aggaacagta
    25981 gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat acaaatcgaa
    26041 aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc attgactgga
    26101 atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc tgaaagtgtc
    26161 ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac gatcctatct
    26221 acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt agaagcaatt
    26281 atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata cgggatgagc
    26341 ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa
    26401 gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac tttccgctcc
    26461 accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca gcgattgtca
    26521 atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat tggcggctta
    26581 gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat
    26641 catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg aaaaacggca
    26701 actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat atctaatctt
    26761 acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat taaacacatg
    26821 aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa gaaccatccc
    26881 gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa cgaaggaatc
    26941 gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt tcctactact
    27001 tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga gaaagggtat
    27061 gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa cacattattt
    27121 gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa gatcactagt
    27181 atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg tcagtccctt
    27241 gatgaaatac ttgcacatat cgatcgaatc cgagataata cctacgctta tctgattgta
    27301 gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc gctgattggc
    27361 agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt tttattcgaa
    27421 aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg tagaagaaat
    27481 aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt agaagtagcg
    27541 attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga aattggtcat
    27601 ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc catttcagcg
    27661 caataattcc ttttattcaa aaaacatacc aaaaatattc caacatcatc ttggactttc
    27721 tacatgttac taatgagcgt gtaaaagaac gagatggtgt ttttatttct atcaatgaag
    27781 ctctttttct ttgcaatcct cacttcaatc ctctataatg tcgcatatag tttagtaaat
    27841 gagtaaacta ctaatttact tatttactaa aaataaaagg atgaggagtt aaaataatgg
    27901 aaataccaac aaatttaaaa cataaaccag taatcgttgt agaagactat gataaagttg
    27961 atggaagaaa tgcattaaat acggatgcaa aagggctttc actgggcctt gctcagtgga
    28021 atgatcgtgg aaaagtcgat atctcaggca aggtatggcg acatactgga gaaaaatggt
    28081 cacgacagtc ggaagaatta ccaatcacac gtattttaga tttagcgatc ttagctgcac
    28141 aaggaagttt atacttccaa gatgcttatc gttatgaaaa attttatgat ccagaaaatc
    28201 cagtcgtaga catcattgga ttacaaggga accgaatgac ggtagaagta gataccaaaa
    28261 atccaaagat cgatgaagac atcattttgt attacgatac actacaaaaa gacggagaac
    28321 tgattgggga acgattccgt attttaaaac gtcttttaga tgagctagga tattaataga
    28381 cagagatgat caaaagggga agagaagcat ggagaatttc tcactaacag aaaaccgaac
    28441 cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc
    28501 agaagaatgg cggatcgcct ccgaaatcaa agggaattgg caagatcctg aaatgaaaat
    28561 cagtaacgta cttttggaag gagatgccgg gtcggggaag acacaattag ccaaagcact
    28621 atctgctgat ctgcaacttc cctatacaaa agtgacctgt tttgcagata tggacaaatc
    28681 agatgttttc ggagccttat tacctgtagt agattctaat aaggaacaag accaagaatt
    28741 actagaagct atttaccaaa cagatacact tgaagctgta ctgacactga tccagaacca
    28801 ttacggcatt gatccttttt ctgctaaaga aaaattagca gagctagttc aaaggatcga
    28861 agctgattat ccagatgcaa tccattacag ctattaccct tctgaaatcg ttcgagcgat
    28921 tgaaaaagga tatttattag agattcaaga gccgactgtc atccgagatg cctctgttct
    28981 tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg aatttaccga caggaatcat
    29041 tcgtcgccat ccagattgcg tcattgtgat cactacaaat cgaaattacc aaggaaatcg
    29101 cccattaaat gaatctttaa gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc
    29161 cttagaagtc atggcagaac gtgcgatggc gaaaactatg atccaaaaac aagaacttct
    29221 cttaaagatg gcagagatta tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa
    29281 aggtgtagca ggaatgcgtt cttattttta ttgggcaaat acctataagc aaggacaaga
    29341 tttactccag tcgatttatc caaaagtttt gtataaatta tccactgatt cagatgaatt
    29401 ggcaattctt gaacaagctt tgtctgacag tggattgcta ctggaacttc aggacatatt
    29461 gagaaaagaa aaatggggtc atcctcaatc tgagcataca aaaggaagaa ccattagcgc
    29521 cgaagaagca aatgaacgaa acatcactga aaatgctgaa gaaattctgc gcttagatga
    29581 aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa gagaaacaag aagaaacaat
    29641 agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa
    29701 aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa atgtctgctt ctgacatgga
    29761 agcacatgat aaggaaatca agaagcaact gaataaagaa gcgcgacaaa tcatgaagga
    29821 aacgattcat gaaaaagaag gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc
    29881 ggaagcacgt gagctacaca tgcaagtaat gccaatagta gaatcactta gccggcaaat
    29941 tttagagtta ctggataatg aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca
    30001 gcggttcaat gcaagcaaag tagcatatgg tgatttgaga aattttgaca aaaagaaccc
    30061 gccacatgag caaccttctt tagcggtagc tgtacggatc gatgaatctg ggtcaatgat
    30121 acgggatgat cggatcgaag cggcaaaaaa agcagcgatt gccattgccg aattttctaa
    30181 gaaagtcgat atcccattgt tgatttacgg agatacagca gaccgttcgt caagagaaaa
    30241 gacttctttg ttttcttata aagaatttga agacggattt caatggttaa atgaaaaatt
    30301 agtcactatg aaaccaaggc aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga
    30361 aaaattaaac agacaatccg caacaaccaa actgttgctt aatattagtg acggtcagcc
    30421 aaaagcgttg ccggattata caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa
    30481 agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct attggccaag acaaagaaga
    30541 aatcaagtcg atctacggtg aaagtcgttt tgtagatatt actgacttga atgaatttcc
    30601 caagcaaatg attcaactga ttgcccgcta tctatgataa aataaagctc aacgaacgca
    30661 cttagcagat tcgttgagct tttcgtttct agttaccggt aacaaggata cttctaaaat
    30721 agaatcggtt ataatcaaga gggaccgtta aaggagtgaa acgatatgat acaagaacaa
    30781 aaatggtgga aagaagcagt aggctatcaa atctatcctg ctagtttcaa agatagcaat
    30841 ggcgatggtc aaggagacat caatgggatc cgtgaaaaac ttaattattt gaaagatctt
    30901 ggtatcggct ttatctggat caatccgatc tatcaatctc catttgtcga taatggttat
    30961 gacgtaagtg actatcaaga tatagaagag agattcggaa cgatggaaga ttttgattta
    31021 ttgctaaaag aagcacatga attagggatc aaagtgatta tggatcttgt catcaaccat
    31081 agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc
    31141 gattattaca tttgggtcga cggggtagat ggcaaagaac cgaataattg gacatcgatt
    31201 ttcggcggtt ctgcttggga gtattctcat gaaactggac agtattatct gcatgttttt
    31261 gcaaaagaac aaccagactt aaattgggaa agcgagaaat tgaaagaaga actattcaat
    31321 atgatccgat ggtggttgga taaagggata gatggatttc gtttagatgc gatttctcat
    31381 gtaaaaaaag atgaatattc agtaaaagcc acagagaacc ctttctcacc ctttcaaaat
    31441 gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg tttttgaaga gtacgatatc
    31501 atgactgtcg gagaagcaag tggtgtcact gcagaagaag gaccacaatg ggtagggaaa
    31561 gacggttatt tcgatatgat attcgaattt gatcatatcc atatctggca gcaagaaaaa
    31621 gaagggcaat tagatgtcct taaattgaaa cttgcattaa gcgcttggca aacttcattg
    31681 gatggtatcg gatggaacgc tttatatatg gaaaaccatg atgttcctcg tgccgtttca
    31741 gtgtttggag atactcgtcc cgatttttgg gctatgtctg caaaagcaat cgctatgatg
    31801 tatttctttt tgcaaggtac gccatttatt tatcaaggac aggaaatcgg aatgacgaat
    31861 atgccctttg aatcgatcga tcaagttgat gcagtagatt cgaaaagact ttataaaagg
    31921 ctgcttgcgg aaggtaagac aagagaagaa gcattggata tcattcgtga aacaacgaga
    31981 gacaacagca ggacacccat gcaatggact tcggaacaat atgctggttt ttctacacat
    32041 gaaccttggc tgatcactaa tccaaatacc aaaacgatca atgtagaaca acaagaatat
    32101 gaaccagagt cggtcctgca attctataag aacatgatcc gcattagaca aaccaataaa
    32161 ggtctgatat acggttctta taaagaatat cttcatgaac atccacaact ttatgtctat
    32221 gaaagatatt tagaagatga acaatatctt attatggtaa acttaacaga atcacttgct
    32281 gactatgaac ttccaaaaga agcagatcag tcctggacat tactcttgtc aaattcctct
    32341 agcggagaat ttgaagctaa aggcatacta gcaccttatg aagctcggtt atataaaaca
    32401 aacaagtaaa acttcctcgt taaagttcat tagttggact ttgcgaggaa gtttttcaga
    32461 ttgctctgtc aattactcat ttcaataatt cgctctagga ccaccgatat atttaggacg
    32521 agagcgaagg gctgtcacgt gagcttgtcc aagggttttc aaacgaggtc tgacaggtac
    32581 ttggacatgt ttgatatgca taccgattga tgtgtcaccg atatcgatac cggcttgtcc
    32641 aactacgtgt tccacttcga caggatcatt gaactggtca aaagcagcaa cagaacaagc
    32701 acctccagca tgtagtgcag gaacaacaga gacaatttcg aaattttttg cttctgccac
    32761 tgttcgttct actactaatg ctcggttcaa atgttcacaa ccttggacag ctagcgcaat
    32821 ttctttagga tccaacaact cttttaacgt acggatgatc cattgaccaa cttccgcact
    32881 ggaatttttt ccaatatgtc ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag
    32941 atcacctttt ttcaagtgtg cctcagccaa tatgtcatta actatttcag tcaattggtc
    33001 ttttaattct ttttctgaca acttcatttt ctaaaacctt ctttttcaat taatattgct
    33061 tccgtgcttg aatacgttga agacttgcag ttataggaaa ggcaagtccc attccaatca
    33121 gattttggaa gatgtttcct ggtatagaag ccactccagc cggccaatca tataaaatcc
    33181 aaccggcgat accatagccg atgatcataa ctaagcttcc taataaaagc cctaatcccc
    33241 acatacgagt agaagatttg ctgaaagaac cagcaacata tccttgtaac ccgtggatca
    33301 ctaatgaaaa aactgcccac tgcggatatc ctgaaatcaa atcgatcaat agaccactgg
    33361 aagcaccaac aactaatcca cccgttggac cataaagaaa agcagcggta tagatcccgg
    33421 cctcgcataa tgtcacaaat ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa
    33481 gggcaactgt caacgcaatc agcatagcat agatcgttaa ttgcttagtt ttcatttctc
    33541 attcctcctt tttgaatacc gccagaccag acacttccgg tatgatcgtt catcagcacg
    33601 ccattagaaa tacaatgggc aacaaatttt ttactttctg ctactgcttt ggtaaaatct
    33661 gcccctaaca caagattcgc tgttattgca gaagaaaagg cacaaccggc cccgtttact
    33721 gttgtagtgt cgattttttt atgagaaaat acctcataat gatcaattga atagagaaga
    33781 tccacagctt catctccagc catccttgtg ccgcctttga tcacaatgtt ttttgttcct
    33841 gaagcagtga gttctttagc gagatggatc atttctgtta acgaagaggg aagagcttga
    33901 ccagaaagta aagcagcttc tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt
    33961 tcttcgatga gctgcgaaat atacaattga ttgatcacag cttcagtttc tttgaatgcc
    34021 aaaacagggt ctaaaatgat tgggatattt tgttttgttt ttatgaagtc tcgaacaacc
    34081 atgattgctt cacttgaatt caataaccca attttgatac catctaatga agtcatttta
    34141 tcaatcgaaa ctaactgtcg tttaagcaat tccaaagata tagattccag cataaatcgc
    34201 tcattaatat ctagtgcccc tatagaagtc aaggcgctta gtccgaatag accaaaattc
    34261 tcaaaagttt ttaaatctgt ttggattcct ccaccagcaa atggatcaga accgccaatt
    34321 gtcaagattc ttttcataac attttcctcc atgaataaga gtataacgaa attttcaaaa
    34381 aagaaaaaca gccaattggc atatttttat ctcatccaat ttaaaaaaac aaaaagaaaa
    34441 gaaagaccaa aatggatctt tctttcctta aatagttagg acttttgtga ttttaattct
    34501 ttctcgattt gaagccgttg cactttcatt gtagctgttc gcgggattgc atcaaaatca
    34561 cggatgatag ggacgttcaa gcgaggaagt tcatggactt gctcccacca tcgattccaa
    34621 tccatttctt tatcaggagc taaagcaata atcggttgtg gagaatgatt tttatccctt
    34681 acaatcacta cttctgccaa aaaatctaac gaatctaata aaaagtcttc aatagctaat
    34741 gtactattga tcgtttcgat caaatccact tgccgatctt tcaaaaatag gtggccatgc
    34801 ttatccataa aaccgtaatc tccgctatcc caccaatcgc cataaacatt ttcctggaac
    34861 cgtgcatctt ccttgtaata agtcaaagcc cgtcctttag acagtaattg tatatgacca
    34921 tctgtgtttt caggtaggac attcccaact gaatctgtaa tccgagcgct tgtcatgtct
    34981 tccagtccaa cgcccatatc tcttgcatca cttgttttca aactctctag tgtatgagcc
    35041 ttaagaatca ttggtccgca ttcactttgg ccatatactt gaagaaatac cgcatcctgt
    35101 gccatcgacg ttcgcaaaaa tgtcttcatt gtctggttgt ttatagcatc aaaagtggag
    35161 tgatagtatc ggatacccga aaaagcttct ggatgctcct tagcagtaaa acgccactga
    35221 acaaagttat tcggatgggt ctctagtgca attggccggt gagcttccaa catatgaaca
    35281 acttttgaac tttgtgcgtt tgctaaaggc atcataggaa atcccatgga catcaaagaa
    35341 gagataccaa tattgaatct ggaatgcact ggagagatgt gaaaagcgat taatttcttt
    35401 tccgaaattt ttgtgaaaac tgttttttgc catttcgtcc gccatcccat agaatgagca
    35461 gaatgacaaa taagtttagg gataccagtt gtaccagaag tatgggtcat ataagaaatt
    35521 tcatttttgt ctaaaaatga ttgagaaaca gacaaagcag gttgttccat taaataaaga
    35581 ataggaa
//
LOCUS       DADVXN010000012        35388 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.012, whole genome shotgun sequence.
ACCESSION   DADVXN010000012 DADVXN010000000
VERSION     DADVXN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.012"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            66..365
                     /locus_tag="KB942_000575"
     CDS             66..365
                     /locus_tag="KB942_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851027.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            630..1505
                     /locus_tag="KB942_000576"
     CDS             630..1505
                     /locus_tag="KB942_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851028.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1502..1720
                     /locus_tag="KB942_000577"
     CDS             1502..1720
                     /locus_tag="KB942_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851029.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1707..4334
                     /locus_tag="KB942_000578"
     CDS             1707..4334
                     /locus_tag="KB942_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851030.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4349..4567
                     /locus_tag="KB942_000579"
     CDS             4349..4567
                     /locus_tag="KB942_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851031.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6086..6400
                     /locus_tag="KB942_000580"
     CDS             6086..6400
                     /locus_tag="KB942_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851032.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6453..7226)
                     /locus_tag="KB942_000581"
     CDS             complement(6453..7226)
                     /locus_tag="KB942_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851033.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7331..7576
                     /locus_tag="KB942_000582"
     CDS             7331..7576
                     /locus_tag="KB942_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851034.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7732..8583
                     /locus_tag="KB942_000583"
     CDS             7732..8583
                     /locus_tag="KB942_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBB6851035.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            8616..9290
                     /locus_tag="KB942_000584"
                     /pseudo
     CDS             8616..9290
                     /locus_tag="KB942_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            9547..9735
                     /locus_tag="KB942_000585"
                     /pseudo
     CDS             9547..9735
                     /locus_tag="KB942_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9781..10746
                     /locus_tag="KB942_000586"
     CDS             9781..10746
                     /locus_tag="KB942_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851036.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            10768..10980
                     /locus_tag="KB942_000587"
     CDS             10768..10980
                     /locus_tag="KB942_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6851037.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11055..11531
                     /locus_tag="KB942_000588"
     CDS             11055..11531
                     /locus_tag="KB942_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBB6851038.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            11528..13336
                     /locus_tag="KB942_000589"
     CDS             11528..13336
                     /locus_tag="KB942_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBB6851039.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            13413..13628
                     /locus_tag="KB942_000590"
     CDS             13413..13628
                     /locus_tag="KB942_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBB6851040.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13720..14583
                     /locus_tag="KB942_000591"
     CDS             13720..14583
                     /locus_tag="KB942_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851041.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            14620..15156
                     /locus_tag="KB942_000592"
     CDS             14620..15156
                     /locus_tag="KB942_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBB6851042.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15170..15592
                     /locus_tag="KB942_000593"
     CDS             15170..15592
                     /locus_tag="KB942_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6851043.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            15522..17921
                     /locus_tag="KB942_000594"
     CDS             15522..17921
                     /locus_tag="KB942_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6851044.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17953..19944
                     /locus_tag="KB942_000595"
     CDS             17953..19944
                     /locus_tag="KB942_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6851045.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19967..20218
                     /locus_tag="KB942_000596"
     CDS             19967..20218
                     /locus_tag="KB942_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBB6851046.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20208..21437
                     /locus_tag="KB942_000597"
     CDS             20208..21437
                     /locus_tag="KB942_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBB6851047.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            21434..23515
                     /locus_tag="KB942_000598"
     CDS             21434..23515
                     /locus_tag="KB942_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBB6851048.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            23664..27584
                     /locus_tag="KB942_000599"
     CDS             23664..27584
                     /locus_tag="KB942_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBB6851049.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            27585..28529
                     /locus_tag="KB942_000600"
     CDS             27585..28529
                     /locus_tag="KB942_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851050.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            28533..28736
                     /locus_tag="KB942_000601"
     CDS             28533..28736
                     /locus_tag="KB942_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBB6851051.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29036..29272)
                     /locus_tag="KB942_000602"
     CDS             complement(29036..29272)
                     /locus_tag="KB942_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851052.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(29455..29841)
                     /locus_tag="KB942_000603"
     CDS             complement(29455..29841)
                     /locus_tag="KB942_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBB6851053.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(29823..31151)
                     /locus_tag="KB942_000604"
     CDS             complement(29823..31151)
                     /locus_tag="KB942_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6851054.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31112..31441)
                     /gene="mobC"
                     /locus_tag="KB942_000605"
     CDS             complement(31112..31441)
                     /gene="mobC"
                     /locus_tag="KB942_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBB6851055.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31699..32069)
                     /locus_tag="KB942_000606"
                     /pseudo
     CDS             complement(31699..32069)
                     /locus_tag="KB942_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            32214..32399
                     /locus_tag="KB942_000607"
     CDS             32214..32399
                     /locus_tag="KB942_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6851056.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            32557..32724
                     /locus_tag="KB942_000608"
     CDS             32557..32724
                     /locus_tag="KB942_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851057.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            33342..34001
                     /gene="vanR"
                     /locus_tag="KB942_000609"
     CDS             33342..34001
                     /gene="vanR"
                     /locus_tag="KB942_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6851058.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            34004..35365
                     /gene="vanS"
                     /locus_tag="KB942_000610"
     CDS             34004..35365
                     /gene="vanS"
                     /locus_tag="KB942_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6851059.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT        10027 a         8141 c         8878 g         8342 t
ORIGIN      
        1 gtatttattt gtacttataa aggtagagga gttatagaat aattaattaa aaaggagagg
       61 tttttgtgga agcaataaaa ttaatgattt cagaagaaca ggaggaaatg tttctaaagc
      121 agttttatca aattgctaca aaagctattg aaaaggctca agaaaatgcg gggatgacaa
      181 gggaatatct caatcaaaaa gaaatggcgg aatacttaaa tgtgagtgaa aactttttaa
      241 aaaatgctgt gagaaatgag ggattgcccg tagttgaatt aggggcaaga aaattttatt
      301 cgaaaaaggc agttaatgag tttatgtttg ctcgaatgaa aagaggggaa aatattggaa
      361 gatgatgaag tagaaattat aaaaaaatgc tttaaaaaaa gaaaaaaagt gttcccaaaa
      421 gtttgcagac tgggggaaca ctcaataata aagtgctgag aaaactttat ttatagctat
      481 tatatcaaaa aagaaggtga aaaggtgttt tcttctggaa atgttgtagt tgacaaactt
      541 agagaaataa attttgacgg aaatgtaatc cacacacatg gtacgatcaa ttaagaaaaa
      601 aaacgaaaaa aggtgttgag aagccctatt tgaatgcaat agttatctta gcagaaatta
      661 cctattggta tagacctaaa aaaattttta acgatgatgg tcagctactt ggttatcaaa
      721 aaaagtttct tgaagatatt ttgcaaaaat catataagca attaagcaaa aaaacaggat
      781 tatcagaacg agctataaaa gatgcaattg tgtacttaga gaaaaagggg attatcaaaa
      841 gggtttttag aaatatttat tatgagaaaa gagcagttac attatcaaat gttatgtaca
      901 ttgttttaaa tgtacataaa ttgatagaaa ttacttttcc tactttggat tgcgaaaaaa
      961 aggagtatat gccgaaaatt cgtcagaggg gtatcgaaaa aaagagatat gtctctttaa
     1021 aaaaaggtag aggtatgtac aaatttgtat atacgtatac aaagactact tcacagaaca
     1081 tatcaaagac tactctcgtt atggaagaag aggatattaa tataaagagg atttacagta
     1141 agattagtgt tctttatgac gcaaataacg aagatggtcg aaaattattt caagtgtatc
     1201 actatttgag aaacgctaat gtagatacag aagatatcag aaaaatcatt gaatatttat
     1261 ctgaaaatac tcagtatctt attgctgagt gtattataaa acaacatgaa gcatgctttt
     1321 ttcaaagaaa aactgaagaa gggttatacg attatgtaac atattttttg aatggacttc
     1381 agaaaagata taaaagtcaa tttttagaaa atgtagagcc tatagatcag ttcaaaaaag
     1441 ctttcgaaat caaagagtta cctgaaatta ctatgcataa ttggttgaaa ggagaagaat
     1501 catgattaaa aatcgcttaa tagatcaaat gtggtgtctt gatcaaaata cagatactct
     1561 cgaattaatc gagagtatct gcttcaacac agtagttctc gatttaagag ctgaaaatga
     1621 caattgtgta actcatatca taattaatca aaaaatggct cagcagctct ctgagagcct
     1681 aagaaaatgg gctgaaggag aaaactatga aagcaattaa agaaaaaatc gttagtagaa
     1741 aaataaactg gttagcactc agtgtacttg ttttaatgat tagcgttgtt tttacaagta
     1801 atattgttga agctagcatt ttcaattggt ttgatgacac aaaaagtcaa acagagtttt
     1861 tgaataattc tctttataga ccttacctaa aaaaatacaa tggagatttc cttagtcagt
     1921 tcgggatttg gcttggatgg gctgtggtaa aaggcatgtt tactgtgact gattcgattc
     1981 aaaatatgat tccagatgtg ttggatttgt tcaatttcat tgaaagtaca gggctaaata
     2041 atgtttatca atcagtcata aatacaattg tcgttggatt aatgatcctt tcattgatgt
     2101 ttgttggcta caagatgatt actggaaaag gaacaataga tttaagatct gttggaatga
     2161 atatagtgat gtctgtagcg ttaattcttt taatgccaac aatgatttct agcggtattc
     2221 aatttgccaa aatattttac aatgatgcta ctacgattac aaattcagat gacggagtcg
     2281 cttggtcatt gataaaacaa ggagtaacag atctggcata tattaataaa acagatcaat
     2341 actcaagcat tgataaaacg gaggatcgaa ataagttaac acgaaaaaat tttcaacaga
     2401 cagatttaac acaagtgttg acggataaag tgattgataa attggaaaaa gaaaatccag
     2461 ctgcggataa tcttcgctat gaattagttg aaaattctaa taatgaattt gttgccacaa
     2521 agttttctga taacttctta tcaacatttt ctgatagctt gaaatcaggt tattatcgat
     2581 atcaagcaaa tctttggggg atttcgattg ggttgacagc gttggcaatc gcctatgtgt
     2641 tcagcgcgtt tgtgatcatt acagcaatat tagaacttgc atttaaacgt gtgttaggtg
     2701 ttcttgtttt tgcgacagat attgaaacag gacaacgttc aaaagttgtt ttatccgata
     2761 ttttacaatg ttacttaaca gttggatttc aagggtttgg actatctatg tttgctatgt
     2821 ttatcaattt tttaaatgct ggacaaggta tctccacaaa tatttttata aaaacaattg
     2881 cttatatctg tgctgtgttt gtgctgataa aaggcagtgg aacagtcatg agatactttg
     2941 gtgttgatat tggactcaaa gaaggctatg ggcaacttgc ttctgctttt ggtatgggcg
     3001 caatgctgtt tagaaaagga tcaactggat ttaatcgtgc taaaggaagc ggaaatggaa
     3061 atggatcaga cagtggagaa ggagaagatc gaaaaccaga aaagaatttc ggcgaaactc
     3121 tatcaaaaaa agctggcaaa actggaagag ctttgggata tgcccatgaa cgtggattat
     3181 caggtttagc ttcagatgga gctacaatgg ctagtgaaag agcaataaaa ccatttaaga
     3241 gtatgcgtga tatggctaat gataccaaga ataagttcaa agaaggttta gatgatggta
     3301 cagtgtccgc tattaataaa aatagcaaac ctatgttagc taaaaataaa gaagatgaaa
     3361 ctggaaaata tgctgattcc atgccaacac gtctttcgga tagaaaagat ggtgctaagg
     3421 ttgaaaatgc cgatagaatt atgtctagtt ctgaacgtat gcgtgaagca atgaaaaata
     3481 atgctgaatc gaataataat cctgtaagtg caattcagca aaaagttcaa caagatattg
     3541 aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc tgaagaatta attaatcaga
     3601 agcgacaaga agctaaatac acgcctgaag caatgaatcg tgaggaaatg ttaaggaaac
     3661 gtgtagaagg gcgtacagga gctaatacgg acgaaaaaga agcgttgacc aaagaaagaa
     3721 tccaagaagc taaaaactca aataccggat tagaaaaatt ggtcaaagaa aatctacaaa
     3781 attctacaag tagtcaaggt ggccgatcag tagatgtccg agaaaatatt caaggttcat
     3841 tgaatggtca tgtcggacgg aatgttgatg taagagagaa tttacaaagc tcttcaaatg
     3901 gtcaaggtac taagacaatt gatgttcgag aaaatgtaca aaattctaca agtagtcaag
     3961 ggtttaaatc aatggatgta agagagaatg tacaaaattc tacaagtagt caagggttta
     4021 aatcaatgga tgtaagagag aatgtacaaa gttctacaag tagtcaagga ttcaaaacag
     4081 tagatgttcg ggagaacgtt caaaaagata gtggagaaat gaaagaaaaa acacaaaaaa
     4141 tcaatatcgt tgaagacaga aagtcagcga aaaaatttga ttcaaatcat gaaacagtga
     4201 ttattgatag tgaaatccgt gaaaatgata agggatcaaa accaagacga agatttacct
     4261 atgaagacaa cgagttattc agagatacac ttaatgatcc taatccgctt ttcgataatc
     4321 tattaaaaaa ataaaagggg gaattgtcat gaaattaatt ctaaagatta gtacgcatga
     4381 tttcttagaa cttgacgaat tatgtgaatc agtaaagaac gcaacaaatg aaaaaatcac
     4441 ggtcactaat tatattgaac tgatgattca agaagaatta aagatgaaac atgagataaa
     4501 aaacaacttg aaatattgtt atctagaaaa aagtgaagag ataatccatg aattaaatgg
     4561 cttttaaaaa ttgtatttga catgaattta cgacaaggaa ttagtgggga ttaagcgatt
     4621 attctcttcc actttagaga aataatgggt taaaaagcca gaatacgtag agcaatattg
     4681 acaacctata aaggagctat tgttgaaaat tgaagtaaca aaaagataaa gggaggttta
     4741 catctatcag attaaaagtg atagtacacc catgaaacct gcaaaagagt tgaacctaat
     4801 gtatatcggt ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa gtgacgaatg
     4861 aaactatgac caaattaagc aagaaaatat atcttgcaaa taaagtagta taaaaagata
     4921 ttgataaagc caatcaaaac tttgaatttc aaacgttaga taatcaaaca gatacaaaaa
     4981 atgaaatcag ttctacgcgt acgattatta aaaataataa attttcacta gaagaaaatg
     5041 gaaataaaat caaataatta ggaagaaaaa acaaagcaat tagaaaagcc tgcaagcaaa
     5101 tttgagtaat cgagatccaa tttttttaag gaagtttaac aaaaatatgt aattctatta
     5161 tcactattaa agagaaagtt ggagcggaat ataaagaaaa gagaaaagta attaaacgca
     5221 aaaaaatgta gaacttaaaa aatataaaga ggaacaaccg ttaaaattag gaatgatgtt
     5281 gtgtaaacat tataattgct atgatataaa tgaagtgtta aagaaagcaa atttaaacca
     5341 attttgtgaa cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa actatgacca
     5401 aaagtaggta ctaaattatg accgaaaata ggtccaaaat ggattttttt atgatatcaa
     5461 aaattatgaa atgattttga aagaaaacca gttggcacag ccaacttagc caccttcctg
     5521 taggaaggtg ctatcagttt tcccttatgc tcttttcttt cttttttgtt tcatatgtca
     5581 aatgccattt tgaaatgtac catcttaaga aatttatcct taaaaacaat tgcgttattg
     5641 gataaaaaga tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt tagtggctag
     5701 catatcgtgt gattaagata ctttatagtg taagttgaag gagaaaaaat atttaaaata
     5761 tacttcccat ttcagtggga agtaaaatta atcttgttgt ttctactagt aatgtatctt
     5821 tttggaaact atgtaacgat gtaaacaaga tttttttcag aagaaaaaac gataaaagtt
     5881 gtgataaacg atttaaaaaa taggagaaaa tatatcaagc gcatggtatg aaacgcacat
     5941 ttaaaaagat tcgactaact aaaaaaataa gtttacaaag cattgttgtg tattacaagt
     6001 gatctataca ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat ggacaattga
     6061 aaatgaaagt gaggaaattg gaagaatggc tgatgaaaat aaagttgttc cgcttgaaac
     6121 actagtgata aataaggaag agtttgagca actttttctt gatttttttg ttcaatatga
     6181 caggatttat gattttagtt cgtcactaga tttatttgaa ttagaggcag aaataataga
     6241 aaacagtcga gaggagaatg gtaaaatcat catttcagta gaaaaattca atgaattatt
     6301 ctcagaaaca tttgaaatag atacagatta ttatcaacca caagaagttt tccaatcatt
     6361 tagaaatcag attattcgct caggtaagac cgtaaactga aaaacaataa aagaaacgtt
     6421 gaaagaataa ttattccttc aacgtttctt ttttagaatg ttttaatgac caaagcagtt
     6481 tcattgtcag gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa ttgatcaact
     6541 aaccatgttt tactttttga attgtagata aatctaaaat taatatcgtt attatcgtgt
     6601 tgaaggggcg tctccaaata atctgctgta tattcatagt caacgtaacc tgctaataca
     6661 aaagagagta tccctttatt taatgttaga tttaatggat aatcatacga ctttattttt
     6721 ttcaacttca tattgtacat atcttcagcc ataaaagttg agttcgtgat ttcatcttgg
     6781 gtctcattaa aaaagttatc cgaataatcg cttatttttg aaaagtcttt attggcacca
     6841 gcctcaaaaa attgttcaac tttttcagta taaagttgtg ctaacgctcc tttttcaata
     6901 ttaactttaa tattttcatg tctatcaatt tgtgtgctgt tcagtaaggg aagatcagct
     6961 tttagtttta tatcgtcttg atttctttca atttcaattg gcaaaatctt ttcagtagat
     7021 tcagtttctg ataaaggaac atctagtttt ttatcatgat aataagcttc aaatccatct
     7081 ggaaccggaa aaaaatgtat tgggtaattc ttttctgagg ttgttgtact agtttccgta
     7141 cttgtagaac ttgtgtcaga agtggtacac ccgcttatag ttccgatgag taaagcgcaa
     7201 gttagaatga ttgcaaattt cttcaaaaaa attcctcctt aaaattgaat atggactaat
     7261 actagtccca ttgtagcata ccgaatcttt aagattaata agaaaaagga ctaattttag
     7321 tccaaaaggt gtgctattct tgaaagagga aaaaattatt gataaagaaa atgtaatcgg
     7381 tagcaatatt agacgagtcc gattagaaaa aggaattggt caaaccgagc tgattagaga
     7441 cctacagctt cgtaaaattg ctataactag ggaaacgttg gttaagattg aaggtggccg
     7501 ccaacacatt aagttggatc aattgaaagc catcaaagat attctacagg tgtcttatga
     7561 agatttgctt caatgactac ttgtagtcat ttcgtttgaa attattatgt tacaccctta
     7621 tgtgtcacga agaaatactg gtataataat gaatgggata tgtaacgata gggtatactg
     7681 ttatgttgcc taataaattg tggatcaaaa tacaagaatg gagggatatc cttgtataag
     7741 gcatatgaat tggattttac aaatgctgac ttaaatgcat tctcctcaag ctcgcaatca
     7801 tatacacatg tagtaaatca aatagatcac aaccaaaaat ctattaacaa gagaatagaa
     7861 aatctatttg aaagtgaaaa tgatttagtt gattattatt ctaaggacag caaaattcta
     7921 gatgcagacg aaattattaa agattggttt ccaactataa aagcagatat ttttatatct
     7981 cactctcatg ctgatgaaaa attagctata cgttttgcat catggctatt tgaaaatttt
     8041 ggtcttactg catttattga ttcttcggtt tggggataca gttctgattt attaaaaaaa
     8101 atagaccaaa aatattgcta taaggaacaa acaaaaacat atgattatga taaaagaaat
     8161 gtaacaacta gtcatgttca tatgatgctt tctacagctt taaataatat gattgatagt
     8221 acagaatgtc tgttttttct taatacacct aattcaatta gtttgagcaa tgaaatcact
     8281 aatgaacaga aatttacgta ttcgccatgg ctatatagcg aactgacgac agcttcaata
     8341 gtggaaaaaa agaatccacg attggaaagt aatcctcaaa tgagcaccga ggatgttaga
     8401 agcattataa agcactattc ttcgcaagaa aatctgcaaa taaaatattc tttaaattta
     8461 agtgaattaa cttatttacg aaaagctgat ttgaaaaaat gggctttaga tgctgtggaa
     8521 tgcactaaag gaagtaatac attagatatt ttatataaaa acaccatttt cactaatctc
     8581 taatcaattt actgaatcga aaggaaccgg gtagaatgga taagaaagtt ttaataaaga
     8641 ggttctctga agctattgaa aaaggaaatg cagcaatatt tgcaggtgca ggattatcga
     8701 tgtcacaagg atatgtaagt tggccggaac tacttaatga tcctgcaaca gaaatcgggt
     8761 tggattctaa aaaagaaaca gacttagtta ctctggctca atattataaa aatgaaaatg
     8821 gtggatcaag aggaattttg aatcagatac ttatggataa ttttggtgaa gaattagaaa
     8881 tatctgaaaa tcatcgaata ctagcaagtt tacctattga aacttattgg acaactaatt
     8941 atgatcatct aatagaaaaa tcaatccgag aagcttataa aaaccctcag gttaagaaaa
     9001 attatactca gttagcaaca actaatccaa atgtggatac aattgtttat aaaatgcatg
     9061 gtgatataga tgatgtcagt tcgacagtaa taacaagaga tgattatgaa aaatatgatg
     9121 attcgtacgc gttatttaaa gaaactttaa aaggagattt gttaacaaaa acttttctct
     9181 ttttaggatt tagttttaca gaccctaatt tagaaagaat tttatcagat ataagatggg
     9241 ttttgagaga aaaccaaaga cctcattatt gtataatgag aaaaatatta tagaaaaaat
     9301 tttaggttat cgctgaaagc cccggaaata cgggcatttc gcaggatatg agaactcaat
     9361 ttactaccaa tttactactt tcggattgag ccgcatatat gctgaaccat actgctaata
     9421 cccgccaccc caaatacggc acatcataaa ttgaatacga caccgtaaac gcggcgttcc
     9481 tctttcccgt catgggagaa caggacgccg cttttttatg cccaaataga gaaaggaggc
     9541 gacgccatgc tatttacttc cggccctgac cgggcgtttg aacggctgat gcaggaaaag
     9601 cccggtttcg atcactacga cggcggcggg gcggacgcgc cggaggattg cggcacctgc
     9661 cgattttacc gcccggattg gaaatatcaa ttctgccagt ttgcagagtg tccctatcag
     9721 ccgggcaagc tgaccgacta tgcagcggtc aaattcacag tgaaaggagc gaacaacgct
     9781 atggcagttt tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa tcaccacctg
     9841 cggaataaga acctgacctt aaaggccaag ggcctgctgt cgcagatgtt gtccttgccg
     9901 gaggattggg actataccct aaaaggactg tcctatatca accgggaaag catcgacgcg
     9961 atcaggacag cggtatggga gcttgaaaaa gctggatata tccggcgtga gcagggccgt
    10021 gacgcaaaag gcaaaatggc cgatatggtc tataccatct acgaacagcc ggtattggaa
    10081 aacccggtat tggaaaatcc aacatcggat aacccggtgt tggaaaatcc gacatcggat
    10141 aatccggcgt cggaaaatcc aacgcaatta aataaagaag tacaaagaac tgacttatca
    10201 aaaaaagaaa aaataattac agatggacaa agtacccatt ccattcctat cccatccccg
    10261 acccctgccc ctatggaggg cgaggcagcg gctacgccgc cggaacggaa aaggacagga
    10321 aaggatgagg ctgtacggat ttatcgggaa atcattttgg agaacatcga gtatgcctat
    10381 ctcattcagg acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga cctgatgctg
    10441 gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta ccctgccgag
    10501 ctggtgaaat ccaagtacat gaagctgacc ggcgaacata tccgctttgt ccttgactgt
    10561 ctgcgggaga acacaacgaa agtccgcaat atcaaacaat acctgcgggc tatgctgttc
    10621 aacgccccca gcaccatttc caactactac acggcccttg tcgcgcacga catggcacag
    10681 cccgattggg gaaagcctaa atccggcctg ccggactatt cctgcggcga gggcgaaagc
    10741 ctgtaacgag aaaaggagga cgaccctatg aagcagggcg cattgatttt tgacgagcga
    10801 acagaccgtt acgacatccg ctttgatctg gccgactatt acggcggcct gcattgcggg
    10861 gagtgtttcg acgtgatgat cggcggcaaa tggaggccca cccgcattga aatggccgaa
    10921 aactggtatc ttgtcggcat ccgggccgac gacctttccg gcctgcgggt gcggatttga
    10981 cctatatcta cctgcggcgt ctggtggttc cgccttagac gccgcttttg actttctgac
    11041 aaggagggat gcgattgcag gaagaagtta ctcaaaagac cattgccctg tccatgaaaa
    11101 cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg caacaccggg
    11161 ccaagggcaa aaacacgctg caccacggcc agcagagcct aaagcagctt aaaaagcatg
    11221 gggctgatct ctccaacatt gagatcaccg aggccaacat tggagcgttc aagccatgcg
    11281 ccaagaaata cggcgtggat tttaccttac gaaaagataa gaccacccag ccgccccact
    11341 acgttgtgat tttcaaggcc aaggatgcag ataaccttga tctggcgttt cgggagttta
    11401 ccgccaagac gctttccaag gagcagcggc cctctatccg caaggttttg tccgctgtga
    11461 agcaaaaggc ggcggcccag accaagcagc gggccaagga gaaaatcaag gaaagggggc
    11521 tggaactgtg aagcctgacg taaagaaatt gctgatcctg aacctcccgt atctgatttt
    11581 tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg gccgctggtg ctgacctgtc
    11641 ggggaagctc ctgcattttg cggatggttt tgcggcggct tttgccaacc cgctgccaag
    11701 cctgcacctg tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc ggctggtggt
    11761 gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc atggagtatg gcagcgcccg
    11821 ctgggggacg gccaaagaca tagcgcctta catcgacccc aagtttgaaa ataatatcct
    11881 gctcacccaa acggaacggc ttaccatgac cggcaggccc aaagacccca agacagcgcg
    11941 gaataaaaac gtgctggtga tcggtggcag cggttcaggg aaaacgagat ttttcgtgaa
    12001 acccaacctg atgcaatgct ttccgaccac ggactatccg acttcgtttg tggtgacaga
    12061 tccgaaaggc actttggttc tggaaaccgg caggatgttc cagcaggcgg gctaccgcat
    12121 taaaatcctg aataccatta acttttccaa gtccatgaag tacaacccct ttgtctacat
    12181 ccattcggaa aaggacgtgc taaagctggt aaataccctg atttccaaca ccaagggcga
    12241 gggggaaaag tcagcggagg atttttgggt gaagtcggaa cggttgtttt attcggccct
    12301 gatcggctat atctggtacg aggccccagc ggaggaaatg aacttctcaa cgctgctgga
    12361 aatgataaac gccagtgaag cccgcgagga cgacccggag tttcaaagcc ccgtcgatct
    12421 catgtttgag cgtttggagc agaaagaccc cgaccatttt gcggtgcgcc agtacaagaa
    12481 attcctgctg tccgcaggca agacccgttc ttccatcctg atttcatgcg gtgccagact
    12541 tgcgcccttt gacatccggg aggtacgcga gctgatggag gacgacgaaa tggagctgga
    12601 caccatcggg gacgaaaaga cggtgctgta tctcattatg agcgatacgg acacgacctt
    12661 taatttcatt ctggctatgc tccaaagcca gctaatcaac ctgctttgtg accgggctga
    12721 tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg attttggatg aatttgccaa
    12781 cattggccaa atacccaatt tcgataaact cattgccacc attcgaagcc gtgaaatatc
    12841 ggcctctatt attttgcaga gccagtcgca gctaaaagcg atttacaagg acgccgccga
    12901 gatcatttcg gataactgcg atagtgtgct tttccttagt gggcgcggca aaaatgccaa
    12961 ggagatttcc gatgcgctgg ggaaagaaac gattgacagt ttcaacacca gcgagaaccg
    13021 gggaagccaa acgtcccacg gattgaacta tcaaaaatta ggaaaggagt tgatgtccca
    13081 agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg cagctacggg gtgtgcgccc
    13141 gtttttatcg gagaaattcg atattaccaa gcatccccgg tacaaatacc ttgccgacgc
    13201 cgacaagaag aatacctttg acgtggacag atttttgact atcacgcgcc ggaaacggca
    13261 gcaagtggta acgcaggatg aagtattcga cctgtatgaa atcgacctgt cggacgagga
    13321 cgccgccgct gaataaacag cggctttttt cattccaaca acaaaatcaa aaaatcatta
    13381 tgagccggaa accggcagaa agaggtaaat ttatggcatt ttttaattcc gcagttacta
    13441 ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg cgtatggggc ggcatcaatc
    13501 tgttggaggg atacgggcag gacaatccgg gggcaaaatc gcagggtgtc aagcagcttg
    13561 tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt tcccctgctg tccgggctgc
    13621 tgggctgatc ctggcctttg tccctgtcca aacccttaac taatcgcctc cgcgtcctcc
    13681 cggccagacc gggagggcgc gggaaaggag gtgctacccg tgattagtga aatcattgaa
    13741 gaatggataa aaggcatcct catggacggt atcacgggca acctgtccgg gctgttcgat
    13801 acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc gcaggcatgg
    13861 aacagcggta ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt ccccatagcc
    13921 ggggccatac tcgcccttgt catgtgctat gagcttatcc agatgataac ggaaaagaac
    13981 aatatgcacg actttgacag ctccatgttc ttccgctgga tattcaaatc cgcgtttgca
    14041 atcctgatcg taacgaacac atggaacatc gtcatgggcg tattcgacgc cacccaaagc
    14101 gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat ccattgattt taacaccctg
    14161 ataccagacc ttgaaagcca attggaggct atgagcgtgg gcgctctttt ggggctgtgg
    14221 ttccaaaccc ttgttgtggg cctcaccatg aatatccttt ccatctgcat tttccttgtg
    14281 acctatggcc gcatggtgga aatctatgtt gtcacgtcgc tggggcctat cccccttgcc
    14341 acaatgggca acgcggaatg gcgcggcatg ggccaagggt atctgaaatc cctgctggcg
    14401 ctgggcttcc aagccttttt aatcatggtt gtggtgggta tctacgcggt gctgatacag
    14461 aatatcggtt ccgcttccga catatccggc gcaatctggg gctgtatggg ctatacggtg
    14521 ctgctgtgct tctgcctgtt caagaccggc agtatatcaa aggccgtatt cggggcgcat
    14581 tgatcccaaa atccaagcat acacaggagg aacttgttta tttcaacgta caacatcatt
    14641 tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc ggaaaatcac
    14701 tattccacca tgagcattga cgagctgtgc gccctgccgg tggaacggct ggcgtccaaa
    14761 gactgcctgc tgtttttgtg ggccacattc cccatgctgc cggaagctct gcggttaatc
    14821 aaagcgtggg gcttttcatt caagaccgtg gcttttgtat ggctgaaaca gaaccgtaaa
    14881 agccttacat ggttttacgg gctgggccgc tggacaaggg gaaacgcgga aatctgcctg
    14941 ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt tattatcagc
    15001 ccgattgaac aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt agagctggcg
    15061 ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa cccccggctg ggacgtgtgg
    15121 gggaacgagg tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa tggcttatgt
    15181 aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc ttaccaaacg
    15241 gcaactgatc tgtttttccg gggcgctggt gatcggcgtc cccttatatt ttctcacccg
    15301 tgactatctt accaacagcg cggcggccct gctcatggta tttgccatgc tgccgggctt
    15361 tttgcttgcc atgtacgagc gtcacggcca gccccttgaa gtggtgatta tgcagatcat
    15421 ccagtgctgc ttcatccagc caaaggagcg tccctatcag accgacaaca cctacaccgc
    15481 ccttgtgcgg caataccaga tggaacagga ggtaaaggcc attgtccaaa aaggaaatcc
    15541 caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt gacgcggtaa
    15601 tccgtaaata taagggcgac ggcaggcccc acacggcgca gcagagcatc ccctacgagg
    15661 tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt cttttccaag tgcattgagt
    15721 ttgcggacat cagctatcag cttgcccagc cggacacgca gacggctatc tttgaaaaac
    15781 tgtgcgacct gtataactat gtggacgcct ccattcatat ccagttttct ttcctcaatc
    15841 ggaaagttga cccggtgcag tacgccaaga gctttgagat cgcgccgcag ggggacgact
    15901 tcgacgacat ccgggccgag tacaccggca ttctgcaaaa gcagcttgca aacggcaaca
    15961 acggcatggt aaagaccaaa tacctgacat tcaccatcga ggccgaaagc gtgaaagcgg
    16021 cgcgagccag attgaagcgc atcggctttg acctgttagg ctatttcaag agcatggggg
    16081 ccgtagccca tgtgatggac ggctgggaac ggctgaacct gctgcatggc gtgtaccacc
    16141 cggacggcga gatattcaat tttgactgga agtggctggc cccgtccggc ctctccacca
    16201 aggattttat tgccccgtcc tccctgtgct tcggcaatgc aaagacattc ggcatgggcg
    16261 gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg gacgatatgc
    16321 tggccgactt cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg caggccattg
    16381 aacagaccaa ggcgatcaag accatcaagc gcaaaattac cgaccttgac gctatgaaga
    16441 ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat ggacattctt cccagcgacc
    16501 ttgccaccta cggcgaggac gccaagaagc tgctgacaaa gttgcagacc cgcaatgaac
    16561 ggctgttcca gttgacgttc cttgtcctca acgtggccga caccaagcaa aagctgaata
    16621 acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt gtccggctgg
    16681 actaccagca ggaacagggc cttgcaagca gcctgccgct gggcgtcaat cagatcaaga
    16741 tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg acgcaggagc
    16801 tttttcaggg cggcgcggcc atgtattacg gcatcaacgc caagtcccgc aacatgatta
    16861 tgctggacag gaagcaggcc cgttgcccca atgccttgaa gctcggcacc cccggcagcg
    16921 gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg cctgacgata
    16981 tttttatttc agacccggag gccgaatatt acccccttgt caaacggctc cacggacagg
    17041 taatccggct gtcgcctacc agcaaggact ttgtgaaccc gctggacatc aacctgaatt
    17101 attcggagga cgataacccg ctggccctga aatccgactt tgtgctgtcg ttctgtgagc
    17161 ttgtcatggg cggcaagaac gggctggaag ccattgaaaa gaccgtgata gaccgggctg
    17221 tgcgcgtgat ctaccggccc tatctggcag acccccgccc ggagaatatg ccgatcctgt
    17281 ccgacctcca caaagcgttg ttagatcagc atgtcccgga ggctgaccgg gtggcgcagg
    17341 cccttgacct gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc aacgtggaca
    17401 tcggcaaccg gcttgtgtcc tttgacatta aggagctggg caagcagctc aaaaagctgg
    17461 ggatgctcat tgtccaagac cagatatggg ggcgcgtcac ggccaaccga agccaaggaa
    17521 aggccacatg gtattttgcc gacgagttcc atttgctttt gaaagaggaa cagacggcgg
    17581 cctattctgc ggagatttgg aagcggttta ggaaatgggg cggcattcca acaggtgcca
    17641 cccagaacgt caaagacctg ctttcctccc cggaaattga aaatatcctt gaaaacagcg
    17701 acttcattac gctgctcaat caggcgtccg gggatagaaa aatccttgcg gaacgcttga
    17761 atttgtccac cgaacagcag aagtacatcg acaattccga acccggcgag ggccttttga
    17821 tctttgaaaa cgtggtgctg ccctttacca accccatccc gcacaacacc cagctttaca
    17881 agattatgac gacccgctta aacgaggtgg cgggagtatg acccggcgac gccacaaagg
    17941 aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc catgacccgc
    18001 gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc cagccgggag
    18061 gccgaacagg atttgtccgc gccggggccg ggcggcaccg ccgataagct gctggaacgg
    18121 gccgacgcca tccatgacgg ccataaggcc aagaaagccg cgaagcaggc cggggctgtg
    18181 gccgccgagg gggacgctgc cctgcaccgg ccatccgccc ggctccaact gtcggaggaa
    18241 gaacgggccg acccggattt acagccctat atccgaaaat cggacaaacg ggccgataag
    18301 ctggataagg cccgcgccgc catccccaag aagcgcgtcc cggtcaagga aaccgtcttt
    18361 gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg acaaacagga caaggggcct
    18421 ccctccctga aacccaatcc ggcaggccgt cctgtttccg aggtttttct attcggccac
    18481 gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca taagggggag
    18541 gaacttgccg agcggcaggc cggaaaagcc atccgcagca ccatccggca ccacaggctg
    18601 aaaccctact gggacgcgga aaaggccgag cgcaggctat tgtctgccaa cgcggagtat
    18661 ttctaccaga aatctttgcg ggataacccg gagatcgcac aggcggccag caaccccatt
    18721 tcccggttct ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc ccggacagcg
    18781 ggccagaccg caggcgcggc ggcacagggc gcggccaaga cagccggggc ctccacgaaa
    18841 gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc ccgccactgg
    18901 aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc tgcttatcat cggcggctta
    18961 cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc ctcttacttt
    19021 tccgaggata gcgatatgtt ggccgccgag gacgcctacg cggagctgga agccgagctg
    19081 caagactatc tggacaacta cgaggccacc catgactatg acgaatacca ttttgacctt
    19141 gacgagatca gccatgaccc ctatgtgctg atttccattc tgtcggcgct ccatgacggg
    19201 atatttacca tcggggaggt acagggtgat ttgtccatgc tctttgacaa gcaatatatc
    19261 ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg aaaccgatac atggacggac
    19321 gcggagggca acacccatac agacacctac caagtgccgt atgactatta catctgctac
    19381 gtcacgctgg aaaacttcga cctgtcccat gtgccagtct atatcatgga cgaggaaacg
    19441 ctaagtttct attccctgta tatgtccacg ctaggaaacc ggcctgatct gttttccggc
    19501 agccagtacc ccaacgcctc caccctgacg ccgccgacct actacgacgt acccccggag
    19561 gctttggagg atgccacctt tgccgccatg ctcaaagagg cggaaaaata tatcggttat
    19621 ccctatgtgt ggggcggcag ctctcccagc accagctttg attgcagcgg cttcatttcg
    19681 tgggtaatca atcatagtgg atggaacgtg ggacggctgg gggcgcaggg cctatataac
    19741 atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg accttgtatt tttcaaggga
    19801 acctacgaca cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa tgtgatgctg
    19861 cattgcggga acccgatttc ctacacgaac cttacttcaa actactggca atcccatttt
    19921 tacagttacg ggcgtttacc ttaacgccag aaaggagctt tcaattatgg caaccaaact
    19981 tgaccgcatc gaaaaggaca tccagaaaac caagtctaag atcgcggagt ttcagaaaca
    20041 gcttcgggag ctggaaacgc agaaaaccga acaggagaat ttgcagatca tccagcttgt
    20101 gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg gcgcaatgca
    20161 ggccgcccct gccgcaaccc cttaccatga acaggaggac aatgcccatg aagaataaga
    20221 ccagacggat ttcccgtatt ctccccgcac ttctggccgt gatgctctgc gtcacggcct
    20281 ttcccgttac cgcccttgca ggcggcaatg acccggcacc ggccccgctg ccggaggcta
    20341 caaccgaacc cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc accgccacgg
    20401 acgggacaga aaccgaggcc gaaaaagata ccggccctct gaccggcaag gacatcaccg
    20461 acctgttttc cgcgctgttc ggttccaagg tgagcattgc cgccacggac gacggcatcc
    20521 agatcaccac cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc accaccaacg
    20581 gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc acccagcttc
    20641 ccaatgggac gacggttgag gtagtcggca cagacggcga ctggattaaa attctcttgc
    20701 cggagcgcat cggctatgtc cattccgact atatgaccgt cagcgaaaag gaagtagcgg
    20761 ctaccggcga gggcggtttt tccctctcca ttgacccgga ggaaattgct tctctgctgg
    20821 aactgttcaa tggcggcggg gccagcgccg ccctgtcccc ggacgggaac ctgtcactga
    20881 ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg gaaaccaaaa
    20941 acggcaatgt gttttatctg attattgacc gggacgacga gggaaaagaa actgtccatt
    21001 tcttaaatca ggtggatgaa gccgatctgc tgacgctgat ggaggacggg gaaaccgtca
    21061 cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg aacacgaatt
    21121 gcccggtatg taaaaccaac ctgaccgagt gcagcggccc ggagccgcag gaaccccagc
    21181 cggaggaacc cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc ctgatcgtgt
    21241 tcctgctggt ggcgctggcg ggcggcggtg cggcccttta ctatttcaag ttcaaaaagc
    21301 ccaaggctga aaccaccggc agcgacgatt tgggcgagta tgattttggg gaggacgagg
    21361 atttggagga cgaggaaccc gaaaccgagt ttgacccgga agctgatttt gtgagtgaac
    21421 aggaggacga ggaatgacat ataaacttgt aatcgcggaa aaacccagcg tggcccgcag
    21481 cattgcgggc gtgatcgggg ccacggagaa acacgacggc tatttggagg ggaacggcta
    21541 tctggtgagc tggtgcatcg gacatctggt gtcctttgcc gacgccggac gctatgacga
    21601 gcgtttcaaa aaatggcgct atgaggattt gcccattctc ccggaaacat ggcagtacat
    21661 cgtccccgac gacaagaaac agcagtttga cgttctctgt tcgctgatgc agcgccccga
    21721 cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga ttttccgttt
    21781 cgtctaccag atggcgggat gccaaaagcc ctttgagcgc ctttggattt cctctatgga
    21841 ggacagtgct attaaggacg gctttgccca cctgaaaccg ggcggcgact atgatccgct
    21901 gtatcaatcg gccctgtgcc gggccaaggc cgactggctg atcggcatta acgccacccg
    21961 gcttttttcc gtcctgtacc acaagaacct gactgtgggc cgcgtccaga cgccgacctt
    22021 aaaaatgctg gtggacaggg acgccaagat taccgatttc aagaaagaga aatatcacat
    22081 cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg acgctgccga
    22141 ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt ccgtgacgcg
    22201 ggagaaaaag acggaacagc cgcccaagct ctatgacctt accaccttgc agcgggaggc
    22261 taaccggctt ttcggctata ccgccaagca gacccttgac tatgcccagg cactgtatct
    22321 aaagaagctg ctgacctatc cgagaacgga tagtcaatat cttaccgacg atatgctgcc
    22381 cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca agggcctgaa
    22441 cctttccccg cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc accacgccat
    22501 tatccccacc gctgaatttg tgaagcaggg ctttggcact ctggccgaga gtgaaaaaaa
    22561 gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg gccgggccgc atatctatga
    22621 ggccgtcacc gccacattta cctgtgccgg gcaggagttt accgccaagg gcaagaccgt
    22681 cctttccccc ggctggaaag agattgaacg ccggttccgt tcttccttaa agaccgacgc
    22741 cgacgaggac gccgaggcgg tgcgggagct gccggagctt tgcgagggcc agacctttgc
    22801 agacgtggcc gcctctgtca ccgagcattt caccacgccg cccaagccct acaccgaaga
    22861 tacgcttttg tccgctatgg aacgggccgg ggccgaggat atgccggagg atgccgagcg
    22921 caagggatta ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg tgcagatggg
    22981 ctttgtccag agaaaaggca agcagctcat tcccaccaag gacggcatca atctggctgt
    23041 ggtgctgccg gaggcgctta cttctcccca gcttactgcc gaatgggaaa accggctgac
    23101 cgagatcgcc aaggggcagg ccgatcctga cgaatttatg gacggcatcg agacgcaggc
    23161 gcgggagctg gtgcagacct attcctgtat cagtgaggaa aagcagaaat tgtttcaggc
    23221 cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag gctgtctatg agggcaagaa
    23281 aaactactac tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg accgattttt
    23341 tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca aaaccggcaa
    23401 agtcaaggta aagggcctct attccgtaaa gaccggcaaa acctacgacg ggacggtgct
    23461 tttggctgat accgggggca agtacgtcaa ctaccgcatt gaacacagga gctaaaggac
    23521 agtaacacag caagcatagg gagtgtggct acactcccgg taaaatcccc cggccgcgtc
    23581 cctgccgggc cggggatttt tgcttgttgg ggaagtcccc aaccccctat tttttgaaac
    23641 agaacagaga aaggagtgac cgcgtgaacg aagtattttc tatccaagtc cataaccggc
    23701 agtttttgga acagggcaaa caaggcgtct ggctggattt gcccaccaca accgaaacgc
    23761 tgcaagccgc cctgcgggat gttggcatta ctgccgacaa tccgcaggat ttttttatca
    23821 acgggtattc ctgcccggag gacagacacc ttgccattcc ctatgacatg gtgctggccg
    23881 ccgacgtgga cgagctgaat ttccttgccg cccggctggg ccagcttgac gccgccgaga
    23941 tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat atcggccaga
    24001 tcatcgacta caccgaaaat gtggattact atgtgcatct tcccgacgtt aggggggatg
    24061 gacagttggg cgactactac ctgaaccgtt ccggcatggt ggatatgccg gaggaatgga
    24121 aagccgggat tgatacggcg cagtttggac gccacattgc ccagcaggaa caaggcgttt
    24181 ttacccagta cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc catgagggcc
    24241 agccggtgcc ggaggaatac cgggtgatgc actaccccca gccggaagct gaccgggagg
    24301 aaccgcaggc ccagcagccg gaggcatccg aaagccccga caaagcgccg cccccggtta
    24361 tcccgatcat gctggacggc atgaacagca aagaacgcat gaaagtgatt actgaccgtc
    24421 tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta tatggcttac ttatccgcta
    24481 tgtccaagat gcacgattac agtctgaaca atacgctgct gatcgccatg cagggcggtt
    24541 cgattgtggc gggctataac aggtggcagg atgaatttga ccgccatgtg aagcggggcg
    24601 agaaaggcat taagatactg gccccggccc cgttcaaggt taagcagcag atggagaaaa
    24661 ccgacccggc cacccagcag cccgtgatcg gcgcggacgg caagcccgtg accgaagaac
    24721 aggaagtgac ggttccggcc tttaaggtgg tgacagtctt tgacatcagc cagaccgagg
    24781 gcaaggattt tcccatgccc tatatcagtg aacttaccgg cgacgtgcag cagtatcagg
    24841 atttctttgc cgcgctggaa cggacatcac cctatcccat aagcattgag ccgatttcag
    24901 acggggccaa gggccgctgc ttctacgcaa aacagcgcat tgccatcaac gagggcatga
    24961 gcgaattgca gaccctaaag accgccatcc atgagattgc ccatgccaag ctccacgcca
    25021 ttgaccggga ggcccccgcc gaggaacagg cggcccggcc agaccgcagc acccgcgagg
    25081 tacaggccga aagcgtggcc tataccgtct gccagtatta cgggctggac acgtcggact
    25141 attcttttgg ctatgtggcc gattggagca gcggcaggga gttatccgag ctgaaagcct
    25201 cccttgaaac catccgggcc acggccagcg aattgataac cgagatcgac ggccattttg
    25261 cccagcttca gcaggagcgc gaggcccggc aggaacagga ggccgtcacg gttgacgtta
    25321 ccccggaggc agcggccccg gcgcaggccg aggaagaaca ggccccggcc cttgatccgg
    25381 cagccgagcc gcttgtgact gtgctatgga gcgaaagccc ccagctaaag gacggccagc
    25441 aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca tatccggcag
    25501 aggcaggcta tgacaaaacc gcgttccgca ttgactgtat ctttcagggc gagccggaca
    25561 gctacgaggg gcggcaggat tttggagata gggacggttc cctgatcgaa catatccaga
    25621 gctaccacca attttacgcg caggacgaaa actggaaaaa gcatgtaatc gctacaaccg
    25681 gcatagaggg ctgggagcag gacaaggccc atcgggatat gctgttaggc gagtttgtcc
    25741 cctatatgaa gctgcattgc agccttgccc aaatggaaca gacggcgcag ggccgcttac
    25801 agtccgggga aaccctgacg ccggaggaaa cggcctattt gggggcgctt ctctcttatg
    25861 tgcaggagtg ccgccccatg ctcaatcagg gccagtatca gttgccggag ccgccgaaac
    25921 tggctgactt cgaccagagc ttgcaggact acaaagcaca ggtacaggcc gagatcgcgc
    25981 aggaggccgc cgcagccggt atgaccgtgg aggaatacgc ggcggctggc tatgaagcac
    26041 cccaacagga caccttttcc atctaccagc ttcgaggcgg ggaggaaacc cgcgactatc
    26101 ggtttgagcc atacgagcgt ttacaggccg cagggctggc cgtggacagt gccaactatg
    26161 accttgttta tacggcaccg ctggataaaa gcacgtcttt ggaggatatt taccgcacat
    26221 tcaacatcga ccacccggca gattttaggg gacacagcct ttccgtttcc gacgtggtgg
    26281 tgctgcacca gaatggacag gacaccgccc actatgtaga cagcttcggc tatatggaag
    26341 tgccggagtt tttgcgggaa aatcccctgc gtaccgccga actgtctacg gagcagaacg
    26401 aaaacatgat tgacggcatt atcaacaata cgccgcccga accgcagccg gaggaacccg
    26461 aacagctatc ttttgaggat gtggcagttg cttccggcca gacacaggag gaacccccgg
    26521 aggctgcgcc cccgcaaacg gctgtccgct attatcccat caatgaggga gccgcccgcc
    26581 gcgccaagga agccatgagc ttttctgact accggcccgg cagcgccacg gcggaatacc
    26641 ggcactatgt cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt gtggagccag
    26701 agtatcacga aaagatagac agcctccttg acacctacgc ccgcaagctg gccgagaaca
    26761 tgaataaggg ctatgagatt accgcccgcg tcccctctgt cctgattgcc gggccgtcca
    26821 atttcccggt acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac atggaggaat
    26881 ttcagtacat tcagggcttg cttgacaaaa tccgcagcac cggcatgggt ggtatcagcg
    26941 ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg gaagcgtcgc
    27001 aggagttgat gaaggccgtc aatgcctact accgcaagca tgggacgctg gacggatgcc
    27061 cccatctgat cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg ggctggcact
    27121 atgagaaaaa accgttccag tcctggcagc tctccaacaa taacgccgag atacgccggt
    27181 taaagggccg cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg ggatgggagt
    27241 ttgacggcgg cactgtggag atcaaccggg tggccaaccg cctgcaaatc ttctttgagg
    27301 ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc tggtcgccca
    27361 aggccggggc atggcagcgg cagttaaacg acaccacaat ccgcgtcgct gaccgtatca
    27421 agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg gcccgcaggg
    27481 agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc caagaccgaa caggccaagc
    27541 agccaccggc gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag aagtttgaaa
    27601 atgtggacgt tctggccgcg ctggaacaga tcatgcggca gaacaccgcg ttttatcaaa
    27661 gtgactttgg cattgacaaa gacattatca ggcgggatgc cgccagcgat caggccgcag
    27721 acaagaccct gctctggatg tcccggccct ccgggacgta ctgtttccgg gaacgtgatg
    27781 ttttcctgaa agacacccga cagtacaaca catggaaatt ttacggggag cagacccgtg
    27841 acaagatact ggcctacgcg gtggagctga ccggcacaca gggcggcact atccggggca
    27901 atctctatga gctggactat ccgcagcatt tccggcacgt cattgaaacc gcccagccgg
    27961 tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga catcccggag agccagcgtt
    28021 ttgacggaag ccctgaccgg gtattgggaa actttttgta ctatgaggcc cagccccatg
    28081 acccggctgt gctgcaggag gcgctacgga cggagcagca cagccggggg cgggccgtcc
    28141 ccggcgattt caaagtccac gtcaccgctt tgcatgacag ccggattgaa accgaggccc
    28201 ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa cagccccaat aaaacgcatt
    28261 tcatggtgga gatctccccg cactttgcgg cgattgcttc caccaaggat aatgaccggc
    28321 tctttgccat gctgccctat aaatccctgt gttttaccgg gatgaaaggc aggcatggcg
    28381 tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag tattcgcagg ccccggccct
    28441 ctatccgcaa gcagctatcc gacagcaaac aggccgccag cccaaaaaag gcggcggcca
    28501 gaaccaaaaa gaatgaattg gaggtataag gaatgatgca ctttaccgtg gaggaagaaa
    28561 acctgatctg tatgtaccac aatgcagacc ggcgcagaac gataaacagg ctcaccgccg
    28621 tcatgccggt tatggatggg gatatgcggg cgcaggccca gcagaccctt tccaagctgg
    28681 aacgcatgac ggatgccgac tatgacggcc agaagttcca cttcacagac gaataacgaa
    28741 cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt ttctttttgc
    28801 ctattcctac gcatttagaa cccggtattg tcggggatag gatgaagtat tcgcttgccc
    28861 ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc aatcccttaa atgcgtaggt
    28921 tgcagctata attagacccc tgaaacagat atagccgcgc agcgggtgta tctgtttcag
    28981 gggtgtgcag gtgatagccg caaagcggcg taagagggtg cagccctctt gccgtctatc
    29041 tctgttccag cgggaagctc ggcaatgctt ccagcagttt cagggaaatg cgctgccgta
    29101 tatcttcgtc aatgactttc ttctttttgc cgtttgcctg tctgatctcc accgtggaaa
    29161 gctcgttgat gtagtcagca taatgggcca acactttttc cgtggcccac ctctcaccgg
    29221 caacggcggc tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc atcctgatac
    29281 ccccgtcaga ctgatttttt ctatgatacc atattcggaa gcatggaacc agagaaaacg
    29341 gcgcttttct gctggtaaat tctacgcatt tagaacagca cttcggcggg gataggataa
    29401 aacacccaca taccccgctg accatgccgg aaaagccttg aaatataagg gcttttacag
    29461 ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc tcggcctctt tgcggcggcg
    29521 catccgtttg gagcaggccg cgcagtattt ggcccggtta gacttcggcg tgaacgtggc
    29581 cccgcactca cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg catacagggc
    29641 cgcatccagc gggaggacag cggccttaaa ccagcggcac agaagcgaat aggaaatgct
    29701 ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg cagttgccgt cacaatagtt
    29761 gcagcactca tgcgtcagct tccgggcctt gcgatattga gggtaattca ttcgtggaat
    29821 actcatagct cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc gtccacattc
    29881 tgcttgatga tgccgtattc ctgcatctgc tttttcgctt cgccgtactt ctcatacagc
    29941 cgttcctttt cctcggccaa tcgcttgtac tcggctttca gggcgtacag atcggggagc
    30001 tttttcacac cctgtccctt tagggccttt gccgcagctt catacaaaat caacgtgctt
    30061 tcatgctcac gccggaattt tgccttatcc gctgcattgc ggtattccat agccacgggc
    30121 cagagctgct tgtaggtaga aatattcttt atcagcaccg ccatatccgc aagccggtgt
    30181 tccatagctt tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc ggccactttg
    30241 ctttccaaat ccgggtaggt gtctatccca tgttcagtca ggaaattcaa agtctttgcg
    30301 gcctgcttca aattgtggag cttcgcccac ctctcatagc ctgccgactg ctgggcctta
    30361 atgttgtttt ccagatcaat ccgcaaagaa atccccttgc gctctttggg cgctctggtg
    30421 ataacgcggc ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc
    30481 ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag aaataaactt tcccggcttg
    30541 atctcatagc cctgcgcctg catgagccgc aggaaatcat caaaatcttt tgcctgcggg
    30601 atagcggcgt caatggctgc tttgagcttc gctttccagc tcgtcccttt ccgctgggcg
    30661 tcccactcgg catagctctt gcccttactc tggccgggct tcaccacgga caatccgttt
    30721 tccttgcaca gcctgtcgct ggtgcgccgg atataggcat agctctgttt gttggaaatg
    30781 tacttgcgct gatttaccat atccacggca cagaaaataa tgtgattgtg gacgtggccc
    30841 ttatcaatat gggtagtcag cacataggga tatttccctt gcagcacttc ctcggcaagc
    30901 tgcttcccga tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc
    30961 aggtggtggc ccagattatt gcccttttgg tatgcctgcg ccagcagcat ttcaaattca
    31021 atgtccgctg tttcataaga gcaggcaaag gaggccacca gcagcttgcc gtcagttttg
    31081 tccgggttct cgatgtagtc aagggctttt ttcagcgttg acttgatagg atgtatcttt
    31141 gtaactgcca tatctgcgcc agcgccccct ttatatcctc gatgtcctgt gcgtacaccg
    31201 gccccatacc gttcagcctc tttgcaatct gattgacgtt gacgccgatc ttctgtatct
    31261 cggcggtctg cgcccggatg tcgctggtgt ccatgttgac gatatagccg tcaatgagca
    31321 tcttccgggc gtaggctgaa aagttcttcg tatgcagcag cgccattttc tgttcaatca
    31381 tagcccgttc ctccggcgtc acagggacgc gcagcacgat ttttcgcttt ctgtttgcca
    31441 tgcttcaccg tcctttccat tcaggggttt ggggatttcc ccaacaagca aaacggacgc
    31501 ggcccggcag ggacggggca ggccgttttt ccgggagtgg ctacactccc tatgcttgct
    31561 acgttactgt ttctccacta acaagacgtt tccaaagccc caaactaccc gcaaaatagc
    31621 cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag
    31681 taaaacagag gatgccagtt acacatcctc cgtttcattc ttcttcggct tttcaattag
    31741 tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc agagtttcag
    31801 cttgtggctg accctttgcc gcagttcgct ttcctcccgc atgatctccg ggttaaagta
    31861 gcgttccaca ggaagcccgc aggctttaat aagctgtatg acaaccggca gactgggaat
    31921 ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt
    31981 gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca
    32041 aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca
    32101 tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg
    32161 tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact
    32221 acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact
    32281 attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca
    32341 aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt
    32401 ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt
    32461 cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc
    32521 atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg
    32581 acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat
    32641 ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata
    32701 actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg
    32761 gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg
    32821 caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga
    32881 acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac
    32941 acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg
    33001 cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa
    33061 gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg
    33121 atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat
    33181 tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga
    33241 gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac
    33301 tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac
    33361 ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat
    33421 atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt
    33481 atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg
    33541 aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa
    33601 accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca ttcaagatgc
    33661 agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa
    33721 tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag
    33781 agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca
    33841 gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt gagggtgatg
    33901 gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa aatatcatca
    33961 aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa gaaaagggat
    34021 tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg gtgtaacagc
    34081 aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac agcaaacagt
    34141 aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg atattcaaga
    34201 ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg aagataaaga
    34261 gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta gacccaactt
    34321 tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca aggcaggtgt
    34381 gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga taatggttgt
    34441 attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga tcaaagcctt
    34501 agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc tggagcgaaa
    34561 ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc tgaaagaaac
    34621 catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa cacagcgata
    34681 tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa gcgttctgtt
    34741 ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc tgcgcgaatg
    34801 catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc ttgtcagcct
    34861 gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca cggttgccga
    34921 gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg tcacagatat
    34981 tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac tatccaatgt
    35041 catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat ggagtgagcc
    35101 tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg atgatactgc
    35161 gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca gaaaaagtgg
    35221 gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga
    35281 tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga
    35341 tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgatttt
//
LOCUS       DADVXN010000013        35314 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.013, whole genome shotgun sequence.
ACCESSION   DADVXN010000013 DADVXN010000000
VERSION     DADVXN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.013"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            86..1051
                     /locus_tag="KB942_000611"
     CDS             86..1051
                     /locus_tag="KB942_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6851060.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1108..1338)
                     /locus_tag="KB942_000612"
     CDS             complement(1108..1338)
                     /locus_tag="KB942_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBB6851061.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1424..2311)
                     /locus_tag="KB942_000613"
     CDS             complement(1424..2311)
                     /locus_tag="KB942_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBB6851062.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2400..3623)
                     /locus_tag="KB942_000614"
     CDS             complement(2400..3623)
                     /locus_tag="KB942_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6851063.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3640..4686)
                     /locus_tag="KB942_000615"
     CDS             complement(3640..4686)
                     /locus_tag="KB942_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6851064.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4850..6577)
                     /gene="ptsP"
                     /locus_tag="KB942_000616"
     CDS             complement(4850..6577)
                     /gene="ptsP"
                     /locus_tag="KB942_000616"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB6851065.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6577..6843)
                     /locus_tag="KB942_000617"
     CDS             complement(6577..6843)
                     /locus_tag="KB942_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBB6851066.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7322..9556
                     /locus_tag="KB942_000618"
     CDS             7322..9556
                     /locus_tag="KB942_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6851067.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9704..10024
                     /locus_tag="KB942_000619"
     CDS             9704..10024
                     /locus_tag="KB942_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6851068.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10229..10453)
                     /locus_tag="KB942_000620"
     CDS             complement(10229..10453)
                     /locus_tag="KB942_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBB6851069.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10651..12231)
                     /locus_tag="KB942_000621"
     CDS             complement(10651..12231)
                     /locus_tag="KB942_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBB6851070.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12632..14008)
                     /locus_tag="KB942_000622"
     CDS             complement(12632..14008)
                     /locus_tag="KB942_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6851071.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14136..15254)
                     /locus_tag="KB942_000623"
     CDS             complement(14136..15254)
                     /locus_tag="KB942_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6851072.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15396..15830
                     /locus_tag="KB942_000624"
     CDS             15396..15830
                     /locus_tag="KB942_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851073.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15908..16441)
                     /locus_tag="KB942_000625"
     CDS             complement(15908..16441)
                     /locus_tag="KB942_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBB6851074.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16535..17713)
                     /gene="mutY"
                     /locus_tag="KB942_000626"
     CDS             complement(16535..17713)
                     /gene="mutY"
                     /locus_tag="KB942_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBB6851075.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17706..18503)
                     /gene="recX"
                     /locus_tag="KB942_000627"
     CDS             complement(17706..18503)
                     /gene="recX"
                     /locus_tag="KB942_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBB6851076.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18610..19992
                     /gene="rlmD"
                     /locus_tag="KB942_000628"
     CDS             18610..19992
                     /gene="rlmD"
                     /locus_tag="KB942_000628"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6851077.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20166..20744)
                     /locus_tag="KB942_000629"
     CDS             complement(20166..20744)
                     /locus_tag="KB942_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBB6851078.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21066..21536
                     /locus_tag="KB942_000630"
     CDS             21066..21536
                     /locus_tag="KB942_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBB6851079.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21644..22870)
                     /locus_tag="KB942_000631"
     CDS             complement(21644..22870)
                     /locus_tag="KB942_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6851080.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23624..23824)
                     /locus_tag="KB942_000632"
     CDS             complement(23624..23824)
                     /locus_tag="KB942_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6851081.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23958..24566)
                     /locus_tag="KB942_000633"
     CDS             complement(23958..24566)
                     /locus_tag="KB942_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBB6851082.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24651..25121)
                     /locus_tag="KB942_000634"
     CDS             complement(24651..25121)
                     /locus_tag="KB942_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBB6851083.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25169..26083)
                     /locus_tag="KB942_000635"
     CDS             complement(25169..26083)
                     /locus_tag="KB942_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6851084.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26229..27032
                     /locus_tag="KB942_000636"
     CDS             26229..27032
                     /locus_tag="KB942_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBB6851085.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27210..28157
                     /locus_tag="KB942_000637"
     CDS             27210..28157
                     /locus_tag="KB942_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBB6851086.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28225..28518)
                     /locus_tag="KB942_000638"
     CDS             complement(28225..28518)
                     /locus_tag="KB942_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBB6851087.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28546..29211)
                     /locus_tag="KB942_000639"
     CDS             complement(28546..29211)
                     /locus_tag="KB942_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBB6851088.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29351..29983)
                     /locus_tag="KB942_000640"
     CDS             complement(29351..29983)
                     /locus_tag="KB942_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6851089.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29990..31057)
                     /locus_tag="KB942_000641"
     CDS             complement(29990..31057)
                     /locus_tag="KB942_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6851090.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31054..31785)
                     /locus_tag="KB942_000642"
     CDS             complement(31054..31785)
                     /locus_tag="KB942_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBB6851091.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31954..32433)
                     /gene="greA"
                     /locus_tag="KB942_000643"
     CDS             complement(31954..32433)
                     /gene="greA"
                     /locus_tag="KB942_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBB6851092.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32569..33744)
                     /gene="mltG"
                     /locus_tag="KB942_000644"
     CDS             complement(32569..33744)
                     /gene="mltG"
                     /locus_tag="KB942_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBB6851093.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            33948..35117
                     /locus_tag="KB942_000645"
     CDS             33948..35117
                     /locus_tag="KB942_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6851094.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        10633 a         7128 c         5843 g        11710 t
ORIGIN      
        1 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
       61 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
      121 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
      181 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
      241 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
      301 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
      361 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
      421 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
      481 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
      541 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
      601 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
      661 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
      721 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
      781 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
      841 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
      901 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
      961 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
     1021 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
     1081 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
     1141 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
     1201 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
     1261 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
     1321 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
     1381 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
     1441 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
     1501 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
     1561 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
     1621 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
     1681 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
     1741 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
     1801 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
     1861 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
     1921 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
     1981 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
     2041 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
     2101 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
     2161 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
     2221 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
     2281 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
     2341 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
     2401 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
     2461 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
     2521 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
     2581 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
     2641 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
     2701 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
     2761 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
     2821 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
     2881 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
     2941 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
     3001 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
     3061 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
     3121 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
     3181 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
     3241 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
     3301 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
     3361 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
     3421 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
     3481 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
     3541 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
     3601 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
     3661 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
     3721 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
     3781 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
     3841 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
     3901 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
     3961 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
     4021 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
     4081 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
     4141 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
     4201 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
     4261 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
     4321 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
     4381 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga taaaggaatt
     4441 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
     4501 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
     4561 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
     4621 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
     4681 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
     4741 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
     4801 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
     4861 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
     4921 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
     4981 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
     5041 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
     5101 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
     5161 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
     5221 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
     5281 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
     5341 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
     5401 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
     5461 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
     5521 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
     5581 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
     5641 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
     5701 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
     5761 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
     5821 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
     5881 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
     5941 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
     6001 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
     6061 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
     6121 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
     6181 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
     6241 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
     6301 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
     6361 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
     6421 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
     6481 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
     6541 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
     6601 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
     6661 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
     6721 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
     6781 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
     6841 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
     6901 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
     6961 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
     7021 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
     7081 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
     7141 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
     7201 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
     7261 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
     7321 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
     7381 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
     7441 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
     7501 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
     7561 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
     7621 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
     7681 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
     7741 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
     7801 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
     7861 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
     7921 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
     7981 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
     8041 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
     8101 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
     8161 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
     8221 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
     8281 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
     8341 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
     8401 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
     8461 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
     8521 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
     8581 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
     8641 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
     8701 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
     8761 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
     8821 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
     8881 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
     8941 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
     9001 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
     9061 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
     9121 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
     9181 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
     9241 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
     9301 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
     9361 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
     9421 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
     9481 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
     9541 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
     9601 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
     9661 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
     9721 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
     9781 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
     9841 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
     9901 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
     9961 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    10021 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    10081 tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg gaaataagct
    10141 gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc ttggagttaa
    10201 acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt ttcttttgta
    10261 cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt tcctctttat
    10321 ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct tttacatcta
    10381 ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca cgtctttgcc
    10441 attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa aataattttt
    10501 atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc gtcacggaaa
    10561 atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat tcttattact
    10621 tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt ttacattggg
    10681 atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca acggttggtc
    10741 gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca aatcttctgg
    10801 atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg cgttatactc
    10861 atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc caatgatata
    10921 ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta attgatagat
    10981 tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca taaacagctc
    11041 tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga acaatgtatc
    11101 cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta ctgctttttc
    11161 gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca gttttttatt
    11221 tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga ttcggacaaa
    11281 agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata cgaaacctga
    11341 aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc cataaggagc
    11401 tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag tcaatgcaga
    11461 accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa atgcatctcc
    11521 agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat tttcagttag
    11581 aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt catcctgata
    11641 attttccgga cgataaaact ctacacgatt atggtagata tcataaagac cttctagccc
    11701 tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata gctcttctaa
    11761 ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga taaaagtaaa
    11821 tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg cttcgatccc
    11881 ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac gataagtatc
    11941 ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc cttgataatc
    12001 aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt ccatccagtc
    12061 tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac ggatagcccc
    12121 accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat gggaaatgat
    12181 cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca taacctaact
    12241 ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg atctcttaaa
    12301 gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt tttccgagta
    12361 aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa atacgaaaag
    12421 ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac aacctttgtc
    12481 aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc accaaaccat
    12541 aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct tgtttattca
    12601 agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt
    12661 cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa
    12721 aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat
    12781 tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat gctgtaatca
    12841 aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga
    12901 actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa
    12961 gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt
    13021 ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct
    13081 gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga acgaataaag
    13141 gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta
    13201 gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga
    13261 ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat
    13321 caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag
    13381 aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca
    13441 tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga
    13501 tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac
    13561 caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa
    13621 tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa
    13681 tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg
    13741 ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga
    13801 ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat
    13861 ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg
    13921 aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca
    13981 gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt
    14041 agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat
    14101 tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc
    14161 ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg
    14221 cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct
    14281 actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta
    14341 ggataaatga tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat
    14401 gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt
    14461 cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc
    14521 acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct
    14581 tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg
    14641 tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc
    14701 aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt
    14761 ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat gattgtcccg
    14821 taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat
    14881 tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc
    14941 aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct
    15001 gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc
    15061 aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca
    15121 aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc
    15181 atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt agactgtcta
    15241 agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa
    15301 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
    15361 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
    15421 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
    15481 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
    15541 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
    15601 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
    15661 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
    15721 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
    15781 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
    15841 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
    15901 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
    15961 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
    16021 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
    16081 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
    16141 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
    16201 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
    16261 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
    16321 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
    16381 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
    16441 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
    16501 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
    16561 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
    16621 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
    16681 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
    16741 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
    16801 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
    16861 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
    16921 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
    16981 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
    17041 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
    17101 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
    17161 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
    17221 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
    17281 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
    17341 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
    17401 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
    17461 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
    17521 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
    17581 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
    17641 cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
    17701 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
    17761 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
    17821 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
    17881 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
    17941 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
    18001 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
    18061 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
    18121 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
    18181 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
    18241 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
    18301 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
    18361 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
    18421 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
    18481 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
    18541 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
    18601 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
    18661 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
    18721 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
    18781 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
    18841 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
    18901 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
    18961 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
    19021 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
    19081 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
    19141 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
    19201 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
    19261 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
    19321 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
    19381 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
    19441 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
    19501 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
    19561 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
    19621 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
    19681 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
    19741 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
    19801 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
    19861 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
    19921 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
    19981 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
    20041 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
    20101 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
    20161 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
    20221 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
    20281 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
    20341 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
    20401 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
    20461 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
    20521 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
    20581 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
    20641 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
    20701 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
    20761 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
    20821 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
    20881 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
    20941 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
    21001 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
    21061 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
    21121 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
    21181 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
    21241 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
    21301 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
    21361 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
    21421 tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt
    21481 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
    21541 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
    21601 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
    21661 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
    21721 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
    21781 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
    21841 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
    21901 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
    21961 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
    22021 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
    22081 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
    22141 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
    22201 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
    22261 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
    22321 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
    22381 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
    22441 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
    22501 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
    22561 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
    22621 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
    22681 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
    22741 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
    22801 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
    22861 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
    22921 aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt gtgtaacgat
    22981 tattattttt taaaatttct caaaattctt aactatcata ccataccgtg aaaaagatcg
    23041 aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt ttttccagaa
    23101 aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc acataccttt
    23161 cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc acgacaattt
    23221 cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt ccagctcact
    23281 gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt taatggatag
    23341 atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg acatggtaat
    23401 gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa aactgaaatt
    23461 tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag ttaggacatg
    23521 tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa atagacaaaa
    23581 agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat ttagtcactt
    23641 cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct tgaccttctt
    23701 ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg tcatctccgc
    23761 cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc gttccgtgtt
    23821 ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa cattataccc
    23881 aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt ttaagactga
    23941 tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc atcccaatta
    24001 ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt caaataataa
    24061 gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa tggagaatct
    24121 tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca agcccatcca
    24181 gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga aaaatcacca
    24241 aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc atttggtgcc
    24301 atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg tacagctgtc
    24361 ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt tttttcgcct
    24421 aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt gtgatgctta
    24481 tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc atatgcataa
    24541 ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt cttaataatg
    24601 tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc ctagtcgtct
    24661 ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat aaacaacttg
    24721 ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc cacatactca
    24781 ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc gtaagaaagg
    24841 atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt cggtgatttt
    24901 gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca aaaccacgaa
    24961 atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc aaaactaaca
    25021 aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc ccaatatcct
    25081 tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc taccttatta
    25141 aaaattctta tttatcatac tctatatttc aataactttc atctttattt aaaaaaatta
    25201 attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta ataccatatt
    25261 ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt agtagctgat
    25321 cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc caaatatgga
    25381 catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt ttttcatagt
    25441 cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc attatcgctt
    25501 ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag tagatatcga
    25561 aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta tcacttaaca
    25621 aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt aataataata
    25681 ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa tgactattta
    25741 tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag gataatatga
    25801 tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag ccatacgtac
    25861 gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa ataattgcca
    25921 atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct gacataatcc
    25981 ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca aatagttttt
    26041 ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt atattggata
    26101 gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct gtttccttaa
    26161 agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact actagatgag
    26221 gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc aaagacctaa
    26281 aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt ttgagtataa
    26341 ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt caattggatg
    26401 ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag cttcaaaccg
    26461 aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt gatccagagg
    26521 gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt agtgtcggga
    26581 tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct gctttgttga
    26641 acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta acaggtaagc
    26701 agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc ctgacattta
    26761 tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt accaacattg
    26821 ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat tgtttaaaaa
    26881 cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg gtctttcttc
    26941 cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt gctcaagctg
    27001 tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt tatttctact
    27061 tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt ttatttgaaa
    27121 aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta tgcgctacac
    27181 ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat gcagatcgtc
    27241 catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat atctttaaaa
    27301 cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg aactcagact
    27361 accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc tcagctggtg
    27421 ttgatcagct accccttgag actttttcaa agaaacatat tttactttca aatggtagtg
    27481 gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg tattctcgtg
    27541 gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca tcggtccatt
    27601 accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt ggaaaaatga
    27661 ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca tccggtcatc
    27721 cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt cttcctgaag
    27781 tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc tttgatcagc
    27841 atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt ggtgcttctg
    27901 ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt gcagcattag
    27961 acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg gacaatgtac
    28021 tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta atggctattt
    28081 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
    28141 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga gaaggtgaca
    28201 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
    28261 gttgggatta taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
    28321 gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg gctgttcaac
    28381 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
    28441 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
    28501 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
    28561 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
    28621 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
    28681 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
    28741 aggattggag actcggattt ctgctaaaga atatttatct gacaactcta ggtaatctaa
    28801 aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct cgggatggac
    28861 gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt gggcttttgc
    28921 taaaatattt ggtacgccca tttcctttac catcagagta actaagatac tggcttgaat
    28981 atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg atcgaagtgt
    29041 catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
    29101 ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca gtgttcggca
    29161 gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca ccgttttcct
    29221 cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa gcaagcatca
    29281 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
    29341 atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct tgtgtacggt
    29401 cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt ttcagtgtaa
    29461 taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc attaaaattt
    29521 cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga ctcatttttt
    29581 ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc gttgccctta
    29641 tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct gcacctgctt
    29701 caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg attttagctt
    29761 ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct tccatgacta
    29821 agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct tgtcgtccat
    29881 tttcagcttc tccaattact tcaatgtctt cttgaataga taaataaaaa gagacgccta
    29941 agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc tatgcctcct
    30001 tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta atgattttta
    30061 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
    30121 tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa agattattgt
    30181 ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat gtatttgata
    30241 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt tttacatctt
    30301 ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat aactgctcga
    30361 taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga agcagcgcac
    30421 gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc ttgcgaaaca
    30481 tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc atcattgctg
    30541 caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt ctttctaatt
    30601 ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt acaatttgta
    30661 attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta tcaatatctt
    30721 tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg gattcataat
    30781 tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc ttttgaataa
    30841 gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac aaatggaaga
    30901 taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga ctttgattgc
    30961 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
    31021 aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct cgacatctcc
    31081 aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat cactgctgta
    31141 gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga catttcctac
    31201 taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc gtgttctgcc
    31261 tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat tgcccaaatc
    31321 tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat agacgtcgtt
    31381 tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat gtgtttttaa
    31441 ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt tggttaaatc
    31501 tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata cagccatcat
    31561 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta atatcaattg
    31621 aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat tgaaaatacc
    31681 gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
    31741 cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc tttctccctc
    31801 ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag gactttagat
    31861 gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga gtgtatcatc
    31921 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
    31981 aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac gtttgccaat
    32041 caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat ctgcttcagc
    32101 gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga atgtaactgt
    32161 tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt gagcaaaacg
    32221 aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttcgctga
    32281 ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat
    32341 gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt tttctttacc
    32401 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
    32461 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttcttc
    32521 aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct aagaattatt
    32581 tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat aaacatttcc
    32641 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
    32701 gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat tggtatacaa
    32761 attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt gttcacctaa
    32821 tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc ggttaaagaa
    32881 gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
    32941 tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat aatagttttg
    33001 catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg aagtactcgt
    33061 gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg tgttctcagc
    33121 tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa agaaagtctc
    33181 atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg ctacccgttc
    33241 agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg caacttttgt
    33301 tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag tcatattagg
    33361 cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg tataataatt
    33421 gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa tctgcttgtt
    33481 agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat ctaaaggctt
    33541 caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa taatcaataa
    33601 tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt tacgcagatt
    33661 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tatctttact
    33721 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc gtaactttat
    33781 tcattataca tgatatttga attggcgtga atgattttat ttaaatattc tatcaaaaat
    33841 gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata caagctattt
    33901 tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg aaaaaaggaa
    33961 aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg tttagcctta
    34021 gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa cactctcaga
    34081 tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat gaacaacagc
    34141 tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca atttacgtaa
    34201 agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa ctcacgcaac
    34261 aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca ttgactactc
    34321 ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag ctggctcaat
    34381 tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg tatatgcgct
    34441 caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa gtcgttcaat
    34501 ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat tcattgaact
    34561 ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa aaattattga
    34621 ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac caagtcaaaa
    34681 attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac tttaaaccac
    34741 tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc agtatgtccg
    34801 tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt tccaaagact
    34861 tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt ggggtatata
    34921 gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca tctgctatat
    34981 ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct gacaaaacta
    35041 tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa ggaactacta
    35101 agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg aatagcattt
    35161 gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat
    35221 ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct tctttctgtt
    35281 ctgtccaaat tattctaaga tttctcttta ccaa
//
LOCUS       DADVXN010000014        34771 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.014, whole genome shotgun sequence.
ACCESSION   DADVXN010000014 DADVXN010000000
VERSION     DADVXN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.014"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(227..1114)
                     /locus_tag="KB942_000646"
     CDS             complement(227..1114)
                     /locus_tag="KB942_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851095.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="KB942_000647"
     CDS             complement(1111..1980)
                     /locus_tag="KB942_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851096.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="KB942_000648"
     CDS             complement(2117..3403)
                     /locus_tag="KB942_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6851097.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="KB942_000649"
     CDS             3676..4254
                     /locus_tag="KB942_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6851098.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="KB942_000650"
     CDS             4254..5183
                     /locus_tag="KB942_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851099.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="KB942_000651"
     CDS             5180..6916
                     /locus_tag="KB942_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6851100.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="KB942_000652"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="KB942_000652"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBB6851101.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="KB942_000653"
     CDS             complement(7812..8321)
                     /locus_tag="KB942_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBB6851102.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="KB942_000654"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="KB942_000654"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBB6851103.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="KB942_000655"
     CDS             complement(9622..10713)
                     /locus_tag="KB942_000655"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBB6851104.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="KB942_000656"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="KB942_000656"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBB6851105.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="KB942_000657"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="KB942_000657"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBB6851106.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="KB942_000658"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="KB942_000658"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBB6851107.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="KB942_000659"
     CDS             complement(14676..15650)
                     /locus_tag="KB942_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBB6851108.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="KB942_000660"
     CDS             complement(15631..17514)
                     /locus_tag="KB942_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBB6851109.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="KB942_000661"
     CDS             complement(17532..19757)
                     /locus_tag="KB942_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6851110.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="KB942_000662"
     CDS             19872..20906
                     /locus_tag="KB942_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6851111.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="KB942_000663"
     CDS             complement(20973..21986)
                     /locus_tag="KB942_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6851112.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="KB942_000664"
     CDS             complement(22064..22252)
                     /locus_tag="KB942_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBB6851113.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22692..22820)
                     /locus_tag="KB942_000665"
     CDS             complement(22692..22820)
                     /locus_tag="KB942_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6851114.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="KB942_000666"
     CDS             complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="KB942_000666"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6851115.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24307..25362)
                     /locus_tag="KB942_000667"
     CDS             complement(24307..25362)
                     /locus_tag="KB942_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6851116.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="KB942_000668"
     CDS             complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="KB942_000668"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBB6851117.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="KB942_000669"
     CDS             complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="KB942_000669"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBB6851118.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="KB942_000670"
     CDS             complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="KB942_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBB6851119.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="KB942_000671"
     CDS             complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="KB942_000671"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBB6851120.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="KB942_000672"
     CDS             complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="KB942_000672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBB6851121.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33124..34683
                     /locus_tag="KB942_000673"
     CDS             33124..34683
                     /locus_tag="KB942_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBB6851122.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6336 g        11423 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaaag actaagaaac aataacaaaa gttaacactt cttagtcttt
    22681 ctttagcacg tctatttatt aaagatttta gggccttttc tcatgttttt acgatcattc
    22741 ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt tctttttgat caattctttc
    22801 attttttctt tttggtccat ttttattctc ctttaacgaa tgaagcgact aattcgatat
    22861 gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg
    22921 caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt
    22981 ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt
    23041 caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg
    23101 cttttccgac ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat
    23161 cttcaattgc ttctggaata acttccatcc catacacctt atccactctg tctgctaaag
    23221 acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact
    23281 cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa
    23341 atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc
    23401 catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt
    23461 ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt
    23521 taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac
    23581 gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt
    23641 ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga
    23701 tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta
    23761 actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg
    23821 ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat
    23881 gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg
    23941 cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa
    24001 taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta
    24061 cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc
    24121 ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta
    24181 tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac
    24241 tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt cagcaccggc
    24301 ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac cgtcgatatc
    24361 ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct ctaattcttc
    24421 ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa agaactcaat
    24481 gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac catctaagtt
    24541 gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc ttgttttggt
    24601 atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg ctgcaatatg
    24661 caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc catcatccaa
    24721 tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc ctaggaaaaa
    24781 catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt tgattggctt
    24841 tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa aaatactctt
    24901 tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag cgatattgat
    24961 gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt tttttatcac
    25021 ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat agtcgttcgt
    25081 tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga ttccattaac
    25141 cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac ctagttctgc
    25201 gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat aggcgctggc
    25261 ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt tcaataattc
    25321 ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc tctatcgttt
    25381 cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc ctttagttgc
    25441 tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt tgaaatcatc
    25501 gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt tgttcggatc
    25561 agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc catttgtgat
    25621 caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg cgatcaaacg
    25681 aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca gtttctcact
    25741 gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg catctgcacc
    25801 attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca ataccattgc
    25861 gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag ctggcatctc
    25921 aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt tcgggatatc
    25981 aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca aaactgtttt
    26041 attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg aaagaagaac
    26101 ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata aagagttcaa
    26161 gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa gagaaatatt
    26221 ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg taaattggat
    26281 aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca tatcagcttc
    26341 agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag aataaccgcc
    26401 aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga tccggatttt
    26461 tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg gttgatcaaa
    26521 ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc ggtcaaaatg
    26581 agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga attccagcgc
    26641 agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt ttgtattgct
    26701 gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt tggtttttaa
    26761 ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt ccctcctaaa
    26821 atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc cgcttgatac
    26881 atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa tccttctgat
    26941 aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat cgttaagatg
    27001 tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa tgcaactgtt
    27061 ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa atctcgtttg
    27121 atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt cataataacc cgcgcttaag
    27181 gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc tgaacgtgag
    27241 ttgacatata catcatctaa tgatttcaca ttttcagagc gataaccgta gcggatacca
    27301 tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa tgatataata aacagccaca
    27361 ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga acggaatgtt
    27421 tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc taagtattct
    27481 ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc aaaatcagga
    27541 actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc tgtcaatgct
    27601 aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga tgaagcaaag
    27661 gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac gatgccgtta
    27721 aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac tggaatttgt
    27781 ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt tgttgggtcc
    27841 caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat agcaaattca
    27901 tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc catgaccgtt
    27961 gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt caagatatct
    28021 tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt gctttcatca
    28081 atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt caatgtaatg
    28141 aatgcctcga cctcaggctc tgtttctttg attcgttcaa atgttgtttt tgtcaaatct
    28201 tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga acgattgtgt
    28261 aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact ttgataaagc
    28321 cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca gcttgcgccc
    28381 gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga acaccttccg
    28441 tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg tcggtaaaac
    28501 ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt ttcacttgtt
    28561 cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt gctctctatt
    28621 ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa aaactcattt
    28681 ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct tctgtccaaa
    28741 cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct cctgctacca
    28801 cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt tcgatcgtct
    28861 cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact gttttttctt
    28921 taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat tcgaaattga
    28981 cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg aaataagtga
    29041 ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac tcatagtcac
    29101 ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct gctttcgctc
    29161 cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct ttgctgttgt
    29221 cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac gtcaataact
    29281 cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta tcatacatct
    29341 gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa acaaagtggt
    29401 tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag gctacttctt
    29461 catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat tggtattctt
    29521 cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg atgtctcccg
    29581 ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata taatcaccgt
    29641 tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc atgatagctg
    29701 ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt agcgtttcaa
    29761 cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgata gtaaaaccaa
    29821 gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt tcatagggca
    29881 aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca atcgaatgac
    29941 atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct agcgcatcat
    30001 actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa gtgtccagat
    30061 ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg gcgttccgag
    30121 gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg acaaatgacc
    30181 gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt aatttgattg
    30241 ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt ccatccccgc
    30301 gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa agtccatcta
    30361 tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca cgttgatcaa
    30421 aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga atatcatgag
    30481 tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga gaatcagacg
    30541 tgatcaaatc aggatattgt tcctcgattg ctactaattc tgcgtattct ttatcataaa
    30601 catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat tggttcagtc
    30661 ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca tgttccatca
    30721 tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag acgttttact
    30781 ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct tactcggacg
    30841 actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca gctgttattt
    30901 tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt atagctatat
    30961 gctgtttttt tagcgaatac aggcgttttt ttaggcgtag gacgcatgcc ttcgatttcc
    31021 agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg cgtccgtcca
    31081 tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg cgtgatcccc
    31141 acatatgcga gacggcgttc ttcctccagt tcgctttctt ccatcaatgc tcgagataaa
    31201 ggaaaaaccc cctcttctag accaatcagg aatacaactg gaaattctaa tcctttggct
    31261 gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc aatgtcggaa
    31321 acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc agcgtcttct
    31381 tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa ttcctccaag
    31441 ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc tagatatccg
    31501 ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat catctgagtg
    31561 acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc accgatattt
    31621 gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga agcaaaactg
    31681 cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac gatacgctca
    31741 aaactgattg aatcttgagg atttaccagc acattcaaat acgctaaaat atctttgatt
    31801 tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt tgctttcaac
    31861 agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc aaagtcgccg
    31921 taattccgat gattgcttcg aatctcttct tgcatccggt ctacgataaa acgggtttcg
    31981 tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt ttctgtccac
    32041 aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc gctaaggatg
    32101 tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg ataatctttt
    32161 tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact ttgatctgca
    32221 tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag cgtatactgt
    32281 gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt ttgataatac
    32341 gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag gtcgtcaaag
    32401 tccatacatt gattatattg aagctctttt tggtaagcag cataacattt tgccgcaatc
    32461 tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag gctgtttttt
    32521 gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc aatgttcagt
    32581 tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat tgtaaagttc
    32641 cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat cgagtgaaaa
    32701 gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg ttctttcatt
    32761 tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt gacttctttt
    32821 tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact tcctgcacct
    32881 gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg ggggttcatc
    32941 ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat caatcttgtt
    33001 aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt tgtttctttt
    33061 tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa gcgaggcgtt
    33121 tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata ttttattcta
    33181 aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc
    33241 gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac
    33301 ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa
    33361 aatgaaccgg atatcaaggg aagcgggatt ccacaagtag agggacagct tcgcggggaa
    33421 ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt
    33481 ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga
    33541 caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc
    33601 ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt
    33661 ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca
    33721 atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt
    33781 gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta
    33841 ggcatcttag gttttattta tcagaatgtc ttgctggctt tgccaaaatt ttacaaccga
    33901 ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt
    33961 tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct
    34021 tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt
    34081 tcttacggtg ggggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta
    34141 atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag
    34201 gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca
    34261 gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg
    34321 gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca
    34381 ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact
    34441 tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat
    34501 tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac
    34561 ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg
    34621 ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt
    34681 taacgaacat ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat
    34741 gattctataa aaaaagaaga ggctgggaaa a
//
LOCUS       DADVXN010000015        33993 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.015, whole genome shotgun sequence.
ACCESSION   DADVXN010000015 DADVXN010000000
VERSION     DADVXN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.015"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(29..1261)
                     /locus_tag="KB942_000674"
     CDS             complement(29..1261)
                     /locus_tag="KB942_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBB6851123.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="KB942_000675"
     CDS             1516..2085
                     /locus_tag="KB942_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851124.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="KB942_000676"
     CDS             complement(2263..2703)
                     /locus_tag="KB942_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBB6851125.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="KB942_000677"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="KB942_000677"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBB6851126.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="KB942_000678"
     CDS             3657..4580
                     /locus_tag="KB942_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBB6851127.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="KB942_000679"
     CDS             complement(4805..5044)
                     /locus_tag="KB942_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851128.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="KB942_000680"
     CDS             complement(5049..5468)
                     /locus_tag="KB942_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6851129.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="KB942_000681"
     CDS             complement(5695..6933)
                     /locus_tag="KB942_000681"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6851130.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="KB942_000682"
     CDS             complement(7040..7675)
                     /locus_tag="KB942_000682"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851131.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="KB942_000683"
     CDS             complement(8017..8652)
                     /locus_tag="KB942_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851132.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="KB942_000684"
     CDS             9164..10306
                     /locus_tag="KB942_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBB6851133.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="KB942_000685"
     CDS             10299..10505
                     /locus_tag="KB942_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6851134.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="KB942_000686"
     CDS             10584..11777
                     /locus_tag="KB942_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6851135.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="KB942_000687"
     CDS             11982..13121
                     /locus_tag="KB942_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBB6851136.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="KB942_000688"
     CDS             13114..13914
                     /locus_tag="KB942_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6851137.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="KB942_000689"
     CDS             13914..14741
                     /locus_tag="KB942_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6851138.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="KB942_000690"
     CDS             14719..15453
                     /locus_tag="KB942_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6851139.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="KB942_000691"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="KB942_000691"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBB6851140.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="KB942_000692"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="KB942_000692"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBB6851141.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="KB942_000693"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="KB942_000693"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBB6851142.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="KB942_000694"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="KB942_000694"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBB6851143.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="KB942_000695"
     CDS             19038..21071
                     /locus_tag="KB942_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851144.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="KB942_000696"
     CDS             21115..22395
                     /locus_tag="KB942_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBB6851145.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="KB942_000697"
     CDS             22605..23411
                     /locus_tag="KB942_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851146.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="KB942_000698"
     CDS             23423..24643
                     /locus_tag="KB942_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851147.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGVNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="KB942_000699"
     CDS             24633..26219
                     /locus_tag="KB942_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6851148.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="KB942_000700"
     CDS             26258..28396
                     /locus_tag="KB942_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6851149.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="KB942_000701"
     CDS             complement(28764..29747)
                     /locus_tag="KB942_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBB6851150.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="KB942_000702"
     CDS             30125..31516
                     /locus_tag="KB942_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6851151.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="KB942_000703"
     CDS             31532..32572
                     /locus_tag="KB942_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6851152.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="KB942_000704"
     CDS             32591..33676
                     /locus_tag="KB942_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBB6851153.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..>33993
                     /locus_tag="KB942_000705"
     CDS             33709..>33993
                     /locus_tag="KB942_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6851154.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSG"
BASE COUNT        11017 a         5824 c         6701 g        10451 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggag tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggt
//
LOCUS       DADVXN010000016        33402 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.016, whole genome shotgun sequence.
ACCESSION   DADVXN010000016 DADVXN010000000
VERSION     DADVXN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.016"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            260..1816
                     /gene="rny"
                     /locus_tag="KB942_000706"
     CDS             260..1816
                     /gene="rny"
                     /locus_tag="KB942_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBB6851155.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1895..2701)
                     /gene="proC"
                     /locus_tag="KB942_000707"
     CDS             complement(1895..2701)
                     /gene="proC"
                     /locus_tag="KB942_000707"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBB6851156.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2842..5415
                     /gene="mprF"
                     /locus_tag="KB942_000708"
     CDS             2842..5415
                     /gene="mprF"
                     /locus_tag="KB942_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6851157.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5557..6420
                     /locus_tag="KB942_000709"
     CDS             5557..6420
                     /locus_tag="KB942_000709"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBB6851158.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            6665..7603
                     /locus_tag="KB942_000710"
     CDS             6665..7603
                     /locus_tag="KB942_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6851159.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7626..8306
                     /locus_tag="KB942_000711"
     CDS             7626..8306
                     /locus_tag="KB942_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6851160.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8279..9103
                     /locus_tag="KB942_000712"
     CDS             8279..9103
                     /locus_tag="KB942_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6851161.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9270..10088
                     /gene="purR"
                     /locus_tag="KB942_000713"
     CDS             9270..10088
                     /gene="purR"
                     /locus_tag="KB942_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBB6851162.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10591..11964
                     /gene="glmU"
                     /locus_tag="KB942_000714"
     CDS             10591..11964
                     /gene="glmU"
                     /locus_tag="KB942_000714"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBB6851163.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            12058..13029
                     /locus_tag="KB942_000715"
     CDS             12058..13029
                     /locus_tag="KB942_000715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6851164.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      13035..13137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13203..13772
                     /gene="thiT"
                     /locus_tag="KB942_000716"
     CDS             13203..13772
                     /gene="thiT"
                     /locus_tag="KB942_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBB6851165.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13853..14626)
                     /locus_tag="KB942_000717"
     CDS             complement(13853..14626)
                     /locus_tag="KB942_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851166.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14893..16593
                     /locus_tag="KB942_000718"
     CDS             14893..16593
                     /locus_tag="KB942_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBB6851167.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16563..16781
                     /locus_tag="KB942_000719"
     CDS             16563..16781
                     /locus_tag="KB942_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBB6851168.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16801..17691
                     /locus_tag="KB942_000720"
     CDS             16801..17691
                     /locus_tag="KB942_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBB6851169.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17709..17993
                     /locus_tag="KB942_000721"
     CDS             17709..17993
                     /locus_tag="KB942_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851170.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18480..19094
                     /gene="gmk"
                     /locus_tag="KB942_000722"
     CDS             18480..19094
                     /gene="gmk"
                     /locus_tag="KB942_000722"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBB6851171.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19099..19404
                     /locus_tag="KB942_000723"
     CDS             19099..19404
                     /locus_tag="KB942_000723"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6851172.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19657..22065
                     /gene="priA"
                     /locus_tag="KB942_000724"
     CDS             19657..22065
                     /gene="priA"
                     /locus_tag="KB942_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBB6851173.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22080..22571
                     /gene="def"
                     /locus_tag="KB942_000725"
     CDS             22080..22571
                     /gene="def"
                     /locus_tag="KB942_000725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6851174.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22564..23502
                     /locus_tag="KB942_000726"
     CDS             22564..23502
                     /locus_tag="KB942_000726"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBB6851175.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23502..24860
                     /gene="rsmB"
                     /locus_tag="KB942_000727"
     CDS             23502..24860
                     /gene="rsmB"
                     /locus_tag="KB942_000727"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBB6851176.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24873..25613
                     /locus_tag="KB942_000728"
     CDS             24873..25613
                     /locus_tag="KB942_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBB6851177.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25610..27679
                     /gene="pknB"
                     /locus_tag="KB942_000729"
     CDS             25610..27679
                     /gene="pknB"
                     /locus_tag="KB942_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBB6851178.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27844..28743
                     /gene="rsgA"
                     /locus_tag="KB942_000730"
     CDS             27844..28743
                     /gene="rsgA"
                     /locus_tag="KB942_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBB6851179.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28756..29406
                     /locus_tag="KB942_000731"
     CDS             28756..29406
                     /locus_tag="KB942_000731"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBB6851180.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29407..30045
                     /locus_tag="KB942_000732"
     CDS             29407..30045
                     /locus_tag="KB942_000732"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBB6851181.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30200..31054
                     /gene="mreC"
                     /locus_tag="KB942_000733"
     CDS             30200..31054
                     /gene="mreC"
                     /locus_tag="KB942_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBB6851182.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            31058..31567
                     /gene="mreD"
                     /locus_tag="KB942_000734"
     CDS             31058..31567
                     /gene="mreD"
                     /locus_tag="KB942_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBB6851183.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31822..33396
                     /locus_tag="KB942_000735"
     CDS             31822..33396
                     /locus_tag="KB942_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6851184.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        11009 a         5973 c         7226 g         9194 t
ORIGIN      
        1 tagaaaagtt tctcaattta aaggtttacc tatatgaaac tagtgtcatt tattttaaaa
       61 accatcgttt aatgaataag tagaaagccg tttctttgtt tatttaaagt tgacactacg
      121 gggtacaaac attagaataa agttgtgcat gttttttgta cacatatatg cgaagatgca
      181 tattgacgag tttagaataa tttatcatag aagctgtata gatgataaaa atgaaaaaga
      241 tagtatggag gtggatttta tggtattcaa cattctcctc gctatcatcg gtttaattgt
      301 cggtcttggt ttagggttta tggtcgcaaa gtcacgtcac gagaaagaaa tagctggtgc
      361 acaatcatca gctgctggaa tcatcgatag tgcaaaaaaa gaagcagaaa ctctaaaaaa
      421 agaagcattg ttagaagcta aggaagaaaa tcagaaatac cgttctgaag ttgaaagtga
      481 gctgagagaa agtaagttag aattaaaatc tcaagagaat cgtttgattc aacgcgaaca
      541 aacgctgaat cgtaaagatg actcgttaga aaaacgcgag aactcacttg aaagtaaaga
      601 agaagcactt agttcaaaac agcaattaat tgaagagcga gaaaaagacg ttgaaaaaat
      661 gattgaagat caacaaaaag aactagaaaa aattgctggt ttatcaaaag aggacgcacg
      721 agatgtcatc atgaaatcga ctgaagaaga gctgaatcat gagttaactc taatggtaaa
      781 agaatctgaa cagcgtgcca aagaagaagc agatcgcaaa gcgaaaaact tattatcatt
      841 agcgattcaa cgctgcgcag cagatcaagt ttcagaaacg actgtttcag tcgttaccct
      901 tccgaatgat gaaatgaaag gtcgtatcat tggacgagaa ggtcgtaata ttcgaacgct
      961 tgagacgctg actggtatcg atttaatcat cgatgataca ccggaagcag ttgttctttc
     1021 cggattcgat ccaatccgaa gagaaatcgc acggatgacc ttggaaaaac tgatacaaga
     1081 tggacgaatc catccagcgc gtatcgaaga aatggtcgag aaatctcgta aagaaatgga
     1141 tgaacgtatt cgcgaatacg gggaagaggc agcgtttgaa gtcggtgctc atacacttca
     1201 tccagatcta atcaagatca tcggacgtct gcgattcaga actagttatg gacaaaacgt
     1261 cttgaagcat tcggtcgagg ttgcaaaatt agctggtatc ttagcagcag agcttggcga
     1321 agatatccaa ttagcaaaac gtgccgggtt gctccatgat attgggaaag cacttgacca
     1381 tgagattgaa ggatctcacg ttgagatcgg tgcggaatta gctgccaagt acaaagaaaa
     1441 cccagtagta atcaacgcga tcgcttctca tcatggagat gtggaagcta cttctgttat
     1501 ctctgtcctt gtagctgcag cagatgcgct ctcagcagca agacctggtg cgcgaagtga
     1561 atcattagaa aactacatcc gtcgtttaag aagtctggaa aatatttcga acagcttcgc
     1621 cggtgtagaa tcaagttacg cagttcaagc tggacgcgaa gttcgggtaa tggtcaagcc
     1681 agaagaaatc tcggatttag acgctgttcg tttagtacgt gatattcgta aacgaatcga
     1741 agaagaactg gattatcctg gacacatcaa agtaatcgtt attcgggaaa ctcgtgcaac
     1801 tgattatgct aaataataaa aaatacaaaa gaagagccgc ggcaaaagcc acagctcttc
     1861 tttttttgta aggcacgaaa aaacattgct atcattattt tttattcaac atctcaagat
     1921 ctttctcgat cgtcgtattg attcctttga tgattgctgg gatgaatcct gcttcttcta
     1981 aggcaactac acctgcaaca gttgtaccgc caggagaaga aacttggtct acaagttccc
     2041 atggattttt atcacttgaa aggaattttt gacaagaacc cataattgct tttgcagcaa
     2101 tctcagtggc tgtctgtttg gacaaaccat tcagtacacc agtccgtccc atcgcatcga
     2161 tcagcatgta aacgaatgcg ggtgagctgc cagcaaggcc aatgaatgta ctgaaatctg
     2221 attctgctaa aggatagacg ctgccgattg cttcaaaaat catcgtagct tgctgaagct
     2281 gttcctccgt ggcagcttca tttttgcaaa tcgcagaaac gcctgctcct acgctgacat
     2341 tgacattcgg catcacacgt acgatggagg cctgaggatc ttcaagcgct gtttctaaat
     2401 ctattaatga ttttcctgaa gcaatagaaa tgaataagac tttctggcta tattctctta
     2461 gttcttccaa tactttttca atgatttttg gtttcacggc taaaagaacg atatctgatt
     2521 gtgatgcaac ttcctgatta cttttgcatg catggacgcc agtttcagca gcaaattttt
     2581 gtgcattttc aaaatgtcca ctggaaacga acacctcttc tttcgagact gttccttttt
     2641 ttaataaacc atcaataata gcttgtgcca tattccctgt gccgataaat cctactttca
     2701 tgttatcctc tcctttagca ttaggatatc tttctttttc tatcatcgca agcaaagaac
     2761 aggtctaaag tggaaaaatt aaaataaata agataacatt ggggtatgac acaaaagtat
     2821 acaagttagg aatgggaaaa tgtgaaaaat aaacgatact tgatgctgca atggataaag
     2881 gaacatagtc tgattttaaa actgattttt ttcggctccg tgttagtctt tgtggcaaat
     2941 caagtcgcaa atattgctaa tggcatgagt tggcaagata tttggcaaag gatgggacaa
     3001 caaagccgca tgacgatcct gctcatgatt ctggctggac tgctgggtgt cactccgatg
     3061 ttgttgtatg attgggtgac gatcagagtc ttagaaaaac aaggaaaacc aaaaatggaa
     3121 agaaaagagt ttcttcttgc tgcgtgggta acaaacacga tcaacaatct cgccggtttt
     3181 ggtggtgtgg taggagcaag tctgcgggct agtttttatg gaaagcagac aaatagaaaa
     3241 atggttttag ccactgtatc aaaagtcgca ctatttatgc tgactggttt gtctgtatgg
     3301 tcgtttctta catttattga tgtctttttt attcagtcag aaagtatttt tcgaagctat
     3361 tggatctggc ttttcggcgg tagtttttta gcaccagtca ttttcttttt tgcttatcta
     3421 aagagaaaga cattgttctt cgagttttat ccaaaaggct tgcttggtct gctactggca
     3481 tcctttggac aatggacggg agcccttgcg gtatttttga tcatcggcaa gttgatgaga
     3541 atagatattt cgatgctttc gatctatcct atgttcatga ttgctacatt gataggaatg
     3601 ctgacgatgg tcccaggtgg aatggggact tttgacgttt tgatgatctt aggaatgagt
     3661 caattgtctg ttcggcaaga tgtagcagta gtttggctat tatattatcg gttgttttat
     3721 tatgttctac cttttatgac gggacttcta ttgttcatca cacatacagg agtcaaaatc
     3781 aatcgatttt tagataacat tccgcgactg tttttccaaa aggttgcgca tatcgtgctc
     3841 gtaagtgctg tctattttgc ggggatcatg atggtacttc tatcaacaat cactaatctg
     3901 tctgcaatca gtcggctatt tcaagtatta ttgccgttct cattcaattt tttagatcag
     3961 acagtgaatc tattggttgg ctttttatta ctcggccttg ctagagcact ttcagtaaaa
     4021 gtcaaaaagg catttctgcc tacgattgct ttactatctt ttggtatttt aaatacaatc
     4081 acacgtacgt tatcttggca gttgatattg gtatatgtct tgattctgtt agctgtgtgg
     4141 ttatccagaa aagaatttta tcgaacaaaa ttcgtttatt cttggggagc tataatcttt
     4201 gatgctagct tgttcggctt tttgtttatc gtttacgcaa ttgcaggcta tcacagcgga
     4261 cgctttttag tcggtcgaat aacaggaaat cattttattt tgtttccttc agatgatgtg
     4321 tggttctcag ggcttattgg tttgggcatc tcattgctag ctttagtggc gctttatcaa
     4381 tatttagctg aagatactac gaagcttggc agtgagtacg aaaaagaccg ttttgaccgt
     4441 ttattggaaa catacggagg cacagctaca agccatcagc ttcgcttgtc aggttacagt
     4501 tatttttatt accaagaaga cgaccaagat caagtcgttt ttggttatca gatcaaagga
     4561 aacaaatgct ttgttttagg agatccagtt ggcaatccta aaatgatgcg ggcagcaacg
     4621 aatcagttta tgaaacaagc agattgttta ggctatcagc ttgcgtttta taagatatca
     4681 gaaagctacg tagtgatgct tcatgaattg ggttttcact tcacgaaagt aggtgaagca
     4741 gggattgttg atctagcaac agaagacttg tctcggctga gtcagcaact tgaatatcaa
     4801 cgtttgatga aagaaggata ccattttact tggtacgatc aactgccgga agaattacat
     4861 caagctgtaa atgaagtttc acaaaaatgg cttcaaggtg cacgagaaaa atacttctct
     4921 gtgggacgat ttgataaatc atatgtatta tctagcggtg tcggtattgt ccgtaaagat
     4981 caaaaagtcg ttggcttcat caccgctcag ccgatgacga agaagcaggc gggatatgat
     5041 ttgttgcggg tcttacctaa agaatctgag gaattagctg actatctgtt agccaatctg
     5101 tttattgtat acgagaaaat gggctactcg gaagccaatc taggcttagc tccattagct
     5161 aatgttggag aaacagattt ttctttcttt caagaaaaag tcatgcatat cgtatataat
     5221 tatggtaatt ttttctattc cttccagtct gcttatgaaa agaaattaaa atatgtcgat
     5281 cattgggaag gacgttattt tgcttatagg aaaggaagta atttcggatt tgctaccatg
     5341 cagctactca gtctgatagg aaaagaaaaa ggaaatgttc cgagtttggc agaagaagtg
     5401 ctcacagaat tataagaatc actagaactt gtttggatta tgaagagaaa attctgtgaa
     5461 agctgttgaa acgatgacaa aatcaaccac taggcagaca taagctatac ttttattttt
     5521 ccctatgata gaatacttgc gacttagact tttcgaatgg gtgagaggat ggaattgatt
     5581 gaaaaagcgc cagccaagat aaatttagga ctggatgtat tatatagacg gcaagatggg
     5641 tatcatgagt tggaaatggt gatgtcgagc gtagacttgg cagatcatct attttttgaa
     5701 gaaataacag aagacaaaat cattgtagaa acaaataaag catttttacc ggtagatgaa
     5761 cggaacaatg tttatcaagc tgctttattg ctaaaaaata aatacaatat caaaaccggt
     5821 gtcaagatct ttgttaagaa gaatattcca gtagcagctg gattaggcgg aggaagtaca
     5881 gactgtgcag ctgctctaag agggatcaac caattatgga atctgggctg ttcgttagaa
     5941 gaactggcag aaatcggttt ggaagtcggt acagatgttc cttactgtgt ctacggaagt
     6001 acggcatttg tcggtggcaa aggggaagaa atccaagtat taccttctat gccacagtgc
     6061 tgggttgtgt tggtcaaacc aagaatgagt gtctctacac gtacagtgtt tagagaagta
     6121 gatacgcaag ccatccaaca tcaagacatc cacggacttg ctgaagccat ccgagagcaa
     6181 gattatcagc agatgatcaa aaaaatggga aacagtttag aagatataac gggaaagcgg
     6241 catccagtca tccatcaaat caaagagcgt atgatcaagt acggagcaga tgctgcattg
     6301 atgagtggaa gcggaccaac cgtttttgcc ttgtgtcaac attattcaag agcgcagaga
     6361 gtatataatg ggttaaaagg tttttgtgag gaagtttatt tagtacgaac cctgcaataa
     6421 aatataagaa gtaaaatagg tgatgaaaaa ctgaggaatc ctaaaatggt attcttcagt
     6481 tttttattgt agtgaaatgg ctagatatga gcagcaactc atccatttta cgatttaatc
     6541 aagaatatac atgcttaaat cagaaattga tggatttcta ttgactgtgt ttttttattt
     6601 tgatacatta gttcacgtta ataaatcgta aaattccgat ttgttcattg aaaggagcat
     6661 ataaatgaaa aaatatcttt tgacagctac ggtaatgata ggtgctttac tgtttgcagc
     6721 atgtggaaat acgaacaagg aagcagacaa aaaagaggat ttgacgatcg ttacgacttt
     6781 ttatccgatg tatgatttta caaaagagat cgtaggggat gaaggaaacg tgaaactttt
     6841 gatccctgct ggaacggagc cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat
     6901 ttccgatgca gatgttttcg tgtataatag ttcagacatg gaattttttg tggattcgtt
     6961 aaaggattca gtggatagca aacagacctt gatgatcgaa gcagctaaag gaatcgaccg
     7021 tttagaatcg caagaagcag acgaacatga agaatcagaa gaagggcatg gtcattcaca
     7081 tgaatacgat ccacatgttt ggctcgatcc ggtattagct ataaaagaag tcagaacgat
     7141 tgctgaagaa ttaggcgaaa aatatccaga taaaaaggaa acttttacga agaatgccga
     7201 tgcttatatc aaaaagcttg aagcactgga tcaaaaatac tccgatgagc taaaagatgc
     7261 aacaaaccgc acatttgtca ctcagcatgc tgcttttgct tatttagcga atcaatacga
     7321 cttaaaacaa gtagcgattt cgggcgtttc accagatcag gaacccacac caagtcgctt
     7381 agctgagcta aaagaatttg tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa
     7441 tgcctcttcg aaggtagctg agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt
     7501 gaatccttta gaaagcttga ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt
     7561 catggaaaaa aatctgaaag ctttaaaaga aagtatcaat taagttttag aatggaggtt
     7621 gtaaggtgcg ttatatcgaa atatctgatt tgacctttta ctatgatgat gaacctgtac
     7681 tagaagatgt ctcttatcat gtagatgcgg gtgaatttgt cattttaact ggagaaaatg
     7741 gagcagccaa gtcaaccttg gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa
     7801 tgataaagat tgctgagaaa aatcaagaag gaaaaaaatt aagtatcggt tatattcctc
     7861 aacaagttgc atctttcaat gcaggttttc ctagtacggt gatcgaatta gtccgttcag
     7921 gaagatatcc aagaggccgc tggttcaagc gtctctctga aaaagatcat caacatgtac
     7981 agcgtgcttt agaagctgta gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt
     8041 ctggcggaca aaagcagcga atcagtttag cgcgtatttt tgctactgac cctgacttat
     8101 ttgttcttga tgaaccaaca acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc
     8161 ttctccgaca tagtgcgcat gtccatggga aatcagttct aatgatcacc catgatcatg
     8221 aagatatcaa acagtatgcg gacagacaga tccgactggt gagaaaggag gattctcaat
     8281 ggcgttgctt tcatatgaat tcatgagaag agcgtttctt gccgctatct tcattgccgg
     8341 aatcgccccg atgctaggtg tgtttttagt gatcagaaga caatcgctga tggctgatac
     8401 gctttctcat gtctctttag caggggtagc gttaggattc tttttgaacc ttaatcctac
     8461 acttacgacc atgctagtcg ttgtattagc agctgtgata ttggagtatc tgcgaacgat
     8521 ctaccgaacc tattcagaaa tctccattgc cattttaatg gctggtggtt tggcgctagc
     8581 gttagtattg atgaatttga gcggagggaa ttcagcaaca agtatccaat cctatctgtt
     8641 tggttcgatc gtgaccatta catgggagca agtcatcttt ttaggaatct tattcgttgc
     8701 catcggtata ttgttctttt tattcaaacg gccgatgtat gttctgactt ttgatgaaga
     8761 taccgcccac gtggatgggc tacccgttca tttgatgtcg atgatgttca atgtgattac
     8821 tggtgtggct attgctgtga tgatcccgat tgccggtgca cttttgatat ccgcaatcat
     8881 ggttcttccc gcagcaatcg gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat
     8941 cagtgtttgt gtaggatttc tagggatgct gtcaggacta actagctcgt tttatctaga
     9001 tacacctcca ggagcaacaa tcacattagt ctttattgct ttgttcctga ttgtaaatat
     9061 catcaaacga atattggttg ccttcagaag aaaaaataac taaattataa agaaaagaag
     9121 ccttataaaa acgaaaaatt ttttataggc ttctttttat tttaataaat accgaactat
     9181 tttcttattt tttcataaaa tgctcgaatt tttgaagaaa aacttatata atgtaaaacg
     9241 ataaaatttc ttaaaaggag ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg
     9301 acatgacaca gtatttattg aatcacccgc atgaattgat ttccttaaca agctttgccg
     9361 aacgttatga atctgctaaa tcttctatca gtgaagattt gggaatcatc aagaaaacat
     9421 ttaaagaacg agggtatgga accttggaaa caatcccggg tgctgcaggt ggtgtattgt
     9481 tcatccctga aatttcatat gaagaggcga aaaaatatat tgaatctcta gcagaacgtc
     9541 tatcagaaca agaccgtctg cttccaggag gatatgttta tctctctgat ttgttaggag
     9601 aacctgaact gctgcgtcaa gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa
     9661 tcgatgctgt aatgactgta gccacaaaag gcgtacctat tgcccaagct gtgtcctatt
     9721 atctgaatgc gccattcgtt attgtacgcc gggactcaaa aataacagaa ggatcaacag
     9781 taagtgtcaa ctatgtgtct ggatcttctg aaagaattga aaagatggaa ctgtctaaga
     9841 gaagtttgaa acgtggttca cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa
     9901 cagtcaacgg aatgaagagc ttgattgaag aattcgaatc agaacttgtt ggcatcactg
     9961 tatttgccga atcaaaattc aacggtcatc gcgccatcga tgattatacg tcactgcttt
    10021 atgtcaaaga tgtagatact caaacgaaaa atattacggt tgtgccagga aattattttt
    10081 cagaataaga ggttgtgaaa aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    10141 atggctttcc acattttttc ataaatttgg cttaataccg agaaactggc ttttgaacac
    10201 cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag tattagctca ataaacggaa
    10261 aaattgctcc tcatattttg ccgtttattg agctaatacc gaaggtcagc tgattgaaaa
    10321 acgtttaatg aagaggttgt aagcctaccg tttaactcta agtcacaaga aacgatttca
    10381 aaaaacagct tttcatgttt tatgagtagt gtgacttgtg accgaggagt tggcaggaga
    10441 gcaccggtta ccaaatagat aggcccgtta tggtctatct atttttttat ccagaaaacg
    10501 ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa
    10561 atcagcaaac tatatttagg agtgacgcag ttggacgcac gttatgcaat tattttggcg
    10621 gcagggaaag gtacccgcat gaagtcaaaa ttatataaag ttttacatcc cgtttctggt
    10681 aaacctatgg tagaacatat cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt
    10741 acgatcgttg gacacggagc agaacaagtt aaagcacaac taggagaacg cagtaaatat
    10801 gcgttacaag cagaacaatt gggaacagga catgctgttt tgcaagctgc ttctttcttg
    10861 gaaggaaaaa aaggaacaac gttagttatc agtggcgata caccattatt gactactgaa
    10921 acgctgaaca atctttttga atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg
    10981 gcacaagcag aaaacccaac aggatatgga cgaatcatcc gtgaccatat tgggatcgta
    11041 gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag ctcttgtaca agaaatcaat
    11101 acaggaactt actgttttga caacgaagct ttgttcgatg cgttaagcaa agttggaaca
    11161 aacaatgctc aaggtgaata ctatctaaca gatatcatcg aaatcttaaa agaagaaggc
    11221 catacagttg ctgcttatca aacggatgat tttgaagaat caatgggagt caatgatcgg
    11281 atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa ttaaccagat gcatatggtc
    11341 aatggcgtaa gctttgtcga ttcagcaacg acatacatcg atgcaggcgt agaaatcggt
    11401 ccagacacat tgatcgaagc aggtgtacaa atccaaggaa atacagtcat tggttctgat
    11461 tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt
    11521 gaaaactcag tcattgaaag cagccatgtc aagaaacatg cagatgtggg accttatgca
    11581 catttacgac caaaagctga aattggcgaa aacgtccata tcggaaactt tgtggaagtg
    11641 aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact tgacttatgt tggtgatgct
    11701 acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag
    11761 aacaaacatc atacgactgt cggcgaccac agctttattg gttctagcac gaatatcatc
    11821 ggacctgtcg aggtagctaa aaacagttcg attgctgccg ggtcaacaat tacagacaat
    11881 attccagaat atgcgctggc aattgcgaga gcaagacaag tcaataaaga aggatatgca
    11941 aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca cttgatttat ttaaaaaata
    12001 tgactactat ttattaggta gaattctaga gttgtgaaaa agaaagtgga ggtccccatg
    12061 tcaaaacatt actttgaccc aagattaaaa atttttgcgt taaattccaa tcgcccatta
    12121 gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa aatcatctgt tactcaattt
    12181 agtgatggag aaattcaagt aaacattgaa gaaagtattc gtggatcgca tgtctatgtt
    12241 attcaatcaa cgagcagccc agtcaatgat aacttgatgg agttgctgat catgattgac
    12301 gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca tgccttatta tggctatgcg
    12361 cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag cgaaattagt agcaaacatg
    12421 attcaaaaag ctggtgcaac aagaatgctg actttggacc ttcatgcagt ccaaatccaa
    12481 ggtttctttg atattccagt cgatcatttg atgggtgcgc cacttattgc caattatttc
    12541 ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt caccagacca tggtggtgtc
    12601 acacgggcac gtaaattagc ggaatttttg aaatctccga ttgcgatcat agacaaacgc
    12661 cgtccaaaag caaatgttgc agaagtaatg aatatcattg gacaagtcaa aggcaaaact
    12721 tgtgtactga tcgatgatat gatcgataca gcaggtacga tcacattggc tgccaatgct
    12781 ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta cacatccagt attatccggc
    12841 cctgctttac aaagaataga agattcagct atcgagcgtc tagttgttac agactcgatc
    12901 tatcttccag aagatcgtaa aatcgacaaa atcgatgaag taagtgttgg cggactaatg
    12961 ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa
    13021 aataaataat aaataaactc acaaagggga gtccaattgg gctgagattg aatctatttc
    13081 taaacccttc gtacctgtat cggttatgcg agcgtaggaa ttgtgaataa acagctgttt
    13141 gactattttc gaaagctgct tcctcctttg tgactttttt attcaaagga ggaagttttt
    13201 ttatgggaaa aaatagagtt tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt
    13261 tgtcgttgat tccgatccag atcggcagca gtttttctat ttcattaggg cagattccat
    13321 tgactctttt tgcgttaaga agagggacaa aggcagggat gctggcaggt ttcatttggg
    13381 gcatcctaca ttttccgttg ggacaagttt attatttaag tgtcgttcaa gtgttgattg
    13441 agtacccgat tgcctatact tttgcaggcg cagcagggct catggcttca aatgtccaaa
    13501 aaggctatgt agacgataat acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga
    13561 ttggcgcact ttccagatat ttttggcatt ttattgcagg agtggttttc tggggagcgt
    13621 atgcactatg ggggatgaat ccttggctct tttctttcgt aatgaatggt gccagcgggt
    13681 tgagtacagc tattgtaacg atcatcgttt tattagggat ccaacaaacg actccttcga
    13741 tttttgtgcc agataaacga caggagctgt aaataaaaaa gaagcaagaa acttgagaca
    13801 gaatcatgtc ctagtttctt gtttcctttt tattttttga taagaaatag atctacttca
    13861 atggcagata agaaagatcg gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt
    13921 agattaaagt agcgatgatt tcctcttctc gataccgggt gccttttaat ttccaaatat
    13981 aatcttcaat cacatagatt gggatacgat atttaccatc gatcaaacca acttgatcaa
    14041 aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc tttcaataat tccacactga
    14101 ttttttcaac aggtgtttct tttttgaaaa aagatttttt tacattgatc ttatctgaca
    14161 agatcgaact taatttacta gcaaaaatcc tataaaattc atctaaatag cgataataaa
    14221 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
    14281 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
    14341 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
    14401 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
    14461 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
    14521 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
    14581 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
    14641 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
    14701 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
    14761 tttgtgattt ttgtattttt ttatttaaag gaggttttat gaattgtttt cttatggaat
    14821 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
    14881 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
    14941 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
    15001 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
    15061 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
    15121 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
    15181 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
    15241 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
    15301 gcacttgttt taggtggagg aggagcgcac ggtgcttatc agattggtgt gtggcaggcg
    15361 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
    15421 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
    15481 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
    15541 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
    15601 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
    15661 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
    15721 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
    15781 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
    15841 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
    15901 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
    15961 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
    16021 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
    16081 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
    16141 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
    16201 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
    16261 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
    16321 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
    16381 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
    16441 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
    16501 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
    16561 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
    16621 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
    16681 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
    16741 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
    16801 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
    16861 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
    16921 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
    16981 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
    17041 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
    17101 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc
    17161 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
    17221 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
    17281 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
    17341 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
    17401 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
    17461 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
    17521 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
    17581 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
    17641 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
    17701 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
    17761 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
    17821 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
    17881 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
    17941 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
    18001 gctgaacaaa gaaatacaat aaataaacag aattggattt cttcgcttag cttctttgct
    18061 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
    18121 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
    18181 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
    18241 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
    18301 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
    18361 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
    18421 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
    18481 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
    18541 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
    18601 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
    18661 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
    18721 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
    18781 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
    18841 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
    18901 atgcagatga agtaatcgaa aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
    18961 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
    19021 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
    19081 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
    19141 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
    19201 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
    19261 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
    19321 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
    19381 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
    19441 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
    19501 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
    19561 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
    19621 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
    19681 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
    19741 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
    19801 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
    19861 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
    19921 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
    19981 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
    20041 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
    20101 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
    20161 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
    20221 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
    20281 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
    20341 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
    20401 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
    20461 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
    20521 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
    20581 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
    20641 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
    20701 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
    20761 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
    20821 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
    20881 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
    20941 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
    21001 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
    21061 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
    21121 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
    21181 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
    21241 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
    21301 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
    21361 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
    21421 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
    21481 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
    21541 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
    21601 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
    21661 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
    21721 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
    21781 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
    21841 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
    21901 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
    21961 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
    22021 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
    22081 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
    22141 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
    22201 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
    22261 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
    22321 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
    22381 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
    22441 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
    22501 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
    22561 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
    22621 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
    22681 cggaaacgag taattacgcc gacaccggta aaaaaagcgg ctctgaagca cggtatccgt
    22741 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
    22801 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
    22861 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
    22921 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
    22981 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
    23041 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
    23101 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
    23161 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
    23221 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
    23281 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
    23341 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
    23401 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
    23461 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
    23521 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
    23581 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
    23641 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
    23701 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc
    23761 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
    23821 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
    23881 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
    23941 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gacgaaacga ctggtggatc
    24001 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
    24061 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
    24121 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
    24181 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
    24241 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    24301 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    24361 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    24421 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    24481 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    24541 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    24601 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    24661 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    24721 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    24781 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    24841 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    24901 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    24961 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    25021 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    25081 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    25141 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    25201 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    25261 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    25321 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    25381 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    25441 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    25501 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    25561 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    25621 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    25681 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    25741 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    25801 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    25861 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    25921 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    25981 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    26041 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    26101 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    26161 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    26221 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    26281 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    26341 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    26401 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    26461 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    26521 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    26581 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    26641 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    26701 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    26761 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    26821 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    26881 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    26941 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    27001 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    27061 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    27121 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    27181 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    27241 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    27301 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    27361 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    27421 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    27481 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    27541 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    27601 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    27661 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt
    27721 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    27781 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    27841 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    27901 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    27961 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    28021 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    28081 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    28141 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    28201 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    28261 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    28321 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    28381 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    28441 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    28501 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    28561 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    28621 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    28681 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    28741 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    28801 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    28861 gatggccgat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    28921 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    28981 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    29041 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    29101 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    29161 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    29221 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    29281 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga
    29341 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    29401 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    29461 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    29521 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    29581 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    29641 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    29701 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    29761 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    29821 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    29881 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    29941 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    30001 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    30061 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    30121 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    30181 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    30241 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    30301 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    30361 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    30421 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    30481 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    30541 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    30601 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    30661 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    30721 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    30781 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    30841 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    30901 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    30961 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    31021 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    31081 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    31141 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    31201 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    31261 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    31321 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    31381 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    31441 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    31501 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    31561 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    31621 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    31681 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    31741 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    31801 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    31861 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    31921 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    31981 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    32041 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    32101 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    32161 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    32221 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    32281 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    32341 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    32401 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    32461 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    32521 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    32581 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    32641 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    32701 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    32761 gcgcctgaat cttcagcaac agaagaatca acaacagcgc ctgaatcttc agcaacagaa
    32821 gaatcaacaa cagtgcctga atcttcagca acagaagaat caacaacagt gcctgaatct
    32881 tcaacaacag aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt
    32941 gatacaggaa acggcacagg aagtagtact ccggctccaa cgccaacacc aacacctgaa
    33001 caaccaaaac ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc
    33061 gtagcagaag cttacaaata tattggtact ccatatgttt ggggcggtaa agatccaagt
    33121 ggctttgact gctcagggtt cacacgctat gtttacatgc aagtaactgg tcgtgacatc
    33181 ggtggttgga cagttcctca agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa
    33241 gctggagact tgttattctg ggggtcacaa ggcggaactt accacgtagc gattgcttta
    33301 ggcggaggac aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt
    33361 caatggtttg cccctgactt tgctgtcagc atgtaagaat ga
//
LOCUS       DADVXN010000017        32431 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.017, whole genome shotgun sequence.
ACCESSION   DADVXN010000017 DADVXN010000000
VERSION     DADVXN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.017"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            141..2750
                     /gene="clpB"
                     /locus_tag="KB942_000736"
     CDS             141..2750
                     /gene="clpB"
                     /locus_tag="KB942_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBB6851185.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2901..3587)
                     /locus_tag="KB942_000737"
     CDS             complement(2901..3587)
                     /locus_tag="KB942_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851186.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3565..4059)
                     /locus_tag="KB942_000738"
     CDS             complement(3565..4059)
                     /locus_tag="KB942_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6851187.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4079..5299)
                     /locus_tag="KB942_000739"
     CDS             complement(4079..5299)
                     /locus_tag="KB942_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851188.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5446..7281
                     /gene="lepA"
                     /locus_tag="KB942_000740"
     CDS             5446..7281
                     /gene="lepA"
                     /locus_tag="KB942_000740"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBB6851189.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7477..8388
                     /locus_tag="KB942_000741"
     CDS             7477..8388
                     /locus_tag="KB942_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851190.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9384..9635
                     /locus_tag="KB942_000742"
     CDS             9384..9635
                     /locus_tag="KB942_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851191.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9718..9990
                     /locus_tag="KB942_000743"
     CDS             9718..9990
                     /locus_tag="KB942_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851192.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            10080..10319
                     /locus_tag="KB942_000744"
     CDS             10080..10319
                     /locus_tag="KB942_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851193.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10466..14749
                     /locus_tag="KB942_000745"
     CDS             10466..14749
                     /locus_tag="KB942_000745"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBB6851194.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14848..15213
                     /locus_tag="KB942_000746"
     CDS             14848..15213
                     /locus_tag="KB942_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851195.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15215..15667
                     /locus_tag="KB942_000747"
     CDS             15215..15667
                     /locus_tag="KB942_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851196.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16281..17591
                     /locus_tag="KB942_000748"
     CDS             16281..17591
                     /locus_tag="KB942_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBB6851197.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17614..17925
                     /locus_tag="KB942_000749"
     CDS             17614..17925
                     /locus_tag="KB942_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851198.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18285..19541
                     /locus_tag="KB942_000750"
     CDS             18285..19541
                     /locus_tag="KB942_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6851199.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19538..20083
                     /locus_tag="KB942_000751"
     CDS             19538..20083
                     /locus_tag="KB942_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851200.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20084..20584
                     /locus_tag="KB942_000752"
     CDS             20084..20584
                     /locus_tag="KB942_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851201.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20665..21221
                     /locus_tag="KB942_000753"
                     /pseudo
     CDS             20665..21221
                     /locus_tag="KB942_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21241..21585
                     /locus_tag="KB942_000754"
     CDS             21241..21585
                     /locus_tag="KB942_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851202.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21604..22515
                     /locus_tag="KB942_000755"
     CDS             21604..22515
                     /locus_tag="KB942_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6851203.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22594..23586
                     /locus_tag="KB942_000756"
     CDS             22594..23586
                     /locus_tag="KB942_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6851204.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23583..23801
                     /locus_tag="KB942_000757"
     CDS             23583..23801
                     /locus_tag="KB942_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851205.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23814..24224
                     /locus_tag="KB942_000758"
     CDS             23814..24224
                     /locus_tag="KB942_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851206.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24276..26804
                     /locus_tag="KB942_000759"
     CDS             24276..26804
                     /locus_tag="KB942_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6851207.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26820..29030
                     /locus_tag="KB942_000760"
     CDS             26820..29030
                     /locus_tag="KB942_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851208.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29038..30129
                     /locus_tag="KB942_000761"
     CDS             29038..30129
                     /locus_tag="KB942_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6851209.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30150..30692
                     /locus_tag="KB942_000762"
     CDS             30150..30692
                     /locus_tag="KB942_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851210.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            30887..31144
                     /locus_tag="KB942_000763"
     CDS             30887..31144
                     /locus_tag="KB942_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851211.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31158..31490
                     /locus_tag="KB942_000764"
     CDS             31158..31490
                     /locus_tag="KB942_000764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851212.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31535..32083
                     /locus_tag="KB942_000765"
     CDS             31535..32083
                     /locus_tag="KB942_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6851213.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32090..>32431
                     /locus_tag="KB942_000766"
     CDS             32090..>32431
                     /locus_tag="KB942_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851214.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKR"
BASE COUNT        11099 a         5010 c         6886 g         9436 t
ORIGIN      
        1 tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat tttgaccaaa
       61 attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga tcttaccttt
      121 aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt caagaagcga
      181 ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac attgcccatg
      241 tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg gatgccggat
      301 tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat ccagttgttt
      361 ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga ttattgaatg
      421 aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa gtcgttattt
      481 tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa aacggattat
      541 ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga gtgacctcac
      601 aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta gtacagcaag
      661 tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt gatgtcatcc
      721 gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc ggtgtaggga
      781 aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg cctgaaaatt
      841 taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga gcaaaattcc
      901 gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa agtgatggac
      961 gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa acagaaggaa
     1021 gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta catttgatcg
     1081 gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg ttggaacgtc
     1141 gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt attctgcgtg
     1201 gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac gcattagtag
     1261 cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac aaagcaatcg
     1321 atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg ccaacggaat
     1381 tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca ctgaaaaaag
     1441 aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta gctgaacttc
     1501 gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa gtcaattctg
     1561 tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat gcagaaaata
     1621 actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa ttagaaaaag
     1681 aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt caagagtccg
     1741 ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc gtaacgaaac
     1801 ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat aaacgagtga
     1861 ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca cgggctggat
     1921 tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact ggtgtcggaa
     1981 aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat catatggtac
     2041 ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc ggagctcctc
     2101 caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga cgaaatcctt
     2161 atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc aatatcttgt
     2221 tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta gatttcaaaa
     2281 atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa ggcgtgactg
     2341 ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta cgtggaaact
     2401 tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca ttgagcctag
     2461 acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt ctggaacatc
     2521 aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa aatgcttatg
     2581 aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta gaaacaccac
     2641 tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg attactttac
     2701 ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa tcttccattg
     2761 agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata
     2821 aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc
     2881 ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag
     2941 taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc
     3001 gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg
     3061 ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc
     3121 cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac
     3181 caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca
     3241 agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata
     3301 ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac
     3361 aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa
     3421 atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat
     3481 ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa
     3541 gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt
     3601 tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc
     3661 tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat
     3721 ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat
     3781 gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc
     3841 tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat
     3901 tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat
     3961 cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc
     4021 acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc
     4081 aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg
     4141 tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca
     4201 tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta
     4261 agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag
     4321 cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt
     4381 gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga
     4441 tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc
     4501 agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat
     4561 caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag aacagaacac
     4621 ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga
     4681 aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg
     4741 caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac
     4801 cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag
     4861 acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga
     4921 tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc
     4981 catggttagc atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg
     5041 caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt
     5101 ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc
     5161 gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa
     5221 gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc
     5281 taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa
     5341 attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc
     5401 gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa
     5461 atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg
     5521 aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg
     5581 caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta
     5641 aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt
     5701 gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag
     5761 ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt
     5821 tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc
     5881 gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt
     5941 gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc
     6001 gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt
     6061 gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg
     6121 gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag
     6181 gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt
     6241 tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta
     6301 gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat
     6361 tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa
     6421 ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt
     6481 gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa
     6541 cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa
     6601 acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt
     6661 caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt
     6721 gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat
     6781 gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc
     6841 gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat
     6901 caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta
     6961 agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg
     7021 aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa
     7081 atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt
     7141 ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg
     7201 aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggat
     7261 gagcaagata gtgtgaaata acttataatc aatcgaaaaa cctattaaat aaaccttttt
     7321 gagtagatgc aacactgagg attatctgta taaaagattt taaactgtca tttggttatc
     7381 acggcgatac taaaattaac aaaattggaa tatttttctt gaagattttt agctgtcagt
     7441 atatgatgaa agcatctcat aggaggtgct tttttcatgg ttgataattc aagtggaagg
     7501 gttcttaaat ctctaagaaa agagaaaaaa ctatctcaaa aaaagttagc tgatttagca
     7561 ggtatttcac aaagtacttt agttaaatat gaaaaagggt caagaaaaat ccctaaagac
     7621 gtagataata ctctaagcaa gattctgaat atagaaactc taataaaaga tgaggaagat
     7681 aagattgaga ttctcatcgg taaactaatt gcatacagag atatgaataa gttacttaac
     7741 aaagagttag ctgataatat aggaattagt gaagtattac tcagttatgt gttaaataga
     7801 aagagaaatc cgtcaaaaga aatgcaaaaa aaaatcgata tttttctatt aagtaatgaa
     7861 aaagaaattt tgaaggagat taatcgtgat agtgagatat ttagtttatc aaaagacgat
     7921 aaaattgtta tgggaaaaag aatacgagaa gtccgaaaga accgggaaga gacgcttgaa
     7981 aaatttggta aaaactttac catctataca ggcaaaaatg ttataagtag atgggaaaaa
     8041 ggtattaata ttccagatat tgaaaaacta atgaatattg cctatcttgg taaggtcact
     8101 gttccttacc ttatgtacgg agaagattat aagaatatac ttccaaaaga tgaaagagtt
     8161 agcgatttta aaaagataaa ttcttttagt atgggattaa ggatgaggaa aattaggaaa
     8221 gattattatc tagaaaggga ggagtttgga aagctttttt ctccttcaat ttcaaaatgg
     8281 tccatagata ggtatgagaa tggtagagat atacccaata ctaatcgaat tatccaatat
     8341 gcttatatag gaaatctttc tttagaattt ttgatttacg ggatataaaa atagtttacg
     8401 tcgatactta ttaagttatc gacgtttttt attatagaaa aaatttataa aaggaggttt
     8461 cttgatatga taagaaacaa aaagggtttg ttgaaagttg ctttactggc agcttttttc
     8521 taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaactaa
     8581 ctgttatgaa taacagctag ataaaatggg ctatgtatgt tatccaacaa tttagtaatc
     8641 actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt
     8701 tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg
     8761 gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt taatatgtca
     8821 gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa
     8881 atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt
     8941 aatcggataa tgcgagattc gtacggtctt ttttaaattt ctctaaaaaa aacggcatcg
     9001 aatgggttgg gtgattgata agcaatgaat catagactga tatagaataa aaaatgattc
     9061 tatttatctt tttacatcta tcgttttttc aagtagccgc aaattatatc agcattcaac
     9121 aatttttgta tacaagttct agtaaaagga tctgaagaaa aagttggttg ttgtgaagat
     9181 aaaaaaagaa tcaggtttta ctgagttttt cttcaaaatt tgtgttatta ggttaggaca
     9241 aagataagaa gaagtatgaa gatttaaaaa aggaatgagg aaaagatagt aatttccgat
     9301 tattggagtt tttttattta taaagaagaa gcgaataagt tgtgattcct agatttcgcg
     9361 cgtgattaat taatgagcaa aaaatgcgga aagttttagt gtttaataca cgaggacaaa
     9421 gtttcttagg tattgaaggc acacgtgtaa cggttgatgg gtggtgtgat tgtttcgagg
     9481 ttggaaaaca tgttgaactt atggcattta ctgagctaaa agataagaac aacgtggaat
     9541 ttttcgagag tgatattggt tgggacgatc atgaagaagt ttatggaaaa tttattttgc
     9601 ggaagggaaa ttcttatacg aatggggaag cataagtagc gatttgttag agatcattga
     9661 taggattgaa attgtcggga atatttatga gaatctagag ttatttgagg tgatagaatg
     9721 agtgatggtt ttgaacaagt atcaataatt aaaaatcaag ttcgggagat tcttagaaaa
     9781 aaaggctatt tggtagatag ttattttgaa ggagattacg aaacatgggt aggtgtctat
     9841 gctcgtccag agaataaacc tacctattta gatcctacga cgtcagaaga tgcctattta
     9901 caaaataggt atcgagtaga tggttttaag caagactttg cggagtggtt tgaatgggaa
     9961 attgaaaacg gagaagtaaa agaagaataa gagtacttgt tgtatcaaaa taagccagta
    10021 gaacaaccaa accagtagct agtaatgaca ataatactga ggaatcggat cattatggta
    10081 tgattgatat ttctcttcca gaagatctta agccagatcc tgtaacaatt ggcaatagtg
    10141 tcatgatttt tgattcatta gatgaagcta tgtcatatgg ttggaaagaa attaaggatg
    10201 aaggtagtaa ttggtatggc atgagatttg gtgatggtga agttgccaaa aaagctggta
    10261 ttccaattgg gaaatggaca gttgatttcc acgaagcagg ttcttttttt gttctgtaat
    10321 tatagtggga gggtaaaatc attgatgaaa tgtgttcaga taagagcgcc agataaaaat
    10381 agaagagaca gttcacgtcg ttatcacttt tggtaacggc gttttgtttt attatataaa
    10441 attctcagga aaaggaggaa ttcttgtgta tgcaaaaacg aaaatgaatt ggttgaaagc
    10501 actcgcattg gtggcttttt ctcaattctg tttcttggtg ggaactgctc aagcacatgc
    10561 agaagaaacg acggctatac aacctacgat ggaagccatt gcccaagtcg cttctgaagt
    10621 gacagaagtc caaccagcag aagccggggt cccagctggt gaagccactg ttgaacaggc
    10681 ccctgtgaca gaagcgccaa cggtcaattc caaaacgact ctcgatcaga cgattgggga
    10741 aacacaacac ccagggaata gtcctggtac ttcacaacca gttgaagaaa ctgcctcaat
    10801 tacaacccct gcttcagaaa cgtccactct agaaggagaa aaagctgccg aagcaacgcc
    10861 ttctgtgcag ccagagtctt ctgagaaaca agcggaagaa aagaaacgaa tagaagaaac
    10921 agtggaacaa ccagcggcta ctgctgacaa agaaggaaaa aatgaggaaa acgttgaaaa
    10981 aacaatctct tcaaccgcag ctagtcaaaa tcaaccaacg aaaccaaaca aagaatctac
    11041 ggctagtatt gaaaatcaaa agaaaaaaga gcagaacatt ccaaaaacag gtacacctac
    11101 actgttagta acagtaccaa ccaatcgttc ggttcgtgtt cgccgtgcaa ccaatgcagc
    11161 tgttttaggt ggtcggaaaa ttgattgggg cgaaaatatt ggtgttactt ctagtccgaa
    11221 caatctaaac ttcactttaa attatatggt tcgttttgag attaatggac aagtggcgtt
    11281 ttgtattgag cctggtgtct cagccgcaga aggtcaagtt tatacagcta ctgctttgaa
    11341 aaactatttg aaaaattcaa atgtacggaa aaaaacaagc ttgatctctt atttaggata
    11401 tgtcacaaat aaagacaaat ccgatgaaca atacattgcg gcacaattta tgatttggga
    11461 agctttgggc acaaaaatcc caatcaaaaa tatccgtgtt gattatgatc gtcgaaaagc
    11521 agaaattcaa cgtttgatta ataactttgg tgaaaaagca acttttaata atcaaacaca
    11581 tacggtaaaa attggcgaaa cgttgaaagt aaagaataaa aatgattttt tgaaatatgt
    11641 gaaagacatt caaaaaccaa aaggtgtcaa tgtatctatt cacggcaatg aagtatggat
    11701 tacagtcaca aaagaggcac cggataaagc aaaaattcaa ttgaataaaa ttaaagttca
    11761 aggtgcgcca ttagcctatc gtcgtccagg agctcaaaca attggtgtgt tacatccaac
    11821 agaacctaat gcgtctgtta ttaatttaca aattctaaaa caaggaaaca ttcgtgtgtt
    11881 taaagaagat gcggaaactg gtgtgaaagc acaaggtgca gcttctttag aaaatgcggt
    11941 ctatggtttg tatcaagcag atgggaaaaa gattaaagaa attaccttga aaaaaattaa
    12001 cggcaaagta caggcagaaa ttaaagatct tgatcctggc aaatatgttt taaaagaaat
    12061 caaagcgcca aaaggttatg tgctttctga tgaagcaatt gaaattgttg ttgaacctgg
    12121 aaaaactgtc aatgcgaccg caaaagatca agtgatcaaa ggaaccattg atttagtgaa
    12181 agttgctaat aaagatttag tcgattcaac gaatccagat aacaaaccaa aattagctgg
    12241 cattgaaatc actttgacgt caaaaacaac tggcaaagtg gtgaaaaccg ttgtgacaga
    12301 caatgatggt tatgctactt ttggtaaaaa taccgttgtc tttgatacgt acattctttc
    12361 tgaaaccaaa ggaaaagaag ggtataaact atttgaacca cttgaagtca caatttctga
    12421 aaatgggcaa actttccatt atgttttaga agataaagta attgaacaac gtcttaaagt
    12481 ggtgaaagtg gatcaagaaa caggaaaagt gattccactt gctaatacag aattcaagat
    12541 ttttgatact ttagccaatc gttatgtcac aatggagatt ccaaatgata cagaaacaac
    12601 ggatatcttt aaaaccaatg aaaaaggctt tttcctaact aacggtacac ttacttatgg
    12661 aaaagatcgc tatcgtttgg aagaaatcaa agcccccgta aattacgttt tgaatcaaaa
    12721 cccattagtg ttctcgatta ccaatgatac agaaccgatc aaagtgatcc attttgcgaa
    12781 ccgtcaagca cggaaaagtg taaaattatt caagtatgaa gaatggaaca agaagaaaac
    12841 gccgctcgca ggtgtgaact ttaccttgta taacggtaaa aatcaagcaa ttggtgaata
    12901 taccactgat caaaacgggg aaattttagt gaaaggtcta gttgccgatg actattactt
    12961 cttggaaaat gccccattag aaagtttcca accaaataca gataaacaag cattcacggt
    13021 tgcattcaaa caaaatgcgc cagatgatgg ggaaacgtta gtggtttctg tacgtaatta
    13081 cttgattcca ccaaaactag aaacacaagc gtcaaataaa gccgatggtg aaaaagtggt
    13141 tgatccatta gaaacgatcg tcattaaaga cagtgtttct tataccaact tgtttgtcgg
    13201 aaaagaatac accatttcag gagtattgat gaacaaggca acgaatgcgc caattttaca
    13261 agatggcaaa gaaatcacaa gtaccttggc ctttattgca gacaaagtca atggcacaaa
    13321 agaccttgaa tttgtggtta atgctagtgt tctgaaaggt caaacgatcg ttgtttttga
    13381 aaaacttttc cgtgatggca ttgaagttgc agcacatgcg gatatcaacg atgaagccca
    13441 aagtgtgaaa gtgaataatc caaccgttca taccacggct gtctttgaag acggattgac
    13501 agtcgcagac ccaacaggat tgtttacctt aaacgatacc gttgaaatta ccgagattct
    13561 tccaggaaaa gcatacttgg tcaaagggat cttaatggat aaagcaaccg gtgaaccctt
    13621 gcttgtgcat ggaaatacgg tagaaacgag tgttggcttt attgccaaag cagaaaatga
    13681 aacggtgatc gtaccattta cctttgattt gacaggtata aatggtcgtg aaatcgtcgt
    13741 gtttgaaagt ctttttagaa atgatgaaga actagcaaac cataaagaca tcaacgacaa
    13801 agggcaaacg gtacgagtaa ctaacccaag tgtacgtaca acggctacac ataaacccac
    13861 tggcttgaaa gaggttaatc cattaacgaa agtcaccatt acagacgctg tgaaatacca
    13921 cgatttggtc gttggaaaaa catacacagt caacggtgtt ctaatggaca aagtaacgga
    13981 aaaaccagtg ctagtagatg gaaaagaagt cacaagtacc ttaacgtttg tggcagaaac
    14041 aacggatggt acagtcaatc tagactttat tctagacgca cgtgcgttgc gaggcaaaga
    14101 agtcgtggtc tttgaagatc tgtttagaga aggcgtatta ttggcttctc acgcagatat
    14161 taccgacaat gaccaaacca tcaaaatcag taatccaacc attacgacga aagcgacgaa
    14221 taaaaatggc ggaaaagaga tcttggcctt gccaaatcaa actgtgcttg aatgggtcaa
    14281 agtagacggt cttgcgattg gtcaaatcta tcgcttaatt gttcaaggct acttaacacc
    14341 agacggcaca ccatttgaag gcactcatgc ggaaaaagtc tttgcggccg ataaagaaat
    14401 gatggaattc ttatttgaat tcttagtgga cggtcgcaat cttgcaggaa aaggcttgag
    14461 ttttgcggaa tggctccaag cgaaaacgga agatgagaaa gaaacgtttg aagaagtggc
    14521 gaaacacaat gtagatctaa caaacaaaga tcaaacggtt caaattgtgg aagcgccaaa
    14581 accaccgcaa ccagtgaaac cagaacaacg ggttaaacca gctactgttg taccaatgtt
    14641 gccaaaaaca ggtgaacaaa caagtagtat acttatggtt cttggattag tagtattagt
    14701 cattgcttta ggtataacac tttataaagt acgtaaagcc aaagattagt catgaggaac
    14761 aagcggttag gatttttaac cgcttgtttg attgtaatgt agaaatattt agaatttatt
    14821 ttggttttta agaggaggaa aaactttatg gcagaaaaag cattgcgtat gaagaaagag
    14881 tttttaggtc aagtattttt attgggttca cgggaaccta atccaccaaa ggaaattaat
    14941 ggtgtgtctg ggcgatatgt tcaattacaa gcagagtgtt cgcgagaaga acagcctgct
    15001 ttttttgttt atctcacagc tgaagaggat aaaagattac gattaccaga aaaagaggtg
    15061 cgtatttcat ttgaagaagc ttattttgtt tttcgtaaag aacgagtgaa tagtcgtgaa
    15121 acaaatgata tgggacaaac agagcgtcga acaactggct atcaagatgt tggtgtcttt
    15181 ttaactggtg taaaaattgg ggaggttttg taagatggga ttaaaatttc caaataatta
    15241 tgacattaca gaagcattag atgaaacttg tttaggagcc gtacgctttg gcgcattaga
    15301 agagtctgat cgtgtatttg ataatgaaac aggcgattat acgggagaaa ttcgatcaca
    15361 gcgagcacaa ttagtttttg gaaatccaga tgaaccaaag attgcacgac gagttaaatt
    15421 tccagcagat gtagatttga ctggttttaa acgacgtgat cctgtgaaat taattgatcc
    15481 agaatatcat gtggtatata ttccagcaca agatggcaat cgtgaatatt cagatttagt
    15541 gatttatgcg aaaggattag agaaagtaga agctccaaca aataatccta atatgaaaca
    15601 atcacaagaa aaaggtttag atcaaaaaca accaagtgtt gattctaagg aaaaagagaa
    15661 aaaataaggt ttttttgaaa gaataggaag gagtcttcct attcttttta tctaagatag
    15721 aaggaagggg agattttgaa ttcagataaa accaatcaaa tactttgaga gattgtgctt
    15781 gttatcaaaa tatccaacaa ttgattgagt attttggaga caatccaacg atttttgtac
    15841 aaacaaatgc atatatttgt gcggttattg ggcagtgtat gtttttacgt gatcttgttc
    15901 gtttttcatt accgtaccag tggaaacaag atcatcagga agtgatttgg attcgtatag
    15961 tacgaatatg taatatgtgt gcacaccctt ataaaaatcg attaaatctt tttcgattat
    16021 gtttttttac taaatgtgat attcctgtat tagttgcttt ttgtcaaaag tttttagcac
    16081 aggaaaagta aaaacagaaa ggaggaatgt gacaattgaa agctcatttt ttcaagtatc
    16141 gtggtcggcg aattcgctat ttcaatcgtt atttatggtt ttgctattat ggtttctttt
    16201 tgtttccatt tttgattctg ttaggttggt ttagttggaa agtattttat ccacgtttaa
    16261 gtacgttttc gaattggcaa atgtatctta ttcctggaat aagtattgtg ttgtatttgt
    16321 tgctggttag tgggttaatt ttagggttga tgcattggtc gcgactaaaa gaaggatatt
    16381 ttgcctctgt ttattggcgt cagttacttg ttcgaatgtt aattgacaat cggtttgttt
    16441 atacaaagaa acaagttagt gagaaacaaa caagggaaaa aatgagatta ccaaaagttt
    16501 atttttatca aaagaaacaa gaattagtgg tttcttttcc tttagatggt gggcgttttc
    16561 acgaacgatt tttaaaaatg ggtcatttgt tatctgaagt atttttgcgt gatttaattc
    16621 gtgaagaacg agaaaaagcc cgaatcaatt atgtattcct ttcagattct ggtcggattc
    16681 ctattgatca atgtatagca gaaaatggac gtattcattt aatgaaaact ttggattggg
    16741 tatatgatca atcaccaaat atgttgattg ctggtgcaat cggtggtggg aaaacctatt
    16801 tattgtattc tttaattcaa gcttgtttaa gtgttgggac agtagatatt tgcgatggaa
    16861 aagcggcaga tttagcagca ttaggtgacg tcgatgtatt caaaggacat gtgttttaca
    16921 aaataaatga agaaatgatt atttgtttac gaaatgcatt aaaagaaatg aatcaacggt
    16981 atgcctatat gaaaaccatg aaagatccta aatatgaacc agggaaaaat tatgcttatt
    17041 atgggttacc accgcatttt atcttttttg atgaatgggc agctttttat ggttcgttgg
    17101 atatggaagc gagaaataga gtcgataaat tgattcagcc attagtatta aagggtcgtc
    17161 aagcagggat gtacattatt ttagcaatgc aacgaccaga tgcagaatat tttccaagtg
    17221 gtgttcggga taatttaaca ttccgtattt ctgttggtcg attgtctccc attggttatt
    17281 taatgacttt tggagaagat tttaaaaata agccatttta caacaaagat caacctggtc
    17341 gtggatatgc ttttccagga tttgatgtac ctgtagagtt ttttgcgcca tttgtttaca
    17401 aagattatca ctttatcaaa gaatttgaga aatttactga aatgattgtc tatgatttta
    17461 cagtaaaaga ggatagtcaa gatcaaacga tgggagatga taatcagttg ttacaagaac
    17521 aactagagcg ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt tctgatgcgc
    17581 atattttgta atcaactaag gaggtgaagt taaatggcag agagttgtta ttatagagta
    17641 gctacagcaa tacgaatgat taatccttct ctttcttcca gaacgtttta tgattggttg
    17701 aatcgtattg aacaagtaac tgattaccgc tttttgcgca aagaacgtgt ttttacagga
    17761 aaagttatta atcaagtatt gctaacgaaa aaagatatag aacgattgac tcgattgtat
    17821 cattatcgag tagatttaga agaggattta actttgtcaa tttatcgcgt tttttctcct
    17881 gaaaaatata gtgaaataac aaaattagat catttaattt tgtagttatt atctatcggg
    17941 agggctctag acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt aaggctgcga
    18001 agcatgggcc tataatgcgc actcgctagc agcgtgtgca gctcacaaag aggtcgtgcg
    18061 cttgcgcacg gcctctttgt gtaaagcccc ccgatatata atatgggggg cagaaataac
    18121 ataacacata gaaaaaagtt ttataaaccc ttttgtatca agtgattatc taaggtcagc
    18181 agttgtacta cttttcccaa aaaacgaggg ggactagttg atatatcggt gtttctaaag
    18241 ggttaaaaaa atcgattttg ttattttata aaaggagcga gaaaatgaca ccgattgagc
    18301 taaaaagcta cagaaagaag ttaagtgcaa ttcataatat aagtggagat aaaaagccgc
    18361 tatcacaagc aagatttgcg caattgatgg gctgctcaaa agattacatt agaaagatgg
    18421 aacaagggaa aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat
    18481 tgtatatgaa aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt
    18541 ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg
    18601 aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg
    18661 tgttctggca ttcaaaacga actgtaacag aaaatgtact tgtgcaattt tcaggacaag
    18721 gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat
    18781 attccatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagcc
    18841 gattcgatat tgcgttagat gaactttggg gattggaaac ggaagaacat ttcgatctct
    18901 ttgaattact agaaaaacaa aaagcaggtt tattagaaat ggactttaag agattccaga
    18961 atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta tctttgtatt
    19021 ttggatcgcg aaagagtcct ctctttttta atttttatga gaaacgtttt gaaattgcga
    19081 accgagaaaa aatttcagaa ttagaagcat taacgaaata tggtatttat aatcgatatg
    19141 agttacggtt agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt
    19201 caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaaactt attgtttggg
    19261 atagactgga aagtgatgca cggattgttg ataaacgatg ggcaaaatta tttcgttcga
    19321 tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct
    19381 ggtttttgaa acaagtagct ccgacattaa agttgttaca agaagcagat cgtattttag
    19441 gaagagactt tgtacaagaa gggattgata atgccgaact aaatgacgag caacaaaact
    19501 atctagaatg gatgggaacg tcaggaggga aaattgcatg aatgcatata cgacaacaga
    19561 atggctgcgc ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa agatggttga
    19621 tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta
    19681 tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga
    19741 acaagaactc gaagagttgg aatttagccg aaggaaagga aggctagaat tcattcgctt
    19801 ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat
    19861 gaagatatat gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat
    19921 ttcattggtg actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct
    19981 ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac
    20041 ctattgtgga tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat
    20101 ttatgttggt gcgtattggt cacgtgatcc aacagtatta gaaaatccag aggcggtaca
    20161 ctattgcttg cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcgtca
    20221 gttaccatat actgacagac gtttagatga acttcttcta aggtatccag caatgtataa
    20281 acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacatg aacttgaagg
    20341 gcgatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga
    20401 aatagaaaat gatcgctact attttatcaa ttattgtaag aagcgtaagt atcaagtaac
    20461 tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc
    20521 aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc
    20581 gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat
    20641 tttttttagg aaggagaaga tggtatgtca ctggaatttt taggagagcc aattgaaaag
    20701 gaagagttcg tttcgcatta tatggatttg tttattcagg tgataaaaaa ctttgtgcga
    20761 ttgatgaatt ttatccacgc gaacaagact atctagccgc tgaacgaata aaaatcagtt
    20821 tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag
    20881 attgtttaac gaataatgtg ctttctaaag aatacctatt tcacaaacgt gagaaaaatc
    20941 aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat
    21001 taacagtaga acgatatggt gcagagtttg gttatgtctt gcaagctgag cgtcatagag
    21061 ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc
    21121 gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc
    21181 tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt
    21241 atgtggcgat tattattttt ggtggtatgt ggtatggcat tgttctatgt gtatcgtgtc
    21301 tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga
    21361 aaaggattga agcggtatcg ttatgaatta cttgttgaat tttcggatgg gcgacaagca
    21421 cagtattatt tttttgcaaa ctatccaaaa tacaacacca gtgaactatt gattcaatta
    21481 ttcaaaaata atccatatgt tgggttagaa gaaaacggac gggagttctt ttatcgttca
    21541 gataatatta gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat tggaggaata
    21601 atcatgaaaa aaacaacatt agcagtcgta acaacactag gaatttcatt attttcgttt
    21661 caattaggga gttcattggt ttatgcagat gaaactgtag aaacacctaa gtcagaaatt
    21721 gtagaaacat tgccagggga aggattacca gatgttgaga cagagctaaa tggaggaaca
    21781 acggaaaaac cagcagaacc tgaacaattg cagtcagaga aacctgaaaa agagccagtg
    21841 acaccagaac cagagtttcc tgaatcaact ttaccgactg attcgatcga agaagaaggt
    21901 gtgcatagtt caacaacaac ggattcaagt atagtcacaa ctccggaaca atcaggaaca
    21961 accagttctt ctgaaaagaa tactgattcg tcaagtacgg aacagccaac gatgtcgtct
    22021 accagtgaag aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga agcaattcca
    22081 aaagaaaata aacctattgt tccagcaaaa gaagtcacag tatcggtaac cccaagcggt
    22141 gaaattacga cgaatacttc tcaaggaatg agtgtaccga ttgtcactag taatgtagag
    22201 gaattgaccc atattccaac accaaccacc ccattaaaag tagcaggtga tcaaacaatt
    22261 gttggtgtaa aagatggtat tccattaatc caagatagtg aagggaattt ggtaaaggat
    22321 ttgtcgattc ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac agcagatgga
    22381 aaaacaaaag tcttaccaaa gactggtgaa gaaatacatg ttgcactttc tgttctagga
    22441 ggagtgttga caagtattgc tggatttgtt ggatataaaa gaaagagaaa aggaatagaa
    22501 aatgagggta attgaatgga ctatttttca gaaaaacaag cttatcgttg tcaatcgtac
    22561 gtactagtaa aaacatacta gaaggagtag cttatgaaaa aacgacaact acggcggaaa
    22621 gaaaaagttt atacagagaa agtaccgaag caaaagaaaa tccattcaaa aaaagcagtg
    22681 aaatggattt ggtgtttggt tttatttctt tgtttttcag ggggcttggc attttttcaa
    22741 tccatgaaaa cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca attacaaaag
    22801 aaagaagtac aaagtcggtc agaattaacg tacacacctg aaattgaatc ctttatgaat
    22861 cgttttgttg ctttgtatat gaatacattc gataatcatg aacaagaaca acaacgtcaa
    22921 gaaacattaa aaaaagagtt ttatgcacaa aactttattc aagaaccagg aagtgtttca
    22981 ggtgttcgaa aattaactgc tgcttcgttt attggattaa aagaaattga aaccattcca
    23041 actgctagtt ataaagtaac ttatgatatt cgctcgatga atccagagag taagaaagaa
    23101 acaagtgtga caatcacaca agtactcaat cttccctttt ctatttcaaa caagggctgt
    23161 cgtattattg cttatcctta ttttacggca ctaccaaaga atacagaaaa acaacgaatg
    23221 attacttatg atgacacatt atacgaaacg gttgatgcgt cggaagcgaa agatgttgaa
    23281 ggatttgtgg aagatttctt agataagtat gcgaaaaaca atgcaaaaga tatgtcatac
    23341 atgatgaaag aacctgaagg actcaatcaa acagctaaag ttgatcaagt agagatgaaa
    23401 gtattgcgtt ataaagaaac gaaaaaacct tttattgtaa aagcaacggt cgtctttcga
    23461 gatggtgata cgtcactttt acaaaaagaa caaatgacga ttgtcattac gaaaaaaaca
    23521 aatcagtatt atgttgaaaa attaacacat aattggttgg ataggaaagg agaagaacgg
    23581 aaatgacact gaagggaatt gtagatttta tattaacgca aggtggctat tttttaataa
    23641 tggtggttgt ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga aatgcattaa
    23701 tatctatcgc agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca cgtgtatttg
    23761 atgcgtttgg taaaatcatt gaaaaattat ttatggatta ggaggatagt gaagtggatt
    23821 gggaacctta tgattattcg attgtttatg cggaacctta tcgcgtacaa cagattacaa
    23881 gcaattggcg tcttccattt ccagttgcat tagtcaatgc agcaactttt ttgttggtcg
    23941 taatctttga atatttattt ttacgttcat ttattggttg ggtcatttct ttttttccac
    24001 cagcaggagt tgttttttat ataggactcc cctatttatg tgtgttagca ctggcaaaac
    24061 taaaacctga tggaaaaaaa gtgcattttt atttatggga ttggttttgt tatatgattc
    24121 gtatgatgaa tggtgattgt gtgtattata actgggacaa ggtatcaaac aatgcattgg
    24181 aagaattact ttttgtgaat aagtatgttt atttagagga ttagaaaaag ggacaatcaa
    24241 ctggttgttc cttttttatt gggaggtgaa attcattggt gcttgtaaaa gaaccagtaa
    24301 aagcattcaa gaaaaattgt ttgttaaatg gagaggattg tatctgggcc tactatcatg
    24361 tttttccacg aacaatttca aaaaacacac caaaaaagac acaggaaata aaagaaatga
    24421 tggctcgttt actgttgctt ttagcagatt atgaagatgt ggagttggtg atgtatccac
    24481 gaacaatgaa tttacggacg aagtttgaca cattaggaaa gggattttct cccgatagtc
    24541 aagcaattgg tgcgtattat gcaaaagaaa cagtgacatt gttggaacga gaaggtcaag
    24601 tctatcgtcc ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg gtggagagtt
    24661 ttcatgatgt aataaatcta atctggttag atttagcagg aagtttagca cgcttatttg
    24721 gattagaagt agatgatttg gaaactttgt tttctgaaag tgatccttat gtaaaagaag
    24781 tgacacaatt actttatagc ttttctattc gtcccttagt aaaaaaagag ctgttgtatc
    24841 tcaatcgtct gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa aataattggg
    24901 aagtactgaa agacaatcaa ggaacgtatg tacgtatacc gaaggaacat aaaggccttc
    24961 ttcaattaga agatgagtta gggagtagtt atttagcatt gttgcctatt ggggaaacac
    25021 ctgaaaatat gagaaattgt gatctatttc aatttgctca ggatctttct ttcccagcag
    25081 aattgcgaat gaaaattcac tatccacgct tgaaaggaca gggaggtatt ggtttaaaac
    25141 tgaattggct atcggaacgg ttcaaaacag agcataagga attggttcaa aatgatgaag
    25201 aacctagtga acgcttatta actgttcgag gattagtaaa acatttaaag caacgagtag
    25261 ataatgggca acctgtcgtc gattggatcg catgttttat tgtatacggt caaacggttg
    25321 aagaagttcg tcaaaatagt gcaacggtta ttcaacaact tcatacgatt atccatatcc
    25381 accgtgcaaa aaatgatcaa cgagaattgt tctataaatg tttattaggg caaccattgg
    25441 gacggcagtt acattggaag caccgaacaa ctgtagaagc agttggcgaa tggttgtttt
    25501 cagcagtatc agaattaggt atggaatccg gctggtatat tggtcggcaa gatacattag
    25561 gggatggtgg ttctcgagga gaaaaaacac cattagaaga attaatttat gcgtcaaatc
    25621 gttttgtctt tttgaatctt ctagccattg cggaaggaat caaaggtgcg ttgtttgatg
    25681 cgcctcatgt agcagtaact ggaaaaaccg gtaaagggaa gaccttttta gttggcttgc
    25741 tctttttgta tagttcgttt atgaatgtac aaagtttatt tgtggatccc aaaggagaaa
    25801 agcgcaaatg gtttacgaaa ctcattcaag acccgtatta tcagaaacat taccctttgt
    25861 ttgtagaaca tttgcaaaaa atgagttatg tcacgttaga tgctacaaat ccccgtaatt
    25921 atggtgtatt agatccaatt gtttgtttat cccgagtaga tgcgaaacaa gtagcccagg
    25981 atatgattga tgagttaagt ccgttaggta ccaatcacct tttaaaaggg gcggtattaa
    26041 agggaatcca agaagtttta gttcaaaaag aagccggaca acaagttggc ttgatgcatg
    26101 tggtcgacta tttagaacaa atggaacaaa aagaggttcg agaatatggt gagtttttac
    26161 gcttgacggt agaagattcg attttacgtt taggattttc ttatggagaa aacccgggat
    26221 tagactttaa tgccaaaaca acgattttag agatccaaga tttgaaatta ccaaatgaca
    26281 atgtccgacc agaactatat acagatgcgg atcgaaagtc tttgtgtttg atgattagtt
    26341 taggtcgttt ttgtgaaatg tttggaaaac gagatagtag taagaaaaca gcgatttatt
    26401 ttacagaagc atgggtattt aataattcga acgcaggacg tagtattatt gcagcaatgg
    26461 cacgtgtggg gcgttcccag atgaatcaat tagtgttaga tacacaattt attggtgact
    26521 taggttcaga taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat gaagacagtg
    26581 agcgagaaaa gattttgaat catttacgtt tggaagtaaa tgaagcgaac ttagctgaaa
    26641 tgagccacat gattaaagga cagtgttgga tgttagatcc ctatggacgt gtaggaaaac
    26701 tcaatgtaca cagtttgttt gaagaaatga ccgctgcatt acaaacaacg gaaaaaacaa
    26761 gtcactctca agtagaagaa caggatacgt taatcatgca atagaaatga ggtgaaacaa
    26821 tggcttatat aaagcataag gaaaagaaat ggtgcgtttt gtttttttta acgattggat
    26881 tcggactgct ttttgttggt ggattggtgt atgcggcaga aaagcaggtg gatcaatcga
    26941 tctatgagat tgggaaattt catattgata ccttttcttc ttatttatct gatagtgaat
    27001 tttcattagg caaaatgtta tttggggcaa atggcacaga ttttctaaaa atgatgacga
    27061 atatgttctt tagtttaagt aaattgattt ggcaaggcct tgattttgtc attgagaagc
    27121 tctatgaagg agctgcaatg aacgagttaa ttcaccagtt tttcactttt tcggatacta
    27181 tttattcaaa aatatacgat cctattggtg cggggattgt cgtcttgtat gtgttatatc
    27241 tatttactct tcgtatattt agagggaaac attacgcaaa aatggtgttt attcgatttc
    27301 tcttcgttgt cattttttca atggtttggt ttggctatgg tagtaacgta cctagtaaag
    27361 gagaagcttt taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc tttatttttt
    27421 ctgctacaaa tagtctagat gaattggcga caaaagatgt caatggtgtt ttagtgccaa
    27481 cgacacaaaa ggaagccatt caaaaagtgc gccaacttta ttataaagcg gctgttgttg
    27541 atccgtatat attgttaaat tatgggacaa caaatttaga agaattgtat caagcagaga
    27601 ttgaaccaca agaatttcta gccaaggaag cgtcaagtga agaattatct gatattgaga
    27661 caaaagtgac cactgctgca ggagaagata gagggaaaaa gaaggcttat cgtgtctata
    27721 ttcaaccaac taaagctgtt tataaagcag tcgttggttc tatggtgcca ttgcttaatc
    27781 taagtattgg attaccactt gtagtgattg gcatgattcg ttttatcttt caaatgttgg
    27841 tgttgtgttt attacttgtg attccttttt cattactcgc ttcttttgtt ccagtatttg
    27901 attatatgct catgaatacc ttaaagtcct ttttgggctt gatgtttcaa aaatcttttt
    27961 acagtgtgat tattttggtg gcatttttga tctataacat agtagacgat ttaattccaa
    28021 tgaatactgt ttttggtttt ttaggaaata tgtttgtcaa agcactccta tcaattttac
    28081 tcattgtaaa acggaaagat ttattaaaga aactagggtt aggagctgca gatcaaacta
    28141 taaacaacac gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa ctgcctggtc
    28201 aactatcaac tgctggacaa aatttggcaa tcaaaggtgc aggattagca gggaatgtgt
    28261 atcgagcaac gaatccactc gcttctgcag cgatacatgg atttcaaaag tttcaacaaa
    28321 aacgtacacc acaagaatcg gtaaaaggga ggcaagtggt taatcccttt gaactaggtg
    28381 ggcgttcaaa tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat ggggtagagc
    28441 ctgaaatgtc ttctaccaaa tcgaaaacag agtggaattc tggtctttca aatggtttga
    28501 ttccaaattc gtccatgaat tcagtaagac caaaacaaca ggtacaatca ttggcacaat
    28561 tgagtaagca acaagggaat aatcaaagaa aaccattgtt aaataaaggt atcgcttttg
    28621 ttggtcaatc gattccgttg ccttctggaa aagtacccgt gccacaatta acagctgctg
    28681 gttctctttc acaacagatt cgccaagtga aaaatcaggc gaaaaatggg agttatccag
    28741 caatgcagca gttattgaac caatactctg ttcctaattc gtcggtagcc actctatcag
    28801 gaaatcgttc tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg tcgatttccg
    28861 caccaaaatc gttacaacct gcattttctt ctttagataa gccaacagag caatcggaaa
    28921 aaatagaaaa ggatcctaaa acactgccga ttttggacgc aaaaccagga actgatttta
    28981 ctttaccaaa aggacgaaca gtccaaaaac cattatcgaa gaaaggatga ggaagaaatg
    29041 aagaaagggt gtctaggttg tcttcttttc tttattcttc ccttttttct attgccattc
    29101 tttttttcag gagaactttc tgagcatgga ggagaagcta caggtctagc tgtcgctttt
    29161 aatggggaat ataccgaagg attaccgatt ttttcagaga ttaaaggacg tggtccaatc
    29221 acagatgaac atgcgcaagc agcagttggt gcagctgtaa agtatcagtt attaccgagt
    29281 gtgattcttt ctcaattagg ctatgaatca gcttatggac agtcacaatc tggaagaaac
    29341 gatacgaact tttttgggat tacctggttt cctggttgtc cctacccaca aggttctgca
    29401 cgtggaattg gtggttctga aggtggtttc tatatgaagt ttccgaactt aaaagcttgt
    29461 tatgactatt atggctttat ggtggcgagc caaagcaact ttaatcaatg tgtgggcaat
    29521 aaagatcctg gaagttgttt attgattcta ggtcgtggag gctatgcggc agcaggtatt
    29581 accgaaggga gtccgtatta tcgaggagca atgagtatca taacgtccta taacttaacg
    29641 gaatatgata cttttgcgat caagcattgg aatagcctac caacaaaaaa aacttcgctt
    29701 attgcaggaa aaagtaaagg caatgcgaag gttttggatc ggatcattgg acgatccgta
    29761 aataatgggg aatgttatgg tggtacggca tggtatgtat ctcaattagg tggcccacag
    29821 ttaatgggtt ctggacacat gtttgcgatg ttaatagggg aagattataa ctggacaacc
    29881 tatggttggc aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg agatgtggtg
    29941 aattggcaag caggtggtgc attgtctcct ggaatctatg gtcacaccgg aattataaca
    30001 gatgtttcat atggcggaca acatttctct agtgtggaac agaatgccga gcaaggacgg
    30061 attatggctc ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct ggttcgtaag
    30121 gtgaaataag aaaggcagtg actagaaacg tgaaaaaagg gataatttta gtatttagtt
    30181 ggttatgttt gataggtctt actggttgtc attggttttc ttctgaaaaa caaaaaacac
    30241 aattgagaac gacagaagaa acaatcgaag cagtaaaaga aacaagtgga actcttcaaa
    30301 gtactgaaag taacagtcaa attggttcat tagaagacga gacagcgcaa caaatgcaaa
    30361 tagatgaaat gacaaaaaat ttcttaaaag attttgctag aaaatggagt aatttcacag
    30421 atgtttataa acgcaatcaa gctgtgaggg actatatgac agatcgttgt attaaagaca
    30481 atagtattga tgtagacccg catgtacaaa tgaaaacaac tgggaaactt taccaggtga
    30541 cacaagatat agagaatccg gatcaatatt tgttatttgg agaagaaaca atgaataagg
    30601 ttactcaatt tgtattactt caattgaaag taggtcaaga agctcaaaag attgatgcga
    30661 tcaagattta ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc atatttatta
    30721 tacaccgaag aaatagaaaa aaacagactg tttttttaag aaaaactatg tcattcttta
    30781 cttgaaaaaa gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta
    30841 tgaataacta taagaactat tctatttttt aggagatgga tacgagatga aaaaactaat
    30901 tggctatagt gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat cgactgaatc
    30961 tgcttctgcg gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa
    31021 aggctatccg tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg
    31081 cgttgtagat agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca
    31141 ctagaaggag gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt
    31201 taaaagattc tctttctaag attgaatatg ttgataggca agaaattcaa aaactaattg
    31261 atgatactat tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt
    31321 tatctaaagt aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata
    31381 gacaaactag tctgatatgg gatcatttaa aagacttgta tgacaagtct aaaagaagtg
    31441 aacggactgc tgtatcaatc ttaaaaggcc tttggggaat gaattcataa acaagatatt
    31501 taaaaagtaa cttatttttt atggaggaga aattatgaaa agaataatta aaatatttgg
    31561 tgttttaggt gttttcttat taggtttaag tacttttgtt agtactggaa caagtgtgta
    31621 tgccacagaa aaagtgaata cagttgattt agaaataaag aataaattag tagatgaatt
    31681 aactttttat tttgatacag ttggtttttt agacgaacat ggtgtatacc atataaagga
    31741 ttttggtgct attcgtgaac gaatcagtca agataacgaa caaggaagag cagcaagagt
    31801 cttttatgaa catattattg ttcctagaat ggagaaagtt gattggaaac gatatgctgg
    31861 atgtgtagcc attaattctc ttcctggagg aggagtggct tgggctatag gtacgcaggg
    31921 agattttatt gaagcattaa aagcttttaa ttttgaaaaa gctgcggatt tattattaaa
    31981 aattgcagaa aaacaattag gaaaagctgc agctaaagag ttagctaagg taaatattgt
    32041 agcaggaatt gcagcagctg ctgctagctg tgcattagag taaaagttta tgaaaagttt
    32101 agaacaagag catatggctt ttaaacaagc aatgtttaaa gaaaatatct atttaaacca
    32161 taactatatt cgtgtttcta aggcatgttc accagtattg aatatgcttg gaggtggaaa
    32221 tggattatat catttactgt ttgtcgatgt ttgttggtta atttttttac cagatgaact
    32281 agttatagtc aatgaaaaaa taacaagtaa aaatgaagtg tttaattatt ctttaacaag
    32341 aattaattac aaagagatta ccaagttttc actagaaaaa gttccttttt ggggagaatt
    32401 ttgtttaaaa attaaatgta actggaaaag a
//
LOCUS       DADVXN010000018        32293 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.018, whole genome shotgun sequence.
ACCESSION   DADVXN010000018 DADVXN010000000
VERSION     DADVXN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.018"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(231..611)
                     /gene="yajC"
                     /locus_tag="KB942_000767"
     CDS             complement(231..611)
                     /gene="yajC"
                     /locus_tag="KB942_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBB6851215.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(708..1853)
                     /gene="tgt"
                     /locus_tag="KB942_000768"
     CDS             complement(708..1853)
                     /gene="tgt"
                     /locus_tag="KB942_000768"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBB6851216.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2071..2769
                     /locus_tag="KB942_000769"
     CDS             2071..2769
                     /locus_tag="KB942_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6851217.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2937..5576)
                     /locus_tag="KB942_000770"
     CDS             complement(2937..5576)
                     /locus_tag="KB942_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6851218.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6195..7226)
                     /gene="queA"
                     /locus_tag="KB942_000771"
     CDS             complement(6195..7226)
                     /gene="queA"
                     /locus_tag="KB942_000771"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBB6851219.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7419..8594
                     /locus_tag="KB942_000772"
     CDS             7419..8594
                     /locus_tag="KB942_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6851220.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8598..9239
                     /locus_tag="KB942_000773"
     CDS             8598..9239
                     /locus_tag="KB942_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851221.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9236..10153
                     /locus_tag="KB942_000774"
     CDS             9236..10153
                     /locus_tag="KB942_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6851222.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10150..10821
                     /locus_tag="KB942_000775"
     CDS             10150..10821
                     /locus_tag="KB942_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851223.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11362..11850)
                     /locus_tag="KB942_000776"
     CDS             complement(11362..11850)
                     /locus_tag="KB942_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBB6851224.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(12051..12317)
                     /locus_tag="KB942_000777"
     CDS             complement(12051..12317)
                     /locus_tag="KB942_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6851225.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12317..12580)
                     /locus_tag="KB942_000778"
     CDS             complement(12317..12580)
                     /locus_tag="KB942_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6851226.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13056..13241
                     /locus_tag="KB942_000779"
     CDS             13056..13241
                     /locus_tag="KB942_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6851227.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13383..15206)
                     /locus_tag="KB942_000780"
     CDS             complement(13383..15206)
                     /locus_tag="KB942_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBB6851228.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15804..18509)
                     /locus_tag="KB942_000781"
     CDS             complement(15804..18509)
                     /locus_tag="KB942_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBB6851229.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18815..19762)
                     /locus_tag="KB942_000782"
     CDS             complement(18815..19762)
                     /locus_tag="KB942_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBB6851230.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19952..20221
                     /gene="rpsN"
                     /locus_tag="KB942_000783"
     CDS             19952..20221
                     /gene="rpsN"
                     /locus_tag="KB942_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6851231.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20397..20759)
                     /locus_tag="KB942_000784"
     CDS             complement(20397..20759)
                     /locus_tag="KB942_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6851232.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20770..21891)
                     /locus_tag="KB942_000785"
     CDS             complement(20770..21891)
                     /locus_tag="KB942_000785"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBB6851233.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21907..22257)
                     /locus_tag="KB942_000786"
     CDS             complement(21907..22257)
                     /locus_tag="KB942_000786"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBB6851234.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22387..23898)
                     /locus_tag="KB942_000787"
     CDS             complement(22387..23898)
                     /locus_tag="KB942_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6851235.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24220..25572)
                     /locus_tag="KB942_000788"
     CDS             complement(24220..25572)
                     /locus_tag="KB942_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBB6851236.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25725..26801)
                     /locus_tag="KB942_000789"
     CDS             complement(25725..26801)
                     /locus_tag="KB942_000789"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBB6851237.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26997..28742
                     /locus_tag="KB942_000790"
     CDS             26997..28742
                     /locus_tag="KB942_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBB6851238.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(29022..29459)
                     /locus_tag="KB942_000791"
     CDS             complement(29022..29459)
                     /locus_tag="KB942_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBB6851239.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29443..30816)
                     /locus_tag="KB942_000792"
     CDS             complement(29443..30816)
                     /locus_tag="KB942_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851240.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31138..31626)
                     /locus_tag="KB942_000793"
     CDS             complement(31138..31626)
                     /locus_tag="KB942_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851241.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31648..32250)
                     /locus_tag="KB942_000794"
     CDS             complement(31648..32250)
                     /locus_tag="KB942_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6851242.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9237 a         6890 c         5953 g        10213 t
ORIGIN      
        1 gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta
       61 caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga
      121 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
      181 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      241 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      301 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      361 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      421 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      481 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      541 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      601 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      661 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      721 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      781 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      841 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      901 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      961 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
     1021 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
     1081 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1141 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1201 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1261 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1321 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1381 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1441 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1501 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1561 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1621 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1681 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1741 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1801 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1861 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1921 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1981 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     2041 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     2101 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2161 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2221 atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga
     2281 ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg
     2341 acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt
     2401 cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc
     2461 tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat
     2521 tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta
     2581 ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg
     2641 ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat
     2701 ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa
     2761 gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg
     2821 agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt
     2881 cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat
     2941 tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt
     3001 acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat
     3061 agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc
     3121 atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt
     3181 acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg
     3241 acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa
     3301 ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct
     3361 cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct
     3421 ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt
     3481 tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc
     3541 tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa
     3601 cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct
     3661 gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct
     3721 ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt
     3781 tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga
     3841 gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt
     3901 gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca
     3961 tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct
     4021 ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt
     4081 tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca
     4141 atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg
     4201 cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt
     4261 tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt
     4321 actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt
     4381 acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc
     4441 gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa
     4501 tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt
     4561 tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact
     4621 gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt
     4681 gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa
     4741 gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc
     4801 gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa
     4861 ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc
     4921 atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat
     4981 accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt
     5041 tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg
     5101 atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact
     5161 accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt
     5221 ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc
     5281 aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt
     5341 tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta
     5401 aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat
     5461 ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct
     5521 ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta
     5581 ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt
     5641 tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg
     5701 cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc
     5761 atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt
     5821 acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga
     5881 tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg
     5941 taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa
     6001 acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag
     6061 ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag
     6121 agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc
     6181 ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc
     6241 tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact
     6301 aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg
     6361 aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca
     6421 ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt
     6481 ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag
     6541 aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct
     6601 aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt
     6661 aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca
     6721 tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc
     6781 atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc
     6841 cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag
     6901 attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct
     6961 tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat
     7021 aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct
     7081 tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt
     7141 aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca
     7201 aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat
     7261 tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt
     7321 tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata
     7381 gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt
     7441 caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg
     7501 gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga
     7561 tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag
     7621 aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga
     7681 tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc
     7741 aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga
     7801 tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc
     7861 gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc
     7921 caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa
     7981 cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca
     8041 tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg
     8101 caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg
     8161 cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat
     8221 tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata
     8281 attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag
     8341 tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc
     8401 ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg
     8461 aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg
     8521 tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat
     8581 ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa
     8641 aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg
     8701 taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa
     8761 ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg
     8821 gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg ccgattttac
     8881 gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca
     8941 tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga
     9001 tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt
     9061 atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag
     9121 atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc
     9181 taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa
     9241 acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt
     9301 ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc
     9361 agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa
     9421 taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg
     9481 agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct
     9541 tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag
     9601 atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg
     9661 aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga
     9721 tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg
     9781 cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt
     9841 ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg
     9901 gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga
     9961 tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt
    10021 aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga
    10081 taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt
    10141 tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc
    10201 caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat
    10261 ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt
    10321 aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc
    10381 atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta
    10441 tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac
    10501 aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg
    10561 gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt
    10621 gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt
    10681 atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact
    10741 gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca
    10801 gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt
    10861 ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc
    10921 ctccttatgg gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt
    10981 catactgcca acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag
    11041 gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa
    11101 atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt
    11161 catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac
    11221 tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct
    11281 catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt
    11341 cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg
    11401 atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga
    11461 aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt
    11521 catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa
    11581 tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt
    11641 atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg
    11701 attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga catttccaat
    11761 tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga
    11821 tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg
    11881 tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa
    11941 ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct
    12001 ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa
    12061 tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact
    12121 cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca
    12181 aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt
    12241 ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg
    12301 actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac
    12361 tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg
    12421 tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat
    12481 cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat
    12541 ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg
    12601 atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc
    12661 agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt
    12721 ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac
    12781 aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa
    12841 tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac
    12901 gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc
    12961 tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg
    13021 cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa
    13081 aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg
    13141 aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa
    13201 attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat
    13261 ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg agaataagac
    13321 aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg
    13381 gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat
    13441 cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt
    13501 acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg
    13561 atatctacat aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa
    13621 aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca
    13681 tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc
    13741 aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca
    13801 taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga
    13861 tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg
    13921 tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta
    13981 atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc
    14041 cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt
    14101 tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc
    14161 ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct
    14221 tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct
    14281 aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct
    14341 ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg
    14401 ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca
    14461 gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc
    14521 ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc
    14581 ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta
    14641 tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat
    14701 aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt
    14761 gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca
    14821 gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg
    14881 gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca
    14941 tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata
    15001 acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg
    15061 acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc
    15121 aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct
    15181 actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa
    15241 gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa
    15301 gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa
    15361 acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa
    15421 taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt
    15481 ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag
    15541 ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac
    15601 caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca
    15661 gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa
    15721 aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa
    15781 cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg
    15841 aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca
    15901 acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct
    15961 gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg
    16021 aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact
    16081 aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg
    16141 atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg
    16201 acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc
    16261 attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg
    16321 tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc
    16381 cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa
    16441 agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct
    16501 agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg
    16561 tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat caagtccgca
    16621 gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg
    16681 gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat
    16741 ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg
    16801 acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt
    16861 gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc
    16921 gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca
    16981 gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct
    17041 gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc
    17101 aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg
    17161 ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat
    17221 ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca
    17281 ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt
    17341 tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct
    17401 gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt
    17461 ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc
    17521 tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag
    17581 ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg
    17641 tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca
    17701 aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt
    17761 tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt
    17821 tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc
    17881 gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg
    17941 ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt
    18001 ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc
    18061 ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca
    18121 ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa
    18181 tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct
    18241 aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct
    18301 tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca
    18361 tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc
    18421 gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat
    18481 aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa
    18541 ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt
    18601 tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga
    18661 ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta
    18721 tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg
    18781 tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt
    18841 tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag
    18901 cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc
    18961 cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt
    19021 cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg
    19081 gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa
    19141 ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg
    19201 aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga
    19261 ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta
    19321 tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa
    19381 ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta
    19441 cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta
    19501 ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc
    19561 catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac
    19621 gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga acatgtccag
    19681 aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac
    19741 cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg
    19801 cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt
    19861 tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac
    19921 gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc
    19981 aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca
    20041 aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa
    20101 tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat
    20161 aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta
    20221 atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa
    20281 aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt
    20341 ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat
    20401 ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct
    20461 gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt
    20521 ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc
    20581 ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc
    20641 gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct
    20701 tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata
    20761 ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc
    20821 atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt
    20881 tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag
    20941 ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa
    21001 accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc
    21061 tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt
    21121 gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag
    21181 tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat
    21241 tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc
    21301 gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc
    21361 tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc
    21421 tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat
    21481 cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac
    21541 agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac
    21601 agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc
    21661 agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc
    21721 tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac
    21781 tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat
    21841 agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc
    21901 ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact
    21961 gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt
    22021 taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa
    22081 agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga
    22141 ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc
    22201 gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg
    22261 cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc
    22321 acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt
    22381 ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt
    22441 accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg
    22501 ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt
    22561 gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc
    22621 agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc
    22681 ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt
    22741 ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg
    22801 acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc
    22861 atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat
    22921 acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc
    22981 tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct
    23041 cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc
    23101 tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg
    23161 tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa
    23221 tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt
    23281 cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc
    23341 tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat
    23401 tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa
    23461 tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg
    23521 tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc
    23581 tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc
    23641 ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg
    23701 atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt
    23761 ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat
    23821 aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc
    23881 tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt
    23941 ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc
    24001 acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag
    24061 gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca
    24121 acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat
    24181 tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg
    24241 ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc
    24301 ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac
    24361 aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata
    24421 aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt
    24481 taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt
    24541 tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac
    24601 atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat
    24661 agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat
    24721 cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt
    24781 ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac
    24841 atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt
    24901 cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat
    24961 ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc
    25021 gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt
    25081 ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat
    25141 cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt
    25201 ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat
    25261 tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt
    25321 caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct
    25381 ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg
    25441 cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac
    25501 attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat
    25561 tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg
    25621 gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa
    25681 tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca
    25741 aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa
    25801 tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat
    25861 cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg
    25921 caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct
    25981 ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata
    26041 tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc
    26101 cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca
    26161 acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg
    26221 ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga
    26281 taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc
    26341 ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc
    26401 atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc
    26461 aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga
    26521 aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct
    26581 tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt
    26641 tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact
    26701 tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca
    26761 tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt
    26821 ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact
    26881 tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct
    26941 agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg
    27001 cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg
    27061 tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac
    27121 taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt
    27181 ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa
    27241 cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt
    27301 tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga
    27361 acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg
    27421 atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag
    27481 ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct
    27541 taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc
    27601 gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt
    27661 ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg
    27721 gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga
    27781 aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc
    27841 atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa
    27901 aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat
    27961 ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa
    28021 aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg
    28081 tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga
    28141 caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat
    28201 tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc
    28261 ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt
    28321 ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag
    28381 aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga
    28441 tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt
    28501 tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg
    28561 ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac
    28621 ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat
    28681 tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt
    28741 agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat
    28801 tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac
    28861 cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg
    28921 aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata
    28981 aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt
    29041 aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt
    29101 atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc
    29161 gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt
    29221 gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt
    29281 ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat
    29341 tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct
    29401 cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt
    29461 ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac
    29521 attgatccat tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt
    29581 cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag
    29641 cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac
    29701 gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct
    29761 gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat
    29821 caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt
    29881 taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg
    29941 caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct
    30001 aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct
    30061 tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag
    30121 caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct
    30181 caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag
    30241 atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta
    30301 ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg
    30361 caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc
    30421 atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac
    30481 aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa
    30541 tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa
    30601 cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa
    30661 ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa
    30721 aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt
    30781 aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt
    30841 ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata
    30901 tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac
    30961 ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc
    31021 ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt aggagctctg
    31081 taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta
    31141 tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat
    31201 aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc
    31261 ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca
    31321 gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact
    31381 taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct
    31441 ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg
    31501 ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg
    31561 ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt
    31621 ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa
    31681 gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat
    31741 aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc
    31801 aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag
    31861 caattcatca aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc
    31921 atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata
    31981 taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg
    32041 caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt
    32101 gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata
    32161 agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca
    32221 tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt
    32281 aaaactgcct cct
//
LOCUS       DADVXN010000019        31378 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.020, whole genome shotgun sequence.
ACCESSION   DADVXN010000019 DADVXN010000000
VERSION     DADVXN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.020"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            147..1016
                     /locus_tag="KB942_000795"
     CDS             147..1016
                     /locus_tag="KB942_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBB6851243.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="KB942_000796"
     CDS             1225..2250
                     /locus_tag="KB942_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6851244.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="KB942_000797"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="KB942_000797"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBB6851245.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="KB942_000798"
     CDS             3333..5036
                     /locus_tag="KB942_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6851246.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="KB942_000799"
     CDS             5038..7071
                     /locus_tag="KB942_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6851247.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="KB942_000800"
     CDS             7082..7522
                     /locus_tag="KB942_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6851248.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="KB942_000801"
     CDS             complement(7722..8849)
                     /locus_tag="KB942_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851249.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="KB942_000802"
     CDS             complement(8852..9529)
                     /locus_tag="KB942_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851250.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="KB942_000803"
     CDS             9793..11559
                     /locus_tag="KB942_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBB6851251.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="KB942_000804"
     CDS             11574..12011
                     /locus_tag="KB942_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6851252.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="KB942_000805"
     CDS             12024..13175
                     /locus_tag="KB942_000805"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBB6851253.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="KB942_000806"
     CDS             complement(13237..13935)
                     /locus_tag="KB942_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBB6851254.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="KB942_000807"
     CDS             complement(13928..15280)
                     /locus_tag="KB942_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBB6851255.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="KB942_000808"
     CDS             complement(15503..16903)
                     /locus_tag="KB942_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBB6851256.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="KB942_000809"
     CDS             17064..17801
                     /locus_tag="KB942_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBB6851257.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="KB942_000810"
     CDS             17926..18405
                     /locus_tag="KB942_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBB6851258.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="KB942_000811"
     CDS             18402..19169
                     /locus_tag="KB942_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851259.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="KB942_000812"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="KB942_000812"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6851260.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="KB942_000813"
     CDS             complement(20072..20674)
                     /locus_tag="KB942_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6851261.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="KB942_000814"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="KB942_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="KB942_000815"
     CDS             21355..21939
                     /locus_tag="KB942_000815"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6851262.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="KB942_000816"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="KB942_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBB6851263.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="KB942_000817"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="KB942_000817"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBB6851264.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="KB942_000818"
     CDS             23840..24019
                     /locus_tag="KB942_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851265.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="KB942_000819"
     CDS             24041..25066
                     /locus_tag="KB942_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBB6851266.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /locus_tag="KB942_000820"
     CDS             25257..26501
                     /locus_tag="KB942_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBB6851267.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="KB942_000821"
     CDS             26654..27601
                     /locus_tag="KB942_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6851268.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="KB942_000822"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="KB942_000822"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBB6851269.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="KB942_000823"
     CDS             complement(28503..28745)
                     /locus_tag="KB942_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851270.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="KB942_000824"
     tRNA            28866..28938
                     /locus_tag="KB942_000824"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..29695)
                     /locus_tag="KB942_000825"
     CDS             complement(29057..29695)
                     /locus_tag="KB942_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6851271.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29818..30450)
                     /locus_tag="KB942_000826"
     CDS             complement(29818..30450)
                     /locus_tag="KB942_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6851272.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30801..31100
                     /locus_tag="KB942_000827"
     CDS             30801..31100
                     /locus_tag="KB942_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6851273.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10723 a         5459 c         6309 g         8887 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29641 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29701 taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc
    29761 aatgtttttt tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta
    29821 tactactttt atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt
    29881 acaatttttt agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc
    29941 gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat
    30001 tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt
    30061 actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa
    30121 cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt
    30181 cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat
    30241 acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat
    30301 agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag
    30361 gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa
    30421 gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat
    30481 attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc
    30541 gataacgata gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat
    30601 taggcgtaat tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt
    30661 ttttaaaata aaaatcgttg ccataacagt gtattgaaag gttttaacaa tccagtaatg
    30721 atttaaaaaa atctagatgt agcgtatact ttgtttatag aatgtagtgg gaagaaatat
    30781 atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat
    30841 tatatgatct cgttttaaat ccagagatca aagaaaagga acgtatactt ttgatagacg
    30901 caaagacagg cttagaaaaa ggtcagtatt atcctaaagt cataaataat ttggaacgta
    30961 gtttacgtcc gttagcaatc aggggagaat tgtctaaacc agtatcaccg ttttatatgg
    31021 agatatcaac aataggaaaa tttgaaaaag aattaggtcg aggaatggct tcggcaccga
    31081 ttacttttgg gcatttataa aaacgagtta ttcatctcaa tgaattaaaa agtatagtca
    31141 atctcataat gcaggctagg ttcatttatt tgaacagagt ctgtattttt ttattctaaa
    31201 taaagaaaca gatgtttctt tatattgtat tttatccttt aattaacaca aaaacagcta
    31261 gtataaacta ataatgttaa ttaaaagtgt attttatgaa actatcgtta tttattaatg
    31321 ttgcattttt gtaagttaaa aattggaaat atagattgaa aaaatgaaag caataagt
//
LOCUS       DADVXN010000020        31294 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.021, whole genome shotgun sequence.
ACCESSION   DADVXN010000020 DADVXN010000000
VERSION     DADVXN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.021"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            80..355
                     /locus_tag="KB942_000828"
     CDS             80..355
                     /locus_tag="KB942_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBB6851274.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            361..1128
                     /locus_tag="KB942_000829"
     CDS             361..1128
                     /locus_tag="KB942_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBB6851275.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1296..3125
                     /gene="typA"
                     /locus_tag="KB942_000830"
     CDS             1296..3125
                     /gene="typA"
                     /locus_tag="KB942_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBB6851276.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3386..5158
                     /gene="recQ"
                     /locus_tag="KB942_000831"
     CDS             3386..5158
                     /gene="recQ"
                     /locus_tag="KB942_000831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBB6851277.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5272..5574
                     /locus_tag="KB942_000832"
     CDS             5272..5574
                     /locus_tag="KB942_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBB6851278.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5577..6740
                     /locus_tag="KB942_000833"
     CDS             5577..6740
                     /locus_tag="KB942_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6851279.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6766..10194
                     /locus_tag="KB942_000834"
     CDS             6766..10194
                     /locus_tag="KB942_000834"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBB6851280.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10215..11339
                     /locus_tag="KB942_000835"
     CDS             10215..11339
                     /locus_tag="KB942_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851281.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11329..11766
                     /locus_tag="KB942_000836"
     CDS             11329..11766
                     /locus_tag="KB942_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6851282.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11750..12088
                     /locus_tag="KB942_000837"
     CDS             11750..12088
                     /locus_tag="KB942_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBB6851283.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12207..12767
                     /gene="rsmD"
                     /locus_tag="KB942_000838"
     CDS             12207..12767
                     /gene="rsmD"
                     /locus_tag="KB942_000838"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBB6851284.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12764..13255
                     /gene="coaD"
                     /locus_tag="KB942_000839"
     CDS             12764..13255
                     /gene="coaD"
                     /locus_tag="KB942_000839"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBB6851285.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13245..14288
                     /locus_tag="KB942_000840"
     CDS             13245..14288
                     /locus_tag="KB942_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBB6851286.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14447..15175
                     /locus_tag="KB942_000841"
     CDS             14447..15175
                     /locus_tag="KB942_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBB6851287.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15291..15536
                     /locus_tag="KB942_000842"
     CDS             15291..15536
                     /locus_tag="KB942_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6851288.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15533..15751
                     /locus_tag="KB942_000843"
     CDS             15533..15751
                     /locus_tag="KB942_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB6851289.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15887..16426
                     /locus_tag="KB942_000844"
     CDS             15887..16426
                     /locus_tag="KB942_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBB6851290.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16419..18692
                     /locus_tag="KB942_000845"
     CDS             16419..18692
                     /locus_tag="KB942_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBB6851291.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18730..19776
                     /gene="holA"
                     /locus_tag="KB942_000846"
     CDS             18730..19776
                     /gene="holA"
                     /locus_tag="KB942_000846"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBB6851292.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19865..20119)
                     /locus_tag="KB942_000847"
     CDS             complement(19865..20119)
                     /locus_tag="KB942_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBB6851293.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20519..20788)
                     /locus_tag="KB942_000848"
     CDS             complement(20519..20788)
                     /locus_tag="KB942_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851294.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21258..21713)
                     /locus_tag="KB942_000849"
     CDS             complement(21258..21713)
                     /locus_tag="KB942_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBB6851295.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21851..23779)
                     /locus_tag="KB942_000850"
     CDS             complement(21851..23779)
                     /locus_tag="KB942_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6851296.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(23976..24809)
                     /locus_tag="KB942_000851"
     CDS             complement(23976..24809)
                     /locus_tag="KB942_000851"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBB6851297.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25001..25705)
                     /locus_tag="KB942_000852"
     CDS             complement(25001..25705)
                     /locus_tag="KB942_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6851298.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25728..26354)
                     /locus_tag="KB942_000853"
     CDS             complement(25728..26354)
                     /locus_tag="KB942_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6851299.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26680..29055
                     /locus_tag="KB942_000854"
     CDS             26680..29055
                     /locus_tag="KB942_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBB6851300.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29079..29570)
                     /locus_tag="KB942_000855"
     CDS             complement(29079..29570)
                     /locus_tag="KB942_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBB6851301.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29757..30677
                     /locus_tag="KB942_000856"
     CDS             29757..30677
                     /locus_tag="KB942_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6851302.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30831..31175
                     /locus_tag="KB942_000857"
     CDS             30831..31175
                     /locus_tag="KB942_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBB6851303.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        10372 a         5438 c         6413 g         9071 t
ORIGIN      
        1 ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg aagaggaata
       61 tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag attggacaac
      121 agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg
      181 gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg tcaaatccat
      241 tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt ctttatatcg
      301 tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg ggtgagataa
      361 atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa
      421 tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct agtaacaaat
      481 gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga tccagaggcg
      541 aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg aagagtcttt
      601 atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa tttttgtatt
      661 atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga cgtgatgaga
      721 aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga gccattgcct
      781 agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta tcttttcggt
      841 cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg tatgtacgga
      901 tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta tatttcaaac
      961 cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg tatggtaaca
     1021 agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt agcagggact
     1081 tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga
     1141 aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa aaatgaaaac
     1201 attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa aatttgccct
     1261 gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac gatatccgta
     1321 acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat gaactattaa
     1381 aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg gactcaaatg
     1441 cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta gactataaag
     1501 gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc gaagtagaac
     1561 ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc
     1621 ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca atcgttgtcg
     1681 taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac
     1741 tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt tatgcatctg
     1801 cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca accatggcac
     1861 ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat
     1921 tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc ggaatcggac
     1981 gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa ttagacggaa
     2041 cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa cgtctagaga
     2101 ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat atctttgtcg
     2161 gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac attgacgaac
     2221 caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga gaaggtaaat
     2281 tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca gatgtatcct
     2341 tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt ggagaattgc
     2401 atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc
     2461 cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa
     2521 tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg
     2581 aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc ttaacacctg
     2641 cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt tacggaatca
     2701 tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc
     2761 aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc atgagtatcg
     2821 aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga atgattatcg
     2881 gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa caaatgacaa
     2941 acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt
     3001 tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca ccagaaagca
     3061 ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa
     3121 aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag ctggtttctt
     3181 tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata aattcagaaa
     3241 atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag tttttgtttc
     3301 ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa
     3361 aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact ttggctatga
     3421 tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg aaaatgtatt
     3481 aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag ctttactatt
     3541 agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc aggtggacgc
     3601 ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg gttacgaaac
     3661 agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata tcgctcctga
     3721 acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc
     3781 tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa gttatttaca
     3841 gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt tgacagctac
     3901 agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag aaaacaacca
     3961 tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa
     4021 agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta tcatttatgc
     4081 agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg atttttccgt
     4141 gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag aagccttttt
     4201 atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta taaataaaag
     4261 caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat actatcagga
     4321 agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt tctctcctca
     4381 agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag ggcaaaagca
     4441 aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt cctgtcttca
     4501 gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg
     4561 tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc tttcttgctt
     4621 aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag caggatcgaa
     4681 agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac tgatgaaaaa
     4741 ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg gctatcttct
     4801 gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac aagtattgaa
     4861 aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat cggatgaaga
     4921 aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat cagaagcagg
     4981 cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc gaatacgtcc
     5041 atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc ttgataaata
     5101 tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg ataagtagaa
     5161 agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt ttgctcctta
     5221 tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca gatggattct
     5281 ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat caaacgatta
     5341 atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga agaagtggta
     5401 gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc
     5461 ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa tcagttatat
     5521 agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga atagacttgc
     5581 ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc tatttaattt
     5641 tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta ttagaaaacg
     5701 gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt agcttgattg
     5761 ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg
     5821 cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt aaggagatca
     5881 atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc gaatacctaa
     5941 aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac agtgtacaaa
     6001 aagattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt actgcattag
     6061 tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt ttggttctgc
     6121 ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga ggcttttgtc
     6181 taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta cccggacgtt
     6241 tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac gaattaaata
     6301 aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta tttggcaggg
     6361 gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc gattttattt
     6421 atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta gcgttgctat
     6481 tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg ttcaattcgc
     6541 ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat cttggaggaa
     6601 ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa ggaggctcca
     6661 gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac
     6721 gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta
     6781 gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga attaggaatt
     6841 aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt caaagcggat
     6901 gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg
     6961 atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta cggtcttttg
     7021 tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt tgttggaccc
     7081 aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc agctatcgaa
     7141 gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga cgatgccttg
     7201 gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg cggcggcgga
     7261 cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga acgtgcgaaa
     7321 agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata catcgctaat
     7381 cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat ccatcttttt
     7441 gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc
     7501 tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat
     7561 gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga tttttacttt
     7621 atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat tacagatatt
     7681 gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa agaaatcgga
     7741 ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg aatcactaca
     7801 gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta tcgttcacct
     7861 ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt agtgacaccg
     7921 tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga aacagccatt
     7981 cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac aaacattcca
     8041 tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc
     8101 attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg aaacaaaacg
     8161 atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa
     8221 ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga ttacgtaacg
     8281 gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat caaaggacag
     8341 gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct gctagcaact
     8401 cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg tcttcctgaa
     8461 ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg ctttttgaat
     8521 gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa
     8581 atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa tgtattggtg
     8641 gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt tgatagtttg
     8701 aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg taaaatcgca
     8761 gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt
     8821 cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat cattgcaatc
     8881 aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag tgaattgaaa
     8941 gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa tggaatcatc
     9001 acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat gagtgcaatg
     9061 agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt caatggtcca
     9121 cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg ggaagatgtg
     9181 cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg
     9241 catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc tgtcggatta
     9301 ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat gtttggcgat
     9361 atcgttaaag taactccatc atctaaagta gttggagata tggcactatt tatggtccaa
     9421 aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt ccctgaatct
     9481 gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc taaaaaattg
     9541 caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct tttggctgaa
     9601 tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa
     9661 caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta tcaaaaagca
     9721 tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca aggtatacgt
     9781 ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat
     9841 gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt gaatggacaa
     9901 agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac ccgattgaaa
     9961 gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt tttggatgta
    10021 ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg
    10081 aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt atatgtgtct
    10141 tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa ataataagcg
    10201 aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta ttagttattg
    10261 tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg
    10321 atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa gcaactggtt
    10381 tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc caagcctatg
    10441 atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa gctagtcatc
    10501 tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta ctgcaaacag
    10561 ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt acgaaaatca
    10621 ctactatcta tactaatttc acttttgagt accatgatac agaagttgga atcgaactaa
    10681 tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact tttgctattt
    10741 tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat aaagatagtc
    10801 ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat caagcagata
    10861 aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg actttattaa
    10921 atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc tttcaaaacc
    10981 acagtcagct gctactacat gattttttga caaggcctga ggaaatattg tcagaggaac
    11041 gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa ttctcactga
    11101 cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact agtgggctat
    11161 ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc gatccttaca
    11221 accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt tctaaagaaa
    11281 atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt gatcgttaac
    11341 gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt
    11401 aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc
    11461 cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt
    11521 gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt
    11581 gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca aagtctgttg
    11641 gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt
    11701 agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca tgcaattaga
    11761 caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct acagcttaaa
    11821 acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa gaatgaaata
    11881 tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt
    11941 acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga attttgccga
    12001 aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag agctaaatac
    12061 agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt aaataaaaaa
    12121 taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt atactagtac
    12181 cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat ggcgggcgcc
    12241 gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta aaagaatcca
    12301 tattcaatat gattggacca tattttgatg gagagacagt attggatctg tttgcaggaa
    12361 gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta tgcgtagaca
    12421 aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa gaaccagaga
    12481 aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca gcggagaaac
    12541 aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata atcgatcaaa
    12601 tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg
    12661 ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga aaacagattt
    12721 atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac gtgcattatt
    12781 cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac gtgcggctaa
    12841 attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc
    12901 cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta atgtaaaagt
    12961 catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg cagatgcgtt
    13021 gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg ctcagatgaa
    13081 tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag aatatagtca
    13141 cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga gcagctactt
    13201 accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat
    13261 ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt gtagtagttc
    13321 ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa ctaattacgg
    13381 tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt ggtatcaggc
    13441 aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg
    13501 aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa cagtattata
    13561 tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac aagccttata
    13621 aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat ttctttggca
    13681 agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa agtacagaag
    13741 catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc agttatttgc
    13801 ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca gataaaaagg
    13861 caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca gatgttcgta
    13921 ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta gaaatctatg
    13981 aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca
    14041 acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca agtgcggata
    14101 aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat gttaagaaag
    14161 aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa aaattgaatt
    14221 cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat ctagagaaaa
    14281 tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga gttctgtcac aacccctctt
    14341 tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga aaaaattgac gtaattagat
    14401 gttgaaggga cgattttgcc agagctcact gtttcttcaa ttttaaatga aaggagtaat
    14461 gaatgatttt tactggtcat tctttgaaaa aagaacaatg atagaaaaaa tcagcaagaa
    14521 aatactagct tcacccaaat tgatcatttg gatgtctatg atagttctta tcttttgtat
    14581 cgcaagtggt tttttattat caaatttgct attttataga gaaacagaag agactgctgc
    14641 aaaagaaata ctttcttcgt ttagttccag tggagaagat ccatcagctg aaggaaacga
    14701 tgtagaagag gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa gtgtgaagga
    14761 gcctggtatc tattcctttt cttcagaaga acgggtctat gatgtactaa agcgtgcagg
    14821 tggattatta gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga tagaagatca
    14881 gcaagtccta tatatccctg ctgtaggtga agagctgcca gaacatcttg atcagttggc
    14941 atcctcggaa ggaaaacagc ctacagcaga ttccgagcct cccaaaataa atatcaatac
    15001 agccactcca tcagaacttc agcagattcc tgggatcgga tcagtcaaag cgcaagaaat
    15061 cattcgattt agagaagaaa acggatcctt ccagaaagtg gaagacctgc aggagatttc
    15121 tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc gttacaatta aatagaatga
    15181 ttcaaaatcg actggcacga gcaataaatg gacgctaatg gatactaaga ataatccaat
    15241 tctaagagct tggcaggacg aggaggtagt atttttggat aggaaatagc ttgattgttc
    15301 gcaaagaaac attaaaaaaa ccaatgctga atgtctatct ccaaaataaa atatctggga
    15361 ttcatattat gaatacggct gtcagcggca ataacagtca agctctacga gaacgctttg
    15421 ccaaagatgt acttagctat acagcagata aagtatttat tctcattggg acgaatgatc
    15481 tagcagagca taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt ggatgatagt
    15541 tcagctgaaa aaagactata gttctgatca tgctttcttt atcacgccac cagcagtcga
    15601 tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa gaatatatct ctgttctcac
    15661 agaagtagca aaacgccatg attgtgaagt atcgattttt atacggaatt tcttgaacaa
    15721 atggattcga agaattttta taatgggcta atggatgacg gacttcattt cggcagtgaa
    15781 gggtaggatc ttttgtcaga caggatgatt cgttatattt aaagaatatt ttgtttaagc
    15841 aatgagtact ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt tagaaaaatc
    15901 ttcaaaagca tgcgatacga ataaagaaaa agttactgat aagtcacata atgaacgcat
    15961 tccttgggac cagtacttta tggctcaggc agtactgtta tctttacgta gtacgtgtac
    16021 acgtttagaa gtaggagcga cactcgttcg agaaaaaagg atcattgctg gcggctataa
    16081 tggagcagtc agtggagatg tccactgcat agatgaaggg tgttacgtcg tggatggaca
    16141 ctgcttgcga acgatccatg cagagatgaa tgcactccta cagtgtgcaa aacttggtat
    16201 cccgacggaa ggttcagaaa tctatgtcac acattttcca tgtttggcct gtacaaaggc
    16261 tttgttgcaa gctggtgtga aagggatcaa ttatctccat gactatcgaa atgatcctta
    16321 tgcccaagcg ttgatcgaac aattaggtgt atctgtacac caagttgctt tagactcaca
    16381 gcatttttca aaacttcaaa atgaattaac aggaaaacat gtctagcgtt ttagaaaggc
    16441 atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat gattatctgt ttgttgtatt
    16501 acactgacaa ttggggttat cgtttgcttc ttgccggaat ttttttatgg ttttcgctaa
    16561 aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag cataacggta tccatttctt
    16621 ataggctaca agagtattca tttttagagg aaaaaacaac atctgcgcaa attactgtat
    16681 ttacagatac gattcgtgca aatggtaatt ttttgacaat ggaaggaagg ttatcagaga
    16741 ataaacaaaa gctccagctt tcttatcaag taaacacaga agcagaattg aaaacatggc
    16801 ttaacttccg aggaaaggga atagaattga ctgctgaagg cgagttcatc tctccagcaa
    16861 aaaagcggaa tctttataat tttgaccaag aagcttactt caggaaccat agaatttctg
    16921 gacggttcca aattcaaaag attgaacaga tcaccatgca aaatcactgg cgaaactggc
    16981 ttcagagaaa aagaggatct tttatctcct ttgtccaatc tgaatttccg caaaaaacaa
    17041 gtgtatatat taattcttta ttatggggct ataaagatgc aggattcaga gaaccagaag
    17101 aaatttttag ggagagtggt ttgctgcatc tgttcagtct atcgggtcta cacatccaat
    17161 tttatttagg gtggctgtat tatttgtttc gacgtatcgg atggccacta cacgtcagta
    17221 ttattccgtt gagtctacta actatctgtt atgtttctat tgcaggaagt tctatttctg
    17281 ttttgcgtgc tagtttgctg tttttattgc gattgctagt caaattgctg aataagcatt
    17341 attccaccat ggacttattt gcagttgtct tttggcttct tttactattt gatcctcgat
    17401 ttctttttca tgcgggtgga cagcttagtt ttttcatgag ttttttgttt atcctgattt
    17461 cttttgatgg agattggttg aaaaaaacgg cctctcacgt cctgtttact atgatcacga
    17521 tgccgttgat tgtatggcat ttttatgagt ggcctatttt aggtagtctt tttacattac
    17581 tgattgcacc ggtattcaag tttctttttc ttcctggtgt gttatttctc acactcttca
    17641 atcgctggct gccggaaact ttacttttgc tgatagaaga gggattaatc ttatttgagc
    17701 gaataatcga tttttcttca gggtcgtctc tcattatagg acgtttgccc cttgtgctat
    17761 ctgggatgta tattttcgga ttactgattt gtatggatag gttgaaagaa caacctgtga
    17821 aacacttgtt ttatgttggt cttttttctt ttattttttt gactttgcca tttttacgct
    17881 tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt ttgcaaagtc
    17941 cttttcgaaa agaaacgatt ttgatcgata ctggaggtca gttacacttt gaaagagaaa
    18001 agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca tatctaaaag
    18061 gtcaggggat aagtgtaatc gataaactca tcttgacaca cgacgatacg gatcatgtgg
    18121 gagaattacc cacgttatct cagcatttta ctatcaaaaa aatttatatc ggttggggcg
    18181 ctggacgaaa tgagccatta caaaggcatt tatctgaagc tcaaaaaaat ggtactgaga
    18241 tcattgaaat caaacatggg gacaggatca gcggttatta tgatctttat gtattgactc
    18301 cttttgaaaa gggagaggga agaaatgaag attcgattgg tctatggatg gaatataaca
    18361 ataggcgctt tttattttta ggagatttga atcaagcaat ggaaaaacaa ttgcttttga
    18421 tttatcctaa tctgaaagcc gatgtagtca aattaggtca tcacggtagc cgtacgtcaa
    18481 gtaacacaga ttttctttcc catattgaag caaaacatgg gattatatcc tgcggcgtaa
    18541 ataatcgtta tggtcacccg cacagcgaag tattggagac actagaagaa aatcagataa
    18601 aaacatggcg aacggatcaa caaggaatga tcacctatcg ctggcatcca gtttttcatc
    18661 ctcatgggct agtggaaaca atgatagact agtaattctc atctgctcat ggtaggataa
    18721 tttttgaaag tggtgagtaa tttgagtata caagcagagt tacaaaaaat acgtaaagaa
    18781 aaacttgcat cttgttactt gattttagga acagaaaagt ttctccaaga tcaagttcgg
    18841 acagaaattg tcaaaaaaat ccaattagag ggcgaagatg acttaaattt tttaagtttt
    18901 gacatggaaa atgcaagttt agatgaggta gtagctgaag cagagacgtt gccgtttttc
    18961 ggagatcaac ggttagtatt cgtagagaat ccttattttt taacaggtga aaaagtaaac
    19021 aatggaatcg aacaaaatac tgatttattg acggcttatt tgaaagaacc attagaatcg
    19081 actgtattag tattctttgc gccatatgaa aagttggatg aacggaaaaa agtaacgaaa
    19141 caattgaaaa aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa agaagtacgt
    19201 caatatctga tgaatacctt agagaattcc tcaatcaaaa tggaacgaag tgcaattgat
    19261 ttattcttga ggttgaccga cttagattta tccaaaatga tgagagagtt acagaaattg
    19321 gtcctttatg gacaaaatca acagcaaatc acggtaagag aagtagaaga attagttcct
    19381 aaaacgttag aacataatat ttttgacatg acccaatata ttttaaaagg gaagacggaa
    19441 caagctttgc gtttatacga agatttagtt atgcaagggg aagaaacaat caaaattaat
    19501 gcgattttgc tttctcaatt acgtttattt ttacagacaa aatttcttgt aaagattggt
    19561 tatcagcagg caaatattgc tgaaacattg aaaatccatc catatcgtgt aaaacttgcc
    19621 atgcaggaag taaaaaaatt cgatgaacat ttgctggtac agttgtttga tcaattagtt
    19681 gagatggatt atcaaatcaa aacgggacaa atcgaaaaag aattaagttt tcaactattc
    19741 gttttgcgca cagggcagtt gttaagaaat cgataagaag aaagacgagg gcaaagcatg
    19801 cgggaaggtc gtttgttttt gtcctttttt tgacaaataa aaaaatcggc cgaagccgat
    19861 tcttttattt agcgattttt ttgcttaaac gagatttgtc gcggttagct ttgtttttgt
    19921 ggattagacc ttttgattca gccatatcaa tagctttaac agcttcttta tataacgcat
    19981 ctacgttgtc agcacctgaa gctacagcat cttcaaattt tttgattgca gtacgcatag
    20041 cgcttgtttg agatgaattt ttcacattag cgttttcgct tgtacgaaca cgtttgattg
    20101 cagattcaat gtttggcatt agtttcacct ccgataactt aagttcttac gcgtgttacc
    20161 gcataattca acattcatta ttatacctaa aggcagcgtg ctttgcaata tgaacatgac
    20221 aagttttttt ccttaacagt cttttattag gatttttttt cagtaaaaac ggatagtaca
    20281 agctgaaaaa aatcaaaaag acgctattgt gggaggcaat agcgtcaaac caaactagat
    20341 ttttatttat ctatttaaac aaagtttgaa tgggaatgtt tggtactaag ttattatata
    20401 acaaagagaa ccgtttgcaa agtttttttt gaaaagggat acgattccct gttgttcttt
    20461 gtttttctat taagaaactg cagttatttt ataaccagta tcctttgatc agtaaaggct
    20521 attctggtgt accgattatt ttaatgtatt catcgatatt ttttatcaac atttttttta
    20581 tgaccaactg gttccgacgg ttccatttgc cacttacttc cagcacatat ttatttaggg
    20641 gcaaacgtaa gatatatttt gccaaatagc gatctttaag caggccattg atcacacggt
    20701 cctcaagctg caagttgatc ccagtcagca tcattggctg ttcttgtaaa acgtacgtat
    20761 caattaaaag accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa agtaacctga
    20821 aaacaggcac taattcttcc attttgatat tcgttgaagt ggatgcgatc attctttctt
    20881 ttgtccactg taatctagta taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca
    20941 tcgaattcta taggaagtct cattggcacg gcctaaatta ttacaaaaca ttgattaaaa
    21001 aaagcccctg catcgcagga gcatccccta tatcgtttta ataggtcaat caaaaagcag
    21061 tatcaaacac attttgtatc aaatcaggaa ataatagata agctaagccc agaatcaaaa
    21121 gtaccgctag aagaaaccag ccgattgttc ctatgaaata ctttaatttt tcgaacattg
    21181 tatcacctcg ttgtcagtat atcacggatc agtaaattgg gggtgttaag aatctcctaa
    21241 aatagacaaa atttgttcta ttttcctaaa gcaaatactt gtaagataca gtaagtgata
    21301 ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga agacaacacc taaaatcgtg
    21361 ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac acccttggaa
    21421 gataagacag ccactagccc accgcaaaaa acagctaccc agccaatagg ggatttaaag
    21481 gcttctaata gatcacgtag accgatttct cctgttgcga tagggattag aatagccgct
    21541 gtgatgatcg tcaccccaat attgattcct ttgccttgaa gccaagggaa aaaggaacgg
    21601 gaggctggta tgagcttcat aatcaaaaca aaagctgccg ctatcatcag actttgattt
    21661 ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa gccaactttc catgaaatat
    21721 attcctcact ttcttcgaaa caatcataac atgtttttag aaagttacaa aagtaggaat
    21781 tcatccttca agacctattc ataagctgtt tttttcataa aataaacaaa atcataaaaa
    21841 aacactttgc ttatttgaca gaagacagcc gcgctttgta tagtggacaa gacttacatt
    21901 tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa
    21961 ccaatattgt gcttccttcg tgtcctacaa ctccaagagg tagatttaaa aattgcagga
    22021 agttagaagc aatcaacaag agaataacaa gaatcgaaaa aataatattt tgtttgatga
    22081 tccgccgcat ttttaaagat agccgatgac tgtagacaaa tttatttaag tcgcttttca
    22141 ttaagacgat gtctgcgata tccatagcgg catctgtccc ttgccccata gcgacaccta
    22201 aagaagcggc tgctaaagca ggcgcatcat tgactccatc accaaccatc gcatttactt
    22261 ggtaaagttc attctctttt ttgactagtt gtgttttttc ttccggagta cagcttgcat
    22321 agtaatgttc aatccctact tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg
    22381 ttatcatact tgtatggatg ttttgggatt taaagtagga aattacttgt tgggtatttg
    22441 cttttggaat atctagcaga cctaaaacag caattaactg ctgatcttta gaaagataaa
    22501 tcacggtttt gccttgattt tccaaacgtt cgatcatttc cgcaatctct ttgctgatta
    22561 tcatcgtctc aggatcatat gcgtgcttgc caactttcca ttgcgcacct aaataggtag
    22621 tttgtaatcc aaaaccagta atttcttcta ttggtaagta ttggatttct tcaggtattg
    22681 tgcaatcgaa atattgcaaa atcgcatgag caagtggatg cgtcgttttt tgttccatag
    22741 caacaagaag ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca gttacaactg
    22801 gttttccttt agtgagcgtt cctgttttat caaaggcaat cgcttttaat tcagaaagct
    22861 tttctaaatg gataccgcct ttgattaaaa tcccattttt tgcagaattt gaaagagcag
    22921 ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg agaagccaca actaataata
    22981 ccattcctcg ataaaagctt tcatcccaac tccaacctag gaaaaagtgg gggagaagga
    23041 tcattagagg aacagcaagc aaaatcaatt ttacataatt attttcgatt cgttcgataa
    23101 atgaagctgt ttgagttggc gtattctgcg cttcttctac taattggata atcttagcaa
    23161 agacagtttc ggaactcgtt tttgttactc gaatcgtgat gggatttcct tgattcagcg
    23221 ttcctgcata catctcatcg cctaaatatt tttctacagg cacagattca ccgcttaatg
    23281 cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc
    23341 ctttagcgac aaataccaag ttatccacct ttagttgaga aacatctact tcttgcattt
    23401 gtccattttc ttttaataat tgggcttttt gtggttgaag attcatcaaa gcagtaattt
    23461 ctttttgact tttattagtt gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg
    23521 tcaacattgc tccttcaaag taattaccga tcaaacaggc accaatagca gctaaagcca
    23581 tcaataaatc cacattcagt gtccgatcat tccacaattc tttcaagcct tccgatgttt
    23641 gtttatatcc accgatgata atggcactga tataaaaaag tggggtgatg gtaagagaga
    23701 atcggtcgaa gataaaaccg aagatcataa ataaaaggca gccgattgtt gctgcaaaag
    23761 cttttttctg attagtcatc gttattagac ctccttgtca aaacaaggat agcataataa
    23821 aaatgtaatt gataataata acagctaaat gagaacgata atggttatca tttggtgatt
    23881 aagcttgatg ttgtttttat tatttataat gattattatt aaagaaaata aaaaaccaga
    23941 acatttctca aataagagaa gtgttctggt tttttctaca ttgcaataaa ccaataacca
    24001 atcaaaatga taccaaggat aatacgatac cagccaaaag cggtaaagtc gtttttcttc
    24061 aaatagctta gtaagaattt gatagctaag acggaaacca caaaagatac gaatgttcct
    24121 acaagaagaa caacagtttc ttccattttg aatgaattcc cttcagctaa atatttgacg
    24181 atcttcaagc cgcttgcgcc aaacatgaca ggaatcccta agaaaaatga aaactctgta
    24241 gcgacaaaac gcgaagcacc aatcaagata gatcctagga tcgttgcgcc agaacgggat
    24301 gttccgggaa caagtgccaa gacttggaat aacccaacaa gaagagctgc ttggtacgta
    24361 aagtcattca gagaagtcca acgaggtgtt ttgtttttgt tgcgtttttc gacaataata
    24421 aaggcaatac cgtaaacgat caaaacaatg gcaactggta agaacttgtg gaaatttgct
    24481 tctaaccaat catccaaagg caaaccaatg atgatggctg gtatgcaagc aacgatcact
    24541 ttaaaccaaa gtgtccacgt atcttttttt tctaccgttg atttacttgg agaaaatggg
    24601 tttaacttgt gaaaataaag aacgacgaca gccaagatcg cccctaattg gataacgaca
    24661 ttgaacatcg acataaattg tgaactggca tccagtttga tgaattcatc tgctaggatc
    24721 aaatgaccag tactactgat gggcagccat tccgttattc cttcaataat accgagaatc
    24781 actgctttga taatatttgc aaaaaacata aattccttcc ttcccaatta agatacatct
    24841 tatagtatac cgttattttt tgagaaaccc aatctgtttt ttaatattaa gaaaaacttg
    24901 agaaataaag cagataagta cacgaagaga aaaaagaaga caaagccctc gttcagactt
    24961 tgccttttat gattctctgg taaaaattaa aatatcccca ttatagaaac gagtcgcgac
    25021 tatgtgtttt cccgttatcg atataagacg tatcattgat gccgtggata gaaaatcgac
    25081 cgtttgcata agtgaccttt gttacgctgc catttaaaag cccagcacga acaggctgtt
    25141 taggatcgat cagattaagc aaaaaggcga tcgtcaaacc atgagatacg actaaaacat
    25201 ttccaccacc atttttttcg cagtgatggg cgatatcttc aaatcctgtc catacacggt
    25261 ctcgcaatgt ttcataagtt tccgcccacc cagcggtatc taagttgtat acagcattag
    25321 caatttgttc aaaagaaact tctgtggtaa acatttcatc atatgtttta aaattcaata
    25381 cacgagggat cacaccccac atttccatgt catatccacc ttcaagagac ccgaaacacc
    25441 attctctaat ccttttttca ataaaataag gaatttcttt tcccttagaa tgttctccta
    25501 gaataatacg ggcagtttcg attgcacgac cactgtcact agaataagca aatgaaaagt
    25561 cgacattttt taacccgcga cctaaataat gaatgccttc ttgtccttgc tttgtgaggg
    25621 gtgtatcaca ccagccttgt actcgttcaa ttgtattgaa catcgttttt ccgtgtcgta
    25681 cgatataaag tgtagtttct gtcattttat tctcctccta agtcgtttta acgattgaaa
    25741 aaaggatttg tccttttttc ctgttcaatc gtcgttgcat cgccatgtcc tggatagact
    25801 gcaaattcat caggtaatac aaacagctgg gtccgaatac tatgaagtaa ttgttctaaa
    25861 ttccctgtat acagatcggt tctaccgata cttcctctaa acagggcgtc gccagaaacg
    25921 acaaaatcat caaaaataaa gctgacgctt cctatagaat gccctggggt agggacgacg
    25981 cgaaaagaca tgtttcctaa agtataagtt ttcatttcaa attcatattc tgcaggcgag
    26041 acaataacat cagcaatatc atcatgtcta ccgagtccag aaagattcaa aatcggatca
    26101 cctagccatt tttgctctag tgggctgaca taaactggga tttgataata cgcgcgtaaa
    26161 ggttccactg caccgatatg atcgtagtga gtatgggtaa gcaaaatcgc taccggctgt
    26221 tggtttgttt ttttgatttc ttgtttgatt cgttccgcat cttctccagg gtcaatgatc
    26281 aaaagtgctt cttcgttgta gaccaaataa cagttttctt caatcattcc tgtttttaat
    26341 ctttcgatat gcatgtgcat tcctccttga tagccaaaac ttctttcatc cattagtata
    26401 acgcattcat tgctaggaaa caaaaacagg tacttatttc tctttactta tctgaattat
    26461 cagactatat gaaaagtatt tggatattct aaagagaagg aaagtaagtt ttcatagcat
    26521 tttaatatgt ccttgttaca ttagattcat ccaaaaatcc aaattctata tcctatatcc
    26581 ctgcctaatc agcagggtgt ttttttatgt ttggaaagag agctcttttt gctaaactag
    26641 gttttttcag gtaatcttag agagaaagag gtggtggaaa tgatcaaaca gtcaaaaata
    26701 tctgtgcggc acttagtaga atttattttg cgacgtggaa gtatcgacaa tcgtaaaaaa
    26761 agtaaccata ctgcattaga aggagcgaga atccaccgca agctccaaaa agatgcggga
    26821 gaaaactatg aaaaagaagt ctttttaaaa acaacagttc aattagatga atattacctg
    26881 atcgttgaag gccgtgcaga cggtattttc aaaagagacg gtatttatta tatagatgaa
    26941 atcaaaacct ccgaaccacg atttgaagat ttagaaccag aacaagtgga acttttcttt
    27001 catcaagcaa gggtgtatgc ctatatctat agtcaagaac atgatttaca agaaataaat
    27061 ctgcaattga cttacttcca gacggcagaa gaagtgatca cgagaaaggt tgaacatcag
    27121 acaagagagc agttagaaac tttttttaaa aagctgactg aagattataa agaatggctt
    27181 gtttttcaag aaaactggcg cactgtacgc aatgcctctt tgatggcttt gaaattcccc
    27241 catgatgagt ataggaaagg tcagcgtgaa ctagcggtag ctgcttataa aacgatccgt
    27301 acgaaacaga aattgtttgt agaagcacct accggaacag gaaaaacaat ctctacgtta
    27361 ttccccgctt tgaaagctgt aggggaagaa gagggagaaa agatctttta tctaacggct
    27421 aaaacaataa ctagacaagt agctgaagat gctatgacag cattaaaaga tacaggagct
    27481 gaagtaaaaa gtgtcacttt gacggcgaaa gacaagatct gctttttaga tgagacgacc
    27541 tgtaatccag atcagtgccc atatgccaat ggttattaca atcggattaa tgaaggttta
    27601 tgggatcttt tgaatcatga gaatcagatc actcgtgaag tcatcgaaac ttatgcaaga
    27661 aagcatacac tttgtccttt cgaactatca ttagatgtca gtatctggtg tgacgtaatc
    27721 attggggatt acaattattt atttgatcca actgtttatt taagacgttt ttttgaagat
    27781 gaaaaaaacg aggactatct gtttttgatt gacgaagcac ataatttggt gaatcgttcc
    27841 cgagagatgt attcggcaga actatcttat gaaaaaacga aacgaagtaa ggaagctata
    27901 ccaaaggaat tcaaaaaact tcatcgccgt ttcaacaaac tattaaaaga atttgattcg
    27961 atccgagaaa tagcaaaaga agatcattgg gattatcatc atcaaaaagc accggctgaa
    28021 tctttagtaa aggcaggtta tcaattaagc gaaaaaatta aagaatggct ggcagaattt
    28081 cctgagcacc catcacaaga acaattgctt ccttattatt ttgatttgct tcattttttg
    28141 aaggtcagtg aattttatga tgatcactac gaaacaacag tcgaaaaaac gtatcgcgat
    28201 ctgatagtaa aagagttttg tattgatcca agcttgtttc ttgagcagtc gttggataaa
    28261 ggaaaaagca gtattctttt ttctgctagt ttttcaccac tctcttatta tcaggaaaca
    28321 ctggggggac aaaaaagtct ggcttacaaa ctgccgagtc ctttcccaga aggaaaccag
    28381 caagttttca tcgcaaatta cctagagacg acttatcgaa aaagggaaca cagtctggca
    28441 aaattagtag agacgattca tgcttttgct gatggaaagg tcgggaatta tttcgttttc
    28501 tttccttctt atcaatattt agatcttgcg gtggaagcat tcagacagca atatcccgac
    28561 agcaatgtgc taatccaaga aacggacatg aatgaggaag aacgcgaacg atttttaaca
    28621 aaatttcaaa caaatcccgc tgaaacattg attggatttt gcgtacttgg cgggattttt
    28681 tcagaaggaa tcgatttgag aggggaccga ttgatcggca gtatgatcgt aggtgtaggt
    28741 ttgccgcaga tgaatcatga gcaggaactg atcaaatcat attatgatga gaaggaacac
    28801 cttggatttg cttacgctta tcagcttcct ggaatgaaca aagtcttgca agctggtggt
    28861 cgagtgattc gtggcatggc ggatcaagga atcgttgtat tggcagatca acgattttct
    28921 agtttttctt atcgacgttt attcccacaa cattggcaaa cagcaagaga agtacgaagt
    28981 gttggggaat tggaagaagg aatacagcgt ttttggttat caaaagaagc agtaaatcaa
    29041 aaaaacgagg aatgacagac gtcagcctcg ttttttgatt aacgatagtt agtgaattgc
    29101 aactcgattg gtaagtcaag tttacgtaat aaagcaatca cagcttgtag atcatcacgg
    29161 ttcttacctg tcactctaat ctgatcttct tggatttgtg cttttacttt cagtttttca
    29221 tttttgatag ctgtagtgat tttcttcgcg ttttcacgat cgattccgtt gaccaaatcg
    29281 gcagtttgtc gagctttgcc acctagagcg tgttctgatt cggaaaaatg gatatttttg
    29341 attggcacat ttcttttgat cagcttacca aataatacat ctttgatctg ttcgattttg
    29401 aaatcatcat cgctcaatac aactagattg ctgttctcta atttgatttc tactgtagaa
    29461 tccttgaagt caaagcgatt ggacaattct tttgttgttt gctgaatcgc attttttact
    29521 tcttctgtat tgacttctga tacaatgtcg aagctagcat cttttgccat aggaaccctc
    29581 ctgatataca tataaatatt taccatacat ctatcttatc ataggattag ggcagattct
    29641 tacctgtttg ctaaaattct tctcatttga gttcgaagta ttttatttta atagataaac
    29701 tgttattctt acatttggaa taccaaagta ggggagaggt gcttgtgagt aaaattatga
    29761 ctgcgtatca taaaaatgaa agattaaaga aaatagtggt tattttgata accggattga
    29821 ccgctgcggt cgctttaaat tattttttga ttccagccaa tgtcttttct gcagggatga
    29881 atgggatcgc tcaaatcatt tcctcgcttt tatatgaatg gtttcatatc aagatagaca
    29941 caggaacatt tatttttctt ttgaatatcc ctgtgtttgt attagggttt ctcaaagtag
    30001 gaaaacgagc aacgatcctt agttttgtga atgttgtatg tgtatcggtg atgacgatgc
    30061 tgcttccagt cggacagatc actgataata ttttgatgaa tgccctagta ggcggtgttt
    30121 tattaggtct tggcgtagga ttatctttaa aaatgggctt tacaactggt gggatggata
    30181 tcatctctct tgtactttca caaacaactg gaaaaacagt cggaaactac atgctgattc
    30241 taaatggtat cattgtagtc atcgcaggct tcttctttaa ctgggaaagt gccttgtata
    30301 cgattatttc catttatgca atgactcaag taattgatgc catccacacg agtcatcaaa
    30361 aagtaacagc aatgatcgtt accgtcaagc cagaagaggt aacagaagaa ctagcaaaac
    30421 ggatggtacg tggaatgaca ttactgccgt ctgtcggtgg ctattcaaaa aaacaaggga
    30481 acatgatcat gatggtcatc acccgctacg aaatgtatga tcttgatcag atcgttcatg
    30541 aaatcgacga cgatgcattt atcaatattt taccaactca ttcagtattt ggccggtttg
    30601 caaatgaaaa cgaacaaaaa atgtatcgaa caactggcgt attgccagaa ataaaaaatc
    30661 gttcacaaaa aaaatgagga atcaggttta ttttagaata aatttaatct attacatcta
    30721 ataagaacat ttttataaat ttaaacgaga atcttacttc taaattctca tttacaagca
    30781 gctaaaaaaa gagtatgata aataaaaatc gtgttaagga gtgtcgtacg atgagcgaag
    30841 caaaccaaga acggtctgga caagaaatag aactaatcaa agaacgtatt cttgctgcct
    30901 tggaaatggt gattgatcct gaattaggaa tcgacatcgt caatttaggc ttgatttatg
    30961 atatagaatt caatcctgaa aatggagaaa cagtcattaa gatgactcta acaactatgg
    31021 gctgtccatt agcagatatt ttaacagaat ctatccatga cgcattaaaa gaagtgccag
    31081 aagtaacgaa agcagaagtc aaactagtct ggtatccagc atggacgaca gataagatga
    31141 gccgttatgc acggattgcg ttggggatac gatagaaatc ttggctgact agctatgaga
    31201 agcaaatagg aaaaacaaaa aagcctttta tcctttctag aagaaaagag ataaaaggtt
    31261 tctttgttta taaaattttg tctgctatat tata
//
LOCUS       DADVXN010000021        30567 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.022, whole genome shotgun sequence.
ACCESSION   DADVXN010000021 DADVXN010000000
VERSION     DADVXN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.022"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="KB942_000858"
     CDS             complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="KB942_000858"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBB6851304.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="KB942_000859"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="KB942_000859"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6851305.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="KB942_000860"
     CDS             8118..9086
                     /locus_tag="KB942_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6851306.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="KB942_000861"
     CDS             9104..10009
                     /locus_tag="KB942_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851307.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="KB942_000862"
     CDS             10006..10812
                     /locus_tag="KB942_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6851308.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10948..11937
                     /locus_tag="KB942_000863"
     CDS             10948..11937
                     /locus_tag="KB942_000863"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBB6851309.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="KB942_000864"
     CDS             complement(12254..12778)
                     /locus_tag="KB942_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBB6851310.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="KB942_000865"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="KB942_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBB6851311.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="KB942_000866"
     CDS             complement(15031..16017)
                     /locus_tag="KB942_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6851312.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="KB942_000867"
     CDS             complement(16040..16606)
                     /locus_tag="KB942_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBB6851313.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="KB942_000868"
     CDS             complement(16620..17036)
                     /locus_tag="KB942_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6851314.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="KB942_000869"
     CDS             complement(17493..19427)
                     /locus_tag="KB942_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6851315.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19708..20664
                     /locus_tag="KB942_000870"
     CDS             19708..20664
                     /locus_tag="KB942_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBB6851316.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="KB942_000871"
     CDS             20754..21857
                     /locus_tag="KB942_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBB6851317.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="KB942_000872"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="KB942_000872"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBB6851318.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23637..24983)
                     /gene="gor"
                     /locus_tag="KB942_000873"
     CDS             complement(23637..24983)
                     /gene="gor"
                     /locus_tag="KB942_000873"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBB6851319.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25117..26370)
                     /locus_tag="KB942_000874"
     CDS             complement(25117..26370)
                     /locus_tag="KB942_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6851320.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26376..27299)
                     /locus_tag="KB942_000875"
     CDS             complement(26376..27299)
                     /locus_tag="KB942_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6851321.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27520..30012)
                     /locus_tag="KB942_000876"
     CDS             complement(27520..30012)
                     /locus_tag="KB942_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBB6851322.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30019..30486)
                     /locus_tag="KB942_000877"
     CDS             complement(30019..30486)
                     /locus_tag="KB942_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBB6851323.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8830 a         6508 c         5721 g         9508 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcgcttagtt
    22981 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    23041 aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt
    23101 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    23161 aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac
    23221 aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga
    23281 actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat
    23341 ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt
    23401 taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct
    23461 tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt
    23521 atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat
    23581 tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat
    23641 cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct
    23701 tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca
    23761 ataccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt
    23821 tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa
    23881 actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt
    23941 gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct
    24001 ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc
    24061 agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg
    24121 tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct
    24181 aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt
    24241 tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata
    24301 tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc
    24361 attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct
    24421 aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct
    24481 ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca
    24541 ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag
    24601 gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc
    24661 tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt
    24721 tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa
    24781 cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta
    24841 ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact
    24901 tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt
    24961 acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt
    25021 tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc
    25081 gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc
    25141 tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg
    25201 gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa
    25261 tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt
    25321 agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat
    25381 tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt
    25441 agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata
    25501 gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga
    25561 tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat
    25621 caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc
    25681 cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc
    25741 atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg
    25801 gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc
    25861 gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc
    25921 taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc
    25981 tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat
    26041 caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa
    26101 ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt
    26161 cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc
    26221 tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg
    26281 gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa
    26341 ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata
    26401 taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc
    26461 attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg
    26521 aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat
    26581 gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag atcattgctg
    26641 gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta
    26701 ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta
    26761 tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg
    26821 gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca
    26881 cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc
    26941 gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc
    27001 gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa
    27061 gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata
    27121 ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta
    27181 aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct
    27241 gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata
    27301 attttctcct cctacaactt cataatagta gcatagctct aaaaactttc caatacttct
    27361 tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat
    27421 attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag
    27481 tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc
    27541 ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg
    27601 gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt
    27661 ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat
    27721 cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat
    27781 aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa
    27841 gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc
    27901 taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac
    27961 atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt
    28021 tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag
    28081 aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata
    28141 tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg
    28201 aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg
    28261 gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt
    28321 tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa
    28381 tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac
    28441 gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa
    28501 ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac
    28561 acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt
    28621 acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat
    28681 cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt
    28741 taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc
    28801 tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac
    28861 agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag
    28921 aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc
    28981 taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt
    29041 ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc
    29101 tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc
    29161 attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt
    29221 accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg
    29281 cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc
    29341 aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg
    29401 tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt
    29461 caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg
    29521 tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat
    29581 tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg
    29641 agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt
    29701 ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg
    29761 tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac caatcagatg
    29821 ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc
    29881 aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg
    29941 tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa
    30001 aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg
    30061 gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat
    30121 tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg
    30181 aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc
    30241 aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc
    30301 acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt
    30361 gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat
    30421 ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg
    30481 tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata
    30541 gaaagaaaga agaacgcttt ttgttaa
//
LOCUS       DADVXN010000022        30008 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.023, whole genome shotgun sequence.
ACCESSION   DADVXN010000022 DADVXN010000000
VERSION     DADVXN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.023"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..616
                     /locus_tag="KB942_000878"
     CDS             <1..616
                     /locus_tag="KB942_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6851324.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="KB942_000879"
     CDS             613..1386
                     /locus_tag="KB942_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6851325.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="KB942_000880"
     CDS             1383..1781
                     /locus_tag="KB942_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBB6851326.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="KB942_000881"
     CDS             1966..2637
                     /locus_tag="KB942_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851327.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="KB942_000882"
     CDS             2696..3136
                     /locus_tag="KB942_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851328.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="KB942_000883"
     CDS             3148..3816
                     /locus_tag="KB942_000883"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6851329.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="KB942_000884"
     CDS             3880..4503
                     /locus_tag="KB942_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBB6851330.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="KB942_000885"
     CDS             4559..4945
                     /locus_tag="KB942_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBB6851331.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="KB942_000886"
     CDS             5158..6549
                     /locus_tag="KB942_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBB6851332.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="KB942_000887"
     CDS             7160..7528
                     /locus_tag="KB942_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBB6851333.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="KB942_000888"
     CDS             7708..8649
                     /locus_tag="KB942_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBB6851334.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="KB942_000889"
     CDS             8718..9449
                     /locus_tag="KB942_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBB6851335.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="KB942_000890"
     CDS             9593..11773
                     /locus_tag="KB942_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBB6851336.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="KB942_000891"
     CDS             11793..12275
                     /locus_tag="KB942_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBB6851337.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="KB942_000892"
     CDS             12291..13052
                     /locus_tag="KB942_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6851338.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="KB942_000893"
     CDS             13183..14517
                     /locus_tag="KB942_000893"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBB6851339.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="KB942_000894"
     CDS             14824..15309
                     /locus_tag="KB942_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBB6851340.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="KB942_000895"
     CDS             15302..15985
                     /locus_tag="KB942_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBB6851341.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="KB942_000896"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="KB942_000896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBB6851342.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="KB942_000897"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="KB942_000897"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBB6851343.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="KB942_000898"
     CDS             19601..20230
                     /locus_tag="KB942_000898"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBB6851344.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="KB942_000899"
     CDS             20282..20614
                     /locus_tag="KB942_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6851345.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="KB942_000900"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="KB942_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBB6851346.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="KB942_000901"
     CDS             21440..22813
                     /locus_tag="KB942_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6851347.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="KB942_000902"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="KB942_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBB6851348.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="KB942_000903"
     CDS             23868..24308
                     /locus_tag="KB942_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6851349.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="KB942_000904"
     CDS             24452..25153
                     /locus_tag="KB942_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBB6851350.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="KB942_000905"
     CDS             25150..26271
                     /locus_tag="KB942_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBB6851351.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="KB942_000906"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="KB942_000906"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBB6851352.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="KB942_000907"
     CDS             27650..28558
                     /locus_tag="KB942_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBB6851353.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="KB942_000908"
     CDS             28593..28865
                     /locus_tag="KB942_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBB6851354.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="KB942_000909"
     CDS             28876..29922
                     /locus_tag="KB942_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBB6851355.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9981 a         5146 c         6593 g         8288 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa tacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 ttttttta
//
LOCUS       DADVXN010000023        29362 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.024, whole genome shotgun sequence.
ACCESSION   DADVXN010000023 DADVXN010000000
VERSION     DADVXN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.024"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            23..718
                     /locus_tag="KB942_000910"
     CDS             23..718
                     /locus_tag="KB942_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6851356.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1160..1957)
                     /locus_tag="KB942_000911"
     CDS             complement(1160..1957)
                     /locus_tag="KB942_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBB6851357.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1954..3138)
                     /locus_tag="KB942_000912"
     CDS             complement(1954..3138)
                     /locus_tag="KB942_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6851358.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3163..4410)
                     /locus_tag="KB942_000913"
     CDS             complement(3163..4410)
                     /locus_tag="KB942_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6851359.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4625..6370
                     /gene="dnaX"
                     /locus_tag="KB942_000914"
     CDS             4625..6370
                     /gene="dnaX"
                     /locus_tag="KB942_000914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBB6851360.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6390..6704
                     /locus_tag="KB942_000915"
     CDS             6390..6704
                     /locus_tag="KB942_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBB6851361.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6729..7325
                     /gene="recR"
                     /locus_tag="KB942_000916"
     CDS             6729..7325
                     /gene="recR"
                     /locus_tag="KB942_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBB6851362.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7389..8033
                     /locus_tag="KB942_000917"
     CDS             7389..8033
                     /locus_tag="KB942_000917"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBB6851363.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8048..8377
                     /locus_tag="KB942_000918"
     CDS             8048..8377
                     /locus_tag="KB942_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBB6851364.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8391..9329
                     /gene="holB"
                     /locus_tag="KB942_000919"
     CDS             8391..9329
                     /gene="holB"
                     /locus_tag="KB942_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBB6851365.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9365..10189
                     /locus_tag="KB942_000920"
     CDS             9365..10189
                     /locus_tag="KB942_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBB6851366.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10182..10529
                     /locus_tag="KB942_000921"
     CDS             10182..10529
                     /locus_tag="KB942_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBB6851367.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10598..11470
                     /gene="rsmI"
                     /locus_tag="KB942_000922"
     CDS             10598..11470
                     /gene="rsmI"
                     /locus_tag="KB942_000922"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBB6851368.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11579..12700
                     /gene="dinB"
                     /locus_tag="KB942_000923"
     CDS             11579..12700
                     /gene="dinB"
                     /locus_tag="KB942_000923"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBB6851369.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12754..13356)
                     /gene="nrdG"
                     /locus_tag="KB942_000924"
     CDS             complement(12754..13356)
                     /gene="nrdG"
                     /locus_tag="KB942_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBB6851370.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13671..15860)
                     /gene="nrdD"
                     /locus_tag="KB942_000925"
     CDS             complement(13671..15860)
                     /gene="nrdD"
                     /locus_tag="KB942_000925"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBB6851371.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16159..16953
                     /locus_tag="KB942_000926"
     CDS             16159..16953
                     /locus_tag="KB942_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851372.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16943..18985
                     /locus_tag="KB942_000927"
     CDS             16943..18985
                     /locus_tag="KB942_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851373.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19266..19415
                     /locus_tag="KB942_000928"
     CDS             19266..19415
                     /locus_tag="KB942_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBB6851374.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19426..20940
                     /gene="dltA"
                     /locus_tag="KB942_000929"
     CDS             19426..20940
                     /gene="dltA"
                     /locus_tag="KB942_000929"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBB6851375.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20937..22142
                     /gene="dltB"
                     /locus_tag="KB942_000930"
     CDS             20937..22142
                     /gene="dltB"
                     /locus_tag="KB942_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBB6851376.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22187..22420
                     /gene="dltC"
                     /locus_tag="KB942_000931"
     CDS             22187..22420
                     /gene="dltC"
                     /locus_tag="KB942_000931"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBB6851377.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            22423..23691
                     /gene="dltD"
                     /locus_tag="KB942_000932"
     CDS             22423..23691
                     /gene="dltD"
                     /locus_tag="KB942_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBB6851378.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            23832..24914
                     /locus_tag="KB942_000933"
     CDS             23832..24914
                     /locus_tag="KB942_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBB6851379.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24914..25717
                     /locus_tag="KB942_000934"
     CDS             24914..25717
                     /locus_tag="KB942_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6851380.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            25787..26527
                     /locus_tag="KB942_000935"
     CDS             25787..26527
                     /locus_tag="KB942_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6851381.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26538..27542
                     /locus_tag="KB942_000936"
     CDS             26538..27542
                     /locus_tag="KB942_000936"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBB6851382.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(27587..29284)
                     /locus_tag="KB942_000937"
     CDS             complement(27587..29284)
                     /locus_tag="KB942_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851383.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9276 a         5172 c         6049 g         8865 t
ORIGIN      
        1 ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa agtttggatg ggggagcatc
       61 attggatagt agagtcttaa aggaatattt ggataaaaat gattttccga ttatacgcaa
      121 aggatatcat aaatatttga cattcgaagg gatggaagat tcccatacgt atatcttgaa
      181 agagggaacg gtaaaagcaa gtgttatttc aaatgatggg agagaattta accttcgcta
      241 tatcaaagat cttgaaatcg tctctctttt aaaagacgaa tattcacaat tcatcgattc
      301 gccgtataat attcgagttg aatcagaata tgcagagttt tatcgaatca atcgtgtgcg
      361 cttttggcaa aatatcaacc aagacccaaa gctacaacac tatgtaaaag agtattaccg
      421 ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg gaaagctggg
      481 agctgtttgt acgcaacttt acgaattata tgatttattc ggtgtgcgga cagaagaagg
      541 gatgctgatc gatttttcga tcacgaatga agaaatcgcc aaattttgtg gcatctcaac
      601 tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta tcaccgttaa
      661 agaccatcat atcgttattt tgaatatgga cttgttagaa gacaatatta tcttttgatg
      721 aacaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat
      781 agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
      841 ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
      901 cagcttttca tgtttgatga atattgtgac ttgtgaccga ggagttggta ggagaacacc
      961 gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaga gccaagtaaa
     1021 aacagtctct tatgttttta acttggctgg acttatcacc gtagatgtaa gcttttgaac
     1081 gccgtttatt tgcaacaaaa ggggctgtga cacgattttt gtcacagccc tttttttgcg
     1141 aacattcatt tagcaatttt tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc
     1201 tagaatgggt agtacttccg ccaccgccgc ttccaggagg tgggcttttg gggattcttc
     1261 ttgtcgtgac aaaagaattt gtcagacgat cggttttttc agttaatttg atactggatt
     1321 tttctcggaa tggatatttg tagatgcctg atttcaattg ataacgcgaa acagtcacaa
     1381 aataaaagac aagactaagg ataagagcca aaacaacagc aatgatgatt tctactgtcg
     1441 tcaaaacctt atagcgtgta attttgccag tttcttcgtc tacacgataa tgtcctcctg
     1501 gaactccgtc ttccacatat gttgctgatt tagacaggta agcttttgca gcattgtaat
     1561 aggcagcatt tgtcatctga tcataaacat catctaagat cgaatcgatt ctgctatcag
     1621 tcaaataatc gatcatatta ccagaagtcg aaatgtagat ttcacgctgt cccatatcta
     1681 atagcaatac tgagccattt tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa
     1741 agtctcttgg ttcttctgaa ttagatgtag tcgttacgat caagactcgt cctttgattt
     1801 tttcattcag cttttctgcc tgttgttcca aagattggat ttgatctgtt gtgaataacc
     1861 cagcctcatc atttaccgta gctgtatctg catgagtagc caccggccag ccaacactta
     1921 ctagcaaaag aaagaataaa aaaaattttt tcatcatatc aagtaacctc cgatcagaaa
     1981 gaggatcgct agcccagcga aaatcccaag agcggtcagt cccaattttt tctggctgat
     2041 cggcaggaca ccgctgacct ttcctgtttg tccattcatt gcgtagtagt agactttttt
     2101 acttgagtca ttgcttcgat aagtaacaag ccataccggc agtaatacgt aattatttac
     2161 ttcgtttgtg attgaagcag aagttcgcac accggtcaat gttgtatatc catttgcggt
     2221 atcttttaac agcttctctg aatagtcttt taattcagat tgaatctgtg atttgattgc
     2281 ctcatattcg atatctcttt tttctgcttg gaacccggct aaatactggc ttttaaaatc
     2341 aattgcttta tctaacggga aaggttgcac gccttcgacc atcttttgtt gcgtattttt
     2401 tgataaggca ttttttacta gttctttaaa agaaagcttg cctgctcttg aaacagcaaa
     2461 ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat atgcggataa ctgtaccatt
     2521 cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa taagggaaat acacacctgt
     2581 caacttatcg atttgatcct tattgaaaaa agctttggga acaaaccatt ttttcttggt
     2641 ccaagcgaga aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt
     2701 tggcaagaat tctccgctta aacgtcccga tagtacgacg ggattatggc agtagtaaca
     2761 ataagtcgca gcagttgtcg catcagtcac gatctctgca ccacagttgg gacaaagaaa
     2821 aagatctatc gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc
     2881 ctcgtcaacc gctttgcgct cggcttcatt catatcggca agctgttctt gggctgtaaa
     2941 agtaaaatct tcggtaggtg aaggttcttc tttgctttct tcgccagcca tacgcgcttc
     3001 tttttgcttt tgctcatatt ggctgacttc ttcttctgta tagatattca ggcagtattc
     3061 gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat taggacattt
     3121 atgtgtaaga acatccatca gctgcacacc tttctaaata aattaattaa cggggattcc
     3181 tttaaacggt ttaccacagt ttggacagaa tttagggatg ccgttggaaa gatccacgat
     3241 ctcattgcat tcactgcatc gcatttgtag cttaggctga acgttttcta caggtttggc
     3301 agctccgcag ttggaacaga attttccgga attttctgtc ccgcaaactg gacatgtcca
     3361 agtatccgca tttccttgtg cagtttgttg attttgtttt tctgcttgtt gtttgatttg
     3421 ttcttggtta ttttgagctg cttgagaaag ataagaacca tttgcattca ttcccatacc
     3481 tacacccata aagccggtca ttgctcctgc ttcatttttt ccggctgctt ccataccacg
     3541 tgcaatggaa ccttgcacgt agccttcacg tacgctagga tcacctagca tcgccccttc
     3601 gttacgcata ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg ctacagccac
     3661 agaaacaatc tccatcccac gtaattcacg ccaagaatca tccaatgcag tatccatgta
     3721 tttgcttaat tccaaactct tagatggtac ataagaaatc cgttgaccat ctgcagacat
     3781 ctgattgatt gctgattgta gcgctgttag gaattcagct aaatactgct cattgatatc
     3841 attgatttcc acttgtgttt tgtttttagg gattgcgttt gtataaaata aaatcgggtc
     3901 aacgacatga atagaataag tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt
     3961 gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt ccctttattt cttggagatt
     4021 gatataaaaa acttgttgtt tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt
     4081 ttcagcaatt gcttctttca gtgttccatt gaacatggaa ggagctgcgt catttttaat
     4141 ggtgtaataa ccttcctcag cggtataatc gatgattttt ccaccatcga ccaaaagcat
     4201 catcatgttt ggatagacat ggacgactgt tccgtctgtt aaaacatctt ctgttccttt
     4261 acgattggag ccacgtttgt catctttgcg gactttgaca ccttttgtca tgaccgttgt
     4321 atcactcata ttgtctggtt cgatgacttc tagccattga tctgccagac cgccgccaac
     4381 catacttgtt gctgctttga taagacccat aaaaaattcc tcctctgatt cagctagtgc
     4441 caaacaatta ttgtttattt attataccat aggaagatgt gaaacttctc ggactagaga
     4501 cggaattgaa atggaaaatt gcgtattgtt taaatacctg aaaaagccga gaaggatagt
     4561 agttttttta cctaatatca cgtataataa gagatagtga attttttaaa aaagggggat
     4621 ccttatggca tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt
     4681 tggacaaaaa gctgtgacac agacactaaa aaatgctatt gtttcccaca aaacctcaca
     4741 tgcctacttg tttacaggac ccagaggaac cggaaagacg agtgcagcaa agattttcgc
     4801 aaaagcaatc aactgtccga acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg
     4861 tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa
     4921 cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat tatgcgccta caaaggcagc
     4981 gtacaaagtg tacattattg atgaggtaca tatgctttca acaggagctt tcaatgcctt
     5041 gttgaaaaca ttggaagagc caaaagaaag tgtcatattc atcttagcaa cgaccgaacc
     5101 tcacaaaata ccagctacga tcatttcacg gactcagcga tttgatttta aacggatcaa
     5161 tacggcagat attgtcgaac atcttgaatt tatcttgaaa gaatcgacta tcagctatga
     5221 accagctgca ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg atgcattgag
     5281 tattcttgat caggcaatct cgtttagtga aggaacaatt actttagatg atgccatgca
     5341 agtgacaggt agcttaactg acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa
     5401 agaagtacca gaagcattgg aaacattgaa ccaaattctc gcagccggca aagaatcacg
     5461 tcgctttcta gaggatatgt tacaatactg ccgtgacttg ctgatgtatc agcaagcacc
     5521 taacctagtt gaggaacaat cgagtacgat cagaagtaca tttacagaat tggcacaaac
     5581 tgtttcagcg gatcagctgt tttcaatgat aaagattttg agcgatactc aaaatgaaat
     5641 tcgttttacc aatagtgccg ctatttattt ggaagtcgct acagtcaaac ttgcacagct
     5701 accgtctgct tctgttcctt cagcagatgt agcatttgca gatgatcaga tagtaaaaca
     5761 gctacaacaa gagattaagg aactaagaaa cgaaatcaag gaactacgca gtaatacagg
     5821 aattgagaat acaaaagtac agccggtaca gcaaacaaat cataaaaaac agacaagtac
     5881 ctatcgtgta ccgacggaac gagtatataa agttctgcgg gaagcgacaa gacagcatct
     5941 gaaccaagta aaagaagtat gggaagactt gctgatgagt ctatctgtca ctcagcgagc
     6001 aatgcttaaa gcaagcgaac cagttgcagc tggacctaca ggactagtga ttgcgtttga
     6061 ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa
     6121 caacttaagc cgaatgatca aagattatgc gcctgatcct gtttatatta caagagaaag
     6181 ctggccagaa cttcgaagaa attatttgac gcaggcacaa gaagagggaa cagaaaactc
     6241 ttttgcttct gaagactcag aaggagaaat cgagttgctt cctcaagaac aagaagtacc
     6301 agaagaaaca cttgtagtaa aagcagagga actgtttggc tccgaagctg ttacagtggt
     6361 agatgattaa taaaaaggag tagataatta tgatgcgtgg aatgggcaat atgcaaggca
     6421 tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga agcacaagag ctgctaaata
     6481 aaacagagtt tacaggtaaa gccaccaatg aattggtcaa agcggttttt acaggcgatc
     6541 gtcgcatgaa agatatccaa atcaatgaag aagtcgttga tcctgatgat atcgagatgc
     6601 ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat gtggaaactg
     6661 agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt ttagttttaa actgaaggag
     6721 taatgaatat gcaatatcca gaaccaattg cgaaactgat cgacagctac atgaaacttc
     6781 caggaatcgg tcagaaaaca gcgacaagac tcgcctttta tacgatcgat atgaaagatg
     6841 aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa acgcgatttg catttttgta
     6901 gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg
     6961 atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc
     7021 gagaatatca cggactttat catgttctcc atggtgtgtt atcaccgatg gaagggacag
     7081 gtccagaaga catcaatatt tcttctctgt tgcagcggct gcatgatgca gatgtaaaag
     7141 aagtgattat cgcaacaaat gcaacaacag agggagaagc aacagctatg tatctctcac
     7201 gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc acatggtctg tctgtaggct
     7261 ctgatatcga gtatgcagat gaagttactt tattgaaagc tgtcgaagga agacgtgagt
     7321 tataaatttc taattaagtg ttttttcaga tacaatataa aagcagcaca agagatgggg
     7381 gcaacaatgt gaacggacta tttattacaa tcgaggggcc tgatggtgca ggaaaaacca
     7441 gcatcttgaa tgaactttac cctcttttga aagaagtagc aaaaacagaa atcattcaaa
     7501 caagagaacc aggaggcatt ccgattgctg aagagatccg agcagtgatc cttgatccaa
     7561 aaaatgatcg gatggatgaa cgtacggaag cactgcttta tgcagcagca agaagacaac
     7621 atctagtcga aaaggttctg cctgctctgg caaaagggaa aatcgtttta tgcgatagat
     7681 ttgtcgacag ttctcttgct tatcaaggtg caggccgacg catcggcgtt accgagattg
     7741 ctcggttgaa tgagtttgct acagaaggta ctacgccgga ttttaccctt tatcttgacg
     7801 tagattcaga caccggatta agaagaatca agaaaaacag acaaaatcaa atcgaccgtt
     7861 tagattcgga aggtttggaa ttccaccagc gtgtacgtca tgcttactta aagctggcag
     7921 aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc
     7981 aaacaagtta tcatacaatt attgaacagt atccgcaatt ttttgaaaac taggaaggtg
     8041 aacgtttatg aaaattattt tagcgattat ccaagacaaa gacagcaacc gtttatcaaa
     8101 tgaattgatt gatgcaaata tccgtgccac aaaactatca tcgacaggcg gcttccttaa
     8161 ggctggaaac agtacgttta tcgtgggtat tgaagatgac cgtgtagaag aagcattgga
     8221 aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg ttacactgga
     8281 tatttcgatg gatgggggcg tcccttatcc agtagaagta gaagtaggcg gtgccacagt
     8341 cttcgtctta cctgttgaag gattccatca attttaggag gagctgagct atgaatcaag
     8401 aaataaactt ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg agttttgagc
     8461 atgggcgtat cgctcatgct tatctttttg aaggggagaa aggaacagga aaacatgaag
     8521 taggtatatg gcttgcacag cacctgttct gtacccaaat gaaagacaat ctgccttgtg
     8581 gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca tccagatgtt ttggttattg
     8641 aaccggaagg acaaaccatc aaagtcgatc agatcagacg tttgcagacg gaattcagtc
     8701 gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata
     8761 gcagtgcggc aaacagtttg ttgaaattct tggaagagcc ccctggagat tttttagcgg
     8821 tgttagaaac agatgcagta ggaagaattc tccctactat tcaatctcgt tgtcaagttc
     8881 tgcattttca agaactttcg aaagaagcac tgatccatcg attgcaagaa gagcagattc
     8941 cattggaaaa agcaaaactt ttggctttct taacaaacag tttaggaaaa gcagttgaaa
     9001 tctctcaaga tgaatggttt aatgatgcta aggacttgat ttatcaatgg ttcgtttact
     9061 tgcaaaagca agatactcaa gcctttatct atgtccaaaa aaaattggta aaatcattta
     9121 aagaaaaaac acaacagttt acaggattat ctgtcttgat gttttattat caggaagcac
     9181 agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa acagataaac cagataattg
     9241 aacggatatt gatcgcagag cagaaactac gcagcaatgt cagttttcag gctgttgcgg
     9301 aacagttcgt tttgcatacg attttttagc ttgccgcaaa ggatgatgat caaggaggat
     9361 ggaaatggta gaagtagtag gtgtccgata tagagatgca ggtcatatct attattttgc
     9421 accaggagac tctgaatatg tttatagtga caaggtatta gtcgaatctc agcaatcctt
     9481 acatattgct actgttgtcc ttcctaaaaa agagattgat cccaatgacc ttcctgaagc
     9541 tatccgtccg atcttgcata aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa
     9601 aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga
     9661 aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat aaattgattt tttctttcac
     9721 agcggacggg cgaattgact ttagagaatt ggtaaaagat ctggccgctg tatttcgtac
     9781 tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca aagataatag gcggaattgg
     9841 tccatgtggg cgtccattat gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat
     9901 caaaatggca aaagatcaaa gtttatcttt gaatccaaca aaaatttccg gtttatgcgg
     9961 acgcttgatg tgctgtctgc aatatgagag cgaagaatac gagacagcga aaaaagaact
    10021 ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa
    10081 tctgcttagc cgtatcgtta gtattcggct gactggacgg gaaaatcctg tcgaatatga
    10141 atgggatgaa ttgaaagaat ttatccaagt aggtgagatg aatggataag agatcattat
    10201 acgacggatt gaatcaatta gaatcagagt tgagaaatac attaacagaa cttgttgaaa
    10261 tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac tttggagatg gaaaatcaac
    10321 gtctgcggga acacctgcgg gaattgaacc aagttgcaca agctccagct gaaaatgtga
    10381 agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg
    10441 tctgctacga tttatatgga tcaagaaggg acaatgacga gccttgtgcg ttttgtctag
    10501 aagtgatcta tcgcgaaagc ggtcgatgac caataacaag aggagactgg agcggagaaa
    10561 tactgctcca gtctttttat taaggagatt gtgacagatg tataatcaaa aaagtttcaa
    10621 gggaaatccg gtatatgggg atttatatct tgtaccgacg ccaattggaa atttagaaga
    10681 tatgagctac agaagtgttc gtatgctgca agaatccgat cttatcgcaa gtgaggatac
    10741 gcgtaataca caaaagttat tgaatcattt tgaaatccat acaccccaaa aaagtttaca
    10801 tgaacataat tataaagaac gtgtccctca attgattgct ttgttaaaag aaggaaaaac
    10861 gattgctcaa gtcagtgacg caggaatgcc ttccatcagc gatcctggac atgagctagt
    10921 tttagcctgc ataaaagaag ggatttctgt gatctctatc ccaggaccta cagctgggat
    10981 gacggctttg attgcttcag ggctagtgcc tcaaccattt ttattttatg gcttccttcc
    11041 acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta aaagaacaga cagttacgct
    11101 gattttttat gagtcacctt atcggatcac tgctacattg acgaatttct tagaaatttt
    11161 tggaaatcgc caagtcgttt tgtgtcggga attgacaaag gtccatgaag aataccttag
    11221 aggaagtacg gaagagctac tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg
    11281 tctgcttgta gaagggaatt tgttcgaaat gccagtgacg gaagttgatg aagagttggg
    11341 aacgttaaag gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat
    11401 caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc agtaccatga
    11461 attagactga gatagtataa gttgtctata atgaaagact aatgaggaaa tcagctacta
    11521 acttttcaaa tctcttgaac gcgaacgaat gttcttgtat aatgaataag aggtgaggat
    11581 gatggagctg caatttcctt tacataatga tacatcaaga aaaatcattc atattgatat
    11641 ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg gacatccatt
    11701 agtaattgcc agacacccca gtgacactgg tgggcgtgga gtagtgacta cagcaaatta
    11761 tgaagcaaga aaattcggaa ttcattcagc aatgagtgca caaaaagcgt atgagctttg
    11821 tccgcaagcc atctttaagc ctggcgacca tcataaatac agcgatattt ccaaacaagt
    11881 acgggaagta ttctatcgtt atacagatat tgtggaacca gttagtatcg atgaagcgta
    11941 tcttgatgtg accgaaaata aaatcaattg caagtcagcc atcaaaatcg cccgaatgat
    12001 ccaagcagat atttgggaga gtgtccagct aacatgttct gcgggagtaa gttacaataa
    12061 gtttttggcc aaattagcat cagactatca aaaacctaga ggaatcacgg tagttgcccc
    12121 tcaagacgct gtgccatttt taaaagcttt gccaattgaa aaatttcatg gggtagggaa
    12181 gaaaactgta cctagaatgc atgagctagg gatttataca ggaaaagacc tatatgaatg
    12241 cacagaaatg atgttgattc gaaattttgg aaaaatgggc tattctcttt atcgtaaagt
    12301 cagaggaatc catgattctc cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga
    12361 gcatacttat gggcagccgt tgcaaacgga agaagcagtg ctgactcagt tgcgccaact
    12421 agctgaaaaa gtagaagaag cactgagaag agtccaaaaa catgggaaaa cagtagtttt
    12481 gaaggtcagg tacacggact attctactgt gaccaaaaga gtgacattgc cagaatatat
    12541 ctataaaaaa gaagcactct tttatcaagc aagccttata tgggaggaaa tcttgggagt
    12601 agaaaaagga atccgattgt tagggattac tttgacgaat ctggacccga tgacttatga
    12661 aaacatcgtc cttccattat gggaaaatca agaaatataa aaacagcttt gagagttgaa
    12721 accggaaatc cgttttctct cccaaagctg tttttatatc aagtcttgct ttttgatttg
    12781 ttcataggtt tctgttgcat ccgtacattt ttcccaaatc acaacttgat tggattctaa
    12841 agatttttgg acgtcaatga tccgctggtt gctacttccg cgaaattgta gttttaggtc
    12901 acgtttgctt aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa
    12961 tttatcttct gaatcttgta gcaattcatc aaaggtatag cctgtccatg accagatatc
    13021 ttttgtcgta ccgaactctt ttcggatacg tcgtacgact tgcagacaaa catctgtatt
    13081 caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc
    13141 gatgatttgt tcttctaatt caggtgtgaa tggtttgcca tatcgaaaat tttgtgcagc
    13201 tttgttgaag cagccttcgc aggcaaataa gcagccacta acgtaaagac tgttgcggac
    13261 accttcacca tcgacaaaat taaacggttt gtagtcagca atatagtttt ggctgtagtc
    13321 ttttgctaac cattcttttg gttttggatt tctcataaaa aaacttcttt catttaaggt
    13381 tcaaagatat agctattgaa gataaaatct actcgaccac ataaataaga acaaactgac
    13441 ctaaatccat tgttcaagaa agcttgttct tattttgata gagggcgttc aaagatacaa
    13501 agccatcgaa agagggtggt gaaaaaagac ttgtcatagc ctcttattct gaataaacgg
    13561 tgttcaaaag cttgcatctg cagtaataag cccaactaaa cgaaaaatat gaggaactat
    13621 ttttgtttag ctattcttat tacttgatgc aggacagctt tttcacaacc tcatttcatg
    13681 tgtttcacac gtgaggaaat ttctttatgg cggccatgta ccatcggacg agcttgcgga
    13741 tttcctaaat agccacaagt acgtttgact acatcacatg tttttggatc atgatttcca
    13801 cattctggac attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa accacattga
    13861 tagcaatgat cgattggcgt attcgtacca agatacccga ttttgtcata tgcaaaatcc
    13921 catacagctt ctaaagcttt cggattttgt tggagcatag ggtattcaca gtagtggatg
    13981 aatccacctg aacaatattt tggataatcc ttctcaaatt ccaatttttc aaatggcgtt
    14041 gggtttttac gcacatcata gtggaaactg tttgtataat attctttatc tgtgatattt
    14101 tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac ggtcagttag actttcactt
    14161 ggagttgaat agacactgaa atgataacca tattcatccc cccatgcatc tgcattagct
    14221 tttaattctt tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg
    14281 ccaccgaaga aagctgaagc aacttcatac aatccaatgt agcctaacga tacagtagct
    14341 cgacggtttt tgaataattc attgacagaa tcggtacgtg aaagacgttt cccgaaagca
    14401 ccatacatat ataaaattgg cgcatttgct ggaatggctt ctttacaacg ttctacacga
    14461 tagagaagcg catctttcat gatttctaag cggtcagaca gtaactgcca gaatttattc
    14521 tgatctcctt ttgattcgat cgcgattctt ggcaaattca atgtaacaac acctaaattc
    14581 attcgtccga catttacttc ttgaccattt tcgtctttcc acccttgcaa gaatgaacga
    14641 cagcccatag gtactttgaa acttccagtt aattcaacga ttttatcgta attcagaata
    14701 tcaggataca ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc gtaattagga
    14761 tcttcagggt ttaagttcac accttttttc aaagtgaaga tcagtttagg gaagatagct
    14821 gtccgctttt cactccctaa tccattgatt cgaatctgta aaattgcttt ttgaatttct
    14881 ctttcaaacc agttttctcc tagtccaaat cctaatgaag tgaaaggagt ttgtccattt
    14941 gaagtaaata aggtattgat ttcatattcc aaactttgca tagcatcgaa gatatctttt
    15001 ttggtttttg cttttgcata ttctttttgg cgttctggcg aatcgatcca ttcttccgcg
    15061 tcttttagat gttttttata gttcaattct gcaaaaggag caagcaattc atctgttcgg
    15121 tcggcagaac agccaccata ttgacttgac gctacattgg caataatctg tgagatttgt
    15181 gctgtggcgg tctggataga ttttggcgat tcgacttcgg cattgccgat tttgaaccca
    15241 ttgttcagca tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt ataaggatga
    15301 taatccaaat catggtaatg gatatcgcct ttttgatgcg catttgctat atgtggtggc
    15361 aacattttca gtccgattga ttttccaacg atcccggctg tcagatctcg ctgtgtattg
    15421 aaaacattac tgtctttatt tgcgttttcg ttcacgacag tttggtcctt gttgattagc
    15481 ttttcaatcg aaaagttgat atctgttgct ttactgcgag caaaatctcg tttggtacga
    15541 taattgatgt actcttctgc taattcatat tcgttttttt ctagtaatat atgttccacg
    15601 atgttttgaa tctcatagat tttgacattt tcagcaaacc ggcttttgat ttcttggaca
    15661 accaagtctg caatcatttg aattttttca taagtaagtg gtgataatga gccgtgaatt
    15721 ttcttttctg ctttgatcag tgcttcattg atcttttgtt catcgaaatc aacatggcgg
    15781 ccatcgcgtt tgatgacttt catctgggat gtctctggct gctgagtaac gacagttttt
    15841 tcttcataat ttactttcat tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa
    15901 acaaaatcgg ggaaggagca actaaatatc ggctatatat agtattcaat gtgctgtata
    15961 attggatttc tatactatat gtagtgtata ttttattgtg aaaaaaggtg aaaacttgta
    16021 gctggtggat ttgttttttg catatttaat tttgctgaat aaaataattg aataaaattt
    16081 tttattcagt ccaagtaaaa gtgacaaaaa cataagagca aattattcgt agatatgcta
    16141 ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag aagaattgga aaagatgatc
    16201 gaagtgaaac atctaagtaa aagctacggc agcaaacttt ctcccgtcaa agccttagat
    16261 aatatctcat tcacagtaga agaaggcgag tttgttggga ttatgggccc aagtggtgca
    16321 ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat gggcagtgtc
    16381 tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga ctttcgtcga
    16441 aaaaaactag gattcatttt tcaagagttc aatttgatcg ataccctaaa tgcaaaagac
    16501 aatatcctat tgcccctagc agttgaacgg atgactaaag aggaaatgga aaagagagta
    16561 agacatgtag ctcaattgtt gaatatagaa gaactgctca aacggtatcc cgatgaactt
    16621 tctgtcggac agagacaacg aattgcagct gctagagcac ttgttgttca gccacaagtg
    16681 atttttgcag atgaaccaac tggctctttg gactcgaagt cggctacaga attgttgaac
    16741 tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac ccatgatcca
    16801 tatacagcaa gctactgcga tagaatttta tttattaaag atggtgtgat tttttctgaa
    16861 gttgtcagaa gagggacaag aagagagttc tttgagaaag taatagacat gcaggcgacg
    16921 attggcggag gaggaaaaat gaatgctgta taaactagcc gttaaaagtt tttttcggca
    16981 gtcacgaggc tatcttgttt attttttcag tttgacttta tccgtaatga tctattattc
    17041 cttcagcgca atgacctacg atcaatcttt agtcagaaga gcaagtcaag atacttcgat
    17101 agatggtgga ttgagttttg gtggatttat cattactgtt gttttgcttt ttttcgtatt
    17161 cagtgccaac cgatttttct tgaatagaag gcaaaaggaa ataggcatct atcagctttt
    17221 tggtatgaat aaactgcaga tatcaggtat atatgtgtta gaaatcatga taattggatt
    17281 gttcgcctgt atttctggga tattactcgg tattattttt tccaaactct tttcaatgat
    17341 tttggttcga atgatggata tggatcttac tagtccattt tttatctcca ttccatcagt
    17401 agtagatact ttatttgctt ttttcatcat ccttttagca gtatctgtat attcaatatg
    17461 gaaaatatgg cgctatccaa tgattcgaag ttttggagaa aaagaacaag cagatagttc
    17521 aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc atcgggttac tacttttaac
    17581 gagcggatat tttggagcta gtcattttag ggaaatcact tcatggatga tcagttatca
    17641 ttatcttgat acattagttt tcttttttcc ttttttaatt tttttcgttt gtgtggtcgg
    17701 tacttatttg tttttctgtt atacatttcg gttatttttg atcatattga gtaaatggaa
    17761 gatttattac tatggtatca attttttgat aatgggtaac acgcaagttc atttgctcaa
    17821 aagttggcgt acaaattctc tgattacagt cattctcgga atttctttaa tgacaattgg
    17881 cggcacggct agtatctcta ctattttatc ctaccaggag aaagtttcct ccccgatgga
    17941 ctatcagttt gatgttgaga cagagaaaaa aatcaagccg attctagcag aggaagggca
    18001 gaagataact aaagaaatca ccttgcatta taaagtcatt gggagtatct atagtcaaaa
    18061 tctcgaagaa cctatgtttc aaactgctaa tctgatcacg gaaacacaat atcaagcctt
    18121 cagaaaagtt aatccagatt tgccgaacat ttctttgaaa tcacctggtc atactgtttt
    18181 gcttgatcaa atgcaaactc tttttcggaa tatttcatta tatggatcta gtattggact
    18241 gccagataat caagaattga agattcaaca aatgcttcca agtgtattag gtgacgaaac
    18301 aatggtatat ctaagtcctg tactgattgt agatgatacc gtttttgaaa aagcaaaagg
    18361 tgtggattat caagtagtca atgtagatgt atcaggtgga aataatgaaa aaatagatga
    18421 acgactgagc aatgacctgt cgataaagtg gctcaatgct gtttattatt cttatgatat
    18481 cgtaaatgga agtctacaag gagaaattag cacaaagaaa cttccggatg accaaatgaa
    18541 ggaagcacaa tttagccaag aaagctctcg tttgaatttc tcttccagct attcgaaatt
    18601 gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc acactatttg tcgggatgat
    18661 cgtgattatt gctactggta gtattttgac agtccggcaa ttagcagaag cagaggaaga
    18721 aaaagaaaat tatcacctat tgactaagct ggggattcca caaagaagga tcagacggat
    18781 gatcttcgag caaaacgcac ttaccttctt tccacccatg atattaggga tcactcacgc
    18841 agtatttgca atcaatgtct tcactcagta tataaaaact gccgattact ggttggctta
    18901 tcttgtgagt ggcttattga tccttatcta tcttttgctt tatgtcatta cgagtcagtt
    18961 atattgtcgg attatcgagg aatgatatac ggaataagac aagataataa atagaactaa
    19021 attttgaata ccatttattt ggaataagag gttgtgacac gatttttgtc acaacctctt
    19081 tattttttat attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt
    19141 gctattttta taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta
    19201 gacacatgtt tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga
    19261 atccaatgaa caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc
    19321 gaacaatttt ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata
    19381 ttcaaggagg aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg
    19441 attattgaag caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg
    19501 gatgagacgc atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta
    19561 gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg
    19621 attgtctcat ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat
    19681 acacccaagg agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt
    19741 atcggggact ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa
    19801 gaaatcaaga agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat
    19861 ttttatatta tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat
    19921 gataatctag tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg
    19981 catttcttag cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca
    20041 ttagtttctg gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa
    20101 ttattcgcga cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg
    20161 gatatttgct tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt
    20221 ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca
    20281 aatgcacgga tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt
    20341 cagattgacc agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa
    20401 gacacgaaag tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa
    20461 atcattattg caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct
    20521 gctgcgttct tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta
    20581 gatgaatatg ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga
    20641 taccggattg aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa
    20701 gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta
    20761 ttagaaaaca aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta
    20821 ctagaagttg taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct
    20881 cagacagtta atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga
    20941 tgttacctca tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc
    21001 ttgtaccgat cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag
    21061 cagtgacact tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg
    21121 cgctgattgg gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc
    21181 aaaggaaaaa tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt
    21241 ttttaacgaa agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag
    21301 gaatctctta tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga
    21361 tcaaaacata taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt
    21421 ctggaccaat cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg
    21481 ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata
    21541 aattcattat cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct
    21601 tacagcatgg tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt
    21661 tgttttttga ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat
    21721 atcagacacc aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga
    21781 atcgttggca tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt
    21841 tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt
    21901 ttgctttgtt tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg
    21961 gactgtatca tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc
    22021 ataaagagca gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc
    22081 aagcagtatg ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct
    22141 aacgaatagt gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat
    22201 tttatcaatc ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga
    22261 tctatttgat gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga
    22321 cggagagctg gatatccaag tccctgtatc tgaattcgag cgagaggatt ggtgcacacc
    22381 gaataaaatc attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt
    22441 gctattttcg gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc
    22501 tatcaagtag atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg
    22561 aacgtgttga aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct
    22621 ttttttggct caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa
    22681 aaataccagc gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc
    22741 caatatatga tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt
    22801 atttctcctc agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat
    22861 tctgaacttc aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc
    22921 tatttagcta gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg
    22981 attctgcaaa cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc
    23041 agccaatgga acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat
    23101 cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa
    23161 ttatccattt tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg
    23221 aaaaacgatt tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa
    23281 aaaaattggg attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa
    23341 ttagcaaaaa accataacga agtattgttc atcatcccac cggttaacga gaaatggagc
    23401 gattataccg gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa
    23461 ttaaatagcc aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat
    23521 tttatggaag acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc
    23581 agaccgtttt tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt
    23641 tttagcaaat cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag
    23701 gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac
    23761 caagtttgcc tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg
    23821 ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag
    23881 gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg
    23941 ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag
    24001 gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa
    24061 ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg
    24121 ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca
    24181 aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt
    24241 tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag
    24301 atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa
    24361 aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta
    24421 aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc
    24481 gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg
    24541 gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag
    24601 gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg
    24661 ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag
    24721 gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg
    24781 ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata
    24841 cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc
    24901 ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt
    24961 ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac
    25021 gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca
    25081 gcacttattg agttttctag tcgataattt acttttattc gcattcaact atgcattgtt
    25141 ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt
    25201 tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa
    25261 ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta
    25321 tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct
    25381 tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag
    25441 tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc
    25501 ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat
    25561 tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg
    25621 tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg
    25681 gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa
    25741 aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt
    25801 tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca
    25861 atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga
    25921 acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat
    25981 tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac
    26041 attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga
    26101 aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct
    26161 gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga
    26221 aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga
    26281 ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca
    26341 tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa
    26401 gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc
    26461 gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa
    26521 catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt
    26581 tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt
    26641 atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc
    26701 gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg
    26761 tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat
    26821 tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg
    26881 ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca
    26941 acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg
    27001 atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa
    27061 tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa
    27121 aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag
    27181 tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct
    27241 ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct
    27301 ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa
    27361 tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg
    27421 aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa
    27481 ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt
    27541 gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat acaggattcg
    27601 ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta
    27661 gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat
    27721 catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt
    27781 cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca
    27841 gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt
    27901 gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct
    27961 gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt
    28021 ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat
    28081 agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga
    28141 caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt
    28201 gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat
    28261 ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa
    28321 tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc
    28381 aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct
    28441 tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat
    28501 tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc
    28561 cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca
    28621 ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata
    28681 aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt
    28741 caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt
    28801 gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta
    28861 tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg
    28921 ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa
    28981 gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg
    29041 gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag
    29101 taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg
    29161 tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc
    29221 tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt
    29281 tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta
    29341 aaaataacaa tgataatata at
//
LOCUS       DADVXN010000024        29250 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.025, whole genome shotgun sequence.
ACCESSION   DADVXN010000024 DADVXN010000000
VERSION     DADVXN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.025"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1031)
                     /locus_tag="KB942_000938"
     CDS             complement(<1..1031)
                     /locus_tag="KB942_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6851384.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="KB942_000939"
     CDS             complement(1113..1802)
                     /locus_tag="KB942_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6851385.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB942_000940"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB942_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBB6851386.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="KB942_000941"
     CDS             complement(2329..2832)
                     /locus_tag="KB942_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBB6851387.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB942_000942"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB942_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBB6851388.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB942_000943"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB942_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBB6851389.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB942_000944"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB942_000944"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBB6851390.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB942_000945"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB942_000945"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBB6851391.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="KB942_000946"
     CDS             complement(6444..7463)
                     /locus_tag="KB942_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6851392.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="KB942_000947"
     CDS             complement(7636..8541)
                     /locus_tag="KB942_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6851393.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB942_000948"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB942_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBB6851394.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB942_000949"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB942_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBB6851395.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB942_000950"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB942_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6851396.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="KB942_000951"
     CDS             9754..10398
                     /locus_tag="KB942_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6851397.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="KB942_000952"
     CDS             complement(10454..10957)
                     /locus_tag="KB942_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6851398.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="KB942_000953"
     CDS             11554..12519
                     /locus_tag="KB942_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6851399.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="KB942_000954"
     CDS             complement(12618..13790)
                     /locus_tag="KB942_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851400.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="KB942_000955"
     CDS             complement(13777..14922)
                     /locus_tag="KB942_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851401.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="KB942_000956"
     CDS             complement(14936..15868)
                     /locus_tag="KB942_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851402.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="KB942_000957"
     CDS             15985..17064
                     /locus_tag="KB942_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6851403.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="KB942_000958"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="KB942_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="KB942_000959"
     CDS             complement(17789..18016)
                     /locus_tag="KB942_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6851404.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="KB942_000960"
     CDS             complement(18119..20707)
                     /locus_tag="KB942_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBB6851405.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="KB942_000961"
     CDS             complement(20727..21386)
                     /locus_tag="KB942_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6851406.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB942_000962"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB942_000962"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBB6851407.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="KB942_000963"
     CDS             complement(22159..23025)
                     /locus_tag="KB942_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6851408.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="KB942_000964"
     CDS             23134..23775
                     /locus_tag="KB942_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBB6851409.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB942_000965"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB942_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBB6851410.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="KB942_000966"
     CDS             complement(25216..26115)
                     /locus_tag="KB942_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6851411.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="KB942_000967"
     CDS             complement(26117..26914)
                     /locus_tag="KB942_000967"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBB6851412.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="KB942_000968"
     CDS             complement(26892..27596)
                     /locus_tag="KB942_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBB6851413.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="KB942_000969"
     CDS             27727..28302
                     /locus_tag="KB942_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBB6851414.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="KB942_000970"
     CDS             28420..29097
                     /locus_tag="KB942_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6851415.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8615 a         6183 c         5262 g         9190 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataatttgg acagaacaga
    29161 aagaagaaaa cctctttctg ttctgtttat ttggttaaaa atgttttgcc atgacgaatc
    29221 ataattcgta gtttgaaatt gtaaaagttg
//
LOCUS       DADVXN010000025        28135 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.026, whole genome shotgun sequence.
ACCESSION   DADVXN010000025 DADVXN010000000
VERSION     DADVXN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.026"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(96..575)
                     /locus_tag="KB942_000971"
     CDS             complement(96..575)
                     /locus_tag="KB942_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6851416.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(648..932)
                     /locus_tag="KB942_000972"
     CDS             complement(648..932)
                     /locus_tag="KB942_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851417.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(1010..1204)
                     /gene="ssrS"
                     /locus_tag="KB942_000973"
     ncRNA           complement(1010..1204)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KB942_000973"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1276..2562)
                     /locus_tag="KB942_000974"
     CDS             complement(1276..2562)
                     /locus_tag="KB942_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBB6851418.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2700..3371
                     /locus_tag="KB942_000975"
     CDS             2700..3371
                     /locus_tag="KB942_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBB6851419.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3337..3825
                     /locus_tag="KB942_000976"
     CDS             3337..3825
                     /locus_tag="KB942_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBB6851420.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            3838..4785
                     /gene="prmA"
                     /locus_tag="KB942_000977"
     CDS             3838..4785
                     /gene="prmA"
                     /locus_tag="KB942_000977"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBB6851421.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            4786..5550
                     /locus_tag="KB942_000978"
     CDS             4786..5550
                     /locus_tag="KB942_000978"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBB6851422.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            5783..7996
                     /locus_tag="KB942_000979"
     CDS             5783..7996
                     /locus_tag="KB942_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBB6851423.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            8007..8453
                     /locus_tag="KB942_000980"
     CDS             8007..8453
                     /locus_tag="KB942_000980"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBB6851424.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            8624..9883
                     /locus_tag="KB942_000981"
     CDS             8624..9883
                     /locus_tag="KB942_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBB6851425.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9972..10919)
                     /locus_tag="KB942_000982"
     CDS             complement(9972..10919)
                     /locus_tag="KB942_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6851426.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            11313..12605
                     /locus_tag="KB942_000983"
     CDS             11313..12605
                     /locus_tag="KB942_000983"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBB6851427.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            12620..14389
                     /gene="aspS"
                     /locus_tag="KB942_000984"
     CDS             12620..14389
                     /gene="aspS"
                     /locus_tag="KB942_000984"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBB6851428.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            14498..15373
                     /locus_tag="KB942_000985"
     CDS             14498..15373
                     /locus_tag="KB942_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6851429.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            15639..17522
                     /locus_tag="KB942_000986"
     CDS             15639..17522
                     /locus_tag="KB942_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6851430.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(17597..17881)
                     /locus_tag="KB942_000987"
     CDS             complement(17597..17881)
                     /locus_tag="KB942_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6851431.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(17871..18605)
                     /locus_tag="KB942_000988"
     CDS             complement(17871..18605)
                     /locus_tag="KB942_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBB6851432.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            18734..19357
                     /locus_tag="KB942_000989"
     CDS             18734..19357
                     /locus_tag="KB942_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBB6851433.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            19549..20670
                     /locus_tag="KB942_000990"
     CDS             19549..20670
                     /locus_tag="KB942_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBB6851434.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            20677..23805
                     /locus_tag="KB942_000991"
     CDS             20677..23805
                     /locus_tag="KB942_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBB6851435.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            23824..27018
                     /locus_tag="KB942_000992"
     CDS             23824..27018
                     /locus_tag="KB942_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBB6851436.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(27164..27871)
                     /locus_tag="KB942_000993"
     CDS             complement(27164..27871)
                     /locus_tag="KB942_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6851437.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9371 a         5020 c         5989 g         7755 t
ORIGIN      
        1 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
       61 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
      121 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
      181 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
      241 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
      301 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
      361 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
      421 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
      481 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
      541 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
      601 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
      661 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
      721 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
      781 aaaacagcat atgcagctcc gtaaaattgt aggaatcctt gatttttatc atttttgttg
      841 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
      901 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
      961 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
     1021 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
     1081 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
     1141 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
     1201 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
     1261 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
     1321 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
     1381 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
     1441 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
     1501 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
     1561 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
     1621 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
     1681 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
     1741 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
     1801 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
     1861 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
     1921 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
     1981 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
     2041 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
     2101 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
     2161 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
     2221 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
     2281 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
     2341 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
     2401 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
     2461 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
     2521 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
     2581 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
     2641 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
     2701 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
     2761 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
     2821 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
     2881 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
     2941 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
     3001 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
     3061 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
     3121 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
     3181 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
     3241 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
     3301 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
     3361 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
     3421 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
     3481 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
     3541 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
     3601 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
     3661 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
     3721 gaagctgtat tcgagcttca ctgggataat cagcaactag cagaattagt agaagtaaaa
     3781 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
     3841 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
     3901 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
     3961 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
     4021 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
     4081 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
     4141 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
     4201 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
     4261 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
     4321 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
     4381 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
     4441 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
     4501 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
     4561 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
     4621 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
     4681 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
     4741 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
     4801 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
     4861 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
     4921 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
     4981 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
     5041 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
     5101 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
     5161 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
     5221 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
     5281 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
     5341 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
     5401 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
     5461 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
     5521 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
     5581 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
     5641 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
     5701 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
     5761 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
     5821 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
     5881 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
     5941 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
     6001 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
     6061 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
     6121 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
     6181 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
     6241 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
     6301 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
     6361 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
     6421 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
     6481 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
     6541 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
     6601 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
     6661 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
     6721 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
     6781 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
     6841 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
     6901 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
     6961 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
     7021 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
     7081 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
     7141 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
     7201 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
     7261 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
     7321 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
     7381 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
     7441 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
     7501 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
     7561 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
     7621 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
     7681 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
     7741 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
     7801 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
     7861 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
     7921 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
     7981 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
     8041 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
     8101 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
     8161 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
     8221 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
     8281 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
     8341 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
     8401 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
     8461 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
     8521 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
     8581 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
     8641 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
     8701 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
     8761 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
     8821 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
     8881 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
     8941 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
     9001 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
     9061 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
     9121 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
     9181 aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
     9241 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
     9301 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
     9361 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
     9421 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
     9481 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
     9541 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
     9601 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
     9661 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
     9721 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
     9781 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
     9841 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
     9901 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
     9961 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    10021 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    10081 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    10141 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    10201 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    10261 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    10321 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    10381 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    10441 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    10501 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    10561 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    10621 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    10681 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    10741 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    10801 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    10861 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    10921 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    10981 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    11041 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    11101 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    11161 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    11221 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    11281 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    11341 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    11401 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    11461 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    11521 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    11581 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    11641 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    11701 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    11761 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    11821 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    11881 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    11941 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    12001 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    12061 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    12121 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    12181 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    12241 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    12301 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    12361 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    12421 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    12481 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    12541 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    12601 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    12661 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    12721 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    12781 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    12841 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    12901 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    12961 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    13021 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    13081 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    13141 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    13201 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    13261 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    13321 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    13381 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    13441 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    13501 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    13561 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    13621 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    13681 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    13741 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    13801 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    13861 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    13921 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    13981 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    14041 ttatgatatc gtcttgaacg gttatgaact aggcggaggt tctatccgta ttcatactcg
    14101 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    14161 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    14221 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    14281 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    14341 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    14401 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    14461 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    14521 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    14581 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    14641 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    14701 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    14761 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    14821 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    14881 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    14941 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    15001 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    15061 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    15121 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    15181 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    15241 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    15301 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    15361 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    15421 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    15481 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    15541 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    15601 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    15661 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    15721 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    15781 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    15841 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    15901 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    15961 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    16021 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    16081 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    16141 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    16201 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    16261 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    16321 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    16381 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    16441 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    16501 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    16561 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    16621 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    16681 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    16741 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    16801 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    16861 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    16921 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    16981 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    17041 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    17101 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    17161 aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca ttctcggaag
    17221 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    17281 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    17341 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    17401 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    17461 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    17521 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    17581 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    17641 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    17701 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    17761 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    17821 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    17881 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    17941 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    18001 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    18061 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    18121 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    18181 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    18241 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    18301 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    18361 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    18421 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    18481 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    18541 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    18601 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    18661 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    18721 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    18781 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    18841 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    18901 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    18961 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    19021 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    19081 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    19141 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    19201 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    19261 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    19321 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    19381 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    19441 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    19501 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    19561 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    19621 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    19681 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    19741 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    19801 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    19861 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    19921 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    19981 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    20041 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    20101 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    20161 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    20221 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    20281 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    20341 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    20401 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    20461 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    20521 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    20581 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    20641 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    20701 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    20761 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    20821 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    20881 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    20941 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    21001 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    21061 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    21121 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    21181 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    21241 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    21301 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    21361 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    21421 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    21481 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    21541 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    21601 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    21661 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    21721 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    21781 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    21841 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    21901 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    21961 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    22021 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    22081 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    22141 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    22201 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    22261 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    22321 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    22381 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    22441 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    22501 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    22561 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    22621 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    22681 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    22741 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    22801 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    22861 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    22921 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    22981 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    23041 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    23101 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    23161 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    23221 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    23281 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    23341 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    23401 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    23461 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    23521 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    23581 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    23641 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    23701 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    23761 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    23821 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    23881 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    23941 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    24001 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    24061 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    24121 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    24181 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    24241 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    24301 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    24361 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    24421 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    24481 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    24541 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    24601 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    24661 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    24721 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    24781 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    24841 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    24901 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    24961 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg cgaagtcatc
    25021 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    25081 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    25141 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    25201 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    25261 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    25321 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    25381 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    25441 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    25501 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    25561 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    25621 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    25681 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    25741 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    25801 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    25861 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    25921 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    25981 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    26041 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    26101 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    26161 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    26221 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    26281 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    26341 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    26401 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    26461 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    26521 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    26581 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    26641 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    26701 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    26761 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    26821 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    26881 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    26941 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    27001 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    27061 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    27121 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    27181 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    27241 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    27301 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    27361 ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    27421 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    27481 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    27541 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    27601 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    27661 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    27721 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    27781 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    27841 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    27901 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    27961 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    28021 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    28081 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttat
//
LOCUS       DADVXN010000026        27924 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.027, whole genome shotgun sequence.
ACCESSION   DADVXN010000026 DADVXN010000000
VERSION     DADVXN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.027"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(462..2354)
                     /locus_tag="KB942_000994"
     CDS             complement(462..2354)
                     /locus_tag="KB942_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851438.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2351..4078)
                     /locus_tag="KB942_000995"
     CDS             complement(2351..4078)
                     /locus_tag="KB942_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851439.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4094..4744)
                     /locus_tag="KB942_000996"
     CDS             complement(4094..4744)
                     /locus_tag="KB942_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6851440.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4948..5244
                     /locus_tag="KB942_000997"
     CDS             4948..5244
                     /locus_tag="KB942_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6851441.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5436..6056
                     /locus_tag="KB942_000998"
     CDS             5436..6056
                     /locus_tag="KB942_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBB6851442.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6148..7314)
                     /locus_tag="KB942_000999"
     CDS             complement(6148..7314)
                     /locus_tag="KB942_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6851443.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7326..7679)
                     /locus_tag="KB942_001000"
     CDS             complement(7326..7679)
                     /locus_tag="KB942_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6851444.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7807..8829
                     /locus_tag="KB942_001001"
     CDS             7807..8829
                     /locus_tag="KB942_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBB6851445.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8848..9009
                     /locus_tag="KB942_001002"
     CDS             8848..9009
                     /locus_tag="KB942_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851446.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9268..9702)
                     /locus_tag="KB942_001003"
     CDS             complement(9268..9702)
                     /locus_tag="KB942_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851447.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9812..11671)
                     /locus_tag="KB942_001004"
     CDS             complement(9812..11671)
                     /locus_tag="KB942_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6851448.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11789..12949)
                     /locus_tag="KB942_001005"
     CDS             complement(11789..12949)
                     /locus_tag="KB942_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBB6851449.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13256..14680)
                     /gene="araA"
                     /locus_tag="KB942_001006"
     CDS             complement(13256..14680)
                     /gene="araA"
                     /locus_tag="KB942_001006"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBB6851450.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14696..15394)
                     /locus_tag="KB942_001007"
     CDS             complement(14696..15394)
                     /locus_tag="KB942_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6851451.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15351..17012)
                     /locus_tag="KB942_001008"
     CDS             complement(15351..17012)
                     /locus_tag="KB942_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBB6851452.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17230..18630
                     /locus_tag="KB942_001009"
     CDS             17230..18630
                     /locus_tag="KB942_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBB6851453.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18882..19961)
                     /locus_tag="KB942_001010"
     CDS             complement(18882..19961)
                     /locus_tag="KB942_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6851454.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20047..21006)
                     /locus_tag="KB942_001011"
     CDS             complement(20047..21006)
                     /locus_tag="KB942_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6851455.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21018..22529)
                     /locus_tag="KB942_001012"
     CDS             complement(21018..22529)
                     /locus_tag="KB942_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6851456.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22551..23114)
                     /locus_tag="KB942_001013"
     CDS             complement(22551..23114)
                     /locus_tag="KB942_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6851457.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23120..23983)
                     /locus_tag="KB942_001014"
     CDS             complement(23120..23983)
                     /locus_tag="KB942_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851458.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23997..24893)
                     /locus_tag="KB942_001015"
     CDS             complement(23997..24893)
                     /locus_tag="KB942_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851459.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25068..26357)
                     /locus_tag="KB942_001016"
     CDS             complement(25068..26357)
                     /locus_tag="KB942_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6851460.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26329..27846)
                     /locus_tag="KB942_001017"
     CDS             complement(26329..27846)
                     /locus_tag="KB942_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6851461.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8157 a         5875 c         4847 g         9045 t
ORIGIN      
        1 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aaaagtcgct tagtttcgag
       61 tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt ggcttaatac
      121 cgagaaactg gcttttgaac accgtttacc aagaggttgt gagcctgccg tttaactccg
      181 agtcacaagg agcaatttca aaaaacagct tctcaggttt gttgactatt gtgacttgtg
      241 accgaggagt tggcaggaga acaccgttta aggaagaggt tgtaaaatca gcggtttgac
      301 ctgagtatta gagatacaaa acggaaaaat agcttctcat attttgccgt tttttgagct
      361 aataccgaag gacagctgat tgaacaccgt ttattcggaa aaagggactg tgacacgact
      421 tttgtcacag cccctaaaaa aaataataaa tttgtactat tctatgaagc ttcttcttca
      481 gaaaactgac tgttataaag atctgcatag aatccgtctt tttccatcaa tgtatcgtgg
      541 tttcctgttt ctacgatcga accatgattc atcacaataa tgttgtctgc atcgcgtatt
      601 gtagacaagc ggtgagcaac aacgaaactt gtccggtttt ccaataggcg gttcatcgct
      661 ttttgaatca gtatttctgt tcgtgtatct acactagatg ttgcttcatc aaggatcagg
      721 acatctggat ttgctaagaa tgcccgcgca attgtgatta gctgacgctg cccttgcgaa
      781 atattgcttg catcctcgtt taatacagtg ttatatcctt ctggcaattt acgaacaaaa
      841 tcgtctacgt gagcagcttt agcagcctca atgatttgtt cgtctgttgc atcgtcgttc
      901 ccatatttga tattgtcata gatacttcct gtaaacagcc aagtatcttg cagtaccatt
      961 gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg tatctacacc atcgtaacga
     1021 atactacctc cagaaacatc gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct
     1081 gcaccagtcg gacctacaat agcgaccatc tcaccaggtt tgacatccaa gttaaaatca
     1141 gtcattagca agttatctgg tgtgtagccg aattgaacat tttcaaaacg aactttgtat
     1201 ggtgtatctt tttcttctgg aaggccagat ttttcattgg acatctcttc ttcatccaac
     1261 acttcaaata cccgttcggc tgaagcaact gttgattgga tagtgttcat caaacttgca
     1321 agttgagtga ttggttgaga gaactgtgtt gtatattgca agaacgcttg aacatcccct
     1381 aatgtcatgt tcccatttgc aacttttact ccgcctagta cagcgacgaa cacatagcct
     1441 agatttttaa cgaaattcat caacggcatg atgattgccg aaataaattg agctttccaa
     1501 ccagcggcat acagattctg attttctttt tcaaatactt cctggtcttt ttcagtatga
     1561 ttgaatgttt taacaatggt atgaccacca tatgtttctt ctacttggtt attcaataaa
     1621 cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg
     1681 atgatcaagc ttaaagggat catcaataac gcgactaacg tcaattgcca gctgattgaa
     1741 agcatcatca cgatgacccc aatcaatgtg actgcactag ttacaaattg agtcaaattt
     1801 tgctgcagcg tgcttgcgat gttatccata tcattgattg ctcgagacat gatatcccca
     1861 tttgtatgtg tatcataata ggaaatcggt acacgattca ttttggcttc caaatcatga
     1921 cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa taaattgctg taaaaagttg
     1981 aatacagcag aaatcaaata catcacgatt actgtcgcaa tgatctgcgc gattttatca
     2041 aagtctatcg gaaattttgt tacttgttgg ccttgttgca tctgttgagc gccagccatt
     2101 acacctttga atatttctgt ggttgctttc cccaagacct ttggtgtttg gatttggaag
     2161 atcacggctg ctattgctag tacaaataca gcgatgatcg caaccatacg tttagacata
     2221 tagccaaaca atcggcgtac cgttccccag aaatttttag gcttttcacc ttttacaccg
     2281 agatttctgc caggtccacc atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg
     2341 tttgttttac tcatgctaag tcctcctctc ttagttgtga atgaacgatt tcttgataag
     2401 tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta
     2461 gcacaaggat catatctgca tccattactg tacttactcg ttgagcaact aaaacggtga
     2521 tactttcttt catattcgtt ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca
     2581 acgctgagaa ggaatcatca aacacataga tatctgctgt tttgactaat gcccgtgcaa
     2641 ttgccagacg ctgtctttgt ccaccagaga agtttcctcc tccttgttcc acatggctat
     2701 ctaagccatc ttccatctct gaaacaaaat ctttagcttg tgcgatttct aatgctttcc
     2761 aaatcatttc atctgttgca tccggcttac catactgcat attgtctcgg atcgttccag
     2821 agaacagaac tgctttttga ggaacaaagc ccattaattc tcgcaaatta tattgagtca
     2881 tgtcactaat gtccgttcca tttatttgga tcgaaccaga ttcgatgtca tataatcgag
     2941 ggatcaaatt gactaaggtc gttttacctg aaccagtccc ccctatgatt gcgacgatct
     3001 cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag tttttctgct ccatgatatc
     3061 gataattgac atgattgaag gccaatgttg cgttttttcc agcaaaagaa actgttttag
     3121 gattttcagg gtcatggata ccgatttttt catccaacac ttcattaata cggtcagctg
     3181 aagcttgtgc acgaggcacc atgataaaaa tagcggaaag catcataaag ctgatcaaga
     3241 tctgcatcgc ataagtcata aatgcaatca gattaccgac ttccaaagtc atatctgcta
     3301 tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc gctcatgatc aatgtcatca
     3361 ttggcatcat caatgcgacg atcgtattaa ctttgattgc cgtatttgta taatctttat
     3421 tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc gaatgctcga atcactctga
     3481 cccctgtcaa tccttctcga aataccaggt ttaaacggtc agttttcttt tgcatacttt
     3541 tgaataatgg aacagcaaag tacatgattc cgccgatcaa aataatcatt actgggatga
     3601 caactaagaa gatttttgtc aactgatgat ctttttgata agcaagtatg ctcgcgccaa
     3661 tcaacgtgat tggtgcattg atcatcatac gtaaaaacat ttgtgtaacc atttggattt
     3721 gagtcacatc attggtcgtt cttgtgatca aagaggccgt tccatattta tcaaacgcat
     3781 cttttgtcag actctctgat tttttataga catccgtacg caattgctta cccagtttct
     3841 gtgattcacg agtagcaaaa aatgtatttg cgatagcagc taagatactg atcaaagaaa
     3901 agccgatcat cacaaaacct gtatgccaaa tataatcgac atctccttgc gctacccctt
     3961 tatcaataat attggaggta agtgtcggca aatataaatc tgccagtact tggatcacca
     4021 tgaaaactgc agctgcaatt gctgaagtca aggagatccg ttttacgatt tttagcatct
     4081 agttcccttc tttctattta tatactccgt ttttcagcca agaaagtttc aaattgaatt
     4141 cactgattgc ttgctctact gagtaatctt cagatgttga aaataagcga tacgaatgcg
     4201 caattgttgt aaatacagta ttcatcacta attggatcag taattccaat tcatgatcat
     4261 tttttacttt cagcttcgtc cgatcgatca attcaagcaa ctcttttgca tcttgtttgt
     4321 gttctttatg tggatcgtga ggcgtttttt ggcgactcgt atgaggagcg acttttcttg
     4381 aggcacggta atccatgttc ataaacaagt tacgataaaa agaagcattc tcgccttcca
     4441 aaatctcata aatcatttta gaaaaatagg attcgaatcc tttgaataga tcaccatcgg
     4501 attctttcat tagttgtaac atatctttag agctgtcacg tctcattgtc tcaaagtaat
     4561 aaaaataaag atcctcttta tcttcaaaat actgatagaa actgcctcta gggatatccg
     4621 ctaatttgac gatattagca atcgaagctt cttttaaggg tactctagaa aactcgattc
     4681 tcgcagcctc gattagtctt ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg
     4741 gcatatggat tctcctttct ttcaagatga cacattgtca tatgacgcta tgtcattata
     4801 acacctccta cctgttttgc aagacatttg catagttttt ttcattatca tccgatacat
     4861 taaaaaagtg ataagaggaa tccttccatt tgaattaatg agagttgccc attaaaatag
     4921 ccacatacta aacaaaggat gagataaatg gcccaatcaa ttagtgtcca agacttcaat
     4981 aaattgcctc tcgatgctga cagcatcgtt ttagatacaa gaaatacctt agattacaca
     5041 aaaaaccact taagccactc gctttctatc cccgctgcta tcttgccaag acaattaaaa
     5101 aatctagatc caaagaaact ttattatatt ctaagctata caggcaggcg atcagaagca
     5161 ttagccgatc aacttactgc taatggtttt cgtgctgttt atatcattgg tgggatggaa
     5221 gctcttcagt cgagtgttgc ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa
     5281 aaatcgtgtc acagcctctt ttataccctg tcttttttta gttatttatt cttcaacctt
     5341 atcaatatga cttgatagcc ttttcttttt cgagtaaact gattttgtta gtcatagtta
     5401 taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta gtagtcaaag
     5461 gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct tttttaacaa
     5521 gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat cgcgatgata
     5581 ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct ggtgcagaat
     5641 tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa ttgactgatc
     5701 agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta aatatcccaa
     5761 caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc cgctatacag
     5821 aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa tctaatggcg
     5881 gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta aactttgtag
     5941 gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct gaccgtgcag
     6001 atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca ttttaaaaat
     6061 tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca tttgtgcctt
     6121 ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg gattttcttc
     6181 ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta ctttagctgc
     6241 tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc cagaagtcag
     6301 cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta ctaatggaga
     6361 gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt tgatgatcat
     6421 gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa atgatcctaa
     6481 gtaagggatc aggtttaaaa aacccgcaat aatccccaac gtgacagcat actctaaacc
     6541 aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag ttagttgtcc
     6601 tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg tcggtttttg
     6661 catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt ctttcaatag
     6721 ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta ccgcgccgac
     6781 aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat cgcctgccga
     6841 attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga acagcgggtc
     6901 ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga agttactgat
     6961 gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc atgcgattag
     7021 tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt ttcgcttttc
     7081 aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa tgataggcag
     7141 acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa
     7201 aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt gattatttaa
     7261 aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt ccataattcc
     7321 tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct atgcggatga
     7381 agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat caatgatacg
     7441 tgagctactg tctttaaatt cccattggcc atcggaccga cataatcgat cgtatatacc
     7501 tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact
     7561 tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc
     7621 atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc ctgaatcatt
     7681 gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc tttagccgtg
     7741 tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta ggaggaatca
     7801 gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag taaaggaatc
     7861 tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt agctgttgaa
     7921 gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac agttactagt
     7981 gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt gaatgcggta
     8041 acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca attactatac
     8101 ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga tggcgaactg
     8161 aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat
     8221 gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg tgatcttggt
     8281 acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct tgtaactacc
     8341 ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa tggacaattc
     8401 gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa taaagaaaca
     8461 ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga cggtgaaaat
     8521 ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc taaccgagga
     8581 cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca aaacaagcaa
     8641 atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag caataatttt
     8701 gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca tcctgaaaca
     8761 ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt
     8821 gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga cagactacgg
     8881 gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta tagcgaagag
     8941 gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt acagaatgta
     9001 gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac ttttccgtct
     9061 atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc gacggaaaag
     9121 gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt atgtcattat
     9181 cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt agataactct
     9241 agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag tcattttttg
     9301 gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag
     9361 atcttcatct acccctgatt caagatcgat actttggatc atttcttcac agcctgctac
     9421 atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg ctggtggacg
     9481 caaagtactg actttcataa gcattgcccg atattttttt gttccttctt caaataacag
     9541 gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat caattgcgtt
     9601 tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt ccttgacgat
     9661 ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt tcctactttc
     9721 tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag tacttgaaaa
     9781 aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta
     9841 aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg tcgtaaatct
     9901 cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga tagtacagtt
     9961 ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa aagccatttg
    10021 ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg gtgattgcat
    10081 cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat tgtgtttcat
    10141 gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct ggaagatgat
    10201 ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc gtgaactgct
    10261 tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca agtgtcccac
    10321 ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg tagggcatat
    10381 gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg agaagtgtca
    10441 ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc gtatccgtat
    10501 aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa acatcctctc
    10561 ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta
    10621 acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg taaggggtgg
    10681 tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg
    10741 ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga tcagctactt
    10801 cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga ctaggatctg
    10861 agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca gttttatttt
    10921 tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct ttcgaatatc
    10981 cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg ataaaaccag
    11041 tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta ttatatgctt
    11101 ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt ccaattgcca
    11161 caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg cttcgaaaca
    11221 tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact gtaaacagca
    11281 tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa tcgtctggat
    11341 aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat acaccgatgg
    11401 ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc agaaaaataa
    11461 gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa gaaaaagcaa
    11521 atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat aaattactaa
    11581 aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt
    11641 tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc tatcaataaa
    11701 agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga
    11761 attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt aataagcgga
    11821 cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc tgttcaaaat
    11881 cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta cgcgatatat
    11941 ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat atattggttt
    12001 tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt gcggatggat
    12061 aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct tcaactgcca
    12121 aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc aacataagta
    12181 tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta gccagatgtt
    12241 gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt cctacattga
    12301 taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat ccttttgtat
    12361 cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact gtttctaaat
    12421 ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag acatccatcc
    12481 ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca agaagaccga
    12541 tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct gcctgttcta
    12601 aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc ggacgggaag
    12661 ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca cctggtgtat
    12721 tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc accccaacac
    12781 cagctcttga aattgctagc aaatctggtg taagccattc atctttcact ttcgtactac
    12841 gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc ccttgtaact
    12901 tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata aaaccccctc
    12961 cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat ttgacttcct
    13021 agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag gctatgacaa
    13081 gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata tcttgataaa
    13141 tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt gaaaagcaaa
    13201 tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc tctttttatc
    13261 taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga tctgttgtta
    13321 gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa tggacacctt
    13381 catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta ggtgcttcag
    13441 tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta ccgaaatctg
    13501 ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc gccggatctt
    13561 ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg
    13621 gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct agctcgtaag
    13681 tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc aaccgatcaa
    13741 tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc atcaaacgtt
    13801 gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag
    13861 cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga gcttgtactt
    13921 ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta tacaattcct
    13981 tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt acttgtacaa
    14041 gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct tccactttgt
    14101 ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact tttattccaa
    14161 aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt tggatctcag
    14221 gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca ttgatgaacc
    14281 catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata tcgatcgttt
    14341 tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt tgtagaagct
    14401 ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg atcacacctg
    14461 ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca gcagtaactg
    14521 ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt tcatcgacca
    14581 tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa tgctgtgatc
    14641 ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac tccttttatt
    14701 tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta tctaaaatcc
    14761 gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc atcttgcata
    14821 cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc caagtaaatg
    14881 gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg cgttctttga
    14941 atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt tcctgttgtg
    15001 tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc gtccccatcg
    15061 caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga gtatggacga
    15121 ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta tccgaagagg
    15181 gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg tcgtctgctg
    15241 tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt tcaggatcaa
    15301 aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag tcaagatttg
    15361 cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt ttcttgcaat
    15421 tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat aaatatctga
    15481 tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg tccaaacacg
    15541 tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata ggctgctaat
    15601 aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac tggtgcctcc
    15661 atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc cccatgcccc
    15721 actaaacgat caatttggac attctcagat ttcaatatat ccattccgat tctcattgca
    15781 ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg gctacctggt
    15841 gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc ggaaaaataa
    15901 ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt gaataatacc
    15961 gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc ttcaaaaata
    16021 ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc tactagttct
    16081 cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc tttttctaat
    16141 acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtcccttt ggctacactg
    16201 ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg gattcctggt
    16261 ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt gccagcttct
    16321 tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac gtgcgctttt
    16381 tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt tgaaggatcg
    16441 ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt aagctgccaa
    16501 tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc ttctttattc
    16561 aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta ttgaaatttt
    16621 tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg aaatggtact
    16681 agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact gaacccaatc
    16741 gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc catttttcgg
    16801 taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat accattttct
    16861 agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga atcaatcaat
    16921 acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc atctatgaca
    16981 tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc ttctcgtcaa
    17041 acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact tttgtacgta
    17101 catgtcaagc agttatttac aagaatacaa taaaaaacac ttgttttgta cgtacataag
    17161 ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt ctagaaggag
    17221 agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt ttttggcgca
    17281 tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt acctttttta
    17341 cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac gtcttcggtc
    17401 atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa gcttggacga
    17461 cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct ttctgcatct
    17521 gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg tttagctgtt
    17581 ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc tagactaagg
    17641 ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct ttcctatgtt
    17701 gcagattatt tgctaaaagg gttgcctgaa acgatggctt ggcgcgtgat gttagggctc
    17761 gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga gtcaccgcgt
    17821 tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta tattcggaca
    17881 ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca agaaaagaca
    17941 accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt cattgccggt
    18001 attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta ctatattccg
    18061 ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg gccgatcatc
    18121 caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga caagttcaat
    18181 cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttctttcat cctaccagct
    18241 gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt gtttttatgt
    18301 atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat cgtaggcgaa
    18361 atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt taattggatt
    18421 ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc tcaagaaatc
    18481 gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca agagatcgta
    18541 ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa aaaaacttat
    18601 cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg tcagcttcaa
    18661 gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa aacagcttct
    18721 catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt ttagtggaga
    18781 ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa cattgcgacg
    18841 agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc gttttctttc
    18901 actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc ctgtcctttt
    18961 tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt tggatgcgtc
    19021 aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt tcctacgatc
    19081 gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat taaactagct
    19141 atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa ccgttgtact
    19201 gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc ttccgtcaaa
    19261 aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta ttttccttga
    19321 agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat caaatattct
    19381 gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt taactcttca
    19441 taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac ataaagtgct
    19501 aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc taccccataa
    19561 gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt actggctaaa
    19621 agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat cgacggaaaa
    19681 atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt caatggtgtt
    19741 ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt acgcagatag
    19801 ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac gtcatatatt
    19861 tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc acgaaggatc
    19921 ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc ccctttttat
    19981 ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa tcaaggtttc
    20041 ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt gctcattcca
    20101 cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc ctttttggtt
    20161 cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg aaaccgtaaa
    20221 cgagttgtga ccaggaccaa attgcttatt ttttttagaa gaaacgaata ctggctccga
    20281 taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc acagcaggcc
    20341 catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt tcccattgcg
    20401 agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt catactcagg
    20461 tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt cggatagata
    20521 aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat atagctttcc
    20581 ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt ttatttgtcc
    20641 cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta cgaacatgcg
    20701 gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt aaatatacca
    20761 ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg gtccttcttc
    20821 atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg actttgccct
    20881 tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat aaccatctgt
    20941 atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat aaagtgtctt
    21001 catcataaca aatttcctca cattttcttt attctaatta cattccaaga gtatttttct
    21061 atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc aggttttatt
    21121 ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa tgtgtagtgc
    21181 tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg ttcatttgtg
    21241 cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact gtccacataa
    21301 ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg tttcaatact
    21361 tgacctttag catatttcga taagtgttgc atcacataat aagttggatt cctccatgct
    21421 tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga ttgacaacta
    21481 atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa taataatgta
    21541 tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc cctatgtctt
    21601 ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt tttctcactt
    21661 ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg acgatcgaat
    21721 cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg aggtttagtc
    21781 aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc tacattctca
    21841 taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg tggcgtagag
    21901 cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt tgaggcttca
    21961 tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg accgtctagc
    22021 tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt ttttattctt
    22081 aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt ttttgcttca
    22141 tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac atgactgatc
    22201 catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca tgctaaatca
    22261 attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata accagatgta
    22321 aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata agagaggaca
    22381 tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat ccctgtatag
    22441 acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat ctctcctacc
    22501 gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc ctacctttca
    22561 tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa gataaaaata
    22621 aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca acaaagaata
    22681 tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg taaaaatgtg
    22741 atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga ataaataagt
    22801 aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa ttgattaaca
    22861 agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa atgttgcttt
    22921 attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca ttgcgccatt
    22981 acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca cacgattacc
    23041 cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata aagtttagtc
    23101 tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct tgaacaaaat
    23161 atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc gttccagcca
    23221 ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct gctggtaaag
    23281 ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc caactattca
    23341 tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac atgattttca
    23401 aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta
    23461 aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata cgaggaatga
    23521 tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat agaggaatca
    23581 tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa actgattttc
    23641 ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat acagaagtga
    23701 tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa gggatcgttg
    23761 tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta ggcggaaatg
    23821 agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa
    23881 taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca ctaactgctt
    23941 ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt tccctcctat
    24001 ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat agttgcaaca
    24061 atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg tgctgtaaat
    24121 cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc accagcggtt
    24181 gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa aatacaaaaa
    24241 cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg atttgttgca
    24301 ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc agtcaagaga
    24361 atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt cggcatagca
    24421 agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa caagtaagga
    24481 atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa
    24541 caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt atttttagat
    24601 aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag tacggtgaaa
    24661 tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg tgtgaaattt
    24721 tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa actaatagca
    24781 aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga tggagttaaa
    24841 aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt catatagtcc
    24901 acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct gaataagata
    24961 tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag gtcgatcatt
    25021 cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta tttttgcagt
    25081 aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc tcctgctaat
    25141 aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg ggtaaaacct
    25201 ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat aggatcattt
    25261 tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt ttcgatactc
    25321 cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac agcttctttt
    25381 ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat agctgttttt
    25441 ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc gtttgctttc
    25501 aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc attcattaaa
    25561 ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg gatcattttt
    25621 tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt agtataatca
    25681 tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc agttccatcc
    25741 ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg tgccatttct
    25801 gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg tttaaacaaa
    25861 tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata ctgtttacct
    25921 tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac tccatcatat
    25981 tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc aaaatctgga
    26041 taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg aagtttttca
    26101 ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc atttgtttta
    26161 ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa cccattccaa
    26221 aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc accgcaagct
    26281 cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt acctttcatt
    26341 ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata gcggtttcaa
    26401 taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg gttttacttg
    26461 ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat
    26521 cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt cttctccacg
    26581 gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt ctaaatacgg
    26641 cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc ttaacacctc
    26701 tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc tccaagcttc
    26761 tccgtttgtt tctgtcctga ttggtgcaat aacatttacc aattgagcaa gacaagcaat
    26821 ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata gtaatgcatc
    26881 ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc
    26941 atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt tgatctgctt
    27001 cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag aaatgaaccg
    27061 atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt tgccataata
    27121 ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg ttaactccca
    27181 atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct caattgaatc
    27241 atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt cttccgctgt
    27301 tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg tcttaattcc
    27361 aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc ctttaggaaa
    27421 attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca gattgacagc
    27481 catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa tcccaacttc
    27541 attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg ctatgggccc
    27601 aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc taatcatggg
    27661 aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt tattagctga
    27721 tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt caataaatga
    27781 actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca acattgtggc
    27841 ttccataact gacctccttt aatttataca tgaataatac acctcgttga aagcgttgtc
    27901 aataatataa atgttaaact tact
//
LOCUS       DADVXN010000027        27921 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.028, whole genome shotgun sequence.
ACCESSION   DADVXN010000027 DADVXN010000000
VERSION     DADVXN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.028"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(386..1330)
                     /locus_tag="KB942_001018"
     CDS             complement(386..1330)
                     /locus_tag="KB942_001018"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6851462.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1626..2570)
                     /locus_tag="KB942_001019"
     CDS             complement(1626..2570)
                     /locus_tag="KB942_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851463.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2791..4005
                     /locus_tag="KB942_001020"
     CDS             2791..4005
                     /locus_tag="KB942_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6851464.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4027..6042
                     /locus_tag="KB942_001021"
     CDS             4027..6042
                     /locus_tag="KB942_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBB6851465.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6125..7498)
                     /locus_tag="KB942_001022"
     CDS             complement(6125..7498)
                     /locus_tag="KB942_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6851466.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7524..7808)
                     /locus_tag="KB942_001023"
     CDS             complement(7524..7808)
                     /locus_tag="KB942_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6851467.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7810..9825)
                     /locus_tag="KB942_001024"
     CDS             complement(7810..9825)
                     /locus_tag="KB942_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6851468.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10004..10309)
                     /locus_tag="KB942_001025"
     CDS             complement(10004..10309)
                     /locus_tag="KB942_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBB6851469.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10398..11429)
                     /locus_tag="KB942_001026"
     CDS             complement(10398..11429)
                     /locus_tag="KB942_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6851470.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11636..12511
                     /locus_tag="KB942_001027"
     CDS             11636..12511
                     /locus_tag="KB942_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6851471.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12663..13505)
                     /locus_tag="KB942_001028"
     CDS             complement(12663..13505)
                     /locus_tag="KB942_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6851472.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13597..15474)
                     /locus_tag="KB942_001029"
     CDS             complement(13597..15474)
                     /locus_tag="KB942_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6851473.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15471..16892)
                     /locus_tag="KB942_001030"
     CDS             complement(15471..16892)
                     /locus_tag="KB942_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6851474.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16895..20284)
                     /locus_tag="KB942_001031"
     CDS             complement(16895..20284)
                     /locus_tag="KB942_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6851475.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20541..21932
                     /locus_tag="KB942_001032"
     CDS             20541..21932
                     /locus_tag="KB942_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851476.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22005..22652)
                     /locus_tag="KB942_001033"
     CDS             complement(22005..22652)
                     /locus_tag="KB942_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6851477.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22660..23346)
                     /locus_tag="KB942_001034"
     CDS             complement(22660..23346)
                     /locus_tag="KB942_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851478.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23352..24425)
                     /locus_tag="KB942_001035"
     CDS             complement(23352..24425)
                     /locus_tag="KB942_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851479.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24647..25321
                     /locus_tag="KB942_001036"
     CDS             24647..25321
                     /locus_tag="KB942_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6851480.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25326..26666
                     /locus_tag="KB942_001037"
     CDS             25326..26666
                     /locus_tag="KB942_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6851481.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26815..27801)
                     /locus_tag="KB942_001038"
     CDS             complement(26815..27801)
                     /locus_tag="KB942_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBB6851482.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8147 a         5266 c         5057 g         9451 t
ORIGIN      
        1 aatcgtcatt tttccatcaa aatctgctta tgcaaaatta taaaaaaatt ttgcagtttt
       61 tcttactttt tttgtcgttt aattgaatga taaaaaccag tttctttatt cagttaatca
      121 aatatgaacg aattagatat gaatcattgt atttgccgtt gcggaaggat tttttaatta
      181 accacagata aacattgata acgattcgct tattgtaatc aacagattaa atttcatatg
      241 ttcatatgta cggatacatt ttcggaccga ttaattttaa ccattctatt tatttgctta
      301 cctattagat atcaataaaa aactgcttaa cagctatttc agctgttaag cagttttagg
      361 atttttagtt catattcatt gtttcttata atacagtgtt caatacttct cttaaagcac
      421 ttgttgattt gctgaacatt gctttttctt cttcgttgat tgataattca acaacttcac
      481 gaacaccatt ttcgtcaacg attgatggca cacctgtaaa gatatctttt tcgtcgtatt
      541 caccagttaa ataagcagat acaggcaata cagcttgttc gttattcaag atagctttta
      601 cgatacgtgc agtactcata ccaataccat agtaagttgc ttttttacga tcaatgatct
      661 cataagcagc attacggact ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat
      721 ggtctttttc tacaatttca aacactggtt taccaccgac agttgtatgt gaccatgcag
      781 caacttctga atcaccatgt tcacctaaga tatagccgtg gacgctgcgt gggtcaactt
      841 ttaatttcaa tgcgatttct ttacggaaac gagttgtatc caatgttgtt ccagtaccga
      901 taacacgtga tgttggcaga ccagattcgt tccaagcgat atatgtcaaa atatctactg
      961 ggtttgaagc aacaacgata ataccgtcga atcctgatcc catgatttct tttacgattt
     1021 ggcgcatgat tgacgcattt gttttaacta aatctaaacg agattgacca ggtttttggt
     1081 tgataccagc tgtgataaca acgatatcag catctttaca ttcttcatag ccacctgacc
     1141 atacagccac attttcatcg ccccaagcca taccatctaa aaggtccaat gcttcccctt
     1201 ctgctttttc ttgattcaca tcgatcaata ctaattcatt agagattcct tggttgatca
     1261 ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac gattactact ttacgacttg
     1321 tttttttcat gaataaacag ctccttttca taactatttg ttactttcat gaaaagtata
     1381 gtcttgttta ttcattttat caacatgtta attataatag ttgttattat aatttttgat
     1441 ggaaacgaat tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc
     1501 tgataataca accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat
     1561 ataataagac agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt
     1621 tcttcttaat gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat
     1681 ttgaataaag aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa
     1741 ctcgtttctt gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga
     1801 aaggaagcat caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa
     1861 tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact
     1921 ctaagcatct cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa
     1981 tgagtcaatt cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg
     2041 tatgtatcaa aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt
     2101 ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa
     2161 aaagaagaaa gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt
     2221 tcttctttta ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga
     2281 gtagcttctt tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta
     2341 ataatttgta aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc
     2401 tcgggaactt ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga
     2461 tcccctcggt ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata
     2521 ttcattcctt gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc
     2581 taattcagct agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag
     2641 ttcacgctgt actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg
     2701 ttgggacatg aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt
     2761 ttgataagcg cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt
     2821 tctctgctgt cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc
     2881 agtatgtcag ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct
     2941 ccctctttta tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga
     3001 caaaaacaaa ctgtgatcat cggattagtg attgcatcga tttgtggtat cctccccctc
     3061 ttctcacaaa gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga
     3121 ttgatcaatt ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc
     3181 tctatgattg gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt
     3241 gcaggacagc tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta
     3301 cctattcttg tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatcg
     3361 catggcacag ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt
     3421 atttttctct tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc
     3481 ctgacgactt attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa
     3541 ttggcaagta tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat
     3601 atgcttacac tttctttact actgatggga atcggctttg tattgattgc cactgtgata
     3661 aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt
     3721 ccttacttat tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact
     3781 tcaatcttat tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt
     3841 ctctcttttg cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg
     3901 ctgttgatta gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg
     3961 ataagagtag atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg
     4021 tgtaaaatga caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag
     4081 atccgccatg gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg
     4141 tactggtaca tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat
     4201 caaccttcca tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa
     4261 aaacatccat gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt
     4321 gtaggacata catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat
     4381 attggctgga cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat
     4441 gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc
     4501 actgaaacgg agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt
     4561 gtagaactaa tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc
     4621 gatgtagaag ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa
     4681 atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg
     4741 gttcaacttc aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc
     4801 gattcttctt tactgaatca gatctggtca gtatcagaag aagccttcaa gttatgtagt
     4861 gatttgtttt ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat
     4921 caatcattca tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata
     4981 ttagcattaa gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg
     5041 atcttgtggt tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta
     5101 cgcatcgtct accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa
     5161 ttagggttta tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat
     5221 aaagaaggaa cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc
     5281 acttgcggag atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc
     5341 tatcaagaaa aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa
     5401 cttggcgctt ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat
     5461 attttcgctg ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat
     5521 gtcttattga atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa
     5581 gatgctttat gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg
     5641 ggagggatgt tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca
     5701 ggaaatgaaa agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg
     5761 ggagcaagcc caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca
     5821 ggttatgaag aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg
     5881 ctacctatca aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact
     5941 tctgatagaa caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa
     6001 cagccacttg tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg
     6061 acatatcaga agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt
     6121 ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag
     6181 ataatacgac aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt
     6241 ctcctagttt cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag
     6301 aagcgatttt aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata
     6361 agctattgaa agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga
     6421 caccagaagc agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt
     6481 gcaagcctat catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa
     6541 ttaatacagc attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat
     6601 cgggtacgag ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc
     6661 gaaccccttg aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa
     6721 agattacagc gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca
     6781 aagcaagtgc gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca
     6841 aaaagctgaa acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa
     6901 actttccgat ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt
     6961 gattgtttcc agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata
     7021 gtgttccttc gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc
     7081 ttactaaaat gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa
     7141 tgaatttata tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga
     7201 tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag
     7261 gaactactcc ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat
     7321 tactgattaa accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc
     7381 cttggatcac ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag
     7441 aaacgaggat ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg
     7501 atagttcctc ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt
     7561 acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt
     7621 agcgtatctt tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt
     7681 ccaacatccg tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct
     7741 tgtttgatgt tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa
     7801 attttcatat cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc
     7861 gtgtaataaa tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc
     7921 ccgaagatgt gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc
     7981 tggattatca agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt
     8041 tacgccatct tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc
     8101 accatactgc tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc
     8161 tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga
     8221 taattggatc atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt
     8281 ttctaaaagt ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc
     8341 ttcttttaat tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga
     8401 aaatcctggt atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc
     8461 agtcatgatt ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac
     8521 agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt
     8581 agtctctgta aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga
     8641 agagctaatg ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc
     8701 gtccgctttt ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg
     8761 gatgctcttc cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat
     8821 ggaagcatat tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga
     8881 aatacgaaaa taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga
     8941 agcaaattct acggccatca ggaattccat gcgatggatg acttctgaag aacattccag
     9001 caaaaaatca agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga
     9061 gaaaaatata atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg
     9121 actaatgtca gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga
     9181 tgtttttcca attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt
     9241 tggtgcgaat cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc
     9301 gtaataatca atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga
     9361 ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag
     9421 cagcctaaaa ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt
     9481 tcttatcttt ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa
     9541 atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg
     9601 ctcttgttcg gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc
     9661 gattgctcgg aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt
     9721 taattgtttg atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact
     9781 gatttctctt ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc
     9841 ctacttatat tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag
     9901 ttggattttt tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca
     9961 ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt
    10021 tattttttga ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat
    10081 ctttttcaaa gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag
    10141 ataaagctgt ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt
    10201 tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac ttcttcttct gttttagggg
    10261 ccattgaacc tcttaatcgt ttgtgttttt caccgtcata atttgtcatg ttggttcctc
    10321 ctagtagttc tatacttttt catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc
    10381 cgattcttac aaatatgtca tatttttcct tctgattctt gaatgctttt gattccttct
    10441 tccaaaattt tcatttgtaa gattgtttct tcatctttga tgactttttc tgcgccatta
    10501 actaatgtct cgtaaatccc ttcgtagaca cggctgtaat caccaacctc ggaaattact
    10561 ttttcttcat gataactccc ttgttcatct atataggtca atacaccata atgttcgggc
    10621 aaatcgatac caaagtcttc atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt
    10681 cgatcttttg tttgtttcac gaacatccct ttttttccgt aaacgacgaa acttggacgt
    10741 tctttgatgc ggaaataact ggattttatc gaaattttca tatttccata gaaaaaatcc
    10801 agatcgaagt aatcattcat tcgtcctttc cctaaaagtt gacgtacatc atagctgata
    10861 tcatctggtt cgccgaaata agaaagaact tgatctactg tatgacaagc atgtccatat
    10921 aaatagctgt tcgcagtaga aaattcatgc gtattttctg gtacttccgg tctgaaataa
    10981 tcataatgca tttctacttc taaaatatcc cctaattttc cgctttcgat tactttttgg
    11041 actgtcaaga agtcagaatc gaaccgtctg ttttggtaac attggataaa tagacctttt
    11101 tcttttgcta atgcaaataa ctcttctgct tcctttgacg tttctgcaaa aggtttctct
    11161 actagtacat tcttaccatg ttcgagtgcc atttttccat attcataatg aaattgacta
    11221 ggtgtagtga tgacaactaa ttggatctca ggatcatccc aaagatcaga cagctgatca
    11281 gtatattcga tgtcttcgaa tgttttccac actggttcag atggggaaaa aatttttttg
    11341 atattgattt tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt
    11401 ccattgccaa gataagcgat tgtaagcatg agtggtgcct ccttttattg atgttttcag
    11461 tatatcctag aaaagcgcag aaagtattta taatagctat tctggtaaaa aagttcagaa
    11521 aaacacttgt tatcattcgg tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa
    11581 aagatagtag cggaaccaag tgtttatcag taagatagaa tttggaggtc agatcatgct
    11641 tattgaagaa aaactaacaa aacaagagtt gtttaccacg acagagaaac ggattgtcga
    11701 ttacatccgt aggaatatcg aagctgcagt ctacatgacg attgaagaat tagcaaaagc
    11761 tacgtatact tcccattcag ctatcattcg tttatgcaaa aaaatgggat tcagcggatt
    11821 taaagaattc cgtttcgagc tggggcggga agttcatcaa ttgattgctc agttcaatca
    11881 aacgattgat cctaattttc ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa
    11941 aatggcggat ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg aacatgaaga
    12001 tttagatcaa atcggtaaag tattgaacaa ggcacagcga atctttcttt ttgcaaaagg
    12061 tgattctcaa atcacggcaa ggaaattcca gaataaaatg gtcaagttga ataagttttt
    12121 gatcatggct gaagaataca gtgattcttc ttggaatgca gccaatttga ctagcgagga
    12181 ttgcgccatt tttattagct acagcggtcg catccatcat tatgaacgaa taatgaccta
    12241 tttgaagcat gtagaggccc ctaccttgct gattaccgga aatcagcatt cagaaatggc
    12301 caaacaggct gatatgtgtc ttgttatatc gcaggaggaa tatgattttg ctaaagtagc
    12361 gactttctcc tctcaaattg ccttcgatta cgtattaaat acgttattct cagtgatata
    12421 ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa
    12481 aggccttctt aaagaatatc ggaaaactta agaaaagatc ctcatttagt aataagaaag
    12541 atagtgtttg ataggctctc tttgatatca tcataaaaac aggctgaagt tttctttttc
    12601 aaacctcagc ctgtttgttt acggaacgat tcgaatagat atattataga aatgaggaat
    12661 agctaacttc tgtttcttat tttgaacgat tgacgaaaaa gatatataag aaggaggaaa
    12721 atcagtatac atgcagctaa caatccatac aggaaaaatc gtttgttctc ggatactttc
    12781 tgtacttctt tttgaacttc ttttgcatga taaggaaccc ggtgccctct gacaagtaag
    12841 cgatgactgt tgaccatata aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt
    12901 tcgattctca gatcttttgt atcagtcggt tctaccgtct tgatctgatc gacttgataa
    12961 gcgagtgtcc gtccattgat ttcgatatag aaagaatcac ctaaagtcag tttcggtaag
    13021 tctgtgaaca actttgcttg aggcagtcct cgatggctgg aaataacggc atgtgtattt
    13081 tctccccccg taggataaga cgttccttcc aataatgagg cacctttttc tagcagcaca
    13141 gtattcgttt catcaaaaat tggcaaatgg acattgatct cagggatcgt caaaataccg
    13201 atcgtatgtt tctcaaaata ggttctatcc tttttcgcag gttctttttt cttagtaaag
    13261 ggatctgcac ctggatttcc accttctttt gctaattgct ggtttttctt tgtcatgttt
    13321 tcttggattt tagccatgac ttcttcattt tctttgaccg cttgttgctg ataatgagag
    13381 atgatctgct gatccagata attattcagc gcgtcgctaa caaatggata agctagtatc
    13441 cctattcctg agagaagcaa gaaaagcatc agcagatcta gaagtttcct tttctttggt
    13501 ttcatttgtg ttctccttgt gatgactgtt tgataataaa agaaaaagga gcatttgcgt
    13561 aatccgctgc tcctttttcc aattcttgca aaaagactaa atctgactgt gcttgcgtct
    13621 agcaaagtac agtccagcaa tcagtagaag gactactcct gcaccgatat acacatagat
    13681 tcccttaccg cctgttgaag gaagtgtacc tttgtgttta tttggtattt tttcaggaga
    13741 aatcaactgt cctgctgtta cataagattg ttcatcgata gtaaatgcta cacggtttgt
    13801 taatggaaca tatttttctg gcgctttcgt ttcttccaga tagtacgtgc catattttag
    13861 acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa accgttgctg attctttcgc
    13921 cgatacccag ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact
    13981 atcttgatca cgaacgacga attctgctcc agcaagtgtt tggtctgatg taacgtcacc
    14041 atctacttta acaaatcgtt tgcccccagt aacgacatcg actgacggtg gtgtttgatc
    14101 atttgtatgc ccgttatcga catttgcttg gttagaaaat cctttggttg gatctgcttt
    14161 ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg ccaggagtta atgaaggaat
    14221 ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag aataatttac
    14281 tgggtcgatt tctttatttc catcatataa ggcataagca tacgttcctg aagaatcatt
    14341 atcaaatgtt aaagcagcgt catgagtatc gataagtttg aatgttgtgt acttgttttg
    14401 cgttccttct ttatcagcga tacctaatgg gatattgaca gaaatctcat attgaatttt
    14461 ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt
    14521 atcatttttg atggtctttt ctactggtgt ttggctattt gccaggatct caaaaggcgt
    14581 gattgtttgc ttttcaatca tttccgcatt atcaggagct tttacttctt ctaaattata
    14641 ttttccaact tctaaatgat taacgatcaa ctggcctttt tcaatagatg cctcggcaaa
    14701 gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt tggtttgatc
    14761 agttgtccaa gtgtacaatc catctgtgac actttggatg tattttttga cgcttggtgt
    14821 tccttcttct ttagaaataa taaattctgc tccatttagt gcttcgtttt cggcagtacc
    14881 gatttttgta actttcaacg ttccatcatt accgactgta tttttagggt agagatggat
    14941 agtatctagt tcttctgtcc cgtatttata agagccatct gcttgtttga tcatctcata
    15001 tacagggaaa gctaaaacca tgtttgcggc agctgacact ccgtcttttg gttcttcttt
    15061 gatcgtgtag actgcatctt tcccattttg ttttttaggt aaagataaag tgacatttcc
    15121 atcagcatct gtcgttcctg aagcaactgg tgtaccagga gtcaaagact ggactgcttg
    15181 ttttgctgca tccacggacg ctcctttatc tcgttgcgca taaaattctt gagtgacgtt
    15241 ataaactgaa aatgaaatat cggctaatcc ttggtattga tcgaattcgc tcatttcttt
    15301 cccgctgttt tggattaaag gatcgggtaa atcagtcatt ttctttttat gaaggatgac
    15361 ttgagctgct gtctcctctg caaacacttt ttttgcgccg aagctgtttg tgagtaatgg
    15421 taaaataagg aaaaggactc ctaacataac gtttattttt ttatgatttt tcattttttc
    15481 actcctgttc tttttttgat aaacaaccag attgcaaagc ttgcgaatac cataccaaca
    15541 acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa gaaatggacg gctattttct
    15601 tttttattga ctactttcaa tatactgtcc gcctctttat cctcagaaag aaaagttgaa
    15661 atggtaaacg gaatttctgt ttcacttaaa atataaccag aaggtgcttc gatctctttt
    15721 aatcgataac ttcctgtttt caacccggaa attgaaaaag cgccattttt atcggaaatc
    15781 aggtgaagcg catctgattc gttctttgtc cacttgtatc cgttggctgt ttgctggagg
    15841 taatttcctg ctttgttttt aataaggaat actgcgccag ctaatcgttt atctggttta
    15901 tttgtctcga ttttgacaaa ttgcttactc cctgtatata cagagacttt ctttgtactg
    15961 acaccaaaac caaaatccag tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta
    16021 ctgcttagct gcatctgata agaaatggtc agtttttttc cagcatattt ttccaacgaa
    16081 gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc
    16141 agcttttctc cattcgatga aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc
    16201 gcactatctg acaattcgaa cttttgatag tcaaggatat ttacaggaat tgtcgtatgc
    16261 aaggaataag tgattttttc accaatcgta aaatcatttc taggctcttc taataccttc
    16321 tcaaaaggtg gatcagggta gtcgttttct tcatttttag gatacaaatg gatagttgaa
    16381 agtttttgat tgttttgtcc atgtacagga agaacaacta ccatattctc tgccttttct
    16441 ttgacgactt ctggtacttt ggattcaatg aaaagatacg ctttatctct tttttcttga
    16501 tctttagcgg ccagagaaaa agaagcgata ccatcttcgt tgttaagagt tgtagttgtt
    16561 gcttctgcgg taaacatacc ggaagacgca ccgttttttg cgatctctgc ttgtgcttct
    16621 tctaccgttt tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg
    16681 aatgtgacac catttaatcc tcgatacgtt tgtaataaag ctttttcttg tccgtcattt
    16741 ggatgatttt tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc
    16801 atttctgttt ctgtttctgt tgctacctga gtaaaagctg gtttaaatag taacaagaag
    16861 agacacatac taagccaacc aagttttttc atatttagct gccccctctt tcttttttca
    16921 aaaaaagata ccaaatagaa aacgcacaac caatcatccc tactagatag attcctaaac
    16981 ggccaattcc tcccgtttca ggtaatggta cttttgcctg attcgtgatg tctaaagaaa
    17041 tctggttgtt tttggctcct ggtgacagaa cagattcatg atcttgtcca tccacttgaa
    17101 ttgacccatc ttcgtgtata actatagtaa ctggctcttt tagaccttgg tatccttctg
    17161 gtgtaaaagt ttcggtcagc gtataagttc caggttttaa attttcgaat agaaaggtat
    17221 ctgtttcttg tccatctttt ggcgattcta agtccatttc tggtccctgc agtctgaatt
    17281 ttgctccttt taatgtctgt ccgttgtctt cttttttgtg taccgttaga tcaaacgctt
    17341 ttagtcgatt ttgatgagtg agcgtccata cattatttgt catctcggta ttttttcccg
    17401 tgtaaatgat ttttccatat ttatcgattt ggaattcata atttcccggc gaagtatcat
    17461 atccggcagg accagctgtt tcaacgactc gatatttacc aggtgtgagt gaatcaaaac
    17521 tggcaagacc tttttcattc gttgtttggc tgtcaattgg ctgataagta ccatttgctt
    17581 cttttttctg tagggaaaat gctgctcctt taagatttat ctctttgtcc gtcccttgca
    17641 tcgcatactt acgtactgct acaggaactt caacaaaagg attttcaata gtcaactgga
    17701 tcgtatcatc ggaagtagtg actgtttttc catcaatggt taccgtacca tcagaagcga
    17761 tcttgatttc ccaagtagtc tttttgctta atccatgccc ttctggagct tttgtttctg
    17821 tcagcgtata ggtcatttct ttttgcaatt tgacattttc tggaagagaa taggttccgt
    17881 cgccatgatc tgttagtaaa gtatcaatag aatctcctgt taatttgaaa acagcgccaa
    17941 taagatcctt ttcacctgta gaggaatttt tcgttatttt caagtttaac ggtgtgaatt
    18001 gtttagtatt tttccatgtc attgcatcta tttgttgaga atcgtatcct ggtacaggta
    18061 attctttgat tgtttggtaa ctgaatgctt gaccttgatt attgtattga ggtagtgata
    18121 atatctcttg ataactttct ccgctatttg cagataattt gtcaatgtct gcacgttccc
    18181 acgtatttgt tgtatcttta gctggtttaa tgattcgaat atatccgttt ttccaagctg
    18241 cagcatttgt cgtatgttcc cgttgaatct caaaagtaac ttgatctgga cgatcagcta
    18301 gattgttgtc aaattcttcc cataactttt tgatgtgaag actgactccg ggagctttag
    18361 cagatggtat accgaattca gctaattcat tggtatcaat gtttggctgg aaagttgtcc
    18421 tgccgttcat ttgataccag aaatttggat ggaagtcctc attttctgtt tggattctca
    18481 cttggtaatg gatttggatt tcttggtctt ttcctaaata gatctgattg ctcttgatgg
    18541 tatttccttc tatggaaata gatggagtga ccgttgtagg acttgtcccc acactcttga
    18601 ctgaaagtgt accaggctga taaatgaatg gttctgcgat tggatcattt atttgtccat
    18661 tgcttacagt tgctgagatc tgtacagctt ttttggctag gtattcagag atatctgttg
    18721 catgatcagc tgattcatag taaagatcgc ctttttcatc tgatgaaacc atttgtcgca
    18781 tacgagactc tacttctgct tttgagagac cagctgccgg atcgctttga agttggatgc
    18841 caagaccatg tatttcgatt cctcgttctt tgagtgccat cgcttctccg atggttgcga
    18901 taaacgtact gtcgattctt ctggatagat tgttttggtc aggtgcatca tagattcttg
    18961 agattagaga agtatttccc ggccgatctt gcgtattaga aaactgagtt ccgtaataat
    19021 tgctgcttga ttgtgcgtgt acccgctgta ctttatagga aaatgttggt acaccatccg
    19081 tcagcaaaac gatcactttt ttatgtccat ttggaacgga tagcatgctt cctgcatctc
    19141 ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg gattttactt
    19201 gatttttcac tgaatcaaat gaacccatct gtactgcacc gttactgtag ctatatcctt
    19261 cgcttgagta gccgacatat cccatattga ttttgtctgt gataccgcta tctgctaaag
    19321 tatcgacaaa acgatcgaca ccaatcttta cttcaccgat ccgattattg tcgttcatac
    19381 ttcctgacca gtctacgacc aatacgagat caagaggcgt gatatctttt tgtacatttc
    19441 cacgagcatt caaataaaca tcaaacaatc cgggtgtact tgtttccttt gcatactttc
    19501 gtatcgcata gtctgcttga ttaccggttc ctccatattc gatatacgaa tgggtccgat
    19561 cattaggatc tccattccaa gaatttatac catcccattg atttgttccg tttttattgc
    19621 cttgatgatc aagaacattt gtatttcctg taggttgcca actggcttta ggataagtgc
    19681 cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt agatggcaca ctcacagctg
    19741 gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct tttgatgatt
    19801 cttcttctat ttcgtctggc tttggtactg acacatctgt atctttctta ttttctgtct
    19861 catcaataat gtcatattct ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa
    19921 gtgctggatc gattggaaaa ctcagatcga tagtttgttt agtgtttgat gagatttcca
    19981 acgtaatttt cccgctattt acctgataag catagccatc tccttctttt ctttccacat
    20041 cacttaagta attttcgact agaccaattt ctttttctat cttttcagca ctggtgttat
    20101 tcgcatttag cttgatgttc agtgcttttt ttccagactg atccgaaatg acttcagctg
    20161 aaattgtttc taaaactgtt ggatcatctg tagtgtctgc tattgcagat aatggcacaa
    20221 agtttgataa actcaatatt atcagcatga aaataacttt cagtttcttc cctgtggtcg
    20281 tcatttttcc cctccttttt ctttttttct gttttgacgt gaatagtgaa aattattaaa
    20341 ataaacaggc gttacataat tttatttatt ttaatatgat aattattata cgaagggcgc
    20401 taaagggaat cacaccaatt ttcataccaa ttggtttcat ttatgaagtt gattccttcg
    20461 attaatgagc gtttcattga aagaaaaggg ttgagggaga aaaacatatg atcaggttgt
    20521 tggaaggaaa ctatagaaag atggaaaaaa tcattcgaat tctgtccatt tctgacaagt
    20581 ggatgactaa aaaagaactt gccaaacgaa ttggtagttc agaatctaca tttgtaaggt
    20641 atatcgaaga gatcaaaatc cgctggggaa cggatgtaac gattcaatct tcacaaaaat
    20701 tggggtatcg tcttgaacat ttgaatgtac cagcttattt aaatgttctg atggaaatgt
    20761 cgcaaagttc tacaaacagt cgtttattaa atgagattat tgtcaatccg ggtaaaacga
    20821 ttgagtatta ttgtgaaaaa atttccatca gccggtcttc ttttacacgt aagttgaaac
    20881 aatgcaatca aattttagaa agctatttat taaaagtgat cgttgaccaa ggttttcagt
    20941 taactagcct taaaagtgag ctccagttgc gtatctttgt tacctttttc tttttaagtt
    21001 actacggaag acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg
    21061 tcaagaataa ttgtcagatg aatttgtttc aggaagaaag cccatatgag caatctttct
    21121 ttattatgta ttttgttgtg catctcatga gagaatccca aggaactcca tttgaatgga
    21181 aagctggaga ctcttatgta cagttgataa atatcgataa aaaagacttc ttaatcttga
    21241 aaaatctctt aggaaaattg aagtacagca gctatataaa agtagtgaac tattttgtca
    21301 atacgatttt ttcagaattt tcggagaaag aaaaaaatga aatcagtgag cagatacgaa
    21361 aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg
    21421 ttgttcaatt attaacgcaa atttattttt tctcccgtat tttcccatac gatacgactc
    21481 atttgacgat ccgtgcaact tactttgcac agcagatgca acaccgccag atacgtctat
    21541 atgctggatt tcgtgcagaa cttcaatttt attccactct actgaatcag aatctgattt
    21601 attatttatc tagctatatc ttttggatgc ttatcggtaa tcctgaaatt caccattata
    21661 cttctgataa aaaaatactg attatcagcg acttaagtct tcgacatagt caatatattg
    21721 aagaatatat cagtgatatt cttgctgtgc ataaaattca ctctgagaca actgctatta
    21781 ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt acgaatcaac
    21841 ccattttgaa tgcccatgtt cctcatatat tgattgatga tagtgtgtca ttcgcaaatg
    21901 aagaagaact catccgctta ttcgagttat gaataatcaa aaaacagcta tgtataaacc
    21961 accctgataa aaatttcagg acaaatacac atagctgttt ctttttattg tattccttca
    22021 gcaatccaac gagatttata gcgaaagtct tttaacaact cttcttttgt ccaattattt
    22081 gcaatgagat ccgtcaaagt gttcattacg agggtcatgg aaaagtatag gaaactaact
    22141 gcttcttccg acgattgaat ggagaaatga ttgattctta ataattccag ccatttttcg
    22201 ttcattggag aatcagccga tagttctcta cgtttggttg tctgctgatc atttcgctgg
    22261 gttaacaagt ataacctttg ccaatagata ctttcgtgat ctaagtcgca gcaccactct
    22321 aaatattttt cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata
    22381 tcttgatgga ctatgtgaga actgcttttg atcgtataca aataagcatc ttccaaatct
    22441 tgaaaatatt tataaaaagc accgcgcgac atccccatct tgtcaacgat ttctgagact
    22501 ttgacttggc tgatgtgctg tgagcaaaat atctccagta ataggtcatc gattctctgt
    22561 ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca tccacatctc tcctttatct
    22621 ctgattatac gtggatagcc cttcattgtc atgaaggatt cattccttct ttttctcttc
    22681 ttctgtcaag tacccatctt ccattcgata aacgcgatca ctccattgaa tcatacggac
    22741 atcatgagta accataatag tcgctttttg tttctcatga gcttctttaa ctagtaattt
    22801 gacgacttca tatgcatgat ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa
    22861 gcttggctca ttatataaag cgcgagcaat tgccactctc tgtctttctc caccagaaag
    22921 gtctttagga taactatttt taagtgactg gatatctaaa gattgcagca aggaatcgat
    22981 ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact agttcaaatt gttcctttac
    23041 cgttaaaaaa ggaatcaaat tagagctttg gagaataaat cctatttctt taaagcgtaa
    23101 atctgctcgt tttttttctg gaagctcagt tagtgagtga ccgttgatca ctatttctcc
    23161 ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga
    23221 aggaccaatg atgctgacga attctccttc ctttacataa aaatcaattg ctttcaaagc
    23281 tgagaccttt gtatgaccag aaccaaatgt tttggtaact tttttcatcg ttaatatttt
    23341 tttcattttt attaacctcc tattgctgta attgggtcta ctttggttat cgtacgtatc
    23401 gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc cactatatag caaccattgt
    23461 gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa ataagcaaac
    23521 aaaagagcaa gcagaacgcc taccacccca acaataaagg actgtgctac taaagattta
    23581 gcgatgaaga aatttttgat cccttgtgct ttcattactc caaaaatagc agtcttctga
    23641 agtgtcatca cgtacatgaa aataccgacc acggcagcaa ccactaagaa caaaaagtat
    23701 atcatcgcat ttaaggtcat attttgggca gaatagccag gaatactttc aatgaatgtc
    23761 ccaatagtta ataactgtag acccttcgat gctttttctt tcgaaatttt agctgaatcc
    23821 tttattacga tcccattgat cggctgttct tcttcagaag caaacggctg ttgtccatac
    23881 ttcaaagcag tccacgtatc tagggacgca tagatgacgg gagaaactgt gtaataagtt
    23941 tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct
    24001 ccaattttat agcctgaatc agccagattt tgagagatga tgatttcatt ctttgtttta
    24061 aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact
    24121 gtaacatttt ctttgttttt tcctttgatt gctccgctgt acaatccgat tggtgctttc
    24181 tctcctcctt caatgtattt cagatctcct cttgttaatt gtgaagctgc aaatacttga
    24241 tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat catcgattgc ttttttaaac
    24301 tcttctgaaa gtccattcgc tagtcctgac aacatgaata cgacatacgc aatcaagaac
    24361 ataataccga cgatcaagcc atatcgtaat ttagaatagc gaatttcttt gatagctaga
    24421 aacatttgtt tttcctccct atgatagatt gatagtgaca aaatgtcact tgttgaatag
    24481 tgtaactcta aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa taaattgtct
    24541 aagacatctt tcgataaaac cttaacatga gtcataaaaa atttatttaa gccttattta
    24601 tgctttccat gataaactaa agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt
    24661 agtagtagaa gacgataaag tattatccaa taatataaaa gaaatactcg ctgcaatcgg
    24721 cgagataaca caagtttatg atggtagtga aggactgttc gagttggaaa gtggagttta
    24781 tgatcttgca atcttggatt tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa
    24841 aatacggaaa gaaaaaatac agactccagt attgattttg actgcaaaag atagtttgga
    24901 agataagatt gaaggatttg aacgaggtgc agatgattat ctaacaaaac ctttctatcg
    24961 agaagaactg atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc tctttgatga
    25021 aaatgttctg aagtataagg atatcagttg tgatttaaaa agcaatgaag tcttgtataa
    25081 agaacaagtt ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa
    25141 taagggaatc attttgacaa aagagcagat atttgaccgg atctggggat ttgattctga
    25201 tacgacgatt acagttattg aagtttatat gagcaactta cgaaaacatc tgcgaagtac
    25261 tggattggat aaagaaatca aaaccttacg taatgtcggt tatatactgc aaagggtgtg
    25321 atgcaatgaa tggagatatt acacgaaagc aacgtagtcg cttttttgcg acaaatgtag
    25381 cagcatttgc ttttatcttt tttcttttgg gattcattac tttacagata ttaaaccaat
    25441 ctgcatacag agaaacagat ctgtcattag agaaaataaa ttttgattct cgaacagtac
    25501 aattagagat acaaagatat cagcaaggcg atcccttttt aaacccgact caaacggaac
    25561 aaaacagaac aaacatggaa cagccattaa tggaaccatc aaatgaccgt tttaacactc
    25621 aagtgatctt gtggtctaaa aatggtgaga tattgaataa agcagctatt ggtgggcgtt
    25681 tcaaccaaat caaagaatta aaaatggata cgaaaaatct gaataccatc caaaatattg
    25741 aattaaatga agacagtggc tcatctgccc tctctttccg ttcgattacg aaaaaagcgg
    25801 agacgaatca agatggcgtt gcctacatcc aaattttaga aaatacaaat caaatcaaga
    25861 attcattagc gacttttcgc acgattatga ttttgtgtat gattgttttt tggattctct
    25921 ctattggtat cagttattac ttgtctagtc taagcatgcg ccctattatt ctttcttgga
    25981 gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta
    26041 ttcaaaatag tttggaacat ctttttacca agcctaatca tactattatt gaagaatccg
    26101 aaagcatcgc tcaagcattg tcagagacga ggcgcctcac tggattgact tcggatttgc
    26161 tgacaatcgc tcggaatgat tctgatcagc agctgctttc aaagcaaatg atcaatacac
    26221 aagaatatat cgaacaactt gttaaaccat ttcaagagat ggccatcata gatggaaaag
    26281 agtttattct ccataacgat gcaacattag aagtaccaat tgatgaaaaa aaaatccatc
    26341 aagttttggt tattttgctg gataatgcat taaaatatac gaatccagaa gataagatca
    26401 ttttggattc atctgttaac cataaagaat ggctgattca cgtaaagaat actggccctt
    26461 ctatcagtga agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc
    26521 gggcgaaaga gactggcgga tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac
    26581 agcatcatgg acagatttct gtatccaatt atcttccaca aggagtcgat tttaaagtcc
    26641 aattgccgtt gaagcctaaa aaataatgct tatccctaaa aaagggctgt gacaaaaatc
    26701 ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca gaagtagctt cttcggaaat
    26761 aagccgaaat ttctttaagc tgaccacttc tgtcacaacc tctttagctt acgcttacga
    26821 ttttttattg cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca attcttctcg
    26881 gctaacatct tctgtcactt tttcaacatc ttcatgttct tctttatgaa tcccaacttc
    26941 ttcacgaata actgtatgtt tgcttacatc tacttgctct tctttgattg ggatagtaat
    27001 cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg
    27061 ttcgatgacg atttcttcac gtttgaccgg tacttctacc gtttcagtat cgttgaccac
    27121 atgtttgtgg atgtcgactt ctccagttgt cacatcatgt gtatttacat ctaatttttc
    27181 ttcttttagt tgaattgtat catcatctag atttggtttg cgatcgatgt cttcagtctt
    27241 gcttgtaccc attgtatcag tctcgccagg tacgtccatc ggatcagttc ctggtgtcat
    27301 attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt
    27361 tccagtgcca gtttctaatc cagtccccat tgtgcttgct gggctaacag tatcttgtgt
    27421 atccattgta tcttctgttg tttctggctg acgatagcct tttactgtga tgacaaattt
    27481 tccatcagaa atatctcctc tatattgata taaaggatca ttttctggtg tagccgtctc
    27541 agaatcatag gtatcaaaag aaaaagtatc ttttattttt tcccataaag aacggtcttc
    27601 ttcaccatcc atccgatttt catctgtttc aacatctata ccagacaatc cgtcaaatcc
    27661 tagtctagat gctgcttcat cagtagtata aataacgata tcatctcgtt catacccttc
    27721 gttacgcaac cgttcaatta ctgataatgc ttcttccctc gtactatagc ttccgatgac
    27781 cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa tagatttatg attggtatat
    27841 tgttacttat aaatttattc tataggagac gattgttttc tacaaatatt tagattggaa
    27901 tttgattaaa acaatttaat t
//
LOCUS       DADVXN010000028        27705 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.029, whole genome shotgun sequence.
ACCESSION   DADVXN010000028 DADVXN010000000
VERSION     DADVXN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.029"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            20..748
                     /locus_tag="KB942_001039"
     CDS             20..748
                     /locus_tag="KB942_001039"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBB6851483.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            748..1002
                     /gene="purS"
                     /locus_tag="KB942_001040"
     CDS             748..1002
                     /gene="purS"
                     /locus_tag="KB942_001040"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBB6851484.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1004..1675
                     /gene="purQ"
                     /locus_tag="KB942_001041"
     CDS             1004..1675
                     /gene="purQ"
                     /locus_tag="KB942_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBB6851485.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1676..3898
                     /gene="purL"
                     /locus_tag="KB942_001042"
     CDS             1676..3898
                     /gene="purL"
                     /locus_tag="KB942_001042"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBB6851486.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3883..5322
                     /locus_tag="KB942_001043"
     CDS             3883..5322
                     /locus_tag="KB942_001043"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBB6851487.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5345..6397
                     /locus_tag="KB942_001044"
     CDS             5345..6397
                     /locus_tag="KB942_001044"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBB6851488.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6394..6972
                     /gene="purN"
                     /locus_tag="KB942_001045"
     CDS             6394..6972
                     /gene="purN"
                     /locus_tag="KB942_001045"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBB6851489.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6969..8504
                     /gene="purH"
                     /locus_tag="KB942_001046"
     CDS             6969..8504
                     /gene="purH"
                     /locus_tag="KB942_001046"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBB6851490.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8520..9794
                     /gene="purD"
                     /locus_tag="KB942_001047"
     CDS             8520..9794
                     /gene="purD"
                     /locus_tag="KB942_001047"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBB6851491.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9916..10830
                     /locus_tag="KB942_001048"
     CDS             9916..10830
                     /locus_tag="KB942_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6851492.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10963..12528)
                     /locus_tag="KB942_001049"
     CDS             complement(10963..12528)
                     /locus_tag="KB942_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6851493.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12521..13972)
                     /locus_tag="KB942_001050"
     CDS             complement(12521..13972)
                     /locus_tag="KB942_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6851494.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13986..15002)
                     /locus_tag="KB942_001051"
     CDS             complement(13986..15002)
                     /locus_tag="KB942_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6851495.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15222..16310
                     /locus_tag="KB942_001052"
     CDS             15222..16310
                     /locus_tag="KB942_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBB6851496.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16364..17890
                     /locus_tag="KB942_001053"
     CDS             16364..17890
                     /locus_tag="KB942_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB6851497.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17892..19097
                     /locus_tag="KB942_001054"
     CDS             17892..19097
                     /locus_tag="KB942_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851498.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19354..20331
                     /gene="guaC"
                     /locus_tag="KB942_001055"
     CDS             19354..20331
                     /gene="guaC"
                     /locus_tag="KB942_001055"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBB6851499.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20595..22019
                     /locus_tag="KB942_001056"
     CDS             20595..22019
                     /locus_tag="KB942_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6851500.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22480..23589
                     /gene="pdhA"
                     /locus_tag="KB942_001057"
     CDS             22480..23589
                     /gene="pdhA"
                     /locus_tag="KB942_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBB6851501.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23592..24569
                     /locus_tag="KB942_001058"
     CDS             23592..24569
                     /locus_tag="KB942_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBB6851502.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24594..26237
                     /locus_tag="KB942_001059"
     CDS             24594..26237
                     /locus_tag="KB942_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBB6851503.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26245..27651
                     /gene="lpdA"
                     /locus_tag="KB942_001060"
     CDS             26245..27651
                     /gene="lpdA"
                     /locus_tag="KB942_001060"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBB6851504.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         8995 a         4775 c         6311 g         7624 t
ORIGIN      
        1 ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa
       61 aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc
      121 gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac
      181 aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt
      241 gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg
      301 aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat
      361 caaaccaatc attgaattct actataagga tgaccagttg gatgatccat ttatcaatga
      421 agagcatatc tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca
      481 agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt
      541 agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac
      601 accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta
      661 ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga
      721 actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa
      781 gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat
      841 gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa
      901 gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg
      961 gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat
     1021 cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg
     1081 cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt
     1141 actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc
     1201 tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg
     1261 caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga
     1321 gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac
     1381 ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta
     1441 tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta
     1501 taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa
     1561 tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga
     1621 tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa
     1681 aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg
     1741 attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta
     1801 tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa
     1861 accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg
     1921 cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa
     1981 tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag
     2041 agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga
     2101 attagataat ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata
     2161 cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga
     2221 aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa
     2281 agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga
     2341 cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg
     2401 ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga
     2461 attaatcttg catcattcgg attctctaat aggtatacag gatatgggag cagcaggact
     2521 tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga
     2581 tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca
     2641 agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca
     2701 gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct
     2761 atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc
     2821 agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc
     2881 ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac
     2941 gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt
     3001 tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc
     3061 tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat
     3121 agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac
     3181 cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc
     3241 agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt
     3301 ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt
     3361 cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct
     3421 aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca
     3481 gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca
     3541 agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga
     3601 aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt
     3661 acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac
     3721 agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc
     3781 aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat
     3841 agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag
     3901 agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg
     3961 gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc
     4021 aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa
     4081 gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca
     4141 acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag
     4201 tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta
     4261 gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc
     4321 cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa
     4381 ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat
     4441 ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa
     4501 acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata
     4561 gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct
     4621 atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat
     4681 gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca
     4741 gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct
     4801 agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc
     4861 cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga
     4921 gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc
     4981 agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct
     5041 tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc
     5101 attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt
     5161 tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca
     5221 ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa
     5281 aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga
     5341 gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata
     5401 tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg
     5461 cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt
     5521 ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa
     5581 acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg
     5641 cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt
     5701 ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg
     5761 cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt
     5821 tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga
     5881 tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa
     5941 aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac
     6001 acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat
     6061 caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat
     6121 cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct
     6181 tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat
     6241 cttcaatatg ggcatcggta tggtcctggc agtccaagaa tcgaaagtaa aagaaatagt
     6301 acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga
     6361 aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga
     6421 aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg
     6481 attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg
     6541 agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa
     6601 gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg
     6661 cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg
     6721 tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta
     6781 aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc
     6841 cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc
     6901 catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc
     6961 tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg
     7021 cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt
     7081 tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga
     7141 tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg
     7201 atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt
     7261 gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag
     7321 ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg
     7381 aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc
     7441 agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata
     7501 ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac
     7561 cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc
     7621 atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa
     7681 aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac
     7741 ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg
     7801 gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg
     7861 tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac
     7921 agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg
     7981 aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa
     8041 atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc
     8101 tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag
     8161 aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg
     8221 tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt
     8281 cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg
     8341 atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa
     8401 tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg
     8461 atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa
     8521 tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag
     8581 ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg
     8641 gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga
     8701 aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg
     8761 atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg
     8821 aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat
     8881 ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa
     8941 tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga
     9001 aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca
     9061 aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc
     9121 gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg
     9181 ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa
     9241 agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac
     9301 taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa
     9361 aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt
     9421 tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa
     9481 agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag
     9541 aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg
     9601 ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag
     9661 aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata
     9721 aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac
     9781 tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag
     9841 cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa
     9901 taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca
     9961 gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca
    10021 gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt
    10081 gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt
    10141 caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt
    10201 gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt
    10261 catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa
    10321 cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg
    10381 ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat
    10441 actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat
    10501 gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca
    10561 taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac
    10621 caaacattag aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct
    10681 tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga
    10741 atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg
    10801 taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat
    10861 aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt
    10921 ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt
    10981 aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata
    11041 actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc
    11101 tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa
    11161 tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg
    11221 ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa
    11281 tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa
    11341 gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa
    11401 gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca
    11461 gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt
    11521 gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga
    11581 caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat
    11641 caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg
    11701 cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac
    11761 agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata
    11821 actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga
    11881 ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact
    11941 gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat
    12001 ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt
    12061 aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc
    12121 agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc
    12181 ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat
    12241 tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg
    12301 aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc
    12361 agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc
    12421 agcttcgccg acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa
    12481 gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc
    12541 caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc
    12601 tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt
    12661 tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta
    12721 tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca
    12781 tctcatcaat tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc
    12841 catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa
    12901 aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga
    12961 tgactagata atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt
    13021 tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa
    13081 agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag
    13141 taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt
    13201 tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt
    13261 gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt
    13321 ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat
    13381 tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa
    13441 tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa
    13501 aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt
    13561 cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt
    13621 ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg
    13681 tattattttt gatctctcga atatcattcc taagttcttc taggatattt tcctcattga
    13741 actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat
    13801 caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca
    13861 aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca
    13921 tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc
    13981 tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg
    14041 gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta
    14101 acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt
    14161 cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct
    14221 tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca
    14281 ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc
    14341 tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga
    14401 ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa
    14461 aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt
    14521 ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt
    14581 ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga
    14641 atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag
    14701 tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac
    14761 gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg
    14821 taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc
    14881 tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt
    14941 gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc
    15001 actttaaatc actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca
    15061 aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa
    15121 agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta
    15181 ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa
    15241 agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt
    15301 caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag
    15361 aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa
    15421 cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga
    15481 ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg
    15541 tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga
    15601 attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag
    15661 cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat
    15721 tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg
    15781 ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca
    15841 gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt
    15901 taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa
    15961 gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac
    16021 ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac
    16081 aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca
    16141 aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg
    16201 tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag
    16261 cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa
    16321 acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt
    16381 aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct
    16441 gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac
    16501 attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta
    16561 tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat
    16621 cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg
    16681 aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga
    16741 actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt
    16801 agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat
    16861 tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat
    16921 ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat
    16981 cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga
    17041 aaatgaaatg atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga
    17101 tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac
    17161 ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa
    17221 aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat
    17281 gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc
    17341 agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga
    17401 ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag
    17461 cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc
    17521 agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag
    17581 tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga
    17641 tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta
    17701 cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact
    17761 tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg
    17821 agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga
    17881 ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt
    17941 tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac
    18001 tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta
    18061 accttgttct gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc
    18121 tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg
    18181 gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag
    18241 taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt
    18301 ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag
    18361 gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag
    18421 acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga
    18481 tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag
    18541 tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa
    18601 taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga
    18661 tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga
    18721 cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct
    18781 gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat
    18841 tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat
    18901 attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg
    18961 gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc
    19021 agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga
    19081 aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt
    19141 gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa
    19201 taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta
    19261 attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt
    19321 tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt
    19381 caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca
    19441 ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat
    19501 gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat
    19561 gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc
    19621 agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta
    19681 aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg
    19741 attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca
    19801 ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt
    19861 ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg
    19921 gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc
    19981 agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt
    20041 tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg
    20101 tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa
    20161 ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa
    20221 caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa
    20281 gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat
    20341 aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa
    20401 aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa
    20461 catcattatt cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc
    20521 aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata
    20581 gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa
    20641 ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg
    20701 aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat
    20761 tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta
    20821 atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc
    20881 ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa
    20941 aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg
    21001 agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag
    21061 gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat
    21121 tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac
    21181 aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat
    21241 tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga
    21301 tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca
    21361 acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag
    21421 tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt
    21481 catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat
    21541 acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac
    21601 ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata
    21661 tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag
    21721 atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat
    21781 cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact tgggatggga
    21841 aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa
    21901 gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa
    21961 accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat
    22021 ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct
    22081 tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc
    22141 cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt
    22201 taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat
    22261 aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa
    22321 tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat
    22381 ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg
    22441 aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg
    22501 atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt
    22561 gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc
    22621 gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg
    22681 aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg
    22741 gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct
    22801 caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct
    22861 gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca
    22921 caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc
    22981 gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat
    23041 ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc
    23101 tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct
    23161 gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa
    23221 atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat
    23281 cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca
    23341 gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa
    23401 ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa
    23461 gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac
    23521 atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag
    23581 tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg
    23641 aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag
    23701 ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc
    23761 ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc
    23821 cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc
    23881 aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt
    23941 cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga
    24001 tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac
    24061 tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat
    24121 atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag
    24181 cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag
    24241 caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc
    24301 gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg
    24361 tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga
    24421 tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata
    24481 cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag
    24541 acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt
    24601 atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt
    24661 tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca
    24721 aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag
    24781 aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag
    24841 acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg
    24901 aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt
    24961 taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag
    25021 gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag
    25081 aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag
    25141 caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa
    25201 ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg
    25261 tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg
    25321 cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa
    25381 caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag
    25441 aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg
    25501 caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc
    25561 caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga
    25621 cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag
    25681 ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag
    25741 cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg
    25801 tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac
    25861 caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga
    25921 aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta
    25981 cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag
    26041 aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg
    26101 aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg
    26161 gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat
    26221 taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca
    26281 gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga
    26341 caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt
    26401 attccttcaa aagcactgat agctgcagga caccattatc aagagtcact agattctgaa
    26461 atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa
    26521 gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag
    26581 gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat
    26641 ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca
    26701 atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg cggattagca
    26761 ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg
    26821 ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt
    26881 ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc
    26941 gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca
    27001 gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact
    27061 gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga
    27121 gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc
    27181 atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc
    27241 gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta
    27301 gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct
    27361 ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt
    27421 ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc
    27481 gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa
    27541 tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa
    27601 atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa
    27661 tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt tttta
//
LOCUS       DADVXN010000029        26764 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.030, whole genome shotgun sequence.
ACCESSION   DADVXN010000029 DADVXN010000000
VERSION     DADVXN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.030"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1408)
                     /locus_tag="KB942_001061"
     CDS             complement(<1..1408)
                     /locus_tag="KB942_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6851505.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDI"
     gene            complement(1419..2087)
                     /locus_tag="KB942_001062"
     CDS             complement(1419..2087)
                     /locus_tag="KB942_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6851506.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2217..2801)
                     /locus_tag="KB942_001063"
     CDS             complement(2217..2801)
                     /locus_tag="KB942_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851507.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3132..3365)
                     /locus_tag="KB942_001064"
     CDS             complement(3132..3365)
                     /locus_tag="KB942_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBB6851508.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3380..4330)
                     /locus_tag="KB942_001065"
     CDS             complement(3380..4330)
                     /locus_tag="KB942_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6851509.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4327..5169)
                     /locus_tag="KB942_001066"
     CDS             complement(4327..5169)
                     /locus_tag="KB942_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6851510.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5171..5890)
                     /locus_tag="KB942_001067"
     CDS             complement(5171..5890)
                     /locus_tag="KB942_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6851511.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6582..6740
                     /locus_tag="KB942_001068"
     CDS             6582..6740
                     /locus_tag="KB942_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851512.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6812..8038
                     /locus_tag="KB942_001069"
     CDS             6812..8038
                     /locus_tag="KB942_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6851513.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8126..8518)
                     /gene="rpsI"
                     /locus_tag="KB942_001070"
     CDS             complement(8126..8518)
                     /gene="rpsI"
                     /locus_tag="KB942_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6851514.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8534..8977)
                     /gene="rplM"
                     /locus_tag="KB942_001071"
     CDS             complement(8534..8977)
                     /gene="rplM"
                     /locus_tag="KB942_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBB6851515.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9496..9963)
                     /locus_tag="KB942_001072"
     CDS             complement(9496..9963)
                     /locus_tag="KB942_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBB6851516.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10083..11621)
                     /locus_tag="KB942_001073"
     CDS             complement(10083..11621)
                     /locus_tag="KB942_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBB6851517.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11701..12387
                     /locus_tag="KB942_001074"
     CDS             11701..12387
                     /locus_tag="KB942_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBB6851518.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12588..13115)
                     /gene="msrA"
                     /locus_tag="KB942_001075"
     CDS             complement(12588..13115)
                     /gene="msrA"
                     /locus_tag="KB942_001075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6851519.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13199..13876)
                     /gene="pgmB"
                     /locus_tag="KB942_001076"
     CDS             complement(13199..13876)
                     /gene="pgmB"
                     /locus_tag="KB942_001076"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6851520.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13873..15003)
                     /locus_tag="KB942_001077"
     CDS             complement(13873..15003)
                     /locus_tag="KB942_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6851521.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15003..17186)
                     /locus_tag="KB942_001078"
     CDS             complement(15003..17186)
                     /locus_tag="KB942_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6851522.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17183..18208)
                     /locus_tag="KB942_001079"
     CDS             complement(17183..18208)
                     /locus_tag="KB942_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6851523.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18253..19080)
                     /locus_tag="KB942_001080"
     CDS             complement(18253..19080)
                     /locus_tag="KB942_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6851524.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19077..20144)
                     /locus_tag="KB942_001081"
     CDS             complement(19077..20144)
                     /locus_tag="KB942_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBB6851525.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20170..21006)
                     /locus_tag="KB942_001082"
     CDS             complement(20170..21006)
                     /locus_tag="KB942_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851526.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21003..21872)
                     /locus_tag="KB942_001083"
     CDS             complement(21003..21872)
                     /locus_tag="KB942_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851527.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21942..23225)
                     /locus_tag="KB942_001084"
     CDS             complement(21942..23225)
                     /locus_tag="KB942_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6851528.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23239..24924)
                     /locus_tag="KB942_001085"
     CDS             complement(23239..24924)
                     /locus_tag="KB942_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBB6851529.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25199..26224
                     /locus_tag="KB942_001086"
     CDS             25199..26224
                     /locus_tag="KB942_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6851530.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8029 a         5396 c         4460 g         8879 t
ORIGIN      
        1 ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt
       61 tttttattcc accatttttt aaataaataa ttctattgca tgttttataa agaaattccg
      121 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
      181 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
      241 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
      301 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
      361 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
      421 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
      481 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
      541 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
      601 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
      661 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
      721 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
      781 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
      841 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
      901 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
      961 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
     1021 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
     1081 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
     1141 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
     1201 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
     1261 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
     1321 tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
     1381 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
     1441 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
     1501 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
     1561 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
     1621 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
     1681 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
     1741 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
     1801 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
     1861 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
     1921 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
     1981 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
     2041 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
     2101 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
     2161 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
     2221 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
     2281 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
     2341 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
     2401 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
     2461 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
     2521 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
     2581 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
     2641 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
     2701 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
     2761 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
     2821 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
     2881 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
     2941 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
     3001 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
     3061 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
     3121 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
     3181 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
     3241 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
     3301 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
     3361 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
     3421 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
     3481 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
     3541 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
     3601 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
     3661 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
     3721 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
     3781 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
     3841 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
     3901 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
     3961 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
     4021 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
     4081 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
     4141 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
     4201 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
     4261 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
     4321 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
     4381 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
     4441 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
     4501 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
     4561 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
     4621 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
     4681 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
     4741 cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa
     4801 aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa
     4861 aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat tgctttttat
     4921 aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat
     4981 acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat
     5041 aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac
     5101 tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt
     5161 ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat
     5221 tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa
     5281 taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt
     5341 ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta
     5401 tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta
     5461 aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc
     5521 ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt
     5581 gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact tttattggaa
     5641 aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga
     5701 tatcttcatt attaatggag acctctccct gatgagggat aatatttaac attgctttaa
     5761 gcaatgtgga ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta
     5821 tttttaaaga aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca
     5881 cttctaacat aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa
     5941 aaattatatt ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat
     6001 tagaatgaca aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa
     6061 aaggagcgtt ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat
     6121 tatacttgaa gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat
     6181 tattcaagac tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt
     6241 ttttcgtagt gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac
     6301 tatttttgat cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct
     6361 agaaactcaa gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc
     6421 tgggaaaaca aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg
     6481 taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa
     6541 ctactatttt aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct
     6601 gtgcatttct aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc
     6661 cagtaataag agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga
     6721 atacatttaa aataacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat
     6781 actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata
     6841 taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa
     6901 gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa
     6961 gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat
     7021 aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat
     7081 tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt
     7141 gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat
     7201 aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg
     7261 atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac
     7321 tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat
     7381 acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa
     7441 tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta tgattaccct
     7501 gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga
     7561 aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa
     7621 aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg
     7681 gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta
     7741 tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct
     7801 aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca
     7861 caaactccgt catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat
     7921 ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga
     7981 tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata
     8041 tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc
     8101 cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt
     8161 tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
     8221 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
     8281 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
     8341 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
     8401 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
     8461 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
     8521 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
     8581 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
     8641 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
     8701 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
     8761 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
     8821 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
     8881 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
     8941 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
     9001 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
     9061 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
     9121 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
     9181 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
     9241 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
     9301 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
     9361 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
     9421 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
     9481 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
     9541 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
     9601 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
     9661 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
     9721 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
     9781 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
     9841 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
     9901 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
     9961 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
    10021 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
    10081 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
    10141 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
    10201 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
    10261 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
    10321 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
    10381 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
    10441 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
    10501 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
    10561 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
    10621 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
    10681 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
    10741 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
    10801 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
    10861 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
    10921 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
    10981 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
    11041 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
    11101 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
    11161 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
    11221 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
    11281 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
    11341 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
    11401 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
    11461 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
    11521 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
    11581 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
    11641 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
    11701 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
    11761 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
    11821 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
    11881 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
    11941 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
    12001 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
    12061 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
    12121 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
    12181 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
    12241 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
    12301 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
    12361 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
    12421 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
    12481 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
    12541 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
    12601 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
    12661 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    12721 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    12781 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    12841 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    12901 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    12961 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    13021 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    13081 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    13141 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    13201 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    13261 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    13321 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    13381 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    13441 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    13501 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    13561 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    13621 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    13681 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    13741 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    13801 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    13861 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    13921 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    13981 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    14041 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    14101 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    14161 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
    14221 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
    14281 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
    14341 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
    14401 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
    14461 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
    14521 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
    14581 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
    14641 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
    14701 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
    14761 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
    14821 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
    14881 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
    14941 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
    15001 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
    15061 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
    15121 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
    15181 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
    15241 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
    15301 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
    15361 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
    15421 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
    15481 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
    15541 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
    15601 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
    15661 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
    15721 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
    15781 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
    15841 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
    15901 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
    15961 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
    16021 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
    16081 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
    16141 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
    16201 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
    16261 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
    16321 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
    16381 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
    16441 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
    16501 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
    16561 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
    16621 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
    16681 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
    16741 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
    16801 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
    16861 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
    16921 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
    16981 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
    17041 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
    17101 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
    17161 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
    17221 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
    17281 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
    17341 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
    17401 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
    17461 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
    17521 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
    17581 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
    17641 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
    17701 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
    17761 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
    17821 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
    17881 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
    17941 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
    18001 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
    18061 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
    18121 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
    18181 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
    18241 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
    18301 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
    18361 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
    18421 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
    18481 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
    18541 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
    18601 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
    18661 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
    18721 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
    18781 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
    18841 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
    18901 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
    18961 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
    19021 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
    19081 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
    19141 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
    19201 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
    19261 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
    19321 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
    19381 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    19441 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    19501 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    19561 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    19621 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    19681 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    19741 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    19801 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    19861 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    19921 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    19981 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    20041 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    20101 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    20161 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    20221 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    20281 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    20341 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    20401 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    20461 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    20521 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    20581 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    20641 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    20701 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    20761 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    20821 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    20881 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    20941 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    21001 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    21061 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    21121 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    21181 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    21241 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    21301 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    21361 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    21421 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    21481 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    21541 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    21601 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    21661 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    21721 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    21781 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    21841 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    21901 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    21961 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    22021 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    22081 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    22141 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    22201 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    22261 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    22321 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    22381 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    22441 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    22501 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    22561 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    22621 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    22681 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    22741 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    22801 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    22861 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    22921 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    22981 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    23041 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    23101 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    23161 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    23221 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    23281 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    23341 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    23401 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    23461 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    23521 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    23581 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    23641 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    23701 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    23761 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    23821 gtagtttact cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    23881 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    23941 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    24001 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    24061 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    24121 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    24181 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    24241 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    24301 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    24361 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    24421 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    24481 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    24541 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    24601 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    24661 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    24721 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    24781 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    24841 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    24901 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    24961 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    25021 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    25081 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    25141 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    25201 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    25261 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    25321 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    25381 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    25441 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    25501 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    25561 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    25621 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    25681 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    25741 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    25801 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    25861 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    25921 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    25981 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    26041 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    26101 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    26161 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    26221 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    26281 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    26341 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    26401 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    26461 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    26521 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    26581 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    26641 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    26701 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    26761 agac
//
LOCUS       DADVXN010000030        25757 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.031, whole genome shotgun sequence.
ACCESSION   DADVXN010000030 DADVXN010000000
VERSION     DADVXN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.031"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(169..333)
                     /locus_tag="KB942_001087"
     CDS             complement(169..333)
                     /locus_tag="KB942_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851531.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            628..1956
                     /locus_tag="KB942_001088"
     CDS             628..1956
                     /locus_tag="KB942_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBB6851532.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2158..2991
                     /locus_tag="KB942_001089"
     CDS             2158..2991
                     /locus_tag="KB942_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBB6851533.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3007..3744
                     /locus_tag="KB942_001090"
     CDS             3007..3744
                     /locus_tag="KB942_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBB6851534.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3899..4867)
                     /locus_tag="KB942_001091"
     CDS             complement(3899..4867)
                     /locus_tag="KB942_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBB6851535.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5099..5932
                     /locus_tag="KB942_001092"
     CDS             5099..5932
                     /locus_tag="KB942_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBB6851536.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5966..6805
                     /locus_tag="KB942_001093"
     CDS             5966..6805
                     /locus_tag="KB942_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6851537.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6831..7391
                     /locus_tag="KB942_001094"
     CDS             6831..7391
                     /locus_tag="KB942_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6851538.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7590..9311
                     /locus_tag="KB942_001095"
     CDS             7590..9311
                     /locus_tag="KB942_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBB6851539.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9476..10618
                     /locus_tag="KB942_001096"
     CDS             9476..10618
                     /locus_tag="KB942_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6851540.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10634..11845
                     /gene="thiI"
                     /locus_tag="KB942_001097"
     CDS             10634..11845
                     /gene="thiI"
                     /locus_tag="KB942_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBB6851541.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12418..13065
                     /locus_tag="KB942_001098"
     CDS             12418..13065
                     /locus_tag="KB942_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6851542.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13194..13410
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13458..16103
                     /locus_tag="KB942_001099"
     CDS             13458..16103
                     /locus_tag="KB942_001099"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBB6851543.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16413..17726
                     /locus_tag="KB942_001100"
     CDS             16413..17726
                     /locus_tag="KB942_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBB6851544.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17716..18369
                     /locus_tag="KB942_001101"
     CDS             17716..18369
                     /locus_tag="KB942_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6851545.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18424..19116
                     /gene="radC"
                     /locus_tag="KB942_001102"
     CDS             18424..19116
                     /gene="radC"
                     /locus_tag="KB942_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBB6851546.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19266..19466
                     /locus_tag="KB942_001103"
     CDS             19266..19466
                     /locus_tag="KB942_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6851547.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19720..21399)
                     /locus_tag="KB942_001104"
     CDS             complement(19720..21399)
                     /locus_tag="KB942_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6851548.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21401..21613)
                     /locus_tag="KB942_001105"
     CDS             complement(21401..21613)
                     /locus_tag="KB942_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBB6851549.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22006..23736
                     /gene="efrA"
                     /locus_tag="KB942_001106"
     CDS             22006..23736
                     /gene="efrA"
                     /locus_tag="KB942_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBB6851550.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23733..25493
                     /gene="efrB"
                     /locus_tag="KB942_001107"
     CDS             23733..25493
                     /gene="efrB"
                     /locus_tag="KB942_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBB6851551.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25588..>25757
                     /locus_tag="KB942_001108"
     CDS             25588..>25757
                     /locus_tag="KB942_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851552.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKV"
BASE COUNT         8446 a         4409 c         5450 g         7452 t
ORIGIN      
        1 gatgtcgtag gttcgagtcc tactgccgga gtaaataaag cttatgcaaa gaaaaagaaa
       61 tccaatcact tgggtttctt tttcttttat tattatgatg aatcaagctt tctgtttttg
      121 tcacgtatgt tgtaatcttt acgaaagagt agagttggct actcataatc aaccatactt
      181 cccatcatcg ctaaatacga aataaatcag cattcctaca ccaatcaaag cacaagctgc
      241 accaagaaaa gggatgacat tcccaaagtt atctgtattc ataagtatgc caccacccac
      301 aaacaaaaag ccgccagcaa tcaggttttt cattttattc atctcctttc ttacgttata
      361 gtatttcctt ctttcaatga gtattttgaa agctatctta ttgtatgatg ttcttctagg
      421 cctcttcttt attatacagg ccattttcgt tatctcatag aaatattcct ttttatctga
      481 aaactattgt ataccgtatg acatttttct tcatgaagtt gtaagtatat tctcattcaa
      541 atgaaaattt catcatagaa gagattgtat ttctaaattc ttaagagtaa aatacgaagt
      601 ataacttgga tttggaagga tgattggatg aaagctatta cgcctgatat gacaacaaaa
      661 tttaacaaac agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc agttgtgaag
      721 aacggaatta ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac accggtattt
      781 tctatagatt tggctacggg gaaagtggct aatcaaatgc aatcggggcg ttgctggatg
      841 tttgctgcac tgaacacatt ccgtcataag atgctgaatc aattccattt aaaagagttt
      901 gaattatcac aaaattacac ttttttctgg gataaatatg aaaaggcaaa ttatttctat
      961 gaaaacatca ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc tttcttactc
     1021 gctactcctc aacaagatgg cggccaatgg gatatgatcg tttcattatt ccaaaaatat
     1081 ggcgttgtgc ctaaatcggt gatgccggaa agtgcgaata gttcaaattc tcgagatttg
     1141 aataactact tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa aatggttgct
     1201 caaggagaaa cattagacgc tattgaagaa aagaaagaag aaatgctaga aagcatttat
     1261 aacttcttag ctatctcatt aggaacacct ccaaaagaat tcgattttga gtaccgcgat
     1321 gaagaaaaga actatcatct tgaccgagga ctgacaccac aaatttttta tgataaatac
     1381 atcggtgtaa aattagatga ttatgttagc gtgatcaatg caccaacaaa agacaaacca
     1441 ttcaatcgtt cttatacagt agagatgcta ggaaatgtcg ttggcgggaa agaagtcaaa
     1501 tatctgaatg ttgatatgga aactttcaaa aaattagcga ttgctcagct tgaagaagga
     1561 gaatctgttt ggtttggttg tgatgtaggt caatcttcta ctagaactag tggcatcatg
     1621 gccttagatg cttattcaat ggatgatcta tttgatacgg attttacaat gactaaagct
     1681 gaacgtttag attttggaga aagtctgatg acccatgcga tggtattgac cggtgttgac
     1741 ttagtagacg ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga aaaagttgga
     1801 aagaatggct tctttgttat gagtgatgcc tggatggacg aatatacgta ccaaatcgtt
     1861 gtaagaaaag atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga accaattgtt
     1921 ctatctccgt gggatccaat gggtgcttta gcatagacga gtatttcaat gaagagtatg
     1981 tagtaaagat aaaaagagct ggcttaagcc agctcttttt atctttaaag tccattccct
     2041 gttttttatg gtccctgtgc tataattcta aataaaggat aaggattgga aataatcctt
     2101 ttatcattaa aaatatgatg acgaagtaga ataaaattgg gagtaggggg gaaagtcatg
     2161 gatatccaaa ctgttttatc tgatctgaaa ttaaatggta aagtgattcc agttgttggt
     2221 ggcgatgtta atcagaccta tcgtataaaa accgaacatc gtgcttattt tttaaaaatc
     2281 catccgaatg taaaaaaagg attttttgaa gcagaagtag acggattaaa agaactgtca
     2341 gcattcgtac gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg cgcttatttg
     2401 ttaatggaat ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc agctttagct
     2461 aatctccatc agcaaacagc gccacaattt ggttttcgta aagataacta tttgggtaca
     2521 cttgttcaga aaaattcttt tgaagaagat tggtggactt ttttctttaa agatcggctt
     2581 gaatcccaga tttctttggc agaagaaacg aatcgatgga atgtacagcg tcaagaaaag
     2641 tatctgcgat ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat cactccacgc
     2701 ctcttgcacg gagacctgtg gagtggtaat gtgttctttg accagcaagg gcatccagtt
     2761 tttgtcgacc cagctgtttc ttatgggaat cgggaacaag atattgcaat gagccagtta
     2821 ttcggtggtt tccgaccgga atttttagat gcttatcaaa ctatatttcc attagaaaag
     2881 ggatggaaag accgactgcc gatttaccag ttatattatc ttttggcaca tttgaatatg
     2941 tttggagaaa gttacggttc ccaagtagat caattactag aaaattttta agaggagggc
     3001 gtgtttttgg caaaaaaata taccaaaaag catgaagttt catattatga gtgtgacatc
     3061 aaccaaacga tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc tgaagatcaa
     3121 agcgaccagt tgggaagagg cagtgaattc gtcaatagct ttggacttac ttgggtcatc
     3181 acgaattatt ccatggagat taatcgtctc cctagagtcg gtgaagtaat ctcagtaacg
     3241 actcaagcca tggagtacaa taagtttttc tgttatcgga acttctggat ccatgatgaa
     3301 gatgggaaag aattagtcaa aatagaatct gttttcgtat tgatggattt tgtgaatcga
     3361 aaaatgagca gtgttaatga agagatcatt gctccttttg agagtgagaa gatcaagaaa
     3421 atcaagcgtc aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc atatcgtgtt
     3481 cgtttttatg atatcgatag taatcagcat gttaataatg cgatgtattt taattggatc
     3541 attgacgtat taggatatga ctttttgact acgcatgttc cagaaaaagt aattattcga
     3601 tttgataaag aagtagagta cgggaatgag atcgaaagcc attttgaaca aatccctatc
     3661 gaaaatggtg tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta ttgtgaagca
     3721 aatattatat ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca acttcaagaa
     3781 ataagaagaa atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc
     3841 gaagaagcta tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt
     3901 agttttggat aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata
     3961 gttttaaacg tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta
     4021 gttcttttcc cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag
     4081 aaacaagtac tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg
     4141 gagggagatt ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga
     4201 tggcttccaa taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat
     4261 ctacagcgga aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat
     4321 tcttagttac taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg
     4381 tatgggtctt tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc
     4441 ccttatctag cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc
     4501 cgtcacttcc aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt
     4561 tgccaatcgt tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga
     4621 tcaccactcc ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca
     4681 tgtgagaagg catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta
     4741 catgcttggg gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac
     4801 cgcccgtgag catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt
     4861 ttttcatttt acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata
     4921 ctatataaaa gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa
     4981 agctgacctt attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct
     5041 ggttgagttt cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat
     5101 gatcagaatc ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa
     5161 cactctttat gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg
     5221 aattccccga aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt
     5281 tgtagtcaaa gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac
     5341 cgaagaagag atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa
     5401 gatgttttgc tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca
     5461 gtacttgcgc aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga
     5521 ttattcttgg tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc
     5581 tttttcttta gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc
     5641 atttgtcacc aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg
     5701 gcaagcaaat gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga
     5761 actgcaagaa ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt
     5821 gatttttaat aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat
     5881 attaaatatc aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat
     5941 tttattcaag aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac
     6001 attggtcacc aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg
     6061 gaaaaacggc attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat
     6121 cattgatgaa ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc
     6181 agatcgcacg attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt
     6241 tgcagatgag aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac
     6301 gacgatgcta ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa
     6361 attcccggta cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga
     6421 tcgttatgaa acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc
     6481 ggagtcagaa cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa
     6541 tacgtatact gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt
     6601 cgcaaatgct gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga
     6661 tatcgagatg ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt
     6721 aaaacatgct gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact
     6781 gaaacacttc ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag
     6841 agacaccttt tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga
     6901 tacccacggc ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac
     6961 ttgttgaatt tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc
     7021 tgctgcacga agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa
     7081 gtgatccgct ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt
     7141 ctttttcatt actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata
     7201 tggggaaatt atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga
     7261 aacctgaaaa ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac
     7321 gtcagattca agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg
     7381 aaaaagagtg atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga
     7441 ttttcgttta agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt
     7501 ttgcacgaga gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa
     7561 gatgcatact gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca
     7621 ttgcaatcat tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa
     7681 accaagatcg attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga
     7741 ttgaagaagt cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc
     7801 gtgaatggag tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc
     7861 aaattttcga agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag
     7921 ttgttgaagc agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag
     7981 gtctaaagga attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg
     8041 acgtctatga agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc
     8101 tttttgaatt gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt
     8161 taaacacttc tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata
     8221 cgtatgaagt ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca
     8281 ctttccctga acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat
     8341 atgtattccc agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa
     8401 attcattagc caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa
     8461 cagctgattt gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat
     8521 atgtaaaaac taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc
     8581 gtcaattatt gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa
     8641 ttggccgtgt acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga
     8701 tgattccaca gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa
     8761 cagtcttcaa agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac
     8821 atgaattgag aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat
     8881 tgcgcagttt gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc
     8941 tagaattctt ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa
     9001 ttcgagtaaa catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat
     9061 tattagataa gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc
     9121 aatatgcaaa tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct
     9181 actatttatt caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat
     9241 tagagcgagt agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag
     9301 acttagtata aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt
     9361 tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa
     9421 agcaccttgg acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat
     9481 atattttgat aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa
     9541 aaccagtcag cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa
     9601 ccggttgttg cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga
     9661 aatctatttt acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat
     9721 cgaaaaaaga gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag
     9781 cgagacagca gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa
     9841 acgcggattt gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt
     9901 ctctgttatg gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga
     9961 tcttttggat gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa
    10021 agtaccattc tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa
    10081 attccatggc ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc
    10141 attactgaca ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg
    10201 tattgtggct atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga
    10261 gcagacacaa cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac
    10321 gattttttca gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat
    10381 tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag
    10441 tgcctgctcc tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa
    10501 tgatctggct acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt
    10561 cgagcaattt atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa
    10621 gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa
    10681 aggaaagaat cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa
    10741 agactttcca gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg
    10801 cgcagacggg cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc
    10861 tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat
    10921 gaaagaaatt tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca
    10981 atttgaattg acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt
    11041 cccgcatatc caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg
    11101 tgatggcgct tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac
    11161 aagcggaaaa ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt
    11221 tgccatgaag cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag
    11281 tgaacaagca ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac
    11341 gatccatttt atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc
    11401 acaaggatat tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg
    11461 ccgtattcgt catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca
    11521 aactttgcaa agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt
    11581 agcgacaatg gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct
    11641 ttctatccag ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg
    11701 ccctcaactg gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca
    11761 acgagcacta gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac
    11821 tgaagaattt tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta
    11881 gagaaacaaa acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga
    11941 ttagctgatt gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac
    12001 tgaaaaataa aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga
    12061 atttcccatg aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa
    12121 tttacgaaaa ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac
    12181 ttctggaaca ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag taataagaac
    12241 agccaaacaa aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca
    12301 agcttttgaa caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct
    12361 cccgtgttag aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg
    12421 cgagaaaaaa cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc
    12481 tacttgaagt tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa
    12541 atcacccaaa ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc
    12601 agaagcggat atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat
    12661 actaaagaag aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa
    12721 cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca
    12781 gcactgatca atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag
    12841 atagccaaag caaaaaatgt gacagtagca atctctacgg taccaagtaa atattcacaa
    12901 tctgctattg atgaaattgt caaaggaaat gtaaccgcta tacttaattt tgcgcctgac
    12961 agagtaactg tcccagctta tgtcaatgtt caatacattg atttgacaac cgagttgcag
    13021 acgcttattt actttgatga gaattactca gaagtttttt cttaggaaaa tttgagtagg
    13081 cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt actaaatgaa
    13141 gtggtttttt cacgaccact tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa
    13201 cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat ctgctgaaag
    13261 gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg gagtagagat
    13321 agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat tgcaatttag
    13381 gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg ttttttagtt
    13441 taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat aatccgcaag
    13501 aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa ccgagcggag
    13561 ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc
    13621 atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa aagagaatgc
    13681 aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg acacaagcaa
    13741 aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt
    13801 tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc gaacagtggg
    13861 ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat gatggattat
    13921 ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt tatcgtggtg
    13981 aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc gaagttattc
    14041 ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat ggatcaggcg
    14101 tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg attgctgttc
    14161 atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta cctttagtca
    14221 ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg acaggtgtag
    14281 taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt catgatcttc
    14341 cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga aaatatgctg
    14401 gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat
    14461 tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca ggtgtcgttg
    14521 ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg aaaaaagcca
    14581 ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc aatcaaacgt
    14641 ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta tggtggggac
    14701 atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg atggaagcac
    14761 cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg tttagttcag
    14821 ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat tataagcgat
    14881 acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg gtaagcagaa
    14941 tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc ttgattcatg
    15001 gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat ggaatcgatc
    15061 cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta tcgaatggtt
    15121 ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct tggaacttta
    15181 tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag
    15241 aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt actcgtttga
    15301 atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt gaagcaggtc
    15361 gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa atgagtaaag
    15421 aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc ttagtccata
    15481 cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca gaagaaatat
    15541 gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct gttgtacgtc
    15601 cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa ttgattcgag
    15661 ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt acgttgctaa
    15721 ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc tatatcgaac
    15781 gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca gatctagcga
    15841 tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg atcaatattg
    15901 aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg
    15961 tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa gtagtagagg
    16021 cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa gaacgaatcg
    16081 aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag ctagctatta
    16141 ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac tcttagcttc
    16201 gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct actcttatta
    16261 tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt tgataaaatc
    16321 gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg tgttctattt
    16381 gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca atagaatgga
    16441 tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa gctttattag
    16501 aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga acaaacggaa
    16561 aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg acagtaggta
    16621 catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat ggcaaaagca
    16681 ttccagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc aaacaacttg
    16741 atcaatttga agcagtagct ggtattactg aattcgagac attaacagca atggcatttg
    16801 attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta ggcggcttat
    16861 tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt ggacttgatc
    16921 ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct ggaatcatca
    16981 aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact gtcatccaac
    17041 aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat aaaataaact
    17101 atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa ataggaaaga
    17161 tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca ggagtagcca
    17221 tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt
    17281 tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt aacgagccat
    17341 tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt gagaatttga
    17401 aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga actaaaaatg
    17461 ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat ttgacgacct
    17521 ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt gagagagtat
    17581 caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg acaaatgaag
    17641 acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat ttattaatac
    17701 aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg tttgctgttc
    17761 gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat gggttttcct
    17821 tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt atgggcgaat
    17881 tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat agatgattca
    17941 taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg
    18001 gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc cagtaaccaa
    18061 cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt tgagacgatt
    18121 gtttctgcag aaaatgttca acgagcaaaa ccagacccag aaattttctt gttagctaat
    18181 cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca aaatggtatt
    18241 ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct tccaccatcc
    18301 caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat ccctgcattg
    18361 ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa aggagatgtt
    18421 tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag ggaacgtatg
    18481 gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt actgcgtacc
    18541 ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac gtttggagga
    18601 ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg gataggacgc
    18661 gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca gacggatcat
    18721 gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt gattctggaa
    18781 atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa gaatcaagtg
    18841 atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca tcctagagaa
    18901 attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca taatcatcct
    18961 tctggtaatg ttattccatc gcagcaggat atgaatttta ccaaaaggat acaaaagtgt
    19021 ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa acgatatttt
    19081 agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca aaaaaaatga
    19141 agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac atctgttgca
    19201 cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac gaggaggata
    19261 ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt ggatttatct
    19321 cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt gacggattca
    19381 aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac cgcggcccac
    19441 aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg gtagacatca
    19501 atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac aatttctctt
    19561 gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg aaagagccac
    19621 cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat ttgttttctc
    19681 ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat ctggtgtcat
    19741 taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag gctgaagcgc
    19801 gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca aacattctct
    19861 taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt ctcgcatgta
    19921 gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct tgatatccac
    19981 tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac
    20041 attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca caaaatgccc
    20101 agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca tgatgaagca
    20161 gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca acatgcggaa
    20221 ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta atttttgttc
    20281 ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa tcacttctgc
    20341 accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac caggaatagg
    20401 tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt gagtaccatt
    20461 cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata ggatcatcgt
    20521 ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt
    20581 gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc
    20641 gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga agatgttaga
    20701 agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca aaatagaatg
    20761 cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac tatcagataa
    20821 aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg ctggttcgcc
    20881 aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag aaggtgtttt
    20941 caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt
    21001 ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat ctcgaagcaa
    21061 gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag catagatagg
    21121 aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt gcccgtgagt
    21181 aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac
    21241 atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat
    21301 ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc caccaatagc
    21361 aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc actaatgaga
    21421 aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga acgaattcaa
    21481 tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa
    21541 tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct tgataataaa
    21601 ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta gctagttgat
    21661 ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg ctttgactag
    21721 aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc ggatattttt
    21781 tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta gcataaaaca
    21841 gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat catcccgcta
    21901 taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt gtgctatcat
    21961 ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa actaatgtgg
    22021 cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt atttggtttt
    22081 attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt aggaatccgc
    22141 aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat tactgtaatc
    22201 ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac taatatcgta
    22261 gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca ggaatacgaa
    22321 aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca gatcatgttg
    22381 ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat tgtcagtttg
    22441 tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc attaccttta
    22501 ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa aaagcaacaa
    22561 aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt
    22621 cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat
    22681 gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg tttttatttc
    22741 ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat cgatgcaggc
    22801 gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc cttgttctca
    22861 ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc agctggacgt
    22921 atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg tatatctgat
    22981 acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc aggccattcg
    23041 caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac cgttgctttt
    23101 attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg cttttatgat
    23161 gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg gctaagcaag
    23221 ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg aacgattgca
    23281 gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg tgcagctgag
    23341 attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga acatttatca
    23401 gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc cagagccatc
    23461 atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga ttatcaaacg
    23521 gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt attgatcgta
    23581 gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa tgaaggagaa
    23641 gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta ttatgatatt
    23701 gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct tccttaaaac
    23761 gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc ctatttacag
    23821 ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct actacagaaa
    23881 tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc aaatgtctca
    23941 tttggatcac agttgttgga atagggtatt gtatttcgca attactttct ggtgttttga
    24001 tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa gaaaaaatca
    24061 atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg tctcgtgtga
    24121 cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt atcgtaaatg
    24181 cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg atgatggcgc
    24241 tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg atcaatgctt
    24301 cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc tacgtccaag
    24361 aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa acattggaag
    24421 gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca ttcatttctg
    24481 gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt atggctgttc
    24541 taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa gcattcatcc
    24601 aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct tcagttttgc
    24661 aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca gaagaaaaag
    24721 aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca ttcgatcatg
    24781 tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt gaagtgaaag
    24841 ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca ttgatcaatc
    24901 tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga atcgacacca
    24961 aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc
    25021 tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg acagattatg
    25081 aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg atgccagatg
    25141 gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa aaacagttgc
    25201 tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat gaagcaacaa
    25261 gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg
    25321 gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca gatctgattc
    25381 tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta ttggcacaag
    25441 gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa taagtcatat
    25501 aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg gtaaagtaaa
    25561 gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa aattactgaa
    25621 gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt tgattcagct
    25681 gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa
    25741 ttaattttaa aaaaagt
//
LOCUS       DADVXN010000031        25456 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.032, whole genome shotgun sequence.
ACCESSION   DADVXN010000031 DADVXN010000000
VERSION     DADVXN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.032"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(87..497)
                     /locus_tag="KB942_001109"
     CDS             complement(87..497)
                     /locus_tag="KB942_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851553.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            772..984
                     /locus_tag="KB942_001110"
     CDS             772..984
                     /locus_tag="KB942_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851554.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            987..1199
                     /locus_tag="KB942_001111"
     CDS             987..1199
                     /locus_tag="KB942_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851555.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1339..1494
                     /locus_tag="KB942_001112"
     CDS             1339..1494
                     /locus_tag="KB942_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851556.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1491..1715)
                     /locus_tag="KB942_001113"
     CDS             complement(1491..1715)
                     /locus_tag="KB942_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6851557.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1793..1981
                     /locus_tag="KB942_001114"
     CDS             1793..1981
                     /locus_tag="KB942_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851558.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2307..2642
                     /locus_tag="KB942_001115"
     CDS             2307..2642
                     /locus_tag="KB942_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851559.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2875..3816
                     /locus_tag="KB942_001116"
     CDS             2875..3816
                     /locus_tag="KB942_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBB6851560.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3818..4708
                     /locus_tag="KB942_001117"
     CDS             3818..4708
                     /locus_tag="KB942_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6851561.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4751..5551
                     /locus_tag="KB942_001118"
     CDS             4751..5551
                     /locus_tag="KB942_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBB6851562.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5566..6417
                     /locus_tag="KB942_001119"
     CDS             5566..6417
                     /locus_tag="KB942_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6851563.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6618..7079
                     /locus_tag="KB942_001120"
     CDS             6618..7079
                     /locus_tag="KB942_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6851564.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7105..7557
                     /locus_tag="KB942_001121"
     CDS             7105..7557
                     /locus_tag="KB942_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851565.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7663..7857
                     /locus_tag="KB942_001122"
     CDS             7663..7857
                     /locus_tag="KB942_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851566.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7888..8274
                     /locus_tag="KB942_001123"
     CDS             7888..8274
                     /locus_tag="KB942_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851567.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8271..8474
                     /locus_tag="KB942_001124"
     CDS             8271..8474
                     /locus_tag="KB942_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6851568.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8471..8767
                     /locus_tag="KB942_001125"
     CDS             8471..8767
                     /locus_tag="KB942_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851569.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            8843..9259
                     /locus_tag="KB942_001126"
     CDS             8843..9259
                     /locus_tag="KB942_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6851570.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9520..9657
                     /locus_tag="KB942_001127"
     CDS             9520..9657
                     /locus_tag="KB942_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851571.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10067..10297
                     /locus_tag="KB942_001128"
     CDS             10067..10297
                     /locus_tag="KB942_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851572.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10456..11289
                     /locus_tag="KB942_001129"
     CDS             10456..11289
                     /locus_tag="KB942_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBB6851573.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11282..12697
                     /locus_tag="KB942_001130"
     CDS             11282..12697
                     /locus_tag="KB942_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBB6851574.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12709..14238
                     /locus_tag="KB942_001131"
     CDS             12709..14238
                     /locus_tag="KB942_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6851575.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14156..15208
                     /locus_tag="KB942_001132"
     CDS             14156..15208
                     /locus_tag="KB942_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBB6851576.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15210..15527
                     /locus_tag="KB942_001133"
     CDS             15210..15527
                     /locus_tag="KB942_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851577.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15654..16277
                     /locus_tag="KB942_001134"
     CDS             15654..16277
                     /locus_tag="KB942_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBB6851578.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16292..17182
                     /locus_tag="KB942_001135"
     CDS             16292..17182
                     /locus_tag="KB942_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBB6851579.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17205..17420
                     /locus_tag="KB942_001136"
     CDS             17205..17420
                     /locus_tag="KB942_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851580.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17432..17773
                     /locus_tag="KB942_001137"
     CDS             17432..17773
                     /locus_tag="KB942_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851581.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17773..18144
                     /locus_tag="KB942_001138"
     CDS             17773..18144
                     /locus_tag="KB942_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851582.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18137..18532
                     /locus_tag="KB942_001139"
     CDS             18137..18532
                     /locus_tag="KB942_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6851583.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18534..18911
                     /locus_tag="KB942_001140"
     CDS             18534..18911
                     /locus_tag="KB942_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851584.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18912..19520
                     /locus_tag="KB942_001141"
     CDS             18912..19520
                     /locus_tag="KB942_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6851585.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19520..19861
                     /locus_tag="KB942_001142"
     CDS             19520..19861
                     /locus_tag="KB942_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851586.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20103..22892
                     /locus_tag="KB942_001143"
     CDS             20103..22892
                     /locus_tag="KB942_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6851587.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            22882..23622
                     /locus_tag="KB942_001144"
     CDS             22882..23622
                     /locus_tag="KB942_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6851588.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            23619..>25456
                     /locus_tag="KB942_001145"
     CDS             23619..>25456
                     /locus_tag="KB942_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6851589.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9278 a         3621 c         5240 g         7317 t
ORIGIN      
        1 ttaatcgcat cagatccaaa ctctacaaat aatttttcta tttcttctaa tatccagtat
       61 ttgtttaaat gcataccaaa gcccacctat tgacgatatt ttttaggagt gaattttttc
      121 tttgctttaa ctttggttaa ggcaagtgca agttgaatgg catcgtataa tttttcctta
      181 tcttcatcgt ctaaaggttc accataataa tttgtttcag catctgaatt caagccttca
      241 agtaattttt cagcttgtaa agcaatatct tgtttatctt tttctgttaa atcataataa
      301 tggcgtttat cagtacgtcc taaaagataa tcagttgata cattaaagta atctgcaact
      361 ttttgtattt tatctccaga tggagtactt ttattccatc tagacatgct tccatttccg
      421 aaatttaact ttctttcaag ttctgccaac gtcattgaac gttcttttaa taagattttt
      481 atacggtcta atagcatagg atatgcattc ctttcaaaat accgacaaaa aaagtatttt
      541 tctgcaaatt atctatttac aaatagagaa tttgcagtta tactagtttt gtaagcaaga
      601 tatttacggc atacaaagac taaaagcaat aaactaatag tctccccaga catcagtaag
      661 ctcgcttttt gtgcgctgta ttttgtatgc ttacataata gcatatttgc agtttttgtc
      721 aaacttatta gacaaaaata ttagaaaata ttcgataagg gggtatgaag catgacttta
      781 aaacagcaag tattaattgc tttagtaaag aaaggttgga gtcaaagaga gttagctcga
      841 cgaatgggta tatcaattac ttatttacga gatatcttca tcgaaaaaag aaaacctaag
      901 gaacgtttaa aacaaatcga agaacttttg gatatcaagt tagaagtaga ttctaatgat
      961 cagccatcag aacaggaggc gtaaaaatgg tacaacgttt gagcaaagcg gagtttaaag
     1021 agaaatataa gtattctgaa agtacttatc aaagaagaat gaaggaattt aaaaaatcac
     1081 gttttagtga ggggtataac gcagttactt ccaaagaaat aacaattgat gtcgatttat
     1141 atgaacagtt tagaacatgg cgttctaaaa atcgatttag agttagagct gtttgttaat
     1201 agctaaaaaa tctaaattca ccacttctga aaagaggagt tgctgctctc ctttcaagtt
     1261 tgccaaagtc cacgtttttt cattttggca actcctcttc ttggaggtgg tgacggcgag
     1321 agacgaagga gggatgacat gaaggaagaa tcaaaaatag ggattttgaa tgagaacaat
     1381 tacacaagat taattattga aacagaagaa gggaaaaaag tagcagaagt tacattgacc
     1441 gatgcaactc ctgctgacgg ttatcgtatt cgattaactc ctaaatatga ttaacctttt
     1501 ggtgggtgtg gatcgtttcc gtgactatca cggctttgaa tttttccatc tctgccatga
     1561 ataaatagct cagatccttg atttttagaa atctctctag cgatttttac tgcttctgat
     1621 tttgtattag tatgtgcagt cgctttagaa tttccagcac ctttgacatt ccaaccacca
     1681 ttggaattag gtacaacatg ttgatttttt cccatagttt tcacctccat ttctatagaa
     1741 atattttatc aaatagaaat ggagaaattc aataaacgaa aggagaagtg atatggcata
     1801 taccttgcaa caagaacatc aaattctcgg tttgattaaa caacgcagga aacaattaca
     1861 agatgaccgt gcagcgctta gaaaagccga tgagctatca gataggcaag ctgaactaat
     1921 tgcttctgaa cttgaggatt tgagaatgct agaaataaaa aatagggaga ttagattatg
     1981 aagaagacag acacactttt tataggattc attttggggt tattagtgat tgtagcgcac
     2041 caaagtatta tcgggggaag cttgttcgca gcattgatgg ttttaatcaa tctgcttgat
     2101 tcaaaagaaa ggagcaacta tggcacgaga agaagcgcta aaaatcggta aagtgattgc
     2161 tgataatcgg tggggaaata gccgccctat tattttaagc aagcaacata tcgaaaagca
     2221 aaaagcatgg caacaaataa aaaagtgact ctgccagcca gcaaagagtc acatacaaat
     2281 ttataaaact aggagaatta tatcacatga ataagaaaat tgaaaatctt atcgaagaat
     2341 tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa aaagaagggg
     2401 aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt gctatgcaag
     2461 aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga attgctgtgg
     2521 atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat acttttgtta
     2581 ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg gaatttcaat
     2641 gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag tgattgtagc
     2701 gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt
     2761 agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac tatttaactg
     2821 atcctgatta tgctaaacca ccatatgaag caccaattga tgaggaggat gaagatgagt
     2881 aaatctacct tagaaatgag ccatcaagaa tggcttgaag accgtaagaa aggcatcgga
     2941 ggttcggatg ttggaacaat tttaggattg aataaatgga aatctcctta tcaactatgg
     3001 ctagaaaaaa caggacaagt agtacttgaa gaatcaggaa gtgagcccgc ttattggggt
     3061 aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc
     3121 agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa tattgatcgg
     3181 gaagtagtgg gagaaaatgc cattctggaa tgcaaaacag ctaatcaatt tctcggtaaa
     3241 gaatgggaag gcgaagaggt accgcttagc tatctctgtc aagttcaaca ttacatgaac
     3301 gttctaaaca aagactattg ttacatcgct gtcttaatcg gtggtcaaaa atttatctgg
     3361 aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt agtagagttt
     3421 tgggaaacaa acgttcttgg aggtatcgag ccaattattg atggtagtca agcgacagct
     3481 gatttcttga aagagaagta tgcagatgtt gaagacgttc aaacgacgtt accaattcat
     3541 ttcgatgaac tagttgaaca gaaaaacgaa ctcaaacgga ctaagaaaga aattgaatca
     3601 gctatccgtc aagtagacaa cgagattatc agcgagctag gtaaacgtga agccagtatc
     3661 ggtatcactc aaaaaaacat catcagttgg aaatttgtcc gtacgagacg tatgaactcg
     3721 aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta taacgttact
     3781 gaatcaagaa ggctaaccga aagggagatc aaataatatg gcaacaaatg aatcgttaaa
     3841 aaatcaattg gcagcaaagc cacagaaaca agttgcacca ggtcaattag gacttaaagc
     3901 tttgatgaat acaccaacaa tgagaaagaa gttcgaagag gtgcttcatg acaatgctaa
     3961 tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg cagatagtga
     4021 accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa
     4081 gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa caggaaaatg
     4141 ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag cccaacgatc
     4201 aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa aaagctggaa
     4261 ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg aagtcattgg
     4321 atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt ggaccaaaca
     4381 agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa agttaagtgg
     4441 tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa atcttctttc
     4501 taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg agagagtcca
     4561 aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag atattcctga
     4621 aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg gattatttga
     4681 tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct tttctagagg
     4741 agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga attcattcgt
     4801 ttggaccaat ccttatatgt ataaattatg gaacttgtgt ttgatgaaag cgagtcatga
     4861 gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag aattcgtcac
     4921 agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa
     4981 cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa
     5041 atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc aaagcagtga
     5101 acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa acaagaatga
     5161 aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac gcaaaaagcg
     5221 tgtttacgac accgactcag tttactacat tctcgcggag gagttattca aacagatttg
     5281 tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagataaca ttcggaaaat
     5341 gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat ggagtcagca
     5401 caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa aatacgacac
     5461 aatggcagcg caagccaatc gtgattataa aacaaaacaa actaaaacgc ttgaatacga
     5521 gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga aactgttggg
     5581 gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc
     5641 ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca
     5701 acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca aacagaacga
     5761 atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg ttctgttgtg
     5821 cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga tcaagaaaca
     5881 agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg aaatccaagt
     5941 caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat ggcagcggct
     6001 tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg gttacaggaa
     6061 atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac gattgaggga
     6121 tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat cggttcagaa
     6181 ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa ttcgttcttg
     6241 gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga agaactgaaa
     6301 aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg atttacgatg
     6361 gtattttctc aaacagcaga caagcgcata aaaccagtca aaggagcgat cgcatgaatt
     6421 tgactaaaca agaaaaaaga atctgtcgtc agataatgtt gcaaattata ggtgatcgtg
     6481 acatggacga attatctcaa caagagagtc gaatggttcg gaagataagt aagctaatta
     6541 tttatcggga tacagaaaga tttctcactg ttttaggtaa cgtctacgag aatccagagt
     6601 tattggaggt agagtaattg ccaaaaatat tagatgcatg ttgcggtagt agattgttct
     6661 ggtttgataa gaacaattct gacgtaacat ttatggattg tcgtcagcag tatgaagaat
     6721 tatcaacagg tcatgtgatc aatgttgatc cagatgtggt cgcagacttt cgcgatatgc
     6781 cttttgaaga tgatgtgttc gatatggttg tatttgaccc gccacactta agacatgtcg
     6841 gcgaaaactc atggcttgcc aaaaaatatg gaaaattaga cgaactttgg ccagaagaca
     6901 tcaggcaagg ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca ttaattttta
     6961 agtggaacga ggaacagata ccactttctg atgtgttaga ggctattgga gaacagccat
     7021 tgtttggtaa taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac
     7081 ggaggaaaca aaggaggaaa agtaatgaag attgtattaa ataaatgttt tggtggcttt
     7141 gggttgagcc atgaagcaaa aatggaaatt tttaaaagaa aaaatattga agtattccca
     7201 tacatcaata actttagcta tgactcagat gatgaataca cgagatacac aggccaaaag
     7261 ctaggttcga tggattttat ctactatttc aaaaaagacc cgaaaatcga caaagttaca
     7321 ggaatttata gcgaaattga ccgactgtac ggaattgctg atgattccag tttttcaagt
     7381 gattcaaaca gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc
     7441 agtggaccat atgcaagtct taaagtcgtt gatatacccg atggggctga gtgggaaata
     7501 agtgattatg acggcgttga aactgcacat tatggttttc aaacaggtag tatttagtcc
     7561 actaatgaca actaaagagg aggcagaagg atgagtgaag atgaatactt tgaacagtag
     7621 tctattaacg acagagattt ttaactggaa aggggtgtag tcatggacgc atcggaagta
     7681 gtaaataaaa ttaatagtct caaagaggtt ttcggagacg tcgaagtagt gataaacgca
     7741 caaggctatg acaacggatt ttttaagcag attaccgaca ttagcattca gcaaggaatg
     7801 gaagacgaag atggaaactt tatcgatgaa gtagccattt tagtgacttg cgagtaagta
     7861 attgacagct aaagaggagg cagaaggatg aaaaaattgc accaactcat atctgagaaa
     7921 gaaagcgaac tccaaaattt agaagatagt ttgggattgg ggtttccaat tgttgagcaa
     7981 gctaaaatga ctcaaatcag tcatctacga cttgaattgg aagatttaag acaaatagaa
     8041 aaatcaattc aactcaacga caaccagaaa atcgtgttgg agtggttgaa aagcgagaca
     8101 attttaacta gagaagcacc aatattatct gttaatgctt tttctgataa aaatttacta
     8161 ggaaaattac ctgataaagt acgcaaagct tataaactat tggcttgtaa acaagaatat
     8221 gaagtcctat cagcattcgc tcaatgggga ttagaacagg aggaagcgga atgaacaaca
     8281 gacatcgcag aataacaaaa ctaagaaaac aggaactgaa tgtactaaag acaaagtttg
     8341 aaaaagaata tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg
     8401 atgcgagtaa tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg
     8461 aggaaatgag atgaaaataa aagacggatt ttacgctagt agtcatggta tcggcggttt
     8521 aatgctagac atgccgataa agaaccctaa aacacgtcag aaatcaaaat tcaaaatcgg
     8581 tgacatggtc cgatgtaaag cagaagagtt catctatccg tttcgtggat atgtagagca
     8641 tgtctataat cattcagcaa tcattcacat tgaaaacacg atggaatgcg ataagtggtt
     8701 agcgaaaagc aaagataatt tagcagtggc tcgtttggtg gatatggaac taatcaatga
     8761 caaataaaaa agccggatcg ctccgactga ttcaataaat ccaacacatt tattatatca
     8821 cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca aacagacaag
     8881 atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg caggtcgctc
     8941 tttgatagat attaagtcgc cgattattac ggatatgccg aaggcaccga agcacggcaa
     9001 taaggcagaa gacgcgatca ttcagatgat ggatatagaa gcggagagag acgcaatact
     9061 agcggctttg atggctctta gtctgattag ccgtcagata ctctactaca gcttctgtga
     9121 cgtaaacaag cactctaatt atgaaatagg gcaattgata cgaggatacg gagagaagaa
     9181 tgtagagaag ttgaaatcca tcgcattaat cgaatttgca gaagcataca aaaaaggcgt
     9241 gttagttcag tatcgttgat tttgtagggt ttttgtaggg atagtgtagg gtttttgagc
     9301 ggtttaacgt gatattatgg tagtgtcgaa agattagtga taggtctgag acaaaataaa
     9361 atgtaaggga ggaaatctcc ctcatcgttt taacttaagc ttcgatagac agcaacggaa
     9421 atattaagaa taaggatgtg aatttcaact ccttctaaat tgttcttatt atctatcatc
     9481 cgttgttgtc tattaattta tgcatcggag gaaaaacgaa tggataaaga aatcaaagta
     9541 actgtcaaat tagatttgac tgaactaaaa gaactgctca acaaggctag tgaccaagtc
     9601 gaacagttac aagaaacttt agatgaaatt gctaatttta aaatccaagt ttcttagcag
     9661 tatatttttt agcagctgca ctcataaaat cataccaagt atcagaaaaa tataacttaa
     9721 ctaaataatt aaaaaaggtg ggtaaataga aatgacggcg ttaataattt caatttttgc
     9781 gctctgtctt aatgtctata tgattggatt taaaaatgga caaaataaaa aatagtagca
     9841 gccgagaata attttatagt gtcactgtgg cggaaataga agacgcaaag gtggaggtag
     9901 gaagcgtatg gcgcactatc gggaccttgt acaaaactag aaaggactag tacgtgtgtg
     9961 gtgcgagccc tagggaggaa caggataacc gcctgtgtgt aggttgctat tacatattag
    10021 atcactcttt gagtggtctt tttatttaca taaaggaggc tacataatga gaaactactg
    10081 gtatatatcg ctaactaatg aatatcctcg aaccattgat gattgttcag tgcgtgttgt
    10141 gcgttctgtc caaatcaaaa agaagtactc cattgttgaa atgaccagag aagctacgcc
    10201 aaaagagatt gataaatgca aacttcttta ttgcggtcat ggtttctatg atgaaccaaa
    10261 cattcaaaat aatattaaca agaatttgag ggattagaat gcaagaaatg gcttaaaaat
    10321 ctccaatcag gacaaatcaa gttagacaaa gtttccgatt taaagatatt gattgaagct
    10381 gatttaatgc taaaagatat tgaaaattag aaaacaaaac tcaacctaag aagattgcga
    10441 ggtggtgtgt attgaatggc aagacaacgt gatccaagac gtgatgaagc caaaagaatt
    10501 tggttagaat ccaacggaga aaagcagtta aaggaaattg catctgaatt aaatgtttca
    10561 gattctcagg ttagaaaatg gaaatcgcaa gacaaatgga gcgctgaatt gaaaagtaac
    10621 gttaccaatg gcaaaagtaa cgttactaat caaggtggcg ctcctattgg taatcaaaat
    10681 gctaaaggta acaaaggaaa cagcagagca tcaccgccaa agagaaataa gaatgctctt
    10741 aaaactggtg agtatgaaac aatatttttt gacacgttaa ccgataatga gaaggacatc
    10801 tattctagtt tgaatgatga tccttctttt gctttgtccg aagaaatacg acttcttaaa
    10861 ataagacaac ttcgaatgat gaaaagaatc caacaagctg aagctggact aaatgatgaa
    10921 gaagtcgaac gattgcagca gctaagaaag attaaaactc cgattgaaaa aaatggtaaa
    10981 aagctagaaa tcaagcgtga ggttatgcaa gatgtgcaga ttagcagaaa aaaacatcgc
    11041 aaaattgatg atattctttc aattgaagat tcattgactc ggattagcaa ccagttagct
    11101 aaagccatca agcaaatgaa tgaactttat atgaatgaat acagaactga tttaattaaa
    11161 gctcagactg ataagatcca agctgagaca aatgaaattg gcggaaataa ttcaggtgaa
    11221 gaaatagaag aatggaaaca ggcagtttta aatgccgcaa acaaacgggc ggtgaaagaa
    11281 aatgaataat gaatttattc cttttgctga tattggttct gccattgatt attactatga
    11341 taaaccagta gctttttgcc aagatatttt gcatttgaat cctgatgaat ggcaagaaaa
    11401 tgttttaaat gatttagctg aattttcaaa ggtttctgtt cgttctggtc aaggagttgg
    11461 aaaaacagca ttagaagcag gagcaatact ttggttctta acgtgtcgac cctacgctaa
    11521 agtaatagca acagctccga caatgaagca actttacgat gtactttggg cagaggtagc
    11581 taaatggtta aatgatagct tgatcaaaaa cttactgaag tggacaaaga ccaaaattta
    11641 tatggttggt gattcagagc gttggtttgc tacggctaga acagcgacta aaccagaaaa
    11701 tatgcaaggt tttcacgagg accatatgtt gattgtggta gatgaagctt ctggtgtgtc
    11761 tgatccaatt atggaagcta ttcttggtac gctatcaggt tttgataata agctgttgat
    11821 gtgtggaaac cctaataata ttgaaggtgt cttttacgat tcccacaatt cagaccgtga
    11881 taaatacaga gttcataaag tatcaagcta tgatagtaaa cgtacaaaca aagacaatat
    11941 agaaatgatt cttaaaaaat atggaaaaga aagtgatgtt gctcgtgtcc gtatttttgg
    12001 agaatttccc aaaggtgcgt tggattcatt tatcagtctt gaaacggttg aattggctac
    12061 agaaaaacaa attagtgatt ctttagtcaa taaaacaacg gttgctcata ttggtgttga
    12121 cgtagctcga tatggtgatg attctacgat tctctttcct agaattgcta ccagggcatt
    12181 ggagtatgag aagtattcaa aacgtagcac tatggaaaca acaggatatg tcatcaacat
    12241 ggccaagaat ctaatgagtc aatatccgag tattgataaa gtgatgatta aagtcgatga
    12301 cactggtgtc ggaggtggtg taaccgaccg cctagaagaa cttatagaag acaagcatta
    12361 tccttttgag gtgtttggag tgaataacgg ttcaacatca gaagacgatt tttacgataa
    12421 tttaggtact caactatggg gaaacatcaa ggaaatgtta gaagaaaata tgacagcaaa
    12481 tcttaacgga gaacagcctg ttattgaatt gccttctgat agttcgttaa tcaaagaatt
    12541 aagtactcgc aaattcaaaa tgacaagtag aagtcgtata cgtttagaaa gtaaagatga
    12601 tatgaaaaag cgaaatattg gtagtcccga tattgctgac gcactggctt tagcgtttta
    12661 tgagccacca agtcactatc aatttattca attttaggag gtgagttttt gattagtaca
    12721 gtattatctt tagagagata taagaagcta cgtgttaaat atgcaacaca aattgaagat
    12781 ggaatgtttg atccgaatgg ttttatagaa tatatgaaac cattttttgc tgatcgcgaa
    12841 agaaaatatc tagcttatac tagcgaaaag aatgaaatag acaatagacc aaagcctaac
    12901 actgatattg taaaagttaa taataaactt catgctggta tgtattcaat tgtcgtagac
    12961 caagcagtca atcattttac tggtatacct atcaaatggg attacgatgt atcggaacaa
    13021 aaaagaacac tcattcaaag attaaaagat aaattcttaa aaaatgatat agaacttcca
    13081 acagttccag aagcttttaa caaattaaca agcaatcttg attccatgag attcgcaatg
    13141 cttgattctg aaactgcaac ttttcaagga gcttgtggag tagctttccg attgttagag
    13201 cctgtggaag aagatgatgg ttggaagtta agagcaagta atattgaacc ttggagagca
    13261 gaaagatatg gaaatgccgg aatctatatc aaagaaaagt atgactctta ccagaaaaaa
    13321 ttttttcaag aaatgaaagt tatgacaaga aataaaattc tgacatatgc ttgctatggt
    13381 gatttgaatt ttgttacaag cggaacgttc aaaaaaattg atgagaccga taaccctttg
    13441 gggacgatta ccttggcaga atttaaaaat aatacgaatc gttattgtga ttttgaagta
    13501 gctgaggaaa ttggagatgc aattgatcgt gctttgtctg atcaacaaaa cgaaatcgaa
    13561 caatttaagc ttgcgtacat gcttgttact ggcacaacaa tgagcaaagg cactgctaaa
    13621 gaaatgatga atcagctagg gattatcaac ttaaaagacc ctactgcaaa agctgaatat
    13681 gtcacaaaaa aattagcaaa agatttcaat gaatatcata tggatctatt gaaaaaacag
    13741 ttttacacca tttgtaaagc aatcgatttt aacgatgagg tgtttaaatc aaacagttct
    13801 ggagaagctc gcaagtggca aatcattagc ctagaagcga aaacaaatac taaagagcaa
    13861 tattttagag aaggattgaa agaatgcgca gaaacaattg ctgcttttct taagtttcat
    13921 gacaaagtag aaattgagcc tgaaaaaatt atttttactt tctctagatc tttgccaact
    13981 gatttaagtt atttagctga agctttacct aaacttgcac catacgtatc caaacgaact
    14041 attcagagcc aaattccatt tgtaacagat gttgattatg aaaatgaaat gatggaattg
    14101 gaaagtggaa gtgcttatcc agatagtgaa tacaattttg gcggaggtgg caatagtgac
    14161 gatagaaacg aagtattgga ccaaacgtcg agaactggag gatcaagcaa ggctcaaaca
    14221 agaaaatcag acacttaaaa aattaactag tgtatttcct gaagcactga aagagataca
    14281 agcaaaacta ttatcacaag ctgacttaca caatatcact tatccagaaa tgatggagtt
    14341 ttacagtaca agtaatcaga aaaaatatcg tgaatatgtg gaaaaaaatt ataagtcatt
    14401 aaaaatgtat gatgcaaaat acaaagagtt tatcgatgaa ttttttccac catttgacta
    14461 tgcaaaagtc aatcgcttat tacaaatacg atcagatgta tttaaaattc ttgcagaata
    14521 tgcgatggat gcagatgtga atcaatattt ttctgatcgc ttagaggaaa ttcttcaaag
    14581 aacatattct tctaatgcta atgtttttgt tcaactttta aacgttgata taccaaatta
    14641 tttaccagaa aatgaaatta aacattattt gaactatcca tggtcaggaa agacattttc
    14701 aagaagactt tggggaaata tttcatcgct tgaacagaaa ctctctaatg ctattgtaaa
    14761 aagtgttgct agtggagaag gcgttataca cgcattaaac accatgagat tagactcaga
    14821 aatttgtgac atgtttaagt tagaagaatc aaagtataac aaagcgatag aaaatctcgt
    14881 tcgaacggag tatgcaaaat ttgcacaaga tggtattgaa aaatcatatt tagaaacagg
    14941 tattgaggaa tacaacgtat tgactgcaaa agatgagaga gtttgccgga tttgtggagg
    15001 aaaggcaagt aagaatccct ataaactgaa agatgctgtc ataggtgaaa atcgagcacc
    15061 tttccatagt cgttgtagat gtacggatgt tcctaattta ccaaaattag gaaaggatat
    15121 tgatgaagaa tatgaccgtt tatttggcga tttattagat gagtttgcac atgattcttt
    15181 tggaattaat ttgaaacgga ggaagtagaa tgaaagattt ttttgaagca gtactaacaa
    15241 ttaatgtaaa tgctgatatt gcagaagcct acaaaacagc tattgagtct gagaaccatc
    15301 ctaatggctt gagagaccat tggaatggca attatgccta cgtggttatt ggcgatcaaa
    15361 ctgttaatta tcaagataat actccagttg ataagaatac cgttaattta acgattcaat
    15421 tattatctca ttcattacca aatttaaaag aaacagttga ttggtatgaa aagatgggat
    15481 gtattgttgt tagaactgac tacaaagaag gaaagtctag taattaggct tttttatttt
    15541 gtccgaaatg acgttaaact agcgcaatac tgggcttggt tgaatggtgg ggcgcaacta
    15601 ttaaaactca aagcaatgcg gggcgtgaaa acgaatcgtg gggcgaaagg agaatgagca
    15661 tgaaacacaa atcattaatg ccaatgaatt tgcaatactt tgccgaaggt gacgatcaaa
    15721 agttttcttt tgatgacttc aaatcttttg tggaatccaa tgaagaagct caaaaattta
    15781 ttcagtcaca atctcaaagt gttgccgaca aacaattgga agcttggaaa caaaataact
    15841 tagataaaat taaacaggat accatcaagg aatatgagga atctaagaaa aacaagtcac
    15901 ctgagcaaat tcaactggaa aaactacaag ctgaatttga agcagaaaaa gcgttgcgtg
    15961 tgacaagtga taataaagca tttgttgcag aacaaattgc tggattagaa ctagatggag
    16021 agttaaaaga gtctatttct caatttatgc taaataatct tgttagttcg gatacagatt
    16081 tcactaagaa tgctgttgaa ggttttacaa gtgttttgaa tgcaattaat gagaaacatg
    16141 cagatgcatt aaaagaacta caaatgaagt ctgcatttgg tggaactcaa caatcgaata
    16201 accaggttca gcagaacaat gaaacattaa caaatccaga agaacaatta ggacaaattc
    16261 ttcaacaatt taactaggag agtgaaaaat tatgaaaaaa acatctttaa ataatttaga
    16321 gtatttggat atttcaccag cgattaatgc tatgcaagta ccaaatacac cttttttaag
    16381 ctatttactt ggtgctggaa aaacagagcc agcaaactcg acagaaatta aatggcgtga
    16441 atatgatatc aacaacgatg attcttctga aaaacttgag ggcggagaat atccagatgc
    16501 tgaatcaggt cgaacttggt ttaacaacta tactgaaatt tttagaaaat caacctctgt
    16561 atctggtaca ttagatgcta ttaatgtgaa tggagtcgga aatgaattaa ctaatcaagt
    16621 agcactacgt ggtatggaaa tgaaaattga tttgaaccga aaattgatta ctggtgtaaa
    16681 agctgatgaa aatggttcta aaggtcgtcg aatgaatggg attttgaact tgatcaattc
    16741 agcaaataag gcagaaacag ccactgcggg tgcagtaaca agaaaagata tcgatacttt
    16801 atttaaattg atgtatgaaa aaggttatat gggagaaaaa ctatgcttga tttctccaga
    16861 tatgcaggag ttaatgactg atgagttaga tggaaaatca acaaaaattg ttcagttcgg
    16921 ggaaagagta acttttggat tgcaaattgg aaatatcgtg tctaattacg gtacaggtat
    16981 tgctctacta gaaccatcat tgccaaaagg aacaattgcc gcaatcgata ctaattatgt
    17041 gaaactacgt ccattacgtg aatggagagc agaagaactt gcaaaaacaa cggattctag
    17101 acgtatcggt cttgtaggtg aatactctct tgaatacaat gcttcaaact ctggggcaat
    17161 tttaaattta aagtctgaat aaaagggagt tagtactccc ttttttgata ggaggaatta
    17221 cagtggtaaa aaaagatgaa actaaaaaag atgaagtcgt gaaatataga gtaggtaaaa
    17281 ctaaaaattt tgttgggttt gttcatccta aaactcgtag atttatcaca gcagattcaa
    17341 ataacgaatt tatcatttct atagatgata aagaagcaat tgcaattttg gaagatgcaa
    17401 ttgatgttaa tgaaatttag gaagtgatct gatggatgaa tcgctaaaaa cggaaatcat
    17461 tgagtctaca aaagaagatt ttccagattt gagtgaagaa cgcataacta atttattaga
    17521 aataattttg ctagaaattg aatcatacaa cacttgtaaa aatgatattt catgggaaaa
    17581 gttaaaaagc gtgattagtg aagtgttgta taaaataata aaaaatgaat cagaaaaaac
    17641 agtatcttca gttagacgtg gcgatacaac gattagttat gcttcaacaa caaatgatgt
    17701 tagtgagtta cttctaggct atggtgattt gatacgaaga gttattggtt gtggaggatt
    17761 ggagtttttt taatgaatga agcagatatt ttagaaacta cttatgaaga tagttgtatt
    17821 attgaaagac tgacggacat tgaagattct aatacaaata ttactattca agattacaaa
    17881 aaagtatatg ataatcctat ttcttgcgct ctttcacaag gtcaaatcga tggactagca
    17941 gtcatagaag atggagagat ggtaaatgtt tcaactgaca catataaatt atttgttcat
    18001 cctaagatta aactaaaaaa aggagatcga ataacaataa ctcaaaaggc cagtggctta
    18061 attttttctc tatttgctac taagcctttt tactatccta gtcattgtga agtgaatttg
    18121 ataggaagtg agaaaaatgg gtaatctcaa atatgaatca aatgcagaaa agatcattga
    18181 gaattttaaa aatatgactg taattgctca aaaagaagga atatccttcg ttaacgattc
    18241 aatgaataaa gtcgttagtt tagctaaacc attaactcct gtaaaatcag gtaatttaag
    18301 gcgaggatat cgtgtagtaa aagctagaaa actatcaagc ggtcgaattg ttggagcagt
    18361 tataaacaat gaacattatt ttaaatatgt agaagaaggt cataggacta aaaatggtgg
    18421 ctttgtcaaa gggaaattca tgttaactcg tgcaacgaat cttgcaaata tgtcttatat
    18481 tcctcgaaga tttaaacaaa tggcaataaa aatcgttaag aaaggaaagt aacatgaaag
    18541 atgaaatcat tgctgcaatc agtagcaaat taaaagaaat ctatcctgat gggacaatat
    18601 atcttgattc agttatgcag tcaactaaag atttttattt cgttttatca gtaatggaat
    18661 ctggaactga aaatgtagga attgatattc aaaatgtttc tttcttaatt gatattgcat
    18721 tgattgataa taaacctaat agaaatttaa ttaatgaatt agtctcacgt tgtgggactt
    18781 tttttaatac gattacaatc gatgagcaaa tattatttcc aaaagcctat ttacctgatg
    18841 aagcagatgg tgttcaacat attcgtttta cattagaatt tccacaatat attgaatgga
    18901 gtgaaagata aatgggagaa aaaagaagta aaaccggaat tatttctgtc gaaaaaccta
    18961 cttggtttcc tttggaagat gagacaggga attttccggt atatggtact gcgttgccaa
    19021 tgggaacagc agtaagtatc aaaccaacag ctaattatga aacaacacaa gattatgggg
    19081 attcagtggt tcaagatcaa tttacagctt ttggtggtgc agaagttgaa ctggaagcaa
    19141 atggatatac tcataaagtt ttatctacaa ttactggagg gaaaattgtt aaaggtggtg
    19201 ccttgcgttc tggagaagat attgcgcaag atggtgcgtt tgcatataga cgtaaaaaat
    19261 cgaatgggaa ataccgttat acggtctttt ataaaggaca gtttgctcta gattcagatg
    19321 aaacatcaac gattgaagga agtaaagtaa gttttactca tccaacttgg aagggttcat
    19381 ttgtagatgt accaggactt ggatatatgt attcagttga tgaagatgat gaaggcgtgg
    19441 ataaagcaat gatcggaaat tggtttacaa aagtagcaat tccaattgaa gaagcagaac
    19501 tttcaggagg tacagaataa tgtcaaaata tcaaacaacg attaaattaa cgaaaaaaga
    19561 tgaagaaggt aaatatgagc aagtacaatt taaatctgct gaatttctac caggcccagt
    19621 cgtagaagaa gcagcagaag taatggaggg aatgcaaaat gctgtagatg gaaaatcagt
    19681 atcagaagca ttaagtcgag cttattcatt tattgcagat attttgtttg aaggacagtt
    19741 tactggtgaa gattatcgtc aagggatcga tgctcgagaa attgcttcgt tgacaggtaa
    19801 gttattaaaa tctgttactg caggctttga tgaaacttat acagaaacga aaaaaaagta
    19861 agcgaagctc tcaaatatcc tttttttaaa cattcgatta cttatcgaga attagattta
    19921 aagacgcaat tactcgaagc aggttggacg ttaccagaaa ttgaacgtac tgactttgac
    19981 gagttaatgc gtctttttgc ttttcgagat gcagtaaaag agcatgagga tgttgagtac
    20041 tacgataact tcacccaatt ttaggaggtg atactttgaa taacgatgac ctaattctga
    20101 agatgatact agatgaatct ggttttactg ccggtatgaa taatgctgtc aaaaagttga
    20161 actcttttga tgaaacaatc gaaagaaaaa gtagaaatag tggcagttca ttaggtagta
    20221 tttggaaaat atttgctgga agttttctag ctagcggagt aactagaatc gtaggagctg
    20281 gctttgatct aatcaagggt tccataagtg gagcggttga tcgagtagat acgatgaata
    20341 acgctctacg aaatttccaa aatatgggat ttagtaattc agaaattatg aagaatatcg
    20401 gaaagaatgg gcttttatct caaggtattc aaggacttcc tactgctttg aatgatgcga
    20461 taagtcatgt tcaacttctt gcttcttcta caggtgattt aactcgttca actcaaatat
    20521 ttaaagcttt aaatgatgga attcttggtt tcggtggttc aactgatcaa gttaatgaag
    20581 ctgttattca attatctcaa agtttctcaa acggaaaagt agacgcacaa acttggaact
    20641 caatgatcaa cgctcaactt ggacctactc tttctgctat tgctaaaaag atgggaatta
    20701 caatgggaga tctgaaagaa ggtttatctc aaggtaagat ttctgttgaa gaattccaaa
    20761 atcaattaat agaaatggat accaaaggtg gcggaggact taaatcatta agtcaaatcg
    20821 ctaaagattc tactaaggga attaaaacct ctatacaaaa tgcaaaaaca gctgttacac
    20881 gtggtgttgg tgaggtaata gaaggattaa ataaagcatt agtagattca gatttaggtg
    20941 gatttaaggg aattattgat aaagtgggca gttcaatgga atcgttctta aaagtaattg
    21001 ctgcaaatat tcctacagca gtatcatttt taagtaattt atttaacgaa attcaaaaat
    21061 ttggttctgc tttgaaattc atgatgccct ttcttgttcc tgcagctacc gcttttggag
    21121 cgttcatgtt tcaacttaaa ggtataccag caataataaa aagcttcaat aattttaaga
    21181 atgccataat tggcgttgga aattcattaa agattatggg ggcaatagct gctgctaatt
    21241 catttgtttt aattattggt gcagtcgttg gtgcaatagc tgttttcggt tactttatgg
    21301 caaccaatga agagcttaga aacaaggtta tatctatttg gaatgatgtg aaagattccg
    21361 taattggtgt attaaaaaat ataaaggact ggggaattga tacttggaat tctgctaaag
    21421 aaatggcatc aaatgcagtt gagggtgtca aagatgcttg gtcaggaaca aaggaatggt
    21481 tttctaacac ctggaaaggt attaaggatg gggccacagg tttatttgat aaaacagtag
    21541 agacttcaag aaatgcagtc gatagtgtaa aaaatgcatg gtctaatatg aagaaatggt
    21601 tttctgatac ttggcaaagt ataaaagatt cagccagaga aaaatggaat ggaatcaaag
    21661 gttctattat gtcagttgct ggtccattga ttacaggtat aaagaatgca tttttacatg
    21721 ttacttttta tctagagacc ttatggaata atttagttga gattggtaaa aacgtctttg
    21781 agattttaaa aaatgttata cttgcgccgg ttttgtttat tacctccatg atttctggtg
    21841 gatgggaaga aacaaaaaat aatatgattg ctgtttggaa taatattaaa gaaagtgcca
    21901 taaatatttg ggaatctata aagaatatat ttgtaagtta ttttacaaat atttattttg
    21961 ctgcactcaa tatttggaca gggttcaagc ttacattgat aaatatttgg aatgaagtgg
    22021 taaatcaagc taaatcgatt tggttaaaca taaagtattt ttttattaat ctttggattg
    22081 atataaaata ttttgcaatt cagaaatgga ttgaattgaa gttttctatt attcaaactt
    22141 ggatcgattt aaaatataat gccattacta cttggaataa tattaaacag tttttcaaag
    22201 atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca
    22261 tgattaatac atggaataac attaaggact ctttctggaa tattgttact ggaattgtta
    22321 attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg
    22381 taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca
    22441 ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag
    22501 gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa
    22561 gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata
    22621 aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac
    22681 ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta
    22741 gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg
    22801 ttcgaaagat tactacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg
    22861 gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat
    22921 tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga
    22981 gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac
    23041 aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag
    23101 gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt
    23161 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
    23221 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
    23281 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca
    23341 gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
    23401 attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa
    23461 ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta
    23521 tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt
    23581 cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc
    23641 ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg
    23701 aaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta
    23761 gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag
    23821 agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta
    23881 tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg
    23941 actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg
    24001 acatacgact ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg
    24061 ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta
    24121 aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga
    24181 ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa
    24241 caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg
    24301 gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc
    24361 agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt
    24421 actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg
    24481 ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt
    24541 ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg
    24601 gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag
    24661 ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga
    24721 ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg
    24781 caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata
    24841 atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt
    24901 ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg
    24961 ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga
    25021 ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa
    25081 taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga
    25141 ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg
    25201 aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta
    25261 aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag
    25321 atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc
    25381 catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa
    25441 agagagcttg gagaat
//
LOCUS       DADVXN010000032        25193 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.033, whole genome shotgun sequence.
ACCESSION   DADVXN010000032 DADVXN010000000
VERSION     DADVXN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.033"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            40..1677
                     /locus_tag="KB942_001146"
     CDS             40..1677
                     /locus_tag="KB942_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851590.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1822..2562)
                     /gene="truA"
                     /locus_tag="KB942_001147"
     CDS             complement(1822..2562)
                     /gene="truA"
                     /locus_tag="KB942_001147"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6851591.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2753..3388)
                     /locus_tag="KB942_001148"
     CDS             complement(2753..3388)
                     /locus_tag="KB942_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBB6851592.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3535..4776)
                     /gene="sstT"
                     /locus_tag="KB942_001149"
     CDS             complement(3535..4776)
                     /gene="sstT"
                     /locus_tag="KB942_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBB6851593.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4974..5972
                     /locus_tag="KB942_001150"
     CDS             4974..5972
                     /locus_tag="KB942_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851594.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5985..8003
                     /locus_tag="KB942_001151"
     CDS             5985..8003
                     /locus_tag="KB942_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBB6851595.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8063..8779)
                     /locus_tag="KB942_001152"
     CDS             complement(8063..8779)
                     /locus_tag="KB942_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6851596.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9006..9548)
                     /locus_tag="KB942_001153"
     CDS             complement(9006..9548)
                     /locus_tag="KB942_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6851597.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9553..9780)
                     /locus_tag="KB942_001154"
     CDS             complement(9553..9780)
                     /locus_tag="KB942_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6851598.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9859..11739)
                     /locus_tag="KB942_001155"
     CDS             complement(9859..11739)
                     /locus_tag="KB942_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6851599.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11928..12542)
                     /locus_tag="KB942_001156"
     CDS             complement(11928..12542)
                     /locus_tag="KB942_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851600.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12684..13244
                     /locus_tag="KB942_001157"
     CDS             12684..13244
                     /locus_tag="KB942_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851601.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13549..13863)
                     /locus_tag="KB942_001158"
                     /pseudo
     CDS             complement(13549..13863)
                     /locus_tag="KB942_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14117..15752)
                     /locus_tag="KB942_001159"
                     /pseudo
     CDS             complement(14117..15752)
                     /locus_tag="KB942_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16191..17060)
                     /locus_tag="KB942_001160"
     CDS             complement(16191..17060)
                     /locus_tag="KB942_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6851602.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(17080..18201)
                     /locus_tag="KB942_001161"
     CDS             complement(17080..18201)
                     /locus_tag="KB942_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6851603.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(18203..18598)
                     /locus_tag="KB942_001162"
     CDS             complement(18203..18598)
                     /locus_tag="KB942_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6851604.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(18617..19423)
                     /locus_tag="KB942_001163"
     CDS             complement(18617..19423)
                     /locus_tag="KB942_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6851605.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(19416..20204)
                     /locus_tag="KB942_001164"
     CDS             complement(19416..20204)
                     /locus_tag="KB942_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6851606.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(20218..20694)
                     /locus_tag="KB942_001165"
     CDS             complement(20218..20694)
                     /locus_tag="KB942_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6851607.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20766..23423)
                     /locus_tag="KB942_001166"
     CDS             complement(20766..23423)
                     /locus_tag="KB942_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6851608.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(23442..23846)
                     /locus_tag="KB942_001167"
     CDS             complement(23442..23846)
                     /locus_tag="KB942_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBB6851609.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(24078..24335)
                     /locus_tag="KB942_001168"
     CDS             complement(24078..24335)
                     /locus_tag="KB942_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBB6851610.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(24460..24792)
                     /locus_tag="KB942_001169"
     CDS             complement(24460..24792)
                     /locus_tag="KB942_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851611.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         7963 a         4916 c         3796 g         8518 t
ORIGIN      
        1 aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa
       61 ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg
      121 tctaagggaa tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata
      181 ggactagata atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca
      241 atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg
      301 atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg
      361 gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt
      421 tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg
      481 ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat
      541 attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact
      601 catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt
      661 tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc
      721 ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg
      781 aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct gacacatatt
      841 ttaaataata actataacaa ctatactatt ggattattct tatttctcgg attgattggt
      901 gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta
      961 tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt
     1021 aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg
     1081 atgagtatag tgatctatta tattttttct actaaaaata attcatggaa aataatggtc
     1141 tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac
     1201 aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat
     1261 catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa
     1321 tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca
     1381 attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat
     1441 aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc
     1501 acaactgaat taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca
     1561 aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat
     1621 cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt
     1681 tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata
     1741 tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt
     1801 tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc
     1861 agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc
     1921 tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt
     1981 atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga
     2041 taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat
     2101 gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc
     2161 ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac
     2221 ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc
     2281 ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc
     2341 tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc
     2401 agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac
     2461 tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata
     2521 tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg
     2581 tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc
     2641 ttctctaaga cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa
     2701 aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt
     2761 tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt
     2821 ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc
     2881 cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt
     2941 ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac
     3001 tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac gaagattcat
     3061 gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg
     3121 ctgctgtaac tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag
     3181 gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta
     3241 caggactgga aacctctcca aatacactga tcacgctctt catttgatct aattcaggat
     3301 aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga
     3361 aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt
     3421 taattgttat tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat
     3481 ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg
     3541 ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc
     3601 gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat
     3661 tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt
     3721 agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc
     3781 tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg
     3841 tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc
     3901 accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa
     3961 tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa
     4021 gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt
     4081 aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat
     4141 caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga
     4201 ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg
     4261 tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa
     4321 agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga
     4381 ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg
     4441 cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac
     4501 aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt
     4561 cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac
     4621 cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa
     4681 tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg
     4741 ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa
     4801 caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca
     4861 atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag
     4921 atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt
     4981 tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct
     5041 tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag
     5101 tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa
     5161 cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa
     5221 tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca
     5281 cactagtttg caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt
     5341 ggcttatcct aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc
     5401 ttttttgtag tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta
     5461 aatatctact attattttta acaattggat tagttggctg ctctactcta tttaataaag
     5521 acccgtttgc attacagaat ggacagagta ttttatggat tttttattta tttttaatgg
     5581 gttatggttt gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat
     5641 ttatcagctt gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca
     5701 tcagaggaaa tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt
     5761 attatacttt ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag
     5821 gaagaggaaa ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt
     5881 tagtttctta tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat
     5941 ggctgataaa tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta
     6001 tctttttatt ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt
     6061 ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc
     6121 ataaaagtta ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg
     6181 ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc
     6241 agcgacaagc acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat
     6301 tgttgatttc caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc
     6361 tcactatttc gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt
     6421 taaaaatgct gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg
     6481 tgggcggatt gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac
     6541 aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat
     6601 taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt
     6661 ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg
     6721 gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct
     6781 attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca
     6841 accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct
     6901 tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca
     6961 aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa
     7021 cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt
     7081 tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc
     7141 tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag
     7201 tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat aaattaactt
     7261 atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct
     7321 tgaaagcttt acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa
     7381 accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg
     7441 tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata
     7501 cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt
     7561 tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag
     7621 aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta
     7681 cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga
     7741 atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat
     7801 taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta
     7861 cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag
     7921 aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg
     7981 cgacacaaaa aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg
     8041 tcacaacccc ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat
     8101 ttttcaattc attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat
     8161 tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct
     8221 ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt
     8281 gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc
     8341 actccccgtt caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat
     8401 tcgtataact tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta
     8461 attgtgcgga tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag
     8521 aggaagatga agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt
     8581 tggaagactc tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc
     8641 tctctgtgct agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt
     8701 cttttttcga ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta
     8761 ctaaaatcct ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat
     8821 caaataagca atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc
     8881 taaaaactga gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat
     8941 tttaatcttc gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata
     9001 gacttttagt catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt
     9061 ctttgtactt ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc
     9121 aagccatatt tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg
     9181 aaatcaaaat cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga
     9241 ccatgttcgt aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca
     9301 ttttcaatca ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat
     9361 tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg
     9421 atcaatttca tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca
     9481 gttggtttat gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact
     9541 atattcattg atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc
     9601 cacaactgca gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt
     9661 gctcgcatta aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc
     9721 gattttttgc atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat
     9781 cgtagttctt ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa
     9841 attgattctt atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa
     9901 acgcatcgcg ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc
     9961 catgtaaata aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat
    10021 cactacattt tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa
    10081 cgattcaaaa ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc
    10141 tgatcccata gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc
    10201 tccaataaaa gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc
    10261 gtctggtaat aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc
    10321 agtcatctga ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt
    10381 agctaattgt tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat
    10441 ttttttgtcg attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc
    10501 ttgtacttgc tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc
    10561 acaaactgta tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc
    10621 tttgactgct aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg
    10681 ccccagagga tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc
    10741 atcctctgta tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt
    10801 tttatcaaag acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac
    10861 taagatgccc cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat
    10921 gactaaagcg ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact
    10981 aatcagacca acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg
    11041 atcaatgaat ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat
    11101 tttggcaaag gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc
    11161 taaaatcgtt ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc
    11221 agtcactgcg gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat
    11281 agctccacca gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc
    11341 ttcagtcgtt ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc
    11401 cttgattgac tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa
    11461 caagaatgta acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc
    11521 aatagtaacg agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat
    11581 cataataggt atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac
    11641 aaaaccagta aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat
    11701 atgatttcga tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc
    11761 attcttacga gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt
    11821 tagtcaaaaa ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga
    11881 ctcactaaaa gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt
    11941 ttcctgtaca gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt
    12001 ttttccttta gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct
    12061 gtaaaaagga atatttcttt tcctttctct cttttttcaa attctgttag caacttggta
    12121 ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc
    12181 gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta
    12241 ctttctttag caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca
    12301 aaagaacgat gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg
    12361 tcctctgcat acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat
    12421 cttccgtaaa gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca
    12481 acagcaaaat agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc
    12541 atcatttcgc ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg
    12601 tctactatct ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat
    12661 atgtcggata aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa
    12721 aagctttggc tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta
    12781 tacgcgaact aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg
    12841 atatctatga tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc
    12901 tctcttatga tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt
    12961 acgcaaatac ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga
    13021 agaaagttaa acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga
    13081 aaacgattat cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat
    13141 ccgttctcaa tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac
    13201 tcttacagaa actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag
    13261 cttagttaag aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg
    13321 tttactgaag attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt
    13381 agtgctgctt ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc
    13441 attaataaat tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt
    13501 tatagtcata ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc
    13561 attttcaagg ggaatcagct catcatctaa cattttacgc catgctcgct tcatttcttg
    13621 tgggtattta aatggtttcg cttctggatg tttggcttta tatccttcgt aatccttact
    13681 gttgataatt gctttcatat taggaattgg atcttcacct ggcatatatt gcagattaga
    13741 agcatgtaat tttttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc
    13801 acttacttgt gtaaaatagc gacgtttaat gacaaagata tcttcattgc tatctagtgt
    13861 accttcattt ctttgtgctt aaattcaaag ataattttct caataaagat ttttgattcc
    13921 agtaagattc ctttactcct tttacaacat agcgttgtcc tttgttttga aacatcttct
    13981 tcccctcttt gccattaaaa atagcccaat tctctataaa gaagattaag cttcattatt
    14041 ataatataaa gtttaaattt ggtttattac ttactttttc ttattctcta gtaagctaat
    14101 attgaaaaaa atgtaatcag atcattattt ttatatgttg taacttcctg ataagaaaac
    14161 taattaaaag aaagaggtaa ctacataaac taattaaaaa agctaacttc atagttggag
    14221 gataaatgga agaaacttca atgattgttt ctccttgtgt tccttcaatc gacactagtc
    14281 ctattatcaa tctctctttt ttgtccattt acttttgcga ctgttcccca ataatcaaat
    14341 acaggaagcg ttattttttg tcctttagat tcatgtactg tatagactat tttcgaatcc
    14401 atcacttgat aaggcatagt atgccattcc ttgccaattc ttacttcatg attctgcaaa
    14461 tgttctttgt attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc gggaaggagt
    14521 gtcgtgtaac gaataagatt ttcttgattc attaccaagt gatctctctg agtgacaact
    14581 ttatgttcat agtttgcttt tgttgcagca gatacagcca acagtaataa aacaattgcc
    14641 gttatattaa ctacttttct ttgcttagct gatgtgctct tcatgaaaac gaacgataaa
    14701 atcaacgagc caaacagtac ttgaaagcct aagattcgcc aaggaaattg aatcatttga
    14761 atgggtgtat tttgtaataa agaccatgga aaaaatttgg tggataagag ccaagagact
    14821 accgcactaa ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa gaagagtgga
    14881 cagatcaaag aaataatacc aatgattcct accgtaaagg ttgtcaggtc attattcatc
    14941 gcaccaatca aactatccga gacatttaat gcttggtgtt gtaaggtcgt tttatctggc
    15001 tcattaatcg attgaaccat gatttgtcgg aacatcggat accaaaagta tgctgttaaa
    15061 agaatcgtca ttcctactgc ctttgacaac tccacaatca accggatgct ttcttttttt
    15121 cttgtttcac ttactacaaa gccgcctaag actagtaagc caacggctat tacactcata
    15181 aaagtggaaa gaacatgtgt ataaatgagt aagctcatac tgactgctaa tggaattgca
    15241 catggctttt tctcataaat cagtgcgtaa gcataataca taactatcgg tataaccgta
    15301 agtgcaatcg cttcagcaat agcagaacga tgataaatat caatcgtacg gtacccataa
    15361 aatgtatata aacagctaaa tataaaagcc gcatcgattc tttttaaaaa ttttagtcca
    15421 taatgaaaca tcagcatgat agtacaaaca ttcaatagcc aaacatataa gatataacta
    15481 acgattaaat tattacttat ccaataaaaa atgactgccg gaaaaaaggt caagtaggga
    15541 taaaaataat tcactccgct cccataatga ttaaacgtcg taaaattgat tggaccagaa
    15601 aaaattgaag ataacccttt gactctagca aaatggaagg caatatcccc cgatgtagaa
    15661 atagtatgaa catttctaaa ataattgatt gaataaagtc cagcaataat tgctaggaga
    15721 atggacaata ttattttttt ctttacttgc attacttaat tatatactcc tttcatggat
    15781 tgctttatct agtataactt aaattttaaa taaatcgtta tttattttta ctctcactta
    15841 aaaaagcact aaaattataa gagagggagg taatggtctg aacaccaaga attgttccaa
    15901 catcgaattg cttaactaat gagttcaaag cttaattatg tggctcttat atttaccgag
    15961 cgtggattgc gatctcttta gcgaaacaaa catctttcat aaaaaaaaca gccatatccg
    16021 ctttcttgga tatgactatt tactactact ttactatctt tcattcttct tgtctcactt
    16081 atgataaggc gctgggtatc atgagtgaat gatattgctg aatacaaata gtccgaccac
    16141 attaagttga tcggacttca taagcatttt attgactaat acttttttgc ttaagaattg
    16201 attctaacat cttttgctaa agttaagcat cctatagttc ctgcattatc tgctaactta
    16261 ggtgtgacaa tgtattcttc gatatttggc gtatcgacat agccgttaat tagttctaaa
    16321 aaacgtcttt gtactttctt tttcagatgt tcttgttgca tgacaccacc accaaaaatt
    16381 ataatatctg gtgaaagcat cagggtcgta ttacaggcac attgtgcaat ataatccgcc
    16441 tcgatttccc agaacggatc ttctatagaa agatcttgtc ccttggttcc cgtacgcatc
    16501 tcgatagcgg gtccagatgc caacccttcg agacaatcat gatgaaaagg acaagtacca
    16561 gagaaagtat ctcctgaatg tctcttaact aacgtatgcc ccatttctgg atggctaaac
    16621 ccttcaacaa aaatcccatc ttgtatagct cctccaccaa ttccagttcc aacagtgtaa
    16681 taaacaacac tggataaacc ttttccatga cctgaaacat actccccgta acaagcagca
    16741 ttcacatcgg ttgtccaggc aattggaatt tcgaaatgct tattcaacgt tccaacaaaa
    16801 tcaaaatttt tccaggcagt ttttggagta gaggtgatat gtccataggt ctttgattca
    16861 cgatggatat caatcggtcc aaatgaccct actccaatgc tttctaattg ttctttatag
    16921 tgattaaaga aatcaattac caaactcatt gtttcattcg gtgtagttgt aggaaaactc
    16981 actctttcta aaacctttaa atcatcattg ccaatggcac aaacaaattt agtcccacct
    17041 gcttctattg agccatacat ttcttactcc accgtccttt tatctaggaa ttcgttcttc
    17101 cctgtctaat agtttttcga aaaactcatg tggttccttt tggtaccaat aggcaacaga
    17161 agaaatatcg tcttgacgct cataaagctt gatgtcatca tttcctaggg tttggaaagt
    17221 aacacgtaaa ttttctttga aactaatggg gtcgggtaaa tgccagcggt acaacccatg
    17281 catcggtaaa gcatcatcgc caaaaccatg aactggattt ggattaggtt ttccagcact
    17341 aaaataatcc cgggtgtgat ctcttttaga ttgaaaagga tagcccataa atagcgtatt
    17401 aaaattttga gcatatgccc tttctccaaa gttcttttta tggaaagccc aagcgccgcc
    17461 aaaataatct tctgctcctg tagatgttat tgtagggaaa tcttcatctc catctatata
    17521 aaacttaaat tctccttccc cccaccagta tctttccaat gcgcacaaag caaagtaggt
    17581 tcctacataa tacccagtgc ctttgattcc atcaataagg gtgtaatctt ttccttttgt
    17641 cgtgatgggt tcacggttcc atttagcatg aaaatacatc gcatcatccg gtacttcagt
    17701 caatgcataa ttaaatgtat aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa
    17761 agtaatctta gctttttttc gaaatggcat ctcaaaatag caattcatcc ctgatgttgg
    17821 attcactacg attggtaagg aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa
    17881 atctcctaat ggactttcta cagaaggagt ggattcatca tcccaataaa tacgcaaaat
    17941 taaatcacgt aagacgaaac tcccagcgat tgttttgtct gtcactgtca tccaaaaatg
    18001 acgtaaaatt ccagggcctt ccatttctgc taaagtaatt gtctctccag catttatcgg
    18061 aacagaaggt gacccttttc tcccaattcc tagatgactc gctgtcattg ccgctttccc
    18121 tttttcacca tttttatttt ctggtgaaat agttctactt tcctctccct tgtggagcca
    18181 tatattattt aaacaattca ttttattcgt cctctatcct ttcttcactt ttttgattat
    18241 tcatatagat gatttgttgt tgtgcctccg caataattgc tccaatatct aaattatcat
    18301 ttcctaactg caaagcgatt gacattgcca agggaagatt cattcctgca attacgtgca
    18361 ctctgtcaga aatataggta aaaaactttt gatttacact tcctccagcc atgtcagtta
    18421 aaacaattat ttcgtctgta gtatccacta actctttaat ttgttcatct atatcatcaa
    18481 tttcttcgac atatgcacat aagctactaa tttgattatg catacctgtt aaaaactcta
    18541 gtgtctcttt aaaaccctca gctagtattg aatggctagc aatgatcaat cttctcataa
    18601 ttcaaatcct tttcctttac gctaatattc caaatgcagc cataaccatc gcaaaaacaa
    18661 taacgagtag aatcaaaatg gtcatactga tttttttctt actgagtaga taatatgctg
    18721 cagcagtaat aattactgga attaatgttg gtaaaatttt gtccaacatt ggttggattt
    18781 tcatactaac ttcaccaaaa gtgaatttaa gtggcgttga aacactgata actgaagcaa
    18841 ttaatgatcc aactactgtt agccccaata ctgaagcagc ttcagttaaa taattaatct
    18901 tttcaccgta ttcagtaaca atcttcattc cctgcttata accaaattca aagcctctca
    18961 ttcggaaaaa gaagattacc atgattaaaa tgaaccataa aaagattcct acaggattat
    19021 tttctaaagc catataagcc gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa
    19081 gagagtcccc aataccagca attggaccca tcgtaccaac tttaaagtca ttaatcgcat
    19141 cttttgcttc taatccttgc ttttcctcca ttgcaacaca agcacctaaa ataatggctg
    19201 ccagccaagg ctgtgtattg taatactgaa agtgattatt taaagcttcg atgtattcat
    19261 catcatcttt gtataatttt cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt
    19321 gctgtgtttg ataattaaag gtacaaactg acatcaacca acgatagttc gctttcctaa
    19381 gatccttttt cgttacttga tatttttcgc tcattttatt catcctcccc aataaaccca
    19441 acatcagaag ctcccgcaga agcagtgatt tgtgcatctt tttcagctct atgatacaca
    19501 ttaaatgcta aagcgaatcc tatgatcgcc gctccaataa taggtacctt caaaaaagcg
    19561 gctaagaaaa atccaacaag taaataacta atgtatcgac ctgtaggcat aaagcgtaac
    19621 aacatcgcaa taccaacagc aggcaacatt tttccggcta atgttagccc atccataaac
    19681 caattaggta aaacatccaa aacgtaatta acagcagact gtcctaaagt aacggcaata
    19741 aatactggaa ttgctgcaga taaaccaaag aaaattggcc ccaaccacaa aacactattc
    19801 attttcttaa attctcgttt attcgcatat tcctgtgatt tgcgaacaac aaaaccattt
    19861 aagattttga caattacatc tagttgcacc cctaacatac ctacaggtag tcctattgct
    19921 aatccggctt ctactccaag ctggctacca aatgccgttg caataattgc gcacgttcca
    19981 tagtccggta cagatgagcc accaaaaccg gcaactccta atgacatcaa ttgaactgta
    20041 ccgccaacta tcaaacctgt ttttatatca cccatcaaaa ttcctgtgaa taatccccaa
    20101 aaaactgcgt aatttacaaa gatcatcgga atcccataat aatcaacatg tttaataaat
    20161 gctaatatag tcagcataat aatttgaaaa atagtaaaat gcattatcct tactccttta
    20221 tcctaatttt gattgaattt cctgtgcctt atccgccgga acaaattgtg taacaatttc
    20281 tacaccagaa tccaacaatt ttttatacgt gctgatattt tcatctgatg cataggctct
    20341 agaagatagc tgtaatgcat tatctttaac aacagtaccg ccaacaatta gtcggtcaat
    20401 tctaataccg ttatctacta aatgaaggat tgtttccggc tctttcacga tgacaaatac
    20461 tttttcctga tcgtatttcc cctttttaaa attcgcaatc gctgtttctt cagtgataat
    20521 tgatacagcc attccagcag gtctagctaa acgcatactt gatttttgga tgtcgttatt
    20581 tgcgacagaa tcatcgatca ccatcactct gtttgcttta gacattggtg cccattgcgt
    20641 gacaataatc ccatgcaata aacggttatc aactctcgac ataacaatac tcataaatac
    20701 ttcctccttt atctttcatt ttcttacaag caattaccat gccaaaacaa aaagaacttg
    20761 ataaatcaag ctctttttac ataatcataa acgtatttaa cctcatgaat cgggatgtcg
    20821 acactataat aatgttcaac gacactgaaa gattgcttaa aatgtgtctg aaatttttga
    20881 aattcctcac tcgtttcatt gaaataaatt tcatcttcca aagtattttg ttttactaat
    20941 cgttcaatta agcaactaat gtgaacatat aatccgaaac acgtattatt atcaaatgaa
    21001 attcctagat cattttgtaa gatgtatata gcatctgaaa cccgttctaa taatttagtc
    21061 ggatttaata tggtcaattg ctccattaaa ttcgttagac taaaattctt cagaatatta
    21121 ttgttcaaaa ctttcagttc attatcatta aagtgtctat tcaattgatt ggagaattta
    21181 cttatattca ttcctaaaat catatcttca attgaaaaga attctacatc ttctagatgt
    21241 ggatttagtg ttccaacaat aaaaagaata ttgttctctt tacgaagctc cgataaatag
    21301 ttttcattca ctaaagagta atagtcacac gtttctatga caagatcagt atcccttgga
    21361 aagctatctt caagtatcct ttttaattta aacgaagttc ctaatccgct tgcacaagaa
    21421 cagacaatca tatccttctt aatatttgat gattcataaa tataatccac attttgattt
    21481 tcttttactt gttcaagaat ctgtttaagc ggtaaatcta ataatatctg atttgcaaca
    21541 tcaatagcca ttcttgttga aacattgttt agtagtccga attttttttg agcgtctagt
    21601 tccaaattta gataaatttt ctccagtgaa cccatatcaa caagtaaaat aatttcttca
    21661 tatccggtac actcttgtaa gtatctatta attttgtctg caatctcact acttgaagtc
    21721 attagtggca tatcaatggc gtcgtatata tatctctcaa cgatcctatt agcagtactt
    21781 gctatacagg ttgccgttag atctccatga gccaaaatca gtgctaaacg tttttgctga
    21841 aattctttac tacacattaa cttaaataaa ataaccaaaa catttttttg aatttttgtt
    21901 aaataaatat cccctatttg aataaaacga ttgactaatt gaacttctcg actacaccaa
    21961 ctattaatat cattgatgaa ggaatataat ttatcgtccg atgttaattg gatttgataa
    22021 atccaagaat tcaaaaagat agacgttatt tgaagataat aatagtcaaa ttctatttga
    22081 aaatctctct ctatttcctt aacatactgt ctaaatcgaa tctcttgata ggtgggatat
    22141 gttaaagctg aattattttg gatatcaaca ttcttttgtc taaaatatct atcatatttc
    22201 tcaaaaagct gctgcttatc agtgtctttc ttccacgttt gataaagata ctctataagt
    22261 tcgttgatat taaaaacttc gtctactttt tccaaatcat ccatttgaat atattttaat
    22321 tcattctgtc ttttcataac cttcatgccg ttgaaattca cccattgtgg taaatgatac
    22381 atctgaatat aaagttcttc caaatcatct aagaaataaa aggcattggc acaggtcaat
    22441 tgaatgatat ttttcaaatt gccaacattt tcactaaagc gatagtttaa taaaaaatct
    22501 aataattgtt gagatatcag tattttcttt gctagtcttt cttcttctga tttaaaaaat
    22561 atcttgatta actgttttct ttcataaaga gaacgactgt ctaatgttgg aagtgttagc
    22621 tttacaggta ttcttctcaa caaagttgat aatagtttat cctcaggtgg ttctgatgtt
    22681 gcaaatataa tccagcaact tgatgtgtac cagttttcat tatcacccat ccgatgaaat
    22741 ttcctcgtat ccataaactg aaaaagtttc tcctgacatt cattgctcaa tgcatgaatt
    22801 tcatctaaaa ataagactcc attattagct aattctgcta aaccctttct tccatcttcg
    22861 gctcctgtat atgcaccttt tacatgccca aaaaaattgg acaagaaaag ttctggatta
    22921 ttggcatatt cactacaatt aactgaaatg aaagatgcct tctcttctat ataattattg
    22981 gccaacaaat ccttatacac ataatttgct aaatagcttt ttccagttcc agtctcgcca
    23041 tgaagtaaaa tgggtaagcc atttggcgga tagtagattg ctgctttaat ttgagcaatt
    23101 acatttcgca gacttccttg acttccgata accctttcta ttccaaccct aaaagagcta
    23161 tcataccatt tttccgcttc ctctgaagag ctaaaaattt gttgatttgg aactttctcc
    23221 ggtaaagaaa agaagattac tggacgagta ttgatttttc caaattctcc atcttttacg
    23281 aattcattca aatattgaga gacaatatta cgactaacat ttaatttttc agatattttc
    23341 tgtgctgtat tgttccccca tttattttta atctcagagt tctctatgac ataattgaca
    23401 atgcgttctt ttaattcttc catcacagca cctcctcgac actattcgaa ttttgccgac
    23461 agtatttttc cataaatatg acttgctaaa aaggctttgt ttagatcatt caattggaac
    23521 gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggcga aatcgcatac
    23581 cccaaaatat caacgattgt tgcaatcaga tcttcttttg attcttcatc tgaaagtaat
    23641 tcacgataca acatcgtttg tttgcttact gtgtcatatt cctctaagga ttcatctgct
    23701 aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat cagccccaat
    23761 aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc cgcactaaat
    23821 aacttctgat ttaattctgc acccaaaaag atacactcct atctcttatt taaagttgct
    23881 atataccctc ttattttagc atactcagtt ttagaaaaag caaaaaaata aagaaactta
    23941 ttcttgataa caagctacgt gacgtatatc cctaacacaa aaatagccat atcctttggt
    24001 ttggatacgg ctatttgata ttactcattc aattatcaaa tcacagtagg tgtgaatctc
    24061 atcgggatcg gcaatatcta cttctgatta ggatcaaagg tgattttctc aataaaggtg
    24121 tgatacaaag ctttaacttg cttcttctca ctctggataa gaaagcaatc caaactagtc
    24181 aatatctgtt ggacatcttt gacttgaatc ggttcccctt gttgttgaaa gatccctaag
    24241 atttcattct ctcgaatttg taactgtctt cgtttttcag tcaattcatt taaacgatct
    24301 tttaacatcg gaaacaattc gggactatct tccaaagcag cttcctgttt ttgctctaat
    24361 agttagattt tgcgtaccat tttcttcttt tattttcctt cgattgctga ttttaatata
    24421 gtctaaagga aatttatatt tattgtattc tttataactt caagagaact tcgtttgagt
    24481 attactgtat ttttttaatt ccataagaaa attagttatt acctgccacc taatcagcaa
    24541 atctgaacct gagtcaatat aattaatatt attttccatc tccaacattg taagtactgt
    24601 tggtgttaat atagggactt tgtacaattg tatttttttg attttatcat ctgcacaaag
    24661 gagatttttt aaatctaatc cttcttttat ttcaaaagtt gaatttttta aatctataag
    24721 agtaaaacca attccttcat actgaatttc tgaaaatcga atttttctcc cagttgatag
    24781 atcaaggggc agaaaacctt ttattacatt gtgagtgtca ccatcattta caatgtcatt
    24841 aggaattttg attttagaat tctgtggagc acctattgga agataataat tcattataat
    24901 tttgtagtct tcatgaggaa ataaagtact tttaggattg ctaagatatt ctcgtaccga
    24961 atctaagaat ggcttatcca ttgaatcttc atccccattt aaactaaaaa acattcctac
    25021 aattgctttt gcaactctaa gtgaattcat tttctttatt gaatgtgtta aatcagactt
    25081 accattcttt aaacttgtat tgttataaag gaaattttta ttgtcttctg caaatctaat
    25141 atattcctca acatactcac taccaaaaaa actattacat ttactacata acg
//
LOCUS       DADVXN010000033        24442 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.034, whole genome shotgun sequence.
ACCESSION   DADVXN010000033 DADVXN010000000
VERSION     DADVXN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.034"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..847)
                     /locus_tag="KB942_001170"
     CDS             complement(<1..847)
                     /locus_tag="KB942_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6851612.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="KB942_001171"
     CDS             complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="KB942_001171"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBB6851613.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3348..3527)
                     /locus_tag="KB942_001172"
     CDS             complement(3348..3527)
                     /locus_tag="KB942_001172"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBB6851614.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3574..5397)
                     /gene="cadA"
                     /locus_tag="KB942_001173"
     CDS             complement(3574..5397)
                     /gene="cadA"
                     /locus_tag="KB942_001173"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6851615.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5675..7954
                     /locus_tag="KB942_001174"
     CDS             5675..7954
                     /locus_tag="KB942_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6851616.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8047..8694)
                     /locus_tag="KB942_001175"
     CDS             complement(8047..8694)
                     /locus_tag="KB942_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851617.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8873..9592)
                     /locus_tag="KB942_001176"
     CDS             complement(8873..9592)
                     /locus_tag="KB942_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6851618.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9723..10874)
                     /locus_tag="KB942_001177"
     CDS             complement(9723..10874)
                     /locus_tag="KB942_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBB6851619.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11091..11831)
                     /locus_tag="KB942_001178"
     CDS             complement(11091..11831)
                     /locus_tag="KB942_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6851620.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="KB942_001179"
     CDS             complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="KB942_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBB6851621.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="KB942_001180"
     CDS             complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="KB942_001180"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBB6851622.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12792..13442)
                     /locus_tag="KB942_001181"
     CDS             complement(12792..13442)
                     /locus_tag="KB942_001181"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBB6851623.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="KB942_001182"
     CDS             complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="KB942_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBB6851624.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="KB942_001183"
     CDS             complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="KB942_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBB6851625.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14885..15412)
                     /locus_tag="KB942_001184"
     CDS             complement(14885..15412)
                     /locus_tag="KB942_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBB6851626.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15592..16377)
                     /locus_tag="KB942_001185"
     CDS             complement(15592..16377)
                     /locus_tag="KB942_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBB6851627.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16370..17239)
                     /locus_tag="KB942_001186"
     CDS             complement(16370..17239)
                     /locus_tag="KB942_001186"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBB6851628.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17240..18619)
                     /locus_tag="KB942_001187"
     CDS             complement(17240..18619)
                     /locus_tag="KB942_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBB6851629.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="KB942_001188"
     CDS             complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="KB942_001188"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6851630.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19071..19547)
                     /gene="accB"
                     /locus_tag="KB942_001189"
     CDS             complement(19071..19547)
                     /gene="accB"
                     /locus_tag="KB942_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBB6851631.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="KB942_001190"
     CDS             complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="KB942_001190"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBB6851632.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="KB942_001191"
     CDS             complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="KB942_001191"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBB6851633.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="KB942_001192"
     CDS             complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="KB942_001192"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6851634.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22577..22801)
                     /locus_tag="KB942_001193"
     CDS             complement(22577..22801)
                     /locus_tag="KB942_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6851635.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22854..23816)
                     /locus_tag="KB942_001194"
     CDS             complement(22854..23816)
                     /locus_tag="KB942_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBB6851636.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23826..24266)
                     /locus_tag="KB942_001195"
     CDS             complement(23826..24266)
                     /locus_tag="KB942_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6851637.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6690 a         5439 c         4250 g         8063 t
ORIGIN      
        1 atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa
       61 gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca
      121 aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga
      181 gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga
      241 tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg
      301 aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga
      361 tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta
      421 ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact
      481 ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt
      541 gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac
      601 taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca
      661 gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag
      721 cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt
      781 tggccaactt tttttctgct gaagaataat cttccatcat actttcaata cgactaatta
      841 cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc
      901 ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt
      961 atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct
     1021 attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct
     1081 ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca
     1141 gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct
     1201 ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt
     1261 ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt
     1321 tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt
     1381 gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc
     1441 attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct
     1501 ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat
     1561 gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct
     1621 gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt
     1681 gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg
     1741 cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc
     1801 aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca
     1861 tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag
     1921 cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg
     1981 tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca
     2041 tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt
     2101 ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc tagactaact
     2161 tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag
     2221 aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt
     2281 ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc
     2341 ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc
     2401 caataagcac ctgccactga taataattta aatacttgta tacggccagt ggatggaaca
     2461 tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct
     2521 tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct
     2581 tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt
     2641 tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg
     2701 tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga
     2761 cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca
     2821 tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg
     2881 agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca
     2941 gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta
     3001 atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa
     3061 aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc
     3121 acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct
     3181 tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag
     3241 cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt
     3301 gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat
     3361 tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct
     3421 tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat
     3481 tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct
     3541 ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga
     3601 atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc
     3661 cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa
     3721 catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt
     3781 atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc
     3841 atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc
     3901 agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc
     3961 tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga
     4021 atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat
     4081 catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg
     4141 aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc
     4201 tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc
     4261 atgattattg actactgctt ggattccttg accggtcact tctttcagtt gactaacagg
     4321 atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc
     4381 ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg
     4441 aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt
     4501 ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact
     4561 tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga
     4621 tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc
     4681 accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa
     4741 tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc
     4801 tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat
     4861 aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc
     4921 gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac
     4981 tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt
     5041 gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg
     5101 tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact
     5161 agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac
     5221 gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat
     5281 ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact
     5341 taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt
     5401 tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc
     5461 atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac
     5521 tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat
     5581 tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta
     5641 attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac
     5701 gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca
     5761 acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg
     5821 agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga
     5881 attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt
     5941 catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac
     6001 attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg
     6061 gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac
     6121 tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg
     6181 acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat
     6241 attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca
     6301 aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag
     6361 tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg
     6421 gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc
     6481 gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt
     6541 ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt
     6601 gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat
     6661 tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa
     6721 tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca
     6781 attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg
     6841 tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca
     6901 agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg
     6961 taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat
     7021 gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact
     7081 ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt
     7141 tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact
     7201 gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct ttattgatga
     7261 aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa
     7321 tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc
     7381 attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg
     7441 ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga
     7501 aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa
     7561 attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac
     7621 agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga
     7681 gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat
     7741 tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt
     7801 agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg
     7861 tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat
     7921 ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat
     7981 gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa
     8041 ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga
     8101 aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg
     8161 acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc
     8221 aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa
     8281 tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc
     8341 gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc
     8401 catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg
     8461 aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata
     8521 acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct
     8581 tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt
     8641 gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt
     8701 ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac
     8761 cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga
     8821 gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt
     8881 ctaatgccac attatggaac acatgctgta catcttctag atcttccagt gcatcaatca
     8941 ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt
     9001 ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat
     9061 ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa
     9121 catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag
     9181 caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc
     9241 catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg
     9301 tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg
     9361 tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat
     9421 tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat
     9481 cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat
     9541 tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac
     9601 ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga
     9661 atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt
     9721 atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga
     9781 tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg
     9841 gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca
     9901 agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc
     9961 aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt
    10021 gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt
    10081 ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa
    10141 cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct
    10201 gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct
    10261 gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt
    10321 cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat
    10381 cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa
    10441 acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct
    10501 tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc
    10561 tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca
    10621 cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa
    10681 tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg
    10741 cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt
    10801 cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc
    10861 ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag
    10921 taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca
    10981 atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa
    11041 tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg
    11101 tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa
    11161 aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga
    11221 taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct
    11281 tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc
    11341 cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct
    11401 acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag
    11461 acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa
    11521 aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca
    11581 aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct
    11641 gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca
    11701 gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa
    11761 gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt
    11821 tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat
    11881 agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct
    11941 ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat
    12001 ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta
    12061 tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg
    12121 ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta
    12181 ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat
    12241 atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat
    12301 gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt
    12361 gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa
    12421 ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat
    12481 cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat
    12541 tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct
    12601 catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag
    12661 aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga
    12721 agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag
    12781 tatattcatt actatagtcc atattcatac aacccttcat tttgaatata atcaattact
    12841 ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg
    12901 gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg
    12961 tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac
    13021 cattttggca aatattctac catatcccca ccgataataa agtaataatc tgtatccgga
    13081 ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat
    13141 tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt
    13201 cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc
    13261 aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca
    13321 aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat
    13381 tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc
    13441 atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg
    13501 gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct
    13561 ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact
    13621 ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc
    13681 ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc
    13741 ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc
    13801 aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc
    13861 cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca
    13921 agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt
    13981 ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac
    14041 acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc
    14101 aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt
    14161 gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt
    14221 cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc
    14281 gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt
    14341 tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca
    14401 tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta
    14461 gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt
    14521 gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt
    14581 actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa
    14641 atatccacca cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa
    14701 aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa
    14761 cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat
    14821 cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct
    14881 tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca
    14941 taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg
    15001 gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct
    15061 gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga
    15121 tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg
    15181 ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt
    15241 tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca
    15301 acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg
    15361 taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc
    15421 ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat
    15481 atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa
    15541 aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg
    15601 gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt
    15661 caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc
    15721 gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc
    15781 agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga
    15841 cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc
    15901 cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat
    15961 cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg
    16021 ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct
    16081 attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg
    16141 agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac
    16201 tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa
    16261 taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct
    16321 tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat
    16381 ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca
    16441 tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat
    16501 cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca
    16561 agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc
    16621 cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga
    16681 cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc
    16741 cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc
    16801 tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca
    16861 ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa
    16921 gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat
    16981 ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta
    17041 gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac
    17101 cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag
    17161 gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga atatatttct
    17221 ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta
    17281 aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa
    17341 tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg
    17401 ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa
    17461 tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca
    17521 gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat
    17581 ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt
    17641 cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat
    17701 cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca
    17761 tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat
    17821 tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga
    17881 tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc
    17941 gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc
    18001 gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt
    18061 cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc
    18121 ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta
    18181 tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg actgggacat
    18241 ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac
    18301 ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt
    18361 cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca
    18421 gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt
    18481 catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt
    18541 ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta
    18601 tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct
    18661 acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc
    18721 aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct
    18781 ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca
    18841 actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct
    18901 ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt
    18961 tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg
    19021 atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc
    19081 tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac aaggatttct
    19141 gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg
    19201 cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc
    19261 gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact
    19321 gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg
    19381 actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt
    19441 ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag
    19501 gattgatcaa attgactgac taattctctg atttcattga tatccaaaat tattcctccc
    19561 agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat
    19621 aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt
    19681 gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca
    19741 aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga
    19801 cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt
    19861 cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac
    19921 cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag
    19981 ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg
    20041 caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca
    20101 tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt
    20161 ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg
    20221 ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa
    20281 ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta
    20341 cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag
    20401 aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac
    20461 caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta
    20521 aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca
    20581 ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg
    20641 ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc
    20701 cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat
    20761 agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca
    20821 atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg
    20881 ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt
    20941 gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa
    21001 tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta
    21061 ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc aacccagata
    21121 cactagagag attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct
    21181 gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat
    21241 ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg
    21301 atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt
    21361 caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg
    21421 gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca
    21481 tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct tttacatcca
    21541 taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg
    21601 cagaactggt attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc
    21661 gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg
    21721 aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc
    21781 cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc
    21841 cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt
    21901 tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc
    21961 tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat
    22021 gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac
    22081 tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc
    22141 tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc
    22201 tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata
    22261 atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc
    22321 attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa
    22381 tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc
    22441 ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt
    22501 ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc
    22561 aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg
    22621 ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga
    22681 tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga
    22741 tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca
    22801 taataatata ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac
    22861 tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc
    22921 tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt
    22981 gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt
    23041 acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca
    23101 actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca
    23161 aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac
    23221 ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct
    23281 aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca
    23341 tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca
    23401 ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac
    23461 gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc
    23521 aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct
    23581 actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc
    23641 actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc
    23701 gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc attattagta
    23761 ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa
    23821 tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca
    23881 tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc
    23941 tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg
    24001 tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg
    24061 gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg
    24121 tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat
    24181 tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac aagataatca
    24241 tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa
    24301 tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt
    24361 ttttactata tgagcttttt ttaagatttt actcatttaa aacaactgat aaatcagcat
    24421 ttataatttc ttttattaga aa
//
LOCUS       DADVXN010000034        24186 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.035, whole genome shotgun sequence.
ACCESSION   DADVXN010000034 DADVXN010000000
VERSION     DADVXN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.035"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB942_001196"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB942_001196"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBB6851638.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB942_001197"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB942_001197"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBB6851639.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB942_001198"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB942_001198"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBB6851640.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="KB942_001199"
     CDS             complement(3373..4680)
                     /locus_tag="KB942_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBB6851641.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="KB942_001200"
     CDS             complement(4683..5264)
                     /locus_tag="KB942_001200"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBB6851642.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="KB942_001201"
     CDS             complement(5836..7128)
                     /locus_tag="KB942_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6851643.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="KB942_001202"
     CDS             complement(7125..7748)
                     /locus_tag="KB942_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851644.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="KB942_001203"
     CDS             complement(7751..8587)
                     /locus_tag="KB942_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851645.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="KB942_001204"
     CDS             complement(8604..9491)
                     /locus_tag="KB942_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851646.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="KB942_001205"
     CDS             complement(9507..10790)
                     /locus_tag="KB942_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6851647.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="KB942_001206"
     CDS             complement(11473..12156)
                     /locus_tag="KB942_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB6851648.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="KB942_001207"
     CDS             complement(12174..12791)
                     /locus_tag="KB942_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6851649.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="KB942_001208"
     CDS             complement(12801..13625)
                     /locus_tag="KB942_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBB6851650.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="KB942_001209"
     CDS             complement(13618..14178)
                     /locus_tag="KB942_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851651.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="KB942_001210"
     CDS             14352..15308
                     /locus_tag="KB942_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6851652.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="KB942_001211"
     CDS             complement(15613..16569)
                     /locus_tag="KB942_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBB6851653.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="KB942_001212"
     CDS             16673..17275
                     /locus_tag="KB942_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6851654.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="KB942_001213"
     CDS             complement(17363..18862)
                     /locus_tag="KB942_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBB6851655.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="KB942_001214"
     CDS             complement(18878..19402)
                     /locus_tag="KB942_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBB6851656.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="KB942_001215"
     CDS             19700..20449
                     /locus_tag="KB942_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6851657.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="KB942_001216"
     CDS             20440..21225
                     /locus_tag="KB942_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBB6851658.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="KB942_001217"
     CDS             21194..21910
                     /locus_tag="KB942_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6851659.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="KB942_001218"
     CDS             complement(22012..23046)
                     /locus_tag="KB942_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBB6851660.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="KB942_001219"
     CDS             complement(23050..24108)
                     /locus_tag="KB942_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBB6851661.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7161 a         5216 c         4216 g         7593 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaat
//
LOCUS       DADVXN010000035        23829 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.036, whole genome shotgun sequence.
ACCESSION   DADVXN010000035 DADVXN010000000
VERSION     DADVXN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.036"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(53..787)
                     /locus_tag="KB942_001220"
     CDS             complement(53..787)
                     /locus_tag="KB942_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6851662.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="KB942_001221"
     CDS             1114..1524
                     /locus_tag="KB942_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBB6851663.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="KB942_001222"
     CDS             1517..2218
                     /locus_tag="KB942_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBB6851664.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="KB942_001223"
     CDS             2218..3804
                     /locus_tag="KB942_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851665.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="KB942_001224"
     CDS             3823..4044
                     /locus_tag="KB942_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBB6851666.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="KB942_001225"
     CDS             4041..4802
                     /locus_tag="KB942_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBB6851667.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="KB942_001226"
     CDS             5182..5691
                     /locus_tag="KB942_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6851668.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="KB942_001227"
     CDS             complement(5766..6305)
                     /locus_tag="KB942_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBB6851669.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB942_001228"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB942_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBB6851670.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="KB942_001229"
     CDS             7318..8169
                     /locus_tag="KB942_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBB6851671.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="KB942_001230"
     CDS             8197..8832
                     /locus_tag="KB942_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBB6851672.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="KB942_001231"
     CDS             8852..9625
                     /locus_tag="KB942_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6851673.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="KB942_001232"
     CDS             10033..10830
                     /locus_tag="KB942_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBB6851674.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="KB942_001233"
     CDS             10808..11221
                     /locus_tag="KB942_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6851675.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="KB942_001234"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="KB942_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6851676.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="KB942_001235"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="KB942_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBB6851677.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="KB942_001236"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="KB942_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBB6851678.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB942_001237"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB942_001237"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBB6851679.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="KB942_001238"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="KB942_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBB6851680.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="KB942_001239"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="KB942_001239"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBB6851681.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="KB942_001240"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="KB942_001240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBB6851682.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="KB942_001241"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="KB942_001241"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBB6851683.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="KB942_001242"
     CDS             21905..22483
                     /locus_tag="KB942_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851684.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="KB942_001243"
     CDS             22535..23017
                     /locus_tag="KB942_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBB6851685.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="KB942_001244"
     CDS             23134..23508
                     /locus_tag="KB942_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851686.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5115 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DADVXN010000036        23653 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.037, whole genome shotgun sequence.
ACCESSION   DADVXN010000036 DADVXN010000000
VERSION     DADVXN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.037"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(26..601)
                     /locus_tag="KB942_001245"
     CDS             complement(26..601)
                     /locus_tag="KB942_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6851687.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="KB942_001246"
     CDS             complement(606..1580)
                     /locus_tag="KB942_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HBB6851688.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="KB942_001247"
     CDS             1863..2831
                     /locus_tag="KB942_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6851689.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="KB942_001248"
     CDS             2928..3221
                     /locus_tag="KB942_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6851690.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="KB942_001249"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="KB942_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBB6851691.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="KB942_001250"
     CDS             3678..4433
                     /locus_tag="KB942_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6851692.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="KB942_001251"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="KB942_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBB6851693.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="KB942_001252"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="KB942_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBB6851694.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="KB942_001253"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="KB942_001253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBB6851695.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="KB942_001254"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="KB942_001254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBB6851696.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="KB942_001255"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="KB942_001255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBB6851697.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="KB942_001256"
     CDS             9170..9712
                     /locus_tag="KB942_001256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBB6851698.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="KB942_001257"
     CDS             9735..11291
                     /locus_tag="KB942_001257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBB6851699.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="KB942_001258"
     CDS             11305..12207
                     /locus_tag="KB942_001258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBB6851700.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="KB942_001259"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="KB942_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBB6851701.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="KB942_001260"
     CDS             13650..14072
                     /locus_tag="KB942_001260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBB6851702.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="KB942_001261"
     CDS             14541..14771
                     /locus_tag="KB942_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBB6851703.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="KB942_001262"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="KB942_001262"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6851704.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="KB942_001263"
     CDS             16182..16358
                     /locus_tag="KB942_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6851705.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="KB942_001264"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="KB942_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBB6851706.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="KB942_001265"
     CDS             16853..17320
                     /locus_tag="KB942_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6851707.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="KB942_001266"
     CDS             17405..18289
                     /locus_tag="KB942_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6851708.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="KB942_001267"
     CDS             18403..18639
                     /locus_tag="KB942_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBB6851709.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="KB942_001268"
     tRNA            18727..18807
                     /locus_tag="KB942_001268"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="KB942_001269"
     tRNA            18814..18885
                     /locus_tag="KB942_001269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="KB942_001270"
     CDS             19011..19181
                     /locus_tag="KB942_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851710.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="KB942_001271"
     CDS             19401..20108
                     /locus_tag="KB942_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6851711.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="KB942_001272"
     CDS             complement(20167..20811)
                     /locus_tag="KB942_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBB6851712.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="KB942_001273"
     CDS             20938..21225
                     /locus_tag="KB942_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851713.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="KB942_001274"
     CDS             21333..21653
                     /locus_tag="KB942_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6851714.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="KB942_001275"
     CDS             21971..23047
                     /locus_tag="KB942_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6851715.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23653
                     /locus_tag="KB942_001276"
                     /pseudo
     CDS             23044..23653
                     /locus_tag="KB942_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DADVXN010000037        23634 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.038, whole genome shotgun sequence.
ACCESSION   DADVXN010000037 DADVXN010000000
VERSION     DADVXN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.038"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="KB942_001277"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="KB942_001277"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBB6851716.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="KB942_001278"
     CDS             1011..2258
                     /locus_tag="KB942_001278"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBB6851717.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="KB942_001279"
     CDS             2334..2678
                     /locus_tag="KB942_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851718.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="KB942_001280"
     CDS             complement(2795..3121)
                     /locus_tag="KB942_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851719.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="KB942_001281"
     CDS             complement(3736..4938)
                     /locus_tag="KB942_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6851720.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="KB942_001282"
     CDS             5055..5570
                     /locus_tag="KB942_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6851721.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="KB942_001283"
     CDS             complement(5769..6215)
                     /locus_tag="KB942_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6851722.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB942_001284"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB942_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6851723.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="KB942_001285"
     CDS             complement(8094..8723)
                     /locus_tag="KB942_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6851724.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="KB942_001286"
     CDS             complement(8707..9171)
                     /locus_tag="KB942_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6851725.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="KB942_001287"
     CDS             complement(9658..10926)
                     /locus_tag="KB942_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6851726.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="KB942_001288"
     CDS             complement(11157..11894)
                     /locus_tag="KB942_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6851727.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="KB942_001289"
     CDS             complement(11961..12530)
                     /locus_tag="KB942_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6851728.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="KB942_001290"
     CDS             12658..13530
                     /locus_tag="KB942_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6851729.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="KB942_001291"
     CDS             13872..14744
                     /locus_tag="KB942_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6851730.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /locus_tag="KB942_001292"
     CDS             14741..16699
                     /locus_tag="KB942_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6851731.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="KB942_001293"
     CDS             complement(17031..17882)
                     /locus_tag="KB942_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6851732.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="KB942_001294"
     CDS             18005..18472
                     /locus_tag="KB942_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBB6851733.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="KB942_001295"
     CDS             complement(18735..19217)
                     /locus_tag="KB942_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBB6851734.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="KB942_001296"
     CDS             complement(19317..20180)
                     /locus_tag="KB942_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6851735.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20778..21653
                     /locus_tag="KB942_001297"
     CDS             20778..21653
                     /locus_tag="KB942_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851736.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21798..21950)
                     /locus_tag="KB942_001298"
                     /pseudo
     CDS             complement(21798..21950)
                     /locus_tag="KB942_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22218..23549)
                     /locus_tag="KB942_001299"
     CDS             complement(22218..23549)
                     /locus_tag="KB942_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6851737.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         7271 a         4781 c         4191 g         7391 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt ttctcacatt
    20641 ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg attatcaaat
    20701 atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa ataagtgttt
    20761 catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac
    20821 gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact
    20881 tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga
    20941 ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg
    21001 ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac
    21061 tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc
    21121 ttatagaaca tccgcaattt atcgaacgct tcaaagttaa gatgccattt catgcaataa
    21181 attattcaga taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca
    21241 aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg
    21301 aatcgctcat tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg
    21361 ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg
    21421 actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa
    21481 tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact
    21541 ccataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa
    21601 tagataaaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    21661 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    21721 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    21781 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg gtagattttc
    21841 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccactgca
    21901 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ctctaatctt
    21961 taaagataaa tattaaaata acgattaata atattaacag tctttatttt tcaccaccaa
    22021 aaacctatct ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa
    22081 aataatgatt atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa
    22141 atatgatcct tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt
    22201 tgttatctca tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca
    22261 attcgtaaaa acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca
    22321 gctgctcgcc agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa
    22381 ggaatttgca ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc
    22441 aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc
    22501 aatccaaaaa taataggttc gcttatattg aatatagcgg gagttaccga taatttagca
    22561 aagtccttat aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta
    22621 tttcccgatc cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga
    22681 atttgctctt tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga
    22741 tctaacaatg aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca
    22801 atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta
    22861 actatcgtgg taattagctt atttatatcc atattaaaca attgaatgat tataaatgac
    22921 aataaagcaa acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa
    22981 acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta
    23041 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
    23101 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
    23161 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
    23221 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
    23281 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
    23341 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
    23401 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
    23461 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
    23521 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
    23581 ttactttttc cacccaaata atacatcact tatcaaaaaa agtaaatact ttta
//
LOCUS       DADVXN010000038        23435 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.039, whole genome shotgun sequence.
ACCESSION   DADVXN010000038 DADVXN010000000
VERSION     DADVXN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.039"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(271..1767)
                     /gene="lysS"
                     /locus_tag="KB942_001300"
     CDS             complement(271..1767)
                     /gene="lysS"
                     /locus_tag="KB942_001300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBB6851738.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1878..2882)
                     /gene="dusB"
                     /locus_tag="KB942_001301"
     CDS             complement(1878..2882)
                     /gene="dusB"
                     /locus_tag="KB942_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBB6851739.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2883..3770)
                     /gene="hslO"
                     /locus_tag="KB942_001302"
     CDS             complement(2883..3770)
                     /gene="hslO"
                     /locus_tag="KB942_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBB6851740.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3987..6098)
                     /gene="ftsH"
                     /locus_tag="KB942_001303"
     CDS             complement(3987..6098)
                     /gene="ftsH"
                     /locus_tag="KB942_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBB6851741.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6188..6733)
                     /gene="hpt"
                     /locus_tag="KB942_001304"
     CDS             complement(6188..6733)
                     /gene="hpt"
                     /locus_tag="KB942_001304"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBB6851742.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6733..8178)
                     /gene="tilS"
                     /locus_tag="KB942_001305"
     CDS             complement(6733..8178)
                     /gene="tilS"
                     /locus_tag="KB942_001305"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBB6851743.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8298..8768)
                     /locus_tag="KB942_001306"
     CDS             complement(8298..8768)
                     /locus_tag="KB942_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBB6851744.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8814..9239)
                     /locus_tag="KB942_001307"
     CDS             complement(8814..9239)
                     /locus_tag="KB942_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBB6851745.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9306..9575)
                     /locus_tag="KB942_001308"
     CDS             complement(9306..9575)
                     /locus_tag="KB942_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBB6851746.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9586..11181)
                     /locus_tag="KB942_001309"
     CDS             complement(9586..11181)
                     /locus_tag="KB942_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6851747.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11196..14717)
                     /gene="mfd"
                     /locus_tag="KB942_001310"
     CDS             complement(11196..14717)
                     /gene="mfd"
                     /locus_tag="KB942_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBB6851748.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14734..15294)
                     /locus_tag="KB942_001311"
     CDS             complement(14734..15294)
                     /locus_tag="KB942_001311"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBB6851749.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15647..16621
                     /locus_tag="KB942_001312"
     CDS             15647..16621
                     /locus_tag="KB942_001312"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6851750.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16791..18191
                     /locus_tag="KB942_001313"
     CDS             16791..18191
                     /locus_tag="KB942_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBB6851751.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18269..18991)
                     /locus_tag="KB942_001314"
     CDS             complement(18269..18991)
                     /locus_tag="KB942_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6851752.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19070..19576)
                     /locus_tag="KB942_001315"
     CDS             complement(19070..19576)
                     /locus_tag="KB942_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6851753.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19699..21498
                     /locus_tag="KB942_001316"
     CDS             19699..21498
                     /locus_tag="KB942_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBB6851754.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21992..23380
                     /gene="ltrA"
                     /locus_tag="KB942_001317"
     CDS             21992..23380
                     /gene="ltrA"
                     /locus_tag="KB942_001317"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6851755.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6646 a         5056 c         4323 g         7410 t
ORIGIN      
        1 tttttgttat gtttttgaat aaatattttc ttctgtcttt tggacaagta ataatatacc
       61 aactaaattt agcttggtca acgaattttt tgtatttttt caagtttttt tcaataaacg
      121 attattcata acttttcttt ctttatcgtt cgttttcgtc ttctttaatg tctagaatag
      181 tcggcattag cttttgaact ttcgttatta tttattcctt tctacacagc aaaagctgcc
      241 taaggaattc cttagacagc ttttattcga ttatctcatt gttgggaata gcaaaacatc
      301 ccgaattgat tgtgcatcag ttagcaacat tactaatctg tcgatcccga tgcctagacc
      361 accagttggc ggcataccgt attctaaggc ttccaagaag tcttcgtcta caccgtgagc
      421 ttcgtcattt cctagttcac gttctttttc ttgcgcttcg aatctttctc tttgatcaat
      481 tggatcgttc agttctgtaa aggcattagc aaattcgcgc cccactatga atagttcgaa
      541 tcgatctgtg aatcgaggat cttctggatt tttcttcgct aaaggcgaaa cttcgactgg
      601 atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca acaaatgttt caaagaattc
      661 attgataaca tgtccgacac ccatagcttc ggttacttct acattgtgtt tttcggctaa
      721 agctaatgct tcttccaatg tcatttcttg ccagaagtct acaccagttt gttctttgat
      781 tgcatctacc atatggatgc gtttaaagtc actttctaag tcgacagctt gtccatcata
      841 tgtgattttt gctgttccta gtactttttc agctgcgtta cggataatac cttctgtcag
      901 attcattaca tcattgaaat cagtatatgc tgtgtatgct tctaacatcg taaattctgg
      961 attatgtgta gtatcaattc cttcattacg gaaaacgcgg ccgatttcat aaactttttc
     1021 cattccgcca acaattagtc gttttaaatg aagttctagc gcaatacgca aatataaatc
     1081 aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg gcagctccac ctgcctcatt
     1141 gtgcaatact ggtgtttcta cttcaatata gccattgcca tctaaatagc gacggatttc
     1201 gctgataatt tggcttcgtt tcataaatcg gtcaaagctt tcgcgattac tgattaaatc
     1261 caagtagcgt tgtctgtagc gttgttcaat attcgttaag ccatggtatt tatctggcag
     1321 tggacgaagt gcttttgaca aaatcgtgat ttctgaagct ttgatagaaa cttcgcctgt
     1381 gtctgttttc atgatttgtc cagttacacc aaagaaatct ccaaggtctg catgtttgaa
     1441 tacttcatag gcctcatcac ctacttggtc ttttcttaca taaatttgaa tttggccttc
     1501 acgatcttgt agatgcgcaa atccagcttt cccttttcct cggcgagtca tcatacgacc
     1561 agctacacta gcggtcaatc ccatttctgc tagttcttct tttgttcttg gatcaaatag
     1621 ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt tttccaaatg gatcgattcc
     1681 atcttcacgc aattgttcca ttttttgacg acgtacaagc atttgatcat ttaaatcttc
     1741 tacttgatgt tgttgttcct gtgtcacaaa agttcctcca ttctcatgtt aatctctttc
     1801 ctataatgcc aatgaaacac aaaaaaagca agccgaactt ggaatttcgg cttgaaaatt
     1861 cacgaaaaac ttagaaatca caacgtttgt ttcaatagta ctttttccgc tttttctaca
     1921 aattgatcca gcaggtcatt gatttcttgc tgtttttctg tctgattgat tgctactttc
     1981 actttcgctg ctcgaggaat cccttttaaa taataagccg catgtgttct gaattctcta
     2041 caagctactt tttctccttt gagatctgct aagcgttgta aatgaagttt agctgtagca
     2101 attttttcgg ctggtgttgg ttcaggaatc agttcccctg tttcgagata atgctgtgtg
     2161 cggtgaatca tccatggatt gcccagcgct gcacgaccaa tcatcacacc gtctgcaccg
     2221 acatagtcaa gcatccattt tgcatcttct ggcgttttga catccccgtt ccccataaat
     2281 ggaatcgtca aatgtttttt cacttctttc aatacttccc agtccgcttt tccttcatac
     2341 atctgaaccc gtgtacgtcc atgcatagcg atggcacttg ctccagctga ttgtgctgct
     2401 aatgcatttt ctacagcaaa gacgtgctct tcatcccaac caatacgcat cttgattgtt
     2461 actgggatat ttactgctga agaaacagct tggaccattt cgtatacttt ctcaggatct
     2521 aaaagccatt ttgctcctgc ttctgctttg attactttat ttactggaca gcccatattg
     2581 atatctatta tatcggcagc tgtgttttct tcgacaaatt gagcagcttc aactagacta
     2641 tctttatttc cgccaaatat ctgtaaactt aatggatgtt ctgtttcttc aatgtggagc
     2701 atctctaaag tttttttgtt tctgaaatga ataccttggt cactgatcat ttcacatacg
     2761 actaagcccg caccaaattc tttgacagtg actcgaaatg ctgaatttgt tattccagcc
     2821 atcggtgcaa caacgacacg attagggatt tccacattgc caattttcca tgtgtgttcc
     2881 acttaaatcc ctcctaaggc ttctttcttc aatccatcta attcttcttc gctatacttg
     2941 tatttgttat tacagaaaga acaaaccgct tctgcaccgt gatcttcgtc tatcattgct
     3001 tggatttctt ctgcacctaa cgcgataatt gcttctgcaa atttttcttt tgaacagtca
     3061 catttgaatt gaacaggcat tttttccaaa atgttcaatt cttctgtacc aagcaatcct
     3121 ttcaatattt cttctggtgt ctgtccttca tctaagaggg tagagattct tggtgtcgtt
     3181 tttaattgtt cttcgatctt gctgattgtt tcttcatcgg cacctggcat gatttggatc
     3241 atgaatcctc ccgccgtctt gatgctgtcg tcagtattga ccaatacact cagtccaaca
     3301 gctgaaggga tttgttctga gatagcaaga tagtaagtaa aatcttcagc gatttctcca
     3361 gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc ctaagtcttt tgtgacagta
     3421 aacattcctt gtgttccaac tgctccccgc acatcgattt taccttcact atttagagga
     3481 agactgagat gcggattttt gatgtaacct ttcacatctc cctttccatt tgcatctact
     3541 acaattcctg ttgctggacc atcgccttgg attcgaacag ttagtttgtc ttctcctttg
     3601 agcgtcgcac caagtagcag actaccgacc aatgtccgtc ccaaagcagc tgaggaggtg
     3661 tgccaagtat catgacgtct ttgtgcctcc gctacggtat ttgttgcttg aacggcgtat
     3721 gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat aatctttcat ttgttatgta
     3781 ctcctttttc cgtttttaaa ctcatagtga atcatactag caactactgc ttttttcaac
     3841 tttggaatgc ttcttttaaa aataattcag aatcatctag aaaacaggac atcaccctat
     3901 ttacaactta aataaagaaa tctcccgaaa accggttttt gttccggttt tcgggagtca
     3961 tatcagatca attgacctat atttttttat ttgtattgat cgtctgaatg attatcttct
     4021 gattgttgag aatctgaatc atgttctgct ttattttcct cattcagttc atgttttgct
     4081 tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt ctttttcttc caaagcacgt
     4141 tttgcttctt caaaagtttt cgcttctttt tcgcttggat aatcatttga ctcagtacct
     4201 tcaggcatca caccgtgttc aaacaatgac ttgattgctt tggcatccaa tgtttcgaat
     4261 tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat gagcttcgat gatttctcga
     4321 gctttatcat gagcttccat caagattttt cgtacttctt gatcaatctc gaaagcgact
     4381 tgttccgagt aagcttttgt ttgaccatag tcgcggccaa caaacacttg atggtttcct
     4441 tcatattgaa caggacctaa acgatcactc attccatatt ctgttaccat gcttcgtgct
     4501 agagctgtcg cttgctcaaa gtcattggaa gcaccagttg attggacatt gaagatgatt
     4561 tcttcagctg tacgtccacc aagtaagcct acgatttgtt caaacatatc ttctttcgtc
     4621 atcaagaact gatcttcttt tggtaaagcg atcatatatc cgccggcgcg tccgcgaggg
     4681 atgatcgtta ctttgtggac gacccgagcg cgactcaata ctaatccgac aatcgtatga
     4741 ccagcttcgt gataagcaac catttcgcgt tcttttttgc tgattacgcg atctttttta
     4801 gctggacctg caatcacgcg atcttccgct tcatcgatat cagaagcatc gattttcttt
     4861 ttatttcttc tagcagctac taacgctgct tcattcaaga cattttctaa atctgctccg
     4921 gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa cgtcatctgc catcggttta
     4981 ttccgtgcat ggactcgcaa gattgcttca cgacctttta catctggacg tccaactaaa
     5041 atctggcggt caaaacggcc tggacgtagt aacgctgggt ctagtacatc agaacggttg
     5101 gttgcagcaa tgacgattac tccttcattt ccgtcgaagc catccatttc tacaagcaat
     5161 tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca taccggcacc acgttgacga
     5221 cctaccgcat caatttcatc aatgaaaatg atagctggtg cgtttttctt cgctgtttca
     5281 aacaaatcac gaacacgact tgctccaaca ccgacaaaca tttctacgaa atcagaaccc
     5341 gagatcgagt aaaacggtac tccagcttct ccggcaacag cttttgctaa taaggtttta
     5401 cctgttcctg gaggtccttc taaaagtaca cctgccggga tacgggcacc taattcaaca
     5461 aatcggcgag gatctttcag aaattcaaca acttcaacta gttcttgttt ttcttcttcc
     5521 gcacccgcta catcagaaaa acgtacgcga ttagcttttt tgtctgcttc tttggctttt
     5581 gatttaccaa agttcatgac acggccgcca ccgccaccgc cgccgccttg ttgtcccatc
     5641 atcatgtaaa agaaaaagat gatgattacg attggcaaga agctaagcaa tagggaaacc
     5701 catataccgc tgcttgattc ttcttttact gttgtagcaa ctttgttttc ttttgccagt
     5761 tctgttactt ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa atgggtagta
     5821 gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt tagcgacggt ctgctgttct
     5881 ttgtattcac cagtgatctt atatacacca tttgcaggct gcacttcaaa ttctttgatc
     5941 ttgcctgctt gtagttgttc tgtgaatttt gagtactcga tatctgacga tcgctggccg
     6001 ttattaccaa aaatgaaata aacgaccatt accatcgcta agattacgag gacataatac
     6061 aaggcatttt tcatcatgcc gttgttcttt ttgttcatag ttgccctccc taagcgtcaa
     6121 tttaatttaa cagtttttta tcaatcctga ccattctatt gtaccataat ttaattgaga
     6181 tacagcatta atttgattga taaacactag gtttcaatac accgatgtac ggaaggttac
     6241 gatatgcttc tgcataatcc aagccataac cgaccacaaa ttcatttggt acgttgaagc
     6301 caacataatc tgcttcgata tttactactc ttccttcagg tttgtcaagt agtgtaacaa
     6361 ttttgactga ttttgctttg cggtatttga aaagatccac taaataagca agtgttcttc
     6421 cgctgtcaat gatatcttca acgatcaaca gatcgcgacc ttctacattc gtatctaagt
     6481 ctttgacgat ttttacttcc cctgacgaga ctgttgcatt cccgtagctt gaaacatcca
     6541 tgaaatccaa ttccaaatac gtatcgatag aacgaacgat atctgccatg aatggtaccg
     6601 ctccttttaa gacacctact actaggggat tggtgttttg gtaaatttct gttaactctt
     6661 tgcctagttc tttgcaacga acttgtatct cttcttgtga gatcaaaatt ctctcaatat
     6721 ccttttccaa catcaggttc tccttccaat tgcttctttt tgatatttgt aaagaagtat
     6781 gtagtgtatt ttatcagttt cttcacgaat actcaaatat gaaaaggcaa aaggtatcaa
     6841 gctatatata tttcttttgg catctgtaat gatccaagca tgctgccttt gtgaattagg
     6901 tactttctga tcgataaaat atctagatac tttttttgtg agattctctg ttaatttaat
     6961 gcggtctcct gcttttcttt tttcgacata cagttgcaca tcagcaggaa tccaaatgtc
     7021 atgagaaaat agagaccatg aatctgttgc tttgatgtcc cctgcatttt caggtttgac
     7081 cagaccaatc cattctgttt cattcaaata tattccttgt tctggaatca gcaacaagcg
     7141 ctggtcttct gtctggctag ttgcttgttt ttctttttta gagagtacaa caaaatgata
     7201 ctctcttttc agtatccaat tattgccaat actgagttgc cattggcttt ttttctgttg
     7261 ccactggtct aaaatcgact ccatttgttg ttctttcaaa ataattcctg tttttgagaa
     7321 tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca tttcttcaat
     7381 catttctgca gaaaatcgaa attgttcctc ccactgttcg acttcctttt cgatcaactg
     7441 tcccatatat ttttgaatga cttggtctgc ccattggatc tgttgcgaaa attgctggaa
     7501 atgtctcaac gcctgtgtat tttcttgttt caattgagga actaccaaat gacgaagccg
     7561 atttctctgg atatctaaca attggtttgt ctgatcctca aagtaaacaa gctgcgattc
     7621 cgctgcataa gtatataggt cttctttgct aaaactcagc aacggacgaa tcagtctgcc
     7681 cgtagcaaat ggttgtactt ctttgattcc agaaaaagta tttaactgtc ccccacgaac
     7741 gattttcatt aacaccgtct cgatttgatc atctccgtga tgagcagtca tcaacgtgtc
     7801 atattgtcct tcttccatca cgcgagtgaa ttcttgataa cgaaagttac gagcttctgt
     7861 ttctatggcg gatttggctg gatcttccca aatacttaca tataaaggga gattcttctc
     7921 ttcacaatat gagcgaagat aggcagcttc ttctgctgat tcttctctta attggtgatt
     7981 gatgtggacg atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa gtaaatggag
     8041 caaaaccatc gaatcaacac cacctgatac cgctgctaaa attctcgatc cttgttgcca
     8101 gaatccttgt tcattcccgt gccgaataaa tgactgctgt aatagcggtg cttttttccc
     8161 gcctttttca acgttcatcc tttccacacc ctttcttcct aaaaaaaaga gagcgggaaa
     8221 acgtctaaaa cgacaattaa cctgccctct gttgatacta tgacccaaat agtcgcacta
     8281 tcattatttg cggcttttta gctgcgacgt ccgccacggc caccgcgctt tccttctgtg
     8341 tttcgcttca atgaagataa gcggtcatcg ctatctttca aaaatgaact catcaatgaa
     8401 tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa aagatttttt ctgtgctgga
     8461 cgatttcggt tgtcacgggg atctttttgt tgatattttt taggacggct ttcttctcca
     8521 gattcagcag gttgttcctg cgctttccgg atagatagtc cgatttttcc atcatcattt
     8581 actgaaataa ctttaactgt tacttcatct cctacactta aaacatcatg gatgtctttt
     8641 acatagccat ttgacacttc gctaatatga actagacccg tttttccagc acctagatca
     8701 ataaacgcac caaaatttgt gattcctgat acttttcctt gcagttttgc tcctacctcg
     8761 attgacataa aaaaaatgtt cctcctaatt atttcccttt tttatattcc tgcttatttt
     8821 gcagttgtag aactagttga ttgatctgtt tcatttgtcg agctgtctgt atttgattct
     8881 aatataggat aaactttttc accgtctttt gagtaaaaga atttgcttct ggcaagttta
     8941 gctacatact catcatcttt cagcaatgaa acatcttggt gcagctgatc aacgtctgcg
     9001 ttgacttctt tcatttcagt atttgcttcg actctcaatt cgttcaattg atgcatcctt
     9061 tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc accaacaaaa
     9121 attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg aaattcagca
     9181 tactttttct ttgcatattc cgtatctagc gcagcgattt tattgctttt tttcgtcact
     9241 tggattcgct ccaatctttt tgattgtctt tttcattata ccaatatcgt gaaagcttgt
     9301 ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa taatttcgta cattttttgt
     9361 gcatcttctt ttttggttga ttcatgtaat tccgtaattt tcacttccaa cgttttgttg
     9421 ccaaactgga ctttcaaaat atcgcctact ttgacatcac tggaagattt cgccaattta
     9481 ccgttgattt gaattcttcc tttatcagct acttcttttg ctactgatcg tcttttgatg
     9541 attcgggaaa tttttaaaaa tttatctaat cgcatatctt attcctcact ttttaatttt
     9601 tttatttaat cgcaggattt tcttaccaaa aggcaacatt agccattctc gtactgtaaa
     9661 cagacgaaga cgaatcactg cataaatgaa cacagaagcc ccgatcccaa ctcctatcac
     9721 gctgacaaaa aaagcagtgg aacgatgagt tacaggacca aatactatat tcaatacacc
     9781 ataatacagc agcagtacta ctgtcataca aagaaggctg aacatcaact tccgtccaaa
     9841 atgccgttct ttccaaaaag catttagctc ttttgtttca attcgaacaa aataccaaag
     9901 cgtcacacct aatcctaaaa gggtagacag gcttgcacct gctgttccta ataaaccggt
     9961 cagaaaagaa gtagtcaatc cttttaccaa tagtccccat cctgcgcctt ttaaagaagg
    10021 gcgaaacgag ttcttgcttt gagcaatact ttgataagct tggatgactg cagtgaaagc
    10081 aatggaaaaa acaaataata caagcgctgc atttcccgca taatctttaa acaacgcata
    10141 attgatataa gggagcagca aggctaatcc caaagacgca gctaatgcta aagcggtcgt
    10201 caatctaaga tagattttgg ctgtctgtaa aaactgacga tagatccggt tggtcaaatg
    10261 acgagtcaac gcaggcagga atgtcgcact taatgcagtg gcaatcacta aacctaattg
    10321 tactaaaggc tgacctcgat catagacccc ttttgctatc ttcgcaccgt attctgacag
    10381 tccgtaaact tccaaagcat tttttacaaa gaaagagtcg atcagctgga aaaagataag
    10441 caatccacta taaatcgaaa ccagtccgcc ttctaccaga aaacgccgga tcaagggctt
    10501 tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac gctcctccat gaatcttttg
    10561 ttcatagtag tgaaggatcc catatgcgca taaaccgcca aaaacagctc cactcattgc
    10621 tgccgtccct gtttggtaca ccgtccatcc aagctttcta aaagcaaacg cactacagac
    10681 aatcacaaaa acacgaacga actgctcaac tacttgagaa acagcagttg gttccatcaa
    10741 gaatcttcct tggaaattcc ctcgataaaa agatagacca ggcatcagca aaaaggtaaa
    10801 ggaaactact tggatcagcg gtttcaattg tatgtctccc atcatccaag caatccagct
    10861 gctaaatgca aagacaaaca accacaaaaa gacacctgtc caaaatacta agggataaag
    10921 attttgcaaa gcttgctttc gctcgaaagg attctttctt tctgcaacgt actttgatat
    10981 gaactgcgga agaccggata aagctaaagt cattgctatc ccataaattg gataaacttg
    11041 ctgataaaca taaaaacctt cgtcgccgac taagttttgg aggggaattc ggtaaagggc
    11101 acttaatact tttgctatga aagaagcaat cgtcaataca aatgctcctt gcatgacgcg
    11161 ccgcatttct ttatgatcca aatcattgct ccttcctacg ctgacagata tttttgctca
    11221 cgcaacgctt tgacaaactt ttggatttcc tgaagccata ctgcttcttc catttggttg
    11281 gggatcgtca agcgtatcac catctgttct ttttcaacgc ctagactggc ttttagtttt
    11341 gtcgctgtca gtgcttcgaa taactgttcc acactatagc gttttgttcc aactttactt
    11401 agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt ctaggagcgc tcgatcgcca
    11461 tccattttga tctgcccaat tgtcaagaga tgtgctactt cttctggata ttctccaaaa
    11521 cggtcgagca gatcatcttc tagttcatct agcatttctt gcgaatcaag ttcacgaatt
    11581 cgtttataga tctcgatttt ctgccgttga tctgccacgt atgtttcagg tagatacgca
    11641 tcgacaccga ggtcgatctc aacagtggtc ttctccactt gactattctt tccttgttta
    11701 cgagaaacag cttcgcttag catttgagaa tacatatcga aaccgactgc atcaataaat
    11761 ccatgctgct gagcacctaa gagattacct gcaccgcgaa tggaaagatc ccgcatcgca
    11821 atcttgaatc cagatcccag ttctgtaaaa tctttgattg cctgaagacg tttttcactg
    11881 acttcgttaa ggatcttctg ctgctcatac ataaagtaag catatgcgac acggctgctt
    11941 cttcctaccc gccctcgcaa ttggtataac gtggataatc ccatataatc agcgttctca
    12001 acgaacaacg tatttgcgtt cgggatatct actcctgttt caatgatggt tgtagtgaca
    12061 agaacatcat attgaccttc aataaattca aaaagcgtat tttctaattg cgcctctgtc
    12121 atttgcccat gagcataacc gatccgtgct tccggaacga gttcttggat ttcttctact
    12181 ttttgttcga tcgtttctac ccgattatat aaatagaata cttgtccacc tcgtcccatt
    12241 tctcgatgga ttgcttcgcg aatagcccca ggattttttt ccataacata cgtttggatc
    12301 ggataacgat tcgcaggcgg tgtttcgatt actgaaagat cccgcacgcc taacatcgac
    12361 atatgcaaag ttctcggaat cggtgttgca gtcagagtca aaacatccac ttgtgcacgt
    12421 aactgtttca atcgttcctt atgttttaca ccaaaacgtt gttcttcgtc aatgatcagt
    12481 agacccaaat cgctaaattc aatatctttt gacaaaattc ggtgggttcc tacaacaata
    12541 tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt gttgctttct tgttctgaaa
    12601 cgacttaata gcccgatatt tacggggaaa ccttcaaatc tttccagcat cgtttcatag
    12661 tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta cttgtttgct ttcctttatc
    12721 gctttaaagg ccgctcgaag cgctacttct gttttcccat aaccaacatc acctacgagt
    12781 agtcgatcca ttggtttttc tttttccata tcccgtttga tctccgctgc acttctcaac
    12841 tggtcatcag tttcagagta ggggaaggca ttttcgaatt ctttttggta ggcgtcatca
    12901 ggtccaaaag catatccttt ttctgactct cttgcggcat ataatttgat tagatcgtca
    12961 gcgatatcct cgatttttga agagactttt cgcttggttt tcgtccattc acttccgcct
    13021 agcttattga tgcgaggcgc tttggcttca gaggcgacat atttttggat cagattcagt
    13081 tgagtaacag gaataaacag tttgtcgtcg ttttgataca aaatagtcat atagtcttgg
    13141 tgaacgccat ctacttctag cgtttccatc ccaatgtatt tcccgatacc atgattggca
    13201 tggacaacgt aatctcctgc ttttaactca ttatagcttt tcaatcgctc tgcgtttgtt
    13261 actgtttgac gtctcgtttg ttttttcgtc gttttttgga aaatttcttt ttctgtgata
    13321 gctactattt tttcttgagg aagttcaaaa ccagcctgca aagctccttc aactaattgt
    13381 acctgtcctc tgatcaatgc atttttttct gtttctacta ctaatatgtc ttcatctcga
    13441 agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa taaagatcac aacagtctgt
    13501 ttttgcttgc gccaacggtc catttctgtt ttcagcaatg gcatttgtcc gaagaactgt
    13561 tgcatcgtcc gatattgaaa attatggatt gcttggaaac gaagatttcc catccctttt
    13621 tggaagagtg agaaaaaggt tgtggtgaac ttttccttct gcacgatttt ttgtatatct
    13681 gctgaaaatg tctgctcaga aaacacccgc aattcgctga ttttttgagt ctgccattct
    13741 gccgcttccc tttcgatttc ccgatttgtt tccatgattc gctgataatc atcaacgact
    13801 agtaaactat ttgaagggaa ataatccaag atcgtcacag attcttggta aagaagatct
    13861 gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc agcttgaagt cagctgaccg
    13921 aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg tcgcagcaag ccgtttttct
    13981 agcatttttt gcatttgctc tattccatgt tctagatcct ctggtccgta gacttgttca
    14041 ctcgttgggg aaacccagat ttcttccgtt ttttcgattg agcgttgtgt ttctgcttca
    14101 aaataccgca tagaatctat ctctacatca aatagttcta cacgaacagg ataggttgtc
    14161 gttaacgggt aaatatcgat aatgcttcct cgaatactga attctcccgg ttttgcgacc
    14221 atctcttctc gtacatagcc catcaaagtc aattggcgag gaagattttc cacgtctatc
    14281 tcatcaccaa tatgccaatt caattgcgcc tgtagccaag ttttcttatt cggcagtcgt
    14341 ttgtgcaaag atgcgactgg caacaaatag atacctgctt ttttttcttg aagtgcgtta
    14401 agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa aggccatttc agcagaaagc
    14461 acttcatcca ctggaaataa atggatctga tcttgcggaa tcacatgttg cagatcttct
    14521 gctaactgat tcgcataata caaatttggc atcagaataa tcatgttttt atttgttttc
    14581 ttaaacgctc gagcaaatag aagtgttttc gctgagcccg ctaatcctgt gattagttgt
    14641 ctaggcattt tttcttctaa attgctgacc cattctttta cttgcggatc ttttccgact
    14701 aattgaataa tatccattcc ttttcctcct tcctcaattg aatcgattca tcgcatccac
    14761 gaacgaatgc ccttctatga gatattcgac tgctttaaca gattgacaca tgctttcttc
    14821 tatcaaaggc tgattttctt tgctgaaagg actaagcaca tgctggacta ctgttttttt
    14881 cccttctggc ctgccgattc ccaccttgat tcgatcaaag acattcgtat tcaaatggga
    14941 aataatactt ttgataccgt tatgcccgcc ggcacttccc ttttgacgga gccggatttt
    15001 tcccactgcc agatccaaat catcgtagat tacgactaat tcctctggat aaatcccaaa
    15061 ataagtcatt agtgggccga ctgcacgacc agattcattc atgaatgttt gtggtttgac
    15121 taaaaggatc ttttctccat tatgaaaaaa ttcggcaact tctgcttcga acggattttt
    15181 tttgaaagta gcttgatgct cgcgtgcaat tttatccacg accataaagc cgatattgtg
    15241 ttttgtatat tggtatttgg tacctgggtt tcctaacccg acgatcattt tcatttttgt
    15301 tttccaccta attctaaaaa gcagcacaaa aggctggact gctaattagc ggtccagtcc
    15361 atcgagcttt tttctttttt acacttgtca atgcatagta taacacaaaa caaggcgttt
    15421 cggctaaaag agaatcgtta ataaatgata aagaaattaa aaatttcttc aaagtttaat
    15481 gaaataattg atatataaaa atagtgaaat aacgcacatg taagctttta catataatac
    15541 aaccttgtta tctgttatat tttatagcca aatagcgtga catggggctt gagaaatgtt
    15601 agcataagca tgggaaaaaa caaatcaatg gaaggattga tactaaatga ctgcagctgc
    15661 agaaaaaaaa gatcatcaaa aagttattct tgttggagac ggcgccgtag gttctagcta
    15721 tgcctttgct ctagttactc aaaatatcgc tcaagaagta ggtattgtgg atatcaatac
    15781 tgctaaaact gaaggagacg caattgactt gtctcacgct ttggcattta cttcacctaa
    15841 aaaaatctat tctgctactt acgcagacgc acatgatgct gacttagttg ttattacagc
    15901 tggcgcacca caaaaaccag gtgaaactcg tttagactta gtaaacaaaa acctaaaaat
    15961 caaccgcgat gttgtcacac aaatcgttga atcaggattt aacggtatct ttttagttgc
    16021 cgctaaccca gtagatattt tgacttactc aacttggaaa ttctcaggtt tccctaaaga
    16081 acgcgtaatc ggttcaggta cctctcttga ctcagctcgt ttccgtcaag cacttgcgga
    16141 attagttgac gttgatgcac gtaacgtcca tgcttacatc ttaggtgaac acggagattc
    16201 agaattccca gtttggtcac atgcaaatgt tgccggcttg caaatctatg aatgggtaaa
    16261 aaacaaccca gaaatcgatg aagaagctat ggttaaccta ttctttggcg tacgtgacgc
    16321 tgcttacaca attatcgaga aaaaaggcgc tactttctat ggtatcgcag tagcattagc
    16381 acgtatcact cgtgccatct tagatgatga aaatgctgtt cttcctttat cagtattcat
    16441 gaatggcgaa tatggattga acgacatcta cattggtgca cctgctgtta tcaaccgtca
    16501 aggtatccaa aaagtaatcg aaattccttt aagtgacagc gagcaagacc gtatggctgc
    16561 ttctgctaag caattaaaag aaattctaga tgaagctttt gctaaactag acgctgaata
    16621 atctttgaac atgacggaaa acatctattc ggtgttttcc gtttttttat tttatcaccc
    16681 ttatggtttc taaatttttt cataaagtag agataacctt cgtcttttcc tctaaattat
    16741 gctatcatga gaccgttcca ccctaaactt ttacttaagg agttgtcgat atgacaagat
    16801 cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc agttcttggt gttctcttag
    16861 tgctgatcag cttttatacc tatcgcagta catactacac aaagcatttt ttgccgaata
    16921 cagaaataaa tggaataaat gtcagcaatc taacagtcga aaaagcaaat gaaaaattaa
    16981 aagaagctta ttctgataaa aaactggcta ttaaagaaga tggaaaagtc tggcaggaag
    17041 taccaaaatc cgagctcggc tacaaagatg atttcacttc ggagctttct ccgatcctaa
    17101 atgaacaaaa cggatggact tggggaatga cttatgtctc agcggcagaa aaacaagaaa
    17161 tagatcctgt cagtcaaaac caccaaaaac tagacactac cgttcagaca ttgacaacta
    17221 agctgactga tttgaacaaa gaccggacac ctactactga tgcaacgatc gaaaaagcag
    17281 gtgactcttt cgttatcaag cctgaagtca atggaaatac gatcgacgtc gatgctgcgg
    17341 tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac aattgaattg actgaattca
    17401 aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa agaacaactc gcatccatga
    17461 atgctatcgc aaatgtaaaa gctacttaca gtataaacgg tgagactttc cagatcccat
    17521 caagtgatat tatgagttgg ctgacctaca atgatggaaa agtcgattta gatacagaac
    17581 aagtacgtca atatgtaaca gatttaggca caaagtataa cacaagtacg aatgacacaa
    17641 aatttaaaag tacaaaaaga ggagaagtta cggtcccagt tggcacttac agttggacga
    17701 tccaaacaga cagtgagaca gaagcattga aaaaagcgat tctggcaggc caagatttta
    17761 ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca tccattgatc gaagatacgt
    17821 atatcgaagt tgatctggag aaccagcata tgtggtatta caaagacgga aaagtggccc
    17881 ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac acctgctgga gtcttctatg
    17941 tttggaataa agaagaagat gcaactttaa aaggaacaaa tgacgatgga acaccttacg
    18001 agagtccggt aaactactgg atgccgattg actggacagg tgttggtatc cacgattcag
    18061 actggcaacc tgaatacggt ggagacttat ggaaaacccg cggttcacac ggctgcatca
    18121 acaccccacc aagtgtaatg aaggaattat tcgggatggt cgaaaaagga acgcctgtat
    18181 tggtcttcta acaaaaataa tatataaaaa gaaagcaccc gtacgattgg tacaggtgct
    18241 ttctttttat tatctaacaa tgtgctactt acatttcagc agcagtaaag acttctgaaa
    18301 catcatcatc gtcttctagc ttatctacta gacgttccaa ttgctctttt tgttcctcat
    18361 ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc ttgcgccaaa gtgaactctt
    18421 tttccaactc atctcgtaca gcagcaaaat cttctggtgc agtataaatc tcgaatactt
    18481 caggagaagt ttgcagatct tctgcccccg cctctaatac atcctcaaac atcgtctctt
    18541 catcaacagt caacccatcg cgttcaatag cgatatatcc tttgcggtca aacaagtaac
    18601 tgactgaacc cgtttctccc agtgatccac cgtttcgagt aaaagctact cggacatttg
    18661 tcgctgtccg attacggtta tctgtcaatg catggactaa gattcctact ccacccggac
    18721 catatccttc atacgtgatt tcatcgtaat tcgtttcatc tacggcactc gtagcctttt
    18781 taatcgcccg ttccacattg tcgttaggca tatttgcagc ttttgctttg tccacaacaa
    18841 gccgcaaagc tggattcata gatggatcag gaccgcctgc tttagctgcc atataaattt
    18901 cacgagaaag tttctgaaag actttccctc gttttgcatc ttgggcattt tttcttcctt
    18961 ggatattatg ccatttacta tgtcctgaca tgatttttcc tcccttgttc ttttgagaat
    19021 acacttctat tctagtacta tttcacaaaa aaacaaagcc acagccaatc tataaacgct
    19081 tcttgaatgc cgcaaaaagc acacataata tcacagcagt cagagcaccg atcgaatcca
    19141 acatgacatc ttggaacata ggtgttcgtc caccggtcat catctgatgg aattcgtcca
    19201 aacctgcgta ccctgttgct gaaagccaag caaggatcgc tgtcagccac caaattttta
    19261 gcttggggaa aacacctaaa aacaagctgc ctcccaaaat aaaataggta ccgaaatgtg
    19321 cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc cagatggttg atactcacca
    19381 tacttccacc ataattaaaa cgtatacccg ctaatgtgtc tttaaatggc tgattaggca
    19441 gccagctttc taatcgcgaa acttgtgatt gctgtccgta agtttgagaa gaactgtaaa
    19501 ataagattat aacgactatc agtgcgatta ctaaataaat atttccgttt ttcatttgtt
    19561 tttttgaaga attcataagt cacctctgta ttttatctta gacttttttt gaagaaacaa
    19621 caatcatttt catagagact tcttccataa aaaagtgcta tacttaattt tgtattacaa
    19681 tatgaaggga gttttttcat gagttattca ttaaattgga atctagattc tattttttcc
    19741 ggaggcagtc attctgatgc tttgaaccaa cggatgaagc aattggaaga ccaaatgaac
    19801 gagtattatc accgcgtaac aaaatggtca cctagttctg acaatgcaga gcagttaaac
    19861 gctattcttc aattgcaaga aacgattacg aatggtttta cccaatgcag cagctatatt
    19921 actgcattgc tttctgccaa tgtcaatgat tcagatgcaa aggtcttatc aggcaagctt
    19981 tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat tgtcaaagaa atttgcagaa
    20041 atatctgaca atgattggaa tcagatgctt tctcaatctt cgtttgaaac aattgctttt
    20101 cgtttaaacg agattcgtcg tgatggctca caattattat cagaagctga agaaaacatt
    20161 ataaatacgc tttctttaga tggtctaaat gcttggagca gtcattatga tacgattgta
    20221 gccagcattt cgattccctt cgaacaagat ggacaagtag tcgaattatc tgctggacaa
    20281 gccttcaata agatgatggg cgatccagat ccaaaagtca gagaaacact tttcgcagca
    20341 tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg atactttgaa tcatttagat
    20401 ggttttagac tttctgacta tgaacttcat ggtgtcacag attttctaca aaaaccatta
    20461 gagtacaatc ggttgaagaa agaaacattg tctgtcatgt gggatacaat ccagaaaaac
    20521 aaacaacccc ttgttaatta cttgactaga aaagctaatc ttttcggtaa agaaaaaatg
    20581 gaatggcaag atcaagatgc acctattatt ttaggcgatt tgaaagaaaa aacctttagt
    20641 tttgacgaag cagcagcatt tattatcgaa aactttaaca aattcagtcc aaaaatggct
    20701 aaatttgccc aatctgcttt tgaaaagagc tggatcgaag cagaagatcg tcctgggaaa
    20761 cgtcctggtg gttattgtac tgagttacca gaaacaaaag aatcaaggat ctttatgacc
    20821 tacagtaatt ctgttaatga ggttgcgaca cttgctcatg aactaggaca tgctttccat
    20881 agcagcacga tgtgggattt accttcttta aatcgcgaat atgccatgaa tgtcgcagaa
    20941 acagcaagta cattcgcaga attgatcgta gccgatgcta cattaaaagt cgcaaaaaca
    21001 aaagaagaaa aaatcaattt gctagataca aaactgcaaa atgctttagc tatgttcatg
    21061 aatatccatt cccgattcat ttttgaaaat cgattctatg aagcacggca agaaggatta
    21121 gtctcagaag ataagatcac agagatgatg gttgaggcgc aaaaagaagg ctatcaagga
    21181 gctttagcta cttaccatcc atatttctgg gctgcaaaat tgcatttctt tattgatgac
    21241 gtaccgtttt ataacttccc atacacattt ggctacctct ttagtttggg catctatgct
    21301 tacgccaacc aaaagggagc aaattttgaa gaagaatata tccatttact acgcgatact
    21361 gcctcaatga cgacggaaga tcttgcgaag aaacatttga atgtcgatct tacccaacca
    21421 gatttttggc aagcaggtat cgacatggtg actgaagata tccaaacgtt catggaattg
    21481 acagaagaat ttatctgatt tctattttat cacaataaaa aaagagttag tccagaatgg
    21541 gctaactctt tttgatgtgc gccctgcatg ggtgaaaact tggtggtgaa agtccactac
    21601 aggcttggca gtaggaactg ttagcgaaag acaaggcggc aatcgtgagg ttgtggctga
    21661 aggaagtcgg acgcaaactc ctgaactgac gaacagaaac tagatacaag gctgaattag
    21721 gtcggataag tcttcatcac aagacaaagt ccaatactgc ccgaaaccta tacagtaaat
    21781 ctagcagtta catgggagga aagttttcac tcttaccggg ggagatctca tgagggtgtt
    21841 gtgggcgttg aatgaaaaca aaccagcaca acgatcatgg cgacatgatg gtgaatcatg
    21901 agaagtcagc cgaggtcata gtagtaacga cactgaccgt tacgaaggac tgaacaataa
    21961 gaatcttgaa taatttagga ggtgtgagaa gatgcgacga ccgcagaaaa caagaaaact
    22021 tggctatcat caaagagcta gtgtggaaca cgaagggtat gatgaagtgc gcagcacttc
    22081 ttgcggtgaa cagaggaaac aagatggtgt agaaaatctg tttgggagaa ttctagatcg
    22141 gaataatctc aatcaagcgt atcttcaagt agtcagaaat aaaggggcgg ctggtgcaga
    22201 tgggatgacc gtcgatcaac tgcttcctta cttgaaagag caccgcgagg aattactatc
    22261 caatttacgc caagggaact ataaacctca gcctgttttg cgagtagaaa taccgaaacc
    22321 aaacggcggt gtaagaaaac taggaattcc cacggttatt gatcgtatgc ttcaacaggc
    22381 aatcaatcaa gtgttacaac cgctatttga accagaattc tcagataata gttatgggtt
    22441 tcgcccaaat cgcagcgcac atgacgcagt gaagaaagca aaatcttatt atgaacaagg
    22501 ttaccgatat gtggtggaca ttgatatgaa agcctacttt gacaccgtaa atcacgataa
    22561 gctgatgtat ttcattgagc ggaaagtaaa ggacaaacaa gtcctaaaac tgatccgtca
    22621 atacttaatg agtggtgtaa tggaaaatgg actcatcaca acaactgaag aaggaacgcc
    22681 ccaaggaggc aatctctctc ccctgttaag taacatctac ctgaacgaat ttgatcaagt
    22741 gctagaaaat ctaggacata aatttgttcg atacgccgat gactgcaata tctatgttag
    22801 aagcaaacga gcaggacagc gtgtgatgaa gaaatccatt caatttctcg aagaacaact
    22861 aaagttgacg gtaaatcgag aaaagagtgc ggtaggaagt ccattgaaac gaaagtttct
    22921 tggattctgc attctgccaa caaagaaagg tattaaaatt cgccctcacg caaaagcaaa
    22981 acagcgtgtg aaaagcaagc tcaaacaact cactaaaaga aaccgtggaa gaagtatcca
    23041 acttatttta aaagaaatca agcaactaat gactggttgg atcaactact atggaattgg
    23101 tgagatgaaa gaattcatga gggagttaaa cggttggttg aagcgaagaa tcagacaata
    23161 tatttggaaa caatggaaga atccaagaac caaaagaaaa aatctgattc gtttagggat
    23221 cgataaagcg aaagcgtatg aatggtcaaa tacaagaaaa ggtatttggt cagtctccaa
    23281 aagtcatatt cttcatcgat cattaacaga caaagaactg gcccgccaag gatatgagga
    23341 catctccttg aagtaccagt tcgtacactc aacttattga accgccgtat acgggtccgt
    23401 acgtacggtg gtgtgagagg ggcggaaggt cagtt
//
LOCUS       DADVXN010000039        23102 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.040, whole genome shotgun sequence.
ACCESSION   DADVXN010000039 DADVXN010000000
VERSION     DADVXN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.040"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            246..800
                     /locus_tag="KB942_001318"
     CDS             246..800
                     /locus_tag="KB942_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6851756.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(973..1446)
                     /locus_tag="KB942_001319"
     CDS             complement(973..1446)
                     /locus_tag="KB942_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6851757.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1458..1994
                     /locus_tag="KB942_001320"
     CDS             1458..1994
                     /locus_tag="KB942_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6851758.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2034..2405
                     /locus_tag="KB942_001321"
     CDS             2034..2405
                     /locus_tag="KB942_001321"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6851759.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2689..3960
                     /locus_tag="KB942_001322"
     CDS             2689..3960
                     /locus_tag="KB942_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6851760.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4205..5230
                     /locus_tag="KB942_001323"
     CDS             4205..5230
                     /locus_tag="KB942_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6851761.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5524..6633
                     /locus_tag="KB942_001324"
     CDS             5524..6633
                     /locus_tag="KB942_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6851762.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6716..7612
                     /locus_tag="KB942_001325"
                     /pseudo
     CDS             6716..7612
                     /locus_tag="KB942_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7628..8452
                     /locus_tag="KB942_001326"
     CDS             7628..8452
                     /locus_tag="KB942_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851763.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8484..9794
                     /locus_tag="KB942_001327"
     CDS             8484..9794
                     /locus_tag="KB942_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBB6851764.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9942..11201
                     /locus_tag="KB942_001328"
     CDS             9942..11201
                     /locus_tag="KB942_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6851765.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11211..12083
                     /locus_tag="KB942_001329"
     CDS             11211..12083
                     /locus_tag="KB942_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851766.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12095..12925
                     /locus_tag="KB942_001330"
     CDS             12095..12925
                     /locus_tag="KB942_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851767.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12965..15148
                     /locus_tag="KB942_001331"
     CDS             12965..15148
                     /locus_tag="KB942_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6851768.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15209..16429
                     /locus_tag="KB942_001332"
     CDS             15209..16429
                     /locus_tag="KB942_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBB6851769.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16426..17886
                     /gene="gtfA"
                     /locus_tag="KB942_001333"
     CDS             16426..17886
                     /gene="gtfA"
                     /locus_tag="KB942_001333"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBB6851770.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17963..19069
                     /locus_tag="KB942_001334"
                     /pseudo
     CDS             17963..19069
                     /locus_tag="KB942_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19391..19600
                     /locus_tag="KB942_001335"
     CDS             19391..19600
                     /locus_tag="KB942_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6851771.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19593..19742
                     /locus_tag="KB942_001336"
     CDS             19593..19742
                     /locus_tag="KB942_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB6851772.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19896..20561
                     /locus_tag="KB942_001337"
     CDS             19896..20561
                     /locus_tag="KB942_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6851773.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20845..21930)
                     /gene="xerS"
                     /locus_tag="KB942_001338"
     CDS             complement(20845..21930)
                     /gene="xerS"
                     /locus_tag="KB942_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBB6851774.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22264..22497
                     /locus_tag="KB942_001339"
     CDS             22264..22497
                     /locus_tag="KB942_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBB6851775.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22494..22901
                     /locus_tag="KB942_001340"
     CDS             22494..22901
                     /locus_tag="KB942_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6851776.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7903 a         3457 c         4412 g         7330 t
ORIGIN      
        1 ctaagaactt ctacggataa ctgcccgtct aaaatttaaa taagagctta gaatcttcat
       61 ataagtaagg ttaatttttt tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat
      121 ataaaatcat aaactaaatc ccttgatttt attgactttc aaaagtttat atatataagt
      181 taatgattat ggtaaaatat atacataatc attaactcag aatattttta agaagggaaa
      241 caatagtgag ctataatgta caaccgttaa ggacaaatga agagatacaa gattttcagt
      301 tttggctaag aagaacaagt aatccggaaa gagatagttt tttatttttg tttggaatca
      361 ataatgggtt acgtatgtca gatattatag gattaaaagt tggagatata cgaggcaaaa
      421 gtaagcctat tattgttgaa cataaaacag gtaagcgaaa accgattttt atagataatt
      481 tgagagaaga aattttatta tatactgaag gtaaagaaga aaatgattgg ttatttccga
      541 gtcggcaaca aggtcgacat ataacaaggg atcgtgtcta tcagatatat gcagatatag
      601 cagaaaaatt aggacgagat gatataggaa cacatacatt aagaaaaact tttgggtatc
      661 actactacaa gaaaactaga gacattgcta ccttaatgtt tatttttaat catagctcgc
      721 aagcaattac aaaaaggtac attggtataa ctgaagatga aataggagca tcacttcgtg
      781 gatttaaact aggtgtataa tttaaaaatt tttataaaca gtaaagacaa agattaaaag
      841 ctatttttat ctagaacgat tttatttcat aaagttcgcg gaataaaagc tggggttact
      901 ggtacttggt ataggttcgt gttatataaa ctaagtaatt aaataacatt tttaggttgg
      961 aaaaggatga aattaaaaaa agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa
     1021 taagaaaaat actgataact caacgtttag cagactatct aatagggata gtctgctttt
     1081 ttcgtttttg accatacttt tgaccttctg caaacttcct tgcttttcct ctgtagaact
     1141 catgaaatct gcaaaacgaa ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta
     1201 tatatcatcc atagtggttt taatatctgt atgtccaagt cgaacataca cttctttgac
     1261 agacgcacca gattcaaaga gcagactaca atgggtatga cgaaaaccat gaagtgtgat
     1321 atgttttaag ttattctctt taatgatgat tttcaaattg tgatttacat agtctaaata
     1381 aaggggctta ttttcttctg atgaaaacaa aagttgttca ccatctttta taggtttgat
     1441 tctcattttt agatattatg cccgcacgta ttcatcacat ttctattgtg aatcgcttta
     1501 ttcgaccgac atttgacttt tatcataata tactcggttt aaaattacta atgaaaacaa
     1561 ttaatcaaga cgatcatacc atgtatcacc tgtttttttc agataatcac caccgtgtgg
     1621 gaactgaact gacttttttt gaagtacaag aagggaacaa ccagtttttt ggaaccaata
     1681 cgattgaacg aacgatacta aaagttcctt ccgaagcatc attgcatttt tgggaaatat
     1741 attttgaaac acaaggcgtt tgccattatg gaatcgaatc attttctgga cgacctatct
     1801 tacgttttga agggccagat gcaacacgat tagctttagt tcccttacgc gaatttgaag
     1861 atattgatga ttatttcccg tatgtcacag accagatccc tacagagcat gccattttag
     1921 gaattgatgc cattcaacta cgcgtacaat attccgatgc gactgaacga gagttattgt
     1981 cggtaggctg gtagcacaaa gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa
     2041 tcttagaaaa tcaagacact cagttctatc aagaagttca gattattcaa gatcgtgaga
     2101 atccagttgc cattgaaggg attggtagcg tccatcatgt tgcgtttggc gtagaaaata
     2161 aatctgattt acaacgcatc gacaaacaat tacaagagcg caattttata aattcaggaa
     2221 taaaagatcg tgaatttttt gtatcacttt attatcgtga cccaaatcaa ttattgattg
     2281 aaattgctac aggggaagga aacctagatg caaaagcgta tgaaaatcaa agtccacgct
     2341 ttgaagaaat tcctttattt ttaccgcagt acttaaattt cattcgtgaa caagtggagc
     2401 aataattagc taatcaaatc catgacgaag agtaagaggt tgactcataa gtcaaaaaat
     2461 aaagaaaaac gcatcaaatc cattctaaag aatgttgatt tgatgcggtt cctattttta
     2521 taaactatca ttttcgacta ataaaactac ttatgagtca gcctctcttt ttttcttgat
     2581 ttttatacct ctttctaggt aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac
     2641 attctcaact atttcaaaat aaaggggagt accaaatgaa cgagtatatt gaaattatcc
     2701 acgcaagaga aacaacttaa aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt
     2761 tttaccagcg tatctggttc aggaaaatca tcgattgttt ttgatactat tacccaagaa
     2821 gctggtcgtc aactgaatga cacctatgat agttttaccc gtttattttt acctaaatac
     2881 agtcgtccag aggttgatga gattgataat ctttctacgg cgattgtggt caatcaaaag
     2941 aagattggtg gcagtgcgcg ctcaacgtta gggacgatta ctaacatcaa tgcgttattt
     3001 cgtgtgttgt tttcatgttt tgcaacacct agtttaggat atgcaaatgc ttattcattt
     3061 aatgatccta gtggaatgtg ttctgtctgt cagggaatcg gtaagacgat tgcattttta
     3121 ataggattgc tttatttttc ggctattgat aatgctgatt ttgtattttc tgatccattt
     3181 accgcagaag ttttgtctgg aattgaggaa caggcaaaga atcatggtta ttttgtattg
     3241 gtgcataatg ttaattcagc agaagatgtt aaaactattc agagaaattg gcgttttgag
     3301 gggttcattg ttgttggtgt acgtgtaaaa gaatttttgg aattaaataa cgtttttgat
     3361 ggtcctgttg tttatattga tacgcatctt tcaaaagaga cattaagaga acttgaacaa
     3421 caaatgaatc gtttatttgt aaatacaaat gattaccaag ctagtacatt ggcagtggaa
     3481 catttgattc attatggtca tgagaaaata gcgtttcttt cttttgacta cattgtaggt
     3541 gaaccaagtg ttattcaaga acgacatcaa gcttatatcg atacattaaa gaaatatcat
     3601 ttaaccttta aatcgcattg gatatataac aactctgaat ttgagcgaat ttatcaagaa
     3661 ttagctgaat ttactgcaat tgttgttact ggagattatt tagcggctaa attagtaaaa
     3721 tttttaaaaa ataagaataa acgagtgatg gaacaattat cgattattgg atttgatgat
     3781 attacttttg cagaattaat ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac
     3841 aagggggcga ttgcttttca ccaactgatt ggactggcca aacaagaaga aataaaaaat
     3901 caagtactac tattagaggg agaattgatt tctcgagact ctgttcacaa aatcaaataa
     3961 aaaagctctg tgaaataagt atagttaaca ataaaatatc cgaacgatag agagaatgaa
     4021 ctgtgatcta gcagttcctc gtcgcaaatt cactagaatt agcattattt actctaaatt
     4081 gttcggatat tttttcttgt ttttaattat gattacagtg cctattttta acgaaatttg
     4141 cttattttca aaatgtatga ttatatgtta gtaaacactt tcaaatatga aaagaggagc
     4201 tattatggta ggaattaggg atgttgcaaa acgggcgggt gtgtccattg caacagtatc
     4261 gcgcgtgcta aatgaggatc cgacactttc agtaaccgat gaaaccaaac gaaagattgc
     4321 agaatcggtt aatttttatc attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa
     4381 tattgcatta ataacgacag tttctgaagt agatgagttg gaagatccct attttcgaac
     4441 gattcgcaga gggattcaag tcgaagcaga acaaagcaaa gtcaatttga agaaaattat
     4501 tcgtttgtca gaaacaccgt tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat
     4561 tattgggcag gtgctatctt ctgtgattga agaagtatca gcaattaatt cgaatgtcgt
     4621 agtgattgat gaccctagta ttgaagctga agtagatgca gtttatactg atttaacaaa
     4681 agcaacgaat cagcatttag atcgattgta taaaaaagga caccgaaaca ttgcgtttat
     4741 cggaggagga cgtgttcgtt tagatagcca aggaaataaa cacgtgtcag atgatgatta
     4801 tcgaaaaata gcctatgaga cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat
     4861 ttatttagaa ggctggacca cacttgacgg aatgcaggca gcagatttac ttttgaaaaa
     4921 acatgctaaa gataagttgc cgactgctat catggtagga aatgatccta ttgctgtggg
     4981 ggtttatcgt tcacttcaaa aaaatggtct acatattcca gaagatattt cgattgtgag
     5041 ttttgataat attgaagttg ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa
     5101 tacagaagaa attggaaaac tagcagttcg catggttaat gaacgaataa aagaagttag
     5161 aaatgtttcg atcaaagtga ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa
     5221 tatcgattaa aaaagtaaac atccaaaaaa gggtgtttac ttttttgttt actaatattt
     5281 ttagtaaaaa atctcgacaa aacattaagt aaacagttat ggtgttgtta acaaaaagaa
     5341 aacgcattca aaacaggagg aattaagatg aaacatttca aaaaaatagc tttaacaggg
     5401 atagctgttt taactgggac attactcgca gcatgtggtg ggggagatag taattctaaa
     5461 gacgggcaag taactttaag ctatgcgagt tgagatactg accaagctcc aggactaaga
     5521 aaaatgttag atgaatttga aaaacaaaat cctgatatca aagtagaaat ggagacaacg
     5581 ccttgggatc aatattgggt taaattagaa gctgcaacaa cgggaggtaa tatgccagat
     5641 gtagtaacca tgcactctag cgaatcctat aaatatatgt cacagaatgc tttgatggga
     5701 cttgatgatg ttatcaaaga aaatgatatt gacatgggaa actttactga aggaattgct
     5761 gatttctata cgcttgaaga cgaactatac gcaatcccta aagatgcttc tgtagtgggg
     5821 ctatggtaca acaaagaatt atttgataat gcaggtgtcg attatccaga tgaaacatgg
     5881 acatgggaaa cactcagaca agctgcaatt gatttaacag atgaatcaaa aggcatctat
     5941 ggttttgcat cacatattgg aacagagact ggctactggc catttattta tcaaaatggg
     6001 ggagaagtat ttgccgatga caatacaaaa tcaggtttga ataccccaga agcacaagaa
     6061 gctttagctt tttatcgaga tattgcactt gttgatcatg cttcaccaac aattacagaa
     6121 ttacaagatc ctgataagtt aagtcgtttt caatcaggta gattagcaat gatgattgaa
     6181 ggaaactggt ttgcaccggg atttttgaaa aatgactatc tagttgaaaa tgttgcttct
     6241 gccgtgttac ctaagggaaa agttaatgca acaattacaa atggacttgg ttgggctgcc
     6301 agtgcgagta caaaataccc agaagaagtg aaaaaactaa tggcattttt agctagtgag
     6361 gacgccaata aaatccaagc ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa
     6421 gacgagtggg ctgcttcttc agatacaatt gatttgagtg cttttggaaa aatgttggag
     6481 tatggagtcc ctcgtccata caataaagat ggattacgag ctgaacaagc agaagttcaa
     6541 attattaacc gtattctgtc tggtgaagta actgttgaag atggaaccgc tcaaattgaa
     6601 aaagaagtca atgcgatatt gagcggaaat taaattagtt aattatacaa tatacaggcg
     6661 aaaggattct atttcgtctg tatattaatg gatgaatacc aaaaaggggg atattatgaa
     6721 aaatcgaatt tttggaaaat atcgtaaaga tcaagtgata tggggctata tttttatttt
     6781 tccaacggca ttaggattaa ttttattaaa tatatggcca gcaattcaaa cagcaatttt
     6841 aagttttcaa aaaacggtag ggttcggtga tactgaatgg gtcggattaa gaaattacca
     6901 aaaaatgttt gcagacggtg aagtttttca gtctttatta aacacattaa tttatgcagt
     6961 ggtttcgata cctagtattg ttgtattatc cttgttggtg gcagttttaa tgaatagaaa
     7021 agttaaagga ttgtcgattt atcggacaat ttattttcta ccagtcgttg ctgcaccatc
     7081 cgcagtcgca atgatttggc gctggatgtt caataatgat tatggactga ttaataacat
     7141 gttggctaaa ataggtctcg atggaccagc atggttaacg gatccatcga ttgcgattta
     7201 ttctattatc attgtaggag tttggagtgc tattggttat aacatggtgt tattactagc
     7261 cggcttataa gaaattccta aagattatta tgaagcagcg gaaattgatg gtgctagtcc
     7321 aatccagcag tttttcaaca ttacgttacc attagttaca ccaacattat tttttgtagt
     7381 tgtaacaaca gttatcaatg ctttccaagt atttgatgtt atttttatga tgattactcc
     7441 taatagtacc gccatgttta aatcacaatc attagcctat ttattctata aacattcatt
     7501 tattttaaat gacaagggat atggttcggc aatcgttatg ttccttttag tgattatatt
     7561 aattattact gcaattcaaa tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta
     7621 tcaaattatg aaaaataaat ttttttcaaa tgcgccaatc catatcatgc ttattttagg
     7681 tggtattgca atggcattac cttttatttg gatgatttta acttcaggga aaacgttggt
     7741 tgaatctacc caaattccac ctacaatttt ccctgagaaa tttcagctaa gtaactatca
     7801 acaagtactt gatttattac cattcatggc tttttatatc aatacatttt taatggttgt
     7861 tggacgagtg attggctcgg ttttattttc agcaatggct gcatatgctt gtgcgcgtct
     7921 aagttttcca gggaaaaata tccttttcag cttagtactt attcaaatga tgattcccgg
     7981 ttccattttt attactccac agtatttatt agcacaaaaa ttgggtattt taaatacaat
     8041 tccagcatta atattaccag gggtagttag tgcgtttggt acgtttttat taagacaatt
     8101 tttcttaggt ttacctgatg aattggagga agcggcctat ttagaaggtg ccaatatttg
     8161 gacgatcttt ttcaaaatta tgttgccgct tgctaaatca ggattggttg cacttggaat
     8221 attcacagcg ttgtttgcat ttaaagattt aatgtggcca ttgattgtca atacttcaat
     8281 tgataagatg cctttagctc cgggattagc aaacttacaa ggacaatatg ccacgaactt
     8341 cccacaatta atggcaggtt cgttattagc aatttggcca atagttgtga tattcatcgt
     8401 tttccaaaga aaattcattg aaggaattgc tacaacaggt agtaaattat aaaactatta
     8461 attagtgatt ggagagtaag tacatgacaa aaattacatt tattggtgca ggaagtacta
     8521 tttttgcaaa aaacgtatta ggcgatgcga tgttaactcc aagtctacaa gatgcacata
     8581 ttgcattgta cgatattgat gaaaaacgac taaaagaatc aaatttaatg cttaaaaata
     8641 ttaataaaaa ttcgaatgaa aatcgtgctg atattgtttc ttattatgat cgcaaagaag
     8701 cgttaagaga ggctgatttt gttatcaatg ctattcaagt gggtggttat aaaccttcaa
     8761 cagtgattga ttttgatatt cctaaaaaat atgggttgcg acaaactatt ggtgacacca
     8821 tggggattgg tgggattttt agaaccctac ggacactacc agtaatgtta gactttgcta
     8881 aagaaatgga agaagtttgt ccaaatgctt ggttattgaa ctatacaaat ccgatggcga
     8941 tgttaaccat gggtgtttta aaagcgacga gtattaagac tgttggttta tgtcatagcg
     9001 ttcaagtatg cgtaccagaa ttgtttgagc atttaggaat agccgatcaa tatgacttaa
     9061 atgactttca atggaaaatt gcaggcatta atcatatggc attcttgttg gaaattacaa
     9121 aaaatgggga agatttctat ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac
     9181 atccagattc tgtacgtttt gaattgttga aacgttttgg ttactatatt acagaatcaa
     9241 gtgaacacaa tgcagaatac catccatact ttattaaatc aaaataccca gaattgattg
     9301 acgaattagg gattccgttg gatgaatact tacgtcgttg tgaacaacaa attaatgatt
     9361 gggaaagcat gcgagaggac attgtaaata atggggagtt aacacatacg agaagccgtg
     9421 aatatgcttc atatatcatg gattctatta cgacaggaac tccgtcagtg attgcaggaa
     9481 atgtcttaaa taaaggctta atcacaaact taccgacaga tgtttgtgtg gaagtacctt
     9541 gtttagtcga taaaaatggc gtgcaaccaa cctatgtcgg tgaattgcca acacaattag
     9601 ctgctttaaa tcggacgaat atcaatgttc aagaactgac agtagaagcc gctttaacac
     9661 tagataaatc aaaaatttat caagcagcgt atttagatcc acatctatca tctgaattgt
     9721 ctttaacaga tatcaaaaat ttagtggatg atttaatcga agcacatggg gactatttac
     9781 ctgaatataa ttaaaaagaa gctgaaagag taaggaattt tcaattcctt actcttttct
     9841 tagttaaatt tagtaaaaat attgacaaaa cacttagtaa acagttacat tagtgttaac
     9901 aaaaagaaaa cgcattcaaa ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa
     9961 ttattttagc ttgtatagca ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc
    10021 aggaagatgc aagcggtaag gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc
    10081 aaacgattca ggagattgct aaagattttg aagcaaaaaa ccctgatatt catgtaaaag
    10141 ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac
    10201 cagatgtcat taatctttat cctcaaagta tagaattgaa agaatgggcg aaagctggtt
    10261 atttagagga cttaaccgaa gaaccgtatt tagagaatat caaaaatgga tatgcacaac
    10321 gttttgctat tgaggatcgt gtatatagca taccattaac cgctaatgtc tatggttttt
    10381 actataacaa aactgctttt gaggaaatgg ggattcaagc accagaaact tgggcagaat
    10441 ttgaaaaaat agtagctgag attaaagatc aaaacaaagt accgtttgcg attgcaggag
    10501 cagaaggctg gactttaaat ggttatcacc aactagctat agcgactgct gctggtggtg
    10561 aagaggaagc gaacaatgtt tggcgtttct cagaagttaa tggaattaat gctgaaagca
    10621 aagaaatgca aaaagatttt aatcgattag atttgttaag agaaccaaaa gctttgcaaa
    10681 acaactggca aggtgccggt tataacgata cagtagttac ttttacaaaa ggtgatgcgt
    10741 tgattatgcc gaatggttca tgggcaatgc cgatgattca ttcacaaaat ccagattttg
    10801 aagtaggaac ttttcctttt ccagcagatg aagcaggtca aagtctaacg attggcgcag
    10861 gagacctagc cttatcagtt tctgcaactt ctcaacataa agaagaagcc aaacgttttg
    10921 tagaatatat gacaacacct gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt
    10981 gtgctgttga gggcgttatc gagaatactc aggattcacc attaagtggt ttaactgaac
    11041 ttgcttttac tgatcgtcat ttagtttggt tagccaaaga ttggaatagt gaaaatgatt
    11101 tttatacctt aacaacaaat tatttacaca atggcaatca aaaagccatg attaacgcat
    11161 taaatgcttt ctttaatcca atgaaagctg acggaaggta ggaaaataaa atgaaaataa
    11221 aagaaatttt aaatcgttac tgggcaatca tttttgtagc aattccaatt attttgcaga
    11281 tgattttttt ctactttcca atggttcaag gtgcttttta cagtttaact aattggactg
    11341 gtctgactta taattttgat tttgtgggtt tgaacaatta taagattctt ttttctgatc
    11401 cgaagtttat caaatcgatt ggcttcactt taattgtgac gctttgcatg attgctggac
    11461 aaattggttt tggtattttg attgcgcggg cgttgaacgc aaatattaaa gggaaaacat
    11521 tctttagagc atggttcttt tttccagcgg tcttatcggg cgtggttgtt tctttagtat
    11581 ttaaacaaat ttttaactat ggtttaccaa aaattggtag tatgttaggc attgaatttt
    11641 tacaaaccag tcttttagga acaacatggg gagcgatttt tgcagtaatt tttgttttac
    11701 tttggcaagg aattgccatg ccagtcatca ttttcttagc gggttttcaa agtattccac
    11761 aagaaattat tgaagctgct agaatggatg gggcgaatga taagcaaatt ttttggaata
    11821 ttgaaatcac ctacttatta ccaagtattt caatggtatt tattatggca ttaaaaggtg
    11881 gcttaacagc atttgatcaa atttttgctt taactggtgg tggaccaagc gatgccacaa
    11941 catctttggg gttacttgtt tataattatg cttttaaaaa caatcagttt ggttatgcga
    12001 atgctattgc tttagtctta tttatcttaa ttgggattgt ttcactggca caattaaaat
    12061 tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa acatcaaaag aaaaaaatat
    12121 ttctaaatac ttgcttttat tattaggggg cttcatgatt ttagttccct tagtttcaac
    12181 gctattcggt tcatttaaaa caacacccga tattatgaat aatttctttc gttttcctaa
    12241 tccagcgact ttggaaaatt tccgaagatt gttagcggat ggaattggtc attacttttg
    12301 gaattcggca atcatcacaa tcattgcagt agttgctgtg acagcaatta ttcctatggc
    12361 tgcttattca ctagcaagaa aaatgactcg tgggaaagca tacggattga tgtatttatt
    12421 tttaatttta ggaatttttg ttcctttcca agtaattatg attccgatta ccgttatgat
    12481 gagtaaaatt ggtttagcaa atatgtgggg attaatctta ctctatctca cttatgccgt
    12541 accacagaca ctatttttat atgttggttt tattaaacta aatgtgccta tcagtctgga
    12601 tgaggctgct gaaattgatg gggctagtca ctttacaact tatttcaaaa ttgttttccc
    12661 aatgttaaaa ccaatgcatg cgacgactat tattcttaat gctctatggt tttggaatga
    12721 tttcatgcta ccattattaa ttttaaatcg tgattctaat atgtggacac taccattgtt
    12781 tcaatataat tatacgggac aatattttaa cgactatggt ccaagttttg cttcctatac
    12841 agtaggaatt gtcacgatta cactagttta tcttgctttc caaagaagta ttatagctgg
    12901 aatgagcaat ggagcagtga aataaccaat aaattattct cattataaag gagttcataa
    12961 acttatgatt atatttgaca atcacacaca aacatttcat ttacaaaata aaaaaattag
    13021 ctatctatta ggaatcgaag aaaataatta tttaacgcat ctttattttg gaaaaaagat
    13081 tacgcactat tctggtgggt atcgctaccc ccgaacagat cgttccttct cacccaacaa
    13141 tgcaaatgcg caagatcgtt tattatcttt agacacatta ccattagaat ttccaggata
    13201 tggacatggt gattttagag aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt
    13261 aaatgatttt cgttatgata gctacgaaat tcaaaaagga aagcccagct tagcgggatt
    13321 gccagcctta tatacggaag atgatgatga agctgaaacg ctgattattc aattgaaaga
    13381 cgcgattagt ggattgactt tacagttgtt ctacacaatt tatcaaaatc acagtgtaat
    13441 tattcgttct acgcaattag taaataatag tgatgaaaca gtggagatta accgcttagc
    13501 tagtcaatca attgattttc caaatcgtcc actcgagtta attcatttaa atggtacctg
    13561 ggcaagagaa cgtcaaatga ctagagaaaa aattcataca gggataaaag tattagatag
    13621 taaacgcgga tcaagcagcc atcaacaaaa tccgtttgtt gctttagtag atgccaaaac
    13681 agatgaattt caaggagagg catatggatt taatttagtt tatagcggta atcacgaaac
    13741 ggtgattcaa aaagatccat ttgaccaaac acgggtcatt atgggaatta attcatttaa
    13801 ttttggttgg caactaaaaa ctggcgactc atttcaaacg ccagaagtcg tgatggttta
    13861 tagtaatgaa ggtctgaata cgttatcaca gacgtatcat gaattgtata atcaccgttt
    13921 aattcgtgga aactatcgtt taaaagaacg tcccacgtta atcaacaact gggaagcaac
    13981 gtattttgat tttaacgaag aaaaaatcat gaatattgtc gatgatgcga agaacttggg
    14041 cattgaaatg tttgttttag atgatggttg gtttggcggt cgtgaaaatg acttcacttc
    14101 attaggtgat tgggttgaaa cagaaggcaa acttgcttgt ggtttggaaa atttagccaa
    14161 gcaagtgcat gataacggga tgaaatttgg gatttggttt gaaccggaaa tgatttctga
    14221 agatagtgaa ctatttcgag cacatccaga ttgggcattg ggtattccag gtcgtgaccg
    14281 ctcaattagt cgaaatcaat atgttcttga tttttcaaga caagatgttc gtgataatat
    14341 ctatcaacaa atgacggcga tttttgatcg tgtaccgatt gattatgtga agtgggatat
    14401 gaatcgaaat atgacagaag tctattctgt cttgttggac tcaacgatgc aaggtgaagt
    14461 ggctcatcgt tatatgttag ggttatatga atttttagaa aagttaacaa cgaattatcc
    14521 aactatttta tttgaaagtt gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta
    14581 ttacatgcca caaacttgga cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca
    14641 atatggaacc agcttagtgt atccaatttc aagtatgggt gcgcatgttt cagcagtccc
    14701 aaatcatcaa acaggaagag aaacgagttt aactattcgt ggcaatgctg cgatggctgg
    14761 tgtttttggt tatgagctag atctaacgca attaacttct gaagaaaaag cagaaattaa
    14821 agaacaagtt gccttttata aaaagcatcg ccaacttttg caatatggaa ctttttatcg
    14881 cttggaaagt ccgtttgaag gcaatgatac tgcatggatg tttgtctcaa aagaccaaac
    14941 agaagccatg gtcttttatt tccgagtatt agcagaagct gcgcatccat tagtcacatt
    15001 gaaattagca ggattagcag aagaagcaac ttatcaaatg gaagatgtaa catttgctgg
    15061 cgatgaattg atgaatctcg gtttttatat tgactcagaa ctacatggtg attatgcgac
    15121 acaacgcttt tatttgaaac agatatagta gcttgaattt acaatataag tgcaacacca
    15181 aaagagactc attccaaacg aagaaaatat gttttattta cacgcattta ctaaaaagca
    15241 gcctgatttg aattgggaaa atgaagaagt acgtgaagaa atctatgcaa tgattaacta
    15301 ttggttagat aaaggactag gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa
    15361 actagagtat ggcatctttg aatctgatgg agaagatggg ttagccttca ttgggccctg
    15421 gattcttaat cagtcgggca ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa
    15481 accacataat agtatgacgg tagcagaagc cgatgttcct aatgaacgtt tagatgaata
    15541 tattggtgaa gatggctatt attcgatggt ttttgatttt agttatacgg atattgatgt
    15601 acctgaaact ggtgaatggt ttaaagatag ccaatggaca tggacggata tgcgtaccaa
    15661 tatttttacg aatcaattag ttacgcaaga taaaggttgg ggcgccttat atttagaaaa
    15721 ccacgatcaa ccacgttcaa ttaataaata tattccagaa gaatggatca atgattatag
    15781 taaaaaaatg ttggcgacgc tttttatgtt actaagaggt acccctttta tttatcaagg
    15841 acaagaacta ggaatgacca acatagaaat ggcatcatta gaagattttg atgatgtagc
    15901 tacgcattct cagtataaac gggcattaga atatggattg gcaccagagc aagcattgaa
    15961 agctgtcaat aaacgaagtc gggataattc tcgcacacct atgcaatgga atacgcaaaa
    16021 aaatgccaga ttctcaactt ctgataatgt atggttaaaa gtaaatccta attatccaga
    16081 actcaatgca gaagctcagt taaccaatca acagtcggta ttcaattatt atcatcaatt
    16141 aattaaatta agaaaaaata gtgtatacaa agacgtgcta atctacggac aatttctacc
    16201 agtgaaagat aaaaatgaac atattttgct ttatgaacgc caattagaag ataaaatcgt
    16261 attagttatc ttaaacgtta cagcaacaga acaagaatat tatgtagatg aacagtacca
    16321 acaagtatta atcaataatt atgaagatgt tttattagat caaagtcgaa aattgcgttt
    16381 acgaccgttt gaaagtattg tattagctaa ttatggagga caattatgaa aattaaaaac
    16441 gaagcaatgc tcatcaccta tgcagatagt ttaggaaaaa acatgggcga attaaaacaa
    16501 gtcttagaca aacatttaca aggtgttatt ggtggtgtac atctgttgcc tttttttcca
    16561 tcgacaggtg atcgaggctt tgcgccgagt gattatacga cagttgatcc agctcttgga
    16621 acatgggaag atgtggaagc attaggtgaa gattattatt tgatgtttga ttttatgatt
    16681 aatcacattt ctcgtgaatc aaaatttttc caagatttca aaaaaaacca tgagcagtca
    16741 ccatacaaag aaatgtttat ccgtattcat gaatttttcc cagaaaatcg accaacacaa
    16801 gaagatattg atttaatcta caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt
    16861 gcggatggta cgacagaaga ggtttggaat acctttgggg aagaacaaat tgatttagat
    16921 gtgaccaaag aagttgtgaa agaatttatt cgagaaacca tcaaagatat ggcgaaccat
    16981 ggctgttctt taattcgatt agatgcgttt gcttatgcga ttaaaaaatt agataccaat
    17041 gatttcttcg tggaaccaga tatttgggaa cttttagacg aagtgcgtca agaagcagcg
    17101 aaatatgacg tcgaattatt accagaaatc catgagcatt attccattca aatgaaaatt
    17161 gccaatcatg attattatat ctatgacttc gccttgccaa tggtcactct gtattcactt
    17221 tatagtggaa aatcagaacg tttagccaat tggttgaaaa tgagtccgat gaagcagttt
    17281 acaacattgg atacgcatga tggaattggt gttgtggacg ctcgtgattt attaacagat
    17341 gaagaattgg attacacctc tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc
    17401 tattcaagtg ccaattacaa taatttagat atttatcaaa ttaacagcac ctattatagt
    17461 gcattaggcg ataatgaccg tagctatctg ttagcacgtg cgattcaagt atttgcgcca
    17521 gggattccac aaatttatta cgttgggtta ttagcaggaa aaaatgatat tgaactgttg
    17581 gaagcaacga aagaagggcg taatattaat cgtcattact atgatttagc cgaaattgaa
    17641 gaagaagtgc aacggccagt gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa
    17701 tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa cgccatcaga atcagaaata
    17761 gtgattacac gtaaaaataa aaacggtagt gatagtgcaa cattaacggc taacttggat
    17821 acgaaagaat ttatcattga gtctaaagat ataaaaattt tcactagtgt tgcattaaaa
    17881 ttttaaccta ttccatagaa caaaaaatcc tttatactag cacttggatg acttatccaa
    17941 gaccaataaa aaggactcat acatggataa gtataaaaca aatacagcat ttaataaatg
    18001 gttttctgcc ataaaattag gaaaactttc tgtcaactcc caaaaatgta tttttgatta
    18061 caattcgtat cgtaaaaaac tgtcttttga agctgtgctc aagctctttc tctacgcagt
    18121 taatgaagaa aaagaaagtt tgcgtgattt gagtacagct cttatcgacc gatcgctcca
    18181 actagaaacc gatgtgtcat cgattagtca tacccaattg tctcgagcat ttaatgcact
    18241 tgaacctaaa gtgttagaag aaatttttca acaactgtta gaaaaagttc aacatcaaat
    18301 caaaccaacc aagcgaaatc atctgtatct gattgattcc agtacgtttt cgcttagttt
    18361 aaaacgtcat aagtgggcga ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa
    18421 ctattgttat atggacgata ctacgatgta tccaactgat tttaccttaa caaatgccca
    18481 tgaacatgat ctcaatcaac taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt
    18541 tgatcgtggg tattaggact ttgaaaagat ggatcagatg cattgggatg gctatttctt
    18601 cgtcactcgg attaaaaaaa acaccaaagt tcacgtgata gatactctgg aaacatcgcc
    18661 agaaacagaa attctacgag acgagctggt tcgtttagga tctaaaacct atctaaccgc
    18721 caatttccga ctagtcactg ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa
    18781 tcgaatggac gtctcttcaa aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga
    18841 attgtttttc aagcacatca aacaacacat gaccattaaa accttctttt ctcaaagtga
    18901 aaaaggcgtg cagaatcaat tgattttaac gatgatttcc gctctcctca cgtttctcat
    18961 caaattggag acgaaaacgg aaaaatcagt gttccaaatc aaacgattct ttcgttattt
    19021 actgtttcag ccctttgaat gttgtctaga aaaattaatt cccacatgag aaaaaagaaa
    19081 tttggctcgt tacttttggt tttcagttga ataacagata aaaaattttc tggaaaaagt
    19141 catcctctgc gtcagcacag ttttttttga cttattttca gaggaaatag ttttcagaat
    19201 attctaaaat gttttatgca acactagtga tattttttag aattagattt ctaatgtgca
    19261 ttttttgagt attaaggagc aagtatacaa agatttataa agctgattaa atttgaataa
    19321 aaatatgcag gaacgctact atttttagta gtgttctttt ttatatttga ataaaaaatg
    19381 tggtggttaa atggtggtta taacaaaaaa tgaattaatg aaattaggtt atgggaagta
    19441 tcagtctgag agaatcatac gacaggcaaa agcaattatg gcttcaaaaa gctataccta
    19501 ttacgaaaat agaagattag gactagtacc tgttgaatct gtagaagaag ttttaggtat
    19561 gaagatagag gtagaggagg attttcagaa tcatggttaa ttttaaaaaa gataaaaatg
    19621 ggaaatatca ttttactgct agtttgggat tcgacaaacg aacagggaaa agagtacaaa
    19681 aaaacgtaga ggggttaaaa cagttaaaga agaaaaaaag cgtataccga acttgtttct
    19741 aattatggaa aagaagcaga aacttctcaa agtactatgt tgtttgagga gtttttttat
    19801 aaattattcc taccttatta tcaagggaaa gtagaagaaa gaacatataa aaatcggtta
    19861 tctacaatca aaaaaacttt tcttatttct ttaaaatgga gttaagagaa atacgtccaa
    19921 ttgacattca aaagtggcaa aataatttat tgagcaaata taaaaattct tatgtaagaa
    19981 acatgtatgg gatttttcag atgtcattag atcgagcagt tgttttggga ctacttgaaa
    20041 aaaatcctgc aaaagttatt ggaaatgtga agaaaaggaa aactacagtt gatttttgga
    20101 caaaagaaga atttacaaaa gttatttcaa ctttttatat tgatgatttg taccaacatt
    20161 ttggttttat ttgtatgtgg ttacttttta tgaccgttat gcgtttggga gaagccactg
    20221 cattaacttg gaaagatatt gattttgaaa ataaaaactt gcgtgtgaat aaaacactct
    20281 attataagag agccaaaacc tatgagtttg tagaacccga aacggcttct agtattcgta
    20341 caatcgaact agacgacaat acaattacct ttttaaaagc ttggaaggag cgtcaagttt
    20401 caataggagg catgtcgttt gtactttctt ataatggaac acctactcag aaatatacaa
    20461 tccgtcatat tatgagaaga catgcaacga tcgttggtgt tcatataatc cgcatacacg
    20521 gattaaggca tagtcatgct tctttgctta tatcacaata aaaagatgca cttcgaatta
    20581 aagaaaggtt aaaacatgct gatgtacaaa cgactttagg aacttatggt catttgtatc
    20641 taaattcatt gagagagata acaaatgatc ccacagatta tatccaaact aatttttcta
    20701 aaatcaaacc gacacctatt tacgataata aaaaataaat gataaacaaa agtaggatca
    20761 caccaaaaga aacacactag aaaaactata atacaggctt ttctagcgtg ttgtttctta
    20821 tttccgccta tttatacatt atactcataa agcatttaat gcatcgatcg tagccgcatt
    20881 gtctatatgg gtatataaag ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt
    20941 taataccaat gaatttgtct gttggtagag tctagtagct aatgtatgac gcagtttgtg
    21001 agggctgact tgaattctaa agccttttga atattttgct accatccgtt cgattgcaat
    21061 accggatata cgcttggctt ctccttggta aattgctaga aataaagctt tttcgttttc
    21121 tgttacttta tatcgttctt tccgaatagc tagataatta gctaaatatt ccattgcgaa
    21181 tgctgctata ttgacagcat ctcttttatt tccttttcta gttactacca cattattctt
    21241 atctaaagat aggtcatcta aattaatatt tataacctcg gagagtctca atccagatcc
    21301 taaaatcagt gcattgatcg ccaggtctct ttctacatct cgttgataat agatcaaagc
    21361 tcgtttactc aaagtatttt tatattcatt ggcaatataa tctagataat tttgtgtatc
    21421 cttatctaga aataattttt tctcaatggc attggcacgt gcagctaacg tagttttatc
    21481 tgtgatggtt gctacttgat ccataggatt attgtctaag tatggttttc ctagaggatt
    21541 attggaactt ttcgaaagat atttaaatag aacctttaat gccgtgatat ttctattgat
    21601 agtagaagta gatagaccta agttattatt atctttgagc ttagtttttg ttgtctcttt
    21661 ttttacattt tcttttgtgc gacgtttcaa atgagatttg tacaagttta ggtcataggc
    21721 agttaactgt cttatcgagg tgatagaagt atcaggaacg atagactcac tcagaagcca
    21781 ttcataaaag agcttataat tttttagata ttctaaagtg gttcgaggtg attgatcttt
    21841 atcctcacga tcttgaacaa aatcttttat ataatcgggc atatgaagta tgattgtatc
    21901 tattttttga tatatttctg tatttttcat gtcattcatc ctttcaataa gtaggggaga
    21961 agagtatctc tataattgga ctataccaat tatagaatgt tttgtcaatt ggtatgctct
    22021 aaaatttatt aatttgtttc aaaaaaagca tataaacagg tatacgttta ataatttaaa
    22081 ataaatggta tacataaata gggaaggaaa tagaagggag caatctaaat tttttgaatt
    22141 ttcttaatta attatataga aagaacaatc tttctataga gttagacatg agagggacta
    22201 tgcttgattt aaaatcaaca agcgtatata ctgtatatac aaagaaaaag gagggagaag
    22261 actatggcaa ttaaagaaag aaaatttagg gaagtgggga actcagtagt attaactatt
    22321 tcaaaggagt ttcttaaaga aaatgggtta aatcccggag atacggtttt tttagatgaa
    22381 gaaaaacttc gagcggcaat tacaaaaaaa caagccttat ctgatgaaat tgatttagca
    22441 atatctcaag cattaactga atatgatact acttttagag agcttgtcga taaatgagta
    22501 ttcattacct agaagccaaa gatattataa aaatgaatgt aatccaaata aaaaaatact
    22561 ctcctaatga accccttggt gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac
    22621 ttcgagcctc agcttttggt gaagaggttt atccttcgat ttatgaaaaa gcagctatcc
    22681 tacttataca attgataaag aaacatcctt ttcacaatgc aaataaacgt actgctttct
    22741 tagctacttt tgtctttttg aaaatcaatg gacagctttt agctattgat caaaaagatg
    22801 ctgtaaattt agcggtatat attgctacgt atgataagga tttcgatcaa ctaaaatatg
    22861 aagtgatcaa cactataaag acacatacgc agcccattta aggagttact gtatgtgtct
    22921 ttatagggtt attagctgct atagcgaaat aattgaagta aattacaaat agctataatt
    22981 caaatccaac tatatagaaa acgtaaattt tctatatagt tggattaaga taacgactat
    23041 ttattttatc atatttttac tataccctat acttttactt ggtatattta tgaataatcg
    23101 ca
//
LOCUS       DADVXN010000040        22654 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.041, whole genome shotgun sequence.
ACCESSION   DADVXN010000040 DADVXN010000000
VERSION     DADVXN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.041"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            133..3660
                     /locus_tag="KB942_001341"
     CDS             133..3660
                     /locus_tag="KB942_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBB6851777.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="KB942_001342"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="KB942_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBB6851778.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="KB942_001343"
     CDS             7448..7789
                     /locus_tag="KB942_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6851779.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="KB942_001344"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="KB942_001344"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBB6851780.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="KB942_001345"
     CDS             9146..11566
                     /locus_tag="KB942_001345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBB6851781.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="KB942_001346"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="KB942_001346"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBB6851782.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="KB942_001347"
     CDS             13000..13737
                     /locus_tag="KB942_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6851783.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="KB942_001348"
     CDS             13751..14566
                     /locus_tag="KB942_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6851784.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="KB942_001349"
     CDS             14579..15226
                     /locus_tag="KB942_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6851785.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="KB942_001350"
     CDS             15242..15898
                     /locus_tag="KB942_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6851786.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="KB942_001351"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="KB942_001351"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBB6851787.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="KB942_001352"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="KB942_001352"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBB6851788.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="KB942_001353"
     CDS             18240..18758
                     /locus_tag="KB942_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6851789.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="KB942_001354"
     CDS             18865..19365
                     /locus_tag="KB942_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6851790.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="KB942_001355"
     CDS             complement(19645..20565)
                     /locus_tag="KB942_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBB6851791.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="KB942_001356"
     CDS             20730..21743
                     /locus_tag="KB942_001356"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBB6851792.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="KB942_001357"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="KB942_001357"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBB6851793.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7545 a         3950 c         4824 g         6335 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaa
//
LOCUS       DADVXN010000041        22474 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.042, whole genome shotgun sequence.
ACCESSION   DADVXN010000041 DADVXN010000000
VERSION     DADVXN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.042"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(200..718)
                     /locus_tag="KB942_001358"
     CDS             complement(200..718)
                     /locus_tag="KB942_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6851794.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(720..1337)
                     /locus_tag="KB942_001359"
     CDS             complement(720..1337)
                     /locus_tag="KB942_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6851795.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1334..2188)
                     /locus_tag="KB942_001360"
     CDS             complement(1334..2188)
                     /locus_tag="KB942_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6851796.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2185..3096)
                     /locus_tag="KB942_001361"
     CDS             complement(2185..3096)
                     /locus_tag="KB942_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851797.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3137..4471)
                     /locus_tag="KB942_001362"
     CDS             complement(3137..4471)
                     /locus_tag="KB942_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6851798.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4679..5509
                     /locus_tag="KB942_001363"
     CDS             4679..5509
                     /locus_tag="KB942_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBB6851799.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5753..6071)
                     /locus_tag="KB942_001364"
                     /pseudo
     CDS             complement(5753..6071)
                     /locus_tag="KB942_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6193..6387)
                     /locus_tag="KB942_001365"
     CDS             complement(6193..6387)
                     /locus_tag="KB942_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851800.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6892..7077)
                     /locus_tag="KB942_001366"
     CDS             complement(6892..7077)
                     /locus_tag="KB942_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851801.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7927..8268)
                     /locus_tag="KB942_001367"
     CDS             complement(7927..8268)
                     /locus_tag="KB942_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6851802.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8268..8498)
                     /locus_tag="KB942_001368"
     CDS             complement(8268..8498)
                     /locus_tag="KB942_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6851803.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8785..9123
                     /locus_tag="KB942_001369"
     CDS             8785..9123
                     /locus_tag="KB942_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851804.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9328..9567)
                     /locus_tag="KB942_001370"
     CDS             complement(9328..9567)
                     /locus_tag="KB942_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6851805.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9543..9890)
                     /locus_tag="KB942_001371"
     CDS             complement(9543..9890)
                     /locus_tag="KB942_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6851806.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10622..11203)
                     /locus_tag="KB942_001372"
     CDS             complement(10622..11203)
                     /locus_tag="KB942_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851807.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11386..12012)
                     /locus_tag="KB942_001373"
     CDS             complement(11386..12012)
                     /locus_tag="KB942_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6851808.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(12034..13365)
                     /locus_tag="KB942_001374"
     CDS             complement(12034..13365)
                     /locus_tag="KB942_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBB6851809.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13520..13921)
                     /locus_tag="KB942_001375"
     CDS             complement(13520..13921)
                     /locus_tag="KB942_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6851810.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(14007..14213)
                     /locus_tag="KB942_001376"
     CDS             complement(14007..14213)
                     /locus_tag="KB942_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851811.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14308..15810)
                     /locus_tag="KB942_001377"
     CDS             complement(14308..15810)
                     /locus_tag="KB942_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBB6851812.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15828..16283)
                     /gene="argR"
                     /locus_tag="KB942_001378"
     CDS             complement(15828..16283)
                     /gene="argR"
                     /locus_tag="KB942_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6851813.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16381..16998)
                     /locus_tag="KB942_001379"
     CDS             complement(16381..16998)
                     /locus_tag="KB942_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBB6851814.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17219..18592
                     /locus_tag="KB942_001380"
     CDS             17219..18592
                     /locus_tag="KB942_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6851815.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18667..19779
                     /locus_tag="KB942_001381"
     CDS             18667..19779
                     /locus_tag="KB942_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBB6851816.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19851..21488)
                     /locus_tag="KB942_001382"
     CDS             complement(19851..21488)
                     /locus_tag="KB942_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBB6851817.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21656..22264)
                     /locus_tag="KB942_001383"
     CDS             complement(21656..22264)
                     /locus_tag="KB942_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6851818.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6584 a         4559 c         3892 g         7439 t
ORIGIN      
        1 aaatgatatc ctttttagtt aaaaaacacc aattatacaa catttattat taaaattaaa
       61 ttataaaatt ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa
      121 acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg
      181 ctttattcgt acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta
      241 aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag
      301 cgatcaaaca agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg
      361 cagctactga taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt
      421 tacttgtgta aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt
      481 ttttgataaa aagcaaacca acaaaaaaat agatcaaact gccgataaga atggctagac
      541 catattctct agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac
      601 caccattaga gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc
      661 gttgcttggc gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt
      721 tattcctcct ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa
      781 taaaacaaga aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg
      841 acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac
      901 aagaaaaatg cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa
      961 ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca
     1021 attcccccac cccaatagcg gaaaatctga taatcgatca atagaagaag aaaataaaca
     1081 cttgctaaac tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat
     1141 agtgaaccga gctttttctc ttcatttttt ttgtaatgat aaatcatata cagaactgtt
     1201 cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa
     1261 tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca
     1321 atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac
     1381 gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca
     1441 gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt
     1501 atttttcttg atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga
     1561 aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc
     1621 aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg
     1681 ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga
     1741 tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat tgagggatca
     1801 tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc
     1861 caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga
     1921 tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg
     1981 caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac
     2041 gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca
     2101 aaatgattcc tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca
     2161 cactggtctt tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt
     2221 aggtgcaagc ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga
     2281 catagccatc gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt
     2341 ataaaaaagc aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc
     2401 ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt
     2461 gattaatggc aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc
     2521 tgattcatat aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc
     2581 tccagaactc catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag
     2641 ccaagctggt ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg
     2701 agaaagcagc tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata
     2761 gaaagctgta cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa
     2821 cacactgaaa atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa
     2881 cgatttccaa aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa
     2941 attcatcttg gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg
     3001 ccctagcata aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct
     3061 tgcttctttt ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta
     3121 gacagtcaaa aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat
     3181 ctgccaatcc ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca
     3241 ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt
     3301 tttcgtccgc catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta
     3361 aattctcatt ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt
     3421 gtacttctgg agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc
     3481 cgtatggtac ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt
     3541 tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag
     3601 agtaataatt gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata
     3661 caccagaccc aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc
     3721 agtcttggat ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat
     3781 ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat
     3841 tccataatgg ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac
     3901 gtgctgtttc ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt
     3961 ctttaaataa ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat
     4021 aaggatcacc ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt
     4081 ctttttctac atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg
     4141 aatttatgtc ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt
     4201 tagtccagat gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt
     4261 gattgaaatc ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg
     4321 accagaaatc aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg
     4381 aactggcatc gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg
     4441 ccagcaaccc tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga
     4501 tgccggtttt aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat
     4561 tctaaaaaaa ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat
     4621 tttttaatct ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat
     4681 gtttagacac cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat
     4741 cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca
     4801 gtacctgttc caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga
     4861 cattgcaaca gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt
     4921 agagggtgcg cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc
     4981 cattatctct ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc
     5041 tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc
     5101 agactttaga gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta
     5161 cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag
     5221 ctatcatcaa aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat
     5281 cttagtccca agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta
     5341 taacttctca gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga
     5401 tggcgaaggc gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt
     5461 agaaccagta acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg
     5521 tagaaacacc cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat
     5581 cataaccacc cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag
     5641 cgcctaaaga cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg
     5701 cccacctctt aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc
     5761 gtacactcag cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga
     5821 tggtcgcttc attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat
     5881 acccaaattt atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga
     5941 tattgattat aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac
     6001 atctactttg tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt
     6061 ttgtatttca atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg
     6121 ctgcttcact gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta
     6181 cggcaatcta ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag
     6241 aaataaagcc gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga
     6301 aatcccgata acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt
     6361 atagaataga actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca
     6421 caaattcacg gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga
     6481 ctttccctac aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct
     6541 aaatctaaat tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca
     6601 gtatacataa ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt
     6661 gcaggagtac tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga
     6721 cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact
     6781 acatgcacat ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg
     6841 ggaattgcaa tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt
     6901 tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc
     6961 attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa
     7021 catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca
     7081 tagacacttc cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa
     7141 aagtgactca aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta
     7201 cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg
     7261 ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc
     7321 gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa
     7381 gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc
     7441 taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg
     7501 catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct
     7561 tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct
     7621 aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa
     7681 agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac
     7741 acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt
     7801 ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt
     7861 acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag agtttactta
     7921 caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa
     7981 gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat
     8041 tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat
     8101 agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga
     8161 gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat
     8221 ccatacaata tcaccttttt taggaatata aattctttct tctgacacta taaaatttct
     8281 ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca
     8341 ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca
     8401 acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact
     8461 acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa
     8521 gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt
     8581 tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt
     8641 tctttaacta aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa
     8701 tactatagct aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt
     8761 tcaaagacaa caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt
     8821 tgctctgaca aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact
     8881 gttcggatca aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga
     8941 caagactttg ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta
     9001 catgatccaa ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa
     9061 tatacaactg ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca
     9121 taatgataaa catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat
     9181 agaagctata cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg
     9241 agcatttctt ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc
     9301 cgctctttct ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg
     9361 ctgagtaatg atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc
     9421 agtcaagaca agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt
     9481 cattatgatt gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata
     9541 cactattttt tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg
     9601 aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct
     9661 gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt
     9721 tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa
     9781 acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa
     9841 taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct
     9901 cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca
     9961 atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc
    10021 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa
    10081 attgttcctt gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc
    10141 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac
    10201 aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg
    10261 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt
    10321 ttatttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac
    10381 ggtgttcaat cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    10441 tttgccgttt gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac
    10501 ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc
    10561 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt
    10621 attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa
    10681 gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca
    10741 acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga
    10801 tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt
    10861 cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga
    10921 tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct
    10981 gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat
    11041 ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc
    11101 ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa
    11161 taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc
    11221 atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa
    11281 aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag
    11341 atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc
    11401 taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc
    11461 atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc
    11521 actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag
    11581 aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc
    11641 aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc
    11701 gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat
    11761 ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg
    11821 gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat
    11881 ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc
    11941 accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt
    12001 attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc
    12061 actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt
    12121 catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc
    12181 tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa
    12241 tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg
    12301 tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact
    12361 gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat
    12421 aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc
    12481 aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg
    12541 atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc
    12601 agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt
    12661 gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc
    12721 gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg
    12781 aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga
    12841 tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc
    12901 ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg
    12961 taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt
    13021 ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat
    13081 cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt
    13141 caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat
    13201 caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga
    13261 cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc
    13321 gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct
    13381 tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca
    13441 tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg
    13501 ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg
    13561 ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc
    13621 tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat
    13681 tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca
    13741 tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat
    13801 ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg
    13861 cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca
    13921 ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag
    13981 taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt
    14041 tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa
    14101 atagcaatct tttctacata atcttttata tgattgattt taagatacat atataccaga
    14161 aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc
    14221 tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca
    14281 agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa
    14341 atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt
    14401 ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt
    14461 tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat
    14521 ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg
    14581 tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc
    14641 tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat
    14701 ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc
    14761 gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt
    14821 gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa
    14881 tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt
    14941 taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc
    15001 actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt
    15061 tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg
    15121 attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct
    15181 attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt
    15241 ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa
    15301 atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt
    15361 tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga
    15421 ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc
    15481 agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa
    15541 ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag
    15601 tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc
    15661 ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat
    15721 gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga
    15781 tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt
    15841 ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat
    15901 gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc
    15961 gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg
    16021 atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat
    16081 ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc
    16141 atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc
    16201 cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt
    16261 gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata gcaaaagata
    16321 agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg
    16381 ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct
    16441 tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc
    16501 gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa
    16561 gatttgggca caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc
    16621 cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg
    16681 ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata
    16741 cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg
    16801 aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc
    16861 tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga
    16921 ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg
    16981 ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat
    17041 acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat
    17101 aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt
    17161 ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat
    17221 gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt
    17281 tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt
    17341 tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat
    17401 cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc
    17461 ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt
    17521 cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt
    17581 ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac
    17641 tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca
    17701 ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat
    17761 cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag
    17821 cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac
    17881 agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg
    17941 aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg
    18001 cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt
    18061 gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct
    18121 tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata
    18181 tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt
    18241 attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat
    18301 gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga
    18361 agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt
    18421 ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta
    18481 tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct
    18541 cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata
    18601 tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc
    18661 ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt
    18721 tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact
    18781 aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat
    18841 cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat
    18901 caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa
    18961 ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg
    19021 ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt
    19081 atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta
    19141 gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct
    19201 tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc
    19261 ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa
    19321 gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg
    19381 cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc
    19441 catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact
    19501 aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc
    19561 cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat
    19621 gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat
    19681 atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa
    19741 aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa
    19801 ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat
    19861 ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc
    19921 tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac
    19981 gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga
    20041 ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt
    20101 tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc
    20161 tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa
    20221 ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc
    20281 gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg
    20341 atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt
    20401 tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt
    20461 ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc
    20521 acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt
    20581 ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa
    20641 gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt
    20701 gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca
    20761 tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca
    20821 agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta
    20881 ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga
    20941 tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag
    21001 atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct
    21061 tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt
    21121 gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat
    21181 ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca
    21241 atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca
    21301 tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct
    21361 gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat
    21421 cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt
    21481 gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt
    21541 cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa
    21601 aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt
    21661 accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta
    21721 catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag
    21781 catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt
    21841 ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg
    21901 cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg
    21961 tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat
    22021 ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat
    22081 ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag
    22141 ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta
    22201 caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat
    22261 tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat
    22321 ttagaaatat ttcataggta aaagaaacat gacatttaat gacaaaataa cgaattattt
    22381 aatatttgta acatttaaac aaatattttt ttgatacaaa accataaaag ccttgttgta
    22441 taagcaaagt aatcgttttt ctatgttttt ttat
//
LOCUS       DADVXN010000042        22205 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.043, whole genome shotgun sequence.
ACCESSION   DADVXN010000042 DADVXN010000000
VERSION     DADVXN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.043"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            271..2106
                     /gene="tdc"
                     /locus_tag="KB942_001384"
     CDS             271..2106
                     /gene="tdc"
                     /locus_tag="KB942_001384"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6851819.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2411..3889
                     /locus_tag="KB942_001385"
     CDS             2411..3889
                     /locus_tag="KB942_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6851820.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3855..5186
                     /locus_tag="KB942_001386"
     CDS             3855..5186
                     /locus_tag="KB942_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6851821.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5300..7852
                     /locus_tag="KB942_001387"
     CDS             5300..7852
                     /locus_tag="KB942_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBB6851822.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7859..9298)
                     /locus_tag="KB942_001388"
     CDS             complement(7859..9298)
                     /locus_tag="KB942_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851823.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9644..10006
                     /locus_tag="KB942_001389"
     CDS             9644..10006
                     /locus_tag="KB942_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBB6851824.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10193..11050)
                     /locus_tag="KB942_001390"
     CDS             complement(10193..11050)
                     /locus_tag="KB942_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6851825.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11170..12081
                     /locus_tag="KB942_001391"
     CDS             11170..12081
                     /locus_tag="KB942_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6851826.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12088..13739
                     /gene="mdcA"
                     /locus_tag="KB942_001392"
                     /pseudo
     CDS             12088..13739
                     /gene="mdcA"
                     /locus_tag="KB942_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13841..14140
                     /locus_tag="KB942_001393"
     CDS             13841..14140
                     /locus_tag="KB942_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBB6851827.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14130..15806
                     /gene="mdcD"
                     /locus_tag="KB942_001394"
     CDS             14130..15806
                     /gene="mdcD"
                     /locus_tag="KB942_001394"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBB6851828.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15810..16403
                     /locus_tag="KB942_001395"
     CDS             15810..16403
                     /locus_tag="KB942_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBB6851829.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16427..17503
                     /locus_tag="KB942_001396"
     CDS             16427..17503
                     /locus_tag="KB942_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB6851830.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17528..18397
                     /gene="mdcB"
                     /locus_tag="KB942_001397"
     CDS             17528..18397
                     /gene="mdcB"
                     /locus_tag="KB942_001397"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBB6851831.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18508..19272
                     /locus_tag="KB942_001398"
     CDS             18508..19272
                     /locus_tag="KB942_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBB6851832.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19761..21854
                     /locus_tag="KB942_001399"
     CDS             19761..21854
                     /locus_tag="KB942_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6851833.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21879..22181
                     /locus_tag="KB942_001400"
     CDS             21879..22181
                     /locus_tag="KB942_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6851834.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7187 a         3790 c         4732 g         6496 t
ORIGIN      
        1 tgtttagtga atgaaggaca gtcattttat ttgtttgttc gcttttttta ggttatctat
       61 tttagaagac tgatttattg aaaaattgtt cgcttttttg cgttgaatta tcagaaaaat
      121 ccgactttat ttatttttgt ttgtaaaaat ctacgtggat ttgatgttat aaaaaaaaaa
      181 ttctcatttt tggcgctata gtgaagaggt gttacagata aacacatgta tctgcaagac
      241 attgaccaac aaaatatgga gggaagaaaa atgaaagata tggatatcaa ggccgtcttt
      301 ataggcgaca aagctgaaaa tggaccagtt tataaaatgt tgttgaacaa gatggtagat
      361 gaacatcttg gatggagaga aaattatatt ccttcagata tgcctgcaat tagcgaagga
      421 gataagctca ctccagatta tctagccacc cgcgaccata tgatagaggt cttggatgaa
      481 gtcagtcagc gattacgcgc aggatcgatc ccatggcatt cagcaggccg ttactgggga
      541 caaatgaatg ctgaaacatt gatgcctgct ttattagctt acaactatgc gatgctgtgg
      601 aacccaaata atgttgcatt ggaatcttct atggcaactt cgcaaatgga agcagaagta
      661 ggacaggatt ttgcttcatt attcaatatg acagacggat ggggacacat tgcagcagat
      721 ggttctattg ctaatcttga aggattatgg tacgcccgtt gcatcaaatc gattccttta
      781 gctgtcaagg aagtgctgcc tgaaaaagtg aaaaaaatgt ctgagtggga acttttgaat
      841 ttatctgttg aagaaatttt ggaaatgacc gaaagcttta cggacgaaga aatggatgag
      901 gtcaaagcag cttcttcccg tagcggaaaa aatatccaaa gattagggaa atggctggtc
      961 cctcaaacaa aacactattc ttggatgaag gcgttagata tttgcggagt cggcttagat
     1021 cagatggttg ctatcccagt tcaagaggat tatcgaatgg atattaatgc tttggaaaaa
     1081 acaattcgtg aattagctgg acaaaaaatc ccaattctag gtgtggttgc tgtcgtagga
     1141 acgacagaag aaggtcaagt agacagtgta gataaaatcg tccaattaag agaaaggtta
     1201 aaagatgaag ggatctattt ctatttacat gttgatgccg catatggcgg ctatgcgcgt
     1261 tcactgtttc taaacgaagc aggagaattt gtgccgtatg cttccttagc tgaattcttt
     1321 gaagaacatc atgttttcca ccactgcgta acgattgaca aagaggtata tgaagggttc
     1381 agagctattt cagaagcgga ttctgtcacg atagatcctc ataagatggg ctatgtccct
     1441 tatgctgctg gaggaatcgt aatcaaacat aaaaatatga gaaatatcat ttcttatttc
     1501 gcgccatatg tgtttgaaaa atcagtaaag gctccagata tgttaggagc ttatattttg
     1561 gaaggatcaa aagctggagc aaccgccgcc gcagtctgga cagcacatcg tgtattgcca
     1621 ttgaatgtga ccggatatgg tcaattgatt ggggcttcaa tcgaggcagc tcaaagattc
     1681 agagaatttc tcgatcatct gacttttact gttaaaggaa aaacaatcga agtctatcca
     1741 ttgaatcatc ctgattttaa tatggtcaat tgggtattca aagaacaagg ctgtacagat
     1801 ttgaacgcta tcaatgaatt aaatgaaaaa atgttcgatc ggtcttctta tatggatggc
     1861 gatgtttatg gtgaacggtt tattacttct catactacat ttacacaaga agattacggt
     1921 gattctccga ttcgttttgt tgaaagaatg ggtctaacga aagaagaatg gaaaaaagaa
     1981 cagaaaatca ctctgctacg tgcggctatc atgactcctt atttgaatga tgatcgaatt
     2041 ttcaattttt atacaaaaaa aatcgccaaa gcaatggaaa agaaactaaa cgaaatcatc
     2101 caatagaact gatttttgtg tgaaagaata acaagagaca tatggagaac agacaattat
     2161 ttgcttcatc aaaacaattg tatttccccc tgtttttcat atattcctcg ccaagatatt
     2221 cttttttata aaacgttcta taagttggat cgttcgtttc atgtatcaaa cccagttcac
     2281 taagatcgtt caatggatgt ttcggtgata ctgggttttg tactgaatcg atgaaacctt
     2341 gtcatttctg ttcttgaaca aaaataaaag aaaacaaaaa catatgtcgc tattgaaaga
     2401 aggaataaag atgaaagata ctggttcatc aaagcaaatc acgaccatga cctttatcgg
     2461 aatgacctgc gcccttgtag caagtgtacg aaatattcca gatgtagcag caacgagctg
     2521 gacgatgatt ttttacatgc tggttgcagt attattgtac gcttttccaa tctcattgat
     2581 ctctggtgaa tttgctggaa tgtttcccca aaaaggtggt ccagaacttt gggtatcgaa
     2641 tgctctagga aaaaaatggg ggttcgttgt ctcatggctg ttatgggtcc aaatgtttcc
     2701 tgggatggtg atggtcgctt cagcactcgc tccgttgttt ggtaatatta tcgataatgt
     2761 gccgcttggt ttgaacagca aatttacatt agttgtcatt ttagtcgttt attggatcat
     2821 cacttttttg aatttgaaat ttgatatggc aaaaatcggc ggaaaagtcg gtgtttggtt
     2881 aggattgtac gtgcctttga cgatgatgct cttattaggg tttgctgcgt ggatcaaagt
     2941 tgggatcgta ccaaccggaa cattaggcag tttttcatgg gacaaattaa taccagatac
     3001 tacgactgca tcctcatttg tttattttgc gccgatcatg tttattttta ctggtatcga
     3061 aatgtcctct gtgtatatca ctcgattgga aaatccagtt aaaacgtata ttcgcggagt
     3121 atttgccgcg ttaattttta tatttttcgt caatattttg aatgctttag ttgtagcaaa
     3181 tgtcgtacca aaaggacaaa tggaattgaa taatatcgcg caatctattt cgatttattg
     3241 ccaaattctt ggtttgccac atcttattgt gaatctgttt agtttacttg tcttcattgg
     3301 tgtagcagtc caattatctg cttgggcctc aggaccagca aagacagtga cagaaagtgc
     3361 tagaaaaggt gcgtatccac caaaattcaa tttttggaag acgaatcaat ttgacgtatc
     3421 caaatccgtt attttgactc agtctgtcat tatctcaatc tttgcacttt tttatttgct
     3481 gattcctggc gtcaatcaag catttttgat gctcgtaaat tcaactactg ttatctactg
     3541 tatcgtctat gtcatcatgg ggatcgcagt tttgagattg cgttatacac atgcaaagtt
     3601 gaatcgacca tttagaattg gaaaaaaaga gaaaaagagc aatcttggag tttggactgt
     3661 cgttattgtt ttatttgcgg caatcggatt ttctgtcgga ctgacgatga aagctggaac
     3721 atggatcaat ctaattgcag tcacagccat cagtgtcatt ttgtttgtag taccactctg
     3781 tattgaaaaa ataaagaaac cttcttggga acaggaagtc gccatgctgc ttgaaaaaga
     3841 aaaaggtgaa cttgatggaa aaaccggcaa tgaaacggac tctaactaat cgaaatatcc
     3901 agttaatcgc attaggtggc gcaattggaa caggattatt tttaggttca gcaaaggcaa
     3961 ttgcatcagc agggccagct gttattctcg tttatttgat atgcggtttg gctatttatg
     4021 gtttgatgcg ggccatgggt gatttatttt tagcaaatag tacatttaac tctatcagtg
     4081 atttcgttga ctattattta ggcaaaaagt gggcattttt tgtcaattgg acttattggc
     4141 tttgctggct tggagctgga cttgcggaat taacagcaat aggattgtat atccatttct
     4201 ggtttgtaca tgtaccagct ttgataagcg gaggtagcgc actaatgatc ttaatggcag
     4261 tcaaccttgt tgcagtcaag tggtttggtg aattggaatc ttttttctca atcatcaaga
     4321 tcttggggat tttccttttt atcttattgg gtataggatt gagtgtctca gattttcata
     4381 cccagtctat cggaatcaaa gaaaattttg gacagatcga tgaattacta ccgtttggct
     4441 ttgctggaat ggtagtctca tttcctatgg ttttattcag ttttgctgga attgaaatga
     4501 tcggcttaac cgttggcgag acaagtgatc cgcaaaagaa tttgaaaaaa gcaatcaact
     4561 gtctgccttg gcgtattctc tttttctata taggaacgat tttgtcgatt ttatttgttg
     4621 ttccatggca ttgtttagat ttaaaagaaa gtcctttagt gactatgctg caagtgatcc
     4681 attgccaagg cggagcagca attgtcaatc taatgatttt ggttgcttct ttgtcctcag
     4741 caaatagtgc agtctacagt actagcagga ttttatatca aacagcaaaa gaaaaaagtt
     4801 tgccccattg gttttgtgcg ctttctaaga aaggcgtacc tttacacggt attttaatta
     4861 caggaatgtt attttttgcc ggtctttgtc tacatttttt tgtagctgat agtcgattgt
     4921 tttttcagct tttggcaggc atgacaacga gttgttttct atttgtgtgg tcttcgatct
     4981 tgtgtgcaca tgttcaattt attaaagaaa agaaacggaa cactacacaa agatataaag
     5041 atcttgcttt gttggtgttt tttacttcaa ttgctttcag cttgttattc gaaagcacga
     5101 cacgctttat tcctttttta ttggttatct ggtttgtatt cttaaatttt gtatataaaa
     5161 gaataagcaa tagaaatatc agttaggccc agatacattt tgcaaaacga attatttcag
     5221 acccccctga aataatcgat acgcctgaaa aaacttccct gcgtataata acagtatcaa
     5281 ttaaacagga ggggatagga tgaactggta cagattgacg gtagaagatg tattgaaaaa
     5341 ctatcaaaca tcaacagaag gcttgtcgca agaagaacga cagaaacgcc tgactgatca
     5401 aggatataat gaaataaaag caaggcagca gacaaaacga tggaaaaaaa tagcaaagca
     5461 ttttactgat ctgctgatga tcgtgttgat cattgcctct tttttgaaat ttgcttctag
     5521 tgaatatatt gaaggaagta ttattttatt cgtagtgatc gtcaatggac tcgttagtta
     5581 ctggcaagaa agaaaagcgg aagaatcatt gaatggtttg aagcagctga tgggacaaga
     5641 agcgatagta ctatcaaacg ggatgcaaga aaaaatccct gcgaaagaac tggtaaaagg
     5701 agatgtcatt gtcttacgtg ccggagatgt cgttccagca gatatccgat taatcgaagc
     5761 acatgattta ttagtggaag aatcgatttt gacgggagaa tcagaagcat ctgaaaaaag
     5821 tcatgccgtt ttgacagaag aagaatcgat cggcgaccag aaaaatatgg gtttttcagg
     5881 aacgcttgta caatctggtt ctgcagtcgg tgtcgttgta gaaacaggaa acacgacaga
     5941 aatcggtaaa atcaatcaag cattgcaatc ggtcgaacaa caaacgacgc ctctgattaa
     6001 aaaaatcaac cgattaaaca aacaaatttt tcaaggcatt ctttgtttga gcctattctt
     6061 agttattttt acgacatttc gatatggtat ggattggcat atccttcttt cggctacgat
     6121 tgctttggtg gtgtcaatgg tacccgaagg attgccagct gttttgacca tgattctatc
     6181 cgttggagta catgaaatgg caaaagaaaa ggcgatcatc aaaggacttc cttcggtaga
     6241 aacattaggt tctatgacag tgatctgttc agataagaca ggaacattga caaaaaatga
     6301 aatgacagtt gtggatgtag ctgcaaaaga agaagcctgc gtgttgtcca ttatgaaaaa
     6361 ctgtcaggaa ttaaaaacaa aagaagaaca gaaaacagaa aatttaagtg gcaatccgac
     6421 agaagttgcg ctgattcgac acacagaaac agccaatcta cctttgaaaa aaatagaagc
     6481 aaagattccg ttcagttctg aatacaaata tatggcaacg atgcatgcgg aggaaaaagg
     6541 agcggtgatt tacgtcaaag gtgcaccaga agtattattt gctaaatcaa ctctttcttc
     6601 tgcagaacaa gaagcatgga gtcagacagc tgcagaattt gcgagaaaag gtcagcgtgt
     6661 tttaggattt gcttataaaa aagtagattc gaaacaggaa ctcacgcatg agactttaac
     6721 ccaattaact tttgcaggga tcgccggatt gatcgatccg ccaaaagaaa gtgcagtcaa
     6781 agctgtaaag gaatgccaac aagcagggat ctcagtgaaa atgattacag gggatcacaa
     6841 agacacagca aaagcgattg cggaacaaat tggcttaaaa catactgcca aagttttaga
     6901 agggatcgat ttggatctta tgtctgatga agagttgatt cagcaagtgc caatagtcga
     6961 tgtctttgct cgaacgacac cagaacataa actgcgaatt gttaaagctt tgcagaaaaa
     7021 tggagaaatc gtcggaatga ccggagacgg cgtaaatgat gcacctgctt tgaaaagatc
     7081 cgatgtagga attgctatgg gaatcaaagg tagtgaagta agcaaacagg cagctgatat
     7141 ggttttagga gatgataatt tccatacgat tgcaaaagcc gtaaaagagg gccggcggat
     7201 tttggataat ttgcaaaaaa ccatcaattt cttcttgcca actgcattag ctcaaggatt
     7261 gatcttgatt tgggcactta tgctgaatcg cccgctacct ttgtccccag tacagatttt
     7321 atgggtcaat atggtaacga caatcacttt atcttatgca ctagggttcg agtcgccaga
     7381 tccagaaatc atgaagcgcc caccaagaga tccaaagcaa ggaatcctat ctggttatca
     7441 tttgtttcgg atcatctatg tctccctact gatcatggtt cctgcctatt tattggctat
     7501 gcagtttgaa gggcaaagct tacagcaaac cattcttctg caaaatatcg tcttagctca
     7561 agctgtttac atgatcaatt gcagagagtt gtccaagccg tcattgaacc aaggtctttt
     7621 gcaaaacaaa gcgctttttc tttcattagg tatccttgct ttattgcaag ggatggttat
     7681 ttttctacca gtcggccagc agctgatcgg tacagtaagt ttaacgatga cacaacagtt
     7741 attgattgga gcaaatgtga ttcttctatt cttagttgta gaaatagaaa aagggatcat
     7801 gaacaaactg tttcgcaaaa aagaaaaaac agctagagaa tacagtcgct agccgttact
     7861 ataaaaaagt tttgaaagtg agttcagtca aacgattgag ctcatttttc gttggaatag
     7921 aagaaatgta tacaataggg atatctagag aatctagagg gaaattggaa atcaagatat
     7981 ctgttttttc aaaagaaata gtggaaagat cggttagttc gataggtaag aagcagacac
     8041 gttttcctaa gtaatctttt aattcctgct gcaagaacgc cttccatgaa ggttctcctt
     8101 ggaaaataaa atagacattg aacaaattcg gtttgataga aaaaatcgtt tgttggatca
     8161 gtagagacaa gagttggaga aagtaatttg gttcattgat ttctatttct ttttttagat
     8221 cgttcagtaa attctctttc tgttgataca agtgtgcata ttgctgatca tatgtttcta
     8281 aaaaacgatg gtattgacgg tccaatgctt caatcaatat cggcgattca tgatatttta
     8341 atatgaggag agccaagtta tcagtcagag atgttttttt tagttgatca agagacagct
     8401 ctgaagctag ttcctctaca aacgtttcga ggacctctaa aaatggagaa tgatgcaggc
     8461 agcgagcggt ttcttgtcca tagttattgt tgtaattcca actttccaga aagcagaaag
     8521 aagcaaaaga aagctcagtg tctttaagat aaataccttc cgttaaatac aattttttca
     8581 atgcagagtc atacagggag gagagtgtgc tgttggtatg gatagaagaa tctaatcggc
     8641 aattcgcttt tatacggatc gtattgataa agaaccaaca tgttccgaaa atctcttctg
     8701 tttcaaccgc tagttcagtt tgactgaggt ttcttaaaaa ttgaaaataa tgggattcat
     8761 ggatcgaata atcctcgaaa aaatagttgt tatgagtgaa aggaatcagc aatcgatgat
     8821 aaaaagtacg aatcgcacgc tcttttccaa gaatacaagc tgtatatttc gtcaaatcga
     8881 tctgcaagtc gtaacttgct aaacgctgat tgattttagt cacatgtctt ttcaatgtcc
     8941 cataagaaag cccatggtcg gctagaaaat cagggacagc aacttttttt gtaaaaagca
     9001 gatttttcag cagttcgaca ctgactgatt gtttgaaaaa aatctcccaa aggtcatttg
     9061 ttaataaatt ttgctggttt tgcaaactga ttcctgcttg atcgctgatg atgtcccagc
     9121 cttcgggcag ctgagagcga atagattgaa ggtcagaaaa aatcgttcgt tcggttgtcc
     9181 ctatgatttc agctaattca tttacggtta tctttgtatg attcagcaat tgttctacta
     9241 aagaaacatg acgtcgaata tctttttcgg taatgatttt tttcattagt gaatacatca
     9301 atcagactcc cttctattct aagaatacgg agaaaataaa cggtggtcaa gtttcaactg
     9361 atttttcaat cattgtttat ttttctggag catttactga tgataagaat ttcatcatga
     9421 acttgaaaaa tgataccact aaaatcaaaa aataggtggg caaacaataa taaacatttt
     9481 aatagtagat ggtagcaaga ccaacacagg cggattgaac atttaagagt tgataaaaaa
     9541 gaaaaatatc gaagtatttg attagcaact acttgtattc atttttctat tcttacgtgg
     9601 tatctttgaa taaagcgaat caataagaaa gagatggaag cagatgctaa cgaaaaaaga
     9661 actaatcgat cacatcaatg aaacatacaa tttgtctcca gaatatattt ttaaaaaatt
     9721 tcctgattat tgcgtattca gacatcaaaa aagcaaaaaa tggtttgggg tcctgatgtc
     9781 tgttccagaa gaaaaaatct atgatgatgc aagtgaccat aggatcatca atattcttga
     9841 tttgaaggtc gatcctgaac ttggcggaat tttgcgagga aaagaggggt attttccagc
     9901 ctatcacatg aataaagaac attggatctc tattgatcta cgtaaaatag aaaagctaga
     9961 tgaactagca gatttgattg atggaagttt taaatcaacg caatgaatgt ctaggacaat
    10021 agagaagagg ttgtgacaga aatgtttagc ttcaagaaat aagaaggaat ttacgaaaat
    10081 tgcttctcaa atttttggga aatttcggct tattttctaa gaaattactt ctggaacacc
    10141 gtttgttcga aaaaagagct gcgacaagaa ttttgtccca gctctttttt ttttattttc
    10201 ctaaataaaa ttcccgcatc gtttttgcaa tcattttgat caaaggatga tcttctgtga
    10261 agctagaaga taaataaatt gttcgtgtca agggcaaatc tttgatttcc cgcaagacga
    10321 aaggatcatt tttatcgtaa gaccaagaaa cttttgggaa aaagctaaca gctaatcctt
    10381 gcttgacaat agaacgtaat gtcgcaggat catctgtcgt cgaactgatc gtcaattcga
    10441 tacttttcgc ttcaagtgct gcatccagtg tatgacggaa ggcgttgctc tttgacagca
    10501 tcaagatggg ggtgcgttgg atatcttcta attgcagatg ttctttttta gcaagaggat
    10561 gtttcttagg aatggcgaca taaacaggtt ctttcatgat aggaatattg gtcatgccag
    10621 gcttcatctc cgaagtgatg tacagtacag gatcggtaat atcatctggt aaatgatgtt
    10681 gcatgatcgt taattggata tcaggatgct tttccataaa aattcggacc aagttcggaa
    10741 tagaaacaga ggctacctct aaatacaata tgacggaagt aacgatcttc tcattgaccg
    10801 aagacaataa tttgattcct tcttctaatg tgtctaatgt cttgactgca tatggataaa
    10861 attcttctgc ataaacggtc gtctcaatgt ttctgccgct atggtaaaac aaagggacac
    10921 ctaattcgtt ttccaaatgt ttgatcgcac ggctcaaggc aggttgagaa ataaataatt
    10981 ctttggctgc ttgggtaaca ttttttgttt cgaatacttt tttaaaatac tgcagatgaa
    11041 gcaatttcat aattatccat cccatccata cctttttgtt atggtttttt ctttttttgg
    11101 cattttttat tatagcagaa attgactaag atagcagtgg aaaaagtaag tgctgaggag
    11161 gcgaggctca tgagtacagg tttcttattt cctggacaag gcagtcagaa gttagggatg
    11221 ttagcggata tccctgaaat gtacatggaa cgtttagaaa atgtgacggg gtatcggtta
    11281 gttgataaag aagaaaacta tcaagatacg gtttttatcc agctagcatt actgacaaaa
    11341 gcagtatttt atttagatga aatggaaaaa aacggtattc agcctgatat tgcagcagga
    11401 cattcgattg gtgctttttg tgcagctgtt gcttgtcgat ctttgagatt tgagaatgca
    11461 gcggaactag tctatcaccg cgcagtctta atgaaagaag cttatccaag cggttatgca
    11521 atgggcgtag tagtaggtgt gacgagaagc gaagctgagg aaattgtagc aaaaacattt
    11581 gatcctgacc atccagttta tctgtcaaat gaaaattgtc cgatgcaaca tacgttatct
    11641 gggagtatcg aaggtttatc cgttacgttg gcagaagcca aacggcacca agcgcaaaca
    11701 gcgaagctgc tgaaagtccc tgtgccatct cactgtctct tgatgaatga tacggtcaag
    11761 aaattcaagc cgtatatgca gtctgtccat ctatctgatg ctagatgttt gtatataaaa
    11821 aatacggatg gaagagccac tcaggacgca gaagagattc gtaaagattt gttggaaaac
    11881 ctagctttcc ctgtgcagtg gaaccaaatg atggacttgg caaaagaatt aggaatggat
    11941 ctgacgattg aatttccccc cggcaatact ttgacaaagc tgattcatac aaaatttggt
    12001 gtagataaag ggatccgtac gatcaatcta gaccaacatg gtattgatga cgccacgttt
    12061 ctatatcaaa aatggagatg actaattatg gaaaaaccaa accgcaactg ggcttctaaa
    12121 cgcgaaaaaa aagcgcagcg attggaaaaa atcagcaact tgctcaatgg caagtatgta
    12181 gaaactgaaa aaatcgtaga agtaatggaa aaactgattg ctcccggcga caaagttgta
    12241 ttagaaggcg acaatcaaaa acaagcaagt tttctttcag aaagtcttga aaaagtagat
    12301 gcagaaaaag tgaatgatct gcatttgatc atgtctagta tctcacgacc agaacatttg
    12361 aatatttttg aaaaaggaat cgcaagcaag atcgatttct cttatgctgg tgcacaaagc
    12421 ttgcgtgtcg cccaaatgat tgaagacggc acaatgaaat taggtgatat ccatacttat
    12481 ttggaacttt atggacgctt attcatcgat ttgattccaa atgttgtttt agtagctgca
    12541 gataaagcag ataaagacgg caacttgtac acaggtttca atacggaaga aacaccaaca
    12601 atcatcgaag ctgcagcttt caaagatggt atcgtgatcg ttcaagttaa cgaagttgtc
    12661 gattctttac cacgtgtaga tatccctggt gactgggtag acgttgtcgt gaaagcagat
    12721 aaaccttatc aattagaagc tttattcaca cgagatcctc aaaacatcac agaattgcaa
    12781 attctaatgg caatgatggc aatcaaagga atctacgcag aacacggcgt acaatctttg
    12841 aatcacggta ttggattcaa tacagcagct atcgaattgt tactgccaac ttatggtgaa
    12901 tcactagggt taaaagggaa aatcgcgaag aactgggtat tgaacccaca tccaacgatg
    12961 attccagcca tcgaatcagg ttgggttgaa agcatccata gttttggtgg cgaagttggt
    13021 atggaaaaat atattgctgc tcgcccagat gtcttttttg tcggtaaaga cggcacgatg
    13081 cgcagtaacc gaacattatc acaagctgca ggacaatatg ccatcgatat gtttatcggt
    13141 tcaaccttgc aattagatta cgaaggaaac tcttctaccg taacaaaagg acgtttatct
    13201 ggttttggcg gtgcgcctaa catgggacat aatcctggcg gtcgtcgtca ttctacacca
    13261 gcatggcaat cacttcgcga caatgatgat ccattaggga aaggccaaaa attagttgtt
    13321 caaatggttg aaacatatgg ttcaaataaa aaaccagtct ttgtcgaaga attagatgca
    13381 ctagctgttc aaaaacaagc aggcttagcc aatgcaatgc accagtgatg atttatagcg
    13441 aagatactac acatatcgtg acagaagaag gaattgctta tttgtacaaa gcaaaaacaa
    13501 aagaagaacg acaagcagca attgcagcaa ttggcggtgt tacacctctt ggaatgacta
    13561 gcacgaaaga atcactagat agcttgcgtg cagccggcat cgtgaaatta ccagaagatc
    13621 taggcatcaa acgtagtgaa gcgaaacgtt cattattagc tgcacaaacc atcgatgatt
    13681 tgatcgaatg gtcagatggc ctgtatgaac caccaatgaa attcagaact tggtcataaa
    13741 attggcgaga aaaacctttt gtcgccattt acccagatgg aagaagaaag acgaataagc
    13801 agaacgtgta tgctctgttt tacaaaaagg ggagagatcc atggaacaat tagcttataa
    13861 atatccaacg aaacagccga tcaataagaa aatccatgta ggagtggtcg gttcaggaga
    13921 tttggaaatc ttgatgtttc caacagaaaa aacagaatct actgtaaaaa tttgtacagg
    13981 aagcgatggc ttcggcgaag tatggaacaa tgtattaacg agattttttg atcgttatcc
    14041 aatttcagca gatatcgtga tcaatgactt cggtgctaca ccaggagttg tgtacctacg
    14101 attgacgcaa gcaatggagg aactttcaga tgaaaaatag ttttgtagaa ttaaacgcca
    14161 gagaacgagt gaacgctcta cttgacaatg gacgtgaatt gattgatcca ttcgatcaaa
    14221 tcatcgcgcc aagtttacca gcacaaggaa tcgtccctga aagtgatgac gggatcgttg
    14281 tcagccgtgg cacgatcaat ggcaaacaag cagtagtaat cgctatggaa ggaaaattcc
    14341 aaggtggcgg tatcggtgaa gtttccggtg caaaaatcgt tgctgcactt gaacatgtgt
    14401 tagaagaaaa taaaaaagga aatgagatct ttccagtcat tgtactagat acaggtggtg
    14461 ttcgtttgca agaagcaaac tacggactgt tatctatttc agaaatcgga aatgtaatca
    14521 ttgctctaaa acaatatgta ccagtcatcg gtttgattcc aggacgtgta ggatcatttg
    14581 gcggtatgtc gattacttca gcattaatga gttatttaat cgcaacaaaa aaagcgagag
    14641 tcggtttgaa tggaccagaa gttattgaac aagaagcagg cgttcgagaa tttgattcta
    14701 gtgacaaaga cttgatttgg aatacattag gatctcgtca acgggtagaa tcaaaaatca
    14761 ttgatgaatt agtgactgat agtgtagaag caatcaaagc tgctgtcatc gcagcaatgg
    14821 acgaacgaaa agacacgcat cgcagtgaaa gctcagcatt ttatttatca ttacttgatt
    14881 ctttagatct ttcaaaacca atggccattg acgattacaa tgaagcaatt gctaaccatc
    14941 aagcaaaatc aattgactta ccagaagtag cagaaggaaa tgaaccagct gcagcaagtg
    15001 ttggttacga ttggtttgcg gcactaactg gcatgaaaaa tccaacttct tctattagta
    15061 ctgtgtatta tggtaaatca agtaaattca tagaagaagc catcgtgaca tcaatcgtac
    15121 ctgatcctaa aaacccgatg taccgtgtac gaaacggtga agttggttta gttgaaggtt
    15181 tccgcatggg acaaattttg gatcacgttt acgaagaaga taaagacaaa aaagtgaaac
    15241 ggccaatcat cgttgtgatc gatgtaccaa gccaagcata cggctataac gaagaattga
    15301 tcggtatcca tgtggctttg gctaatagtg ctgctgctta tgcaaaactc cgtcaagctg
    15361 gacatccagt tattggattg attgcaggaa atgctatttc aggtgcattt ttagcacatg
    15421 gattacaatc aagccgcttg attgctttaa acaataaaga aattaccgtc caagcgatgt
    15481 ctaaagaaag tgctgcacgt gtgacaaaac gttcggttgc tgaaatcgaa gcagctgctg
    15541 aaaaagttcc agctatcgct tacgatatca ataattatac aaaactagga gcgatctatc
    15601 atttcctaga agatatcacc gaccaagctg cttcagaaca aaatgtcagc aaagtcatcg
    15661 aagcaatcaa tgaagccatt caagatgtcc gcaatacaaa tgacacgatg ctgacaaatc
    15721 gatacacaaa cgaaacagcc aaaacattcg gacgtgtcga aacaaataaa gtccgtgcca
    15781 aaatggacga agaatgggat gcataaaaaa tgattcaagc acatgatatt gtcctttttg
    15841 ataaacatga aatagaaggg ttcaccttgc ctgaatgggt cgttctaact gaaaagatgt
    15901 tcggaacagt tcgtcgtgcg tcgacggatc acgcagaaat actaccgatt ggtttacgtg
    15961 gacctgcccg caatcagaga tggtcaacag aaatctcaaa acatgccgtc aaacaagtca
    16021 ttcatccgtg ggacatcatc gaacaagaat cgtatagaca agagaaaatc atggattatc
    16081 ctgtttatca gcaatttcat gctgctagaa acatattatc aggatgtaaa tggggaatcg
    16141 gcggcagctt aggatttgaa ttatcgactg ggattccggc agtaaaagaa accagtgatt
    16201 ttgatttgct gctgtacgca aattcaccga ttgaattgcc aattcaggta attcaatcgc
    16261 atcctgcttt ttttgagcaa ttcgataccc aagtagtgac tcggcaaggc ggtttttcac
    16321 taaaagagta tttgagagag ccagagaaaa aaattctatt gaaaacagtc gaaggaccta
    16381 aattaaccaa agaactttgg taataaagaa aaagagagag aaagagatgt ttgaaaacaa
    16441 aaaaatcaaa atcaacgtaa gtgacttcca gaaagtatca gctgtaacgg ctgttatgat
    16501 gcaaacgata ttgagctttg ctttggcaaa cagccacagc gcaacaaata cagcttttct
    16561 tggtaccttt tacgtcatcg ttaaatacac agcaccaatg tttatttttg caattgttta
    16621 taatatggta aaaacaagtc agcatttgtc ttattgggaa tttttgaaag aaaagttttt
    16681 tgaactagtc gttccgtatc ttctatggac gacggcttat ctttggctat ttccagcagt
    16741 ccaacaacaa actccgtata cggatactgc aagcttttta ctgaaatatg tcacaggtga
    16801 tggagcgcct catttatggt ataccgttat gatgctgcag atccaattgt tcatgccgtt
    16861 ttttgtttgg ctcggttaca aagtatttgc taataaaaaa tatgtatggc cagtattgat
    16921 tggttcaacg attttatatg tcgcatggta tctgttctat caaacacaag tattgactgg
    16981 gccttatcat gacagctggt acttattaga tcggtttgtt gctagtttca tgatttatgg
    17041 ggtagccgca tttgtttatc acgaaaaagt ctatcaatat ttggacagag tacgttatct
    17101 tttcttacct gttgccttag ttatcgcgtt cttttctgtc cgttcattgc tcgcacatcc
    17161 aggagacttg agctttgcga atgcacctta cttgaatacg atccaaagtt tatacagttt
    17221 agtcatcatc tttgctgtct tcataggcgc ttccaaaatg attgtaaacg attcgccaaa
    17281 attgccattg ttcaaatggt tgtctgttta tgcttatcgt acgtatttag ccaatgtatt
    17341 tgttttccaa gtactgttat tattgtttaa agatagttgg ctgcagttac cttcaggagt
    17401 catgattctt gtagcttatc taatgacggc aagttgtgca tttgctttaa gctggttact
    17461 tcatatcatt tgggtagcta tcaaaaaagg tttcagcaag taatagtagg aagaaggagt
    17521 gagtgtcatg ataaatgacg aacgaaaaaa agcagtgact cagcaactcg ctgcgttggt
    17581 agaggaagca ttgatcgcag aagtaacatt aagtccaaaa cctggattag tggatgcact
    17641 ggatgcgggt gcacattccg atatggacta tgctttattt cttaagtctg ctaagacgct
    17701 aactcctttt tttgaagaaa tggctcaaat agcatggcat catgctattg atcaggagtt
    17761 aagagaaaga attgctgaga ttgggcgaaa agcggaaaag gcaatgctga ttgctacaaa
    17821 tggcatcaat acccataaag gagcgatttg ggcaatgggg ctattgatca gtgtcattgc
    17881 acaaaaaatc agtatggaac agcctttttc tttatcgaaa atacttgatg atgtcagcaa
    17941 gcttgctgct tttcctgata caaaatatcg tcataaaaaa accactcatg gtcatagggt
    18001 gaagcaaaaa tatggcgtaa atggtgcata tgaagaagcg atattagggt atccgcatat
    18061 caggcttgca attcaagcag ctaaggagct agataatgat tcatatgtac agaaacaaat
    18121 gcatatgttg ttagttttga tgagttcgct agatgacaca tgcgtattgc atcgcagtga
    18181 tcagaaaacg ttagtggaaa tgcaaaaact tgctcatgct gctagtcaaa gtgctttacc
    18241 aaatcgtcca tttacagcac tgttgcactt ttgtcaggaa aaccaaatct ctcctggagg
    18301 aagcgcagat ctcttgtctg caagcttgtt catgctgaag atagaggaat tatggactgc
    18361 cgacacatac atttgtccga tgttactcaa tgactaaata agacaaaatt gctaaaaaaa
    18421 cagtttatgc atctggttat tgactcttcc ttttagcaag gctatcataa aaatagtttt
    18481 ttgtaataaa agataacgag gaagacaatg aaaaaaatca attatttagc aatcattttg
    18541 ttagtgggcc tatgtttgtt tgctctgccg acccaaggat tcgctgtcgc gggtggtcat
    18601 ggtggaggta catctagcgg tcgaagttcg ggcagaggtt catttggtgg tcgttcatat
    18661 gggggcagtt attttggcag tcgttcttac ggcagagatt cttatgggta ttatggtcca
    18721 ggtacaggtc cttctagtta tgagcaaacg ggatttcttg cattagctgt tttgtcagta
    18781 ggaggggttt ctgctttttc tatcgtcaag aataaaagac aaaagaaaag gctgaaagcg
    18841 gagatattgt cccgcatgtc aggaacggct aaagaaaaga aagcacagtt tctggaaatc
    18901 caaaagatat tcatgaagat ccaatccgct tgggaagaag aaactcctga aaaagccaga
    18961 agctgttata cagacaagct gtttgccgaa catcagaaag ttctgcaaaa aaacaaagca
    19021 gacggtgtaa gaaatcatac taaaaaaata actattcagg aagtaggaaa ttatcgacag
    19081 atccacgaaa atagttttgc catccgaatt gatttcagat gctgtgatta ttcagtcgat
    19141 cgaaaagatg ggcgattgat ctcaggaagt aagacacaac gacagtattt ttctcagata
    19201 tggtattttg atttttctga gaaagaaaaa agatggcaag ccgattttat tcagccgatt
    19261 atgctggatt gacaggaaat atttatgaac ggagttgtga caaaagcctt gtcgcagctc
    19321 ctttttctga ataaacggtg tgccagagat agcctatcgg caataaggca cgattttgta
    19381 aaatatggga agcgattttt caaaatcgct tcttatcgct caaggctgaa catctctgtc
    19441 acaacctctc cactaaatgg tgtttaaaag cttgcatctg cgataataag aacaaccaaa
    19501 cgaaaaatag gaagcactat tttagtttag ttgttcttat tacttgatgc aggacagctt
    19561 tttcacaacc tcttttttgt atagatgggc aaaatttttg taataatagg cgtatgtctt
    19621 tttcgttgag gagatgaaaa agcatatggc ttattttttg tttgctttgc ttactttctt
    19681 tccacttcaa gtggcagaaa tatctacttt ttattatgta agcggaacca tatacttgaa
    19741 atcagaagga gatcatttct atgaaaacaa gaacaattga aatacttcac agaatcatga
    19801 cagcccaata tccagtaaaa gcaaaacatc tagcaagtga gttcaatgtc agtcagcgga
    19861 cgatccgtca ggaagttctt gaagcgaata tctggttgag cagtaaaaag cttccggaga
    19921 tccgtacaat tcgcaacaaa ggttttttgt tgaagctgga aaaggaaaag caagctcgat
    19981 tagaaactga aataaaagat gtgaaagacg gctttttgaa tcggcgcgaa cgtatttttg
    20041 atctagtttt agccgttgct tacagccgaa tcccgatcca tctaaacaaa aaagaagaag
    20101 aatttcaaat ctctaaaagt acattagatg aagatatgag acggttacga gcaacactgg
    20161 tcagttatgg gatagagatc gtcagttatg gaaagcaagg actggtttat aaaggttcag
    20221 aacgatctat ccgtacgatg atctatgatt ttatcaatca gaatctggga atgaccgact
    20281 tctttatgaa tcaagatgca caattaacac cggctcaaaa aatctttctc cattattttc
    20341 ctttggtgga aatcaaaaaa atacaggaga tctattccaa taagttgttg aaacaagaag
    20401 atgatattta taaaaaacaa atcctgcttt ttacattgat ctggatccag cgaatcagga
    20461 aacatgaatt tatctctgcc attaattgga aaaatacaga aataaaagac agtagctttt
    20521 ctgcatttat tgaccacatg atcgaggaat ttcaattgga caatgtcccg caagtagaaa
    20581 gaaattatct tcgtttcact attgagacgt tcaatgctag agatatctcc aattcaatcg
    20641 agtgggtaca agctcaatta ctaacgatcc aattgatcca atttgtagag aacgagacac
    20701 acgtaccttt ccatctaaag gaagaagctt tattcgaaaa tctctataag cacatggctg
    20761 cactgattgt aagaatcaag aatcatatcc aagtcatgaa tccattgaaa gaaaacatcc
    20821 gaacaaatta tttcccaatc taccgcgcag tcactcattt tgttcctacg atcgaagaag
    20881 taattggtgg caagatactg gaggacgaga tagcttttct tgtcatccac ttttcgacga
    20941 tcgccagtgt aatcaagcga gacttgaatt atgtttataa atcagtcgtt gtctgtaacc
    21001 atgggatggc tactggaaat ctgttagcag agaatctgaa agaaaagttt cctcagatcg
    21061 agattactgc agttttaagt tcaaaagaaa tcgaattagt agataaattg gatgtcaatc
    21121 tggttttcag cacctttcaa ttgaattatc gaaataagcc gcttttagtt gtcgatccga
    21181 ttttaactga gacgaatatc ttggttatag aagacttttt gaaaagaaat gaagaactac
    21241 agcggttaga gcctaacagt catgaatcga cggagttatt cactcaaata atagatgtca
    21301 tgaaagaaag tggtggccga gtagatcgaa tcatctatgg caagttagaa aaattattcg
    21361 aaacaaacaa tttagaaatc aacaaaaggg agatccagcc tatgttaaaa gatattttga
    21421 cggacagcaa tatcatgatc aaagaaaaag ccactacttg ggaggaatcg attgaactag
    21481 ctgctagacc attggttaaa gaaaacattg tcgaagagcg ctatgtcgaa gcgatgattc
    21541 gtgcagtgaa tgattacgga ccgtatatcg tgattggcaa acacatggca ctagcccatg
    21601 cccgaccaga agatggggtc aataaattag gagtcagcgt agctacgatt gagcaaccaa
    21661 tcgatttcgg taatcctgaa atggatccag taaagattat tttttgtttg gcagcagtag
    21721 actcctactc gcatctgaac atcatgaaag aactaattga attgatcaac aacgaagaga
    21781 agttggatcg attgattgaa tgcacaaata tcgaagattt caaacaactg cttttttcag
    21841 tagttgagaa ataataaatg atacggggga gaacaaagat gaacaaattg aacattttat
    21901 tcgtatgcgg tgcaggttta ggaagtagtt ttgcagcaca gatgtctgct gaggatgttt
    21961 tgaacaaatt aggagtagaa gccaaactgg accattgtga tatttcttcc gcagtttcca
    22021 tgaaaccaga tgtgatcatc acagcagaaa attttcgttc gcagttcgaa aagttcgcag
    22081 ttgatcctaa aacggcgatg atctatttga aaaatattgt ttcaaaaaat gaaattgaag
    22141 aaaaaatcac cccagttctg caagataagg gagttatcta aatacaaaaa gaaaagaggg
    22201 taaaa
//
LOCUS       DADVXN010000043        22021 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.044, whole genome shotgun sequence.
ACCESSION   DADVXN010000043 DADVXN010000000
VERSION     DADVXN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.044"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="KB942_001401"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="KB942_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBB6851835.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="KB942_001402"
     CDS             complement(2020..2922)
                     /locus_tag="KB942_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6851836.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="KB942_001403"
     CDS             complement(2919..3611)
                     /locus_tag="KB942_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBB6851837.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="KB942_001404"
     CDS             complement(3654..4295)
                     /locus_tag="KB942_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6851838.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="KB942_001405"
     CDS             complement(4333..4647)
                     /locus_tag="KB942_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851839.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="KB942_001406"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="KB942_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="KB942_001407"
     CDS             complement(5984..6916)
                     /locus_tag="KB942_001407"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBB6851840.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="KB942_001408"
     CDS             complement(6913..7689)
                     /locus_tag="KB942_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBB6851841.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB942_001409"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB942_001409"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBB6851842.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB942_001410"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB942_001410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBB6851843.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="KB942_001411"
     CDS             complement(12014..13294)
                     /locus_tag="KB942_001411"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBB6851844.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="KB942_001412"
     CDS             complement(13313..14239)
                     /locus_tag="KB942_001412"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBB6851845.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="KB942_001413"
     CDS             complement(14252..15535)
                     /locus_tag="KB942_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBB6851846.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB942_001414"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB942_001414"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBB6851847.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="KB942_001415"
     CDS             complement(16323..17234)
                     /locus_tag="KB942_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6851848.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="KB942_001416"
     CDS             complement(17231..17695)
                     /locus_tag="KB942_001416"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBB6851849.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="KB942_001417"
     CDS             complement(17700..18398)
                     /locus_tag="KB942_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6851850.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="KB942_001418"
     CDS             complement(18385..18771)
                     /locus_tag="KB942_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851851.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="KB942_001419"
     CDS             complement(18918..20588)
                     /locus_tag="KB942_001419"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBB6851852.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="KB942_001420"
     CDS             20771..21004
                     /locus_tag="KB942_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBB6851853.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="KB942_001421"
     CDS             complement(21103..21534)
                     /locus_tag="KB942_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBB6851854.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>22021
                     /locus_tag="KB942_001422"
     CDS             21613..>22021
                     /locus_tag="KB942_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBB6851855.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKT"
BASE COUNT         6228 a         4924 c         3889 g         6980 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 c
//
LOCUS       DADVXN010000044        21165 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.045, whole genome shotgun sequence.
ACCESSION   DADVXN010000044 DADVXN010000000
VERSION     DADVXN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.045"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            41..1702
                     /locus_tag="KB942_001423"
     CDS             41..1702
                     /locus_tag="KB942_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6851856.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1772..2134)
                     /locus_tag="KB942_001424"
     CDS             complement(1772..2134)
                     /locus_tag="KB942_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBB6851857.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2202..3143
                     /locus_tag="KB942_001425"
     CDS             2202..3143
                     /locus_tag="KB942_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851858.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3144..4187
                     /locus_tag="KB942_001426"
     CDS             3144..4187
                     /locus_tag="KB942_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851859.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4193..5263
                     /locus_tag="KB942_001427"
     CDS             4193..5263
                     /locus_tag="KB942_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851860.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5253..6206
                     /locus_tag="KB942_001428"
     CDS             5253..6206
                     /locus_tag="KB942_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6851861.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6218..7228
                     /locus_tag="KB942_001429"
     CDS             6218..7228
                     /locus_tag="KB942_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6851862.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7513..8334)
                     /locus_tag="KB942_001430"
     CDS             complement(7513..8334)
                     /locus_tag="KB942_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6851863.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8431..8709)
                     /locus_tag="KB942_001431"
     CDS             complement(8431..8709)
                     /locus_tag="KB942_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851864.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8977..10986
                     /gene="metG"
                     /locus_tag="KB942_001432"
     CDS             8977..10986
                     /gene="metG"
                     /locus_tag="KB942_001432"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBB6851865.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11465..12052)
                     /locus_tag="KB942_001433"
     CDS             complement(11465..12052)
                     /locus_tag="KB942_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851866.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12227..13549
                     /locus_tag="KB942_001434"
     CDS             12227..13549
                     /locus_tag="KB942_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6851867.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13704..14480
                     /locus_tag="KB942_001435"
     CDS             13704..14480
                     /locus_tag="KB942_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6851868.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14473..15042
                     /gene="rnmV"
                     /locus_tag="KB942_001436"
     CDS             14473..15042
                     /gene="rnmV"
                     /locus_tag="KB942_001436"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBB6851869.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15035..15919
                     /gene="rsmA"
                     /locus_tag="KB942_001437"
     CDS             15035..15919
                     /gene="rsmA"
                     /locus_tag="KB942_001437"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBB6851870.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16370..16999
                     /locus_tag="KB942_001438"
     CDS             16370..16999
                     /locus_tag="KB942_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851871.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="KB942_001439"
     CDS             complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="KB942_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBB6851872.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18441..18863
                     /gene="mgsA"
                     /locus_tag="KB942_001440"
     CDS             18441..18863
                     /gene="mgsA"
                     /locus_tag="KB942_001440"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBB6851873.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19186..19710
                     /locus_tag="KB942_001441"
     CDS             19186..19710
                     /locus_tag="KB942_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6851874.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKIWLFVK"
     gene            complement(19760..21061)
                     /locus_tag="KB942_001442"
     CDS             complement(19760..21061)
                     /locus_tag="KB942_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6851875.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         6817 a         3818 c         4337 g         6193 t
ORIGIN      
        1 tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt
       61 tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac
      121 aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt
      181 gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc
      241 attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc
      301 aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg
      361 agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg
      421 gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga
      481 aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt
      541 tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct
      601 tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta
      661 tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg
      721 accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac
      781 agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt
      841 attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc
      901 aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca
      961 acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat
     1021 cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt
     1081 gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg
     1141 aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga
     1201 attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa
     1261 agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc
     1321 aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt
     1381 actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac
     1441 aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc
     1501 agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat
     1561 caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct
     1621 agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa
     1681 atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg
     1741 gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa
     1801 tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc
     1861 tggcagacga gagcgtggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat
     1921 ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga
     1981 taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg
     2041 aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg
     2101 ggatagattt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
     2161 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
     2221 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
     2281 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
     2341 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
     2401 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
     2461 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
     2521 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
     2581 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
     2641 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
     2701 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
     2761 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
     2821 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
     2881 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
     2941 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
     3001 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
     3061 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
     3121 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
     3181 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
     3241 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
     3301 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
     3361 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
     3421 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
     3481 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
     3541 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
     3601 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
     3661 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
     3721 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
     3781 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
     3841 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
     3901 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
     3961 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
     4021 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
     4081 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
     4141 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
     4201 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
     4261 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
     4321 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
     4381 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
     4441 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
     4501 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
     4561 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
     4621 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
     4681 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
     4741 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
     4801 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
     4861 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
     4921 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
     4981 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
     5041 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
     5101 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
     5161 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
     5221 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
     5281 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
     5341 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
     5401 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
     5461 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
     5521 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
     5581 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
     5641 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
     5701 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
     5761 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
     5821 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
     5881 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
     5941 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
     6001 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
     6061 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
     6121 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
     6181 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
     6241 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
     6301 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
     6361 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
     6421 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
     6481 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
     6541 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
     6601 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
     6661 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
     6721 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
     6781 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
     6841 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
     6901 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
     6961 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
     7021 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
     7081 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
     7141 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
     7201 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
     7261 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
     7321 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
     7381 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
     7441 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
     7501 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
     7561 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
     7621 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
     7681 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
     7741 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
     7801 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
     7861 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
     7921 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
     7981 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
     8041 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
     8101 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
     8161 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
     8221 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
     8281 tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
     8341 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
     8401 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
     8461 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
     8521 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
     8581 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
     8641 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
     8701 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
     8761 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
     8821 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
     8881 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
     8941 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
     9001 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
     9061 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
     9121 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
     9181 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
     9241 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
     9301 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
     9361 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
     9421 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
     9481 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
     9541 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
     9601 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
     9661 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
     9721 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
     9781 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
     9841 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
     9901 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
     9961 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    10021 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    10081 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    10141 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    10201 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    10261 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    10321 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    10381 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    10441 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    10501 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    10561 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    10621 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    10681 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    10741 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    10801 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    10861 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    10921 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    10981 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    11041 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    11101 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga tttttgttaa
    11161 tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga taaagggtcg
    11221 ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt cataaagttt
    11281 gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga tgtgttaatt
    11341 ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat
    11401 agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat
    11461 cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag
    11521 cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat
    11581 aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc
    11641 cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag
    11701 gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga
    11761 tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca
    11821 aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg
    11881 cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa
    11941 ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga
    12001 cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt
    12061 cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct
    12121 taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag
    12181 ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact
    12241 aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc
    12301 aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt
    12361 gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt
    12421 agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa
    12481 attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc
    12541 tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta
    12601 tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac
    12661 aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt
    12721 gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga
    12781 agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt
    12841 tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa
    12901 agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat
    12961 caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc
    13021 ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat
    13081 cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta
    13141 tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa
    13201 gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag
    13261 ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt
    13321 actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata
    13381 taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt
    13441 aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat
    13501 aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac
    13561 aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata
    13621 aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt
    13681 aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta aatgctgaac
    13741 agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa
    13801 tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact
    13861 atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag
    13921 aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa
    13981 ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca
    14041 gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac
    14101 ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc
    14161 atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt
    14221 catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg
    14281 taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta
    14341 gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc
    14401 ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg
    14461 gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac
    14521 gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca
    14581 atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt
    14641 tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct
    14701 caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt
    14761 ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca
    14821 gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta
    14881 atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt
    14941 tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa
    15001 ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac
    15061 accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg
    15121 acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga
    15181 tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc
    15241 caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc
    15301 tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga
    15361 tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa
    15421 tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt
    15481 ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg
    15541 aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc
    15601 attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa
    15661 actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt
    15721 gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta
    15781 tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga
    15841 tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat
    15901 gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc
    15961 atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct
    16021 tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt
    16081 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
    16141 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
    16201 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
    16261 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
    16321 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
    16381 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
    16441 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
    16501 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
    16561 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
    16621 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
    16681 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
    16741 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
    16801 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
    16861 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
    16921 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
    16981 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
    17041 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    17101 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    17161 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    17221 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    17281 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    17341 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    17401 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    17461 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    17521 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    17581 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    17641 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    17701 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    17761 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    17821 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    17881 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    17941 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    18001 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    18061 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    18121 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    18181 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    18241 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    18301 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    18361 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    18421 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    18481 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    18541 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    18601 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    18661 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    18721 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    18781 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    18841 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    18901 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    18961 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    19021 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    19081 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    19141 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    19201 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    19261 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    19321 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    19381 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    19441 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    19501 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    19561 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    19621 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    19681 tacaaaaaaa tttggctctt tgtcaaatag gactgataga gtgagataag aacatctaag
    19741 gtagaattcc ttttctactt tagatgtttt ttgattcata ggtaatcaga ttatttagta
    19801 agaaaattct ggtcttcatt ttccgaaagc ttcgaaagcc ataagcgact cgtttcaatg
    19861 ctttgatgtg ggtatgcaga ttttctattt tggcatttga ataaggcaat tcgagggcta
    19921 aacgaatgcc ggattcatag tttataaaag ctttttttat agaatgcagt tcttttggaa
    19981 cggaattggg catcgttctt acaagtttaa agaaagtgtc cgcctctttg tcgtgaaacg
    20041 cttgaagaat ttcttgatag taaaaatagg cgattcgtaa ttcgtcagag atcgaaagca
    20101 gacgatccac cacttcactt tcggtgagat acgcccaacc aaaattgcgc catttggtcc
    20161 atttgtcata aacaagcttg tcctgtcgtt tgatcagaag ccgagacaac gcctttaatt
    20221 gtcgataata tttggcttgt gttggatcgt ttcgatcaaa gcttttcatg attcgtacgc
    20281 gaatatcctc aaatttacga atgagatgtt tgacaatgtg aaaacgatcc acaacgagtt
    20341 ttgcctttgg aaaacattcc tttatcagag aggcataaga aacattcatg tccgtcacga
    20401 tgacctttac cttcaatcgt gcgcgtcgtt caaattgcat aaaataagaa acgagagcct
    20461 ttttcttccg tgaaggaaga atatccagta attttccagt atcgccatcc atacaagaaa
    20521 aggagtaggg accgacttga tttttcagtg aacgaaattc atccattaat aaacaacgcg
    20581 gtaaccaatt tctttttact tttgtcatgg ggatcagaga agcgagtgtc cgagaaacgg
    20641 tcgaccaaga aacgccgcaa tgcttagcaa ttgtcgtcat tgcttgtttt tcagttagct
    20701 cttctaaaat tttatagcgc acttgtttag cgataaagca gtttctatcg acaatgtagg
    20761 tattcgccgt aaaataggtg tggcagccac gacagtggta gcgttgtttc gctaaagcaa
    20821 gataagtcag ttggttcgca catttattaa gttgaatgag cgattttttc tttccgttct
    20881 taacgatcat gccttcatga ttcatctgtt tacacaaagg acagcgacga atttctttac
    20941 ttaacgtcgc agaaacaaca agactcttga tattcttaat gctcttcgat tccacatttg
    21001 tgatgaaaat gtttttatct ttgattccaa gtagtagttt gatatgatga agatcaggca
    21061 tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct tctgtatgtt
    21121 ttgttttttc atgctcaaaa gggaccgata gaatgatttt ctatc
//
LOCUS       DADVXN010000045        21068 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.046, whole genome shotgun sequence.
ACCESSION   DADVXN010000045 DADVXN010000000
VERSION     DADVXN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.046"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(42..230)
                     /locus_tag="KB942_001443"
     CDS             complement(42..230)
                     /locus_tag="KB942_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBB6851876.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="KB942_001444"
     CDS             500..862
                     /locus_tag="KB942_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6851877.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="KB942_001445"
     CDS             914..2596
                     /locus_tag="KB942_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBB6851878.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="KB942_001446"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="KB942_001446"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBB6851879.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="KB942_001447"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="KB942_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBB6851880.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="KB942_001448"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="KB942_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6851881.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="KB942_001449"
     CDS             6431..7435
                     /locus_tag="KB942_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851882.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="KB942_001450"
     CDS             7436..8380
                     /locus_tag="KB942_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851883.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="KB942_001451"
     CDS             8380..9342
                     /locus_tag="KB942_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851884.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="KB942_001452"
     CDS             9369..10283
                     /locus_tag="KB942_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851885.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="KB942_001453"
     CDS             10309..12090
                     /locus_tag="KB942_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6851886.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="KB942_001454"
     CDS             12438..13124
                     /locus_tag="KB942_001454"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBB6851887.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="KB942_001455"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="KB942_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBB6851888.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="KB942_001456"
     CDS             16734..17543
                     /locus_tag="KB942_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6851889.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="KB942_001457"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="KB942_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBB6851890.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="KB942_001458"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="KB942_001458"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBB6851891.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         7150 a         3749 c         4667 g         5502 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgca
//
LOCUS       DADVXN010000046        20143 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.048, whole genome shotgun sequence.
ACCESSION   DADVXN010000046 DADVXN010000000
VERSION     DADVXN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.048"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            176..988
                     /locus_tag="KB942_001459"
     CDS             176..988
                     /locus_tag="KB942_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBB6851892.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            985..1785
                     /locus_tag="KB942_001460"
     CDS             985..1785
                     /locus_tag="KB942_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBB6851893.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1946..3214
                     /gene="rseP"
                     /locus_tag="KB942_001461"
     CDS             1946..3214
                     /gene="rseP"
                     /locus_tag="KB942_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBB6851894.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3282..4991
                     /locus_tag="KB942_001462"
     CDS             3282..4991
                     /locus_tag="KB942_001462"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBB6851895.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5203..9561
                     /locus_tag="KB942_001463"
     CDS             5203..9561
                     /locus_tag="KB942_001463"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBB6851896.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9706..10179
                     /gene="rimP"
                     /locus_tag="KB942_001464"
     CDS             9706..10179
                     /gene="rimP"
                     /locus_tag="KB942_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBB6851897.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10202..11377
                     /gene="nusA"
                     /locus_tag="KB942_001465"
     CDS             10202..11377
                     /gene="nusA"
                     /locus_tag="KB942_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBB6851898.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11399..11692
                     /locus_tag="KB942_001466"
     CDS             11399..11692
                     /locus_tag="KB942_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBB6851899.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11689..12000
                     /locus_tag="KB942_001467"
     CDS             11689..12000
                     /locus_tag="KB942_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBB6851900.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            12013..14319
                     /gene="infB"
                     /locus_tag="KB942_001468"
     CDS             12013..14319
                     /gene="infB"
                     /locus_tag="KB942_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBB6851901.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14346..14693
                     /gene="rbfA"
                     /locus_tag="KB942_001469"
     CDS             14346..14693
                     /gene="rbfA"
                     /locus_tag="KB942_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBB6851902.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14850..15023)
                     /locus_tag="KB942_001470"
     CDS             complement(14850..15023)
                     /locus_tag="KB942_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6851903.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15471..17833
                     /locus_tag="KB942_001471"
                     /pseudo
     CDS             15471..17833
                     /locus_tag="KB942_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18201..19124
                     /gene="truB"
                     /locus_tag="KB942_001472"
     CDS             18201..19124
                     /gene="truB"
                     /locus_tag="KB942_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBB6851904.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19128..20069
                     /gene="ribF"
                     /locus_tag="KB942_001473"
     CDS             19128..20069
                     /gene="ribF"
                     /locus_tag="KB942_001473"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBB6851905.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6912 a         3534 c         4408 g         5289 t
ORIGIN      
        1 gggcggatca ttccgcttcc tctgttttta tttatatttc ttttatttaa aaattaaaaa
       61 aagaaaaaat tattcagttt ttaaataaaa aaacaatgat ttcataggaa aaacagttag
      121 atattgctat aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt
      181 acgttttttt ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac
      241 cgatggacca attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca
      301 aaaccgtcgt ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt
      361 cacaaaaaaa gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga
      421 aaactggaag cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt
      481 tgatacgttc gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga
      541 aaacgtattg ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa
      601 caatactgga atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac
      661 tgctgtccaa gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga
      721 tgatgaattg attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc
      781 tgagctgatg atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat
      841 cgcatatagt gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct
      901 tgaagaagca atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac
      961 agaagatgag aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta
     1021 gtcttgttta ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc
     1081 ttagcagtaa taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt
     1141 gaaggagttt tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt
     1201 ttctttcttc cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt
     1261 ttaggaacat cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg
     1321 cttgtcagct tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc
     1381 ttggctgttt tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg
     1441 gttggtcgac gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt
     1501 gaaggtgcgc tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta
     1561 tacccagcaa aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt
     1621 ttttcagcag tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat
     1681 gtgaaagact cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt
     1741 ttgctgtttg tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt
     1801 ttctttgtga atttcataaa agacgcaggt ttttattttt cattatattg atactattgg
     1861 tgtctttggt atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa
     1921 aaaacaagta aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg
     1981 tatattagtg attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct
     2041 cgttcgcgaa tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg
     2101 gacaacatat acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg
     2161 ggatgatgaa acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg
     2221 catcgtagag aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga
     2281 attgagccgt tatgatttag aagatgagct gactatcact ggttatgtga atggagacga
     2341 aacagaagta gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac
     2401 agaaatcaga attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat
     2461 gctgaccaac tttgctggac ccatgaataa ctttattttg gcaatcctgt tattcatcat
     2521 tctggcattt atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat
     2581 gcctaatgga gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga
     2641 cggaaaagaa atccattcat ggaatgatct gacaacagtc attacgaaaa atccggataa
     2701 aacattagac tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa
     2761 atctgtagag tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac
     2821 aggttttatg gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat
     2881 tttcaaagcc ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt
     2941 catgatgtac caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt
     3001 gatggcgctt ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga
     3061 tggcgggaaa ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga
     3121 aaaagaagga atattgacat tagctggctt tggattttta atgttgttga tggtgctagt
     3181 tacatggaac gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata
     3241 acaaacgata actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt
     3301 tgattccaac attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc
     3361 taagagctgg atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa
     3421 atcgtgtatt ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg
     3481 tagaaatgct gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg
     3541 aaacatatgg accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg
     3601 gaccaactca cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga
     3661 aattaccttt aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct
     3721 tcggtttgct tcgcggaaga gagtttatca tgaaagatgc gtattctttc catgcaacgg
     3781 aagaaagttt ggacgagaca tataaagatt acgaaaaagc ctacaccgaa gtattcaagc
     3841 gttgtggctt agaatttcgc tcgattatcg gagacggtgg cgcaatgggc gggaaagact
     3901 caaaagaatt catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa
     3961 gtgattatgc tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg
     4021 aaacacagct tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag
     4081 cagatttctt ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg
     4141 aacaacctgt ccttgttttg gtacgcggag accatgaagt caatgacgta aaactgaaaa
     4201 actttttatc tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag
     4261 gtgctgagtt tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag
     4321 accgttatgt ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact
     4381 ataaaaatgt gaatccagaa cgcgatttca ctgtactttc ttatgaagat cttcgttttg
     4441 tacaagaagg agatccttca ccagacaaca atggtgttct tgcctttact cgaggaatcg
     4501 aaatcggaca tatcttcaaa atcggcactc gttacagtga atctatggga gcaactgtac
     4561 tagacgaaaa tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc
     4621 gtttattatc agcaatcgta gaacaacatt cagatgaaca aggaatcaac tggccaaaag
     4681 gaattgcgcc atttgatttg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa
     4741 acctgacgaa tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg
     4801 acagaaatga acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca
     4861 gaatcactgt cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg
     4921 gcgaaatggt tgaagtacgt aaagaagaat tggtgaatac tttaccaatc ctcttgaacc
     4981 aaggcgaata ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga
     5041 caatctgatg attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt
     5101 aatgtaaaac tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat
     5161 gcgatcaatt gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat
     5221 ttatttgaaa aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg
     5281 cttactgctg gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt
     5341 acgctgcatt tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg
     5401 actatcgctt ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca
     5461 ttcgatgaac agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat
     5521 acaccactag cacagcaagt attgaaaacg caggtacctg tgataaaaga tcgtaaagtc
     5581 atcttgccaa tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta
     5641 gtagaagagt tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat
     5701 gagcagcaag cggaacgagt attgaaactt tttgaagaac gcaaacaaga acaggcagaa
     5761 gcctttatga agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga
     5821 aaagagcaat cgccagctct tgaaggaccc atccacatcc agctaggacg aaatattcca
     5881 gccgatgaac caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt
     5941 gaggggtacg tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact
     6001 ttgaaaatca cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa
     6061 gatgaacaag tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt
     6121 caagaagata cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa
     6181 catacacctc gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat
     6241 agcaatatga gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcaggg
     6301 aaatggggac accgagctat cgccattaca gaccacggtg gtgcgcaagc ctttccggaa
     6361 gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc
     6421 gtagatgatg gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca
     6481 tatgtcgtat ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag
     6541 ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat
     6601 ccaggacatc ctttgtcacg aacaacggta gatctgactg gtatcacgga tggaatggtt
     6661 cgaggatcaa agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca
     6721 attttggtag cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg
     6781 tatggtattc ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg
     6841 tatcctcaat tcaaacgttt cggattaggt gtgttatcta aaaaatttgg tgtcagcttg
     6901 gaacagcatc accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt
     6961 gtgaaagagg caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc
     7021 ggagaaggag attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat
     7081 caggcaggac tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa
     7141 cgcgtcccac gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc
     7201 tcagcttgtg ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca
     7261 agaaaccgag caaaattcta cgattatatt gaagttatgc caaaagcggt ctatgcgcct
     7321 ttgatcgagc aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg
     7381 gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg
     7441 aacgaagaag atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg
     7501 ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca
     7561 tttcactttt taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc
     7621 gcagatatct gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca
     7681 ggttcagaag atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat
     7741 ccgttacctg agatcattca aaaacgatta aaaaaagagt tgaattctat taatggtaat
     7801 ggcttctcgg ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc
     7861 tatctagtag gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc
     7921 actgaagtta atccgttagc ccctcattat tattgtccag agtgccaata ttccgagttt
     7981 tttgaagatg gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt
     8041 ggtgcacgct taaacaaaga cggtcatgat attccatttg agacattttt aggattccat
     8101 ggagacaaag tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat
     8161 aactatacga aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca
     8221 gtcgccgaca aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag
     8281 tttcgaagtg cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc
     8341 ggccagcatc caggggggat catcgttatt cccgattata tggatgtcta tgattttacg
     8401 ccgatccaat atccagcaga cgatcaaaat tcggaatgga aaacgactca ttttgatttt
     8461 cactcgattc acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt
     8521 attcggatgc ttcaagattt atcagggatc gatccacaaa cgatccctac ggatgacccg
     8581 gaagtgatgc ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat
     8641 tccaaaacag ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgcta
     8701 gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt
     8761 acagacgtat ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg
     8821 gaagtaatcg gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc
     8881 gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat
     8941 gaattgcgag aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc
     9001 gattcttgtt caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg
     9061 atggctcttc gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac
     9121 ttttctgtac gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg
     9181 aaacaagcaa tgaaagaaat cacggacaaa ggattagatg cttccgtaaa agaaaagaat
     9241 caactgacag tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg
     9301 attgatttgt ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca
     9361 ccatttagag ctgtcccaag tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga
     9421 aaagacggtc catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca
     9481 ttgatcgaat atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta
     9541 tcgttattcg atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt
     9601 atggtatagt tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc
     9661 tttttaatgg aattacgcac ttaatttttt aaggaggcga aacttttgag tagcgtcgtt
     9721 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9781 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9841 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9901 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9961 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
    10021 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
    10081 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
    10141 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10201 aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa
    10261 agaaatcgtg atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg
    10321 ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc
    10381 ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc
    10441 acttttaatc aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa
    10501 agattttggg cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga
    10561 agcagaaaga acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg
    10621 aatcgtcgaa cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt
    10681 tctttctaaa caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt
    10741 ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca
    10801 tccagaccta ttgcgccgct tatttgagca agaagtacca gaagtctatg atggaatcgt
    10861 tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa
    10921 tgatccaaat atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc
    10981 aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc
    11041 tgtattcatt gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac
    11101 aaatccaaag gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa
    11161 acgcggacaa aacgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc
    11221 agaatctgac atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact
    11281 gcatgatgaa gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt
    11341 caacaatgaa acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat
    11401 gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa
    11461 agagatggtc cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa
    11521 aatgcctgga cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga
    11581 taaaaaaatt ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact
    11641 tcttgactat gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt
    11701 aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg
    11761 ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca
    11821 accgatgcaa gcgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt
    11881 ccttgctttg agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag
    11941 gtattcggaa taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag
    12001 gaaggtgatt gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct
    12061 agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt
    12121 gcgattacta gcaatgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa
    12181 caacaagctg cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt
    12241 aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc
    12301 ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg
    12361 ggaaatacca gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact
    12421 aacaataaca acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat
    12481 cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca
    12541 ccacgcaaat tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca
    12601 gatattgcga aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt
    12661 ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg
    12721 gactatggca tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc
    12781 ttcgaggcag aagaagtcga tccagataaa ttagtctcac gtccgccagt cgtaacgatt
    12841 atgggacacg tagaccatgg gaaaacaact ttattggata cattacgcca ttcacgtgta
    12901 acaagcggcg aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat
    12961 ggtaaaccaa tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca
    13021 cgtggagcaa gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg
    13081 ccacaaacag ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct
    13141 gtaaataaaa ttgataaacc aggtgcaaac cctcaacatg ttatgcaaga attaagtgag
    13201 tatgagttaa ttcctgaagc atggggtgga gaaacgatct ttgttgagat ctcagcgaaa
    13261 ttcggccaaa acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat
    13321 ttaaaagcag atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa
    13381 ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa cacttcatgt tggtgaccca
    13441 atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc
    13501 gataaagaag caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa
    13561 gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa
    13621 cgcgcgaaac gtgcattatt agaaaaccgt gctgccacaa gccgtgtgac tttggataac
    13681 ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat
    13741 gtacaaggtt cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc
    13801 cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca
    13861 gcagccagca acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg
    13921 caagctgata ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa
    13981 gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt
    14041 caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat
    14101 gtaacagaag gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta
    14161 atctatgaag gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa
    14221 atgggttatg aatgtggtgc aatgattgaa aaattcaatg acatcaaagt agatgacgta
    14281 attgaaggat ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa
    14341 tcattatggc taactaccgt gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg
    14401 atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga
    14461 aagttacggg ggacttacag caagcaacga tctattacag cattttatct gacttagcat
    14521 cagataagaa aacagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt
    14581 tagggcacaa tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag
    14641 ttgtctatgg caaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa
    14701 ctcccgcaaa tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat
    14761 tacctaagag ttccatgact ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa
    14821 gaaaagtaaa aaattcgact ttcttttttc tagtagtata taaatttttg catatagata
    14881 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14941 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    15001 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    15061 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    15121 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15181 gaattgctcg atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag
    15241 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15301 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15361 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15421 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15481 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15541 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg
    15601 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15661 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac
    15721 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15781 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg
    15841 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15901 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15961 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    16021 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    16081 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    16141 ttaacggact tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc
    16201 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag
    16261 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16321 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16381 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16441 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16501 aagaaatcag agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16561 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16621 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16681 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16741 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16801 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16861 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta
    16921 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa
    16981 atcacatgac ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac
    17041 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
    17101 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
    17161 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
    17221 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
    17281 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
    17341 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
    17401 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
    17461 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
    17521 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
    17581 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
    17641 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
    17701 caaaggaagt caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca
    17761 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
    17821 tcttgaccaa taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag
    17881 aaactgaaaa aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt
    17941 ttcgtttttg ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca
    18001 aatggtgcaa agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt
    18061 tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca
    18121 ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa
    18181 aaatgaaatg gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga
    18241 caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg
    18301 ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa
    18361 aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt
    18421 tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt
    18481 ctttatctga aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga
    18541 taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca
    18601 ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa
    18661 gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg
    18721 ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg
    18781 cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga
    18841 cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa
    18901 tagaaaaagg agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag
    18961 taaaaaacgg tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag
    19021 aaattgcttt gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa
    19081 aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca
    19141 agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg
    19201 gatttttcga cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg
    19261 cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct
    19321 ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa
    19381 aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc
    19441 atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg
    19501 tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg
    19561 aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa
    19621 aaatcagctc tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa
    19681 aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca
    19741 gactactagg attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag
    19801 aaggtgttta tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa
    19861 ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg
    19921 actttaatca agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag
    19981 atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag
    20041 caacagctga atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta
    20101 taactagcaa ttacaataaa atgggtgata aataagccca gga
//
LOCUS       DADVXN010000047        19633 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.049, whole genome shotgun sequence.
ACCESSION   DADVXN010000047 DADVXN010000000
VERSION     DADVXN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.049"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB942_001474"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB942_001474"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBB6851906.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="KB942_001475"
     CDS             complement(1172..1867)
                     /locus_tag="KB942_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBB6851907.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="KB942_001476"
     CDS             complement(1880..2596)
                     /locus_tag="KB942_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBB6851908.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="KB942_001477"
     CDS             2926..3534
                     /locus_tag="KB942_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBB6851909.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="KB942_001478"
     CDS             3859..4182
                     /locus_tag="KB942_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6851910.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="KB942_001479"
     CDS             4364..5734
                     /locus_tag="KB942_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBB6851911.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="KB942_001480"
     CDS             complement(5864..7165)
                     /locus_tag="KB942_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6851912.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="KB942_001481"
     CDS             complement(7519..9477)
                     /locus_tag="KB942_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBB6851913.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="KB942_001482"
     CDS             complement(9654..9920)
                     /locus_tag="KB942_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6851914.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB942_001483"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB942_001483"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBB6851915.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB942_001484"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB942_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBB6851916.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="KB942_001485"
     CDS             13264..13827
                     /locus_tag="KB942_001485"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6851917.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="KB942_001486"
     CDS             complement(14080..14601)
                     /locus_tag="KB942_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBB6851918.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="KB942_001487"
     CDS             complement(14604..15446)
                     /locus_tag="KB942_001487"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBB6851919.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="KB942_001488"
     CDS             complement(15439..16164)
                     /locus_tag="KB942_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBB6851920.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="KB942_001489"
     CDS             16340..16636
                     /locus_tag="KB942_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851921.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="KB942_001490"
     CDS             17204..18796
                     /locus_tag="KB942_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6851922.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="KB942_001491"
     CDS             18809..19549
                     /locus_tag="KB942_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6851923.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5619 a         4028 c         3725 g         6261 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaa
//
LOCUS       DADVXN010000048        19398 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.050, whole genome shotgun sequence.
ACCESSION   DADVXN010000048 DADVXN010000000
VERSION     DADVXN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.050"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            352..1041
                     /locus_tag="KB942_001492"
     CDS             352..1041
                     /locus_tag="KB942_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6851924.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="KB942_001493"
     CDS             1028..2236
                     /locus_tag="KB942_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6851925.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="KB942_001494"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="KB942_001494"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBB6851926.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="KB942_001495"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="KB942_001495"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBB6851927.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="KB942_001496"
     CDS             complement(6339..7034)
                     /locus_tag="KB942_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBB6851928.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="KB942_001497"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="KB942_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBB6851929.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="KB942_001498"
     CDS             complement(8233..8424)
                     /locus_tag="KB942_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBB6851930.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="KB942_001499"
     CDS             complement(8445..9335)
                     /locus_tag="KB942_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBB6851931.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="KB942_001500"
     CDS             complement(9322..10089)
                     /locus_tag="KB942_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6851932.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="KB942_001501"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="KB942_001501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBB6851933.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="KB942_001502"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="KB942_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBB6851934.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="KB942_001503"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="KB942_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBB6851935.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="KB942_001504"
     CDS             14660..15043
                     /locus_tag="KB942_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851936.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="KB942_001505"
     CDS             complement(15211..15969)
                     /locus_tag="KB942_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBB6851937.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="KB942_001506"
     CDS             complement(15987..16805)
                     /locus_tag="KB942_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6851938.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="KB942_001507"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="KB942_001507"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBB6851939.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="KB942_001508"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="KB942_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBB6851940.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="KB942_001509"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="KB942_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBB6851941.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5501 a         3988 c         3572 g         6337 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacat
//
LOCUS       DADVXN010000049        19176 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.051, whole genome shotgun sequence.
ACCESSION   DADVXN010000049 DADVXN010000000
VERSION     DADVXN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.051"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            176..1192
                     /locus_tag="KB942_001510"
                     /pseudo
     CDS             176..1192
                     /locus_tag="KB942_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1209..1358
                     /locus_tag="KB942_001511"
     CDS             1209..1358
                     /locus_tag="KB942_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851942.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            1355..1801
                     /locus_tag="KB942_001512"
     CDS             1355..1801
                     /locus_tag="KB942_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851943.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1803..1940
                     /locus_tag="KB942_001513"
     CDS             1803..1940
                     /locus_tag="KB942_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6851944.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            1977..2270
                     /locus_tag="KB942_001514"
     CDS             1977..2270
                     /locus_tag="KB942_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851945.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            2293..2490
                     /locus_tag="KB942_001515"
     CDS             2293..2490
                     /locus_tag="KB942_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6851946.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            2501..3520
                     /locus_tag="KB942_001516"
     CDS             2501..3520
                     /locus_tag="KB942_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6851947.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            3777..4424
                     /locus_tag="KB942_001517"
     CDS             3777..4424
                     /locus_tag="KB942_001517"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6851948.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(4749..4895)
                     /locus_tag="KB942_001518"
                     /pseudo
     CDS             complement(4749..4895)
                     /locus_tag="KB942_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            5134..5826
                     /locus_tag="KB942_001519"
     CDS             5134..5826
                     /locus_tag="KB942_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6851949.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(6108..6180)
                     /locus_tag="KB942_001520"
     tRNA            complement(6108..6180)
                     /locus_tag="KB942_001520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6145..6147),aa:Lys,seq:ctt)
     gene            6561..7130
                     /locus_tag="KB942_001521"
     CDS             6561..7130
                     /locus_tag="KB942_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBB6851950.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7117..7977
                     /locus_tag="KB942_001522"
     CDS             7117..7977
                     /locus_tag="KB942_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6851951.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8167..8871
                     /locus_tag="KB942_001523"
     CDS             8167..8871
                     /locus_tag="KB942_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6851952.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8876..10711
                     /gene="walK"
                     /locus_tag="KB942_001524"
     CDS             8876..10711
                     /gene="walK"
                     /locus_tag="KB942_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBB6851953.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10708..12024
                     /locus_tag="KB942_001525"
     CDS             10708..12024
                     /locus_tag="KB942_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851954.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12025..12894
                     /locus_tag="KB942_001526"
     CDS             12025..12894
                     /locus_tag="KB942_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBB6851955.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12949..13758
                     /locus_tag="KB942_001527"
     CDS             12949..13758
                     /locus_tag="KB942_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6851956.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13861..14490)
                     /locus_tag="KB942_001528"
     CDS             complement(13861..14490)
                     /locus_tag="KB942_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBB6851957.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14660..15952
                     /locus_tag="KB942_001529"
     CDS             14660..15952
                     /locus_tag="KB942_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6851958.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(16028..16690)
                     /locus_tag="KB942_001530"
     CDS             complement(16028..16690)
                     /locus_tag="KB942_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6851959.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16915..17199
                     /gene="groES"
                     /locus_tag="KB942_001531"
     CDS             16915..17199
                     /gene="groES"
                     /locus_tag="KB942_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBB6851960.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17250..18875
                     /gene="groL"
                     /locus_tag="KB942_001532"
     CDS             17250..18875
                     /gene="groL"
                     /locus_tag="KB942_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBB6851961.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6612 a         3187 c         3999 g         5378 t
ORIGIN      
        1 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
       61 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
      121 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
      181 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
      241 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
      301 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
      361 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
      421 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
      481 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
      541 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
      601 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
      661 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
      721 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
      781 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
      841 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
      901 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
      961 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
     1021 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
     1081 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
     1141 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
     1201 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
     1261 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
     1321 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
     1381 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
     1441 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
     1501 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
     1561 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
     1621 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
     1681 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
     1741 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
     1801 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
     1861 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
     1921 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
     1981 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
     2041 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
     2101 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
     2161 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
     2221 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
     2281 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
     2341 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
     2401 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
     2461 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
     2521 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
     2581 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
     2641 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
     2701 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
     2761 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
     2821 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
     2881 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
     2941 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
     3001 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
     3061 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
     3121 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
     3181 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
     3241 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
     3301 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
     3361 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
     3421 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
     3481 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc tcgtttttga
     3541 ggggaagtac ataatgcaaa tttgtccata acttattgat tatcttgtaa aaatgcggta
     3601 atttaatagt tagtgagaag aggtgcttaa ttatagttaa aaaagaggaa atagaagcag
     3661 catgtaacca agcaaatgtt aggtttattg gagaagcttt atctggatat cctaagtata
     3721 aatacttaca caactggtta caaagtaaaa gtttttatct taataaagaa agatgcatgc
     3781 ctaataaaaa ttatagggtt tataaaagag gaactattgt atatgttgat tttggggtga
     3841 acgtgggtta tgagttatct ggtaatcatt ttgctattgt tttaaataat aaagataatt
     3901 cgaaaaatgg tgttgtatgt gtagtaccaa tctcttcaaa aaagaaaagt aattatgtat
     3961 ctgtaggaaa aatattagaa gttgctagta ttaaacaatt tataagccaa gctgataaat
     4021 taaaagagaa acttaggata attattttac tttcattaaa tagaggcatc attgatgaaa
     4081 gcgatttgaa tagttttatt aaagatattg aagctaaagg tgaaaagctt tcagtggaag
     4141 aagtagaaaa acgagctgta aaacataatt tggtttgcga gacaagggaa caagtaatta
     4201 aagaaataaa atcattatct gaagatatgg taaaatatca gaaggtgttt aattgctaca
     4261 agaaatatac acaagaatcc tatgtaatgc cacttaatat ccaaacgatt agtaaaaata
     4321 gaattagaag aattaacaag tttgatcctt ctggaaatat gaaggcacct ggaaatgtcc
     4381 tcaatgaagt agatgaaagt ttaaaaaaat attttacaaa gtaaacgata tttttgttga
     4441 catttttctg ttcgttagtt accatgtagt tatagaagga cgtaagcgcc tatttttaat
     4501 gtacacatcg ggacgtatgc gcccacatag aaacatagtt gagagacatc tatatggtgt
     4561 ctcttttttt attaatatgc aaaaaagtct agttttttca ataatattta tgtaataata
     4621 aattagccac cacaacaaag aatgaaatcc attattatct agtctagtcc attctttttg
     4681 tttgcagtat ttgtgatggc tttccgtacc cttagctcag ttggtcagag cagacggctc
     4741 ataaccgtcc ggtcgtactt ctcgtaatcc aggaaggccg tcagctccgt ccgcagtagc
     4801 gtgtttacgg ccagctccag atgggagcgg aataactcgt tcaaatcacc tttggtggct
     4861 agagtctgca ggagttctgt aataaaatcg ttcataggga agtcctcttt tctgtgaatg
     4921 ggtgtggtaa ctttattcta cagaagggac ttcctttttt ctatttacac aaaatatttt
     4981 atactctcaa atgtttgtat gcagttacat ctgactttat tcgaattaag gaaaagattt
     5041 ataaaaataa aataaaaaaa tatatattta aattgactta ttgtgaaatc attaactata
     5101 attagttcag tttatgttaa ggagaatgat gatatgaata agaaaattgt agtaagtgga
     5161 ttaattatag gtggattagt gttagcaggg atcgcaggga aaaactttgc cgatgacatt
     5221 ttgttatggg gtggagaaga taatattgaa caaatcaatc ataatttgga gtcattagat
     5281 aaagcactag aaaataaaga gcaaaatatt agtagtttaa attcacgatt atcttcaaat
     5341 aatcaagaac ttgagcaagc aaaggctaac gttgaagaat ataaacaaaa agtgttgact
     5401 ttggaaaatg ataaaaatca attaactata gagaaaacaa atttagagaa tcaattatca
     5461 ggaaaaaatg ctgaattgca aggtaaacaa aatgaaatca atgctaaaat tgatgaaata
     5521 aatcaaaaaa tagctgaaat aaatcaatta aacactgatt ggaataatcg tttacaaaca
     5581 gaacaaaata aatttaatga tgctcaaaat actgttaata gtttaaatca acaaattaat
     5641 caattgtcaa atgaaaaaaa tactattaaa aaccaattgc aaagtactga acaaaaacta
     5701 gaaaaagcgc aatcagaaaa cagtaatttg aataattata ttgaaaaact ggaaaaagcc
     5761 aaaagcgatg tagaagacac agcaaacaga tcacaagaaa tcgtgactga acatacagga
     5821 aaatagatgc attttttagt ttaaatttct ttaacaaaat agtattttgt tgctaaataa
     5881 tttttatttg aatattctac aatgatatct cccctcaaaa tggaggggaa gtacatactg
     5941 tgataatcta caggtttatt gactgattag gatgttatta aaaaataaaa tactaaaatg
     6001 catacccgaa tttcataccc gaaaaataaa aaatgccacc acgtacactc ttttcgaaac
     6061 tacctaagta gccaagaatg tactggtgac gtgggtttgg cacccaatgg gccgtgaggg
     6121 gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc
     6181 gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa
     6241 agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg
     6301 ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac
     6361 ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat
     6421 aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat
     6481 cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa
     6541 tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg
     6601 aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg
     6661 accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc
     6721 cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag
     6781 ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac
     6841 aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa
     6901 ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc
     6961 aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata
     7021 ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg
     7081 ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt
     7141 ttagtagatt caggtacgga tgtgccagaa gaattgataa aaaaatacga gatgtatatg
     7201 atcccattga aaattattta taaagatcag gtatatacag ataaaattga cattacctct
     7261 gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag
     7321 tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta
     7381 acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa
     7441 gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct
     7501 gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat
     7561 ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt
     7621 caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac
     7681 ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt
     7741 aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc
     7801 ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga
     7861 cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta
     7921 gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc
     7981 aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa
     8041 aaaataataa agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga
     8101 tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg
     8161 ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa
     8221 ttcaatttag ccaaagaagg ctatgatgtt tatactgcat atgatgggga agaagccttg
     8281 gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg
     8341 gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta
     8401 acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac
     8461 gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc
     8521 ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat
     8581 ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca
     8641 catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga
     8701 gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat
     8761 gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta
     8821 gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa
     8881 aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat
     8941 tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac
     9001 gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag
     9061 cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag
     9121 atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg
     9181 gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta
     9241 tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg
     9301 ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct
     9361 ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt
     9421 ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg
     9481 ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga
     9541 ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt
     9601 caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg
     9661 tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa
     9721 agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat
     9781 cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt
     9841 agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat
     9901 gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt
     9961 agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt
    10021 ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc
    10081 attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct
    10141 agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga
    10201 tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt
    10261 tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga
    10321 atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga
    10381 taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt
    10441 attagaaacg cataataacg tggttttaag tatcaccgat caaggattgg ggattcctaa
    10501 aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc
    10561 acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg
    10621 cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata
    10681 tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga
    10741 ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca
    10801 ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat
    10861 cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc
    10921 caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga
    10981 tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat
    11041 ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa
    11101 gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt
    11161 actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat
    11221 ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca
    11281 aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct
    11341 acgaaacatt cgatcaaact gaaaaagtat agttatatcc tgtcacagca accctataca
    11401 ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat
    11461 gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt
    11521 gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc
    11581 ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac
    11641 aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga
    11701 ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa
    11761 accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt
    11821 acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca
    11881 gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta
    11941 cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa
    12001 ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc
    12061 gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa
    12121 aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat
    12181 atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag
    12241 cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg
    12301 gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct
    12361 gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac
    12421 aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa
    12481 gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat
    12541 acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac
    12601 atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa
    12661 gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta
    12721 tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct
    12781 gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag tgtcaacgct
    12841 ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc
    12901 gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa
    12961 aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc
    13021 gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc
    13081 ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa
    13141 catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt
    13201 gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa
    13261 aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc
    13321 ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca
    13381 ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat
    13441 gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat
    13501 ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt
    13561 gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt
    13621 agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa
    13681 aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact
    13741 gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc
    13801 aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta
    13861 ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac
    13921 taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ccagcagcgt ttctttgtaa
    13981 ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc
    14041 tggactgata taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta
    14101 catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt
    14161 cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc
    14221 ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc
    14281 tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa
    14341 ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac
    14401 gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg
    14461 gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt
    14521 gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc
    14581 ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag
    14641 aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac
    14701 atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc
    14761 aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc
    14821 tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt
    14881 tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga
    14941 catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc
    15001 actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac
    15061 aactgggctc atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa
    15121 gcaaagagag aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat
    15181 tgttatagtc acttttacgc ttatcggttc agcggttttt caacagttgt tcagaatgac
    15241 tgttgttcaa ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt
    15301 gtttggtgtg cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg
    15361 atctgcgatg gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcgaatgc
    15421 agaaatctta gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca
    15481 gcaatttctc gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt
    15541 ttttatgacg gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt
    15601 accagccttt ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc
    15661 gatgatggct gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc
    15721 tttatatttt ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc
    15781 gatcctctcg ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt
    15841 gtcaattagt tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa
    15901 aaaggaaaga cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa
    15961 aatactcctg tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata
    16021 gtcatgttta tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca
    16081 ttcctacgtg tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga
    16141 agaagaaacc taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca
    16201 gcaaagagct gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta
    16261 tactgcggcg gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa
    16321 aagcgggtgc ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt
    16381 tgccaaacaa tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga
    16441 ttccaaccaa tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga
    16501 tcgctaaggg ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat
    16561 acatgactgc agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta
    16621 ggatcaaggg tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt
    16681 ttaaagacat gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg
    16741 attgatttca attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt
    16801 tgatttaaaa gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac
    16861 tcgaaggtta agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta
    16921 aaaccattag gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga
    16981 ggaatcgttt tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc
    17041 ggtgaaggcc gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac
    17101 caagttatgt tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg
    17161 atcgtcgcag gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa
    17221 tcaaaaataa tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg
    17281 cacgcgcagc tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag
    17341 gacctaaagg ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg
    17401 atggtgtgac gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa
    17461 aacttgtatc agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag
    17521 caactgttct gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta
    17581 atcctctagg cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc
    17641 ataatatctc aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt
    17701 cgggttctga taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg
    17761 gtgtcatcac aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta
    17821 tgcagtttga tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag
    17881 ctgttctaga aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata
    17941 ttctgccatt gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg
    18001 atgtagatgg tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg
    18061 tagtagctgt aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg
    18121 ctattttgac aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgtaa
    18181 caatcgaaaa cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg
    18241 tagaaggttc tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa
    18301 ttgctgaaac aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag
    18361 caggtggtgt agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa
    18421 aattacgcat tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct
    18481 caggtggtgg tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag
    18541 gagacgtggc aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa
    18601 tcgctgaaaa cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac
    18661 taggcactgg tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg
    18721 tagacccaac taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt
    18781 tattaacgac agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg
    18841 caatggatcc atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa
    18901 ctgatcatta gtgaaagaca gataaaaaga ggctggaaca gaagcgttta actccaagaa
    18961 ataagaagaa attcacggaa attgcttttc aaatttttgt gaatttcagc ttatttccga
    19021 aggagttgct tctgcttccg ctgtttatac gtgtttagag cgtgagacaa aagtgttctt
    19081 tacttttgtc ccacgctctt ttttgtgtgt aattataatg aaaatagtta tttttaatag
    19141 ttataatatt ttatctatta aaagtaaata tccatt
//
LOCUS       DADVXN010000050        19101 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.052, whole genome shotgun sequence.
ACCESSION   DADVXN010000050 DADVXN010000000
VERSION     DADVXN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.052"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..586)
                     /locus_tag="KB942_001533"
     CDS             complement(<1..586)
                     /locus_tag="KB942_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6851962.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLL"
     gene            complement(588..1352)
                     /locus_tag="KB942_001534"
     CDS             complement(588..1352)
                     /locus_tag="KB942_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBB6851963.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1360..2037)
                     /locus_tag="KB942_001535"
     CDS             complement(1360..2037)
                     /locus_tag="KB942_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBB6851964.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2074..3459)
                     /locus_tag="KB942_001536"
     CDS             complement(2074..3459)
                     /locus_tag="KB942_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6851965.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3669..3753)
                     /gene="ffs"
                     /locus_tag="KB942_001537"
     ncRNA           complement(3669..3753)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KB942_001537"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3776..4291)
                     /gene="tadA"
                     /locus_tag="KB942_001538"
     CDS             complement(3776..4291)
                     /gene="tadA"
                     /locus_tag="KB942_001538"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBB6851966.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4397..5254
                     /locus_tag="KB942_001539"
     CDS             4397..5254
                     /locus_tag="KB942_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851967.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5335..5670)
                     /locus_tag="KB942_001540"
     CDS             complement(5335..5670)
                     /locus_tag="KB942_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851968.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5737..7107)
                     /locus_tag="KB942_001541"
     CDS             complement(5737..7107)
                     /locus_tag="KB942_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBB6851969.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7185..7721)
                     /locus_tag="KB942_001542"
     CDS             complement(7185..7721)
                     /locus_tag="KB942_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6851970.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8030..9511
                     /locus_tag="KB942_001543"
     CDS             8030..9511
                     /locus_tag="KB942_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6851971.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(9614..10321)
                     /locus_tag="KB942_001544"
     CDS             complement(9614..10321)
                     /locus_tag="KB942_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBB6851972.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(10400..10738)
                     /locus_tag="KB942_001545"
     CDS             complement(10400..10738)
                     /locus_tag="KB942_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBB6851973.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(10849..11811)
                     /locus_tag="KB942_001546"
     CDS             complement(10849..11811)
                     /locus_tag="KB942_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBB6851974.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(11826..13157)
                     /locus_tag="KB942_001547"
     CDS             complement(11826..13157)
                     /locus_tag="KB942_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6851975.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(13165..13845)
                     /locus_tag="KB942_001548"
     CDS             complement(13165..13845)
                     /locus_tag="KB942_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB6851976.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(14064..14543)
                     /locus_tag="KB942_001549"
     CDS             complement(14064..14543)
                     /locus_tag="KB942_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBB6851977.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(14670..16349)
                     /locus_tag="KB942_001550"
     CDS             complement(14670..16349)
                     /locus_tag="KB942_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6851978.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            16897..18399
                     /locus_tag="KB942_001551"
     CDS             16897..18399
                     /locus_tag="KB942_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6851979.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            18412..19074
                     /locus_tag="KB942_001552"
     CDS             18412..19074
                     /locus_tag="KB942_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6851980.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
BASE COUNT         5639 a         4017 c         3217 g         6228 t
ORIGIN      
        1 ctaataatac aaaaaataag ataaagctat gcatgaaaaa ttcttctgga agtacattga
       61 tgattggatc agtaacggga tcgaataacc agtcatcgtt gttgaaaaac gtttcatgaa
      121 aaaggataaa aaagcgatca aatcctatca tcatgaaaaa accgaaaaca actggcagta
      181 acatgccgat ttgaaacgga cgaatcaacc gccaaagccg atcattcttt tgcaaatatt
      241 tgataaacaa aatactcggg atcaaagtga tgaaaaacac agcataatcc actaaaaaca
      301 aaattttcac ttcataaaag tgatgggctc cgctagcaga aactgggaaa tctggcaagc
      361 tgaggatggt tttgaaggga ttattgagat aattcattag gtgatcaaaa tttttcagta
      421 acgtctcttg atctaaagaa gtataatcca aaatttgcaa atgtccgata tcaaatacgt
      481 atagtggtcg aaagttgatc gtcacaccaa ctgccagact gatcagtgtc agaaataggc
      541 tgaacaatcc agcatattcc atccactgcc atctcgaatt tttcatatta gaagctccat
      601 tcatccagtg attgtttcac ataggtagct gggataggca acccaggtac atcttcttct
      661 tttgtaaaac ctgataaaac gagcaaagta tctatattat tgcgtatccc agcttggata
      721 tctgtttcat agttatctcc aaccatgatt acttcttctt tttgcagccc taatacttca
      781 acggctttat ccataatgat ggcttctggt tttccgatgt agatcggcgg cgtttgggtt
      841 gctgtctcta caaaagagat gactgaccct gctccaggaa gaaggccgcg ttcagtaggt
      901 atattcttat ctgggttcgt tccaatgaaa gtagcacctt tttgaatagc aagtgttgct
      961 ttaactactt tttcataggt cagataatta tctaagccta ccactacata gtcaggagcc
     1021 tcttcttccc atgtaaatcc ggcagctaaa atcaagtcaa tcaatcctgc ttctccgata
     1081 acatagactt ttttcccttt tccatctgct ttcataaaat cgattgttgc caaagtagct
     1141 gtgtagactg tctctggagc tacatgaatg tcaaactcat ctgctagacg attagctact
     1201 gtttctggtg acttagtcgt gttattggta acaaacaaga aaggtaattt tcttttttgc
     1261 aattcttcca caaatcgttt accggccggt atgggttgtg tcccacgata aatcgttccg
     1321 tctaaatcaa ttaaatatcc tttatagtcc attcatccac tactcttccc tttttctgat
     1381 cgtaaatgct gtttttgttt tttctacttg tttaggttgt tcttgttttt ttactttttc
     1441 agaggttttt gctgactctg tttttcgatt tttaataacc ggttttttgc gattagatgc
     1501 gactggtcct tttttctcat cgatatacgc acgattatgg ttttttttct tttttcggcg
     1561 ctgagttgtc ttttcttttt taccgccgat tctctcgatg acaaaatacg cacaaccaaa
     1621 attgcaatat tcgtaaagat agtcttccaa ggaatcgatt cgctgatctg gatgcgcccg
     1681 tcgattatcc gcttggaaaa atccttttag gcgtagttgt tcataacccc aatctcctac
     1741 gatataatca tatcgggcta gtacatcact gtaacgttct ccaagctttt ctgcattaaa
     1801 tccttcacga taatcggaca caagccgata atgtctttca cctatcaaaa aattactgtc
     1861 atctaacagt gtcactagct cgcctttgac tttttctgct ttaggctcct caacgatttc
     1921 ttccaaagca gctgtcagtt cttcggtgac tgttgtttcc tgttcagctg atgatttttt
     1981 cttagcaatt tctgttttat gctcaacgtt ctgctgttca ttttgttttg cagacatgcg
     2041 ttcaccacct atctttttta ttataccttg tttttatttg attgggtagt gagctttcaa
     2101 gtaatctcct accacacttc ggataaattc agggaacaaa aattcatttt ttccagcaat
     2161 ctcaatcgtt ggaaaaaatg gaacaaacat caaatgatct acggtagtaa aagtataatt
     2221 tttattcaga tcgattggct tgttcttcca aaagacttga tggttaatag aatcatatgt
     2281 gattccatta tagaccatct gcccaaaaat cttaccgcga aatcccattc ccttcatagg
     2341 atagttacgt aaaaagttac gatttttttc tatttctaaa actaagcgga tcagatcatt
     2401 tccacccaat gtgacattta acaagtgcat cggatgcggc aacgcagtgt gaagctgatc
     2461 ctgattgacc cgcccttttg gaatttcagt taaaaacaga ccactgttta atattgctgc
     2521 ttccgtcttt cctcgttctt tcaccgcctc aaaggctact tggataaacg aatgctcatt
     2581 gaagatatct agactcagag aaaatggcaa atctgctact tctttttctt ttaataattc
     2641 atgcccacgt tctagacaac tatttatttc ttgttcatct tctgtaaaag ctgtcattgt
     2701 ttcggtaggg atggctttgg ctgaatgctt aaggattttt ttctcttcat cgatggttaa
     2761 atggacttca ccgacatact gcccgtactt tccagcggct gctatttgga catcattgat
     2821 tacgtgacct tctctgaata aatgatgggt gtgcgatcct aaaataacat caattgctgg
     2881 tatttctttt gcaatttgga aatcatcctg aatcccgaga tgactcatca aaactaaaac
     2941 atccacttgt gaacgtaaat cttcgactaa ttctggcaaa atatcataag gattccgtat
     3001 gtcccagcca ttcggattat aagtaagcgg aaatggagca gtaaatgcaa ttagtccgat
     3061 tttcgtctgg tgtttcgttg taatgatttt atatggtttc gtccatgccg gtggctggag
     3121 cgtattttta tcgaataagt tatccaggat gatatcaaac tcagcatgat cgtataaatg
     3181 attcaactct tctttggaat ttcctacgcc ttcattgttt cctatcgtcg ctgcatcata
     3241 accaacttca ttcatcagca agatattttc ctgtccattc gttgcttctg acaatggatg
     3301 ccaacgatcc acaaaatcac ctagatctac tgcaatcgtt gtcgtttttt ctttcttttc
     3361 gttttctcgt ttacgttgat ccaaaaacct tctgatcctt ggccaatttt ctaaatgaga
     3421 atgcagatca ttcgtatgta aaatcactat tttttccatg taccctctcg cctctcttta
     3481 tctttatcat accataggca gctttttctt ccattgaaac aagctctatt tcagagtttt
     3541 tcctgccttg aagcaaattg tataagtaca atattcgata aatcagtctg gctaaaaaat
     3601 caggaacatt catttttaca gtactataaa aataaaaaaa acagacccga aagtctgttg
     3661 tcgtttcatt ataatcagac acatggcgga acctgcttag tgctgcttcc ttccagacct
     3721 gacacgcttc acaagcccac cattgtccta gcctttcggc aaagaatagt ataccttatt
     3781 tttcattcgt tgaccagtct ttttttaatt tcttttttcg ctggcggagc tttcggaaaa
     3841 agtcagtcaa aatcatccca cattcgtctt ctaaaatgcc accttctaca tatgcttggt
     3901 ggttaaaccg ctcgtcctcc aaaagattca tcaaggtacc ggctgttccg ccttttggat
     3961 catacgcgcc gaaataaact tctgctacac gagacaaaat catcgcgccg ctgcacatag
     4021 gacaaggctc taaggtaaca aaaagtgcag catcttccaa gcgccagctt cccacttttt
     4081 cacaggcttc cttgattgca agcatttctg catgtgtagt agcatcttgc gtggtttctc
     4141 taaggttata tcctcttcca atcacttttc catccaatac aaccactgct ccaatcggaa
     4201 cttcatgaag catttctgct ttctttgctt ctgccaatgc aaggcgcatc catttttctt
     4261 tttctactgc atcaagattt gtatttacca attacccttc cctgcctttg ttttttcttg
     4321 ttttacttta gcatatgata aaaaggctgt aactaacttt ttttctatgg taaaataaat
     4381 acaagaggtg aacaagatga aaatggataa attacttgct ctttacccac aagcacagaa
     4441 aaaaaactct ccagcatcag aagcggatat cctttctttt gcaattgatg atgcttttat
     4501 ctggatcaaa caagattcat tatctcaaca agaaaccagc cttttgaaag cgttattccc
     4561 tgtaatcaac gatcaaaaaa aacacccatg gtatcactat ctttttaaag atgcttcttg
     4621 tgttcaagaa aaaagctttc gtttgatcca gcttcacgtc gaatcaaaag gagaattctt
     4681 aaaaaaagaa tggcaagata ctattttgga attatttcgc tattctgctg atttcttttt
     4741 ttatacagac acagatgcgc tcttagtcga acaaataaac cgtgattatc ttgatgctac
     4801 tgaaatcaac ggtatttttc tatctttaga tgctgatttt gatacgacaa cgtctgtatt
     4861 cgttggttct ttccattcac ctggcaccga cctcgtccct ttgtttgctg aagaaagaaa
     4921 aattttttta acagagcaaa acaatcttta tactcacagc cgtacttttt ctatgcaaga
     4981 tgtggcttta cattactata caaaagatac tgtaaaagat agtaagttga tcaaaagtta
     5041 catagaactg atcaaacaaa atgaaatgga agtgatcatt caagaattat ggaatgaatt
     5101 aggcaatgtc agctctgctg caaaaacact gtttcttcat cgtaatactc taaaatatcg
     5161 aatcgaaaaa tttcaagaac agtctggttt caatctaaaa aaagccaacg atttactgtt
     5221 ttgctatctt ttattgcttc atgaacataa ttaaagatag attaaagggg ttgtgacaat
     5281 aattttgtca caacccctta tttcgaatga atagtgttca aaagctagtt tctcttacat
     5341 cattttttca acaaaatgca ttagccggtc ttttacatct tcggttgaat ctttgattga
     5401 atcaccataa acagagattt tcttttttcc tgatttgatt tttgctaaca ttcccatcaa
     5461 agaatccaag tcactatctg aaagaccatc tacgatttct agtaattttt catttccatc
     5521 aaatttgtca tagacgaatt tttttacttt cgtccgagta gtcatatgat ggatcttttc
     5581 cattactttt cccgatacaa cgactgctgt cgctacactg gcagcggctg ctacgcttaa
     5641 tccgattgtt acttttgttg atgttttcat aagtatcagc tcctttttat ctatctccat
     5701 catagcatag acaaaaaaat aaacgaacta atccactcag tgaaatctca atcctttagg
     5761 gaaaaagttc tttactgttc cttttacttg tttcccaaat ccgacaggta tcttatctaa
     5821 tactagcaaa acccatcctt gattcccatc acgttgaaat gtttctcctg ctgtgtaaga
     5881 ttgccattcc ttttgagtga tcggcaagca aggtatattc tctatctttt ttgtagcaag
     5941 cgctaatgca tagctaggtt caaatcgatt ctttttgaaa tctcctaaat gcaaacctgt
     6001 acgtacgact tttaaaccat cgagagaagg ggctaactca ggaacttccc aaagatggtc
     6061 gttgaacact aaaagtcttc cagtagcttc ataatgaaaa tcatttgaaa actccgtcca
     6121 gagtttttcc tgctcttttg tcatttgcac tttacttttt tttcgtgtct tcttttcttt
     6181 atgcatctga ttttgtccat gaaaagtcag ctttgcaaca aaatgtcctt caccctgatc
     6241 tttatgaggc catatacgta ttgttttttc caaaccagct acgcttcccc attcagaacg
     6301 tcctgaacta acggattgag tcaaaggaat ctcctcaatc gttactggat aattttctac
     6361 aagccagcta atgatctctt cattttcttc tggtgcaaac gtacaagtcg aataaatcag
     6421 ttgtccttta ttttttagca tttttattgc tgaagacaaa atctcttgct gacgtttttg
     6481 acagtatagc ggagattctt ctgtccactc ttttattgca tttggatctt ttcggaacat
     6541 tccttctcct gaacaaggag catcaaccac gatccgatca aaaaagcccg aaaaatgagg
     6601 gacaagttct gcgggtgcat gatttgtcac tattgcgttc gaaactcccc atcgttctat
     6661 attttctgat aaaatcttcg cacgtttcgg aaagatctca ttcgtcacta ataacccttt
     6721 ccccttcatt tgagcagcta attgggttga tttaccaccg ggagccgcac acaaatccaa
     6781 aactttttca ccgggttttg ctgcagcagc tgttccaacg atcattgcac ttggttcttg
     6841 gctatattca tacccagctt gatggaggaa agactttcca ttgacagtgc caagaaatcc
     6901 ctcattgctg tagggagcgg gttgtaattc ttcgctatgg taagtttgga ccatgtccaa
     6961 tcctgccggt tttagtggat tccaccgaaa tccttttttc acgcttcctt gttcaagcgc
     7021 agaaaaaaaa tcggatgctt cttcgcctaa aagaagacga tattttttga taaattgctg
     7081 tggtaacgtt gtcgcttcct ccttcattcc aatgtttcgt tagacatttt acaatctttt
     7141 ttacgaaaaa taaaggtggc tgatctactt tttcatgttg ctgcctaagg tgactgttcc
     7201 tttaagatat tttccaagta ttcatccgtc acctcgtttt tatcccaaac agcgttcatc
     7261 atttcaagaa aatcttgaga agaatcatct gagcgcaaac ttcccaatgc attaagccat
     7321 accaattcaa gcgacttttc ttctttgatc ttcgtgtgac ctttgacata tgagaaaaat
     7381 ggccgcttaa atagcgaatc catgtctaaa tcagattttg caatgatttc acgaatactt
     7441 tcatggatag aaaaaaaatc tttcaataag tagacaatgc ttaaaacatc atcggaactg
     7501 cattcagcta agtagttttc aaaataaaca gtgatttctt ctaatatctt ctcttttaac
     7561 ccgctgattg atttgaaatg tgtataaatc gggtatgtag aacaaccgca gaactctgct
     7621 aactctcttg cagtaattga cttcattcca tattcttcca cgtataccat acttttgaac
     7681 agcaaatatt ctttactata atttgttact ctccccatca ttcttacctc cgtctctttt
     7741 catctaccca taaataaata acataaataa ttctaaaaat attattgata attatcaaaa
     7801 catatttagt tatatccatt aaaaatcaat ttttccgcct taaaaacaag aatatcttga
     7861 caaaataccc taggaaataa acaatagaat aaacaaggta acaaaaaaat taaataacgt
     7921 tatataaaaa agaaggcgac actctgtgag aataacacag actgaaatga ctttccactc
     7981 acagtttttg tctaactata tacaaaaaca gaataaaaag gaggtattta tgctaattga
     8041 agcaagaaaa aaagagttac ttgttctaca aatggtcatt caaaaaaata attgttcttt
     8101 acaagaatta tccaatgact tacatattcc aaaacggaca atcaaagaac tgatccgaaa
     8161 aataaatgta tcactcaagc aatttttatt gattgatgaa tttatttatt ctaatcataa
     8221 aggagagatc caaaccaaag aaaccgatca aactaaaaag ttagctgttt tttctaagtt
     8281 aaaattatgc tacctaaaag aatccaatcg ttttaactat ttgatgttac taatcaatta
     8341 tccacaaact gctgtaccaa aaaaatattt acttgaacaa ctttatatat cgccctccta
     8401 tctcgaaaaa ctgacaaaac agctcaatca ttccttaata aaattccatt tacggatcct
     8461 atctgtaaaa aattgctgta ctcttgaagg aaatgagtta tctatccgtc tctatcttta
     8521 cttttttctt tcagatacat ttggaggtaa cgattggcca ttttcctcac ttgaattaaa
     8581 tgcactaaaa atgaacagta cagatgagca aatagaaaaa atttcaaaac aagaaaaaaa
     8641 tatctacttg ctgttaactt tattcatatt acgaaacaaa caacaggcat attttccaaa
     8701 aacttcttct gaaatcactg aattgatgga cttattgcag catacacatg attattcaga
     8761 atactttact tctttttcag tgagtcgccc ttgtgaagag acagaaacag agagagccta
     8821 ctttaattat tttttctatt gtttttttcc aaatcaaatc tcttttcgac aaaaacagga
     8881 gattggtttt ttattttcca caagcaaaaa ccttatttgt cgacaagtcg gtcaattgat
     8941 gacttactta actcaacagt ttcctctcaa ccatttagaa aataaaatac caatttattt
     9001 ttattttctt gtgttggcag ttgttttttt cgaactgttg ggtgaatcca gcagtagttt
     9061 ttctacactt tgctttccag aagccacgtc caatcattct agttctttta tacaggaaaa
     9121 gaaattaaga gaaaaaatcg atacaatttt ttcaaaaaat ggggaaagaa accaaatcag
     9181 tcaatacctt agtcaattga tccaacactt actacaaaca gaaacgaaag agcagttact
     9241 catccatttg cagataacca aacagtttac aggaaattat catcttcaaa gccatttgca
     9301 gcagttattt cgcaaagatg ctgttaaaat taccgcatat tcagaacatg ctgatttgat
     9361 cattacagat cagcatgaga aaatttcaga tgaaacggag actttctatc tggatacaac
     9421 gcacaataca gtgttatttg gcaagttaat aaaagcggtt catgaactgt atatgaaaaa
     9481 aggttatgag aacaaaacaa agaggatgtg acaaaattct tgtcacatcc tcttattcca
     9541 aataaatggt attcaatcag ctaactctcg gtattagctc agatcaaagc gctgatttca
     9601 caacttcttt attttaatct cttcggttgc cgccgaacaa taagatcaag cgcaacagct
     9661 gcaggaatgt cgaaagcgct gctgcaacat aagtcagtgc tgctgcaaat aatacgtgtc
     9721 ttgccatcgg tacttcttct tccgtcagta gtccgccttc actcaaaatt tggattgctc
     9781 gccgagacgc gttaaattcg actggtaatg tcaccaattg gaaaagtaat gctaaagaaa
     9841 aagcgaaaat cccgatattg attaacgtag agttccagct aagcaataca cctaccataa
     9901 tcaaggggaa cgaaatcatc gaaccaaagt tagctgctgg aacaagtgct gcacgaatct
     9961 tcaatgggac ataacccgaa gcatcttgta ccgcatgtcc gcattcatga gctgctacac
    10021 ctatcgcagc aactgaagta gactgtgcag ttgcttgaga caaactcaac atcttattac
    10081 ctgaattgta gttatctgtg agatctccgg caatttgttg aacaccgaca ttatttatct
    10141 gttgactttg taatatatat tgtgctgctt gtgtaccagt cacattgttt ttgcttctca
    10201 cttgatcgta ttttcggaaa gtactattga cgtacgctga cgcaattccg gaaataacta
    10261 atccaagaat gaccaaaaga aatgtcggat ctaaaaaacc atacattcca taaccataca
    10321 ttttcgtttc actccttttc gtttacttta ttttatcatt agtagtagga aaagaatgtg
    10381 aaaagacctt gaatattctt taattctttt tcacaaattc cgatttcagt ttcatcggtc
    10441 caaatccttc taccttgcaa tcgatattat gatcgccttc tactaaacgg atgttcttga
    10501 cttttgttcc ttgtttaatg gcacctgacg caccttttac tttcaagtct ttaatcactg
    10561 tcacgctatc gccctctaac agacggtttc catttgcatc taataccact aatccgtctt
    10621 ctatgacagt ttcttgttct tctttgttcc attcgttccc gcattctggg caaatcaaca
    10681 agcctcgatc ttcatatgtg tactctgagc cacattctgg gcaatttggt aatgtcatct
    10741 tctttctcct ttaaaatctt tagtgagcac tattgtaacg gaaaaagaag atcttgaaaa
    10801 gatacatttg tgatttttac tacaaaaggc acgttttcga acgaagtatc attctgtttt
    10861 tctgctctta tacgattggc atatatcttg gatctcttta tagatttcct ctacttctgc
    10921 taaatctttt gcattcgcac cacttgctaa tggatggcct cctccatgat ggcgtttagc
    10981 caattcattg atgatcggcc ccttagaacg taaacgtacg cggtaatacc ctgaaggctg
    11041 ctgaacaaaa atcccccaag ccagcacttc gtcaatcact ccaggaagtg aaacgatcga
    11101 agctgtctct gaatcatcga ttccatattt atcaagaatt tcttgcggaa ggattattct
    11161 tcctgcacca ttttcatcca cctggatatt ttgataaaga taaccggaaa gtttagcgac
    11221 tttcatcggc atttgttcaa tctctctgtt caaagcactt gcatcaaatg gataatttct
    11281 caatatcgaa gcaatctcta atgtacggga cgtcgtggag ggatacaaaa agcgccctgt
    11341 atctccgacg attcctgcat aaagtaaccg tgcagcctta acgttcatct ttaaagattc
    11401 aggaaacatc aaagcaaatt ctgcaatcat ctcactacag ctacttgctt ttgtatttac
    11461 ccacacaaga tctccataag ggtcatcatt gggatgatgg tcgattttga tcagtttttc
    11521 tgccaactga tatctttgat cactgattct gggtgaatta gctgtatcag tcacgatgac
    11581 tagcgctcct tggtagaaga catcttcaac ttcttgcatt tctgctaaaa agcgaagccc
    11641 ttcaatcgtt tcacctactt gacgtatttc tttttctggg aaactttccc gtaatagttc
    11701 ggctaatcct acttgtgatc ctaatgcatc aggatcaggg cgcaaatgcc tgtggataat
    11761 gattttatcg tattttttta ttgtatctac tatttcttgc tgtactgtca tttcactcct
    11821 gctttctaag aacgttccat tacttgacaa acaacgattg ctttggaaac gatggtgttt
    11881 tcaataaaca cttcgatatc cagtttagcg gaacgtctgc cgatttccaa tacacgtgga
    11941 cgaatgtcta attcgctttc caattggatc agccgcaaat aatgtaggtt cacttgttca
    12001 atcaagatat ttcgtttttg gttcatcacc attgtttttt gtgcgacatc agaaatgatt
    12061 tcgctaagta cgccaaaaga aatggttcca acgccattga ccatttgtgg tgctactacg
    12121 aatttgaact tagggttttc caaacgattt ccttcacggt cagtatcaat cgtatggata
    12181 tttccagata tctgatccga aatcgtatct gcaatctgcg gttgtcgctg gacaagctgc
    12241 atcgctttca ttacatcttg ccgggtaatc aagccttcca atgtcaagtc atcggaaacg
    12301 accggcatga cttctagccc atcccagatc atttgatggc taacagaggc aatactcatt
    12361 gttttcttta cactgattgg ttctttcgtc atcacccgat cgatgatctg gtttggcgat
    12421 ttccctaaaa catcttttgc cgtaacaatc cccatcaaac gatgatggcg gttgacgact
    12481 ggaaaacgcg aatgctgcgt ttcttctgac aaacgctgat aatcagaaat cgtatctgtc
    12541 tgctgcaaat aatgagtctt ttcagctggc atatagatat cactgacaag catgatatct
    12601 tttttgatca attggtcgct taacgctcgg ttgatcatgg ttgcgactgt aaatgtatca
    12661 taagaggtgc gtaagatcgg catttcaaga tcatcagcta gctttgcaat ctcagcagtc
    12721 gtattgaatc ctcctgtgat caatactgcg gcgccatctt ccaatgctaa tttttggact
    12781 tccgcccggt tgccgacgat cattagtgat cctggcgtaa tatagcgttc catggcatgt
    12841 ttagtcatcg cgccgatgac gaatttattc aagaccttgt ctaatcctgc cgccccgcct
    12901 aaaacgtccc cttcaatgat cctgacgacc tcgccaaatg tcaaacgttc aatatgtttt
    12961 tttaatttgc gttcgatacg gatggtccct actcgttgaa ttgttgaaac tagtccgaca
    13021 ttttctgcat ctttgattgc acggtacgct gttccttcac tgacattcaa ttcttttgcg
    13081 atgcttctta cagaaattcg attgccaaca ggtaaacttt caatatattc taaaatcaaa
    13141 tcatgttttg tcgccatttt ctgcctacac ttccagcgtt tccgagacgc tcattatttt
    13201 tccttcacta tcttccagta actctacaaa agattcggga tcttgttcaa tgacagggaa
    13261 agtattgtaa tggattggaa ctactttctt ggcacgaatt gtttgcgcag cctttgctgc
    13321 atcttctggc cccatcgtat agttatcgcc aatcggcaag aaagcgacat caatatcgaa
    13381 ttcttctgca agcaatccta gatcactgaa ataagcagta tcccctgcat gataaatcgt
    13441 ttttccttct gcttgaataa taattccgct aggctcgccc atatactgaa cgataccgtc
    13501 tacttcatag cttgaactat gctgtgcatg tacgaatttt accacgccga atggaaacgc
    13561 atgttttcca ccgatattca taccgaatga atctactcct ctgctgttcg cataatcagc
    13621 aatctcaaca atactgataa ttgtggcttg attttctttt gcaataggaa tcatatctcc
    13681 tatgtggtca ctatgaccat gagtaatcag caaccaatct gtctttactt cttctgcagt
    13741 taagtctgtc aatggatttc ctgtaataaa cgggtcaatc atcacgcgtt ggccattttc
    13801 catttcaatt gttaaacagg aatgtccatg atacgtaatt ttcatatttt acacctcgcg
    13861 ttttctctgt atctttttta ttaaaaacga tttcatcact tgcacatcaa tgaaaaaata
    13921 tagctgctaa atatattttc attggattga tagtacagta tgtacaaagc aattgtaaca
    13981 gataatacag gatgcaccaa gtaccaagca gatataatct caagtttgag gtctctgctc
    14041 tgcttagctt ctcttctcgt cccttaacta attaaatcag cgtaaatagg atggacgatc
    14101 ttttctaaat cggctacttt tatccgaagg cttctcccga tttcaccgct tgacacaatt
    14161 acttcttctt tttcttttaa agaactgtct gctaaaaaac aataatcagg atgtgcgagt
    14221 tttattccaa taggggtgtt tgctccgtgg ggatatcctg tatgcattaa tacaaaatcc
    14281 attccaggaa aaccaacttt acgatctttc gtcacttgtc tcgttttttt gtaatctaag
    14341 tgatcgttca aaggaatcaa tgcgatgatc accccagttt tgtttccttt cactacgaga
    14401 gttttacata tcaaagctgg atcaatccct cttttttgta gttcttctgt gaaagattct
    14461 tctaccacat gactaaaatc aagtggttga tagctgatct tttgttcttc taaataacgt
    14521 tcgacttttg tttgactcat cattgattca ctccctttat tctatcgttt attataaagc
    14581 gagggatgct gtattcaaaa attatttgtt tttcgcaaaa aaagagcaag acgagcactc
    14641 tatagagagg ttcatcttac tctttttgtt tattttttat ttcgacgttg agtagcttcc
    14701 ataatgactg gcaagatcac tgggcggcgt tttgtttgtt caaataagta acggcctaat
    14761 tgatctcgga tgtcttgctt taatttgctc cactcaaaat cattgctttc taaatgtttc
    14821 tcaacgattg tttcaatcat attgctgctt tctttcatca gatcacggct agcttttacg
    14881 taaacgaatc cccgtgaagt aatttgaggt tttgaaatga ttttcttttc tcgccgactg
    14941 atagtgacaa ccgctacgaa aatcccgtct tctgacaaga tttttcgatc acgtaaaaca
    15001 atgtttccga tatcacctat gcctaatcca tcaatcatta cattttctgc acttgtagtt
    15061 cctgcaacac tcatacgctt gttcttatat tccagcacgt ctccgcgtcc agtaatatag
    15121 atatttttgt atggcatccc tactgcatga gccaaatctg catgagcagc taattcacga
    15181 tactctcctt gaactggcac aaaatatgtt ggtttcatca aattgatcat taattgcaag
    15241 tcattaggat tggcatggcc agaaacgcgc atattctcag agatcaactt cactgttcca
    15301 ccagctcgat aaatgatatc ttccgttttc gccacgatgg tttccatcgc gatgcttggc
    15361 gtagtagtga tataaaccaa atctccttct tcaatcttga cattacggtg tgtaccgttt
    15421 gccatttttt gcagagactt gatgggttca cccattcggc cagtttctaa aatgatcagt
    15481 tcttctggat catagttttt catgtctttc aacgtaacaa gcagatcttc acttggtaat
    15541 tgaagttttt ctaaacgcat cgctgtgcga ataattcgtt cgaaatcttg accagtcaag
    15601 acaacttttc gttgtgcttt ttctgctgca tccaatactt gttgcactcg ttgcaaatta
    15661 cttgctacac aagcgacgat gatacgccct tcccaatatt tgatcgtatc aaatacttca
    15721 tctgctattt gggcttcaga agctacaggc tgaggatttt ctgcatttgc cgattcactt
    15781 agtaaggcta agactccttc tttgccgatt tccgccaatc gtgcataatc tgtttgatac
    15841 attgggatag ccgtttggtc aaatttaaaa tctcccgtat aaacgatact accttctttt
    15901 gttttcagat tgatccccac tgaatctggt atcgtatgag tggtacggaa aaagctcact
    15961 actgtttcac caaagtctat ttctgtatgc tcatctacca catggaaatc cttaaatcct
    16021 ttggctgctt cattacgact gacattcaat tttgctaact cgatcgtcaa ctctgtcccg
    16081 aatactggta cagaaatttt tgacaacaag taaggaagtg ccccgatcgc atctgcatgt
    16141 ccatgtgtta aaaagacacc agctacacga tcaatgtttt cttctaaata cgtaaaatca
    16201 gggattacga cgtcgatccc caacaactca ttttcaggat attttagccc acaatctaaa
    16261 ataaaaatat cctcgcccac ttctgcgaca tacatgtttt ttccattttc acgaacgccg
    16321 cctaatggga taattttaat ggtactcaca aacttcacct cttcttatga catccaagtt
    16381 taaagatgtc gattttcttc tttatctaaa tatatgagcc attccaatga agcatatgca
    16441 aaatcactat ttctcaactt atggagaatc agcaggaaaa ttaccagcac atccgcgttc
    16501 tgacagttgg atgggcattt tattttggat atacttcatt aacaaaatgg atcttttgcg
    16561 aaacgctata gccagaatac ttatataagt attcagctgt tccccattat tatacaacaa
    16621 ataaactaaa tcgctagtat cagcaattgt tagaacaact atgcacgtaa aaatccctct
    16681 gtagcatcta ggatttcttt tgctattcct ctttcatttc ttcttcgtcg gtttgaaaca
    16741 tacttttttc attttaccac attttttgca ataactatag tgacttttta tatatacaga
    16801 acctcatgtt gatggtgtgc aaacagcata agatttaagc tagctttaag ataggttgtc
    16861 tattatcaaa tgcatctact aggagggatg caaaatatga attttatgaa acgtgcatgg
    16921 aaaagtacaa aagtaaaatg gggacgttca ttgttattgt ttgccgtgtt cacagccatt
    16981 cttatttttg tgctagctgg cctcacgatt cgaagcgctg cagaagaagc agcgaatcaa
    17041 gcacaaaaag aagttggagc aacagtgact ttaagtgcca atcgagaagc ttcatttaaa
    17101 aaacaggaac aaacttcttc aagcggttct accactgaag aacgtccaga tccaggcagt
    17161 ttttctctta caccagtttc cttaacggac gcggaaaaaa tagctgaatt agataatgta
    17221 aagagttatt cctttgaaag ttccgcctca gctatggcaa aagatggaat aactgccatc
    17281 actagttctg attcaagtga agacagttca gaaactactg atgcaggcgg tgaacctgct
    17341 ggaggaatgg gcgggaatcc gcaaatgact caagcagact ttcaaatcag tggtgtgtca
    17401 gcaactgcac aaaatagtaa tttttctgat ggaacagcaa aaatcacgga tggtgaagga
    17461 atagatgaca gcgacaaagg cacaaacaat gtagtcattg attctactct agcagaaaca
    17521 aatgacctgg cagtcggaga tactttcaca atcacgagta ctgaagatga agataccact
    17581 tatgaaatga cgatcaaagg aatttatgaa tccagcgaaa caagcggcag tatgggtatg
    17641 aaattcaact tcatgaatcc gtcgaataca ctttacactt cttacacttt tgcaaatgaa
    17701 ctaaatggga catctgacga taacacgata gattctgcta tttacactct atctgatcca
    17761 gataaaatgg atgagtttct caaagaagct gagaagctga tcgacacaga tacatttagc
    17821 ttacaatcaa atgattccat gtatcagtca atgttggaac ctttaaacaa cgtggcaagt
    17881 ttttctaaaa atgttgtttt actagtggct gtagccggta ttatcatact tactctgatc
    17941 attatgatta ccattcggga gcgtcggcat gagttaggcg tcttgctatc tttaggagaa
    18001 tccagaagta aaatcattct ccaattgttt accgaagtgg ctatttgtat gattcttgca
    18061 ttaggagttg cttcgcttag cggaaacgtc gtcgcgaatg ccgtgggaca gcagctactt
    18121 gaccaacaga cagaaaccac gacacaaaac cagcaagctc ctggcggtgg acaaatgcca
    18181 ggtaacagac aagaacaagg cggtcaacgc gatggtcaac aaggaaccac gaataatcca
    18241 tttgctgttt ctgatcaagt cagcgaattg gaaatcaatg tcaaacctgc acaattggga
    18301 ctattagccg gagtagcatt tggaatcagt ctgttttctg tctttttagc ttctataggg
    18361 atattaagat taaacccaag aaaaatccta ttaaactaaa ggaggaattt gatgttacgg
    18421 acagaacaat tgggattttg gtatcaagat ccgcaagatg cgctttataa agatgtagaa
    18481 ctgaatttcg aagctggaaa aatgtacgct attttaggag caagtggttc tggaaaaacg
    18541 acttttcttt cgcttatttc tggactagat gcaccaaaag aaggaaaggt atactatcaa
    18601 gatcgttcac tgaacaaaat cgggttaaga agttatcgta aaaatgatgt ctcgattatt
    18661 ttccaagctt acaacttgct tccttattta tcggctctag acaacgtcat agcagcaatg
    18721 gaaatcacta agaccaagca agagaattat cgacaagttg ctttagatcg actggctcgt
    18781 gtaggaattg atgaaatatt agcaaaaaaa caggtgacca aactttcggg aggtcagcag
    18841 cagcgagtcg ctatcgttcg tgctatctgc tgtgaacatc aactaatcgt agcggatgag
    18901 cctactggaa atctagatga aacaacttca caagatatcg tcaaactttt ccaagaaatc
    18961 gcccatgaac aaaatcgctg tattatcatc gtgactcatg agattgaagt agcaaaaagc
    19021 tgtgatgaag tctttgaact aaaaaataaa aaatttcaaa aaatcgaagt ttaaaaaaca
    19081 ggataatgat caaataaaaa a
//
LOCUS       DADVXN010000051        19016 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.053, whole genome shotgun sequence.
ACCESSION   DADVXN010000051 DADVXN010000000
VERSION     DADVXN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.053"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(80..289)
                     /locus_tag="KB942_001553"
     CDS             complement(80..289)
                     /locus_tag="KB942_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851981.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(300..1627)
                     /locus_tag="KB942_001554"
                     /pseudo
     CDS             complement(300..1627)
                     /locus_tag="KB942_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1763..1918
                     /locus_tag="KB942_001555"
     CDS             1763..1918
                     /locus_tag="KB942_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851982.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1992..2579)
                     /locus_tag="KB942_001556"
     CDS             complement(1992..2579)
                     /locus_tag="KB942_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBB6851983.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2662..3912)
                     /gene="clpX"
                     /locus_tag="KB942_001557"
     CDS             complement(2662..3912)
                     /gene="clpX"
                     /locus_tag="KB942_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBB6851984.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALSAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4159..5448)
                     /locus_tag="KB942_001558"
     CDS             complement(4159..5448)
                     /locus_tag="KB942_001558"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBB6851985.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5602..6534)
                     /locus_tag="KB942_001559"
     CDS             complement(5602..6534)
                     /locus_tag="KB942_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851986.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(6691..7350)
                     /locus_tag="KB942_001560"
     CDS             complement(6691..7350)
                     /locus_tag="KB942_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6851987.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(7353..8378)
                     /gene="sppA"
                     /locus_tag="KB942_001561"
     CDS             complement(7353..8378)
                     /gene="sppA"
                     /locus_tag="KB942_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBB6851988.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(8391..8681)
                     /locus_tag="KB942_001562"
     CDS             complement(8391..8681)
                     /locus_tag="KB942_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851989.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9090..10895
                     /gene="glmS"
                     /locus_tag="KB942_001563"
     CDS             9090..10895
                     /gene="glmS"
                     /locus_tag="KB942_001563"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBB6851990.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(10967..12322)
                     /locus_tag="KB942_001564"
     CDS             complement(10967..12322)
                     /locus_tag="KB942_001564"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBB6851991.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12346..13515)
                     /locus_tag="KB942_001565"
     CDS             complement(12346..13515)
                     /locus_tag="KB942_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6851992.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(13512..14399)
                     /locus_tag="KB942_001566"
     CDS             complement(13512..14399)
                     /locus_tag="KB942_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBB6851993.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(14588..18280)
                     /gene="nifJ"
                     /locus_tag="KB942_001567"
     CDS             complement(14588..18280)
                     /gene="nifJ"
                     /locus_tag="KB942_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBB6851994.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            18479..19000
                     /locus_tag="KB942_001568"
     CDS             18479..19000
                     /locus_tag="KB942_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6851995.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         5322 a         4089 c         3381 g         6224 t
ORIGIN      
        1 attataaatc agtcaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
       61 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
      121 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
      181 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
      241 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
      301 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
      361 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
      421 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
      481 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
      541 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
      601 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
      661 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
      721 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
      781 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
      841 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
      901 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
      961 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     1021 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     1081 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     1141 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     1201 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     1261 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     1321 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     1381 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     1441 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     1501 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc
     1561 tgtttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
     1621 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
     1681 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
     1741 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
     1801 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
     1861 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
     1921 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
     1981 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
     2041 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
     2101 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
     2161 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
     2221 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
     2281 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
     2341 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
     2401 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
     2461 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
     2521 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
     2581 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
     2641 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
     2701 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
     2761 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
     2821 agcaccggta ttgcgttcga ttgctttttt cgcaatggca ctcaacgctt caggttcgaa
     2881 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
     2941 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
     3001 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
     3061 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
     3121 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
     3181 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
     3241 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
     3301 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
     3361 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
     3421 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
     3481 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
     3541 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
     3601 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
     3661 cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag
     3721 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
     3781 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
     3841 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
     3901 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
     3961 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
     4021 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
     4081 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
     4141 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
     4201 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
     4261 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
     4321 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
     4381 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
     4441 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
     4501 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
     4561 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
     4621 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
     4681 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
     4741 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
     4801 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
     4861 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
     4921 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
     4981 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
     5041 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
     5101 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
     5161 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
     5221 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
     5281 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
     5341 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
     5401 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
     5461 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
     5521 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
     5581 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
     5641 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
     5701 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
     5761 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
     5821 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
     5881 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
     5941 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
     6001 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
     6061 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
     6121 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
     6181 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
     6241 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
     6301 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
     6361 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
     6421 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
     6481 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
     6541 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
     6601 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
     6661 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
     6721 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
     6781 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
     6841 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
     6901 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
     6961 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
     7021 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
     7081 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
     7141 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
     7201 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
     7261 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
     7321 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
     7381 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
     7441 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
     7501 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
     7561 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
     7621 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
     7681 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
     7741 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
     7801 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
     7861 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
     7921 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
     7981 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
     8041 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
     8101 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
     8161 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
     8221 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
     8281 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
     8341 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
     8401 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
     8461 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
     8521 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
     8581 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
     8641 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
     8701 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
     8761 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
     8821 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
     8881 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
     8941 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
     9001 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
     9061 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
     9121 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
     9181 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
     9241 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
     9301 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
     9361 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
     9421 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
     9481 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
     9541 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
     9601 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
     9661 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
     9721 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
     9781 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
     9841 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
     9901 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
     9961 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    10021 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    10081 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    10141 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    10201 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    10261 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    10321 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    10381 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    10441 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    10501 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    10561 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    10621 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    10681 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    10741 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    10801 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    10861 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    10921 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    10981 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    11041 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    11101 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    11161 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    11221 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    11281 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    11341 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    11401 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    11461 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    11521 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    11581 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    11641 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    11701 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    11761 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    11821 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    11881 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    11941 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    12001 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    12061 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    12121 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    12181 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    12241 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    12301 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    12361 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    12421 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    12481 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    12541 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    12601 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    12661 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    12721 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    12781 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    12841 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    12901 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    12961 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    13021 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    13081 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    13141 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    13201 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    13261 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    13321 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    13381 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    13441 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    13501 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    13561 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    13621 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    13681 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    13741 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    13801 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    13861 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    13921 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    13981 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    14041 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    14101 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    14161 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    14221 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    14281 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    14341 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    14401 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    14461 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    14521 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    14581 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    14641 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    14701 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    14761 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    14821 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    14881 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    14941 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    15001 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    15061 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    15121 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    15181 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    15241 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    15301 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    15361 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    15421 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    15481 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    15541 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    15601 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    15661 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    15721 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    15781 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    15841 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    15901 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    15961 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    16021 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    16081 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    16141 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    16201 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    16261 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    16321 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    16381 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    16441 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    16501 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    16561 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    16621 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    16681 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    16741 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    16801 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    16861 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    16921 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    16981 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    17041 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    17101 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    17161 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    17221 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    17281 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    17341 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    17401 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    17461 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    17521 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    17581 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    17641 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    17701 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    17761 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    17821 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    17881 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    17941 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    18001 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    18061 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    18121 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    18181 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    18241 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    18301 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    18361 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    18421 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    18481 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    18541 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    18601 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    18661 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    18721 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    18781 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    18841 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    18901 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    18961 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtg
//
LOCUS       DADVXN010000052        18938 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.054, whole genome shotgun sequence.
ACCESSION   DADVXN010000052 DADVXN010000000
VERSION     DADVXN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.054"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(162..1460)
                     /gene="eno"
                     /locus_tag="KB942_001569"
     CDS             complement(162..1460)
                     /gene="eno"
                     /locus_tag="KB942_001569"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBB6851996.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1576..2331)
                     /locus_tag="KB942_001570"
     CDS             complement(1576..2331)
                     /locus_tag="KB942_001570"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBB6851997.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2435..3625)
                     /locus_tag="KB942_001571"
     CDS             complement(2435..3625)
                     /locus_tag="KB942_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBB6851998.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3735..4736)
                     /gene="gap"
                     /locus_tag="KB942_001572"
     CDS             complement(3735..4736)
                     /gene="gap"
                     /locus_tag="KB942_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6851999.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4777..5868)
                     /locus_tag="KB942_001573"
     CDS             complement(4777..5868)
                     /locus_tag="KB942_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBB6852000.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(6017..7351)
                     /gene="rpoN"
                     /locus_tag="KB942_001574"
     CDS             complement(6017..7351)
                     /gene="rpoN"
                     /locus_tag="KB942_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBB6852001.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7411..7482
                     /locus_tag="KB942_001575"
     tRNA            7411..7482
                     /locus_tag="KB942_001575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7444..7446,aa:Arg,seq:ccg)
     gene            complement(7674..8558)
                     /locus_tag="KB942_001576"
     CDS             complement(7674..8558)
                     /locus_tag="KB942_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6852002.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8645..9544)
                     /locus_tag="KB942_001577"
     CDS             complement(8645..9544)
                     /locus_tag="KB942_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBB6852003.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9561..10067)
                     /locus_tag="KB942_001578"
     CDS             complement(9561..10067)
                     /locus_tag="KB942_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBB6852004.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10256..10876)
                     /locus_tag="KB942_001579"
     CDS             complement(10256..10876)
                     /locus_tag="KB942_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBB6852005.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10952..11389)
                     /locus_tag="KB942_001580"
     CDS             complement(10952..11389)
                     /locus_tag="KB942_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBB6852006.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11552..12382
                     /locus_tag="KB942_001581"
     CDS             11552..12382
                     /locus_tag="KB942_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBB6852007.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12366..13733
                     /locus_tag="KB942_001582"
     CDS             12366..13733
                     /locus_tag="KB942_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6852008.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13752..14561
                     /gene="yidA"
                     /locus_tag="KB942_001583"
     CDS             13752..14561
                     /gene="yidA"
                     /locus_tag="KB942_001583"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBB6852009.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNNEHGVAEAIKKYVL"
     gene            complement(14604..15047)
                     /locus_tag="KB942_001584"
     CDS             complement(14604..15047)
                     /locus_tag="KB942_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBB6852010.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15181..15552
                     /locus_tag="KB942_001585"
     CDS             15181..15552
                     /locus_tag="KB942_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6852011.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15721..16503)
                     /locus_tag="KB942_001586"
     CDS             complement(15721..16503)
                     /locus_tag="KB942_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBB6852012.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16500..17471)
                     /locus_tag="KB942_001587"
     CDS             complement(16500..17471)
                     /locus_tag="KB942_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBB6852013.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17563..>18938
                     /locus_tag="KB942_001588"
     CDS             17563..>18938
                     /locus_tag="KB942_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6852014.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEK"
BASE COUNT         5334 a         3901 c         3372 g         6331 t
ORIGIN      
        1 ttttcttgta ccattctttg taaacatcat atacagaagc tagttgcaaa aaaactctct
       61 acctgctgat atttcagcaa gtagagagtt cgattactta actaagtgat catactcgac
      121 tatctttcaa tagtcgatca tgtatcttca ttagaaaaga attatttgtt ttttaagttg
      181 tagaaagatt tcaaaccttt gtattctgca acttcaccaa gttggtcttc gatacgtagt
      241 aattggttgt atttagcaat acggtcagta cgtgatagag aaccagtttt gatttggcca
      301 gcgtttgttg caacagcgat atcagagatt gttgaatctt ctgtttcacc agaacggtga
      361 gatacaactg ctgtgtagcc agcttcttta gccatttcga tagcttcgaa agtttctgtt
      421 aatgtaccga tttggttcac tttgataagg attgagttag caattccttt ttcgatacct
      481 tcagataatt tttgagtgtt tgttacgaac aagtcgtcac caactaattg aactttgtcg
      541 cctaatacgt cagttaattt cttgaatccg tcccagtcgt tttcgtctaa tccgtcttcg
      601 attgagatga ttggatattt agaaactaat tcttcgtaga atttgatcat ttcgtcagtt
      661 gttttttcgc cttcgcctga atcagctagt acgtaaacac ctttttcttt gtcgtagaat
      721 tctgaagaag cagcatccat agcaagaaca acgtctttac caggtacata gccagctttt
      781 tcgattgctt cgatgattac ttcaaaacct tcttcgtttg aaccaaggtt tggagcaaat
      841 ccaccttcgt caccaacaga agttgctaat ccgcgagctt tcaagattga agctaatgcg
      901 tggaatactt cagcacccat acgtagagct tctttgaatg ttggagcgcc aacaggcatg
      961 atcatgaatt cttggaagtc gattgagtta tcagcatgtg atccgccgtt gatgatgttc
     1021 atcattggtg ttggcaatac ttttgtgttg aatccgccta ggtagtggta taaaggtact
     1081 tctaggtagt cagcagctgc acgtgcaaca gcgatagaaa cacctaaaat agcgttcgca
     1141 cctaatttac ctttgttagg agttccgtct aaagcgatca tagctttgtc gatagccatt
     1201 tggtcacgaa catcgtagcc aatgattgct tcagcaatga tgttgtttac gttgtcgaca
     1261 gctttagtta cacctttacc gccataacga gctttgtctc cgtcacgtaa ttcaactgct
     1321 tcgtattcac cagttgaagc acctgaagga accattccgc ggccaaaagc tccgctttct
     1381 gtgtatactt ctacttcgat tgttgggtta ccacgtgagt ctaagatctc gcgagcgtaa
     1441 acatcagtaa taattgacat gttttgtctc tcctttgagt ttgttttttc ctaagggatt
     1501 tccctcatat acgattttag ttaattttgc tcattagtgc aaacatttct tgcatttttc
     1561 gcaaattttc aacaattatt ttacagcgtc taacaatgac aagaatgaat ctgcttgcaa
     1621 gcttgcgcca ccgactaaag caccgtcaac attttctttt gccatgtatt cagcaatatt
     1681 ttctggtttt acagaaccgc cgtattggat acgaactgct tctgatactt cttttccata
     1741 aagtttttca actgtttgac gtacaactcc acaaatttcg tcagcgattt gtgcgtcagc
     1801 tgatttacca gtaccgattg cccagattgg ttcataagca ataaccatgt ttgacacctg
     1861 ttcagctgaa agatcagcta aaccagctgt aatttgtcct tcgatccatt cagcagtttt
     1921 gcctgcttca taggtttcta aagattcgcc acagcatagg attggtgtca taccatttgc
     1981 aaagatagct ttcgcttttt tattgatgtc ttcgtctgtt tcatggaaat attcacgacg
     2041 ttctgaatgt ccgatgatca cataatgaac acctagatca gcaagagctg ctggtgaagt
     2101 ttcaccagtg aacgcacctg agttttccca gtagcagttt tgtgctgaga tttttagttc
     2161 tgtaccttca gctgatgtca ttaattcttg taagaataat gcaggagatc cgatgactga
     2221 atcaactttg tcatttgaag ggattttgtt ttttactgct tcagcaaatt cttttgcttc
     2281 tgatgcagtt ttgttcattt tccagttacc tgcgataata ggtttacgca ttgaggaaca
     2341 cactccttaa tttattgaaa aatggctagg gagtcatgaa aactcccttc accttttgtt
     2401 cgattactcg tcagaattga gcaaacttgg tcgtttattt atcgttgatt gctgctagtc
     2461 ctggtaattc tttaccttca agcaattcca agcttgcgcc gccacctgtt gagatgtgtg
     2521 tgaatttgtc agcaaatcct aattgttctg ctgctgcggc tgagtctccg ccaccgatga
     2581 ttgttgttgc gccttctaag ttagcgattg cttcacaaac accgattgta cctttagcaa
     2641 agttactcat ttcaaataca cccataggtc cgttccaaac aactgttttt gcaccttgta
     2701 atgtatcagc aaataatttt actgatgctg ggccgatgtc taaagccatc aaaccatcag
     2761 gaatgtctcc ttcgattact tgtgtttcaa catcatttga aaattctggt gcacagacat
     2821 tgtcgattgg caagacaagt ttgtcgcctg ctttttcgat taattctttt gctagttcca
     2881 ctttatcagc ttctactaga gagttaccga tcttgattcc ttttgcttcg tagaatgtgt
     2941 aagtcattcc gccaccgatc aagattttat ctgctttagg aatcaagttt tcgattacgc
     3001 cgattttatc agaaactttt gcgccaccaa ggattgcaat cattggtcgt tttggttctt
     3061 ctactgcttc accgatgaac ttgatttctt tttccatcaa gaatccagca actgttggga
     3121 taccagttga agcgatacct acgttagaag catgtgcacg gtgagctgta ccgaaagcat
     3181 cgttaacaaa tacatcgcct aatgaagccc agtatttacc tagttctgta tcatttttgc
     3241 tttctttttt accgtcaata tcttcaaatc gtgtattttc gaatacgaca acgtcgccgt
     3301 cattcatgtt gcggattgct tcttctaatt gttctccgcg agtttcaggt acgaatgtta
     3361 cttctttacc taataattcg cctagacgtt ttgcaactgg agctaaagat tttccttctt
     3421 tatcttcttc tgtttttaca cgtccaaggt gagagaaaag gattgctttt ccgccttgtt
     3481 ctagaacgta gttgattgtt ggcaatgctg ctttgatacg agtgtcgtca gtgatcacgc
     3541 cgtctttcaa aggaacgttg aagtcaacac ggacaagaac ttttttgtct tttaaatcga
     3601 tatctttcac tgttttctta gccattttat ttcctcctga ataaacttat tatcaaaaaa
     3661 agcggaagaa gcgcgattgc ttccccgctt ttaaatgtac atgtctataa tttacacttg
     3721 tacgtttgtc tatcttataa gttagcgaag tattctaaag tacgtactaa ttgtgcagtg
     3781 tatgacattt cgttgtcgta ccaagcaaca gttttaacta attgtttgtc gccaactgtc
     3841 attactttag tttgtgtagc atcaaataat gaaccgtaag tcataccaac gatatctgaa
     3901 gaaacgattt cgtctgtgtt gtatccgtaa gattcgtttg aagcttcttt cattgctgca
     3961 ttgatttcgt caacagttac ttctttttca agaactgtta ctaattctgt taatgaacct
     4021 gttggtacag gaacacgttg agcagctccg tctaatttac cgttcaattc aggaattact
     4081 aaaccgatag ctttagcagc acctgttgag ttaggaacga tgtttgcagc agcagcgcgt
     4141 gcacggcgga agtcaccttt aggatgaggt ccgtctagag tcatttggtc acctgtgtaa
     4201 gcgtggatag ttgtcattaa tccttcaaca acaccaaatt tgtcgttcaa tgttttagcc
     4261 ataggagcta aacagttagt agtacatgaa gcacctgaga taactgtttc tttaccagtt
     4321 aatgtttcgt ggtttgtgtt ataaacgatt gttggaacat cgtttccgcc aggagcagag
     4381 ataacaacgc gtttagcacc agctgttaaa tgtttttcag ctgctgattt agaagtgaag
     4441 aaaccagtac attctagaac gatatctacg cctagttcgc cccatggtaa ttcttctggg
     4501 ttgcggttag ctaatacttt aacttctttt ccgtttacgt tgaatgaacc ttcatgaact
     4561 tcaactgttc cgttgaaacg tccttgagtt gtgtcgtatt ttaacaagtg tgccaacatt
     4621 ttagcatctg tcaagtcgtt gattgctact acttcgattc catctacatc ttggatacga
     4681 cggaatgcta gacgtccaat acgtccaaaa ccattaatac ctactttaac tgtcatttaa
     4741 gatttcctcc ttatgaaaat caaaaaaata tttattttaa agggttaccc cttttaaaat
     4801 ctcatttgca gcagcttcat cagtgatgag ccacgtttgt tttggtgcat ttttcatata
     4861 agctcgaatt gctttagctt ttgattttcc gcccgcgatt gccatgatga tcgggacttt
     4921 ttccaaatct tgcaattcta tgccgattct agggacctta taaacgactt cacctttttc
     4981 attgaaaaag tagccaaaag attccgcaac agcatttgct tgtttcagca tgactaattc
     5041 tttttcagtc atcttacgac gtgctgccat gtgtaatgca cgcccaatac tatgaataac
     5101 acaattcgct tcactaatca agtttaagac ttcttggata gatggctcat tgagtaaaga
     5161 attgtaagtt gccagactta attgttccgg aacataaagt gcacggtggt taccgtttgc
     5221 tttagcagcc attcttgcac tgacagaatt tgcctgtaca gttatcgctt caccaatccc
     5281 gcctcttgca gggacaaaca ggttatgccg tttcttattt tccagattag acaattgttc
     5341 ggctaccaca gccatagtag ttcctcccat cacagcgatg atattctcgc cttctggaag
     5401 tagtcggtct agggcttcgc tcagcgcttc gccgaatgca tcagccactt tggtctgctc
     5461 ttcgctgttt cctgaaacaa cgatacagcg ttggttacca aaatattctg ccaactgttg
     5521 ttcggtttga tgcgtaccta aaagctgatc catgaagttt tcaaggcttt gataaacttc
     5581 ttcgccctta gttgtcagcg tcataccgct ttttgaggtg tcgatcaaat tcagattttt
     5641 caaaagatct gtttctgttc gcaatactcg ttctgtgatc cccatacttt ccgataaact
     5701 tcgacgtcca atcggctgca tccaatagat atttcgtaaa attttatagc gttcttgcaa
     5761 cacttcgata atatctggtg caacagattc aatcattttt atttcatcca gcatagcgat
     5821 ctccttagtt ggacacttta cgtccaatgt agacttaaaa cgacccatat acgataaaaa
     5881 aagaaattaa ctattcgaat ttcacatcaa taaagggaaa tctcaacctc acgactgtaa
     5941 gtctaacagt tttgctctcg tttttcaact aaaaagacta gaaaaagtga aatacttttt
     6001 ctagtctttt gactgattag ttatcataac gttttctttt tgatgaactc gggattccta
     6061 ataaatcacg atattttgct actgttcgtc ttgagatcgc tatctcatct tttttcagca
     6121 attcaaccaa tttctggtca gacaacggct tgtttttgtc ttcttgatcg actaattcct
     6181 gcagcttttt cttcgctgta tctgctgaaa ggtcttctgt ctgttctgta ctattcgtag
     6241 gaattcgtgt agtgaaaaat ttttttaatt caaatacacc gaagtctgtt tccaaatatt
     6301 tcccgttgac tgcacgactg accgtggatt catgcacatc tatctcagtt gcaacttcct
     6361 ttaacgtaag aggcttgatt ggacgttcct tgtcgataaa gaaagctttt tgccgggaaa
     6421 caatgacttg agcaacccgc aaaattgtat ccccacgttg taggatcgtt ttcttcaacc
     6481 attcgaattc ctgtttccgt tcttttaaat aattttcagt ttctttatct gcttgttgtg
     6541 acatttgttt gaagtaactc tcttgaaagc gtatatttgg ctgatttttt cggtttgaga
     6601 taacttgtac atttttatcg tcgtccacta atacccggac atcaggaata atgtacaatt
     6661 cgctgctacg gtcgaaaatc cggccagggc ttggactcaa cgtttgtaaa tagtcgaata
     6721 tctgctgaac agcatgtaga tcaacagaaa aacgttgtgc gatttctttc cattttcgct
     6781 ccaccagttc ctcaaaacat tcttccaaca cgatataagc taattccggc gcataatcat
     6841 ctcgttctgt ttgaagcatg agacattctt gcaaactacg tgcacctact ccagctggtt
     6901 ctaattgctg aatcagagtc aacgcatcga gcatttggat aggcgtagct ccaatctctt
     6961 ttccggcttc ttctaaacta atcgtcaaaa atccgttcaa atcgatgtat tctacaagat
     7021 aaagaacgat tttcctcaaa aaagtatctc ggtaattcaa atggacttgt tcgatcaatg
     7081 aatcaaataa agaccctttt gtatcaggaa tctgatttag gtaatttgtc tcttctcctt
     7141 cataagaaga tgaagaagct tttatgtaat ctggctgcca ttccggctca acaacttcca
     7201 ccaatggatt ttccattgct tgattctcaa cgaatgccaa cagctcctca ctgttatatt
     7261 gtaaaacttg gatggactgt tgcaactgtt gcgtcatggc tagtttctgt gtctgctgct
     7321 gtttttgtga aaaattttgc tcaaatttca tttttttgtc caatcccctt gtaatttttt
     7381 atctgtttag ttacaattat cccagtgctt gcgtccttag tgtagtggat atcacgtaag
     7441 attccggttc ttgagacggg ggttcgattc cctcaggacg cgttctactg ctattttagc
     7501 ataatttacg aatagttgct tctttatttt taagtgaaaa ctgtccgcca aagaaaattg
     7561 gtggacagtt ttttgttgta cctgtataga tattgttaag cctttgcaag taaggtagtt
     7621 atgtgttcaa ctacttatta aaatgtgtta tttattaaag atacttgtct cacttactga
     7681 actggccgat gatcgtttct gccataatag ttctcgacag ccgttggatg ccctaataaa
     7741 tccccaactg tcgtgatgcg atacccttgc tctttcagat aatcgatcac ttgaggaacg
     7801 gcacgaatcg tttcaggata gatatcatgg aataaaataa ttgccccatc gtatactgtt
     7861 gcttggatct tctgcaaaat caaatcagga tttcttgtct tccaatcttc tgaatcaaca
     7921 gaccattcga tgatcggtcg ctggatgatc gttgctccta cattcgtgac agaaccatag
     7981 ggcggacgaa caaaagtggg ggttttgcca gtgacttttt tgatctcagt gtctgctttc
     8041 gtcacttcct ccgtcatcga ctgggcagaa agagtggcca gattctgatg atcgtatgtg
     8101 tgattgccaa tctcgtgccc tgcttcagct gtctgcttca cgatttcagg atgctgcgcc
     8161 gtattttctc ctaagacgaa aaaagtggca tgcacgtttt tttcttttaa gattcgcaga
     8221 agctgcggtg ttgtggtcgc tcctggtcca tcatcaaagc tgattgcgac gcttttgaca
     8281 gccggatctc ggctcgtttt tgtactgtct tgtgttgatt gtgatgactg actttgtacc
     8341 ttttccgctg attcttgaat ggcttctttg acgttagctg tttcagttga ttggctttta
     8401 atggttgatt gttctgattg tttgaaagat tgtgacgaat gctccgaaga gtgtgtttgt
     8461 ccttttactt cggtgatcaa ggaggccacc gaagtaattt cgaaaaacaa aagcacggat
     8521 aaagcaataa tactgataac taaataattt cttttcacta tatctcttct ttctcttttt
     8581 ctcttcttat tatttacttg tacttacttg aaaattgaaa ctgctagtcg caactacttc
     8641 tcgtctaatg atccaaataa tcttccgatg ccaaaatcgt ttttgtcacg gacttaggaa
     8701 tgacaaattc tggtgtaccc atataaccag gagcaatttc gtattgagaa aaagtcaaaa
     8761 ctaattgatg ctctttgttc aaataaaaac ttcgtgtagg cgtaacaagt gttggttttt
     8821 ctatcgtccc ttcttgaata tcttgctcag tccaatagta cttagatgga tcttccttga
     8881 cttgttcggc catctgccgt ttgacttctt ttgtcaaaac agatagatag gtgtcatctt
     8941 tgaataaaag tggaagtgag agctggacac tgtttttctt gtcgattgtg tcgtatcttt
     9001 tttcaacatg gctatctgct gctgtttggg tgatctgcct ttcaatcacg agaaaccggt
     9061 tatcgtctac gattttctga taatctccca aaacttggta atgatccgtt tcaaaatttc
     9121 catattcttt ttcagctttt tcaaaatctg cttgtccttc gcgaaagtat ttttgattta
     9181 atccatgaat tgttttattt tctttctcat tttgtgtatt gatcttcggt acctgaatat
     9241 cgattttttc agatcgatca gtaaagcgtt cgcctaagat caattcaatg actgatccta
     9301 agatgggtaa atcttctgca aagcttcgaa cttgactatt aaaaagaagg ctacttgttg
     9361 taatcattac gatagccaaa gatactccga acccttggag acgtctcttt ctccgctttg
     9421 cttttataaa tgattgctct tcgataataa aacgctggcg gatctcctct ttaagccctt
     9481 tgggaattgc ttgttcttta taagttttct ttagtttttt taaattttct ttttgggaat
     9541 acatggcact cttctctcct tcattctagg atttgacgta aagcattcaa tgagcggtac
     9601 attttcgttt taactgtgtt ttcattcata tccaagatag tcgcaatttc tttcaaacga
     9661 aatccttcaa aaaagtagag gatcagaatc tctttttccg gactgtctat tttatctaaa
     9721 atcatatgga gctctgcaac ggaagtttct aaaggtaact gttgggggat atcttgaatt
     9781 tcaaataagt ccacaggcgt ccttttttgt ctgcgccaac tatctaatgc cgtgcggatc
     9841 aatatccgat aaaaccatgc actaaaatgc tcgatctctt tttctttttc aaaatagttc
     9901 agggctttat gaaaactatc ttgcatcaca tctaaggcct cttctttatc atgtacgtaa
     9961 ttcatcgtga tacggaacat tttttgataa tcagtttcga tgagtccatt cagcaggtga
    10021 cggtattttt cgatctgccg ttttttctta tatccagtaa aaatcattct ccactccttt
    10081 ctgcctcact taatatgacg tacaaggtgc gctaaaagtt tcaatttttt tattttttat
    10141 gtatttactt gggattttta tatacaaaaa agaaggtgtg acaaaaatcg tgtcacacct
    10201 ccttattctg ataggctgat cgtctctgtc acaacctctc gtaagaaaat aatgtttatt
    10261 ctttttcaga gtcagaaaat tcttcctctt cataatcttc atcatcatcg tcttcgtcta
    10321 tgatacttaa atcttcttcg atatttcctg gcaagtcttc ttcatcatct gtcgggtcat
    10381 ctgctccgat ttcttgcaag tcagaattat aagcagcgat ttcttcatct tcatcatcat
    10441 cgtcatcaaa cagatcatcg tcatcttctt ctgtcagatc tgtatcttct gggtcatcgt
    10501 cattgtaatc gatcgcgtct tcatcatcat tgataaaggc gttgactttc ttgcgtttac
    10561 ggcgacgtgg agtatcttca tcctcttcgt ccattccatg gtttacttct tcatcgatcg
    10621 aatcgatagc ataccatgaa cgcaagcccc aacgattatc ccctaatgag ataaagctgc
    10681 cgtcaatatt caagtcagta taaaattgtg cgagtgaatc acgaatttca ctgtctgatt
    10741 tcccaagata gttttggatc tggttagcta aatcagaaaa atccatgaca tcgccacgtt
    10801 gttctaagat cgcgtgtgct acttcgatca ttgataattc gcttttgttt gcaccttcaa
    10861 atacactaat ttccaatcag gacacgtcct ttcacagtct actccttatc atacaaaatc
    10921 agacacaaaa aatcaaactt ttcttttata tttacgctgt aaaatccaag gtcaaataat
    10981 attcgccgat tttttccggt cccatgaaca gatcataatc gattcgaacc ttccctgctg
    11041 tgggacggtc tttcaaacta acatgcagtt ctttcgtaaa agtagcaatc tggatcatac
    11101 cgtatggtgt tcgataggat gtttctaacc gttctttata gccaaatttc aaacgcatcc
    11161 gcatttctcc tgcacggatc agctgtacat gaccatctgg aactaatttg atcgttacag
    11221 gaatctcatc gcccgtctcc tcctgaatct ctttataacg tatgtacagc gtatcgccaa
    11281 ttgtcaccat ttgccctttc aaatcaaaat aaaaatcttg cacttcccct tgctgtttta
    11341 cttttgtacg caatttgatt gaaatcggtg ttccttgtga taattccatt attcattctc
    11401 ctttacttct catagctaca ttgttcctag tttcttttgt tttgtcaacc aacttgtcca
    11461 tttttacaga aaaccttttt tccaaaacaa ctctttgatt ttagagtata atattgtaga
    11521 aaatatgaat ttatacaaag gagccggttg aatgaaccct gcgattcgac tagatcagca
    11581 catttatcaa aaaaacgact tttccccttt tgcattaaca gatatattaa cggaatattc
    11641 taaagaacac cagcaaatct ttttacattt ctggcaatat gactgcacag tcatcttagg
    11701 tatgaaggat gttcgaactc cttttctaag tgacgggatc caaacactga aagatgcagg
    11761 ttacgctcca gtgatcagaa attcaggtgg attagggatc gtatcagatg aaggaatttt
    11821 gaatatttct ttgattttcc cccaaagtga cacaaaaaaa ataaccatag atgacgctta
    11881 cgagcaaatg ctcctattga ctaaatacgc ttttccaaag gcaccgatac aagcattcga
    11941 aatcacccat tcttattgtc ctggaactta tgatttaagc atccatggca aaaaatttgc
    12001 cggtatcgct cagcgtcgga tcaaaaatgg gatttctgtt atgatgtact taagtgtcaa
    12061 cggtaatcag gaaaaacgcg ggcaagtcat gaagcgattt tatcaatctg ctttaaaaga
    12121 agactttggt acaaatggct atcctgcagt ggatcctgct acaatggcaa cacttgaaga
    12181 actgcttgac caaccccttt ttgtaaaaga agtcaaagaa gcatttgcaa aagcttttga
    12241 aaagctgtat tctgcaagtg atttacagga ttctaccgac tggctaaaaa aagaaatcca
    12301 gctggagcaa tggaacaacc aaatccagag aatggaacaa cgtaataaca taaaggagct
    12361 acattatgac aaccccttat aaatatcaaa tgctgcctat ggaaaaagtt tttcgtgatc
    12421 cagtccataa ctatatccat gtccagcatc aagtaatctt agatttgatc aattcgaaag
    12481 aagtccaacg tctacgacgc attaaacaat taggcacttc ttctttcact tttcacggag
    12541 cagaacatag ccgttttact cattcattag gcgtttatga gatttcaaga cgaatctgcg
    12601 atatcttttc tcgaaatttt tcaaaagaaa aaataggcaa cggaggctgg gatgatcagg
    12661 aacggctggt gactttatgt gcggcactgt tgcatgatgt cggtcatggt ccttactctc
    12721 atacatttga acatatcttc catacgaatc atgaagccat cactgtagag atcatcactt
    12781 ctccagaaac agaagtgtat cagattctta accgagtaga agaaggtttt cctgaaaagg
    12841 tagctagtgt gatccaaaaa acttacccca acccacaagt tgtccaaatg atttctagtc
    12901 agatcgatgc agatcgaatg gactatttgc ttcgggatgc ttatttcact ggaacagaat
    12961 atggaacatt tgatcttacc cgaatattac gtgtgattcg tccttatgaa ggcgggatcg
    13021 ctttttccat gagtggcatg catgcagtgg aagattatat cgttagccga tatcaaatgt
    13081 atgtgcaagt gtacttccat cctgtttccc gtggtatgga agtaattttg gagcatctgc
    13141 ttcatcgcgc acacgagttg tatcaagaag atgccagcag ttttgaactc cattctcaat
    13201 tacttctgcc atttcttgac ggaagcttta ctttagaaga atacctcaaa cttgatgatg
    13261 gggtcttggg cacttacttt actcaatggt ctgaatctgc tgatcctatt ttaaatgatt
    13321 tagctaaacg ttttttgaat cgtaaaccac tgaaatctgc tactttttct ggcaaccggg
    13381 attcaaaatt agtccaagaa ctcactttgt tagtagaaaa ggtcggatat aatcctgttt
    13441 attacactgc tgtgaattct agctacgatc taccttatga tttttatcgc ccagaacaag
    13501 gcagacatcg cacacaaatc gagattttgc gaaatgacgg aactttgata gaattatcac
    13561 aggttagtca gcttgttgct gcattagcgg gacaagaaca aggagatgag cgatttttct
    13621 ttccaaaaga aatggttgat ccgtccttgc gtgatcatta tgatttattt gacgaaactt
    13681 atcaagaatt tgccagccat atccgtaatg gcgcattgat tgaaataaat taaaatatat
    13741 tggaggaaat tatgtctatc aaactagtcg ctatcgacat cgatggtact ttattaaatg
    13801 agaaaagaga agtaacaaaa gaagtaaaag aagcgattgc cgctgctgtt gcaaaaggtg
    13861 tttcgatcgt tttatgcact ggccgtccct taccaggtgt ccaggaacag ttaaacgagt
    13921 tgaacctttt tcaagataat gattacgtta ttacttataa cggggcactc gtacaacaaa
    13981 cgaaatcagg taaaatcatc gcccgtcacg gactgacaca tgaagatttc ctagagatcg
    14041 aagtgatggc acgccgtgtc ggcagtcatc tccattcgat cgatgacaaa gcgatctaca
    14101 cgcctaatcg agatatcagc gcttatacga tccacgaagc aagtttagtc aagatgccgc
    14161 taaaatatcg aacacctgaa gaaatgacac cagacatgaa catcgtcaaa atgatgatga
    14221 tcgacgaacc agaaatttta gatgcagcaa ttgcgcgttt accgcaaact ttccgtgata
    14281 aatacacaac ggtcaaaagt gcgccctatt acttcgaagt actaaataaa gaagcaagta
    14341 aaggtgcggc agttgcgaat ctcgcacagc accttggtat tgatcaagat gaaatcatgg
    14401 ctatcggaga taacgagaac gacttatcca tgattgaata tgctggtcta ggagtagcaa
    14461 tgggaaatgc cgtttctctt gtcaaagaag cagcaaatgt catcacttca tcaaacaacg
    14521 agcacggtgt agctgaagca atcaaaaaat atgtactgta ataaataaaa aaactgagag
    14581 aaagctgatc tactcgcttt ctttcagttt ttattttttt cgatccatct ctcgactgcc
    14641 catcggtggc taccacgttt caatttcaca agcgcttctt gagggctcac ccaccaaatt
    14701 ttattggtct tttcagttgg ttctcctata cgcttccaag aatcagcaac aaagaaatat
    14761 cctggattat aataataagt tgcacgataa ttcgagtaaa aatactcatc agcttctccc
    14821 agataagaag aaatttctgc cacaattccc atttcttcta tcagctcacg gacaatcgct
    14881 tcttctttcg tctcttctcc ttcaatttcc ccgccaggaa gaaaaaaagc accatttgga
    14941 gcctcgatta ctgcaatctg ctgatctctt tctatgacaa tatatgctgc ataacgggtt
    15001 tgataatcag caaattgctt tttttcacca aaaattggtt tatccatgaa tagtttccac
    15061 cttaacagtt ttattttcta tccattttac atgagaaatt tctcatgaac aagcacaaat
    15121 caactatttc cttacttctt tttgttatga ttgagtaaaa ggaaaaggag agatgaatct
    15181 atgaaaatgg ctcacacatg tgttcgagtg aaagatcttg aagcatcaat cgaattttat
    15241 caaaaggcat ttggttttga agaaagccgc cgccgtgatt ttccagaaaa caaatttaca
    15301 cttgtttatt tgacacttcc tggggatgac tatgaattag agttgactta caactatgat
    15361 catggtgcgt atgatctagg agacggctat ggtcatatcg ctatagcagc tgatgatcta
    15421 gaaaaattac acgagcaaca taaagctgct ggtcttgaga ttactgactt aaaaggatta
    15481 cctggcactg cgccttctta ctactttgtc attgaccctg atggttataa aatcgaagtc
    15541 atccgaggat agttagtatg gggcatttta ctcttgattg aataaaaaac aaacaccaat
    15601 aattaaaatg agacgcaaac ttttcattaa attgaaaaag tttgcgtctt tttttaacaa
    15661 ccattgtagc tataaggttg ccactctcca ccaatttatt tttaattcat ttgaaagaaa
    15721 tcaaataaac gaggtacctt tagaaaacaa aactgctttt cctcctatta tcacacgatc
    15781 ttcttttaat tcacattcta gatccccgcc ccttgatgac atttgataag ctgacaattt
    15841 tgttttattc attctttctg cccaataagg aataagatta gaatgtgctg atccacatac
    15901 tggatcttca ttgattgtta attttggaaa gaaggttcta gaaacaaagt ctttttgcac
    15961 actttctgct gtcacgatga ctcctattcc taattcaaaa tttttgatag ctgtaaaatc
    16021 tggctgcaat ttagccaccg ttttttcatc ttctaatacg aaaaataaat cacgtgctaa
    16081 gtaggcttct ttaatcttag ccccaatcgc ttcttcatat tcagacaata tcggcacttt
    16141 tttcggcata atactaggaa aatccatgta ataataatct ttaattcttg tcacaaatag
    16201 attaccgctt tgagtagaaa atttaatcgt ttcatcaggt attttgtaat agttaaataa
    16261 aacaaatgct gttgctaatg tcgcgtgacc acaaagatcg atttctcgat ctggcgtaaa
    16321 ccatctaagc tcaaattctt ggtttttctt cactgtaaat gctgtttcag ataaattgtt
    16381 ttcaatggca attttttgca tcgtaccttc tggcagccat tcttctagca cataaaccgc
    16441 tgctggattt cctttgaaca cctcatctgc aaatgcatca actacgtaat aatctaagtt
    16501 cattttaaag cccctttctc taatttgtta taaatatatt ctgcacaaca aagatcttca
    16561 atagctagac caactgcgtc gaaaatcgtc ggcctcgatt ctcctttata aaatgttttt
    16621 ttctcagaaa ctaattcgcc taatgtacca acaatacaag attcctctaa atctccttct
    16681 gccatggcta taagcaggtc cccgctttct gttaaggcag ctgagtaatc atctacatag
    16741 atttgagaag tttccatcaa cgatgtactt atctctcttg tatcaggagt aaatgtgccc
    16801 acagcattga tatgtgcgtc ttcccttacc cactcttttt gaagaaatgc ctctttacta
    16861 gaggtcaacg tacaaatgat ttccgctgga cgtactgcat cttctactga tattgcattt
    16921 ataaacgtta ttttaggata ctgtttttta gctttagcga taaaagtatt tcgtctttct
    16981 tctaaaatat cgtaaacaaa cactgtagtg atgggacgaa tggtagaaat cgcagtcaaa
    17041 tgcgacaatg cttgttgacc agctccaatt aaagcaagtg tttctgcatg cggatttgct
    17101 aagtaatctg ttgccagcgc agatacagca gctgttcgaa tccaggtgac tgcatttgcg
    17161 tttatcaatg ctttgggtag tccgttttga gcatcaaaca agaaaatctc tcctaaatga
    17221 gaaggtaaat gatactgatg attatccgga aaagcggtaa tgacttttgt gccaaaatat
    17281 ctgttctccc ccaaataagc aggcatcatc gcaaacatat ttttgccttt tccgtcaggt
    17341 aaaatttcca ccaatcgagg attttggctg attttaccac tttcataatc aaaaaaacat
    17401 tttttcatta cttgaatcgc ttcttcaaaa ctaaaagaag ctactacttc ctcaaaatta
    17461 ataattttca tagacaattc cctcctcaca aaagattaac ttctaaaatc aatacaatca
    17521 atggtaaaat tgtatggtat acaattttaa ggagtggaga aaatgcctat taattcttat
    17581 gacaattacc cgttaacttg gcgaccagac aaacaaaaat tgaagcgtcc ttattatcga
    17641 tcgttgattc aacaattaga acaagatatt ttaaagaata aattacagaa aaacactcag
    17701 ttaccctccc aaagagagct agctgattat ttagatttaa attttactac cgttggcaag
    17761 gcttataaat atggaattga gaaaggtttg ctgtatacga atattggaag tggcacattt
    17821 atttctccca atgtctttga gtccatcacc atctctacca atgaagtaac cgaacatatc
    17881 attgattttg gtctggttag ttcttttgaa gaatgtaata aattcgttgt cccgtttatt
    17941 caatcaacta gtaataaccc aaatcttgtt ggtttgctta attaccaaga tcccaaaggc
    18001 acaaattttc aattgactac tgcagcaaat tggttaaaaa ctcaaggagt cattccgtcc
    18061 ttagaatctc tatctattgt ttccggtgtt caaaatggat tggctatcac actttctgca
    18121 cttttctctc caggtgatag aatagcaaca gaccgatata catacgctaa tttcattgaa
    18181 ttggctcagt tatatcattt agagatcgtt ccaattgatt ttgacgagca aggaatgcga
    18241 cctgatatat tgattcagga atgtagaaaa aagaagattc atggtgtctt tcttatgcct
    18301 tcttgtaata atcctatagg gtttcaaatc tctcacaaaa gacgagagca attagccgaa
    18361 gtgattgaaa aagaaggtct atgggttatt gaagatgata tccattcatt tttaacaact
    18421 tactatcaac atacgatcat tcctcctttc caaaatttat tacctaacca aacaatctac
    18481 ttagctggaa tgacaaaatt tatttgttct ggattgcgag ttgcttatct tgtagttcca
    18541 gtacatgtcc aaagcaagat agaacaagca atttttaata taaatgtgaa aacttctgga
    18601 ttagatgtgg aagtcatcac tcaaatcctc cattcaaatg aagcaaatag gatcttagaa
    18661 actaaattaa gtcttacaaa aagagccaat cagctttttg attctctttt caaagtagaa
    18721 aaaccaacga atcgtgtccc ttactatcga attcttccag ttgatcctga gattgaccct
    18781 aagataattg agaatgactt tcttgctaac ggtatccgac tttatcattc cagacgcttt
    18841 actacacgta cgcaaccaga tgcattcgtt cgaatttccc tatcctctaa taaccttgaa
    18901 attttagctt ctggactaag tatcgtaaaa gaaaaaat
//
LOCUS       DADVXN010000053        18911 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.055, whole genome shotgun sequence.
ACCESSION   DADVXN010000053 DADVXN010000000
VERSION     DADVXN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.055"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(117..1166)
                     /gene="recA"
                     /locus_tag="KB942_001589"
     CDS             complement(117..1166)
                     /gene="recA"
                     /locus_tag="KB942_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBB6852015.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1275..2516)
                     /locus_tag="KB942_001590"
     CDS             complement(1275..2516)
                     /locus_tag="KB942_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBB6852016.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2756..3334)
                     /gene="pgsA"
                     /locus_tag="KB942_001591"
     CDS             complement(2756..3334)
                     /gene="pgsA"
                     /locus_tag="KB942_001591"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBB6852017.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3543..4466)
                     /locus_tag="KB942_001592"
     CDS             complement(3543..4466)
                     /locus_tag="KB942_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852018.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5205..6491)
                     /locus_tag="KB942_001593"
     CDS             complement(5205..6491)
                     /locus_tag="KB942_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6852019.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6484..7752)
                     /locus_tag="KB942_001594"
     CDS             complement(6484..7752)
                     /locus_tag="KB942_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6852020.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8004..8465
                     /gene="mscL"
                     /locus_tag="KB942_001595"
     CDS             8004..8465
                     /gene="mscL"
                     /locus_tag="KB942_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBB6852021.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8524..8970)
                     /locus_tag="KB942_001596"
     CDS             complement(8524..8970)
                     /locus_tag="KB942_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852022.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9063..9308)
                     /locus_tag="KB942_001597"
     CDS             complement(9063..9308)
                     /locus_tag="KB942_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBB6852023.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9423..10001)
                     /locus_tag="KB942_001598"
     CDS             complement(9423..10001)
                     /locus_tag="KB942_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6852024.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10078..10608)
                     /locus_tag="KB942_001599"
     CDS             complement(10078..10608)
                     /locus_tag="KB942_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBB6852025.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10612..11472)
                     /gene="rlmB"
                     /locus_tag="KB942_001600"
     CDS             complement(10612..11472)
                     /gene="rlmB"
                     /locus_tag="KB942_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBB6852026.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11465..11860)
                     /locus_tag="KB942_001601"
     CDS             complement(11465..11860)
                     /locus_tag="KB942_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBB6852027.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11857..13269)
                     /locus_tag="KB942_001602"
     CDS             complement(11857..13269)
                     /locus_tag="KB942_001602"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBB6852028.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13266..13802)
                     /gene="cysE"
                     /locus_tag="KB942_001603"
     CDS             complement(13266..13802)
                     /gene="cysE"
                     /locus_tag="KB942_001603"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBB6852029.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13888..14127)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14124..15584)
                     /locus_tag="KB942_001604"
     CDS             complement(14124..15584)
                     /locus_tag="KB942_001604"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBB6852030.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15754..16911)
                     /locus_tag="KB942_001605"
     CDS             complement(15754..16911)
                     /locus_tag="KB942_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBB6852031.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16945..18315)
                     /gene="radA"
                     /locus_tag="KB942_001606"
     CDS             complement(16945..18315)
                     /gene="radA"
                     /locus_tag="KB942_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBB6852032.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18378..18905)
                     /locus_tag="KB942_001607"
     CDS             complement(18378..18905)
                     /locus_tag="KB942_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBB6852033.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4999 a         4172 c         3392 g         6348 t
ORIGIN      
        1 ttttatgtac ccactcgtat aattaaataa ctcacgattt ctttattcaa taaaaaaacg
       61 gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca taatttttat
      121 tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc gacttcttca
      181 ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc aggatgatta
      241 gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt ataagaatac
      301 caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag ttctccttct
      361 tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg aggtgctact
      421 ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat
      481 tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc gcgtcctcca
      541 ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg gttgatgaaa
      601 atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc
      661 aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga
      721 actaaagcag caactgaatc gactactacg atatctacag ccccacttga tactaaagca
      781 tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata
      841 ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc aatgaaagcg
      901 gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt tgttttacct
      961 gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa
     1021 gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc gattttttcg
     1081 cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag tgcagcatct
     1141 aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac aaatcagagc
     1201 aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca
     1261 aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa tccttgcata
     1321 accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc cgaaaccacg
     1381 ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc taacgtgtcc
     1441 ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc acgtgcttga
     1501 atggccatct gctccacaca ttctttgctg actgttccat attctgctaa tagctccgga
     1561 tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc gcctggaaat
     1621 acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt
     1681 tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac ttttgctagc
     1741 gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat tttttcttct
     1801 aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt cagtcgtaat
     1861 gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt ctcaatcaga
     1921 tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa aactttagaa
     1981 attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg ttccacaaac
     2041 attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt atctgtttga
     2101 taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc ttcgataatc
     2161 tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa atatgccaat
     2221 aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc cgcagttgct
     2281 tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat gagttcactt
     2341 cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc tacaactgtt
     2401 tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt ggcattcgta
     2461 ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc tttcattact
     2521 tcccgctccc ttatatagta tacgctgtat ttttatattt ttttatttaa aaagagggca
     2581 aggagctgtg acaaaaatct tgtcacagct ccttgttcca aataaacagt gttctcctgc
     2641 caaaacctcg gtcataagtc acaatataca taaaacatga aaagctgttt tttgaaatta
     2701 tcccttgtga ctcagagtta gacggcaggc tcacaacctc tccactaaac gatacttaca
     2761 ttgatccttt gaacacaccg ctgtttttga tgaagtaatc cacacctgaa tagatagtaa
     2821 agatcagaca agcataaagc atgatttgat ctactggaat accgatcagg ctgaatggga
     2881 tattattgat aaacagcaag ataatagcaa ccatttgtgt agccgtcttg acttttcccg
     2941 gccaagctgc agccatgact tctccatgtt cgacaagtag taaacgcaag cctgtgacgg
     3001 ctaattcacg acagacaatg atcgctacta cccaaccagg ggcctttcct tggtcaacca
     3061 acatgataaa agcagtcata actagcattt tatctgccaa cgggtccgca aacttgccaa
     3121 aattagtgac aaggcctcgc gcacgggcaa tttttccatc taaccagtct gtgatactag
     3181 ctaaagcaaa aataactgca cctactagct gtgtgacagc cagtgtggtg tctcctacgt
     3241 taaattgtcc ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa
     3301 ttctaagtac agttaattta tttggtaaat tcacactatc gactcctttc ttccctattc
     3361 tacgtgacta tcagcgaagt ttccaccgat attatttact tttccaaatg aatttattca
     3421 aaggagtatt tttattgaaa ttgatctttt tgaattaacg aagaaggacc gcgacacaga
     3481 tttgtcacga tccttctttt ttatacctac aagtaaacag atgcgtaaac taatttatac
     3541 gatcattctg cgtattgaag agtaaggttc acattttttg gactctgatt gttttcccct
     3601 ggattaactg gtacggattc tccattcgct ttgatcgttg cattgcttgc tgcaccaatc
     3661 gtgatggtcg catttgttgc tgtttccggt aacacggtac tctgtgtttc atttgctgcc
     3721 agtgtccctt gataaacata ggcattgtct actagcacac ctaaccatac acgattgaca
     3781 gcagtaaatt caagggttac aggcttggta gcgtctgtga tatttactgc gatggcactg
     3841 ccagaatctt ctcctgtagc tattgccatt ttcttttctt cacttgattt tgttgttgat
     3901 tctactgtag aagactcttt ggtctgcgtg ctttgctctg aggaagagga ctcctcgact
     3961 gatccttcta ctttaagcga agaagctgtg tctccgatta ttggatcagc ttgacgatct
     4021 tgccaagtca tatagcccac gacaacgaca atcgccaaag caaccaatcc taataaaatg
     4081 acaggaagac ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat tgcttttcta
     4141 gaagtactta ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc
     4201 ccatcaaaaa catcgactaa atggtctcca tcttctccta cagcttcagc gtattgccga
     4261 ataaatgccc gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt gtcgattgct
     4321 tccaaataac gtttttgaat ttttgtaatc tgctgtaatt catctaaaga tattttttta
     4381 ttcaggcgtg cttgccgtaa tttttctccg atggtttcaa aagccactcg ttcatcttct
     4441 ccagtctgtt tgattctccg aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg
     4501 ctgtgacaaa agtcatgtca cagctccttt tttccgaata aacattgttc tcctgccaac
     4561 tcctcggtca caagtcacaa tatccaataa atataagaag ctgttttttg aacttgttcc
     4621 ttgtgactcg gagttaaacg gcaggctcac gacctctttc ttaaacggtg ttcaatcagc
     4681 tgttcctcgg tattagctca aaaaccggca aaatataagg agatattttt ccgtatgttt
     4741 ctctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tgttcaaaag
     4801 cttgcatctg cggtaataag tccaaccaag ttaaaaacat taagaagctg tttttacttg
     4861 gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttggtac acggtgttcc
     4921 agaagtagct tcttcggcaa taagccgaaa tatcacaaaa atttgagaag caattttcgt
     4981 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctttg attacttcgt
     5041 ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta ttttctcact
     5101 aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg ttcctactac
     5161 aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt tggtctcatg
     5221 taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag tacgtctttc
     5281 atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt
     5341 tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata ttgtccaagc
     5401 attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc agcgaatcca
     5461 agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc tccgctgaag
     5521 tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc atcgataatt
     5581 ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat ttggaacaac
     5641 agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat
     5701 cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc actttgtttg
     5761 atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg agggaaattt
     5821 tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat cttaccaact
     5881 acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata caagtcttga
     5941 gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta
     6001 ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc ttcatacatt
     6061 tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt gaagtaaggt
     6121 gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac
     6181 agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg tttcccaaat
     6241 aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa atgggcaatt
     6301 ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt atcgatcgaa
     6361 ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg caacaaatac
     6421 acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg ctcatattct
     6481 gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc
     6541 agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc attcttcatc
     6601 agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg
     6661 agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag acagttccaa
     6721 ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat gaagtacttg
     6781 atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga acgtatccac
     6841 gctactagag gcataataag ctagactttc tttctctctg acattcatga aaagtttaga
     6901 gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc
     6961 gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg gctcacgtac
     7021 ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg gataaactgt
     7081 ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg gatcaacatc
     7141 gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt aggacgctag
     7201 cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga tcttttgttc
     7261 cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc
     7321 cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg tcaactgatc
     7381 aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat ctaccgcttg
     7441 agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc ctgcgtttag
     7501 ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat aaagctctgc
     7561 aagttgcgca cttaactttg tctgatccga ataattcaaa ctatttgttt ccaacatgct
     7621 gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa
     7681 aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc cttctgccaa
     7741 ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac tcttttaatg
     7801 ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca ggcaattcgc
     7861 cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt atcaaaagaa
     7921 acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat actaaagaca
     7981 aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc atgcgtggag
     8041 atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc
     8101 aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg atcacaggta
     8161 cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat
     8221 ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt tttgtcttat
     8281 tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg
     8341 aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat cttcttgcca
     8401 aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc gatacttcta
     8461 aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga
     8521 ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata
     8581 cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc
     8641 gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac
     8701 aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg
     8761 gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg
     8821 aaaaaacgta tgccaagctt ctccatcaat gtcatcaact tcttgataag gacactgttg
     8881 ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa
     8941 ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt
     9001 agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct
     9061 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
     9121 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
     9181 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
     9241 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
     9301 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
     9361 taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa gatttttcta
     9421 aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac tctcccgtaa
     9481 gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac aaaatcaacg
     9541 ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat ttcgcaattt
     9601 tcgattgctt cttttaataa aagatgctga attgctaaag gttcttgcaa tgtgatctct
     9661 gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc atctttttga
     9721 tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt gcttataacg
     9781 cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct caatgcctga
     9841 tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct taaataatag
     9901 gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc aaatgcttct
     9961 tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt cctccataaa
    10021 ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa cgatatgtta
    10081 tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact gctcttcatt
    10141 ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt gggctattgt
    10201 tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta atgctccttg
    10261 ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg
    10321 gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt cttctttcgt
    10381 aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa ctagttgggc
    10441 atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata aacgatgtaa
    10501 aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa gttccggcca
    10561 cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg tctactcccc
    10621 ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg catttaagct
    10681 ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt tcaatcctgg
    10741 actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg cattccattt
    10801 ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt ctttgagctg
    10861 ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt
    10921 ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta tgatcacacc
    10981 atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat
    11041 gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt caatcagttg
    11101 gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat gcacgccgtg
    11161 atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag cttgttcacg
    11221 tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt
    11281 attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc
    11341 ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt gctttttatt
    11401 tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct
    11461 ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat tcttccatcc
    11521 gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc
    11581 gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt cttcctctgc
    11641 gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa
    11701 aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta ttgggttttg
    11761 tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca tctccaacat
    11821 aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc tccatcgggt
    11881 tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac gaatttcgtc
    11941 gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca attgttcaat
    12001 ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa gccattgcga
    12061 gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt ctagataagt
    12121 gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat taaaatcatc
    12181 gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt tatccgcatc
    12241 atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa acgtattctt
    12301 cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg
    12361 acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt ttttgatcat
    12421 ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt cttcgccaat
    12481 cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt tcgcttcact
    12541 ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac catggatatc
    12601 gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc aaccgggacg
    12661 tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt tccataaagc
    12721 gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac ttgctccgac
    12781 ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt tacgggtacg
    12841 gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca cttgaataaa
    12901 tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag gttcgacatt
    12961 caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt ctggtgctgt
    13021 aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga aattcgatac
    13081 gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga aagcaatggt
    13141 gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga catacatacc
    13201 gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg tattgtagat
    13261 ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt tgctggtact
    13321 cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc accgattttg
    13381 gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag catggctcct
    13441 tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa tgttacgcca
    13501 tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat tcccattcca
    13561 tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac accagttagg
    13621 aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg atataaaaaa
    13681 tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac gatctcagct
    13741 gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc
    13801 atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt ataaagaaaa
    13861 gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt tccacttttc
    13921 ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg gctactttgt
    13981 ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc cagaggcatt
    14041 tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat gatcagctgc
    14101 ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc taatgatttt
    14161 tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg tcctgataca
    14221 gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt ttgaacagct
    14281 ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag ctgtgctttg
    14341 aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc tgcttcattc
    14401 aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc agcatagctc
    14461 atttgaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt ttcatctggg
    14521 ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt aaatgcatct
    14581 agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc atcgaaagct
    14641 gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg gtgttcgaag
    14701 atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa cattgcatct
    14761 ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc gtcacgtttg
    14821 cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa ggttggtggt
    14881 gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat atggtcatct
    14941 ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc aaaattataa
    15001 gttggcatac catcgcgttt ttggatgaca aagtcgccac cgatattatc ggattcgaac
    15061 gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg cacacggaaa
    15121 cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc
    15181 gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc ccgttctgct
    15241 tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag cagctgatca
    15301 atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta ttctccagga
    15361 ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa ctggcttttt
    15421 tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat aaagtctcca
    15481 tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc aatatgcagg
    15541 tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt cctcttcttt
    15601 caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca aatatcattt
    15661 atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa gccaactggt
    15721 tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag tctttttctc
    15781 tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg
    15841 tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat tcatgtaatg
    15901 ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc cttgttggcg
    15961 ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga cgactggttt
    16021 tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt cagagacttt
    16081 attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca atttgatcaa
    16141 tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga cagatatacc
    16201 ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca cacgtttcaa
    16261 actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa
    16321 ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa tgatcacgct
    16381 tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta acagttgact
    16441 ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa aaactttctt
    16501 ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga tgatcatcag
    16561 taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag gggtcgagat
    16621 cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa gataagtcaa
    16681 gctgacctca cttagttttt gttctacttg tttgataata gctgagatat gtttggccaa
    16741 cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa atgtattatt
    16801 cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa atgaaatacc
    16861 caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc
    16921 tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt cagccaaagt
    16981 tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt tgtttttagg
    17041 taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt caatatgact
    17101 gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg ttggctgtgt
    17161 tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa tcgctggttc
    17221 atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa gaagcaaccc
    17281 agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa gacctgttgt
    17341 tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct ctaccaaaat
    17401 tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt ctaggcgttc
    17461 ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat gcatttcaaa
    17521 aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa tccgaaaact
    17581 atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta acattcttgg
    17641 gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta tcccatttgt
    17701 tttagcggtt ttgagtagtt ctgcagtggt ttctcttact tggctgacac ttcctgccac
    17761 acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat aatcagggga
    17821 gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata gataaaattc
    17881 tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc tttcttcgcc
    17941 ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt gtaacagcaa
    18001 agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg gcaccacacc
    18061 tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt cttttttagg
    18121 gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg tccgagttct
    18181 gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc cgcaatttgg
    18241 gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa attgtacttt
    18301 tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt aaatcaagct
    18361 taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg ttgagcagtc
    18421 gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat acgctcgcct
    18481 tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat atggccttca
    18541 tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt caacactaaa
    18601 aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc ttctcccatg
    18661 taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt ttctgataca
    18721 tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc tttccagata
    18781 cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg atgtgtagcg
    18841 cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa tcctcttttt
    18901 ctcaccggtt t
//
LOCUS       DADVXN010000054        18876 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.056, whole genome shotgun sequence.
ACCESSION   DADVXN010000054 DADVXN010000000
VERSION     DADVXN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.056"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            167..1594
                     /locus_tag="KB942_001608"
     CDS             167..1594
                     /locus_tag="KB942_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852034.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1686..2051)
                     /locus_tag="KB942_001609"
     CDS             complement(1686..2051)
                     /locus_tag="KB942_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6852035.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2048..2413)
                     /locus_tag="KB942_001610"
     CDS             complement(2048..2413)
                     /locus_tag="KB942_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6852036.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2605..2853)
                     /locus_tag="KB942_001611"
     CDS             complement(2605..2853)
                     /locus_tag="KB942_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852037.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2852..2980
                     /locus_tag="KB942_001612"
     CDS             2852..2980
                     /locus_tag="KB942_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBB6852038.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3037..4731
                     /locus_tag="KB942_001613"
     CDS             3037..4731
                     /locus_tag="KB942_001613"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBB6852039.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4735..4854
                     /locus_tag="KB942_001614"
                     /pseudo
     CDS             4735..4854
                     /locus_tag="KB942_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4831..5004
                     /locus_tag="KB942_001615"
     CDS             4831..5004
                     /locus_tag="KB942_001615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852040.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5382..6515
                     /locus_tag="KB942_001616"
     CDS             5382..6515
                     /locus_tag="KB942_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBB6852041.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6546..6866
                     /locus_tag="KB942_001617"
     CDS             6546..6866
                     /locus_tag="KB942_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBB6852042.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6895..7359)
                     /locus_tag="KB942_001618"
     CDS             complement(6895..7359)
                     /locus_tag="KB942_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852043.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7491..8744)
                     /locus_tag="KB942_001619"
     CDS             complement(7491..8744)
                     /locus_tag="KB942_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852044.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8773..9090)
                     /locus_tag="KB942_001620"
     CDS             complement(8773..9090)
                     /locus_tag="KB942_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6852045.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9116..9436)
                     /locus_tag="KB942_001621"
     CDS             complement(9116..9436)
                     /locus_tag="KB942_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852046.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9900..11315)
                     /locus_tag="KB942_001622"
     CDS             complement(9900..11315)
                     /locus_tag="KB942_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6852047.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11332..12648)
                     /locus_tag="KB942_001623"
     CDS             complement(11332..12648)
                     /locus_tag="KB942_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852048.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12798..13496)
                     /locus_tag="KB942_001624"
     CDS             complement(12798..13496)
                     /locus_tag="KB942_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBB6852049.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13785..14741)
                     /locus_tag="KB942_001625"
     CDS             complement(13785..14741)
                     /locus_tag="KB942_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6852050.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14854..15687)
                     /gene="msrA"
                     /locus_tag="KB942_001626"
     CDS             complement(14854..15687)
                     /gene="msrA"
                     /locus_tag="KB942_001626"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6852051.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15862..17496)
                     /locus_tag="KB942_001627"
     CDS             complement(15862..17496)
                     /locus_tag="KB942_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852052.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17483..18868)
                     /locus_tag="KB942_001628"
     CDS             complement(17483..18868)
                     /locus_tag="KB942_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852053.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT         5975 a         3714 c         3146 g         6041 t
ORIGIN      
        1 aatttatttt ttaatagaat tatctgtttt ccaaatttta taacataaaa aacctttttt
       61 aacggcattt atatgctttt tcagaaatta actgatgatt tcaaaaagaa aacatgtcat
      121 aatatttccc atattgtgca aaaaaataca aggcggaggc tctactatga ttgaagaata
      181 tattgagaaa aatattttaa gacagctgtt tttatgtggg caattctatg taaataaaga
      241 agtcaatcta gaaaagctct ccaatctcct gcatgtttgc aaaaccactt tactcaatga
      301 tatcaataac ataaaaaaag aatttgaaga acagatcgct tatacacatc gggaaaagga
      361 ccgttacact ttatattttt cagaacatat acctcgatgc aagatcatgc agcaattgtc
      421 tcaaaattcg ttgtttttaa aaacttgtct gctttatttg gaagaagatg aacctgatta
      481 tcttcaatta actgaatgtg aattcatctc tgtcagcaaa gcttattcat taaaaaaaca
      541 agtattagct tattttaatg actgcgggat cgaaatcgac cgctactccc ctcgattcac
      601 tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg gttttaattc
      661 ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat ccgtaacgga
      721 aaatagtggc agattttatg ataaagaaaa taaagaaatc ttatcgattg gttttgctat
      781 cagtttccta cgccagcaag tgtgtgctgt gacaatagat tcaaaattta tcgaagaaat
      841 caaaaaacgt cctatttaca attatgtcga atcagcatgg gaaaacactg atttccaaac
      901 ctattataaa aaagaagaat tcgcctttat tttgacacta ttcaatcttt gtaattatgg
      961 tttccattct tatcagttga ttgcggaaga ctttcaacag ctacaccaag tgtttattga
     1021 caatactccc gaaataaaag aactagttgc tacctttgaa tctcacttca atcaagaatt
     1081 attcggaaac caaccgttcg aacgtgcgct gattcatctg atgcgttcgg cttgggataa
     1141 ctatcaatta ttcatgccag aaaaatttta tcttttaaat gaggaacaga caaatttata
     1201 taaagaagtc cagacaattt tttccagctg gtccagccag ttaccttatg atctacggtt
     1261 gaatcctaat tgcatgcggg cttttgtgat cgaattgagc ggaattctcc gtctgacaaa
     1321 agaacatctg actatctata tcgtcacaaa ttcggatgtt cattatctga tttatcggga
     1381 agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaattt attcaagtat
     1441 atctgatata aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg aacgtacatt
     1501 atatacacca gatgcggtcc aatatgaaaa tattattcct atttcgatca atacgatcga
     1561 tcgagctatc atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa tcatgtcaca
     1621 gcctcttatt tgttgaagat gcccatttct gtaacaatct cttgatacac gtcaggctca
     1681 caacctcact tattttggac atctacgggt ccgatctctt tatttaaaaa ataagaaatg
     1741 atggttcgga taactaccaa cgccgctagt tggaaaatat cctgcaaggt aggtttgata
     1801 atcgattcga tgatcccagc tgaaacaaga atttccaaac ttagaagtat atagcctccc
     1861 aacatctttt taatagcatt gttctcgctg actcggtcgc tttgcacttt tgtatggaca
     1921 aataaatatt tcaaaaaatc cttgattgat agacaaacac cccaaaccaa taaacagata
     1981 gcaaagatat caagtcccag tacaagtttt tcgaaatatg gaagaagaaa ctgcatcaac
     2041 tctgtcgtca tcctacctct cctcttcttt gtttttcttt gtcttctatt tcctgattca
     2101 agaaataaga aatgactgtt cgaatgataa taataagtgc aagcttcaaa atatctgtcc
     2161 aagtcggctt gataactgac tcgatgatat cagcaacgac tagtacttcc aagcttaaca
     2221 aaatataact tgctagcagt tttcttgtat ctgcattacg agtattacgt tctgcatagt
     2281 ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc ccgacaatta
     2341 agataccgat agaacaaata tttaaaatag caatgatcca ttctagaaat tcaattaaat
     2401 ttgagcgaaa catatacgct acctactttc ttttaatttg ttcctattct tttattgtaa
     2461 aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa tgataacggt tcgcttaaat
     2521 ttttcttttt cactctccca tttttctccc tccatccata aatcgtggaa gtatccaaaa
     2581 caacgtagca agttaaaata aggcttagtc cgtcatcagc ttttcaaaga agcactgtct
     2641 cggtgctttt tttgtaccca aatttctaaa aatagaacca tacacctcct caaatctttt
     2701 cttcttacct accctatttt tatttacagg aaaatcagac acttcttctt ttttgtcaaa
     2761 atttcctttt tcttctcgtc ctatcatttg gcattctaat aaaaacgaac tatccttgga
     2821 tatagaaaga gaaaacaagg aggcgttttt catgagtaaa aaattaatca aagtcggcat
     2881 cggtttaggt ttgcttgccc ttggcgctgc atatctagga aaaaagacag gactattcga
     2941 agatgacagt catttatatg atgaattcga atcaatctag catcctattt agttgaatca
     3001 ctacggacat acttggaaga cattggagga atcacaatga gaaaaagtaa agcaataatg
     3061 attggtgccg gtattgccaa tatggcagcg gctgtttatt tgattcaaga aggcaaatgg
     3121 aaaggggatc agatcacctt ttattcattg gatgatcacg gttcaaatga tggtgcaccg
     3181 accgacactg tagtggatga atattggaat aaaaaccatc cattagaaaa ccaaaaaggc
     3241 tatattgctc gaggcggtcg catgcttaat taccgtacct atgtagattt gatggatctg
     3301 ttaagtcgaa taccttctgt tactgagcca gggatgactg ctgaagaaga tacgagagat
     3361 tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg caaaggaatc
     3421 attgatggcg gaaaactggg attcaataat aaagaccgta ttcttctgac taaactgatt
     3481 gctatgcctg attcagaaga agaaaaacta gacaatatca ctatcgaaga atatttcaaa
     3541 gatgatccac acttttttga aacaaacttc tggtttatgt gggaaacaac atttgctttc
     3601 agaacacgta gttctgctca agaattgcgt cgctatatgc atcaaatgat ttatgaattc
     3661 actcaaatcg aacatctagt gggcgtcaat cgaacacgtt acaatcaata cgaaagtatt
     3721 atgcttcctt tgatcgagta cttgaaagaa caaggatgca aaatcatttt gaaccgccgt
     3781 gtcactgact gggaattcaa agaaacaccg atgcaagatg aaatcactgt gactggtttg
     3841 aaaattacca atacactgac tcaagaagaa gaatacgttg tggtcgatga agatacagca
     3901 gttattttca ctaatgggtc cattacagat tcagctactt taggagatta cgaaacacca
     3961 gctgtagaaa atatggatta tggtgcagct tctagtctct ggaaaaaagc cagtgaacgt
     4021 ttctacaact tgggaaatcc tgacaaattc tttgctgatc gagatgcaag cgaatgggtc
     4081 agctttaccc tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg tatcacgact
     4141 caagaaccag gaaatgcctt gaattcattt atttcaacca caccgatcac gccgttaggc
     4201 caaaaagatg tcaatatgtc tattgtcgtg catcatcaac cgcattttac gtctcagaaa
     4261 ccaaatgaaa cagttatttg gggttatttc ctttatccaa gacgacgtgg cgaatttgtg
     4321 gataaacagt atattaaaat gaatggaaaa gaaatgctag aagaactgat tggacaactt
     4381 tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag aaacagaaat tttcgacagc
     4441 gtaataaata atatccctgt ctatatgcct tatgcttccg cgttgttcaa taatcgtgcc
     4501 aaaagcgatc gaccaaaagt cattcctaaa cattcgacca atctggcctt tacaggagaa
     4561 tttgtcgagc agccttatca gatgatcttt actgaacaaa gtgctgtacg ttctggagaa
     4621 atcgctgctt tccattttgc gggtattcct atgtcccgtc ttgtcaaaac accgcgttac
     4681 gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata atcaagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca ggctaaaaca gcttcttatg
     4801 tttttgattt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
     4861 ggttatgaaa aagctgccct gcatcaagta ataagagcag ccaagctaaa acagcttctt
     4921 atgtttttga cttggttaga cttattaccg cagatgcaag cttttgaaca ccgtttacca
     4981 agaggttatg aaaaaggcgt ttagtttcga gtatgaagaa aaattttgtg aaaatggttc
     5041 ctcacgtttt tgcataaatt tgacttaata ccgagaaact gatcccttct gtcataacct
     5101 ctttttattt atgtctgttt ctttttgaaa acaactattt tttcaacgtt tactctcttg
     5161 ccaatccaac atcatctaac ggatacaact atttcaatga ttaccattac actaaaaaat
     5221 cggaaacaca cctctccgaa cacaaataca taagactata gaatgtttca tttaagaaaa
     5281 aaagcgtttc attatcaaaa aggtaagatc tcctttaaaa tgaaattatc tgtagtacta
     5341 ataatgcaga caattttatg aatctgaaag gagcatctcc aatgaaagca gtcacttggc
     5401 aaggaaaaca gaaaatggaa atcagaaacg tagatgatcc aaaaatatta gaaccaacag
     5461 atgcaatcat ccgtatcaca gcaacagcca tttgcggctc cgatctgcat ctttaccatc
     5521 atggtgaagc agttttggaa aaagggtatg ttgtaggaca cgaaccaatg ggaatcgttg
     5581 aagaagtggg atctcaagtt caaacattga aaaaagggca acgtgtggtt atccctttta
     5641 atattagttg tggtcattgt catttctgcc tgaatcacat ggaaagccaa tgtgatgaat
     5701 caaatccaga tcagttatat ggcgggcttt tcggatttgg caagttgaac ggtaatcatt
     5761 ggggtggtca agcggaatat ctaagagttc cttatgcaga ctctacttca tttgttgtac
     5821 cagataatga tcttcctgat gaaaaagttt tatttctatc agatattttg cccacagctt
     5881 attggagcgt tgaaaacgca ggagtaaaaa aaggcgatac agttgtgatt ctaggttctg
     5941 gtccagtcgg tctattcgct caaaaattcg ctgttatggc tggagcagag cgcgtaatcg
     6001 cagtagatcc agtacagcac cgtctagata aagcggctaa ttacaatggg gttgaaactt
     6061 atttcttaga agacacggca caagctggaa cagaattgta tgaactggct aaaggtggcg
     6121 cagatgtcat tatcgattgt gtaggaatgg acggattaga gcctgtcaaa gaaaaagcga
     6181 aaaatttagt aagcttacaa agcggcacaa tctctccatt acagatggca tcgcaagctg
     6241 tcaaaaaatt tggaacagtc caagtaactg gtgtttatat gactccagct tcttcttacc
     6301 cgctgcaaga attttttatg cgggatatcg ctgtcaagca tggacaggca ccaattatcc
     6361 atctaatgcc aaaaatttac gcaatgattg cagaaggact attcgatcct acacaaatca
     6421 ttactcattc gctgccacta tcagaagcag cacaagcata tgagatcttt gacaaaaaag
     6481 aagataaaaa tataaaagtt gttttgaaac cttgaattca gtaaaaaata caggtgattt
     6541 tctttatgcc caaaatcaaa cgaaataccc atgagatcga aataggaaaa gaagaagata
     6601 tcgaaatacg aggacgtcgc ttccgcttct atttccaaaa tcgttacaca ttgatctcac
     6661 tggctgtcga tttattaacc gggattttct atatttttgg aagccttgct tccttaacta
     6721 aaatccccga tcgatatggc acctattttt atttggctgg tgctattttc ttgacgattc
     6781 gtcctattct cagaatcgta aggaacattt ttatttacga cgaaaaaaaa gcaatgaaga
     6841 gaacaagcaa aaaaaaagag gcatgagaat ttttcaaatc tcatgcctct tttttcaccc
     6901 tatcgtatca ctttgataca gacgagcggt cgtcccactt tcttccttga ccattgtcaa
     6961 aaatgtccac tcgtatcgtt cataaaacgt ggtatgatct gtaattaagt aaagtttttc
     7021 gtacccaagt tgatgcattt cttttctgat ttccgctaat atccgcttgg ctatcccctg
     7081 cttacgcttt tccggttcaa caaacagggc acaaagattt ggtgtcagat cagggcggtt
     7141 atggaagtcg ttttcaatga cgcccgctcc tccgacgatg gtttcttttt catctaagac
     7201 cacataccat tgaggaatac tttccttatt agcgattcca gtacgaatac tttcctcata
     7261 cacaacagca ggaactcccc attttgatga aaaccaattc gcgctttgtt ttactaattc
     7321 tgggtgttct cttaatttga tccagttata tgtaaccaat gtttaaacct ccgactaaaa
     7381 taaaacgttt acaaaaatta tacaagtctt tatgaaaatt agcaatgaca acaatagtga
     7441 tgtaaaaaaa aaaacggaaa acaatcactt gttctccgtt ttttgattaa tcaagcatct
     7501 aaagcttctt ttcttctttc ttggatcgta gctaccttga cgaacggtag atagatcaaa
     7561 atagaaagaa ccaaattgac agcggctagt acaccacctg caatactttt tgttgccaat
     7621 acaccgccaa taattggtgg agttacccat ggaggcataa cggtagctgc tggcactaaa
     7681 cctgtagaag tcgcaaagta agcaacacta actaagacca ttggtgtcaa aataaatgga
     7741 atgaacatga tcgggttcaa cacgataggt aacccaaaca tcgttggttc attaatattg
     7801 aagacacctg gcgcaatcga caagtttgtt acaaccatat atggtttgtt tttacgtcct
     7861 actaaataaa tagctaacag taaacctaaa gttgcacctg ttcctcctaa gttgacaaag
     7921 ctgtcaaaga aaggtttgtt cacgataaac ggtgcgctat gtccagctgc gattgcatta
     7981 acgtttgctt caatggctgg cgcattgatt gtttgcatca aaggatcgac catattcgct
     8041 ccgtgtaaac caaagaacca taagaatggt gtgataaatg caagcagtaa ggcagatgga
     8101 taagagtttg caagtcccat aaatggttct tggaccactg tatagaaaga acctacgata
     8161 tcatcaacac cgaatgcagc aaaaattgca gcaactaaag caaacgcaga gatagtgatc
     8221 attgctggta gtaatgaagc aaatgctttt gcaactgccg gtggcacacc atcaggcatt
     8281 ttgatgatca atttttcatt tccgcttaaa cgtacaaaga tttctgttga aataatccca
     8341 acaataatag caataaacaa accgtttgag ctcataccta atgctccgcc taaagcgaag
     8401 aaagagccta aagaaatcac acctgcggaa acaccgtctt ttcggtagcc gttagctaaa
     8461 tgataagcaa gcaagaaagc aatcaagaca gaaagaatgg caaatgtacc gttccaagca
     8521 gcagccccga atcctttcca tgattcacca ccaaaaattg agttcatcaa ttcttggaac
     8581 ccgggaatcg gtaagttatt gatcattact gctaatgctc ctaagatcat taacggcata
     8641 ctcaccataa atgagtcccg aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct
     8701 acaggtataa aatgtttctc cataaatgca acaaatttat ccataaatct tccctctttt
     8761 ctttactgta gattacgcgt tcaatcgacg atacagatca atgatctctt ttgctaaatc
     8821 tgtaaaagct atagatgtca tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc
     8881 aacgtgattt ccttgtgctt cttgtgtcag catctctgtt tgagagtgat gtgcttggac
     8941 taaagattcc tctgcatctt tgattttttc atctgctaaa gtgaaatcac cttttttcgc
     9001 agcagcaatc gcttccattg catcgctttt tgcatttccg ccaaacatga tcagacccat
     9061 gatggcttct agattttttt gatcttccat ttattgtatc ctccgataat ttttattatt
     9121 tgtttattag tttttctgct tggtcgagta ctttttcacc attcatcatg ccatagtcag
     9181 ccatattgat tacatctaaa ggaatgcctt taggtgctag cttttcagca aattgatttt
     9241 gcataaagcg tacttgcggt cctaaaagta aaacgtctac atttttagat tctagttgat
     9301 tatccgcatc tgaagcagat acagcaaaga tgtctgcttc cattcctttt gcttctgctg
     9361 ccttttgcat ttttgtgact aggagacttg tactcattcc agctgaacat acaagcatga
     9421 ttgttttctt ttccatttta aacacgttcc tttctatact tacacttgta tataagcaag
     9481 tagcgtgcca actttaaaag cgctttaaaa agttgatatc atcacattta aggtctatac
     9541 caagttgcat acacactaaa aaagaaagcg cacactaaac acacaaatta atttttatta
     9601 ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca aaacttgtca caactttttt
     9661 caaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
     9721 taagatgttg tttttacttt gctattctta ttacttgatg caggacagct ttttcacaac
     9781 ctctcaataa acggtgttca atcagctgac cctgggtatt agctcaaaac gctggattca
     9841 caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa aacttctatt
     9901 tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt ctgataccac
     9961 gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg aactaatcca
    10021 tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca taacatatag
    10081 ccttcgcaat ttgcgccttc ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata
    10141 aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatatactg ttcttcatat
    10201 tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt ttcttttaga
    10261 tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga attattcatt
    10321 ttcttcccag gtaaatcaaa gacttcataa tatcgttcag gatggaaagg tgtctcttca
    10381 ttccattgat aacgaggggc gcgaacgcgt ttaggataat attgattgat tccaaggaaa
    10441 tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa ataaatcgat
    10501 tgtttttgac acaactccag gaactcttct gtgtagtatc cttttaccat cggatcaaaa
    10561 aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc tggagaattg
    10621 gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc ataatttccg
    10681 ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt ccacatcatc
    10741 cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca acgcgtttgg
    10801 ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta acgatcaaag
    10861 gcaatttgag catacttagc ataaaaatca actacttttc ttgaagacca gccatcatat
    10921 ttttccaaaa gaaaagctgg gacttcataa tgttccaaac aaatcatcgg ttctacgtct
    10981 gcttcgatca aagagtcgat catttcactt atatgctgag catattcttc atctactata
    11041 cagttctcat aatcagtaaa aaaacgagcc caattgatag aagtccgata atgtgtcaga
    11101 tggatggatt tcatcagatc cacatcctgc tggtattgtt ccataaaatt agttgctacg
    11161 gctgggccat acccattatg ccaaacaaaa tgttcatttt tataccagct atctataaac
    11221 gaatcctgtc cttctttttt tcctttccaa ccttccgttt gccaagcaga agatgcagca
    11281 ccaagaataa aattttttgg aatagtaatt ttcatgattt cctcctgttt tttagatccc
    11341 atcattttcc acacgatttg ctacttttac aaatggcaga tagatcaaaa tagaaacaat
    11401 aatacaaaca agttgcgtaa taactgcacc gatatttcct gctgttgcca gataagcatt
    11461 gacgatcggc ggcattggcc aagggaccat tacaactgct ttcgccgcaa atcctaaagc
    11521 ggttgcaaaa taaccaatcg ttccagtgat aagaggcgtt aagataaacg gaatagctaa
    11581 aattggattc agcatgatcg gcataccaaa aataactggt tcattgatgt tgaagatccc
    11641 aggagcaagg gataacttgg tgatttgttt catatcttcc cgttttccaa ctaacaatac
    11701 tgcaatcagt aaaccgatcg tgactccaga cccgcccata ctcatataca tatcccaaaa
    11761 tggcatagta ataatgttcg gtacttcttt tccagcttca aaagcttccg tatttacagc
    11821 aattgccgct aaaagcaacg gttcccgtat cggcttcacc atttgatttc catggatacc
    11881 aatcacccaa aagacttgcg caataaacat caatagcaaa atacccggta acccttgtac
    11941 gacaccttct aatgggcgtt gtacgacatt gtaaatgata tcataaaggt acattcctgt
    12001 aagttctttg atgataaatc ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa
    12061 taaagcagaa aaactagcag aaacattcgt tggcacggta tcaggcattc tcagctgtag
    12121 tttcttctgt ttcctcaacc agcaataaag ttcaatagaa aggatggaga ccaacattcc
    12181 caaaaataac cctttcgtat ctgtatattg tttagccaat acgttttcta ctataacagt
    12241 ctgattttca ttcactaatt cataaacagt tggattaaca gataaataag acatcactgc
    12301 gatgatacca gggaatacac catccacttt attcagcttg gctaattcga tccctatcag
    12361 aaaaacagca tagatggtca agaaacttag tgtgacatag ctgatggcag atgcgatagg
    12421 ctttaattct tctaaaaaac gcaagaaact tatctgtgct aagccatttt ccgagtcaaa
    12481 gaccattgct gaaaacaatg tgccaaaagc acccgtaata attaccggaa tcaatgcagc
    12541 aaatgcgttt tttatcacca tgatatacct taaattattg attctcgttg ccacgttccc
    12601 taaaacattg gctaatttat ccataaaact cattccagat cccctcattc ctgcttcttc
    12661 tccttgacgt catctctttt tgtctattag tcagcgctta catttcgatg aacagaaact
    12721 atttactcct cttttttacc aaatataaaa caaaaattca tctgatgtat ttatcataaa
    12781 atacatcaga tgaactgtca attagtttct ttttctccta ttattttctg taattctctc
    12841 cgatttattg ccatatggac agtcattgca tcaaacgcag caagtgcatt ccgcctttcg
    12901 atggcctgaa cgatctctgt atgaatatcg atcgtctcga ttttcagatg ctggatatca
    12961 taatttttat taaacaaagc aattgattca ttaatgattg ggataatatg cagcaaggct
    13021 acgtttccgc tagcttctgc aatcatcgta tgaaaatcga tgtctaattg aacatgttct
    13081 tgtgtgcctt ctcgaaaaga agtttcaatc ttttgcgtta attttttcat ttggacgatt
    13141 tgattcttcg ttgcatattc cgctgccaaa cctgccatct taggctccaa taaatatctc
    13201 agttcaaata agtctcgtat catccgatcc tgatcttcaa tcaaagaaaa accgagtggg
    13261 tcattggcaa tccctcgctt atcactgaca aatgtccccg ctccttgccg aacttcaagg
    13321 atattcctgg aagctagagc ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa
    13381 taatcggaca attcatattc attcggcagt ttatctccta cttgcaactg catttccttt
    13441 aaatatctaa ggatcttgtc agtagttact tctactaggg ttttattttc ttccaaacca
    13501 catacctcct tttcatctga tgtatttttt cgtaattatt ataacacgtt tgaatcatct
    13561 gatgattttt tgtatacaaa attgattaat ccgttcatag acatacaaac gaagaagata
    13621 tttaccataa aaaagactag gataaaattc actcctagcc ttcgatcatt tttattttga
    13681 acgacaaaaa atatgatgtt caataacaga cgatcttcat tcgattcttg atgtcatctt
    13741 ttgttttttt taatatatgg ataaaaagtt tgattacatc gtttctattc tagaatttgg
    13801 tcgattctca attcttcttc tggtgagaaa tctaaattat ccatcattcg aacactttct
    13861 tgtaactgac ttaaccgact tgctccgacc agtacacttg taacagattt ttgtcgcaaa
    13921 ttccatgcaa gggccatttg cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt
    13981 gctttgattt tttccagagt tgatccaact tgttcttctg aaaggaatgg tatttcctta
    14041 cgatgtgctc tagaatcttc aggaatacca tgtagataac gatttgtcag caacccttga
    14101 gcaagcggac tgaacgtgat tgcccctagc ccttcttctt ctaatactgg agatagatcg
    14161 tcttctagta gtgcacgaga aaacatattg tagcgcatct gatgaataat gaacggtgct
    14221 tcctttcttt ttaaaatttc agtcattttt tttgtatctt ctccattata gttggatata
    14281 ccaatatata atgcttttcc ttgtcttaca agttgcatca aagcttctgc tgtttcttcc
    14341 aaaggtgtat cagggtctgg gcgatgatga tagaagatgt caacataatc tagccccatt
    14401 ctttggagac tttggtcaca acttgaaatg atggatttac gagagcccca ttccccatat
    14461 ggcccttccc acatatgata acctgctttg ctagaaataa tcagttcatc acggtaggct
    14521 tgaaaatctt ctctaaaaat acgcccaaag tttttctctg cagctccagc agggcctcca
    14581 taattattcg caaggtcgaa atgggtaatt cccatgtcga atgcgcctct taaaatctct
    14641 ctttgattat ggataggatc ataatcacca aaattttgcc ataatccgag agataaaatc
    14701 ggtaatttca aacctgaatg accggattta cgatattcca ttttttcata acgtgcacta
    14761 tctgcaatat acaatttttc tcctcctcat caaatctttt taaaacgctt tcttttttct
    14821 atttttagtt tactacattt taaagaaaag gagttaatca aaatgatttt ttcgtatatt
    14881 tttttgccat ttgtttttta ctcgttgata gatcagaaat tgattacgtg cttgctggat
    14941 ttttttataa cgtttcggtt gttttttgta aaattgctga tgatagtttt ctgctggcca
    15001 aaacgtaccg gctggttcga tttttgtgac gattggttgg tggaaagttc ctgaactgtc
    15061 tagtttctgt tttgattgtt ccgctaattc tttttgtcgt tcgacttgat aaaaaatcac
    15121 tggacgatat tgcttccctc tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat
    15181 ttgccaatag attgtcagca actcgctata acttatttct cttgtgtcat aaatgatctc
    15241 cacagcttcc acatgacctg tgtaaccacc acttacttga tcataatttg gtttttcaac
    15301 atgtccgccc gtgtatcctg acaaaacaga taggatccct tttttcgatt caaacggctc
    15361 gaccatacac cagaaacatc cccctgcaaa tactgcacgt tcttgatagt cagcatcttg
    15421 catggcatgc ttggaaaagt catatttttg ctccttatca aatccttccg taatcaataa
    15481 ataaaagtcc attacatcag gcgtcagatt cattcggata gctaatggac gtagtgttgc
    15541 ttctagtttt gataactgtt ctttaacgga tatattttct cttatgtcat tctttgtctg
    15601 aatcaatact tttcgttccc aatctcgtgt attcggattc aaaatcagat tataaagtgt
    15661 ttgaatgaat atttgttcct catccataga ttctcctcct ttccgaagaa gacaagctag
    15721 tgactttagc tgtaatttat cataaagtga ggaaataagc gattacttca tccccctaca
    15781 gatttttcgt ataaaaaaga gagaatcctt atcgacagat tctccttttt aatcaattta
    15841 ttttataaat gaaaagttaa tttacctttt atggaaaact attttaaaaa ataaacataa
    15901 atatgcagtt aatgtaacaa gataagaaat aatagtcgtt actgtataac ttgtgcttac
    15961 tttaatatca gcaactcctt tgactccgct tatttgtacc aatccattag cattcacctg
    16021 ctcctttaca ggttgatgat ttatttctac attcgttcct aaataagcat atacaggaag
    16081 tgtgattact tggctcttct cattagttac tctatatatg atagaactac cagttgtttt
    16141 ataaggaagg tttacccatt gactgccaat ctttgcttcg tgttttatta tatgctcctt
    16201 attctccaat gaagcgatag gagaataatc atataaaaaa taatccattc ctttagtggt
    16261 atacgcctct actgtttctt tgtttacaac aatatgtccc ggctgagaaa ttattttttg
    16321 attgaaattt tttttactag aaacagttaa agtgattaat aacaaaacag tcataaataa
    16381 ataaaatagt tctcttgtct tattgtatct tctatttttg aaaaatatca tcgttaatat
    16441 cagagaagag aatattattt gcaagcttaa gatccgccaa ggaaattgta acaatttggc
    16501 tggcgtattt tgcagaagat accatggcac caaactagtt gtagctagcc aagtcaaaca
    16561 cgtaccataa taaatctttt tttctatgtt tgttaatttt ttaaataaaa tcaggggaat
    16621 acataaagat aacagaccaa ctagtcccat tgagtaagta gttaagtcgt tgttcatagc
    16681 ttcagtcaga ctatcgaaca cattagatgc atgagcatat aaatttgtca cgctaggttt
    16741 gttgattttt tggtagagca tttgttcaaa catcggatac caaaaagcac tcgtcagaac
    16801 caatgtcatt cctgccgcac taaataattt tttaaatata gcaataaaat ctgcattctt
    16861 gaaaccttta gaaaccaaac gaataaaaat aaaaatgata attaatgctg tactcattaa
    16921 tgttgatagt acatggctgt acactagcaa actcatactc aatgctagct ttacagacgg
    16981 atacttttct tcttttccag agatgatttg ataagcataa aataatacag ggataacaag
    17041 tgttattgcg atagcttcgg caattgcgga acgatagtag atgtctactg tccgataagc
    17101 agagaaaata tagagacaac taaataaaaa tgccgctttt ttttgtttta aaaaccgctc
    17161 gccataatga taagctacga gtgttgtaca caagttcaat aaccaaacgt aaataatata
    17221 gcttacaatc aaattttttg tcatccaata taacattaca gctggaaaaa aagtcagaaa
    17281 gggataaaaa taatttacac catatccagt ataattgaat gtctcataat taatcggact
    17341 ggtaaaaata gtcgataacc ccttgattcg agataagtga aaagaaagat ctccagtggt
    17401 ataaatactc tgtgtatttt tgaaaaagat gaaagaatat atactactaa ctataattaa
    17461 tccactcaat attactactt ttttatttat tttcattctt cttgtttatc tcctttttag
    17521 tcacatatcg agaatctttc aacttattag gtgcaaatcg tttaaaactt gaatacacca
    17581 ctaaacaata agtcagaata ctgatccata aagccatgag agcagttgga gtatattttg
    17641 ttgtaatttc tatcttattt tctccagcat ttacatccac attcactaag ccatgttttt
    17701 tataagagtc atttacgact ttaccgttta cttttacctg tgtacctaaa taagcatata
    17761 ctggtactgt cactttttgc tttttgttgc ttgccactgt gtaagtaatg ttattatctg
    17821 cagctttata cttccctttt tcccacgatt ctcctacttt tacttcatgt ttttctaagt
    17881 gaggcctttc ttttatcgct tctgttgggg cgtaatcgta taatcctccc atatctgatg
    17941 ttgtataacg tgggagtgtt tcttcattga taacaatatg accgtgtttt gattggataa
    18001 cttttgcgta attttctttt gctgctatag acgtgacgag tagaaacacc acagtagcgc
    18061 caatgattaa ccatgaatat tttttattca tagtcttttt cataaaaaca agcgttatga
    18121 tcattgaaga aaaaaagatt tggataccta agatacgcca aggaaactgg agtaagctta
    18181 tcggtgtttt ttgaaacaaa aaccatggaa ataaattagt tacgcatacc catgtagaaa
    18241 ctacgccaac atagatgatt ttttcttttt tctccatttc tttcagtatg aataaaggaa
    18301 aaatcaacgc aaccaatcct aataacccca tagtgtatgt tgtaatatcg ttatttaacg
    18361 cttgaatgat actatctgaa atagaaagtg cttctagctg caaatctgtt ttgaagggac
    18421 tgtgtatttc ttgataattc atctgctgca acataggata ccaaaaatag ctagttaatg
    18481 cacaagtcaa aaaaacagcc tttactaaat tcacgaccac aaattttata tcgtttttta
    18541 tttgcttgct taaaaacaaa cgaactataa tcatacaagc aatcaatgca ctacacaata
    18601 gtgtagttaa gacatgagta tacacaagta gacttatact gacagataaa gcgatgactt
    18661 ttttttctct attgaatatc aatccataag cataatataa aacagctggg ataatcgtaa
    18721 gagcgattgc ttctgcaatt gcggatcgat aatagatatc aactgttcga taagttgcaa
    18781 aagtatataa acaactaaat aaaaaagcag catctatctt tttaaaaaac ttttctccat
    18841 aataaaaact aatcagaatc gtacaaatat ttaata
//
LOCUS       DADVXN010000055        18049 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.057, whole genome shotgun sequence.
ACCESSION   DADVXN010000055 DADVXN010000000
VERSION     DADVXN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.057"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(57..1793)
                     /locus_tag="KB942_001629"
     CDS             complement(57..1793)
                     /locus_tag="KB942_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBB6852054.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1790..2494)
                     /locus_tag="KB942_001630"
     CDS             complement(1790..2494)
                     /locus_tag="KB942_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6852055.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2625..3128
                     /locus_tag="KB942_001631"
     CDS             2625..3128
                     /locus_tag="KB942_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBB6852056.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3088..3426
                     /locus_tag="KB942_001632"
     CDS             3088..3426
                     /locus_tag="KB942_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBB6852057.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3447..4865
                     /gene="ffh"
                     /locus_tag="KB942_001633"
     CDS             3447..4865
                     /gene="ffh"
                     /locus_tag="KB942_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBB6852058.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4977..5555
                     /locus_tag="KB942_001634"
     CDS             4977..5555
                     /locus_tag="KB942_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBB6852059.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5559..6080
                     /locus_tag="KB942_001635"
     CDS             5559..6080
                     /locus_tag="KB942_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6852060.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6159..6713)
                     /locus_tag="KB942_001636"
     CDS             complement(6159..6713)
                     /locus_tag="KB942_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6852061.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6966..7241
                     /gene="rpsP"
                     /locus_tag="KB942_001637"
     CDS             6966..7241
                     /gene="rpsP"
                     /locus_tag="KB942_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBB6852062.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7253..7504
                     /locus_tag="KB942_001638"
     CDS             7253..7504
                     /locus_tag="KB942_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBB6852063.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7629..8420
                     /locus_tag="KB942_001639"
     CDS             7629..8420
                     /locus_tag="KB942_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852064.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8590..9111
                     /gene="rimM"
                     /locus_tag="KB942_001640"
     CDS             8590..9111
                     /gene="rimM"
                     /locus_tag="KB942_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBB6852065.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9101..9862
                     /gene="trmD"
                     /locus_tag="KB942_001641"
     CDS             9101..9862
                     /gene="trmD"
                     /locus_tag="KB942_001641"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBB6852066.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            9998..10345
                     /gene="rplS"
                     /locus_tag="KB942_001642"
     CDS             9998..10345
                     /gene="rplS"
                     /locus_tag="KB942_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBB6852067.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            10718..11380
                     /locus_tag="KB942_001643"
     CDS             10718..11380
                     /locus_tag="KB942_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852068.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            11459..14686
                     /gene="fss3"
                     /locus_tag="KB942_001644"
     CDS             11459..14686
                     /gene="fss3"
                     /locus_tag="KB942_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6852069.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14800..15114
                     /locus_tag="KB942_001645"
     CDS             14800..15114
                     /locus_tag="KB942_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6852070.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            15127..15501
                     /locus_tag="KB942_001646"
     CDS             15127..15501
                     /locus_tag="KB942_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6852071.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            15511..15855
                     /locus_tag="KB942_001647"
     CDS             15511..15855
                     /locus_tag="KB942_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6852072.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            15926..17278
                     /locus_tag="KB942_001648"
     CDS             15926..17278
                     /locus_tag="KB942_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6852073.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            17338..>18049
                     /locus_tag="KB942_001649"
     CDS             17338..>18049
                     /locus_tag="KB942_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6852074.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGML"
BASE COUNT         6029 a         3231 c         3842 g         4947 t
ORIGIN      
        1 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
       61 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
      121 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
      181 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
      241 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
      301 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aattagattt
      361 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
      421 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
      481 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
      541 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
      601 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
      661 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
      721 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
      781 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
      841 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
      901 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
      961 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     1021 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     1081 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     1141 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     1201 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     1261 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     1321 ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     1381 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     1441 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     1501 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     1561 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     1621 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     1681 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     1741 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     1801 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     1861 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     1921 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     1981 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     2041 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     2101 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     2161 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     2221 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     2281 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     2341 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     2401 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     2461 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     2521 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     2581 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     2641 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     2701 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     2761 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     2821 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     2881 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     2941 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     3001 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     3061 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     3121 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     3181 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     3241 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     3301 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     3361 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     3421 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     3481 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     3541 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     3601 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     3661 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     3721 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     3781 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     3841 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     3901 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     3961 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     4021 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     4081 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     4141 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     4201 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     4261 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     4321 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     4381 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     4441 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     4501 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     4561 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     4621 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     4681 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     4741 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     4801 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     4861 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     4921 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     4981 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     5041 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     5101 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     5161 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     5221 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     5281 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     5341 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     5401 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     5461 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
     5521 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
     5581 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
     5641 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
     5701 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
     5761 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
     5821 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
     5881 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
     5941 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
     6001 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
     6061 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
     6121 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
     6181 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
     6241 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
     6301 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
     6361 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
     6421 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
     6481 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
     6541 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
     6601 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
     6661 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
     6721 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
     6781 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
     6841 taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
     6901 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
     6961 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
     7021 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
     7081 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
     7141 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
     7201 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
     7261 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
     7321 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
     7381 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
     7441 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
     7501 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt
     7561 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
     7621 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
     7681 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
     7741 ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct
     7801 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
     7861 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
     7921 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
     7981 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
     8041 aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag
     8101 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
     8161 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
     8221 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
     8281 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
     8341 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
     8401 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
     8461 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
     8521 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
     8581 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
     8641 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
     8701 gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
     8761 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
     8821 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
     8881 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
     8941 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
     9001 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
     9061 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
     9121 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
     9181 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
     9241 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
     9301 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
     9361 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
     9421 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
     9481 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
     9541 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
     9601 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
     9661 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
     9721 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
     9781 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
     9841 aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat
     9901 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa
     9961 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    10021 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    10081 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    10141 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    10201 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    10261 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    10321 tcgtatcaaa gaaatcagaa gataaacacg gaaaacttcc ttggaaatct gaaatttgtg
    10381 aaaagtcctt gtatatcaag ggctttttct tttttctcta tcacagtttt aagccatttt
    10441 tggctgtttt ggaccatttt tcaatcatat tcccttaaaa attgtcctag attgtccgac
    10501 attgactatc agtgtcgtca aaatagggca aaatttagta gtcagcttga cgacaaatat
    10561 aatacgaaaa ataccaaaac atttaccgaa tttagcaact tttgaaccca agtttctcaa
    10621 agggtcagct attagcaaaa aagacaaaac atgtactttt ctactaaaaa gttgatgtga
    10681 taggcttcta tctaaataac tattaggagt ttttatcgtg aagcaaaatg atggacgtat
    10741 catttggaaa aatcaaagtg agttaaaact gattttaaca attaatgaat tcatcgaaaa
    10801 acacggaatt acatctagcc gtcaatacca gaaaaagcta tcagaaaatc caaattctgc
    10861 accaagtatg tggttcatta acaaaaaata tggatcatgg gagaatttat tgattagtat
    10921 aggacgagaa aatactgggt atggaaaatg ggctagaatg tcagaacaag aattattgga
    10981 aatagtagaa gcctttatta agtgcgagaa gataacctct caacgaatgt acgaacaaaa
    11041 atcagtaggg aagaatattc cttcacttag taccattaaa aagatgttag gagatatacg
    11101 tccacttttt aaagaaaaaa atgatggatc tcgatttact gatttcgaac tgctgttaga
    11161 actaaaaaat gaaataatcc gattaaaact acaggatgat ttatcaatga caaagtttcg
    11221 aaaactagtt caatccccaa aactaccatc agttgataca attatgaaaa gaacgaacaa
    11281 aaattgggag gaattgatgg cagagattgg atttgattat cgaagaatca aaatttataa
    11341 gcagagaaac aacctatcca agacaaagaa aactaaatag tatcactcta aatcagacgt
    11401 tcaaaattaa aatttggacg tcttttttat tacccaaaaa cgaaaggaga tttattttat
    11461 gaaaaagcca agatttaaga attggcgttt gctcgcaaca ctgagtctat tatgccaaac
    11521 aattggtgga tcgcttggcc caacgattgc ctttgctgat gaaattactc atccacaaac
    11581 ggtaactgtg gaattggatt tggctcacca gtatgctgtc gaaggtacat ttagcgatgg
    11641 tcgtcccatg tcagaagtta ccgttccgca ctatgcagtt tacaatggtg tgaagcaaga
    11701 tattttctgt attgagccag gggtgccgat ttacaatgag ttcactcctg gttatgagaa
    11761 aaatccattg cctgatatgt cggagaaagc gaaattagtt tccgttttat ggaaggaagc
    11821 tggtacggat gtggatacac atattgtggc ccagaagatg atttggcaag aagttaatgg
    11881 ctacacgctt cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga
    11941 gtcaaaaatc aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac
    12001 agtgaaaacc gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc
    12061 ggagtttgat gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca
    12121 actagtgatt actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc
    12181 agctaataca ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg
    12241 ggcaattgat aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt
    12301 gaagattaaa aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt
    12361 agactttgga aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac
    12421 attggatgag attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc
    12481 ttatacgatt gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt
    12541 gacttcaaaa aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac
    12601 aggaactgat ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa
    12661 agacagtcca accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga
    12721 aacaccaaaa gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa
    12781 agctagtaat ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa
    12841 tcaaaccgtg gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga
    12901 aaccactttg acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt
    12961 taaaggagct gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga
    13021 ggcttttaaa ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac
    13081 tttagatgaa aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca
    13141 agaaaccaaa gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa
    13201 aaaggtggat gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca
    13261 agtcattcgc tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga
    13321 aactggcttc aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac
    13381 aggtgccgaa gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg
    13441 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg
    13501 atttcaaaaa attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta
    13561 cactaagagt gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac
    13621 ggtgaccgtt ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt
    13681 gatgctctat gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga
    13741 cggaggcaaa gaattgacag accttgattc taccgagcta gttgataaat tgagctataa
    13801 cttgcatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac
    13861 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc
    13921 aacgttggcg aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga
    13981 acaagttttg gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta
    14041 tgaagcaggt gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt
    14101 caattgtacg gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc
    14161 gcaaaccttt actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga
    14221 tgtactggat ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa
    14281 tacgaccaaa gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt
    14341 cactaaaacc gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt
    14401 taccttcaaa gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga
    14461 tctaaaagaa aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc
    14521 agaacaaccg gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa
    14581 gacattccca caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt
    14641 gatctttgcg actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa
    14701 acaactaaat aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat
    14761 ttttgtatcc aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg
    14821 ccagatatgg ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact
    14881 gaaggatatg ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag
    14941 caacgagccg atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat
    15001 caagatcaac cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag
    15061 aatcgtgggt ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa
    15121 aagaagatga gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta
    15181 cgattttccg cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac
    15241 gaggtaaaag aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt
    15301 agtatcccag caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc
    15361 aatccaatca tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg
    15421 ttcattaagg ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac
    15481 caaaatgaag gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat
    15541 tgtctcgatc aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc
    15601 tatgatgaaa aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgtc
    15661 gaagaagatt ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc
    15721 gaagtaaatg aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa
    15781 atgccggatg ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata
    15841 gcagtaaatg actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt
    15901 tcgattggag gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca
    15961 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
    16021 cgtcttttat tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat
    16081 agaaatcgtt cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg
    16141 aagcacgatg ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg
    16201 acaaaagcta gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag
    16261 tgaaggattt ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc
    16321 caaaatctat taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg
    16381 aaaatatcaa gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga
    16441 actagtagaa aaggaactca aagactccta tgtggagtac actttactgt atgatataat
    16501 tgccaatcga attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa
    16561 aaatcaagta tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg
    16621 tgggaagact tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct
    16681 ttttgttctt gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt
    16741 ctattcccaa aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac
    16801 tcgtagcaag gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct
    16861 tggacttcca ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac
    16921 gacgaaggaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca
    16981 atctggtttc tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg
    17041 gattcgcgat caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc
    17101 catgatgttt ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta
    17161 tgtagatacg ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata
    17221 tgatttctta agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa
    17281 taatagtatg tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg
    17341 acatttttag atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt
    17401 caccagtgta tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc
    17461 catgatacaa gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt
    17521 caatctctcg gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt
    17581 gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg
    17641 ttcgccgcta ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat
    17701 cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat
    17761 gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc
    17821 cttatcggac attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct
    17881 ccaacatctg atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg
    17941 gggggacaag tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc
    18001 aaaatcgcat tgccggtaga tgatggaatt tctgtagcgg gtatgttac
//
LOCUS       DADVXN010000056        17979 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.058, whole genome shotgun sequence.
ACCESSION   DADVXN010000056 DADVXN010000000
VERSION     DADVXN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.058"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            76..384
                     /locus_tag="KB942_001650"
                     /pseudo
     CDS             76..384
                     /locus_tag="KB942_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            385..702
                     /locus_tag="KB942_001651"
     CDS             385..702
                     /locus_tag="KB942_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6852075.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(859..1023)
                     /locus_tag="KB942_001652"
     CDS             complement(859..1023)
                     /locus_tag="KB942_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBB6852076.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1126..1647
                     /locus_tag="KB942_001653"
     CDS             1126..1647
                     /locus_tag="KB942_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6852077.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1634..2107
                     /locus_tag="KB942_001654"
     CDS             1634..2107
                     /locus_tag="KB942_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBB6852078.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2114..2272)
                     /locus_tag="KB942_001655"
     CDS             complement(2114..2272)
                     /locus_tag="KB942_001655"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBB6852079.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2365..3126
                     /locus_tag="KB942_001656"
     CDS             2365..3126
                     /locus_tag="KB942_001656"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6852080.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENYDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3346..4665
                     /locus_tag="KB942_001657"
     CDS             3346..4665
                     /locus_tag="KB942_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6852081.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4681..5901
                     /locus_tag="KB942_001658"
     CDS             4681..5901
                     /locus_tag="KB942_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6852082.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            5936..6613
                     /locus_tag="KB942_001659"
     CDS             5936..6613
                     /locus_tag="KB942_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBB6852083.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6610..7806
                     /locus_tag="KB942_001660"
     CDS             6610..7806
                     /locus_tag="KB942_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6852084.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7821..8144
                     /locus_tag="KB942_001661"
     CDS             7821..8144
                     /locus_tag="KB942_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6852085.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8148..8483
                     /locus_tag="KB942_001662"
     CDS             8148..8483
                     /locus_tag="KB942_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6852086.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8480..8875
                     /locus_tag="KB942_001663"
     CDS             8480..8875
                     /locus_tag="KB942_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852087.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            8872..9243
                     /locus_tag="KB942_001664"
     CDS             8872..9243
                     /locus_tag="KB942_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852088.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9259..9819
                     /locus_tag="KB942_001665"
     CDS             9259..9819
                     /locus_tag="KB942_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6852089.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            9946..10257
                     /locus_tag="KB942_001666"
     CDS             9946..10257
                     /locus_tag="KB942_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852090.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10281..10472
                     /locus_tag="KB942_001667"
     CDS             10281..10472
                     /locus_tag="KB942_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852091.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10472..14317
                     /locus_tag="KB942_001668"
     CDS             10472..14317
                     /locus_tag="KB942_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6852092.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14318..15190
                     /locus_tag="KB942_001669"
     CDS             14318..15190
                     /locus_tag="KB942_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBB6852093.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15187..16236
                     /locus_tag="KB942_001670"
     CDS             15187..16236
                     /locus_tag="KB942_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852094.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16240..17064
                     /locus_tag="KB942_001671"
     CDS             16240..17064
                     /locus_tag="KB942_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852095.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17082..>17979
                     /locus_tag="KB942_001672"
     CDS             17082..>17979
                     /locus_tag="KB942_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBB6852096.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQH"
BASE COUNT         6208 a         3092 c         4051 g         4628 t
ORIGIN      
        1 aagatatgtg caatagccaa acattataaa gttagcttag aatactttca ggatgattat
       61 ttggatagtg atgaacacga ggatattatt cctttgacag acgaaacaag agttaaatca
      121 gtgaaatcgc ttgaacaaag cctaaagtat ctgaataaca aacaagaacc taaccttcta
      181 gagatcaaac taaaagatac tgactcagta ccagaggttt attacaaagg tcagaagtta
      241 gagggattga tagatgtaag ctatcactac catacaacaa ctgatgtggt agctggttca
      301 acaaacgaca tcaatatgga gtacctaggt gaagtaacta agtatccaga tataaaaaca
      361 atcggacaca agagagatgt gtaaatgaaa gaagctagac ctagagacga gatagacaaa
      421 ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag ggacttcggc
      481 atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat ccatcacata
      541 gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga atgtatctgt
      601 gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa accaaaacct
      661 aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag cgaaatgaag
      721 gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt gctgtccttc
      781 cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc gaccaattta
      841 agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag aagtaggagg
      901 taatcggtcg cctggatcaa tttttacttc tcgtccgcct tggacatttc caccacgagg
      961 tcccacttct ttataggttc cttttggttt attgtcttcg ccgggtttat agagttctcc
     1021 cataggaatc cctccctaaa aaaatttcgg cacagcactg ccgataactt aattataagg
     1081 attgtgataa cgatttcaat atatcttttg aaagaaggtg atattatgcc gcaaccagcg
     1141 aagagtgcaa aattacaatt attaaacgga aacccaaata agaagaatac cgaagaactc
     1201 cgcaagcgag cggccgcaga agacaaatta aaaatggcta ctgacaaaat caaaccgccg
     1261 ttatggctag attcgctagg aaaggatacc tttgagttta tcgctgatga attgctgtct
     1321 gtggatttaa tcagtaatcc ggacgtccat acaatggctc tctactccaa ttggtattcg
     1381 caatacgttt ctttagaaaa acagcttcga aaactacaac gagagtacaa gttgaactat
     1441 gcgcttgcga aaaaggaggc agaggcgaga ggtgagccgt ttaatgaacc taatgaatta
     1501 attggtaatc cgctctctcg gcagatggat acagcgtcgc ggaatctccg ttcttttggc
     1561 gctgatttag gactatcacc agcagccaga gctaagttag ctattaagat ggctgatgat
     1621 ggtggtgatg acgatgacga cttctaatat tttggatatg tcctacacag aacgtgtgga
     1681 ctattggcaa agctatcttg aggagcaagc ttcttggggt ggctttttaa aatgtccata
     1741 tccggaattg ttaactactt ggtatgcgga acgattaatc gatggaagca taccagccag
     1801 caaagaaaat attcaagctg ctaaacggca tatgcgtgat ttgcagcgcc aaggaacaga
     1861 tgattttcct tggatctttg acgaagaaaa aggtcaccgg cctattagat atatcgaaaa
     1921 aaaatgtaaa ccaactgaag gcgactttgg ttcgtttgtt ttgcaacctt ggcagcattt
     1981 cataattgga tccatgtacg gatgggtaca tcgtgataca ggagagcgtc gcttccgcga
     2041 ggctcttatt tttgttggac gtaaaaacgg taagttttgc cgtttaaaaa tcgggcaaaa
     2101 tcggtgaaaa cctttatttt tggctttctt ttagtaataa ttcgatagct ttatcaagca
     2161 atttagacat aggaaccata gtttcttccg acattttttt taatccttcg tataaatctt
     2221 tatcaattgc gttagaaatt ctaattctgt tttttagtcc gtatttgtcc atgtgtgaca
     2281 ctccttttta ttttactata acatccgatt ccaccgcttg caactaccat caattgatgg
     2341 tagtatataa aagaggtgag tagattgaaa aaatacatta tttacaaaca cttaaataaa
     2401 ataaatggca aaatttatat cggtgtcaca aatgatattg gcagaaggtg gaggagcggt
     2461 gggattgaat ataaaccacc aaagaacgaa acccaacacc acagaagttt ttggaatgca
     2521 atacaaaaat acgggtggga aaattatgat catttgatta ttgaagaaga cttaacgatg
     2581 aaagaagctt ttgagaaaga aaagttttat attgaacttt atgactcaac taataagaaa
     2641 aaaggttata acatcgcaaa aggcggaaat ggcggaataa tatataaagt tcaccccaaa
     2701 ggaatgctag ggaaaaaaca atctaaagaa ttttcttcaa atcacagtaa atgggcaaag
     2761 aaccataaga ataattgtat gacaaacggt gatgttgtat ggggtgttac tcacgaacac
     2821 ccaaaaggca tgttgggaaa gcaccacagc aaagaatcaa ttatgaaaaa gaaagcatat
     2881 agtggtgaaa atgctgtaac ctcaaaacca attgtcgcaa ttgaacggga tggaactaaa
     2941 agagaatttc acagcgctaa gctttgcatg gcttactatt cgattagtac atgtgttttt
     3001 taccgacttt taaaagatgg cgccccttat gtgataaatc ctaaagcgaa ctacagaaat
     3061 aaagaaaaaa tattagctat cgaaggaatt atgttcaaac ataaagaaga taccgaggta
     3121 agttagtaga ttgcgaaagg ttactaacta ccgtaacgcg taggagttga ataaatataa
     3181 tgctcccaag agtgtccgac agcagataat ttgctgaaaa tgtacgctaa actgggccag
     3241 aactgactga ccgatgaaaa tgagggtgac ctccagagtg cgagataaaa agctcgcaga
     3301 taataacaat cgaaaacatc actaatttca ggcctttcca catacatggt cgcttatgat
     3361 gatgaacaag gcgccaacgt ttacgtattg gcaaatgctc gtgatcaagc aagcttgttg
     3421 tttgataagg ccgcagaaat ggtcaaacaa tcgccggcgc tctttaagaa atttggtaag
     3481 cctaaacgat caagtattaa ttatgctccc gccttttcta aaatggaacc acgcgcctca
     3541 gatagccgga aattggatgg gctaaacact cactttggta tttttgacga gatccacgag
     3601 tttacgaatt acaatctgat caacgttatc aagaaatcaa gaggaaccag aaaacagcct
     3661 ctgatagttt atatcacaac tgctggatat gtattagatg gtccgttgat gtcttatttt
     3721 gagcaaggtg tggactgttt ggaacatttg gaagatgaca tcgatgaacg gactttctat
     3781 tatctggcaa aacttgacag tgcggaagag gctgatgacc caagattatg gatcaaagcc
     3841 aatccgaata tttgtctaat gaattttgtt ggcatgctag atgactatgt taaggataaa
     3901 aaagatccaa aagaatatgc tgactggatt accaagcaat ttaacttgtt ttccgatatc
     3961 gatgagctgt catttgtcga tatgcctacc attaaacgaa acaataaaac catcgatatt
     4021 gaaacgctca aaggtaagaa gtgtgtcggt ggttttgact tgtccgaaac ggaagacttt
     4081 accgcagccg ttttagaatt tccgcttgaa acaggcgagg tattcatttt gcaacacaca
     4141 tggatcccac aagctagatt tgatcgagat aacaatcaag agcgtatcaa agcgtgggag
     4201 aaggtgggag atctaacgat tattcctggt gattacgtca attatgaata cgtcttaaat
     4261 tggtttgtag aaaattcgaa aatctatgac attgtaaaaa tcaattatga caaggccaag
     4321 gcgctacgat taaataaaga actagaaaat gcaggatttg aaaccaacga gattcggcaa
     4381 gggtttctat cattaggtgg gccaatgcaa aacttcaagg aaatgctatt ggacggtaag
     4441 gtgattttca acaattccaa gctttaccga tggtatctat ccaacgtcaa gctggtgatg
     4501 gatcgcaact caaactggat gccgtctaag cagtccaaga gtagaaaaat agatggtttt
     4561 gcagcaagtt tgaacagcca cgccgaagtg ttgaatatgt tggttaatcc tgtcggaacc
     4621 gggaaagtaa cctattactc gatttccgat ttaatgaata tgtaagaaag gtgtggagga
     4681 atgagtattt tagatcgttt gcgttctttt ggccgagcga agccgaaagc gagcaaacaa
     4741 gagtattttt tgaatgaccc gggattgata ccgtatttag tcggaaaaga tgaaatatca
     4801 gaagggattt tttccgtaat tagccgtgta tcgaacgttt ttgcgtctct ccctctcaaa
     4861 atgatagatg cggagtttgg ccaaccggac gactgtcctg catacaactt gttgagcgaa
     4921 ggccctcgat attttacaaa gtttgatttt ttccgggacg tggaagtttt gagaaactac
     4981 caagggaatg cgtatgtgca gattttccga aatatcaatg gagaagtagc agatatggcg
     5041 ttaataaaac ctggtgcttg ccatccagtg attgatatgg atagcgggga gctttactac
     5101 caagtaactg cgactgacaa aggcagttat aagcaagtta tttatgtaca ttacatggaa
     5161 atgctccact ttaaacaacc gaggtttggt ggcttggaag gtacagaccc cacaaaagta
     5221 ttaacgaata ccctcggata tgatcgagaa gtccgaaaaa tctctttaag tcagcttaaa
     5281 ggaggtaatg aagggctaaa agttaagttt gctagcaatg tggatgaaga agctaaaaaa
     5341 gctacagtta aaaacattgc tgatttttat cgacaaaacg gtggactact tgtggaagaa
     5401 aacggtgtag aaatcgaacg tttacaacga gagctggtag acagcaagct tttagatact
     5461 gataaagtat ctcgctccag aatcgcgatg gtctacaacg tgccggaaca tttcattggg
     5521 agtaaccagt cgagttactc ttcacaggaa cagctcaata tggagttttt gacatacaat
     5581 ctagtaccga ccgttaatca atatgaagcg gaactaaata agaaaacact atcgagaacc
     5641 gaaaaagcaa aaggttatcg atataagttt aatgtcgcaa gtttgctaag agctgacact
     5701 caggcaaggg ggcaattcta tcaaatcatg cgacgaggcg gagcatactc agcgaatgat
     5761 gtccgaagat ttgaggactt gcaaccgata aataaaaccg gtatggatga ttatcatatt
     5821 tccggagacc tatatccaat cgatatggat ccaacattaa gaaaaacaaa ctcgtctaaa
     5881 agcgtagccg aaaacggtta ggctttttta gtttgcaccg aagggaggtg aaaggatgaa
     5941 aaaagtgacg ttaagcggcg atgtcgtgga taacgatacc gcgtggttat atgactggtt
     6001 tgggattgat tgtatctcac cagggaaaat ttctgccgct cttacagaag cagcggggga
     6061 tgaagtagaa cttgatatct catctaacgg tggggatgtc ctagcggcaa gcgaaatata
     6121 taccgctatc cgtgcctatc cgggaaaggt atccggaaat gttgtgagta ttgcagcaag
     6181 cgctgcaagt gtaatcgctt gtgcttgcga accgcttaga atctcgccaa cagcacacat
     6241 catgattcac aacgcatggg tgaccactag cggcaacgcc gaggaattaa aagccaatgc
     6301 ggaaatgtta agcagtgtgg atgagtctat cgttaatgct tacgagatca agacaggact
     6361 cgaccggaaa aagcttgctg atttaatggc aaaagatact tggttaaacg cacaaacagc
     6421 agtggcggaa ggttttgcgg atgaaattat gtttgcagaa gcaccagtaa cggtactcaa
     6481 tgcctctcaa ccggttattc caaaaaacgc ggtaactaag ttgaaaaatc taatactcaa
     6541 agcggaaaca ccgcaagaag aaacgctctt gcagaaaaaa ctaaaagcct taaatggagg
     6601 gaaaaacgaa tgaatttaga acaattaaaa aatgcgtggg tcgaggcggg aagtaaagtc
     6661 tctgatttaa acgcgcaact caatgcagca ttggttgacg atgaaaaaac agaagaagat
     6721 gtagtaagtt tgcaagcaca agtaaaagca gcacgggcta aacgggacgg attgaaagag
     6781 caagtggcaa atatggaagc cgaacaagtc ttaaacgtca aaaaagaacc attagataaa
     6841 aaagatgaaa acttgaaaaa caagtttatc aaagacttta aagcgatggt caatggtgat
     6901 cctgctatta tggctacttt gacatctaat acggatgaat ctggtaatgc tatcggattg
     6961 actattcctg tagatgtgca aacgactatt catactttgg ttcgtcagtt tgactctttg
     7021 caagaatacg taaacgttga aaaagtgacc actactagcg gttctcgggt ttatgaaaaa
     7081 tggtctgata ttaacccact gaccgctttg gatactgaag acggtgaaat cccagcaaat
     7141 gatgatcctg cactttactt gatcaaatac ttgatcaaac gctacgcagg tatttctaca
     7201 gtaactaaca gcttgctaaa agatactgcc gaaaacattt tggcatggtt gtctaactgg
     7261 atcgcgaaaa aagtagttgt tactcgcaat acaaaaatct tggcagctat tgatggaatc
     7321 aaagcagcgc aaaagaaaga tgttacagat gttgatggga ttaaagatat cataaacgtc
     7381 caacttgatc cagctatcga agctacatct atgtttatta caaaccaaga tggcttcaat
     7441 gttttagata aagtgaaacg tgctgatgga tcttacttgt tacaaaaaga cgtagcttct
     7501 gcaactggat ataccttctt aggtaaacca atcaagaaaa ttgcttctcg tttcttgcca
     7561 aacaaaggta cgcaagctac tcctaaatat ccactgtata ttggtgatct gaaagaagct
     7621 gttacattgt atgatcgcga aaacatgagc ttgctgacaa cgaatattgg cgggggggct
     7681 tttgaaacag acaccaccaa agtacgggtt atcgatcgat ttgatgtgca gttagtcgat
     7741 gatgaagcag ttgttttagc tacatttaca tctatcgcaa acgaagtgcc agtaacaaac
     7801 ggttaaggag ctgatttctt atgattcttg atcctaaaat ggatttagac gaaatcaaaa
     7861 acgcactaaa gattgatacc gatgatgacg atgtggaagt aagacgtgcg gtgcaagctg
     7921 caattgcata cattagaggg gctatcggga atgataagcc ctctttttat aagcaagaaa
     7981 atgacacggt tgatctgatt aatttagcta ttctgcaatt agcagatcat tattacaacg
     8041 ctcgatcagc aaccgtgagt gggaacttgc gagagtacga tttaggtttt acaagcctaa
     8101 tcttgcaact caaagcaaga tatttgcttt ttgtggagga gtagcgtatg ccccttattc
     8161 aaacaggaaa tttaaatcaa cgcatcaagt ttgtccgaga tacgacggtc aaggacgagg
     8221 acgggcaagt tgtccggact tctacaacca ttcttacttg ctgggcaagt gtgcagacac
     8281 aacgcctgaa cgatattaag acgtccattg gtacggcttt ggaaggaaca ctgacgttca
     8341 ttatccgcta tcaacaaaaa tcagagctaa ccaatgatat gaaagtgcgt tggaatggaa
     8401 aaacgtttga aatcattacg attacgaaag gcgagtttgc gaaggacttc acgacaatca
     8461 ttgcaaaaga ggtttcaaaa tgagtgtaga agtcgatgca accgaagtgt acaaagcgct
     8521 tagggaagta aaagcaaacg ttcaacgagt ggaaagccca gcacttagaa aagctgggga
     8581 gtacgctcaa gaaaagttac aacaaaacac accttactgg gatggaacga agtcaaacgg
     8641 taaacgtggt tcgtatatgc aagaacatgc taaggaccat gtggttacaa gctcggtaaa
     8701 aaacggattg atagaagtcg gctatgataa agatgtttct tggcggatgc actttatcga
     8761 gttcggaaca atcaaacaac gtccaaaagg tttcgtacaa aaaacacaaa agcaaatcga
     8821 aaaacaagta acacaaatca ttgctgacga agtaaaaagg aggctaggac tttgaaaacg
     8881 gcagtatcac aagtctattc aattctgaat agcaatgaaa aaacaaagaa cattgatttt
     8941 tacaccaata gtgttccgga atcggctcaa acagtaccta gccttccagt tggcagaatt
     9001 acagagatat tcggcaacta tgaagatttc gcaagcaaca atcctttgac cattcaattt
     9061 aacgtacagg tagatgtatg ggtatcgacc ttaaaagagg ttgatgcctt ttattttgcc
     9121 cttgatgaga ttatgagggg gaatggttgg caatgcgcat acacggaaca aacagatgat
     9181 gaggatttag aaggtgcaaa gcggattatc aaacgatatg tagcaaatat ttcactaaat
     9241 taaaaggaga gaaaatagat ggcaacagta ggatttgaga gcgtcatttt tggcgtaaaa
     9301 acaggtgcag gcggcactct aaaagaatta gtagcagata agtcgaaagg cggagcgatc
     9361 gaagctaaaa ttactggatt aggcgcaact tctaacacaa catacgcttc aaacgtacca
     9421 ttcttcattg caagtaaagg ggtttcgtcg ccaaaagtta cgcttgacgt ggcagactta
     9481 atggataacg gcatttacag cgaaatcatt ggcgctaaaa ccgtggatgg tgtaaatgta
     9541 attggttcag aaactgaagc gccttacgtg tcggtagtca tggttacagc gaacaaagaa
     9601 ggaaaacgct tattcatggg attggcaaaa ggaaaattca gtcatccaga tatcgacatg
     9661 aaaacagctg aagacaaagg agttgaattg caaaccgatt ctatcgaagg ggaattcatt
     9721 tctgatgaac gtggctatgt atacttaaca gccgtagaat cagaagaaat gaccttacaa
     9781 aaattcaagg acttggtaaa taacaaagcg ggggagtagt taaccctgca tctacaccaa
     9841 cgacagatac agggacacca aaagaaccag aaccaaaaat tgatacacaa ggttagccat
     9901 tttttggcta gccttatttt tttgtaaaaa caaggaggaa aacaaatgat tgaattgcaa
     9961 ttgaaacttg acggaaagaa aaaaacattc aaacaacaag atatttccgc acgtgcaatg
    10021 cgtgagtgta tcaaatttta cgagaaagcg gaaaaagcag acctaactga tttagaagca
    10081 attgattcaa tgattgcaat tacagcagat attttccaag atccagcagt tacatttgat
    10141 gctattttag acggtttgac tgcgagcgag ttagtaccgg cattagaaag tgtttttgaa
    10201 caaatcaatg aactgggaaa caatgaaaaa aagcagacgg cgagcaaaaa gagataagtt
    10261 tttctgaagc taggaaagca atggatcaaa tctacaaaga tttaatcgaa tcaggttgga
    10321 cgatgagaga tgtggacgaa gccgactatc attatttgtt acaccttttt ggagaagtgg
    10381 agagtggcga agaatatgta gatggtgctg atttcatcaa acaattttta tcggctgaag
    10441 acttggtaaa acttgaggaa ggaggtaaat aatggcagga aaaggacaac cggcaggaaa
    10501 tatcaagcta gggattagtt tagatagcac taattttggt aacacgctgg acgaaatcaa
    10561 tgcgaaagtc aaacaagctg agtcgaatat gcgtgccaat ctaaaggctt atgattcagc
    10621 aggacgttca tacgaagcac ttagtcaaaa gacgaaagac ttgtctacgg ttatggaagg
    10681 gcaaaacgcc aaagtaagag aattaacaaa gcgccgtgat gaagcgatta gcaagtatgg
    10741 cgaggaatcg aaacaagttg ctaaccttaa cacacagata aacaatgcta ccgcaaaata
    10801 taatgcttac agtcgccagt tgaatgacac aaaaaaagaa ttggtgtatt ccaaaacagc
    10861 cgtcaatgat ttatctaacg aaatcaaaga aaatgaacga caaatgaacg ccgaagtaaa
    10921 agcgttgaaa gccgctggtg atgaatctgg tgcgtttgaa gcaaaacaaa aagggctagc
    10981 caaacaaacg gaattatccg agaaagctat cgaagaacag cgcaaagttg tgaaactgat
    11041 ggctgatgag tttggcgatt cagcaaatga aaccgaagat gcaaaaaggg cattagaaaa
    11101 gttagaacga caaagccaaa tatctagcag gcaattagaa gcactcaaaa gctccagcga
    11161 tcaatcagga aaaaaaatag aagattttgg cgacaagtcc acaaggtcag ctaggaaact
    11221 ggatgggcta aaagacaaat taggctcgct aaaaagcgca ttttcgtttg gtgcagttgc
    11281 tggattagcg cataacgcta ttagcagtgt agtaagtggc gtgcaaggct tggttggcga
    11341 agcagtaaac gcatcggatt cattgatgaa gttttccaaa accatggagt ttgctaactt
    11401 tgggaagtca cagatagaaa gctcgaaaaa agaaatgaaa gactacgccg ataagacggt
    11461 ttatggttta gaagaaattc tgaacacaac cgcacaattg gcatctaacg ggattcctaa
    11521 ctatacagaa ctaaccaagg cggcaggtaa cttgaatgcc gttgcaggcg gttctagtga
    11581 tacattcaaa tccgttgcca tgatgctaac gcagacggca ggagctggga aactaacaac
    11641 tgaaaactgg aatcaattag cagatgcgat accgggcgct tcagggctgt tacaagacgc
    11701 tatgttgaaa aacggagctt atacaggaaa cttccgtgat gcaatggcgc aaggtcaaat
    11761 cacttctgat gagtttaacc aagcgattgt acagttaggt atgaatgacg gagcagttaa
    11821 agcagctact tccacagata cattgagtgg ttcttgggaa cagatgaaat ctactgtaat
    11881 aaatgggtta caaagtatca tagacaaagt aggcgttgaa aatatcactg gtttcatcaa
    11941 cagagtaaca aaagggattg aaaattctat tcctaaaatt actcaattta taggttggtt
    12001 gagagatatt ggaacgtgga tcgttgaaaa tagagagcca ctaacatgga ttgtcggaat
    12061 cataggcgga attacattag cagtaaaagc attgaacgta gcaagtatgt tgctggcaat
    12121 tactggcgga acattggcag ccccttttgt ggcgattggt gtagcattag gcgcactagc
    12181 aggtgctttg gtgatagctt atacaaaatc tgaaacattt agaaatatag tcaatgcggc
    12241 ttttacagct gtgaaaaacg tagttatgag cgttatcaat aatttggtgg aatactacaa
    12301 aatgttgtgg agcgtgttgc agtggctttg ggaaaaaata aaagaatggg cttcatggat
    12361 tggcaataaa ttcattgaaa tgaagaacag cgttgtgaat acggttcaga acctatggaa
    12421 tggtgtgaaa aacttcttca gtaatggcgt tggagacact tggaataagg tagttggctg
    12481 ggtaaaaaac attttcaaca aagcaactga attgaagaac aaagtttctg atgcaattgg
    12541 taacctgtgg aacggtatca aagacacatt ccgtagaggc attgatacgg tattcaattg
    12601 gttttcagaa ctaccgacga agatgaagaa tgccattatt ggcggtaaaa atgccattgt
    12661 tgatgcgttc aaaagtattt tcaacgcagc acttaaagcg ataggtaaac cagttaacgc
    12721 aatcatccat ggagcttcat gggtactaga aaaactgggt gctgacaaac tcgaagaatg
    12781 gaaagtgcca caatacgcaa aaggaacacc aaacggaggt catccgggcg ggcctatgat
    12841 ggtaaatgac ggtagaggtg ctgaagcggt aatcacacct aacggacaag catttatccc
    12901 acgagggcga aatgtagtgt tgaatgcacc aaaaggcaca cacgttctaa cagctgaaga
    12961 aacagcttat atgactggaa acaaagcacc aagatataga tacgccaaag gtacaggctt
    13021 tttcggaaat ctatggaaca acgtcaaagg atttgctgga gatgttggaa acaagctgaa
    13081 agatgtagtc ggcgatgtat gggattttgt aacagacccg ggagcgttgg ctaggaaagt
    13141 gttaaatggt cttggcgtac tggaagggct tgtcaaatat cctttagatg ttggtaaagg
    13201 tattctaagc aaggctaccg aagcattgac gaacaaaatc acagaactat tcagcagtgg
    13261 cagtttagac acttcaatgg gcatgcaagg cgtttacaaa tacttggcgg acgttgcagt
    13321 tgcagtaatg aagaagtttc caggctttgt ggcaactagt gggtatagac caggtgaccc
    13381 ctattcgcat ggtaaacgta atgccattga tattgcacta cctggtgtca caggaggctc
    13441 gccacgctac acagaagcag ccaattacgc atttgagaag tttgcaaaca aaatcggcta
    13501 tgttatcaca aatggcaagg ttcgtgaccg ttcaggacaa tcaggtacag gtattcacaa
    13561 tgattggaga ccatggcccg atggagatca ctatgatcat gtgcatttaa acggtgtgaa
    13621 agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg tagaaagatg
    13681 gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg caaacttaaa
    13741 tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag ttaacaattg
    13801 ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga ttgacccaac
    13861 attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac tatctaacat
    13921 cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg cctatcgtgg
    13981 agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa tcgcagaagg
    14041 aaacaagcca gaaattatta ttcctttaga taaggctaaa cgatcaagag cgatgcaatt
    14101 attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa atgcgacaac
    14161 tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa caacgaatca
    14221 gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata gagatttagc
    14281 tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat tactgagaaa
    14341 tagaataaac gaagaaattg atttgtctac ggaggattat tttgctactg ggttgagtaa
    14401 tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca gagaaagcag
    14461 tgagaacgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc atgggtttcg
    14521 agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt ttgaactgga
    14581 atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt taagtaagac
    14641 tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt tcacatcaaa
    14701 ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga aaacacgtgg
    14761 taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg agaaaaaagg
    14821 tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac ggatgtcccc
    14881 gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa tccaaaactc
    14941 acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga ctctagaagt
    15001 atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg tagaatcttc
    15061 tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccga ctggagaatt
    15121 cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg aggagcgtga
    15181 tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta taacgaacat
    15241 ttattctcta acagttttga atttggtgcg gacgagatca acgaagaggc tagtagcttt
    15301 acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa atatattcct
    15361 agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa aatcagttgt
    15421 aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc aggagataac
    15481 tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc aacgaaacaa
    15541 ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta tcaagctacc
    15601 gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa atacaacgta
    15661 aaatggtatt tcaagggaat tcaaaacaga aaaatctata caggcatacg tgctgtaaat
    15721 gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt gtttgttcag
    15781 gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat gaaagatata
    15841 gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt gacacaagat
    15901 ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta cgatcaaacc
    15961 gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg caatacgtat
    16021 tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt cgaaacgatt
    16081 gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc ggttttgaca
    16141 gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa cataagaagt
    16201 cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa tgtggatgga
    16261 tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta tcacgtttta
    16321 tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt atcttcaagc
    16381 ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat ggtcgttgtt
    16441 cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc attaacagtg
    16501 gatttgacac aagaagttat acctggatct attcttccag aatcggaaga atatgaatgg
    16561 actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa tttaaacaat
    16621 ggtgataagg tctataattt accactatgc tcagttactt ctactggatc aactgtttca
    16681 atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat tttgtggagt
    16741 gggactgcgt taatgcatga tactcaaact gtccaacctt caaaaaaaat gtggcagaca
    16801 gtttcaggtt ttttattagt atggctccca tacgaaaacg ggcaagcaat tggggataga
    16861 tatgtaacta cgccttttta taaagagcgt gtaactttta ctaatgtttt aggagaaatt
    16921 gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa ctataattcg
    16981 aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc aaatatggtg
    17041 cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat ttagaaatta
    17101 aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt agagttggtg
    17161 atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt acaccttttg
    17221 taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt aactataccg
    17281 agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg ttcactaagt
    17341 caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta gatcctaacg
    17401 gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagta gatattaatt
    17461 cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa atgcaaacca
    17521 ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac tcgaaatata
    17581 acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt attgataaga
    17641 caaatgatcg tattgatcaa accaatcagc aaatcggcga tctcggcaag ctgaaaaaga
    17701 tgtacagtaa cagcatcgac ttcggggact atgattatag tgggaatccg aaccttagcg
    17761 ctaaacttaa tgcttcaagt ttttctttag gtactggcgc aacagtcgcg gatgataatg
    17821 atgagattgt gtttacctta gatggcacaa atcaactatc gaagtacgct agcaaagcgc
    17881 aagtgccggt agcagaaggg aaacaatata cgattagttg tgaaattatg ttagaagatg
    17941 ggttcactgg cgacccttca ggaatacgtc tgcaacacg
//
LOCUS       DADVXN010000057        17757 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.059, whole genome shotgun sequence.
ACCESSION   DADVXN010000057 DADVXN010000000
VERSION     DADVXN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.059"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..135
                     /locus_tag="KB942_001673"
     CDS             <1..135
                     /locus_tag="KB942_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6852097.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="KB942_001674"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="KB942_001674"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBB6852098.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="KB942_001675"
     CDS             complement(2412..2663)
                     /locus_tag="KB942_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBB6852099.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="KB942_001676"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="KB942_001676"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBB6852100.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="KB942_001677"
     CDS             3613..3831
                     /locus_tag="KB942_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852101.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="KB942_001678"
     CDS             complement(4120..4632)
                     /locus_tag="KB942_001678"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBB6852102.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="KB942_001679"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="KB942_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBB6852103.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="KB942_001680"
     CDS             complement(7085..7528)
                     /locus_tag="KB942_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBB6852104.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="KB942_001681"
     CDS             complement(7529..8317)
                     /locus_tag="KB942_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6852105.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="KB942_001682"
     CDS             complement(8314..9255)
                     /locus_tag="KB942_001682"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBB6852106.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="KB942_001683"
     CDS             complement(9432..10019)
                     /locus_tag="KB942_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBB6852107.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="KB942_001684"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="KB942_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBB6852108.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="KB942_001685"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="KB942_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6852109.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="KB942_001686"
     CDS             complement(12936..13871)
                     /locus_tag="KB942_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6852110.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..15016
                     /locus_tag="KB942_001687"
     CDS             14057..15016
                     /locus_tag="KB942_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBB6852111.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15102..16196)
                     /locus_tag="KB942_001688"
     CDS             complement(15102..16196)
                     /locus_tag="KB942_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6852112.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16385..17146
                     /locus_tag="KB942_001689"
     CDS             16385..17146
                     /locus_tag="KB942_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6852113.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17261..17701)
                     /gene="fabZ"
                     /locus_tag="KB942_001690"
     CDS             complement(17261..17701)
                     /gene="fabZ"
                     /locus_tag="KB942_001690"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6852114.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5114 a         3864 c         3106 g         5673 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14401 aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg gccatcagga
    14461 aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata attatcctga
    14521 tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc atcataaaag
    14581 tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta aacccaacgg
    14641 ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct tccctaaaaa
    14701 cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga aagccattag
    14761 taaccaacat gatattgata tatattttgc ggaccctgga acaccttctc aacgcccatt
    14821 aaacgagaat tctaacggga ttctgcgtcg taatggactg ccgaaatcaa tggattttag
    14881 aaaagtgaat cagacattta tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa
    14941 atcattgaat tacagaacac caatcgaaat atttttgagc tatgtacaag aagcatttta
    15001 ttctagctta atttgacaaa tcataaccta taaaaaaaca aaataaaaaa gcagaaataa
    15061 ctggctattt ctagcggatt atttctgcaa taactaatta tctatttttt attttcaatt
    15121 aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga ataattcgtc atcggagatt
    15181 cttcctacaa cattgacacg tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct
    15241 cctttgtatt gaccgtaccc ttcattgtca atcagtacat ccccatgatg catatctcgc
    15301 gtattatgag gcgggaaatc tttatctttg tagtatacac gagtcatcgt tgaacgaagg
    15361 atatattctg agcgatcccc tcgataacta tgaaggttat caaacaagca gattcgttcg
    15421 ttttctgtaa taccttcttc cgtatctact ttcaatgttg gataatccgc gttaaatgct
    15481 tctgccattt ctttcaattc ttcttcactt gcgtacgcat ttccaataga aatatcatca
    15541 atcaatcctg taaggattaa atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct
    15601 tccagcgtgc acaagccatc ttgcgtaggc caagggccaa actcagcaga ttgcgaattg
    15661 acgaatgcca tcgtattcaa attatatttt ctgaattttt ctgaacaaaa aacaaagtgt
    15721 tcgtatccta aaccagagta tcgatgagga taaaaattat gagaacctaa taaattatcc
    15781 gtgtttggcg aataactcat gatgttgtca acgtaattcg tgcccgaact catattgatc
    15841 tcgattttga taccaaaagg attgcgcgtc atttttgctt cttctgctcc tgtgaatccg
    15901 atatctaagc ggaccccgta ggcacccatc ttatggaaaa aagataagtc atcgtaagaa
    15961 atctccaact gttcaaataa cgctggattg atatcaacca ttacttccat gcctaggcta
    16021 ttcgcgtaat caactacttc tttaaattct gaaagaactt tttctttatc atcattgatc
    16081 tgcagcaaac tggtaaaaac ccgtttatac ccatacttat gagctaaatc caagtacgct
    16141 ttgtcctttt caaatgttga ccgttctgga taaatcgaga ttcctaattt tcccatatta
    16201 ccatttcctc ctgcatcttt tcgtttttgt atccacttac attatagcaa acacaaagta
    16261 aatggtctac accttttctt tttcttttaa aataaatgat aaattccaat aatttaagta
    16321 ggtattttcc ctctattttg ttatcattaa agataacaaa atagtaaatt ggaaaggaac
    16381 agttatgaag aaaaataaag ttattttaag tattagttta gcagcagcat tgctgtttgc
    16441 tgctggttgt tctgcaaaca acacgacttc ttctagttcg tctacagaaa gttcaaaaag
    16501 tacatctact gcggaaagca cgactgccag tagcgtcgat ttgaacgcat tggaattacc
    16561 acaattgacg aaagatgtcg ccgaaaatga agatctggtc gaaatggtga cgaataaagg
    16621 cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac
    16681 acatgcaaaa gacggttact acaacggact gactttccat cgcgtcatca aagatttcat
    16741 gatccaaggt ggcgatccta aaggcgatgg cactggaggg gaaagcattt ggggagaagg
    16801 atttgaaaca gagatcaaca accaccttta taatataaga ggtgcgttat ctatggctcg
    16861 ctcacaagat cctaacagta acggcagcca gttctttatc gttcaaaata cagatgatat
    16921 gcatgatggt cttttaaaag acgactatcc tcaagcaatc atcgacgctt ataaaaacgg
    16981 cggttatcct tcactagatg gccattatac tgtcttcggc caagtgattg aaggaatgga
    17041 tgtcgtggat gctattgcaa atatcgaaac agatagttca gacaaaccaa aagaagaagt
    17101 aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg
    17161 aatcctgcat agatgacaac gtatgctagg attcaggcgt cttcttttta cataaaaagc
    17221 aagtaagctt ccttttgttt tgctatatca ggttttatag tcaagcattt tggttgacga
    17281 tgaacgtgaa ctcgcactca cagacttttt tatcatctac atatgctgca gataacgctg
    17341 taccgactga tcccttcatt ttggtgattt caaaatgaca tttcagcaca tctcctggaa
    17401 caactttttg acggaatttg gccttattga tcccaccgat ataagctctt tttcctagaa
    17461 actcttctga ttttaggata agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg
    17521 cacccggcat cactggattg tcaggaaagt gaccttggaa aaaatcttca ttgattgtta
    17581 cattttttgt cgcaacgatc ttttttcctg gttctaaagt atctacataa tcaatataac
    17641 aaataggata acgattagga atcaattcca ttacttcagt agcagataat acgtaagaca
    17701 taattaaact ccttctttca ggcaattgcc attttcatta ctttgactat caaagta
//
LOCUS       DADVXN010000058        17502 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.060, whole genome shotgun sequence.
ACCESSION   DADVXN010000058 DADVXN010000000
VERSION     DADVXN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.060"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            104..454
                     /locus_tag="KB942_001691"
     CDS             104..454
                     /locus_tag="KB942_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBB6852115.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            553..1083
                     /locus_tag="KB942_001692"
     CDS             553..1083
                     /locus_tag="KB942_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6852116.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1237..2388
                     /locus_tag="KB942_001693"
     CDS             1237..2388
                     /locus_tag="KB942_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBB6852117.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2497..4536)
                     /locus_tag="KB942_001694"
     CDS             complement(2497..4536)
                     /locus_tag="KB942_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBB6852118.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4594..5151)
                     /locus_tag="KB942_001695"
                     /pseudo
     CDS             complement(4594..5151)
                     /locus_tag="KB942_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5690..5896)
                     /locus_tag="KB942_001696"
     CDS             complement(5690..5896)
                     /locus_tag="KB942_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852119.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5912..6526)
                     /locus_tag="KB942_001697"
     CDS             complement(5912..6526)
                     /locus_tag="KB942_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBB6852120.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6673..7356)
                     /locus_tag="KB942_001698"
     CDS             complement(6673..7356)
                     /locus_tag="KB942_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6852121.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7465..7923)
                     /locus_tag="KB942_001699"
     CDS             complement(7465..7923)
                     /locus_tag="KB942_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBB6852122.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8007..9770)
                     /locus_tag="KB942_001700"
     CDS             complement(8007..9770)
                     /locus_tag="KB942_001700"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBB6852123.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9991..11451)
                     /locus_tag="KB942_001701"
     CDS             complement(9991..11451)
                     /locus_tag="KB942_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852124.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11659..12585)
                     /gene="glsA"
                     /locus_tag="KB942_001702"
     CDS             complement(11659..12585)
                     /gene="glsA"
                     /locus_tag="KB942_001702"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBB6852125.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12608..14035)
                     /locus_tag="KB942_001703"
     CDS             complement(12608..14035)
                     /locus_tag="KB942_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6852126.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14066..15280)
                     /locus_tag="KB942_001704"
     CDS             complement(14066..15280)
                     /locus_tag="KB942_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBB6852127.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(15621..15863)
                     /locus_tag="KB942_001705"
     CDS             complement(15621..15863)
                     /locus_tag="KB942_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6852128.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(15895..16452)
                     /locus_tag="KB942_001706"
     CDS             complement(15895..16452)
                     /locus_tag="KB942_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6852129.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(16465..16653)
                     /locus_tag="KB942_001707"
     CDS             complement(16465..16653)
                     /locus_tag="KB942_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6852130.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(16666..17232)
                     /gene="amaP"
                     /locus_tag="KB942_001708"
     CDS             complement(16666..17232)
                     /gene="amaP"
                     /locus_tag="KB942_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6852131.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4985 a         3503 c         2972 g         6042 t
ORIGIN      
        1 tttgtcacaa tcctttttgt ttttttgaat aatcgctttt tcttctcttt ttcttatata
       61 ctataagtag taaataattt gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat
      121 ttttacgaca agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga
      181 tcgtataaaa gaggaagtag atgaaatcat ttgttggctg acgggttatt cccaaacaga
      241 actagaatat caactacaaa aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt
      301 ccccaatcct tcacgttcct tgattacagg tgttgtttgt ggtgtacgag tagaagacat
      361 cgaggataac ttgatgaaag aaattcgtta tttagataaa atgatcgatg agctagcaaa
      421 aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc cagagatatt cttctccttc
      481 gctcattttg taacatttaa tttggtaaaa tgactagttc acgttaaaat aagtcaaagg
      541 aggttttctt atatgtttga gacgattaaa caaaatattc aaaaatacgc cttattaaga
      601 gctgctgttt atattatttc tgggattgcg attgtgctaa acccttctgc tgtttttcat
      661 tttatcggtt atctgatcac cgcctatctt gttttgcttg ggttgttgaa tctttttgag
      721 gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg gtctgttcag tggtcttttt
      781 ttcctgatct tagctctgat cgtctatgct tttgcacctg caatcgtttc tatcctgccg
      841 attcttcttg gattagctgt tatcatcaac agcttgttcc agttgttctt cagcatgaat
      901 acaaaagcta aaagttggag tgtttatagt agtattctgt tgttgattgg cggattcgtc
      961 ttattgttca atccattcaa aagtttgatg cttctattcc aaatattcgg ctttattttg
     1021 attttcatgg gcatccagga gattatcaat ttcatcagaa taagaaaaat gactatacgt
     1081 taatcatttt tagttattaa aatagaccgt ataaaaacca acaatccaaa aaaatctttt
     1141 ggattgttgg tttttttgtg agtatatttt cctatttttt caataatgta tgttatgcta
     1201 agttcgggct atattttata caaaggaatg atgagaatgg aatttattgg tattttatgc
     1261 ctgatattga tttcaacaac gattggctca catatctcgc ggagattagg tatcccagca
     1321 gtgattggac aattacttgt tggtgttttg ctaggccaag ctggattgaa ttgggtcaaa
     1381 cctgatattc tagtccatga cttttcggag attggtgtgt tcttattaat gtttttagct
     1441 ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc gacctggtat gttcgttgct
     1501 ttgcttggga tcatcttccc ggttgttttt ggctggttga caggcaaagc attccaagtt
     1561 tcgggaaatg aagcgatttt ctttgggatt attcttgcgg caacttctgt cagtatttca
     1621 gtagaagtat taaaagaact aaacgtagtg aatacaaaag aaggatcaac aattttgggt
     1681 gcttctgtgg tagatgacat tttagtcgtt ttagttctaa gtttcagcct atcttttcta
     1741 acgggggaag cttctagcaa tgcttcttta ccgcttgttt tgattgaaca gctaatttat
     1801 tttgttttta ttttcttatt agtcaaatgg attgcgcctt tcctactttc atttgcagaa
     1861 aaagtgtacg caaattcttc tattatcatc gtatcgctag ttatttgttt aggaatgtct
     1921 tacgtagcgg atcttgttgg gcttagctct gtcattggtg ctttttttgc tgggatagct
     1981 gtcagtcaga caaaggttaa agaagaagta taccacaatg tagaggcttt aggatatgca
     2041 gtatttatac cagtattttt tgtcagtgta ggattagaag ttgatttttc tgactttagt
     2101 gaacaactac tctttatttc tatattgacg cttgtagcga ttttgaccaa attgcttgga
     2161 ggttattttg gtgctcagat tgccggtttt tctcaacata gttcattgat ggtcggtgct
     2221 ggtatgattt cgcgtggaga aatggctttg atcattttac aaattggaca gcaaagtcaa
     2281 ttgatcgaac cacagtacta ttcaccttta gttatcgtga ttttattaag tacacttatt
     2341 tctccacttg ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt aaatcaaaaa
     2401 gaagttgtga aagagacatc gtgaaaaccg tttatttgaa aaaaggactg tgacgataac
     2461 gccacagtcc ttttactgta cttaaaaagc aattttttat tgataatttt ggttgaggta
     2521 ttgcaatagt tccgatgctc gtttggttgc tgaatcatca tcatctttat tttccaacat
     2581 gctgaccatc atataacctt ggttatcaag attgaaagca aacaagaaac tgttctcttg
     2641 cccctttaca tcctgttttt ctttgatttc cgctgtacca gttttcgctg ccaacggaat
     2701 ccctaaagca gaaagagaat gtgctgtacc atttacatct tgcacaactt ctctcagatc
     2761 tggcacgatc gtttgtactg ctgtttcgct gatgacattc ttcttatctt ttgtctcttt
     2821 atctgcaatc aatttaggat agacaagtgt gccattgttg gtaaaaacag aatacattgc
     2881 tgcttgctgg ataggagtaa ttagaagttc gccctgtcca tatccagtat cagctagcaa
     2941 gatatctgag ttaaagctat cttcattaga aatttgtgct ggattcatac taattggcaa
     3001 atcaaggtct tcaccaaaaa tgaatttatc taagcctgta cgaaattttt tctcacccat
     3061 tttcaacgtt tcttgtgccg catatatatt atcggaatag atcaaagcag tttttaagtc
     3121 tacttgtgat acatcactac tgacacgcgt tgttttaaaa gatccccaag aactgtcttt
     3181 ttgccattta agcccgttga tcgtcaacac ttcatttgga tcgatagtgc cgttgtcgag
     3241 accgattgct gctgtaatta ttttaaacgt agatccagga gcataacctg tcgcaaatcg
     3301 gctgatgaat ggatgttcag gattttcttc ataagctttg taatcttctt gtgagatccc
     3361 gtttgtcatt ttgtttggat catagcttgg agagctagca agcgcaagaa gatcaccggt
     3421 ttttggcgtt gtcgcaacag ttgatccagc ttttcctcct agactgtcaa aagctgtttt
     3481 ttgtgccttc gcatcgattg tcaatttgat atctttgcca ttttgaactt catgttcgat
     3541 cagaactttt ttctctacac cgtctgcatc tgtaatgctt aatttctcac ctgttgtccc
     3601 acgaagatcc ttatcaaaag ccatctccaa acctgagcga ccgatttttc cattactgct
     3661 taattctgga ttcttatcaa tatcttctgc tgtgatatct cccacgtaac caatcagctg
     3721 agcagctgct tcacccaaag gataatatct gccgtctact tcttgaatgg tagctccagc
     3781 tggaagttct ggcgttgctc catcaatgat tttcaatggg acaaagtaat cgggttgtac
     3841 ccagctttgt gaaatcgcct gattgatagc atcttctgtt aagtcgaagg aggaagcaat
     3901 cgctttgata ttggctgttt tttcgcctcc atctccaagt ttacttggaa cgactcctaa
     3961 ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt cgatctataa tattccctct
     4021 tgctgcttct tgtgtggtca ggcttacttt gtcatttcct tccatttctg gaaaaaccaa
     4081 gttgggttgc cagttgatcg tggtttgacc atcatttctg tctaatgttc ctttataaga
     4141 aagatctttc aattcaccta atgaggtatt catctttgct ttatatgaaa aactgtaagt
     4201 agaatcgtct tttttatcta cttttagatt cgatatctga agtcctttga catcagcagc
     4261 accgtatata ttctggtatt gatctaagat ctctttttca gataatgcac ttttatttgc
     4321 cgcctttttt gatgtcattt ctgcagcttt gttataatct cccttgttca aagcttggac
     4381 aaattgctca actgtctttt ctccagcttc tactgcttgg gtttcttgat agtgctggta
     4441 gtaaaaataa ccgccacccg cagctgctat taagatcact gcgccggcaa tataagcgcc
     4501 tgttcgattt ttgccgtgct tgtcacttct tttcattttt tcctccttct cttgcttgtc
     4561 attcctttat ttcactattt atacctgttt attctaacat atgtctttga ggaaatccat
     4621 accacaatcc agaagcacac tttctggaag gttcgtttgg atactaccaa gtaatttccc
     4681 aaccgctttg ggtagcttga atattgaagt tacgtctttc agctgctgag agatagcttt
     4741 gatcacttgc tgctgccttc tgatcctccc gaagtctcct tctttgtctt cccgaaatcg
     4801 agcatactgg agtaaagtat taccatccat cacttgctct cctttgttta tatatacgcc
     4861 atctaaatct agagattttt ctgcatcgat cttgactcca ccaggaaaca gttcattaac
     4921 gcaatcgata aaatcttgaa atgtgatact tgcataatag ggagcttcaa atttgaaatt
     4981 ttcctttaat gtttgattta ataaatcaac tcccccataa gagtaagctg catttatctt
     5041 attcattccg taacctggaa tatcgacata tgaatctctc atgatagaga tcagttttgc
     5101 ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa tccgagcgac ctccaccaat
     5161 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     5221 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     5281 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     5341 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     5401 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     5461 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     5521 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     5581 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     5641 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     5701 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     5761 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     5821 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     5881 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     5941 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     6001 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     6061 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     6121 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     6181 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     6241 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     6301 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     6361 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     6421 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     6481 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     6541 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     6601 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     6661 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     6721 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     6781 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     6841 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     6901 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     6961 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     7021 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     7081 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     7141 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     7201 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     7261 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     7321 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     7381 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     7441 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     7501 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     7561 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     7621 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     7681 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     7741 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     7801 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     7861 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     7921 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     7981 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     8041 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     8101 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     8161 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     8221 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     8281 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     8341 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     8401 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     8461 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     8521 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     8581 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     8641 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     8701 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     8761 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     8821 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     8881 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     8941 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     9001 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     9061 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     9121 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     9181 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     9241 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     9301 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     9361 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     9421 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     9481 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     9541 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     9601 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     9661 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     9721 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     9781 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     9841 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     9901 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     9961 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
    10021 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
    10081 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
    10141 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
    10201 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
    10261 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
    10321 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
    10381 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
    10441 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
    10501 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
    10561 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
    10621 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
    10681 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
    10741 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
    10801 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
    10861 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
    10921 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
    10981 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
    11041 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
    11101 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
    11161 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
    11221 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
    11281 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
    11341 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
    11401 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
    11461 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
    11521 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
    11581 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
    11641 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
    11701 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
    11761 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
    11821 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
    11881 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
    11941 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
    12001 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
    12061 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
    12121 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
    12181 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
    12241 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
    12301 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
    12361 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
    12421 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
    12481 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
    12541 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
    12601 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
    12661 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
    12721 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
    12781 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
    12841 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
    12901 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
    12961 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
    13021 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
    13081 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
    13141 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
    13201 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
    13261 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
    13321 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
    13381 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
    13441 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
    13501 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
    13561 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
    13621 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
    13681 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
    13741 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
    13801 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
    13861 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    13921 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    13981 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    14041 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    14101 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    14161 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    14221 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    14281 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    14341 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    14401 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    14461 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    14521 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    14581 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    14641 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    14701 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    14761 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    14821 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    14881 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    14941 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    15001 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    15061 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    15121 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    15181 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    15241 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    15301 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    15361 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    15421 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    15481 tcttttttta tttttaaaga tattttttca taaggaataa agaatacttc atataaaata
    15541 tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt catttcaaca
    15601 taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac taatactaaa
    15661 atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg tccccaagtt
    15721 cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt tgcgatgatc
    15781 cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat accgccgacg
    15841 attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta cttcttattg
    15901 tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag cagattttgc
    15961 tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg ttactttgtc
    16021 ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg caacattcac
    16081 attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga tcaattcttt
    16141 gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat ctagatccac
    16201 agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat ctgtgttgac
    16261 taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac catctacatc
    16321 tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca attcaccatt
    16381 catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg tattcatgtt
    16441 tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta ttcctgactg
    16501 tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca acaatgtctt
    16561 gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc caaagataat
    16621 agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt ccactcgaga
    16681 gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga ctgccacttt
    16741 gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt gcatcaatac
    16801 acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat ggatatggat
    16861 tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt cttcgtgtaa
    16921 atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc catcttcccg
    16981 tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca agatgataat
    17041 agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt agtaattcgt
    17101 taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg cattgatcaa
    17161 cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga ttatttttaa
    17221 ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata actctaccga
    17281 caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc gctaccaaac
    17341 attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca attgcttact
    17401 tattctctat ttacttttga ttgctggcta tacttaaagg atattattca agattaaaaa
    17461 ttgcaaagat tatgctcagt tattattaca agattctatt ta
//
LOCUS       DADVXN010000059        17264 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.061, whole genome shotgun sequence.
ACCESSION   DADVXN010000059 DADVXN010000000
VERSION     DADVXN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.061"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(18..563)
                     /locus_tag="KB942_001709"
     CDS             complement(18..563)
                     /locus_tag="KB942_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6852132.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(575..1267)
                     /locus_tag="KB942_001710"
     CDS             complement(575..1267)
                     /locus_tag="KB942_001710"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBB6852133.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1286..1570)
                     /locus_tag="KB942_001711"
     CDS             complement(1286..1570)
                     /locus_tag="KB942_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852134.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1583..2146)
                     /locus_tag="KB942_001712"
     CDS             complement(1583..2146)
                     /locus_tag="KB942_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6852135.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2374..3036
                     /locus_tag="KB942_001713"
     CDS             2374..3036
                     /locus_tag="KB942_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBB6852136.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3160..4356
                     /locus_tag="KB942_001714"
     CDS             3160..4356
                     /locus_tag="KB942_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBB6852137.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4457..5833)
                     /locus_tag="KB942_001715"
     CDS             complement(4457..5833)
                     /locus_tag="KB942_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6852138.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5870..6737)
                     /locus_tag="KB942_001716"
                     /pseudo
     CDS             complement(5870..6737)
                     /locus_tag="KB942_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6826..7851)
                     /locus_tag="KB942_001717"
     CDS             complement(6826..7851)
                     /locus_tag="KB942_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6852139.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7869..8543)
                     /locus_tag="KB942_001718"
     CDS             complement(7869..8543)
                     /locus_tag="KB942_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6852140.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8727..9620)
                     /locus_tag="KB942_001719"
     CDS             complement(8727..9620)
                     /locus_tag="KB942_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6852141.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9694..11391)
                     /locus_tag="KB942_001720"
     CDS             complement(9694..11391)
                     /locus_tag="KB942_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6852142.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11384..12697)
                     /locus_tag="KB942_001721"
     CDS             complement(11384..12697)
                     /locus_tag="KB942_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6852143.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12697..16734)
                     /locus_tag="KB942_001722"
     CDS             complement(12697..16734)
                     /locus_tag="KB942_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6852144.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16747..17205)
                     /locus_tag="KB942_001723"
     CDS             complement(16747..17205)
                     /locus_tag="KB942_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6852145.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4836 a         3889 c         3010 g         5529 t
ORIGIN      
        1 caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat
       61 gcccattcat gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg
      121 tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg
      181 gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta
      241 aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg
      301 tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga
      361 ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa
      421 acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt
      481 acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag
      541 tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga
      601 tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg
      661 ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac
      721 gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg
      781 ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc
      841 cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg
      901 tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg
      961 ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc
     1021 cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga
     1081 tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca
     1141 cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg
     1201 gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga
     1261 ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata
     1321 acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt
     1381 cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa
     1441 agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta
     1501 atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc
     1561 ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg
     1621 cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa
     1681 gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct
     1741 tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat
     1801 acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc
     1861 tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct
     1921 ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt
     1981 cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc
     2041 ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt
     2101 ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt
     2161 tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta
     2221 gtttaccata gataaacctt agattttctc taaatataga aaatccttct ttagtccgat
     2281 actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca
     2341 atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt
     2401 ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga
     2461 cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag
     2521 ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc
     2581 ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag
     2641 atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc
     2701 catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga
     2761 ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat
     2821 aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa
     2881 gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc
     2941 gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag
     3001 cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact
     3061 cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat
     3121 gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa
     3181 acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca
     3241 actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa
     3301 cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag
     3361 ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc
     3421 caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt
     3481 ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac
     3541 gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg
     3601 atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg
     3661 aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac
     3721 cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa
     3781 atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc
     3841 gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa
     3901 cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt
     3961 ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc
     4021 attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca
     4081 gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa
     4141 atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc
     4201 gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc
     4261 caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa
     4321 gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa
     4381 caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta
     4441 acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat
     4501 aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac
     4561 gaagcatcaa tacaatacaa gtcaaaatgg agacgattgg caatagcgga acgaatggca
     4621 ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac
     4681 taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg
     4741 ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg
     4801 tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag
     4861 caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa
     4921 ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag
     4981 ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca
     5041 cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt
     5101 tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa
     5161 aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc
     5221 agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt
     5281 tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt
     5341 aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat
     5401 tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca
     5461 tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca
     5521 accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc
     5581 caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa
     5641 cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa
     5701 cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat
     5761 cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac
     5821 gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt
     5881 tcatataccc taaaagggat accacctaaa atgactaaaa ggcaaccaac aataacataa
     5941 atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata
     6001 atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga
     6061 agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac
     6121 tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt
     6181 tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca
     6241 ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta
     6301 ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca
     6361 agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag
     6421 aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct
     6481 acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct
     6541 aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa
     6601 aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca
     6661 tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat agggactaat
     6721 gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg
     6781 aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt
     6841 ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc
     6901 agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc
     6961 tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg
     7021 tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg
     7081 cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt
     7141 actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc
     7201 ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat
     7261 cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact
     7321 gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt
     7381 ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt
     7441 agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc
     7501 ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat
     7561 atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg
     7621 ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc
     7681 cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa
     7741 atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag
     7801 ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc
     7861 tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata tccttggata
     7921 cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct
     7981 acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca
     8041 ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct
     8101 ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt
     8161 tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc
     8221 agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg
     8281 atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca
     8341 cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca
     8401 agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag
     8461 gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct
     8521 tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata
     8581 tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt
     8641 aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta
     8701 ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt
     8761 ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg
     8821 agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct
     8881 tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt
     8941 gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt
     9001 gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca
     9061 agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca
     9121 ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg
     9181 ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc
     9241 aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct
     9301 tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt
     9361 cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt
     9421 tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt
     9481 tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg
     9541 gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta
     9601 ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt
     9661 ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt
     9721 acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag
     9781 cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc
     9841 tggattttct actcctacga tctgaatttc ttcattatag cttccataag agatgataaa
     9901 ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt
     9961 catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac
    10021 aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac
    10081 agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa
    10141 ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct
    10201 tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt
    10261 tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga
    10321 aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa
    10381 agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct
    10441 tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc
    10501 tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt
    10561 gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat
    10621 cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc
    10681 agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc
    10741 tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca
    10801 tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac
    10861 cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac
    10921 tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc
    10981 agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc
    11041 tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc
    11101 cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc
    11161 aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg
    11221 tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg
    11281 tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc ctattcccgc
    11341 agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc
    11401 cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct agtaaagaaa
    11461 tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat tgacctggat
    11521 tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa
    11581 cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct aattcagcca
    11641 tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc caaaattcag
    11701 ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc acacctttta
    11761 attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta acaaggccct
    11821 ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg tctgctggtt
    11881 tgatccattg atttttcaag tcattagcag gagccatatc taaataatat ttcccccctt
    11941 ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt tcagttgttc
    12001 ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca tttttaggat
    12061 agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt ggcaaaatag
    12121 cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct ggatcaatcg
    12181 attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt gctttgatac
    12241 gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc gaagtcccta
    12301 tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc gtttcagcga
    12361 tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca tgataataat
    12421 cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt gaaatgatat
    12481 ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg tattcaaatt
    12541 gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc acgacatctt
    12601 cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc ggtagtaaaa
    12661 caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct cctttttaga
    12721 ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag
    12781 gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag gatcgttccc
    12841 gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta actcttgctg
    12901 tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt ttagtgtacc
    12961 cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc gataactaat
    13021 ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca cttccttgat
    13081 ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt ttggatctgg
    13141 tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct caattgaaaa
    13201 ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat
    13261 tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt catagccttc
    13321 cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact taccttcttc
    13381 attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg cgacagattc
    13441 cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta aatcgacata
    13501 cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac gtttcgattc
    13561 ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag aatcccccac
    13621 tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta attttctaac
    13681 tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata aacgatcctc
    13741 ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct tgagttggat
    13801 agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat caggttcttt
    13861 gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg tagtttcttc
    13921 actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt tctcaccatc
    13981 cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc ctttctcttc
    14041 ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt ccagtccgtt
    14101 accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac tgccttcaat
    14161 tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca gtttgacata
    14221 caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa gcttgtgata
    14281 ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc tcgaaccaga
    14341 tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg ctgcatattt
    14401 tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc cagcgatctc
    14461 tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg gcatcttcgt
    14521 ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt cggcttcccc
    14581 actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct cgccattttg
    14641 acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg aagaagatgg
    14701 gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct gaatataggc
    14761 catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt ttacttggta
    14821 cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat aagcttggtt
    14881 ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt gtccatgacg
    14941 ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg gatctagatg
    15001 gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa aaccagtttc
    15061 atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat cctccaaggc
    15121 tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc
    15181 taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt gtctcccgct
    15241 aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct cttgatactt
    15301 gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt cttgcgaagt
    15361 aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag tcgctttatt
    15421 atctccttct tttacctctc cataacgata gtgtacattt acttcatctt ctggacggtt
    15481 aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat tcccagaaaa
    15541 ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt ccatcacagt
    15601 ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg gtgcataact
    15661 gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc tattttctcc
    15721 gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa gcggagttcc
    15781 tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat tgatatcatt
    15841 tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg tactattatg
    15901 gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca gatctttact
    15961 catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg tcccagcggt
    16021 taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg gactgatttc
    16081 ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg catcttcagg
    16141 atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt tgatgtacgc
    16201 cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa gctgaacggt
    16261 ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag tagcatagtc
    16321 gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca agttctctcc
    16381 gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc aatttcctaa
    16441 cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt tttcaacttc
    16501 tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa gacgctccaa
    16561 aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg
    16621 ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg aatcttctgt
    16681 atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca ccacttgaag
    16741 aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca atgtgccggt
    16801 ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga tcgtctcgac
    16861 ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa cgttttttac
    16921 ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt cattggcact
    16981 ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa tatacttccc
    17041 ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt ctgttgactc
    17101 agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc caaatagcaa
    17161 actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct tatactcctt
    17221 tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgc
//
LOCUS       DADVXN010000060        17020 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.062, whole genome shotgun sequence.
ACCESSION   DADVXN010000060 DADVXN010000000
VERSION     DADVXN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.062"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(351..965)
                     /locus_tag="KB942_001724"
     CDS             complement(351..965)
                     /locus_tag="KB942_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6852146.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1571..1948)
                     /locus_tag="KB942_001725"
     CDS             complement(1571..1948)
                     /locus_tag="KB942_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852147.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2248..3129)
                     /gene="galU"
                     /locus_tag="KB942_001726"
     CDS             complement(2248..3129)
                     /gene="galU"
                     /locus_tag="KB942_001726"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6852148.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3172..4191)
                     /locus_tag="KB942_001727"
     CDS             complement(3172..4191)
                     /locus_tag="KB942_001727"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6852149.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4824..5828)
                     /locus_tag="KB942_001728"
     CDS             complement(4824..5828)
                     /locus_tag="KB942_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6852150.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5841..6890)
                     /locus_tag="KB942_001729"
     CDS             complement(5841..6890)
                     /locus_tag="KB942_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6852151.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6907..7728)
                     /locus_tag="KB942_001730"
     CDS             complement(6907..7728)
                     /locus_tag="KB942_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6852152.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7715..8494)
                     /locus_tag="KB942_001731"
     CDS             complement(7715..8494)
                     /locus_tag="KB942_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852153.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8511..8981)
                     /locus_tag="KB942_001732"
     CDS             complement(8511..8981)
                     /locus_tag="KB942_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852154.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8993..9412)
                     /locus_tag="KB942_001733"
     CDS             complement(8993..9412)
                     /locus_tag="KB942_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBB6852155.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9493..12270)
                     /locus_tag="KB942_001734"
     CDS             complement(9493..12270)
                     /locus_tag="KB942_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6852156.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12461..12685
                     /locus_tag="KB942_001735"
     CDS             12461..12685
                     /locus_tag="KB942_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852157.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13464..15179
                     /locus_tag="KB942_001736"
     CDS             13464..15179
                     /locus_tag="KB942_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852158.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            15182..16945
                     /locus_tag="KB942_001737"
     CDS             15182..16945
                     /locus_tag="KB942_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852159.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         5025 a         3492 c         2896 g         5607 t
ORIGIN      
        1 tgtactataa ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag
       61 agacaagaaa gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt
      121 ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa
      181 caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca
      241 aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat
      301 ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca
      361 cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc
      421 gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca
      481 tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca
      541 ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga
      601 tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc
      661 gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct
      721 gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca
      781 agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat
      841 atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc
      901 agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa
      961 ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa
     1021 ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac
     1081 acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc
     1141 agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat
     1201 taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact
     1261 ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt
     1321 ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa
     1381 aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc ttatcccgat
     1441 acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct tttttccacc
     1501 aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt ttgcgtatac
     1561 ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag
     1621 aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga
     1681 tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac
     1741 ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa
     1801 tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata
     1861 gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg
     1921 actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta taattaaaaa
     1981 attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc ttatgtaatc
     2041 attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt tctgaatcta
     2101 ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta gagtcaaaaa
     2161 actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca aatagccttt
     2221 cattctcagc aggccactta tttccagtta actttctgtt cttagctttt tactcaactg
     2281 gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat attcaatatt
     2341 tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa actctctcgc
     2401 atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta tctcgtttcc
     2461 tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta gataacgtcc
     2521 gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa acccttttac
     2581 tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt tagatgtttc
     2641 attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat aatccatcat
     2701 caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca tgacgacaaa
     2761 cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc ctcttggatg
     2821 agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag cgagtaattc
     2881 tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa agtgatcttc
     2941 aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg aatccaatgc
     3001 ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt cttttgccat
     3061 ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga ctgctttttt
     3121 gaccttcact tgctgttcct cctaaacgac gattaactat tgatcatcta tttaacgaag
     3181 agaaaattca ttctccattt ttccgtcacg taacatgatt tcttttgctg cttttttgat
     3241 atcttctcca ttatacaaaa cattatagat tgtttgagta attggcatct caacacctag
     3301 ctgttgagct agctctactg ctgcttttgt cgttgccaca ccctcaacga ccattcccat
     3361 gttttctaga acttcttcca gcttatgtcc ctgtcctaac aagttaccgg cacgccaatt
     3421 gcgagaatga acacttgtac aagtaacgat caagtccccc acaccactca aaccgataaa
     3481 tgtcagagga ttggcaccca tcgcgacacc aagacggctg atttctgcca atcctcgagt
     3541 catgattgct gcttttgcat catctccgaa tcctaatcca tggatagcgc cggcaccaat
     3601 tgcaatgata tttttcagtg ctgcaccagt ttctacgcca atcacatcat tatttgtata
     3661 aatcctgaaa taatcattca tgaacagttt ttgtacatag gctgctgctt tttgatcaat
     3721 acttgcagct gtaattgttg tgatatcatg tactgccact tcctcagcat ggctaggacc
     3781 agataataca acaattgcct gtcttttttc ttctggaatt tcttcagcca aaacttctga
     3841 gattcgttta tgcgtatctt gttctagtcc tttacttgca tgtataatga ttggcttagt
     3901 attcatttta ccaataagtt cctgtgcgac tgaacgaatc gcttttgttg gaacaacaaa
     3961 taaaatagca tctgcgttat ccactgcttc tgccaattct ttatatccga taatcgattc
     4021 tgggatcgtc aaatcaggta aaaaatgctg atttgtatga tacttattga tttcatcaat
     4081 ctgttcaggt acatttcccc aaatacggac atcatgcccg ttttctgcca atgtttgtgc
     4141 taaagcagtg ccccaagatc caggaccaag aactgcgatt ttttgtttca tggatattcc
     4201 tccaaataaa taattgataa tttccattat agtatacatt tcttaaagtt gggactaatt
     4261 tccattgttt ttaagtgttt gtttacttct tttatcgttt ttacttgaaa taatcaatga
     4321 tatgttacaa tagtctacat tttattttta cactcgttat gctttgcttg ttcctaagag
     4381 gaagcagttt tgacaatgtt actaccagca ttactattag gaggttagga aacttctcct
     4441 agtgagtatt gatttaaaat acataagaga cagattgaac atttctaaat ctgtcaggtc
     4501 ctgcaacgga cagattgtct ctgttgacgg ttgacatagg aacttttgtt ttagtcaaca
     4561 ttctggatac aaaacgctat gtaaactggc gcaactcgtt gtacttggac ttttactacg
     4621 gatccatgca acaactaaat agtactattg cataacaaaa aatggttggc acccacatgt
     4681 cccattaaac agattataag ttgaactgta gataatgcaa aaaatgtgtt ttataacgta
     4741 attgatgcca atattttaaa aaaataaagg gagcagttat cgctgctctc tttatttttt
     4801 atattctttt tatccagtct atcctaataa tcaaactgac gatagtagcg gcgtatctct
     4861 aatggatggc agttcaactg ctccacatga gcatcgatgc gttgtgtcac tacatgcatc
     4921 aataagaaag acaaaacacg tccgcgatat ttttcactga taccttctac tggatagtct
     4981 tttgtgtcta tcaatgtata attgctgcaa atttttggca aaagtttagc cacacgttca
     5041 gataacactc tctgttcatc ttcgcctaaa aataatgtga ctggggttgt ttcttctacg
     5101 atctctaacg tcccgtgaag aaactcggca gcatggatag atttcgatgg aagccagctt
     5161 tgttcttccc agtaacacat ggcatatgaa taaacagctc cccattgatt ccctgctcca
     5221 ataaaataat gcatactatc acgtcgatgt ttttctgcaa atgataagcc aaatgcatct
     5281 gcttttttct ctgcttccac taaccctgtt ggtaaataac gttcgagctg ttcatagtat
     5341 tctgaataag cttcgaattc tccattcaaa tacatcaatc gatctgctgc cataaaaaat
     5401 ttgatttgtt ctgttccagg agctggataa gtgaccacaa aatcgcatag ctgagacaac
     5461 ttactgttcg ctttatcgat aaatccaatc agtgtcgctc ctactttctt gatttgttct
     5521 actgctttca ctacttcttg agttgtaccg gtaacagagg acaaaataac gatagaatct
     5581 tttgttaatc ttttgtttcc agtagtgtaa tattctgcag catgctgtac aaagacaggc
     5641 aagttgcttt tcccattcat ataagtgaca gcttgcatcg aggaagcata cgttccaccg
     5701 attcctagat aatagacagc gtcaaatttt tccgcataaa gtttatcgat gatttcttct
     5761 acttgcggac gtaaagctag tgcgcctttt attgctttga tttgttcttc ttcattgaat
     5821 ttcagcatga tttccacctc ttataattta cttttcaaga ctgtatcaaa gtcttcaaaa
     5881 atctttttgt tcaagtcaat acctttagct actgcggttt tataagcgaa tagttgaaac
     5941 ggaacgacaa gcgctaatac agacagatat tcatcacatt ctattgctaa tccaagtgtt
     6001 ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct ctccaacgta ccgactcata
     6061 tatctttgca acttttgcga acgttcctta tttacacttt ccccttcaat aaagaatagc
     6121 aaatgcgaag catctgcctc caggtatggg ccgtgcatat aagcttctaa ctcaaatcct
     6181 tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg cttctttagc tgttccccag
     6241 tttggaccat atccaatggt gacgaaacgc tgtgctaaac gaaataaact ttgatatcca
     6301 tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat ggttgattat tttgcgcaac
     6361 tgttgcatgt agtcttgttc tatttttttg ctgattttat ttttacttat tccaattcct
     6421 atccctaaga gtagtaactg taaaacagtc gcagaatagc cttttgtaac gaagcctact
     6481 gattctaccc cggcacctaa atcaataatt tgtgcagctt ctaaagcaag tggactttgc
     6541 acgtcatttg tgatagcaac aacaggtaac ccttgttttt gtatcatttt tactgcttca
     6601 acagtagaag cacttttccc gctttgtgac acagcaatca cagtatcgat tgaaggagaa
     6661 agttttccat aattgaaaaa ataaaaaggt tcttgaatat cgacagtaag atcaccataa
     6721 gattccaatg ctggcttcgc tgctaaacaa gcgttataag atgaacccgt tgctaagatc
     6781 agtaaattca ctgttttttt catatgttct cttgtcgaaa aatcattctt tttcaagatc
     6841 gtacttagta cttccttttc ttcatagata taatcttgca tcgtaaacat tttctctgct
     6901 ccttttttaa aaaatcccta tccacgaacc aaaaattcca aagaaagcta gtccgatcaa
     6961 gatcgttaat ggtttcactt ctttcttcaa tagataaaac actatgccaa atgctccaag
     7021 acataagata tttggaacga tatcatcaaa aatactttga acagtcacag cattttctcc
     7081 tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg gcagtcattg ctccaacgac
     7141 catcagtcca ataatagacg cgcctaatgt caaactttcc atcataccgt ttgcctggat
     7201 ctttttcaaa aaacccgttc ctaagcgata tccccaacct gttgctagat agcgaaacag
     7261 aatgtgcgga atattgaaaa tcaaaatgaa caagataggg cctaaaatat ttccttgcat
     7321 ggctaaagag gtacccacac ctgtggcaat caatcgcaaa gttccccaaa agaaagaatc
     7381 tcctaaccct gcaagaggtc ccatcaaaga tgttttgata ctatcgatcg cagtcacatc
     7441 aaattcagga tcatccttat tttcttcctc cattgcagcc gtgattccta aaatcagtgt
     7501 aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt ttcaaagctg ctgccatttc
     7561 ttctttcgac tgatataact ttttcaaaat cggaatcatt gcataaacat aagccatatt
     7621 catctgacgt tcataattcc aagaaaactc catttgaaaa gaacgccaaa atacgtgatt
     7681 tagctctttt ttcgttattt tgctgttttt gatctcagaa ttcttcatct tcttctacct
     7741 cccctgcagt tgtttgaagt ggtagttgac ggacattcat aatattgact accacaactg
     7801 ctaaaatcgc accaaaaata gctactccgg tcacaggaat ctctaagtaa gaagctatag
     7861 caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat cagcaaacga gcaagcatcg
     7921 caaaaccaag agctggaatc aaccctgtag ctactgacaa ccccttttga acgaagtcag
     7981 gaatcatatt taatagattt ccgatcgtat tgcttcccac catataagaa accattacca
     8041 caacaccaag catcaaggaa agaccgatac cggcaagcaa gtgcatatgc tcgactcctt
     8101 tataatttcc ttcttctgca taattgtctg ctttatggtt cataattgga atcaaaagcc
     8161 ctaagtaaat atttttcaat atcaatgtca acgtagcaat cggcaaacct agtaaaagcg
     8221 cggtttctgt accggctcca gctgtaatag caaaagccgt ccctaaaatc ccaccggtca
     8281 caacatcagg tggaatcgaa gcacctactg agaacgatcc gataaatgct aactctaaag
     8341 ttgcgcccat aatgatccca gtttttacat cacctaacac gattcctgta aacagccccg
     8401 tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata ttctgattgt gcgacaaatg
     8461 caacaattcc taataagact gcttgtacaa tcattgaggt tcttcctttc ttacaaaact
     8521 tctcttacat ccactttttt atctgctggt acttgacgga tttctacttc tatttgtttg
     8581 tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa tatttaccgc ttttccgatg
     8641 ctgcgagtac caggttttgc cttgatccct cctaagttga tggttttgat ttctggatac
     8701 gcttctgcca gcttatatgc atcctcgatc gattccacta cgatgaataa cttatattta
     8761 tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa ttgatttgat cactagtttg
     8821 actccttgtg gcttggctaa tttgatcgtt gtctttcgta tctcattcgt aggtacatca
     8881 tcattagcaa tcaaaataca gtctgcgcct attgtttgtg tccatgaaaa agctacttgt
     8941 ccgtgtaata accgatggtc gactcttgtc aatacaatca tttttcttcc tcctaaaatt
     9001 cgtcttcttc tattttctga ttgagccgct cgttacaaaa ctggatcatt ttttttgaat
     9061 cagttagcac ctgctggatc aaaacagttg tctctacttc agcgtccaac cgagatacta
     9121 gctcaatcaa tagtggcaaa ttcaatccgg caacgagata gacatgatcc cgattgatat
     9181 attgtaagaa ttcgttgttg acacttccgc caaaaagatc cgtcagaaca atgatttctt
     9241 tatctttgta cctcataaaa agctgcctta ctgtttccgc taaatcaaaa ttctcatcag
     9301 tatagcaatc gaatacagta atagaatgat ttttcccata gatcagctct aatgaatcta
     9361 atatccctga agcaaatttt ccatgggaag ctaagataat ttctctatcc actttttcat
     9421 ctcctctttt cacttacaca tactaaaaaa gcaagttgca tgccaaaatt agtggcaaca
     9481 acttgcttat gttcaaaatt cttcttctat cgagagaaac tctgtatctt cactcaaaat
     9541 atcatagata taggcaatct cagaattggg gatcatgaca ctataatgct cttcaatgac
     9601 actaaacgca gatttaatag cagataaccc ttctttttga cactgcttcc atttctctaa
     9661 tccttgatag gtttcgattg gcatttgtcg gatcaatcgt tcaatgagac agctcacatg
     9721 gacatacaac gctaattttt tgggattgct gatgcttcga ttcaactgca cctctaattc
     9781 tctcataaaa tgatcgattt ctcccatcac cttctctgta tctagaatag tcacggaatc
     9841 cagtactttt tccaatgaaa aattacgaat gatgcgctga ttgaatattt ccttctctga
     9901 agcattcata gtagtgtcca accattcagc tagtacagac atcttttccc ccgagatgag
     9961 gtcttctaat gaaagatagt cgatattggt gatatcagga tctgtggtgc caatcattcc
    10021 tacgacttcg tatatggaaa ataatgggtc actgtttttc ttatctttta attgctgata
    10081 ttcatatggg atgattttca actgacaagc cgtaggtaag cttttttcta gcagttcact
    10141 taagtggacc gccgtaccga ttccagtgaa gcaagtagta atgagtgctt tgggtttgtt
    10201 ttcctctgga tagagcagtc gccattctgg ctggatctgt cgaacagtct cttctgctat
    10261 ctcttctaat gatacatttt tttgcaggca ttcccctact gtgattgcta aaggtgtcgt
    10321 cacattattc aaaatcaaaa gcggtacgga taactgtttt ttaaaaaact gatggatttc
    10381 tttcagagat cccatgtcta ctaatatgat caatccattt gaaacatcgc tgttttcgca
    10441 ataatcaagg atttctgaag caatctgctg tggtgtgaca tctagtggca tatcaaatga
    10501 ttcaaataga tcctcttgta ataaacgatt tactacattt gccatactgc tagcagttgc
    10561 ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt tcttttgacc atttcgcttt
    10621 ttttagatag atcgtcagta aaatccgatc catacttgtg tcttcaatat ctaatttgat
    10681 cttacttaat tccagaattc gttggacata gtgataacta gatggataag actgagaaac
    10741 ttgttgttct aattctttga ttagttggat aatatcatgt tcttccggta accatttgat
    10801 agtgcttcgc tgatagagat agtaactcaa ggcatagaca ctgtttccat cgaatttgat
    10861 ttgataggca gtttccattt gtcgaaaggt ctctcggata gattgtgtcc aataaaccag
    10921 cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa aaatcaaata attgaatcac
    10981 ttcttgcttt aattttcctt cgcaagaata tagcagacag ttatttttct tgaaatcaat
    11041 taggatttgc tcaaaacttt taacgattcg ctgctgttcg ggctgttgct tctccattaa
    11101 ttgctctaag gtctggtctt gcgtaatata gattccttgc tgtgctgtat ttttagtaat
    11161 aggctgcgca agtagttctt tttgcaaatg atggatcgtg acatggattt caggctgttc
    11221 acgctgttca gcatatgctt ttgctgctga aactttgacg atatttttca gctctcctat
    11281 gtttccggat aatttctgac ttgttaaaag tgacaaagct ggtccactga ctgacaaagg
    11341 gctccccatt tttctttgtt cttcgagaaa aaaagagtaa atcagttcta gccgttcatt
    11401 acgactacgc tgattaagtg caggtagttc gattcggata ggtattcttc gaatgaaagt
    11461 tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa aagagacgtg ttttgatccg
    11521 gcgtgacaaa ttaggttctc ccatacgata aatcacacct tgatcaagat aggtgaacag
    11581 tttttcctga ccttctggtg ttaagcgatg tacctcgtct aaaaacaaga tcccaccatc
    11641 tgctgcttca aaagcccctt ttttattttc ttctgcacca gtgtacgctc ccttaacatg
    11701 tccaaacaaa ttgctagtca aaagttcagg attatctgca tattgtgcac aattaaaagt
    11761 gatgaatggt gcagaatctt ctagcagatc atgaagcaga caatactgat agaccaaatt
    11821 aactaaataa cttttacctg tgccactttc gcctgtaatc aaaagtggta atccgccatc
    11881 tggatagtaa agagctgttt tgatttgttc gatactttct ttcaaactgg cgtcgtatcc
    11941 gatcataaaa gagaataagt cttttggttg cgcgaaaaaa ggctgttcct ctttgagatc
    12001 atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat tgtttttcaa aagcttcttt
    12061 gtggaaataa atcactggtc gagaattgat tttgactagt agttgctcat catttaactg
    12121 gttcaaataa tgactgactg tgtttctttt taccccaaaa tgatcagcta acctttgtgc
    12181 tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct gtctggttct ctaagtaaca
    12241 taaaatttcc ttcttcaaca acttattcac ccctcctcca tcatagaaaa cggattcatt
    12301 ttgtcaataa aatctttaat aaagaattga aaaatatatc ttattataga cagcagccta
    12361 cttatccctt ataattttta ttctaaaaaa cgtacagttc cttccagttt ttatactgga
    12421 atttcaacat atgaaagaat aaggaggcta tgactattca atgaaatcgg ataaatataa
    12481 aaacaggaaa catacttcct ggaaaacttt tcgagaaatc atcggatctt atctcgctgt
    12541 agttgtattt ttgcctttgc tttatatggc tatgacagaa caaactcgtt ttattggcgt
    12601 agatacgcaa tatatagccg aaacaggatt tttattcatc tttatcttga tttttgcgtt
    12661 ctataaatcc ttccatacaa aataagattc acagatttca actttcctgt ctgtttttag
    12721 ctattacaaa tccttatatg tggtataaca acaatttatt ggcataatga ggaaaaacga
    12781 gttatgatgc tttcagagag taaagaggta ctgttgtcac catccttttt attgattacg
    12841 tacaccactc ttagtttttt cccattgatt acgaaaacaa ggtagaaaca ttgatgttcc
    12901 taccttgttt tcttttttta ttttcagatc aaaatctcaa gtggcttttg acttgatgtc
    12961 gggaggttgt cttcgttcca tcaaacctga tagaatctgc gccaaaccat agttttctcc
    13021 actattattt attcctctaa gatagtaccc acaataaacg aataaatatt tttctcattt
    13081 tcatactcct tctttgaaaa tcaattagat ttctctcaac ccactttttt acaaaaatta
    13141 cgtagcaaaa aagattgcaa gaacaaatta aaaaagaagt aaatagagtt tcatctataa
    13201 acatttactt gatatatttt ttatgcagaa ttcatttata gaaaaattaa gtaaaataga
    13261 cgttcctatg aatgaagaga ctggcatgtt ttctcatttt ctttttttta aaaaaaatag
    13321 tctattcaat ttctaaagct tcttcataat aaaactttca tttatgaaaa ggcttgttct
    13381 cttttcattc taaatttcct aagctacaat gtggtagtac ttgttttttc aattgtggac
    13441 aaataaataa taggaggaat catatgattc agttattacg ttatgcaaaa gactatcgta
    13501 agcaaatcat tttgggcccg ttcttcaaat ttcttgaagc tgtcttcgaa cttgtcctgc
    13561 ctttaatgat ggcttccttg atcgataatg gactaaaaat gaatgaccga ggaaaaatca
    13621 ttgaaatggg cttatggatg gtcgctatgt ctgtgattgg tctgatttgt gctatcattt
    13681 gccaatacta tgcttcagta gcttcacaag gatttggtac agagttacgt aatcagctaa
    13741 tcaaaaaaat caatactttc tctcacaacg agttgaatca ttttggaaca gataccttga
    13801 tcacccgaat gacaaacgac atcaatcagc ttcagctagc tttagcaatg gtcattcgtt
    13861 tattgatcag agcaccgttt ttaagtatcg ggtcagtggt catggctttt gtgatcgact
    13921 gggaagtagg tctgtttttc ttagcattat tgcctatttt ctcaatcatt ttatttttta
    13981 tcatcaaaaa aaccgttcct ctctaccaaa aagtgcaaga aaaactagat cagctcaatg
    14041 aacacgttag tcagaatctt agcggtgttc gtgtcatccg cgcatttgcg cagacaaaaa
    14101 aagaaacaga gaagttcgat aaaagcacaa atgatctagc gaacaattat ttacgtgttt
    14161 ccaatatctc tgccctatta tcacctgcta caaccttgat catgaatgtc gggatcatct
    14221 gtttgctgac agttggcggg atcaaagtaa atattggtag cttgcaacaa ggacaagttc
    14281 ttgctttgat caattatatg aatcaaatgc ttttggcact gatcgttgtt tcaaacctcg
    14341 tcgtgatctt cacacgtgcc gaagcttcag gaaatcgggt gaaagaggtg ctagacacag
    14401 aaaattcgat tcttgaagct gaggtcagta ctactcccga tttttcttct gaagcaatta
    14461 tcgcttttga ccatgttgac ttccgttata ctcctgattc tggtctttct ctccaaaata
    14521 ttacatttca gctgaagcga aatacagtcc ttggaatcat tggtccaacc ggaagcggga
    14581 aaacgacttt gacacaattg attccgcgtt tctatgatgt cagttccggt acagttataa
    14641 tggacggaat ggatgttcgt tcttggcagc tggatcatct ccgtcagcaa attgctcttg
    14701 ttccccaaac ttctgtacta tttaccggga ctattcgtga aaaccttcaa tggggtaaga
    14761 aagacgccac agaaaaagaa tgttgggaag ctctgaaaat cgctcaggca gaagaatttg
    14821 tccgtcagct tccaaatggt ttagatacaa aagtcatgga aaatgggaaa aatttctctg
    14881 gcggacaaaa acagcgactg acgattgcaa gagctttgat tgccaagcca gcattactga
    14941 ttttagatga ctctttaagt gcgttggatt atcaaacaga cttggatttg cgtaaggcgc
    15001 tacagactta tcttggcagt acagtgatta ttatctccca acgtgtccgc tctattcaag
    15061 aagcacatca tatcttagtg atggatcaag gtaagattgc tgctcagggt acccatgaag
    15121 aattacttgc tcaatcagca gaatatcagg aaatcgtcgc atcacaggag gaagaatagt
    15181 tatgaaagta aaaaaattat ccttgcagac gctccgccga ttcattcctt accttactgc
    15241 ctatgcaaaa gacatttggc tagctgtgat actaggaatc atcaatggga ttgcaacagt
    15301 tgtgatgacg ttacaaattg gtcaaagtat cgatcaaatg attggagaag gacaggtccg
    15361 cttcaatcga ctgttccatt tacttattct attcggtggt atcgttttaa tcaacgtagt
    15421 cagtcaatgg ctcatccaag tgttaagcaa tcggttagct tacttatccg ttgcaaaatt
    15481 aagaaaagat gcatttgctc atctgaatcg attgccactt cgttattatg atcaaaactc
    15541 ccacggaaat atcgtcagtc gatttacaaa tgatatggac aatatttcca ttgcaatttc
    15601 tgcggccttc aatcaattat tttctggagt ggcaatcatc attctttcgt ttatttttat
    15661 gatgcgtcta agtccacttc tgacattggt cgttctttgt tctacaccaa ttattttcct
    15721 tgtgagttgg atcgttgctc gtgcttcaca aacaaatttc gatagacagc aaaggattat
    15781 tggggatatc tcgggctttg tgaccgaacg aatcggtaac cagaaaatcg tgaaagcttt
    15841 tcagcaagaa gaagctagtc aacagcaatt tgaactgcta aatgctgatc tatatgaaaa
    15901 aggacaacgg gcacaatttt cttcttcctt gacaaacccg tcttctcgtt ttgtagatca
    15961 ccttgcttat ttatcgattg gtttagttgg cggcctattg gcattgaaga gtggttcgtc
    16021 aatcactgtt ggtgtgattt ccagctttac gatctactca agtcagtttt ctaaaccatt
    16081 tatcgaactt tctggaatca cgacacaact gcaaaccgct ttttctggtc tagaacgagc
    16141 atttgatttg atcgatcagc cagaagaaaa gcctgatttg ccagatgcct atgccttgga
    16201 acccaaaaat atcaaaggaa aagtctcttt tgatcatgtt tctttttctt atgaccccaa
    16261 gcagcgtttg atcgaagatt tcaattttac tgcttatcca ggtcaaacca ttgctatagt
    16321 aggaaaaaca ggggccggaa aatccacact tgttaatttg ctgatgcgtt tttatgacac
    16381 aacaaaagga acaattacag tcgatgacca tgacatccgc tctatccaac gtgatacatt
    16441 acgtaaaagt tttggaatgg tgttacaaga tacttggcta tttgacagta cattgcgaga
    16501 aaatcttcgt tttgggaatc cggaagcaag tgacgcagaa atcaatgatg ccctaaaagc
    16561 ctcttatatg tatgaatttg tagaacgttt gccggaaaaa ttagatacta agattggcgg
    16621 tcaagggtta aagatatcag atggacaacg ccaactgttg acgattgctc gaacgatgat
    16681 cagtaatccc cctatgttga tcttagatga agctaccagc tcagtcgata cattgacaga
    16741 gaaaaaaatc caacaagcct tcttagcaat gatggaagga aaaaccagtt ttgtgattgc
    16801 ccatcgcctg tccacaatca aaaatgcgga ccagatatta gtgatggatc agggagctat
    16861 tgtcgaaatg ggcacccatg atgagctgct tcaaaaagat ggttattacc gacggctcta
    16921 tcaagcacaa ttcacagcac aataaaaaca agcagtcttc tgggatggga agactgcttg
    16981 ttttttattt agcgtggatc gctaaacttc ttaaaatttt
//
LOCUS       DADVXN010000061        16968 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.063, whole genome shotgun sequence.
ACCESSION   DADVXN010000061 DADVXN010000000
VERSION     DADVXN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.063"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(26..2110)
                     /gene="cadA"
                     /locus_tag="KB942_001738"
     CDS             complement(26..2110)
                     /gene="cadA"
                     /locus_tag="KB942_001738"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6852160.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2635..2994)
                     /locus_tag="KB942_001739"
     CDS             complement(2635..2994)
                     /locus_tag="KB942_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBB6852161.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3024..3365)
                     /locus_tag="KB942_001740"
     CDS             complement(3024..3365)
                     /locus_tag="KB942_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852162.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3362..4036)
                     /locus_tag="KB942_001741"
     CDS             complement(3362..4036)
                     /locus_tag="KB942_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6852163.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5212..6510)
                     /gene="asnS"
                     /locus_tag="KB942_001742"
     CDS             complement(5212..6510)
                     /gene="asnS"
                     /locus_tag="KB942_001742"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBB6852164.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6550..7740)
                     /locus_tag="KB942_001743"
     CDS             complement(6550..7740)
                     /locus_tag="KB942_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6852165.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7761..8288)
                     /locus_tag="KB942_001744"
     CDS             complement(7761..8288)
                     /locus_tag="KB942_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBB6852166.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8335..11124)
                     /locus_tag="KB942_001745"
     CDS             complement(8335..11124)
                     /locus_tag="KB942_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6852167.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11271..11471)
                     /locus_tag="KB942_001746"
     CDS             complement(11271..11471)
                     /locus_tag="KB942_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6852168.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11924..12244)
                     /locus_tag="KB942_001747"
     CDS             complement(11924..12244)
                     /locus_tag="KB942_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852169.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12747..13865)
                     /locus_tag="KB942_001748"
     CDS             complement(12747..13865)
                     /locus_tag="KB942_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852170.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14336..15643
                     /locus_tag="KB942_001749"
     CDS             14336..15643
                     /locus_tag="KB942_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBB6852171.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15764..16963
                     /locus_tag="KB942_001750"
     CDS             15764..16963
                     /locus_tag="KB942_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6852172.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT         4803 a         3606 c         2980 g         5579 t
ORIGIN      
        1 aaaggatata aaaaaaagag gacgttcatt cgtcctcttt ttttacatgc aagcttcgca
       61 tggcattcaa aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg
      121 atgcaaaacc aaaagcacct aaggcaagta ccaaaatttt tatcccaata gcaaagacaa
      181 tgttttgttt gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg
      241 atggttgatc attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc
      301 cacccattgc gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc
      361 cgacaaatgc ggtcttttta ttgtttcttt gtaagatttc ctctaaccgc tgcactttat
      421 cttcaggaag caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag
      481 caattttttt cgagtcgccc gtcaacatga ctgtgttctt cacaccagcc tgttttaatt
      541 gaaccaatgc gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt
      601 aactttgatc gatcgctaga taaagcaatg taccgatttc ttgacttaaa ggaaaagaga
      661 tttcaaatct ttccatcaat ttgtgattac ctgctaatac ttgtttgcca tcaacttcag
      721 ctaagatccc ttcacctgca aattcctgga tattagttgt tggtaaaagc ggacctgcgt
      781 agccttctaa aacagattga gcaatcggat gtgtagaaaa ctgttctgca ctcgcaatat
      841 attgcatgaa tttctttgga tcgatttttg tatcgatcgt ctggatcgaa aagtcacctt
      901 tcgttaatgt gccggtttta tcgaatacca ctgtctcggt ttgcgcaagc aactccaagt
      961 aatttgaacc ttttactaat acaccgattt tacttgcgcc tccgataccg ccaaagaaac
     1021 taagtggcac ggaaatcact aatgcacatg gacatgagat cactaaaaat gtcaatgctc
     1081 gatagatcca ttcagaaaac gccgctccag aaacaacaag tggaggtaaa acagctaata
     1141 aaacggccaa tcctacaacg actggtgtgt aatagcgcgc gaatttagta atgaaatttt
     1201 cagctggtgc ttttttactg ctggcatttt ctactaattc taataatttg ctgatagtag
     1261 aattttcata agatttttct acttgaacta aaaatggttt atttttattg ataaatccgc
     1321 ttaatactgt ttcaccaaca tatacagatt tagggactga ttctcctgta agtgctgacg
     1381 tatcgactaa tgattctcct tcgatgatcg ttccatctaa cggtacacgt tcaccaggct
     1441 tcacaagtac aaactgaccg atctgcacgt cttcagggtt cacggtcatc aatccttccg
     1501 tcgtctgcac gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc gattttcttg
     1561 aatgactgac tgccaatcct tggaaatact cgccgatttg ataaaatagc atcactgcta
     1621 cggcttctgg atattcaccg atcaacatag cgccaatcgt agcaacagac ataagaaaat
     1681 tctcatcaaa gatttttcct ttgacgatat tcatcactgc cttcttcact acatcatatc
     1741 ctatcagcag ataaatcaat aaatacccta tcaagcgaat tggttcatta aaatcaaata
     1801 atagcaataa agcgatacct gcaagggaaa ataaaatccg gaacagcgta tttctctctt
     1861 tttcatttcc atgttcagat actttttcct tatctatttc tgttactttg acatctggtt
     1921 ctaattcctt cactactttt tttgtttctt gttcaatcgt agccaaatga tcttcctctg
     1981 cgtcaatcgt tagttttcca gaagtgaagt ttaccgttgc ttccttgact ccattgatct
     2041 tctgaactcc tttttcgatt ttcattgcac agttcgcaca atctaaacct tctaatctgt
     2101 aacttttcat tttaatgacc tcctcgcctc aacatatgaa catctcttca taactaatta
     2161 taaatgaatg gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat
     2221 cggaaaattt cttccataca gatgttttgg cttattcttt ctttttctct tcgatcattg
     2281 cccctgctct tatccaaccc gcacttgcct tcttgatctt gacaggatag caactaagtt
     2341 tgaagcctgt tgctggcaat ttgtctaaat tcattaattt ttcgatagga caatatcttt
     2401 tttcaatccc tgcaaaatgt ccttcccaga tattcgaagc tccactacgc tttcaaaaat
     2461 atagaaagag ttctttaatg aaaattattt caactttcca taaacagtaa tcccatataa
     2521 ataaaagacg taaaagtcac atgggagaac ttatacagga aaacacatag agtcaggtga
     2581 aatacatgta tcccacctga ctctatgcgt tttctaacct ttattattct ctttttatac
     2641 aatttctgca ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa
     2701 aggtatccct atgatcgtga tacatagaag gatgccgctg acgacatgtg cgagagcaat
     2761 tggtaggcct gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg
     2821 ataaattact tctttgccaa aaggcgcaaa agaaagggag gcgatcttaa aacattgcat
     2881 accgattggg atacctatga tcgtgataca ccaaagtatc cccgctgcta accagctaag
     2941 accaccgatt agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt
     3001 tctccttcta ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt
     3061 ttagcttcca gcatcaagta ttctgcaaat tttttttgga tttcttccgt attttctttt
     3121 gttactaatg tgtgttggat tagttttcct gatggtcgct gatccacccg ttcttctgac
     3181 tgttgcacgt ccgcttgcgc aaatccgata atccgttttt cttttgttac agcaacgagc
     3241 aatagtttat tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga
     3301 tgatcagtaa tcagctgatt gatttgaggt aaatcttctt ttactgccca ccgtatttcc
     3361 atcatttctc acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcggcaa
     3421 ttgcatctat ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat
     3481 cttctcttgt atatttcgaa aaaccataag caatccctaa agcattgaat atgccatcta
     3541 aaggaaccag caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt
     3601 ctaagtcttt acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca
     3661 agatgatgtt gcagatttcc gctgtttgaa gttcatttag taattttcga taaacggatt
     3721 caaattgctg cgcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg
     3781 tatcattgat tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctt
     3841 gccagcgtac ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa
     3901 atttcatcgt tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac
     3961 ctagatcagt tgcttctgct cggtctcttc ctgcgtctgt aatgctgtca ccgataaata
     4021 atatacgttt tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt
     4081 atattatccc ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc
     4141 agcctagtcc cttcaatcaa ttgaatgaac gctcatcaag tgtctagttt tttatacttt
     4201 ttttcttaac gtgccggatt ccccttgatt ccttcgttgt ttggtccaga gaaatcgatc
     4261 acacctaagc ttccgtcaat cagaccactg atgacgccat cagaacatat atttataggc
     4321 tcatctaaga cttccagtaa atgttctttt actaatatct cttctagatt gcttgggata
     4381 gattggtcat aactttatct ggtccgatga tttcaaggat gaattaaact tacatatgga
     4441 tcattgcatg ctgaaaatac ggacctactc catcttcgaa tgttactatt agtttgtttt
     4501 caggtactag atgctgcttt atttcaacag cagaagcatc atcaaatcag atttccattt
     4561 gaataacctt ctttgtatat gtttgattga ttgcgatttc gttatagaac cttgtgaatc
     4621 aaatagcaat ttcttttgct ttcttctctt gtctgctgga aacttccttt atcccgtatt
     4681 ttaaaaaaag aactgcgaga gttcgcagtt ctttttttta aataaacgtt gctctccggc
     4741 caactcctcg gtcacaagtc acaatagtca tcaaacatga aaaactgttt tttgaaatta
     4801 ctccttgtga ctcggagctg aacggcaggc gcacaacctc ttgatataaa cggtgttcaa
     4861 tcagctgacc ctcgatatta gctaaaaaaa cggtaaaata tgagaagcta tttttccgtt
     4921 ttgtttctct aatactcagg tcaaggcact gatttcacaa cctcttcctt aaacggtgtt
     4981 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa gccatttcca
     5041 caaaattttt cttaatactc gaaactaaac agctttttca caacctctcc actaaacggt
     5101 attcaaaagc ttacatctgc ggtaataagt cccgccaatt caaaaacatg aacagctgtt
     5161 tttgcttggc tgttctaatt acttgatgca gaacagcttt ttcatcacct cttatggata
     5221 aattcggttc aacaaacgtg ggaatggaat agcttcacgt acatggtcga taccagcaat
     5281 ccaagtgact gttcgttcaa ggcctagacc aaaaccagag tgcggaacag aaccgtattt
     5341 acgtagatca agataccatg aatattcttt ttcatccaaa ccgtgattac ggatttgttc
     5401 tagcaagaaa tcatagtctg ttgcccgttc gcttccgcca ataatttcac cgtaaccttc
     5461 tggtgcgatc atatctgcac aaattaccac gtcttctctt gttggatgtg gtttcatgta
     5521 gaatggcttg atggcttttg gatagtttag gataaagaca ggctgttcga atgaattcgc
     5581 aatgaacgtt tcatgaggtg aaccaaagtc ttcgccccat tcgatgtcat caaatccatt
     5641 tttcttcagt aattcgattg catcatcgta agtaatacga gggaatggca attttgtata
     5701 agtttttaag acttctttgt ctcgtcctag tacatctaat gcatattcgc aatgatctaa
     5761 aacattttgt actaagaatg ctacatattg ttcctgtact tctaaacttt cttcttgatg
     5821 agtgaaagcc atttctggtt cgatcatcca gaattcgatc aaatgacgtc gtgtttttga
     5881 tttttcagca cggaaagtcg gaccgaatga aaagactttt ccgaaagcca tagcagcagc
     5941 ttccatgtat aattggccac tttgagataa ataagctttt tgatcaaagt aatttgtttc
     6001 gaatagatcc gtagtgcctt ctggtgcaga accagtcaag atcggcggat cgattttgac
     6061 aaaatgatgt tgattaaaga attcatacgt tgcacgaatg atttcatttc tgatctgcat
     6121 aatcgcatgt tgtcttgaag aacgcagcca taaatgacga tgatccatca agaaatctgt
     6181 tccgtgttct tttggtgtga tcggataatc gtggctctcg cttaccactt ccacatttgt
     6241 aattccgatt tcataaccaa attttgaacg actgtcttca cggatttctc cagttactaa
     6301 cacggatgtt tcttggtgta gatttttagc tgtttggaaa acttcttccg acacttcgct
     6361 ttttacaact acaccttgga aaaaggctgt tccgtctctt aattgtaaaa atgcgatttt
     6421 tccgcttgat cgcttatttg cgacccaagc gccgattgtt actgtttctc ccacatggtt
     6481 tttcgaatcg atgatttgaa tttgttccac gtatgactct ccttttatat tccttgaatt
     6541 tcttcaggat taattttgac ttttactctc aataaattgc ttgattctgc gtaccgcttc
     6601 ttctaacgta gcgcgatcag tggcataact catgcggaca ttttctggtg ccccaaagcc
     6661 ttcgccagtc acaagcgcta cccctgtttc ttctaaaaga tcttctaccc attgtgtgac
     6721 attttcatac ttacacatgc gtagcgtttc ttttacatta ggaaaaagat agaaagctcc
     6781 ttgcggcttt tcaagagaaa cacccggcag ttctgccaca agcggatata acgtattcag
     6841 gcgttcttca aaggcgttcc gcatttcttc tactgtatct tgctcacctt tcaatgcttc
     6901 aacagctgca tattgactga ctgctgtcgg attgctagtt gattgggaag caacagcgat
     6961 cattccattg atgatcacag gatttcccac cgcataacca atgcgccagc ctgtcatcgc
     7021 atatgtttta gacacgccgt tgatgatgat ggtctgattt ttgattgctt ctgaaatcgt
     7081 tgcaattgga gtaaactcat tgccattata aactaaacgg ccataaatat catcagcaac
     7141 tatcaaaata tcatttttta ctgcccattc accgattgcc tgcagttcat tcttgctata
     7201 aatcattcca gtcggattcg atggagaatt gatgattacg gcttttgttt tttctgaacg
     7261 tgcttgttct agctgttcaa cagtaacttt ccagctattg gattcttccc ctttcacaaa
     7321 gacaggaagt ccttctgcca gttttacttg ctcaccgtaa ctgacccagt aaggcacagg
     7381 aatgatgact tcatcttgcg ggtcaaggat cgtttggaaa agggtataca aagcaaattt
     7441 cgctccgtct gttacgactg tttgtgaagg ttcatagaca agtccatagt ttttcttcag
     7501 ataatcaacg attgcctgtc gaagctctgg gattcctgct gttggtgtat aatagctagc
     7561 tttcccattg cggattgctt cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc
     7621 cggttctccc acagtcaaac tcagcacatc tctgcctttt gcttttaatg ccttggcctt
     7681 tgccgcagct gccaaagtga cagacggttc taatcgtttt gttctttttg ataactccat
     7741 cccattcatt cctttacctt ttaaattccc ttgattgtct tgacttcatc tccagattca
     7801 aagccaatca ggtaataatt cagtttccca tttgtttctt ttcctactac ttcccacaca
     7861 ggctgatcat catacatccc taaagcagct ttttcgattt ggatttcagg atgggccgca
     7921 gcaattgtct gttttgcttc gttttcagtc agtccctttg tttgatccaa tacttttact
     7981 ttttcaccag atttaggaat gataacggcg atttcttttc ctttatcatt tgtacctgtc
     8041 agtgaaaagt atgtcttctt tcgagtaaac caataaaaat gatcggtttc ctcaagattt
     8101 gcatatttct ttgcgatttc tactgcttct ttcttcgcct gcaccgccgg acgactggaa
     8161 cgcaagtaaa aaatcgagct gatcacgatg accgctaata aaaatacgat caagccaagt
     8221 aacacgcgat taatcgtttt ttcctttgtc tgttttttct tcaagggtcg tcgctccttt
     8281 atcttcattc cttttcatta tagcagattt taattatgaa aaaagacagc tcctctaact
     8341 tccttcttca tttttcttta agaattcatg gatatcctga tagatttcct ccatagctag
     8401 ctctttgatc ggtacttttt tcggtaatgc atggatgatc cgtgcgccat attttgcagt
     8461 gactagtctt cgatccaaaa taagcaatac gccacgatct gtctccgtcc ttatcaagcg
     8521 acctaatgct tggcgtaact tcaaagctgc tttaggtacg gattcctggt aaaaaggatt
     8581 gatcccctct gattctaaat aagcgttcct agctttgacc atcgggcgaa gcggattatc
     8641 gaagggtaaa cgtgtgacta caacgatctg caaagcatct ccaggcaaat ctacaccttc
     8701 ccaaaagctg tcagcgccaa acaatacgct tttttctgac aaaatgaagc gtttcaaaag
     8761 cttctcccgg cttccaccaa acccttgagc tagtatttct cgtccttttt ctagcataga
     8821 cagatgcatt ttctgataga ccttctgcaa aacatcatga gaagtgaata acacaagtat
     8881 tggccgatct tcttgttcga tcagacctgt aagtgctgtc tctaaataat cgatatatgc
     8941 ttgattgctg acttgatgga cggcaggtgc ctcttttggc acatagatcc ttgcctgttt
     9001 ctcataatca taaggagagg caatcacttt aagaggtgct gcttcaatgc caagcctctt
     9061 agagagatag tgtcgattag ggccgatctt cagcgttcct ccaatataaa gaatacgatc
     9121 atatcgagga taccaaacag tcgacggcaa catagccgct tgaaaatcat ggagatgaaa
     9181 aatcatctgc tgcgctgtgt tcccaggaac gaaccaatgg atcaaatgat ctcgccagtc
     9241 ttccagccat tgatccatca gcaagtattg cttgtctaat tgatcaaata ggctatataa
     9301 acttgctaaa tcgatccgca tctggtttgt ccagctttct tggataatct caaaagtcag
     9361 acgcatttgt gactgtagct gctggatctc ctgataaaaa agctgcaggt gatcaatggc
     9421 tgtcccagat tccagtggca gctgatccaa catctctttt gtcatcatga cttcttctgg
     9481 atactgtccg ttaggttgct tgccgctcac taaactcatt aatccttctg ccatacaaat
     9541 ctgtgcttcg catagcgcag aaagttcttc tcgataaatc gataaccagc gtaaggcttc
     9601 atgttctttc ggtaaatagc tttgtattcg gtcaaacagc tgatttggtt cttctaattg
     9661 ctggatttgt ttatggaaat agttaatact taatcgtttg tcatttactt tttcagcgat
     9721 ttccggcaaa tgatgtactt catcgatcaa caaaaatgga ctagctggaa gcaatggttc
     9781 ttttcgctgt gtttcttgta ctaaaaacgc atgattgaca atcaaaaaat tactttgcct
     9841 gattttcttt tgcaagaaac gaacaaagtc ttgttcgtaa aatgactgat ttcttgctaa
     9901 aaatccaata cctctgtgcg cgatatctgc aaagaatgaa tggttcaaat ttgttaaatg
     9961 taattcatcc aaatcaccag tcttcgtttg cgtcagccaa actaagaccg ccatttgata
    10021 caaagcatat tgtttttgtt ctggcggttc cacaagtgtg cctttaaaac gctgcaaatc
    10081 aatatagtgg cgatagcttt tgataattac aggttgtatg ggctgatcta gcaattgatt
    10141 cagcaatgga atgtcttttt ctatgatctg ctgctgcaaa actagcgaaa cggtactgat
    10201 gacagcgggt ttttcaggtg tagccaaata actcaatggc agcagataac cgattgtttt
    10261 acccattcct gtagctgctt cgatcatcaa atcttttgat tcatcttttg tgaaatggtc
    10321 gtagaccgca ttcatcagtc tgttctgttc ttttcgatac atcagtgttt taccaaacat
    10381 tttttctttt gcttgttttt ttcttggata tgtccgctcg ccataatgga cggatgtgaa
    10441 tagttccact tcttttttct gcaaagctag cccatcgacg atttctaatg agggatcaag
    10501 tggctctggt ttttctttca tttcttcaat cacatgataa atgaaacgac tcgtatccat
    10561 acctgtgtgt ctacttaatc gcgcaatctg ctctaacgtg atgattggca attttctcat
    10621 ccgttcttcg atcagcaaaa gaagttgacc tgtcacttcg gcatcactat ctgcctgatg
    10681 aggattctca tgaataaatc caaggctttc tgacaaatca gctaagcgaa agcttggttc
    10741 cgtcggcaag aaaatttgtg caagttctac tgtatcgatg cctggtatct tcaaactagg
    10801 aagtccgcat cgcatgagtt catggttcaa aaaattataa tcaaaataaa tattatgcgc
    10861 gacaaatatg gtgtctgcca aaagattgta aatcgtttgg gcgacatctt cgaaataagg
    10921 cgctttttgg acgcgttgat tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt
    10981 ccgccctgga tgaatatcga tcgaaaaacg tgaagtgatc cgacctgatt caatcatgac
    11041 gcagccaaat tggatgatcc gatcttcttt tggattcgtc ccagtcgttt cgatatcaac
    11101 gactgcataa gttcttggtt tcaaaatatc ttcctcttct cctgcctcta atgttctttt
    11161 gaaattatac tacaaagtgc agaaaaatac tctcatcgct ttttaaagaa aatagacaaa
    11221 aacgcccaga cacgcaaatg tctgggcgtt tattttttag tataggttaa ttaagcttta
    11281 gtaacgtttg ttgcttgagg tccgcgttgg ccttcttcaa catcgaaagt tactgcttgg
    11341 ccttcttcta aagttttgaa tccgtcgcct tggatagctg agaaatgaac gaatacatcg
    11401 tttccgtttt ctgcagtgat gaatccaaat cctttttctg agttaaacca ttttactgta
    11461 cctgtttcca taataaaaat cctccttgtg tatcaacaca tattttgcaa ttacttgaat
    11521 ggtgtatatc tggaagatgt ttatttgaaa caagctacca cagattaccg aacaaaaact
    11581 actcctatag tatatcatac cgtttacaaa aaagctagag tttttcgact ttttttatta
    11641 aaaaaagttt tctcagcgat tacataaagt gaaaaaccga actttttttt aaaataaacg
    11701 acatattgtt cagttggtgt ttttttgctc tgatgttctt ttttaaacta aaaaaattat
    11761 tctttctgac tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta
    11821 ggtctgatgt tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa
    11881 accaatacga ccaacgagat tagtttttct tttttgctca tatctatacc tcataaataa
    11941 caggatgatg tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat
    12001 gtaagaaatg ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc
    12061 ttggctgcaa agaccgttct tctgtttcag gaatcgcaga atcaagatct ggtgtctgta
    12121 cataaacatt tttgtacaga cgtgtgcctg taataggaaa ccgctgtgtt tcttcagcaa
    12181 aaactggctg acttgttaaa cttagggata gagacagcaa acaccataac agtctttttt
    12241 tcataaagac ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc
    12301 gttattcaca ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg
    12361 tgaccacgtc acaacctctt ttttaaacgg tgttccagaa gtagcttctt cggaaataag
    12421 ccgaaatttc acaaaaattt gagaaataat ttttgtcaat ctcttcttat ttcttgaagc
    12481 tgaccacttc tgtcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    12541 taagcccacc aaacaaaaaa tatgaagagc tatttttgct tgctgttctt attacttgat
    12601 gcagaacagc tttttcacaa cctctcatac acggtgttta atcagctgac cttcggtatt
    12661 agctcaaaaa acggcaaaat atgtgaaact atttttccgt ttgtttctct aatactcagg
    12721 tcaaaacact gatttcacaa cctcttttaa taacgaagaa tcgctgtcag actcttttca
    12781 tctggcgcat ctttttgatc caaaataaac acttttccgc cattaatgat cacatcatct
    12841 gctaatgcat tcaacacttg acgccgatca tattctgttt caggatcttc accgaaacca
    12901 tcagctaagt ttgacgtagc gataaataaa tgtgacactt ttccttcttt cgcagctgga
    12961 acgatatcaa ctaactgatc gatgaatttc ttatctaaga gtttattgta ttcttttgat
    13021 tcttgagctg cgagttcact tgacagcttc ttcgcccctt cttcgatatc tctaaaggat
    13081 agctgcgcag gagaagcaga aatcgcaacg tctgcacaat aatagggatt tttagcaatt
    13141 tttttgaaca tcgtctgatt ttctggtaga gcaaataaat aaatcggcca ttgttctgga
    13201 ttttcgaaag aatccttcaa gtacgtatca actgcttggt aatagttgac ccaatcgatc
    13261 tctacttctt catcttttgg atttacgcca tggaaagtta ctccatctcg ggctccgcct
    13321 cctccattag aagagaagtt caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg
    13381 acatcaaccg gtgccccttc tggtagagat acttctttta cttgtttgtt gctgacttca
    13441 tacaatttca tggagtcgcg gttcaatccc aataaataat aagaatagtt gaactgccca
    13501 tttttgataa tagccagtaa ataaggaaga tcacttacat agtattgatc gtctaccggg
    13561 atactcaaac gatggatata ggtttggtca gcagttataa tgaccgctac actcgtagtt
    13621 gcactacgcc aaaacgaagg atctcctaac aaattttcaa ttttttcttg gaacggggac
    13681 cagtcatgtt cggaatattt tttctcaaaa cgtttctttg cttctttcgc aaaatttttg
    13741 aatttgatct gatctttttc cacatcttga tgggcgacat gcgtattcag catgatcgtg
    13801 acgaatggtc ctttagcgcc ttctgaagtc aattgtgaca ataattcttt atcctttttt
    13861 gccataaacg tacccctcct aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca
    13921 acactatttt tccatacaat aaaacgtatc taaaaaaata aaacggtttc tacggtttta
    13981 aatttagcta tttttttaag ttatcttcat aatttcctca attatttagc cccagtcttc
    14041 aactattctt catgaaatgc tgataaacta agaaaaagaa taaaagaggt ggatcttatg
    14101 catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt cagcgcttcc
    14161 cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa
    14221 ccttccataa cgtgcgttct tatcctaaag ttttttcaga aacattgaaa ctctctcccc
    14281 tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat cggatatgtc
    14341 cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt
    14401 tctaccagct gttgctgtgc aagctgaaga tactttcaaa gtaaatgcaa aagcagcctt
    14461 tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg
    14521 gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg
    14581 gaaactatca tgggatgata aagtttcaat ttcagattat gcagaaaatt tgagtgtcac
    14641 cccagattta tcaaatgttc ctttgcataa agaaaacacc tacaccgtta aagaattatt
    14701 cgactctgca atcatccagt cagccaacgc ttcaatggtt gccttggcag aaaaaatctc
    14761 tggcagcgaa gcaaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggtatcaa
    14821 agatgcaacg atcgttaatg catcaggact gaataactct tatcttggcg aaaaccgacc
    14881 agaaggtact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ctatcgttgc
    14941 ccgtcatttg atcttggatt ttccagaaat actggatgta tctagcacta cgacacaaat
    15001 gtttggcgaa aacacgcaat caccagtaga aatggtcaac tggaactgga tgctcccagg
    15061 attcgtgaat tataaagaag gcgtggacgg attaaaaact ggtacgactg aacttgcagg
    15121 agcctgtttc gtcggtacga tcactaaaaa cgatcaacga atcatcactg tcgtattgaa
    15181 tgcagacggc catgcggaaa acccaagtgc ccgtttcaat gaaacagctc gcttaatgga
    15241 ttattgctac gacaattggt cagaaaaaga gcttggaaaa gccaatgcaa gtatccctag
    15301 tctaaaaacc attgatgtaa aagacggaaa agaatcaaca gtgcccgttg tgcttaaaag
    15361 tcctgtaaaa gtatgggtac ggaacgatat ggatactgga aaattaacaa tcacaccaac
    15421 tcttgacaaa aaacaagtca ctgacaatga gatccaagca cctatggata aaggaacaaa
    15481 aatcggtacc gcaacgatca ccttggcaga agataaatta ggttatcttg aagacaacaa
    15541 cgcgccatcc acagaaatca tcacggataa aaccgtagaa aaagcaaata ttttcgttat
    15601 tggctggcga cgcgtcgcag aatttttcgg taacttgttt taattgaaca aaaagaaaaa
    15661 agcatctttc catgtggaag atgttttttt gctttttcta gtttaaataa cagctttttt
    15721 catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc taaccagaga
    15781 tctgattttt ttggttctct atcttattgg ggtattctcc atcgtttatt ggcgaaaaca
    15841 gaaaaaggat ccttccttgt tcttttacag cagtacatgg acgtttgcgg taatcttgac
    15901 ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc ccttagtgtt
    15961 tttcagtatc tttgcctttt cctatttttc agaaaagcga aggctgttga atggtctttt
    16021 attcaatatt ttcctcatta gttttggtat ctacttgttt gtcttgctat acgaaacaca
    16081 aaatattttt cttggcggac tgattgcctt gatcacgatc ccgttactgc ttgtctttct
    16141 attcggcatt tatggattga tcgtttttct tttttggaac ggtgtcacag ttttacgtag
    16201 agagtcgcat tcattagcta atcttttaac gttgatacta gccatttttc ttacattgtt
    16261 tcttgttttt gactttttct tgttaaaata tttaccacaa tggataaatg ctttgttttt
    16321 ctgcatccca ttgatcttga tctatctatt tatcgtattc tacaactttt tgactgtctc
    16381 attcttgtat cagttcaacc gccctcgata caaccaagac ttcatcgttg tgttaggcgc
    16441 cgggttgatc aatggagaaa cagtttctcc tcttcttgcc aagcgaatca ataaggcgat
    16501 tgctttttat tgggcacaaa gccgtgcgac attaaatccg cctatcctat taatgtcggg
    16561 tggacaggga gcagacgaaa aagtaccaga agcaatggcg atgaaacaat atgccatgga
    16621 acaaggaatt cctgaaagag atattcttgt cgaaaccaat tcgacaacca ctttggagaa
    16681 tatgcgctat tcaaaggaaa tcatggatca gcaaatgaaa ggtccctatc gcgcgatctt
    16741 cagttccaac aattaccaca ttttcagagc tggtctctat gcacgacaag cgaaactgaa
    16801 ggcaaatggc attggggcaa aaacagcttt ctattacttg cctaacgcgt ttttaagaga
    16861 atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg gattgctttt
    16921 cgcaggttcc gcattcttat cactgatcac tttacttttc taaaaaaa
//
LOCUS       DADVXN010000062        16944 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.064, whole genome shotgun sequence.
ACCESSION   DADVXN010000062 DADVXN010000000
VERSION     DADVXN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.064"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            279..1064
                     /locus_tag="KB942_001751"
     CDS             279..1064
                     /locus_tag="KB942_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6852173.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1077..1928
                     /locus_tag="KB942_001752"
     CDS             1077..1928
                     /locus_tag="KB942_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBB6852174.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2052..2504)
                     /locus_tag="KB942_001753"
     CDS             complement(2052..2504)
                     /locus_tag="KB942_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852175.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2969..3337)
                     /locus_tag="KB942_001754"
     CDS             complement(2969..3337)
                     /locus_tag="KB942_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBB6852176.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3487..3969
                     /locus_tag="KB942_001755"
     CDS             3487..3969
                     /locus_tag="KB942_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBB6852177.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4006..5016
                     /locus_tag="KB942_001756"
     CDS             4006..5016
                     /locus_tag="KB942_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBB6852178.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5131..5844)
                     /locus_tag="KB942_001757"
     CDS             complement(5131..5844)
                     /locus_tag="KB942_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852179.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6034..6624)
                     /locus_tag="KB942_001758"
     CDS             complement(6034..6624)
                     /locus_tag="KB942_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBB6852180.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7108..7335
                     /locus_tag="KB942_001759"
     CDS             7108..7335
                     /locus_tag="KB942_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBB6852181.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7351..8517
                     /locus_tag="KB942_001760"
     CDS             7351..8517
                     /locus_tag="KB942_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBB6852182.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8751..9794
                     /gene="hrcA"
                     /locus_tag="KB942_001761"
     CDS             8751..9794
                     /gene="hrcA"
                     /locus_tag="KB942_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBB6852183.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9888..10451
                     /gene="grpE"
                     /locus_tag="KB942_001762"
     CDS             9888..10451
                     /gene="grpE"
                     /locus_tag="KB942_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBB6852184.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10502..12331
                     /gene="dnaK"
                     /locus_tag="KB942_001763"
     CDS             10502..12331
                     /gene="dnaK"
                     /locus_tag="KB942_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBB6852185.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12482..13648
                     /gene="dnaJ"
                     /locus_tag="KB942_001764"
     CDS             12482..13648
                     /gene="dnaJ"
                     /locus_tag="KB942_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBB6852186.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13730..14395)
                     /locus_tag="KB942_001765"
     CDS             complement(13730..14395)
                     /locus_tag="KB942_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852187.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            14371..14544
                     /locus_tag="KB942_001766"
     CDS             14371..14544
                     /locus_tag="KB942_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852188.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(14589..15326)
                     /locus_tag="KB942_001767"
     CDS             complement(14589..15326)
                     /locus_tag="KB942_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6852189.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(15328..16830)
                     /locus_tag="KB942_001768"
     CDS             complement(15328..16830)
                     /locus_tag="KB942_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBB6852190.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT         5552 a         2937 c         3488 g         4967 t
ORIGIN      
        1 aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga
       61 agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc
      121 tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca
      181 tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc
      241 gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca
      301 aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg
      361 ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag
      421 cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt
      481 ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg
      541 tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag
      601 attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt
      661 taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg
      721 aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat
      781 ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc
      841 ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac
      901 tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag
      961 ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg
     1021 gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg
     1081 gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa
     1141 agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg
     1201 gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta
     1261 gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact
     1321 tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt
     1381 tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag
     1441 cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa
     1501 aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg
     1561 tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa
     1621 tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt
     1681 ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg
     1741 cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa
     1801 acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag
     1861 aaaaaaaatt aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct
     1921 ttatttgagg ataaagatgg taaaatttca tttattaaaa aaatatttgt ttgaaccaaa
     1981 cagtaagtac ttataaaaaa atagcgctag aatttccaac atctctggaa tttctagcgc
     2041 tttttaattg tttaagatac aagggaaata tctgtctgga tcaataattt ttcattgatc
     2101 cattgactaa agtttacgac ataattgaag ttaccaccga ttagtatctc cgcatgattg
     2161 tctttataaa gatcgtattg taaaagacgg tcttttttat ttgcgtaaac atcgttatgg
     2221 tactggacta attgttctcg gaattgattg attgctgtaa aatcacgata ttgaaggttg
     2281 aaacgcatcg tgattggtgc atttagcatg aatcgctgca tttcttttgc tgcttcctct
     2341 gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca ttgcccgcca
     2401 acctttgttc cttttagttt tccttcatga agataatttc tgatagaacg tgtagtaaca
     2461 cctaattgtt cggctaattc atttactgag taaaaatctt tcaatgttca ccctcctatg
     2521 tgaatggttc tactgactta gttgatattt gcttaatcac gaaatgtaga aagaacgatt
     2581 ttaattaagg tcattgtaaa aatcaagtca gaaaattcta acttttattt ctctaaagaa
     2641 attcgttctc tcctaaagtg atattatatt taaatatacc tgaaaataat gggcagtatt
     2701 atgaaagatc actgatattt tctcgtattt tttccttatt caaaagtttt gcaggaaaat
     2761 tgctgaaaaa ataattttac taagtagaaa attcaaagcc attcaatcag tgattttttt
     2821 cagcacaaga aagactcctt ataaaaatag cagatacttt gtcaattaag tgtttaacca
     2881 gggatgataa aaaggatagg attgaacaaa acctgtataa ggtgatgaat agtttcctcg
     2941 ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa tttatcatga ccttttttat
     3001 cttcaatatt aaatgaaatc aattgttcaa tcaattccca gttgattaca cttttccaag
     3061 gaactttgat gacgttatcc gtatatacgt agcctgcttc ttggatattt ttttcaaggg
     3121 cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa ctaaatgcaa
     3181 taataaaggt accatggtca gtaaacatag gttggttcca ttttacagtt gtgtctagct
     3241 ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat cttttgttga tgttcttttt
     3301 ctttgatttg attgatgatc ggctgaaatt tttccattct aaaacgcctc cattaagtgg
     3361 tttgatttat tttaccagaa aaacaatagt tatgtataaa aaacgaacaa aacacaagta
     3421 atgttgtaac cgctatattt atatgataag atgtctcaaa taataaaata gaaaagagac
     3481 ataaaaatga ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat
     3541 catcatcgag ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt
     3601 acaggtacag agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat
     3661 ctgactttta ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg
     3721 ctttctattg tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt
     3781 tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat
     3841 gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac
     3901 ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa
     3961 aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca
     4021 ttattagaaa gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa
     4081 caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt
     4141 actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca
     4201 gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga
     4261 ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg
     4321 atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata
     4381 gccactgcac atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca
     4441 gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct
     4501 gaaaagtatc aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact
     4561 gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta
     4621 ggtggttgta ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct
     4681 tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta
     4741 tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc
     4801 agctcgggga tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag
     4861 gatgtatacg aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt
     4921 ggtcatgtac cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa
     4981 gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc
     5041 tatcaaagac caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aaatgctgtc
     5101 agcttctttt tgtataacat attagaaagt ctaattatct ataactgtca catcgattaa
     5161 ctgtttcacg tcattgggat ttcctgtagg aatagctact acagatgcat tatcaatgat
     5221 ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca
     5281 atgtgaagga actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac
     5341 tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc
     5401 tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta attcgaaatc
     5461 acccgatgta tctctaaaag tattacctct aatagctagc caaacacgtg cgtactcaat
     5521 ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg aggaggttgt
     5581 gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt
     5641 gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg
     5701 agtacttgat ttttcagctg aactggtttg tctgattgaa cttttgtctg tggtcgcaga
     5761 tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa
     5821 taaaataaaa cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag
     5881 cataatcctg aatttttgaa tccgtaaaac cttgtctatg aatataaata gagtgtatct
     5941 tctttgataa atgtcagaaa aagtgtgaca aataatcatc agtaaaatta cttgtcacac
     6001 tattgataag gagtatttaa ttgaagagaa tgattaggag aaaggatctc cgtacttttt
     6061 atagtaatca tagaatccaa gtttgtatag acgaatcgtt ttttctctta tttcttctgt
     6121 ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct ataggagctg ttccattcgc
     6181 agtaatcaaa ttacgatctt tcacagcttg ttgttctata aaatcttctt tattctgata
     6241 attttccgat tctaaccaag gaaattgcgc atttccggtg tgtttgtaac cttgcaataa
     6301 tccgctctga gctaaataat caactgcccc acaaatagca gctactggta cgtctgtttt
     6361 taacaattta tcgattattt gatataaatg atgatttttt atgtcccatg aattgccacc
     6421 gatcataata aaaaggtcaa ctttttcagg tatttgttcg attaagtaat cgatttttgt
     6481 ttttaatcct ccaatggaag tgacctctga ttggatagaa gcagttttgt tttgccattt
     6541 ctcattttgg tttagtgtgc tagtaatata agcaccttcc caatcggcat attcctctaa
     6601 tagaaacgta atcgcagttt tcattttcaa gctctccttt actatctata acaaggacaa
     6661 cagttttaac ctacagacat tcattaaaat tgggtagcga cagcgaaaac aacaattctt
     6721 aagttttaga cagagtatac agatagtcaa cttaattaaa tacgaactaa ataaaaatga
     6781 caggtaatca tttttgttta ccagcattat aacattttgc atacatggat gacaaaagaa
     6841 ccaattccca cttgcttgag tacaatatta aaagaaactg gaaggcaaaa agtaattata
     6901 gaaacgaaaa ataaatcata tcgggcactc agacatgaaa acaaccctag agatatataa
     6961 ctaggtaacc agtttaatga aagaaaaaat catccaagaa gtcgattctt ggatttttta
     7021 gtggatttgt tcgccaaata cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt
     7081 gtggtctgag cacttaagga ggaatctgtg aaaatagctt ttcatatttt tgcagatttt
     7141 gacttaatgc cgaaggtcag ctttgcgatt accgtttatc gagaggctgt aaaagcaccg
     7201 tgtagctccg atgacaataa gcaagcattt ttagagctag gtttccttga ttctaaatat
     7261 gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac
     7321 gacttttcga cgtaaggaga aagaatttca atgatagaaa caataaatcc cgcagaaaac
     7381 aaggtttctg cgtacattca tatcccattt tgcgagcata tctgctatta ctgtgatttt
     7441 aataaagttt ttttagaagg gcagccagta gatgagtatg tggaaatgct gctgaatgaa
     7501 atgaaaataa tgctagaaag tcatccgaca gcgaatttag aaacacttta cgtagggggc
     7561 gggacaccaa cttctttatc tgctaaacaa cttgatcgac tgttatcagg tgctagagag
     7621 atcctgcctt ttcaaaatgg taatgagttt actgtagaag cgaatccagg tgatttgacc
     7681 cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc gtttatccat gggagtgcag
     7741 acatttgata atcgcttatt gaaaaaaatt ggacgtaaac acaccgcaga agatgtgtat
     7801 caaactatgc aatttttaga agcagaaaat tttaccaatg taagcatcga tttgatttat
     7861 gcattaccgg gacaaacact agaaggatat ctggatactt tggaaaaagc tttggctttg
     7921 gatctgcctc actattcttt gtactcattg atcctcgaaa acaaaacaat gttcatgaac
     7981 tgggtacgac aaggaagact cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa
     8041 gagacgattg aagcaatgga aaagcatgga cgtcaccaat atgaaataag taactttggg
     8101 ctctccggac atgaatctca acataacctc atgtactgga acaatgccca ctattttggg
     8161 tttggagctg gtgctagcgg ttatcttgga gatattcgtt accgcaacaa aggaccaatc
     8221 caacactatt tacgcccatt acgagcaaat gaacgcccgg tattggaaga agaaaaattg
     8281 acacgaaaaa atcaaataga agaagaaatg ttcttaggac taagaaaaaa aacagggatt
     8341 actaaaaaac atttctatga tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg
     8401 ctaagggaat tagagcaaga agggttactt gttaatgaag aagaccgtgt ttatttgaca
     8461 ccaagaggga cctttttggg gaacaatgtg tttgagcgct ttttgtttga aaaatagttt
     8521 aaagaatatt tgttttttat ctgaatttaa ataaaaaaag ttcaaaaata caataaagga
     8581 ctattgaaac gttgatataa ctggatttac atcaattatt tagaattagc actcttgtgt
     8641 ttagagtgct aaaaaatgga gtctttctct tgacaggaga agggcttctt gctatattta
     8701 taagtggatt agcacttaga atatatgagt gctaatgaga ggtgaggaag atgttaacaa
     8761 agcgacaaaa cgacatttta cgtctgatca tccaacacta tacgagtagt ggcgtgcccg
     8821 ttggttccaa aacattgatg tctgagggag tcaaggccag ctcagcaacg attcgcaatg
     8881 atatgaaagc attagaagat gaaggattgc tgttgaagac tcactcttct tctgggcgga
     8941 ttccatctgc tttaggctat cgttattacg tggatcattt gttaaaacct gctcgcgtag
     9001 cagaagatga tttgctgctg attcgtcagt ctctaggcag agaatttcat gaaatcaatg
     9061 agattattaa gcaatctgct gagatattat ctgagctgac aagctatacg actttttcat
     9121 tgggacctga aataaaagac cgccgactga ctggctttcg tatcgtaccc ttgaataatc
     9181 gacagattat tgctatcgtc gtaacagata tggggaatgt agaaagccaa gtgttcactc
     9241 ttcccgaata tatgggaagt caggaactag aaaaaatggt acagattgta aacgaccgtc
     9301 tagtcggaga tcctctgatg acggtgtacc ataaacttcg tacggagatt ccgatgattc
     9361 ttcataaata tttccaaacg acagaaggga tgtctcagtt atttgacacc gttcttggtc
     9421 atgtttttga agataaggtt tatgtcagtg gacaaatgaa tctcttggat tatgagcccc
     9481 gtcaagatct tgatcagttc aaatcgatgt tttcttttat gaaagactca gacggactta
     9541 cacaaatgat cactccgatg gatagcaata ttcatatcaa gattggtgct gaattaggca
     9601 atgatttatt gcagaatatg agtatgattc aagcaagtta cgagatagat ggtcatggaa
     9661 gaggtacaat agccttgtta gggcctacta gtatgcctta ttctcggatg ttcggtctag
     9721 tcgatgttta tcgaagagaa ctagcagcta aacttgctga ttactaccgt tcgcttgact
     9781 tgtcaaactc gtaatgtgaa taacgaagct atttgcagct gatcgttctc tctttcttag
     9841 taagagcaat caaacagctg actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc
     9901 aagaagaact agataaagaa atgacagatg ctcagccaga accggaaatc gatgtcgaag
     9961 ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga attcgaaaca gcgaaattaa
    10021 aagctgagtt agaagaaatg gaagataaat acttgcgggc tcgtgctgaa atcgcaaata
    10081 tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa ataccgttca caagatttag
    10141 ccaaaaaatt acttccatca attgacaacc tggaacgtgc attagccaca gaagtgtcag
    10201 atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg
    10261 cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc
    10321 ttcatcaagc agtacaaact gttccagcga ctgaagatac accagccgat acgattgtag
    10381 aagtactaca agaaggctac aaattacatg atcgcgtgtt aagaccaacc atggtcattg
    10441 ttgcacaata attgtgcgga tataaaactt acactttcaa ttaaagaagg agacgttaat
    10501 tatgagtaaa attatcggta ttgacttagg tacgactaac tctgcagtag cagtattgga
    10561 aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac cgtacaacac cttctgttgt
    10621 atcattcaaa aatggagaaa tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa
    10681 cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt
    10741 agaaggtaaa tcatatacac cacaagaaat ctcagcaatg atccttcaat atatcaaagg
    10801 attcgcagaa gactatttag gtgaaaaagt cgaaaaagca gttatcactg tgccagctta
    10861 ctttaatgat gcacaacgtc aagcaacaaa agatgctggt aagatcgctg gtctagaagt
    10921 agaacgtatc gtcaatgaac caactgcagc tgctcttgcc tatggtttag ataaaacaga
    10981 tcgcgacgaa aaaatcttag tattcgacct tggtggcggt acttttgacg tatctatcct
    11041 tgaactagga gacggtgtct ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg
    11101 ggacgacttt gataacaaaa tcatcgacta catggtagca gaattcaaaa aagaaaacag
    11161 cattgacttg tctcaagaca aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc
    11221 gaaaaaagat ttatctggcg tatcaagcac acaaatcagc ttgccattta tcacagcagg
    11281 agaatctgga ccattgcatt tggaaatgac attgacacgt gcaaaatttg atgaattaac
    11341 agctgactta gtagaacgta caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt
    11401 atcacaatca gaaatcgacg aagttatttt agtcggtgga tctactcgta tccctgcagt
    11461 tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac aaatcagtta accctgatga
    11521 agtagttgct atgggtgctg ctatccaagg tggcgtgatc actggtgatg tgaaagatgt
    11581 tgttttactt gacgttacac cattgtcatt aggtatcgaa acaatgggtg gagtgttcac
    11641 taaattgatt gaccgcaata caacgattcc aacaagtaaa tcacaagtat tctcaactgc
    11701 ggcagataat caaccagcag tagacatcca tgtattacaa ggtgaacgcc caatggctgc
    11761 tgacaacaaa actctaggaa gattccaatt gacagatatt ccagcagctc ctcgtggtat
    11821 tccacaaatc gaagtaacat tcgatattga taaaaacggg attgtaaatg tatctgcgaa
    11881 agatctaggt acacaaaaag aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga
    11941 cgaagaaatc gaacgtatgg taaaagatgc agaagcaaat gcagaagcag ataaagctcg
    12001 taaagaggaa gttgacctac gtaatgatgt agatgcattg ctattctctg tggacaaaac
    12061 attgaaagaa ttagaaggca aagtggacga agaagaagtg aaaaaagcag aaacagctcg
    12121 cgatgaattg aaagccgctg ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga
    12181 tgcattgaac gaaatcgttc aaaacttgac tgttaaacta tacgaacaag cggcacaaca
    12241 acaagctcaa gaaaacccag aagcagctca aggcggagca gatgatgtcg ttgatgctga
    12301 ttttgaagaa gtcgatggcg acgataaata atcagttcac tgaatttcga gagaaggcca
    12361 aagttgctac tttggctttt tcttttgaaa aatagtttga agtatcggtt tttgtttcaa
    12421 acaaaatatg atatgataac aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc
    12481 tatggcaaca aaacgtgatt attatgaagt cttagggctc tcaaaaggag catcagaaga
    12541 tgaaataaag aaagcttatc gtaagctttc gaaaaaatac catcctgaca tcaataaaga
    12601 acctgatgcg gaagaaaaat tcaaagaagt atcagaagca tatgagattt taagtgaccc
    12661 gcaaaaacgc gctgcatatg accaatacgg gcatgcaggc acagatccaa attacggtgc
    12721 tggtggagct ggtggcggat ttggtggatt cggcggcttc tctggtggcg gcttcggtgg
    12781 ttttgaagat atcttcgact ctttctttgg aggaggcggt cggtcagtcg atccaaatgc
    12841 gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg tcttttgaag aagcgatttt
    12901 tggtgtagaa aaagaaatca agtataatcg ggaagaaatc tgtcatactt gtggtgggaa
    12961 cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa tgtcatggtg caggtacgat
    13021 taatgtagaa cgccaaacac cacttggacg agtgatgagc cgtcaaactt gtgatgtttg
    13081 tcatgggaca gggaaagaaa tcaaagaacc ttgtccaacc tgtcatggct caggacatga
    13141 gaagaaagca cattcagtta aagtgaatgt accagctggt gtagaagaag gacaacaaat
    13201 gcgtttagct ggtcaaggag aagctggaga aaacggcgga ccatttggtg atttgtatgt
    13261 ggtcttccgt gtagaagaaa gtgatatctt tgatcgtgaa ggttctgaaa tttactatga
    13321 attgccttta agttttgtac aagcagcttt gggggatgaa gtccaagtac caactgtcca
    13381 tggagatgtg aaattgaaga tccctgctgg aacacagaca ggtacgaact tccgtttacg
    13441 tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa
    13501 actgatcaca ccgaaaaatc taaatgaaca acaaagagaa gcattacgtg cattcgctga
    13561 agctggcggc atcaaagttg aagaacagca agaagacggt ttcttcgata agtttaaaga
    13621 tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa aaacgctaag tcattaataa
    13681 ttaaaaacca gacgaacaat catatgttcg cctggttttt ttgtagttgt catgtttgta
    13741 ccaacaaaaa agccagcttt agaatatgat aatcgtcata gtttctaggg gtataaccag
    13801 tgagagaatg gaacttgttc aatcgatatt gcaaggtgtt tttatgaata aataattctt
    13861 ctgagcaatt tttgatgctt ccgttatttt tactgaagca tgccaaagtc ttcttgaaca
    13921 aatgtacttc ttcttctgtc aggcttttca aaatacgttt tacatataat gttcgttgat
    13981 ctgacgggat tgctggtaaa acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt
    14041 tcacagattg atggaaagct atccattcgc aaacttgttt ggcatgaagg aaagattgcg
    14101 gtgtctcttg ctctgtataa caaagcgacc caattccaaa gtttagctgt aactgataca
    14161 ttttattaaa ctttgtctgg attttatcaa gcaatgatag caatgaggct ctggaaatcc
    14221 gattgactag gataatgatt tgttgtctat gtacagtgag caaagcctgt ccgtaataag
    14281 agaagaccct ttcgagcaac tccaataaag gattgatccg ttcttgacac aattgctgat
    14341 tgtcagaaag ttgtcccaca actacataat atggagaaga aaaatcaagc tcaattaagt
    14401 gagggtggta tcgactatgt acttgatatc atcaatgaac cgatggtact ttcttatgcg
    14461 atggaacagg tagagcgatt gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga
    14521 ttaggtgaga aaataagaag ataaaaatac acggtccctt catggcgacc gtgcattttt
    14581 ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc attttgcgga
    14641 ttttcaaaga tttgttgagg cgttccatct tctaaaaagt ttccgtcgtc aatgaacatg
    14701 acgcgattgg ccacttcttt agcgaacccc atttcgtgcg taacgataac catcgtcata
    14761 ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc aggatcgagt
    14821 gcagaagtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc acgagcaata
    14881 gccacacgct gtttttgtcc accagaaagc atttcaggat acatatcttt tttatcttct
    14941 agaccaactg tctgcagtaa tttgattgct tttttctctg cttcttctct agattctttc
    15001 ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg gaataggtta
    15061 aagtgttgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt gcttttatcc
    15121 atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa acgattcaag
    15181 caacgcaaga atgtactttt tcctgaacct gaagggccaa tgatacagac gacatctcct
    15241 tcgttgatgg atacattgat gtcgtttaat actgtgttat cgccgtattt tttgactaaa
    15301 tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa cttttgctaa
    15361 ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt aaacatacgt
    15421 actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga ctacaccgat
    15481 tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga atgatgggat
    15541 catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat aagaaagacc
    15601 taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc ctcgaacaat
    15661 ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa ggaagtcagg
    15721 aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa gaaccatcat
    15781 tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag cgcgtattgg
    15841 tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa aggagatcaa
    15901 agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt tttggataag
    15961 tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg aaccatccgc
    16021 tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt tcaatccttc
    16081 attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg catattttcc
    16141 gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg cataaccgat
    16201 taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt cgtacaattg
    16261 atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt cgcttaaaaa
    16321 ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat cttcataaga
    16381 cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa agtaagaatc
    16441 agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa tcattccgtc
    16501 tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt ccattcggat
    16561 gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc cttcgtactc
    16621 gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa ttacgtatac
    16681 gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg ccgttgtagt
    16741 gttgtcagct tgtgcaacat gaccaaacgc aagtgtcaca agtcctgtca ttaagaaaac
    16801 aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc aatattttat
    16861 tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa tgaaaaaaat
    16921 gcgagtttct aacattttat gtta
//
LOCUS       DADVXN010000063        16754 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.065, whole genome shotgun sequence.
ACCESSION   DADVXN010000063 DADVXN010000000
VERSION     DADVXN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.065"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(40..753)
                     /locus_tag="KB942_001769"
     CDS             complement(40..753)
                     /locus_tag="KB942_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852191.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            928..2352
                     /locus_tag="KB942_001770"
     CDS             928..2352
                     /locus_tag="KB942_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6852192.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2651..3358
                     /locus_tag="KB942_001771"
     CDS             2651..3358
                     /locus_tag="KB942_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6852193.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3345..3668
                     /locus_tag="KB942_001772"
     CDS             3345..3668
                     /locus_tag="KB942_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6852194.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3757..4794)
                     /locus_tag="KB942_001773"
     CDS             complement(3757..4794)
                     /locus_tag="KB942_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6852195.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5115..6098)
                     /locus_tag="KB942_001774"
     CDS             complement(5115..6098)
                     /locus_tag="KB942_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBB6852196.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6513..7283)
                     /locus_tag="KB942_001775"
     CDS             complement(6513..7283)
                     /locus_tag="KB942_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852197.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7276..8211)
                     /locus_tag="KB942_001776"
     CDS             complement(7276..8211)
                     /locus_tag="KB942_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852198.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8527..10176)
                     /locus_tag="KB942_001777"
     CDS             complement(8527..10176)
                     /locus_tag="KB942_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6852199.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10293..12896)
                     /locus_tag="KB942_001778"
     CDS             complement(10293..12896)
                     /locus_tag="KB942_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6852200.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12908..13957)
                     /locus_tag="KB942_001779"
     CDS             complement(12908..13957)
                     /locus_tag="KB942_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBB6852201.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13983..14297)
                     /locus_tag="KB942_001780"
     CDS             complement(13983..14297)
                     /locus_tag="KB942_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBB6852202.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14313..14759)
                     /locus_tag="KB942_001781"
     CDS             complement(14313..14759)
                     /locus_tag="KB942_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852203.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14760..16682)
                     /locus_tag="KB942_001782"
     CDS             complement(14760..16682)
                     /locus_tag="KB942_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBB6852204.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4972 a         3408 c         2758 g         5616 t
ORIGIN      
        1 tataaaaaaa taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc
       61 ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga
      121 cgatagagca acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat
      181 ttttgcgtca aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc
      241 cagacccagt tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa
      301 gcctggtatg actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat
      361 aaaacggctt cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc
      421 tacttttttc gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc
      481 accaaccacg ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt
      541 tctgacgact tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa
      601 gtctacgcct ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc
      661 tgtaggaagt ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa
      721 gtaaatttcc atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc
      781 ttcatctata ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat
      841 aaaaaagata tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat
      901 taagtggaaa caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat
      961 gatttttggt ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga
     1021 aaagcagaga ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga
     1081 atcgattcct ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg
     1141 aaaaaaactg ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat
     1201 ggcggtaccg gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa
     1261 atgaataata acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact
     1321 cttcaagata aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct
     1381 gaaacttgtt ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca
     1441 cttgggccta ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt
     1501 aaacgcggag cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta
     1561 tttaaagaac tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt
     1621 cctcgtagtc aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg
     1681 cggagctttt tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta
     1741 caagaatata atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa
     1801 aataccgctc aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct
     1861 tctatggtgg atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg
     1921 cctggacgac gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat
     1981 ttgtgcatgg atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga
     2041 atgggcgttg cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat
     2101 cggattgagt atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt
     2161 aacattaagg ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat
     2221 aaaaaccgat atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa
     2281 agtggcgaat tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg
     2341 ttagttgaat aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat
     2401 gagaaatacc aataacaaat actttttctt catacttcat ttatacttgt cagtataaga
     2461 gactgtgaca aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt
     2521 tttttaaact acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt
     2581 aaaaatttag aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg
     2641 agttttgtta ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac
     2701 cgtttttggt tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt
     2761 tcatccgctg gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat
     2821 cacggtcagc atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct
     2881 agtcaattcc cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg
     2941 gctgcttcaa aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat
     3001 gaataaattg aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg
     3061 gatttcttat ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat
     3121 tactgatccg aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact
     3181 ttatcttcaa gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg
     3241 tctcactttg attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt
     3301 agttgtaaca ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt
     3361 ttacattgct gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat
     3421 ttgtcttact gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc
     3481 cgatcgtcat tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc
     3541 tgccgcagat caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc
     3601 tttcaaaaag cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct
     3661 tattataaat aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat
     3721 caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac
     3781 aacgactttt cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt
     3841 cgcattcatc ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc
     3901 taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt
     3961 tttagcgaac atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc
     4021 taacggctct ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt
     4081 gatttgatca aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat
     4141 tcctaattca cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc
     4201 tgtttttttc agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc
     4261 ccaatgcgct agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat
     4321 ttttttcccg cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc
     4381 ataagcggtc aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg
     4441 tgcaagagcg acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt
     4501 cgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac
     4561 agcaacaact ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt
     4621 atcttggcga agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag
     4681 atcccgtgaa ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc
     4741 gatcgacaac ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat
     4801 tcctccttag cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta
     4861 tgctatttgt tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc
     4921 acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt
     4981 gttcaatcaa ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt
     5041 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt
     5101 gttcaagcaa gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct
     5161 aagatctctc gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt
     5221 gctcctggaa atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc
     5281 gggatgattt ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt
     5341 tctttttctg tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc
     5401 cggccaatca tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga
     5461 tctgggatat ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca
     5521 atgattttag ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc
     5581 aaatggacag aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc
     5641 tcagacattt ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta
     5701 gccgcttcaa tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca
     5761 cgttctgcta cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt
     5821 tctgccatag caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca
     5881 accatcggct gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct
     5941 ggatgacaga aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca
     6001 tgctttacga catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa
     6061 aaaggctttg gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta
     6121 tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa
     6181 agaagcaaag tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa
     6241 aatcttatca caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa
     6301 taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg
     6361 atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg
     6421 tattagctca acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact
     6481 caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg
     6541 atcaaatacc aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat
     6601 gttgctactg gcacatcgat tgttccaagg aatccgtaac ggaaaccgga gatcatataa
     6661 acgatcggat tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag
     6721 aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg
     6781 tcataagact gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc
     6841 atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag
     6901 gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag
     6961 ccccaaatga tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg
     7021 aatttttgag agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc
     7081 ataatcaaac caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga
     7141 ccaccgatca tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc
     7201 agtgtttgga cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat
     7261 gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt
     7321 caaaaacagt tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg
     7381 tcttgtcagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat
     7441 tgtctgttca tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg
     7501 cgctaaatca aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt
     7561 attctcgatc agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc
     7621 ttctaaataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa
     7681 ctcccacatc tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag
     7741 caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga
     7801 aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc
     7861 gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat
     7921 ttgctggacc gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg
     7981 ctttgctcgg actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt
     8041 tttattgact aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc
     8101 taatagcgca tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac
     8161 cccacttcca taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct
     8221 atctccttat agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact
     8281 tggttccagc gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa
     8341 tcggacgaag tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc
     8401 atatgtgcaa caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
     8461 acacgtataa acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca
     8521 cttcgcctat cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt
     8581 tgcggtttct actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg
     8641 ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac
     8701 ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc
     8761 atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga
     8821 agaagcaatc cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca
     8881 gcgttctttg gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa
     8941 ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg
     9001 ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat
     9061 ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt
     9121 cttctgccag ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt
     9181 cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta
     9241 attcaatatt gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc
     9301 agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa
     9361 catgatgttg acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga
     9421 aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag
     9481 tggtgtcggc agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat
     9541 gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac
     9601 taacccaccg attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc
     9661 caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt
     9721 tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg
     9781 atattggatt ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa
     9841 ctgctgatct tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac
     9901 actagaatag gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt
     9961 aagatcatcg atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt
    10021 caagctttct tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac
    10081 tgtttccttt aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc
    10141 aataataatt aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt
    10201 tagctttagt atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa
    10261 taaaaggtgt ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa
    10321 taatcatagg gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc
    10381 ccaagggaaa ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga
    10441 agaacaaatc cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca
    10501 ggtaattcaa tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc
    10561 ttgttatcta acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt
    10621 tctaaccgtt tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca
    10681 ttaaatccaa aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg
    10741 acatgccctt catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt
    10801 tttccaagtt cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca
    10861 tactctttta aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca
    10921 ctcttatcga atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga
    10981 ggaatgacat caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca
    11041 gatttatcta aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt
    11101 ttgagtgtgc cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt
    11161 tcttccattt cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt
    11221 ccgtaaacaa ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt
    11281 agagatagtt ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct
    11341 gaatcgatca aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc
    11401 ccatctttaa aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt
    11461 gctgtttcta atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc
    11521 acatttacat ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa
    11581 cctgtagcaa tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg
    11641 atattatagg gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt
    11701 ttcgatccac tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc
    11761 tttaatgcat cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta
    11821 gcttctttga ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata
    11881 ctatcatgag cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg
    11941 atatggatac catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt
    12001 tgttccaatt taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga
    12061 acacgagagt aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt
    12121 ctgataactt ctacataaga aggataatct gaaataatat tgtggaattt actgtattta
    12181 ttcaacttct ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa
    12241 ggtactaaaa tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac
    12301 ttttcttttg caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata
    12361 taaccaaatg gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa
    12421 taaggagatg gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt
    12481 tctacttgat gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac
    12541 cctaccatca ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt
    12601 cttaaaattg attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg
    12661 aatagttgtc ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc
    12721 aaatactcat cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt
    12781 tccaattcat ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt
    12841 gtaaaatacc attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca
    12901 gattctccta ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg
    12961 attttagtgc attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat
    13021 ataaaggtat attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag
    13081 taacaccaaa aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta
    13141 ttactcgtaa aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc
    13201 ccataatcat agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta
    13261 aaattgttgc taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac
    13321 taggtccaaa gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga
    13381 taggtcctcc catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat
    13441 ttgctcctga catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg
    13501 tagctagtac ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca
    13561 taattgattt tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta
    13621 aatagccaca agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac
    13681 acatcatccc agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga
    13741 tataggctga aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga
    13801 ctccccagaa tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag
    13861 aagttagtaa ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat
    13921 gatttagaat ttctcttcct tgtttcttga attccattag taactccccc acgatatatt
    13981 ttttatttgt taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc
    14041 tctgaggtcc ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt
    14101 gtatctactg caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt
    14161 tctatcccca tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac
    14221 ttttctaaat tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact
    14281 gctactattt ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct
    14341 tttttatttc tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat
    14401 aattctgcta aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa
    14461 aagacgagat caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg
    14521 gctacagcca ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt
    14581 ttcccaattc ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg
    14641 tggtcagaca caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt
    14701 ttatcagatg catttaaatc tatcactata ttatttatat tgattttttc actgatcatt
    14761 tataagcctt ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt
    14821 gctaataact catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca
    14881 ggttttactt tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa
    14941 tcaattcctt ctggattaac gtagacacag attactggct tttttataaa atctacttcg
    15001 atatgcggca tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct
    15061 ccctctagta tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt
    15121 aaaacgttca cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat
    15181 aaatttagaa aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg
    15241 acatctgccc ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc
    15301 ggaatcgtgc ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt
    15361 agtacatctt ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat
    15421 tttgatgttc catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa
    15481 cgatccaaaa gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt
    15541 atacagaatc tctcttcgat ttcctctaat aaatctacac ttatcataaa tgacctttta
    15601 aaagaatatt tgattttgtc cgtataaggg ttatagatat ttactcccaa tttgagtctt
    15661 ttaactgctg cattgagatg taatactaga ttattgatca attcttcatc aggataaaat
    15721 tcatttaatt taattcttac taactcttgt agctcaacac ccatatctaa ataggaaagc
    15781 ttcgtttgat ctaagtcgtg attcatagca tttttttcaa tcgaagtaat gtgaatgtca
    15841 acataagctt gttcatctat tggcacaata attgaaaatg ctttttcaat cattttgatt
    15901 aaaataagtg tattctttga gatttcttct tcagaattat tactacgtga taagaaattg
    15961 cttgtttcaa tcctttctat cgcaatagca aggtggatag ctaaagtttg aaattcataa
    16021 tctgtaaatt tcaattgtgt cttattcatg tatcgattaa gcaattcggc tacttttttc
    16081 aatatgtcct tatttaagaa attattcgct tcaccaatga aattgatatt caagtctaag
    16141 agatctgttt tactgttaga tgctgtaaag ccaccccaat aatagttcac aacttctgac
    16201 atcaaccttc ttttactttt ttcatcagct ttgatgcgca cgccatttcg atctgaaact
    16261 aaggacatcc catgcttgtt tagtcttttt ctaacatctt tcattgtgct ttctaccgtc
    16321 gacctgctca cgtataattc gtcagcaaga ttttgaatct ttagagaatc attagtattc
    16381 aataatttag caaacacata agttagacgg tcctccggag agtctgcgat attgatttta
    16441 tgactggata ataaatttcg aagtgtttct ttttttccaa taagcgaaac gcctttttta
    16501 ggttgaacca acatttgtac atctggcatt ttttggatta tttctttcaa aattaaaata
    16561 tctttgctaa ttgttttcgt agatacatat aattgttctg ataattcttt gtaagttgtg
    16621 tgctcatttt caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc
    16681 attatcttca cctcctgaat acagcttaag tgtacaatta ttatctctat tttcatacag
    16741 aagaaagttc ttga
//
LOCUS       DADVXN010000064        16335 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.066, whole genome shotgun sequence.
ACCESSION   DADVXN010000064 DADVXN010000000
VERSION     DADVXN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.066"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1835)
                     /locus_tag="KB942_001783"
     CDS             complement(<1..1835)
                     /locus_tag="KB942_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6852205.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="KB942_001784"
     CDS             complement(1832..2536)
                     /locus_tag="KB942_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6852206.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="KB942_001785"
     CDS             complement(2548..4857)
                     /locus_tag="KB942_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBB6852207.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="KB942_001786"
     CDS             complement(5052..5516)
                     /locus_tag="KB942_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852208.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="KB942_001787"
     CDS             complement(5516..6142)
                     /locus_tag="KB942_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6852209.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="KB942_001788"
     CDS             complement(6149..6514)
                     /locus_tag="KB942_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6852210.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="KB942_001789"
     CDS             complement(6504..6842)
                     /locus_tag="KB942_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852211.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="KB942_001790"
     CDS             complement(6832..7158)
                     /locus_tag="KB942_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6852212.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="KB942_001791"
     CDS             complement(7139..7417)
                     /locus_tag="KB942_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6852213.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="KB942_001792"
     CDS             complement(7419..8783)
                     /locus_tag="KB942_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6852214.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="KB942_001793"
     CDS             complement(8796..9365)
                     /locus_tag="KB942_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBB6852215.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="KB942_001794"
     CDS             complement(9337..10524)
                     /locus_tag="KB942_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6852216.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="KB942_001795"
     CDS             complement(10588..12315)
                     /locus_tag="KB942_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6852217.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="KB942_001796"
     CDS             complement(12312..12764)
                     /locus_tag="KB942_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBB6852218.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="KB942_001797"
     CDS             complement(12876..13256)
                     /locus_tag="KB942_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6852219.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="KB942_001798"
     CDS             complement(13253..13639)
                     /locus_tag="KB942_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852220.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="KB942_001799"
     CDS             complement(13641..13919)
                     /locus_tag="KB942_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852221.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="KB942_001800"
     CDS             13967..14695
                     /locus_tag="KB942_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBB6852222.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="KB942_001801"
     CDS             complement(14826..15302)
                     /locus_tag="KB942_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBB6852223.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15609)
                     /locus_tag="KB942_001802"
     CDS             complement(15262..15609)
                     /locus_tag="KB942_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852224.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="KB942_001803"
     CDS             complement(15596..15781)
                     /locus_tag="KB942_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852225.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="KB942_001804"
     CDS             complement(15782..16054)
                     /locus_tag="KB942_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBB6852226.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16254)
                     /locus_tag="KB942_001805"
     CDS             complement(16051..16254)
                     /locus_tag="KB942_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852227.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4573 a         3453 c         2538 g         5771 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgta
//
LOCUS       DADVXN010000065        16123 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.067, whole genome shotgun sequence.
ACCESSION   DADVXN010000065 DADVXN010000000
VERSION     DADVXN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.067"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(250..1977)
                     /locus_tag="KB942_001806"
     CDS             complement(250..1977)
                     /locus_tag="KB942_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBB6852228.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1970..3163)
                     /locus_tag="KB942_001807"
     CDS             complement(1970..3163)
                     /locus_tag="KB942_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6852229.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3350..3994)
                     /locus_tag="KB942_001808"
     CDS             complement(3350..3994)
                     /locus_tag="KB942_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852230.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4088..4222)
                     /locus_tag="KB942_001809"
     CDS             complement(4088..4222)
                     /locus_tag="KB942_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBB6852231.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4224..6356)
                     /gene="feoB"
                     /locus_tag="KB942_001810"
     CDS             complement(4224..6356)
                     /gene="feoB"
                     /locus_tag="KB942_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBB6852232.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6353..6826)
                     /locus_tag="KB942_001811"
     CDS             complement(6353..6826)
                     /locus_tag="KB942_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBB6852233.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7227..7451
                     /gene="nrdH"
                     /locus_tag="KB942_001812"
     CDS             7227..7451
                     /gene="nrdH"
                     /locus_tag="KB942_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBB6852234.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7610..9769
                     /gene="nrdE"
                     /locus_tag="KB942_001813"
     CDS             7610..9769
                     /gene="nrdE"
                     /locus_tag="KB942_001813"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBB6852235.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9819..10784
                     /gene="nrdF"
                     /locus_tag="KB942_001814"
     CDS             9819..10784
                     /gene="nrdF"
                     /locus_tag="KB942_001814"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBB6852236.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10873..12012)
                     /locus_tag="KB942_001815"
     CDS             complement(10873..12012)
                     /locus_tag="KB942_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6852237.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12321..13034)
                     /locus_tag="KB942_001816"
     CDS             complement(12321..13034)
                     /locus_tag="KB942_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBB6852238.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13288..13989)
                     /gene="nagB"
                     /locus_tag="KB942_001817"
     CDS             complement(13288..13989)
                     /gene="nagB"
                     /locus_tag="KB942_001817"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBB6852239.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14223..15755
                     /locus_tag="KB942_001818"
     CDS             14223..15755
                     /locus_tag="KB942_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6852240.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15946..>16123)
                     /locus_tag="KB942_001819"
     CDS             complement(15946..>16123)
                     /locus_tag="KB942_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852241.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4989 a         3336 c         2826 g         4972 t
ORIGIN      
        1 tttaatattt tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca
       61 tacctacgat cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt
      121 tgaacgtttc gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg
      181 aacatggagc ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg
      241 tctgtctttt tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt
      301 tgctgcttgc tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt
      361 ttctgaccaa tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc
      421 ttttcgaacc atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa
      481 gtatttcaga tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg
      541 ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc
      601 tactttccag tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac
      661 accagctcct ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat
      721 cgaatttaca tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc
      781 acctaggtct tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag
      841 ccaagctgaa accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt
      901 atctaatggc gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt
      961 tgttgaagca acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt
     1021 ggcagatgaa aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat
     1081 gatcagtcct tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt
     1141 tggctggttc aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac
     1201 cccgttgaca aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc
     1261 taagccgggt gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt
     1321 ttgattgatc cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt
     1381 ttgacgactt gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg
     1441 aatccctaaa ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac
     1501 ccctggaacc atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg
     1561 cattgtctgc ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc
     1621 catcaagatc cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag
     1681 tgtgacagta ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat
     1741 taatgtgaac aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa
     1801 taacaagata aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac
     1861 tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc
     1921 tgtgatattt tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt
     1981 cactcacctc gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt
     2041 ttaattttcc atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa
     2101 tatcacttac caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca
     2161 atggacgttt ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt
     2221 gtctctttct gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg
     2281 caacgtttgg tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag
     2341 ccgtaagcac tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg
     2401 gattggtcaa tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg
     2461 cgattctgtc cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt
     2521 tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat
     2581 caagagccga gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg
     2641 ctaggccaac ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg
     2701 aaagcaaact agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta
     2761 caccacggac ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac
     2821 cgaagttttg gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc
     2881 ttaacttcga tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac
     2941 gattcaatag acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga
     3001 tttctccttc ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct
     3061 ttaaaatctc cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt
     3121 taccaaatat ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt
     3181 atgtaaattc atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca
     3241 ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt
     3301 gtaaataaaa ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa
     3361 gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa
     3421 aatgaacgat gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt
     3481 cattatgaga aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa
     3541 ttgaacggtc cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat
     3601 tcatcttatg tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat
     3661 tcagctcatc ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg
     3721 attgtacatc ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg
     3781 ccccacttcc atgccgtact tccatgattc caagatctac cagcacattg atagccttgc
     3841 gggtagtctc aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct
     3901 cacccacttt atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt
     3961 agcgcgggcg gacgattttt ttctttccag acattttctt tcctctttct ttttttactt
     4021 tccctcatct tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa
     4081 cgactgatca tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac
     4141 aattcttacc ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca
     4201 cacttaataa gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac
     4261 tggttcttgg attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata
     4321 gcaagatacg tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc
     4381 acaaaactaa ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc
     4441 ttggcattca tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa
     4501 ttgaaggcta acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag
     4561 atttctctgc cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata
     4621 atattttctt tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa
     4681 ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct
     4741 tcagaaacag gttgagcata aaaattataa gaagagctga accagatcac gatactagaa
     4801 acaaagataa tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt
     4861 aggaccgctg accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta
     4921 tctccagcaa acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata
     4981 ccaagaaaat aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact
     5041 aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa
     5101 cgacgatctt gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct
     5161 gtagcaatca gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga
     5221 tccatgacaa aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa
     5281 aacaatataa tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg
     5341 ttcaagatca gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca
     5401 tgttctggta accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct
     5461 actgtttgaa tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct
     5521 aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca
     5581 tttgacacaa cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga
     5641 atcgtctcac tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct
     5701 ttttgctgag aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat
     5761 ttcattgcat accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt
     5821 aaagctgctt ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt
     5881 gcagactcct gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt
     5941 ggtaaaactg gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct
     6001 tttttgatga catccatcat attcagacca atgatcatag gaatgtctaa atccaataac
     6061 tgcgtagtta aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt
     6121 tcaggctgtt cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac
     6181 aaagaataga tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct
     6241 tctttttttt ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag
     6301 agattgaaca agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt
     6361 tttcctccct ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc
     6421 ctcgcactgt cagttcgatt ggatctccca gaggggctag ttttcggata actattttta
     6481 catttcgcgt caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg
     6541 ctacgacgat cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg
     6601 aagcagtctt tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat
     6661 gactttttac cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc
     6721 cggcaacgat cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt
     6781 cgacttgata aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct
     6841 tgtgaaataa atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac
     6901 gcaaataaat gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat
     6961 acttgtgata ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt
     7021 cttcatagtt ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg
     7081 ttttcagctc ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata
     7141 tgtagtgttt gttgtttgac ttttttacta agagttttat actatctatt atagataata
     7201 agaatgtgtt agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta
     7261 tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca
     7321 atatcgatga acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc
     7381 ctgtcattac ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac
     7441 ttgccagcta acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga
     7501 cagcaccagt ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat
     7561 gatttgcaat caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa
     7621 aaatttacaa gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg
     7681 tcaaatccct ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa
     7741 accaaatacg atgacttttc catctattat ggaaaaaatc aattacttga tcgatcaaga
     7801 atatatagaa gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca
     7861 atttttggac gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc
     7921 tcaatatgcg ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt
     7981 agccttcaat gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga
     8041 cgaaatgatc catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa
     8101 acgtcggggc gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat
     8161 cgggcgctca atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac
     8221 tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg
     8281 tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca
     8341 gcgtcaagga gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct
     8401 ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt
     8461 gatcgtacct gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag
     8521 tccatatagt gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga
     8581 gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct
     8641 tgaaaatgaa atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga
     8701 tacagcgaac cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga
     8761 gattttacaa gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg
     8821 aacagatatc tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa
     8881 ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga
     8941 tatcgatgtc gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg
     9001 tgccatgggc ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc
     9061 attagacttt acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa
     9121 caatatcgct cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga
     9181 cggcacttat ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa
     9241 agcattgttt gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc
     9301 agtaatgaaa gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat
     9361 ttcatatatc aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg
     9421 ccaagagaag aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat
     9481 tccgtattac acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaac
     9541 ggcacaacaa cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc
     9601 agaaggcatt tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt
     9661 aagacattac gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga
     9721 tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata
     9781 gattcgctta ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag
     9841 caataaactg gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag
     9901 agcaattttg gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat
     9961 tgtctcaatt ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata
    10021 ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag
    10081 aagccgtttt gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta
    10141 tttttagtac actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga
    10201 atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc
    10261 tagaaaagaa aattgccagc gtctttttag aaacattctt gttctattca ggtttttata
    10321 ctcctcttta ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa
    10381 tcatccgaga tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca
    10441 acgagttacc tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg
    10501 agctttacga aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag
    10561 aagaagtcaa gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg
    10621 atccactctt tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa
    10681 caggcacaag caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg
    10741 tagaagcgat gaaagacgac gattatttga ttggattgga ataaggattt attttaattc
    10801 aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac
    10861 tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga
    10921 aacatgttcc attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat
    10981 cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc
    11041 aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg
    11101 cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc
    11161 ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc
    11221 gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg
    11281 atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt
    11341 actgctgata taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt
    11401 acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa
    11461 cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga
    11521 aatgattttt gagacacttg cagataaact gactaatatg atattgaaca aattactaag
    11581 agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg
    11641 cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa
    11701 ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat
    11761 gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag
    11821 aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa
    11881 aattgccgaa agcattttca atattggact aaaaatattc ggcatttgta aaataaagta
    11941 cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa
    12001 acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa
    12061 gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa
    12121 tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca
    12181 gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa
    12241 acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct
    12301 attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt
    12361 ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc
    12421 gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct
    12481 tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta
    12541 tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact
    12601 gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg
    12661 atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc
    12721 gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga
    12781 gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca
    12841 tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca tgctcccata
    12901 caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg
    12961 attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat
    13021 accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca
    13081 aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt
    13141 cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca
    13201 aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc
    13261 acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat
    13321 cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc
    13381 ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc
    13441 tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc
    13501 tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt
    13561 cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc
    13621 taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc
    13681 ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa
    13741 taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta
    13801 ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt
    13861 atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat
    13921 accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat
    13981 gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt
    14041 tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat
    14101 aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac
    14161 caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta
    14221 ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg
    14281 aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag
    14341 ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga
    14401 tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa
    14461 cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa
    14521 aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag
    14581 ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg
    14641 ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc
    14701 taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg
    14761 ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc
    14821 agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg
    14881 atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc
    14941 atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc
    15001 atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt
    15061 cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc
    15121 ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat
    15181 tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca
    15241 atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat
    15301 ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc
    15361 cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa
    15421 caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc
    15481 aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg
    15541 gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga
    15601 ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga
    15661 tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc
    15721 ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa
    15781 aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag
    15841 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta
    15901 ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc
    15961 gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac
    16021 aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa
    16081 tgaaccagcc gcacctccaa taaagtacat aaacataaat aca
//
LOCUS       DADVXN010000066        15985 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.068, whole genome shotgun sequence.
ACCESSION   DADVXN010000066 DADVXN010000000
VERSION     DADVXN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.068"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            226..894
                     /locus_tag="KB942_001820"
     CDS             226..894
                     /locus_tag="KB942_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBB6852242.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="KB942_001821"
     CDS             complement(1094..1324)
                     /locus_tag="KB942_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852243.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="KB942_001822"
     CDS             complement(1451..2833)
                     /locus_tag="KB942_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6852244.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="KB942_001823"
     CDS             complement(2989..4176)
                     /locus_tag="KB942_001823"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBB6852245.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="KB942_001824"
     CDS             complement(4183..5214)
                     /locus_tag="KB942_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6852246.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="KB942_001825"
     CDS             complement(5260..6684)
                     /locus_tag="KB942_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852247.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="KB942_001826"
     CDS             6859..7764
                     /locus_tag="KB942_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6852248.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="KB942_001827"
     CDS             7855..8532
                     /locus_tag="KB942_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6852249.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="KB942_001828"
     CDS             8529..8699
                     /locus_tag="KB942_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852250.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="KB942_001829"
     CDS             8813..9709
                     /locus_tag="KB942_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6852251.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="KB942_001830"
     CDS             complement(9764..11134)
                     /locus_tag="KB942_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6852252.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="KB942_001831"
     CDS             11372..12118
                     /locus_tag="KB942_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6852253.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="KB942_001832"
     CDS             12115..12954
                     /locus_tag="KB942_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6852254.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="KB942_001833"
     CDS             12955..13905
                     /locus_tag="KB942_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6852255.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="KB942_001834"
     CDS             14046..14798
                     /locus_tag="KB942_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852256.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="KB942_001835"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="KB942_001835"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBB6852257.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5190 a         2995 c         3221 g         4579 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa catta
//
LOCUS       DADVXN010000067        15558 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.069, whole genome shotgun sequence.
ACCESSION   DADVXN010000067 DADVXN010000000
VERSION     DADVXN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.069"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(12..482)
                     /locus_tag="KB942_001836"
     CDS             complement(12..482)
                     /locus_tag="KB942_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBB6852258.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="KB942_001837"
     CDS             complement(641..1099)
                     /locus_tag="KB942_001837"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBB6852259.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="KB942_001838"
     CDS             complement(1270..2415)
                     /locus_tag="KB942_001838"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBB6852260.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="KB942_001839"
     CDS             complement(2428..3345)
                     /locus_tag="KB942_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBB6852261.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="KB942_001840"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="KB942_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBB6852262.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="KB942_001841"
     CDS             4437..4607
                     /locus_tag="KB942_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852263.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="KB942_001842"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="KB942_001842"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBB6852264.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="KB942_001843"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="KB942_001843"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBB6852265.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="KB942_001844"
     CDS             6871..7752
                     /locus_tag="KB942_001844"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBB6852266.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="KB942_001845"
     CDS             7749..8465
                     /locus_tag="KB942_001845"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBB6852267.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="KB942_001846"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="KB942_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBB6852268.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="KB942_001847"
     CDS             9182..10183
                     /locus_tag="KB942_001847"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBB6852269.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="KB942_001848"
     CDS             10180..11541
                     /locus_tag="KB942_001848"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBB6852270.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="KB942_001849"
     CDS             11501..12652
                     /locus_tag="KB942_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBB6852271.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="KB942_001850"
     CDS             12627..13796
                     /locus_tag="KB942_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6852272.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="KB942_001851"
     CDS             13971..15350
                     /locus_tag="KB942_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6852273.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5077 a         2754 c         3310 g         4417 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactt
//
LOCUS       DADVXN010000068        15469 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.070, whole genome shotgun sequence.
ACCESSION   DADVXN010000068 DADVXN010000000
VERSION     DADVXN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.070"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            103..381
                     /locus_tag="KB942_001852"
     CDS             103..381
                     /locus_tag="KB942_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852274.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            400..1608
                     /locus_tag="KB942_001853"
     CDS             400..1608
                     /locus_tag="KB942_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852275.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1756..2121)
                     /locus_tag="KB942_001854"
     CDS             complement(1756..2121)
                     /locus_tag="KB942_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBB6852276.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2268..3041)
                     /locus_tag="KB942_001855"
     CDS             complement(2268..3041)
                     /locus_tag="KB942_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6852277.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3215..4075)
                     /locus_tag="KB942_001856"
     CDS             complement(3215..4075)
                     /locus_tag="KB942_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBB6852278.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4075..5493)
                     /locus_tag="KB942_001857"
     CDS             complement(4075..5493)
                     /locus_tag="KB942_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6852279.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5550..5999)
                     /locus_tag="KB942_001858"
     CDS             complement(5550..5999)
                     /locus_tag="KB942_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852280.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6444..6707)
                     /locus_tag="KB942_001859"
     CDS             complement(6444..6707)
                     /locus_tag="KB942_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBB6852281.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6806..8101)
                     /gene="rho"
                     /locus_tag="KB942_001860"
     CDS             complement(6806..8101)
                     /gene="rho"
                     /locus_tag="KB942_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBB6852282.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8098..9369)
                     /locus_tag="KB942_001861"
     CDS             complement(8098..9369)
                     /locus_tag="KB942_001861"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6852283.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9534..10403)
                     /locus_tag="KB942_001862"
     CDS             complement(9534..10403)
                     /locus_tag="KB942_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBB6852284.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10695..12305)
                     /locus_tag="KB942_001863"
     CDS             complement(10695..12305)
                     /locus_tag="KB942_001863"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBB6852285.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13498..14838)
                     /gene="glnA"
                     /locus_tag="KB942_001864"
     CDS             complement(13498..14838)
                     /gene="glnA"
                     /locus_tag="KB942_001864"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBB6852286.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14866..15258)
                     /locus_tag="KB942_001865"
     CDS             complement(14866..15258)
                     /locus_tag="KB942_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6852287.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4395 a         3289 c         2651 g         5134 t
ORIGIN      
        1 acatttgtta agtacctttt tccatcactt atgcgatttt tttccttttt tttattcgtt
       61 atactgcaga agtaaaattt tttaagaata gagggaaaaa acatgaatta ctggatgaac
      121 acaatcatta atcgattaga aacagcatat caaacaagat ttgatatgaa agcttcttta
      181 gtctttttaa atgatgcgta tcaaaactcg atcgaattga tcaaagcagt ggatgagaat
      241 ccaacaaacg aatgcgaaga atttttaaat ctgtttatgt ctacaagaga tttatttatt
      301 cgccaacttg ttgatcgcta tccatccaat tatcacgatg tagaagttca aatacaaaaa
      361 ttaaaagcat attcagctta aggggaatgt ataacataca tggaaaagaa attatttaat
      421 aaatatttta ttggaatcac attgatcaat tttattgtct acctgattta ttatctgttg
      481 atggtcatca tggcagtcat tgcgcaagac accttacacg ccagcttgag ccaagcaggt
      541 tttgcttctg ggatctatat catcggaaca ttgctggcac gcttattcat ggggaaacag
      601 ttagaactat ttggtcggaa gtttacctta agaggcggcg cgatcttcta tctcctttca
      661 acattagcat atctttatac gccaactatc ggggcattgt atttagttcg attcttgaat
      721 ggattcggct acggtacagt ttctacagct acaaatgcta tcgttactgc atatatccct
      781 gcatctaaaa aaggggaagg catcaattat tatggattaa gtactagttt agctgccgct
      841 gttgggccat ttttaggaat gattttgctg aatatgactg attttcgttt cattatctgg
      901 ttttcaattg tactggtctt ctttgttacg attgcctgcc ttgtctttcc tgtaaaaaat
      961 attccattat ctgaagaaca aaaaatatct cttcgctcat ggaatatcaa tagttttatt
     1021 gaaaagaaag cacttttcat tacaggaatt gcctttttga tgggtctttc ttattcaagt
     1081 gttttatcat ttttatcttc ttatacaaaa gtaatccact tagttgctgc tgggtcattt
     1141 ttcttcgtcg tatatgcttt ggtcatcaca tttacacgtc cattgactgg aaggattttc
     1201 gatgtcaaag gagaacaata cgtgatgtat cctagctatt tattcttaac agcaggatta
     1261 tttttactga gtgtaacgac gaatagtgtg acattgctta tctctggagc attagtcggc
     1321 ctaggttatg gtacgtttat gtcaaatgga caggctgttt gtttaaaaat cgtccgcgaa
     1381 catcgaatca gtattgcttt atctacctac tttattggtt tagacctagg cttaggcgtt
     1441 ggtccttatt tgatggggat gctaaaatct cttacttcat ttagaggatt gtatgtgatt
     1501 gccggagtca ttccattgat atgtacagca ctctatcttg ttaatggccg aaaacatagt
     1561 catgaagcag agggcgaatt gattgaatca ttgaaaaatg aaatttgatc ttgatgaata
     1621 gataaaaaaa accgttcaga tctgaaaata atctgagcgg ttttttgagt aaaaatagtt
     1681 tatgattatt cttaattaaa catctattta tttttaaagc tttcatttat cgataagaat
     1741 agtttaactg ttgatttatt ccattttccg ttcatctggt acgatgcctt gacttagcaa
     1801 ccacttgtgg aagaaccatt ctgccacacc gatcaataga gcaggaatca atactttcat
     1861 ccaaatatcg actgtatcat tcataaaata tagatagagc cacaagataa ctgcagctga
     1921 accagcatct gcgatcgtcg ctgcgatgtt ccctacttta cgtaagatca acagatcccc
     1981 tagcacatag atcatgacta caataattac cccaacaatc gttgaatcca taaagctaac
     2041 accgtataaa cctgtcagca cgacccacag aatcgcccag atcatgattg ccttcactac
     2101 caacgccttc acatgtttca ttttctgcac tcctttctcc tggataaaaa agtaagatta
     2161 tctaacaaga taaccttttc attattatac catcggctga gagaagcgag caaagataag
     2221 gtcctatcta aaaatgagga agccacttag aacattcacg taataattta tccgtacaga
     2281 cattcgatat ggtgttcctt tactttcgca tactgttctt ttgtcagttc tttacttgtc
     2341 accagataat cgatttcatg aaaagcaatg gcttgatgca tgaatttatg tgcaattttt
     2401 tgctgtgtaa ctaaaacggc aagccggtct gattgttttc taaaagcttt tttgaccatt
     2461 gcctctcctt catgagtttc tgttactcct tggctgaaat ctagcccgcg acaactcatt
     2521 atcgctaaat ccgcataata tgacaatgca tcgttatagg cttttgaacc gttgattgtt
     2581 gctcgtttcg gaacaattgc ccctcccaat acagatactt ttgccgttgt atattcactc
     2641 aacatagcag ctgtgacgat gccattcgta atgatctgca aaccatcaaa tcttgctagg
     2701 tttctgacta agtgtaaaga ggttgtactg gaatccaaga ataagaccat atcatctcga
     2761 atcaaagaag aagctgtatc tgcaatctta cgtttttctg tttcttcttc taatttggtg
     2821 atcttcatca ggtaaggcac gtcaattttt cgattgtcaa taagcatggc gccaccgcga
     2881 actctacgga tcaatccact tttttccaga aaaacaaggt cacgtttaat cgtggcttcg
     2941 ctataattta ctaactcgat caaatcggtg actcttgcaa attggttgtt ttttagataa
     3001 ttcacaatct cctgctgtct attctccact tcattcaaca taaaatcacc ccatttactt
     3061 tatatagtaa caggaaaaaa gaggttgtga cagaagtgat tggcttcgag aaataagaag
     3121 aaatttttcg aaaaaactac ttctgaacat cgtgtgtggt taagagggta tgacaagact
     3181 attgtcatac cctcttgacc acacattttt atttttagta taaactagcc ttgcctactg
     3241 tgttcaaaat ttccatttta tgtttgacca cttcttgtac ctttttatta gctgctggat
     3301 aaattgcatt cggctcgtac atcgatggat tttcactcaa tatttctctc aaagcattga
     3361 aaaagacgct tttaagatct gagctcaaat tcactttggc aactccgtat tttactgctt
     3421 cgctgacttc tgcgtccgga ttacctgaac cgccatgtaa aacaaatgga agatcgattt
     3481 gttggttcaa ttctttcagt aagtcgatat ttaattttgg agttctgttt ttcggataca
     3541 agccatgcgc tgttccaatt gctacagcaa gtgtatcgat tcctgtttct ttcacaaaaa
     3601 tcttcgcttg atcaggatct gtataaatga ttgtttcagc acctccttca gcagaaccat
     3661 tattcccaat cgttccaagc tcagcctcca ctgagacatt taccgcatga gctaacgcaa
     3721 ccactttttt cgtaatctgt acattgtcgt cgaatgattt caaagacgca tcgatcatca
     3781 cggaagaata gccgtttcga atcgcccgta agacatcttc tatcgtaccg ccatgatcca
     3841 agtggataac gatcggcagt ttacttgtat aagcataggc tcttactgcc gcaacaaact
     3901 catcacttaa ataggcgatt tcatcaggat gaatttctaa gattactggt gcttctaatc
     3961 tctctgcttc ttcgatgatt gctttcatca tatcatagct gcaaatattg aaggctggta
     4021 ctgcaaattg attctcttta gccactttca ataactcttt cattgtatat aacattacgc
     4081 ttccacctca tttgttagat tttcattttt cgtccacttg tcttgttttt tctctttcac
     4141 tgtttttttc aatagtccaa gcataaaggc tgtagtgact gtacctgatg caattgccaa
     4201 gacatacata ggtaaattgt tcataacatt tggaattagc atcacccaaa tccctccatg
     4261 aggtgccatt gaagtaactc ctactgccat ggaaatccct gcagcgactg ctgaacctgt
     4321 catcaaacta ggtatcacgc ggatcgggtc agataccgca aacggtatag caccttctgt
     4381 aatatacgaa gcgcctaaaa tccagcatga ttgacctgaa gtccgttctt ctttggtaaa
     4441 tttatgtttg aataataaag tggctaaagc aatacctagg ggaggtgtca ttcctgcagc
     4501 catacaagca gcaatcggtg catttactcc agctgacatc aaaccgatcg agaaagtcga
     4561 agtcgattta ttgatcggcc cacccatatc agctgccatc atgatcccga taagtaatcc
     4621 gaacaatagc ccggatgtat ttcccaagct attcagccaa tttgtcaaag catccaacac
     4681 ccatgtgatc ggttgttcaa tcacaaatac catcaaaagc ccaataatta agatcgataa
     4741 aaaggggacg acaatcagct gataaatagc agaaaattct ttttttacgc taattttatt
     4801 tgccagaaaa tcacagatat accctgctag cacgccacca atcattccgc caagaaaacc
     4861 tgaaccacct gtagaggcca gtaatccggc aacaattcct ggggcaaaac ctgcttttcc
     4921 agcaatcgat gtagcaatcc ccgcgcctaa cgcaggaacc atcatggcaa acgctgtatc
     4981 tcctccgatc cttgaaaatg cagctgctaa cacattatag tctgctgagt tgggatcagc
     5041 cgctgtaatc ccaaaggcaa aactcagtgc gatgatgatt ccgcctgcga tcacaaatgg
     5101 aagcatgtaa ttcacaccat tcatgaaatg attatagatt gtcggctttt tctcatttgc
     5161 agctggttct ttttcattag acacaacgaa tacgccaata tctctcaatg cttcgtctat
     5221 tactacttgc ggttctttga ttgctcggct tgtagagaca cgctttactt ttttcccggc
     5281 aaatcggtcc aattcgattt ctttatctac tgcaagaatg attgcatctg cctctttgat
     5341 ttcttgttca gataactgat tttctgtttt tacaccttgt gtttcgaatt ttgcttgatg
     5401 gtttagattt cttgctgtta cttctaattt ttctgccgac atataagtat gagcaattcc
     5461 agtcgggcaa gcagttacac caacgatttt catgattatt ttcctccttt ttcttcacta
     5521 tcgatactcg gacaaagact ctactatttt cattcttgac taatctgtct gatgatatct
     5581 gctggcgtag tggcttcttt tattcccttt ctaaaatctg cattcatcaa tcgtctggag
     5641 agtgtgctga gaatctctag atgcatatct gctgattttt caggaactac gatggcaaaa
     5701 atatattcaa ttggcatttc atctatcccg ctatagtccg ttaatttggc tgtatgaaaa
     5761 aaacacagtg ctggttttgt cacatatttg ctttttgcat ggggaatccc aaatccatct
     5821 tctattcctg tggagatctc ctcctctcgt ttgaatagtg ccttaacgac tttttgaaca
     5881 tttcttacct tatttgtttt tttcaacaca tcccctattc gctcaaataa tgatcctttt
     5941 gaatctatcg atggatcgaa aacaatcagt tcttctgcga tcaattgatc aaacctcata
     6001 aaaaacctct cccccacaaa aaatgttaac gttttcttgg aactgaggtc agtatagcat
     6061 cgctagctgt accgttcaat attatgaatg aacagaggaa aaagacccat catccgcgtg
     6121 tataagtcat taaaatgaac gatctagctc acaaacaatc gcaagtctat aacaaaacaa
     6181 aaagccttcc ttgaaaggaa agcttagctt ctaattttag gtaaatggtt ttttacttcg
     6241 aaataacccc tcttcatttc gttaatataa ggtgttttta tcaagtggat aattaaaact
     6301 atatactagt ttttcattgt tttgtgcatt atgatcctac gactttttca gcatcaaacg
     6361 aagttttaaa ttggcaaaaa acataaaaaa acagactatc cctatcagat agtctgctaa
     6421 acgttgagtt atcagcgttt ttattattcc gcagcgtttg cgtctttgag accgtatttt
     6481 ttgttgaaac ggtccacacg tccgtctgct tgtgtgaatt tttgacgtcc agtgtagaat
     6541 ggatgtgagt cagaagttac ttcgacacgg atgactgggt atgtattacc gtcttcccat
     6601 tcaactgttt cttgtgaagt ttttgttgaa cctgacaaga atttgaaacc agttgttgaa
     6661 tccatgaata ctactgggtg gtaatctgga tggatgtttt ctttcatgat tttttcgctc
     6721 ctttgccctg ctccatttgc tgaaccagag ttgatttgtc gatttacaac attaacatct
     6781 taacatgttt cagtcaaggt tgcaattatc ttttcagatt tctggtcgga ttttttttac
     6841 caaaggagac atcttgaaat tctttgaaaa attgctgatt gttttttgtt ttacgcaaaa
     6901 agcggatcag ttgttccgta tattctaatg aatctccagt catatttttt cgaagtttcc
     6961 aaatttcttc taattcttca cttggcatca ataattcttc ttttcgggta cctgattttt
     7021 tgatatcgat ggctgggaag atccgacgtt cagataattc acgtgaaagt tgtaattcta
     7081 gatttccagt tcctttgaat tcttcataga tcacgtcatc catacggctt cctgtatcga
     7141 ctaaagcggt cgctaaaatc gtcaaactgc caccttcttc gatgtttctt gctgccccga
     7201 aaaatttctt aggtttatac aatgcggctg ggtcaatccc gccactcaat gtacgtccgc
     7261 ttggtggaac gactaagtta taggcccgag caagtctagt aatactatcc atcaagataa
     7321 cgacatctcg tttatcttcg actaaacgca ttgcgcgttc caatactaat tcagaaactc
     7381 gagtatggtt ttgcggctgc agatcaaatg tagaagatac cacgtcgcct ttcacgcttc
     7441 gttctagatc cgttacttct tccggtcgtt catcaatcag tagaacgatt aattctactt
     7501 ccggatagtt ttcggtgata ccatttgcga tttcttttaa tacagaagtt ttccctgctt
     7561 ttggcggtgc gacgatcaat ccacgctgac caaaaccgat tggagaaaag atatcgatca
     7621 ttcgggtaga tagtctttgc ggtgttgttt ccagttgaag ctgtctttgc ggataaagag
     7681 gtgttaaggc tggaaaatgc ggacgttctt ttgcttcttc cggatctttt ccattaacgc
     7741 tttccacgtg catcaaacca taataacgtt ccgattcttt aggtggacgg gcttttcctg
     7801 ccactttgtc cccatttctc aaactgaatc gtctgatttg tgaggcagaa atatagatat
     7861 cttctgcact tggtccataa ttgattggcc gtaaaaaacc gtaaccatct tgaccaacga
     7921 tatccaatat tccttccata taataaaatc cttgtttctc tgcttgtgca cgaatcacag
     7981 ctagagataa ttccttttta ttcatcttac tgtaataagg aattttaaat tctttggcat
     8041 aactataaac ttcttttaac gttttgttct ctagttctgc cattgtcaaa taatcgctca
     8101 ttcagctccc acttcctcta tcatttcaat tgatgcccct aaggctgtta gtttctcgat
     8161 aatatgatca taaccgcgta agatataatc tacattgtaa atcgtcgttg tcccttctgc
     8221 caatagtcca gcaatcacta aacaagcacc tgcccgaaga tcagaagcca cgacttctgt
     8281 gccgtgaagt ttatttggac cattcacgat gatcatattt ccttccacgg taatatcagc
     8341 gcccattcgt gctagttccg gcacatggtt cacacgtttc gcatagatcg tatcgatgat
     8401 ttctgaagtg cctgtagtca tcaatagtaa cgcagtaagt ggctgctgca gatctgtggc
     8461 aaatcctgga taaggatagg tcatgatgtt tgcgggtttt aattctttcg aggggtaaac
     8521 ttcgatttca tcttcgctga ttttcatatg gacaccaatt tcctgcaatt ttgctataaa
     8581 gctttccaaa tgttcaaaga tcacattttt cactttgata ccatttccta ctgcagctgc
     8641 taatgctaga taggttcctg cttcgatgcg gtcaggaatc atgaaatgcc gacagccatg
     8701 taatgtttcg actccttcga tacggatcac atctgtaccc gctcctcgaa cttttgcccc
     8761 catattattc agtaaagttg ccacatctat gatttccggt tctcgtgcag cgttttcaat
     8821 tactgtctgt ccttttgctt ttacagcagc taacatcaca ttgatcgttg ctccgactga
     8881 aaccacgtcc atgaaaatcc gattgccacg caatccttta ttttctgtcc gtaaatacat
     8941 ggcaccatgt tcatttgtca cttcagcacc tagagcttca aatcctttga cgtgtaggtc
     9001 aatcggtcta gggcctaaat aacagccacc tggcaggcct actaccgctt cgccaaattt
     9061 accaagcagt gttcccataa aataataaga agcacgcaaa ctattgattt ttccgtgcgg
     9121 catcggaatc gatacgactt ctcgaggatc gatttctagt atgttgttgg aaaaatggac
     9181 agttgcgccc atgatctcta aaatttcaat taatgaatga acatcttgta tgtccggcac
     9241 accttccaag atcactgggg aatcagctaa gattgcagct ggaatcaatg cgactgcact
     9301 attttttgca ccgctgatcg ttacttcacc agctaaaggt cggtttcctt tgattacgat
     9361 ttttttcatc tgtttccacc ttgtttatta atcttttttc atggatatgc ttgtatttta
     9421 tcatagataa aaaaaagaaa cgagcatggt ctgccgtttt aagtaaaaaa gaatggagag
     9481 cgacttcaaa agtcgttctc cattcctata tatcgtatct gttatgtgga tgcttacgct
     9541 ttgttagctg aaccgaacca gtcgatatga gatttagcat cttcaacgat tgctgcttta
     9601 cctggagcta ataatttacg agggtcgaat cctttacctt ctttatcttt accagcttcg
     9661 atatatttac gagtagctgc agcaaatgat aattggaatt cagtgttaac atttactttt
     9721 gagataccca ttgagatagc tttttcgatt tgatcttgag gaatacctga tccgccatgt
     9781 aatactaaag gcatgtctgg acctacagca tcagcaattg cttgtaagtg gtcaaaagct
     9841 aatcctttcc agttttctgg atattgacca tggatgttac cgataccgca agctaagtag
     9901 tcgatacctg tagcaaccat ttgtttgcat tcttcaatat cagctaattc gccgttaccg
     9961 atgattccgt cttcttctcc gccgattgaa ccaacttcac attcaactga gatacctttt
    10021 gcatgtgctt tttcaacaac gtctttagct aatttcaagt tttcttcaaa tggtagatgt
    10081 gaaccatcga acattactga agtatagcct aattcgatac attccaaagc agcttcgtaa
    10141 tcaccatggt ctaagtggat agcaactgga acagtgatgt tcattgcttc aactaaatca
    10201 gtgattaaat ctttacagat tttgtaacca cccatgtatt tagctgcacc cattgatgtt
    10261 tggataagta ctggtgcttg ttttgcttca gccgcttcaa ggattgcttg agtccattcc
    10321 aagttgtttg tgttgaatcc gccgacagcg taaccgcctt tacgagcagc ttttaaaaat
    10381 tcagctcctg atactactgg cataataaaa ttcctcctaa gttttgtaaa actaaatttg
    10441 ttaagacaat tcttaaccaa cgaccctatt ttaccaaaca tttcggtagt tttctactaa
    10501 aatcaatgaa aatgaaaatt ttcaagaagt tttttgcaaa aaataaaata tccaatagcg
    10561 ctttaatcga aaaaagtctt ttgctttcta tcttttttcc tacaaataat aacaaatcag
    10621 aaaaagagaa aagtgacaag aaaattgctt atccccttct cttgctactt ttctctttct
    10681 atcaagttca aaaattattt tgtttcgtga tctgctaatg atgctccgac aaatgctttg
    10741 attaattttt gcgggcgatt tggacgagaa atcaattctg ggtggaattg gcatcccaca
    10801 aagaattttt tctctggcag ctctacgatt tctactaaac ggttatctgg tgagacacct
    10861 gaaaagacta gcccttgttc ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg
    10921 tgacggtgac gttcttgcac aacatcggct ccatcatatg cttcagccgt cttcgttcct
    10981 tttttgattt tacatgggta taatccaagg cgtaaagttc cacccaaatt ctcgatgttt
    11041 tcctgatcag ccattaaatc aataatattg tttgctgtat ctggattcgt ttcagctgat
    11101 gacgcatctt ctaatccaac gacgttacgt gcaaattcga cacaagccat ctgcatacca
    11161 agacaaatac ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc
    11221 ccttctactc cgcgatcacc aaatccacca ggaaccaaga ttccatctgc gtctttcaaa
    11281 cgttcttcta cattttctct cgttaattct tgtgctttga tccaatcgat ctcgatgtct
    11341 gaattatagg cgaaaccaga atgtttcaat gcttcaacta ctgagatata tgcatctggt
    11401 aactcgacat atttaccgac aagcgcaatc cgtacttttt tcttcaagtt caagacacgt
    11461 tcttctaatt ctttccattc tgtcatgtct gcttctggtg tatctagttt taaatggtcg
    11521 caaacaattt gatccattcc ttgtgcttgt aaaactaatg ggatggagta aagtgtatct
    11581 acatctggcg actcgataac tgcttctggt tcgacatcac aaaattgtgc caatttgttt
    11641 ttcacgcttt gagaaacagg ttgctctgta cgtaccacca aaatattcgg ttggatacct
    11701 aaaccacgta gctcttttac actgtgctgc gttggtttag ttttcatttc tccagcagct
    11761 ttcaagtaag gaatcaatgt tgtatggata tacataacat tatctgtacc gacatctgct
    11821 ttcatttgac gaagtgcttc aaggaatggc aaagattcta tatcccctac tgttccccct
    11881 acttctgtaa tgatcacatc tgaatcagtc attttagctg ctcgcatgat tttttctttg
    11941 atctcatttg taatatgagg aataacttga acagttgctc ccaagtattc gcccttacgt
    12001 tctttacgca aaacttcaga atagattttt cctgtcgtca cgttggagta tttgttcaaa
    12061 ttgatgtcaa taaaacgttc gtagtggccc agatccaaat ctgtttcagc accatcatct
    12121 gttacgaata cttctccgtg ctgatatggg ctcatcgtcc ccggatccac gttgatgtat
    12181 ggatcaaatt tttggatagt tacttttaag ccgcggtttt tcaataagcg tcctaaagaa
    12241 gctgcaacaa tcccttttcc gattgatgaa acgacaccgc cggtcacaaa aatatatttt
    12301 gtcataagtt caaaaactcc tttttaattt atgtgcagga aaagtctcag acaataaaca
    12361 aagctcccta ttcttcaata agaataggga gccgaaattt ctgtaaactt ctgaccctct
    12421 aaaagggtgc ccaaggtgta taatacaagc tgactaaggg aaagtcaagt actcaaatga
    12481 atactatccg cttacaattt tttctttttc gatatccttt gagaaattat ttcttaagtc
    12541 ctttatgagt gcaaaaaaaa tttaacccac ataataagct tgatataaac atagctaacg
    12601 ttaaacctaa gcaaatcaga aaatcgttac tgaaaaattt taagtgaaat aatagataaa
    12661 aaaataaaac aaacattatg atagataata tcccacctaa caaagaacta ctttttccta
    12721 cttgcagcaa cagtttatat agcttatcgt agaaacaatc tttttctttc acccttattt
    12781 acctccttta cgtaaaaaac gtagtccaca gttctcggat ttttggcata tcttttctat
    12841 taaataatat caaaaacaaa acaattccac taattttttc aaagatgacc tttttttcaa
    12901 caaaagtgcc ttgtagggat tcattgaagg taacgaatca cataagtaaa aaataaggag
    12961 atgcgacaaa agtcgtgcct ccttatttct aaataaacgg tgttctcctg ccaactcctc
    13021 ggtcacaagt cacaatatcc atcaaacatg agaagctgtt tttgaaatta tttcttgtga
    13081 ttcggagtta aatggtaggc tcacaacctc tggataaacg gtattcaaaa gccagtttct
    13141 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa
    13201 tactcgaaac taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat
    13261 ctgcggtaat aagttcagcc aattcaaaaa caagaaaagc tgtttttgct tggctattct
    13321 tattacttgt tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
    13381 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc cgttagtttc
    13441 tctaatactc aggtcaaaac gctgatttca cagcctctaa ttcacggtat tcctgtttta
    13501 ataaagttct agatattgtt ctctttccca ctcggaaaca gtttgtctga atgccgccca
    13561 ttcgagacgt tttgcttcaa caaaatttga gaagatatgt tgtcctaatg cgtcaatcat
    13621 tacattatcc ttacgcaatt ctttgatagc attatggata gttgaaggta agtcctggat
    13681 atgtgcagct tctctttctt cctcatccat tacataaatg ttacggtcaa cagctgctgg
    13741 cggtgtgatc tgattacgga taccgtctag tcctgcttgc aacaatactg ccattgccaa
    13801 gtatgggttt gcagatggat caactgaccg caattccaaa cgtgtggata aaccgcgaga
    13861 ttctggtaca cgtactaatg gcgaacgatt acggccactc catgcgacat aaacaggcgc
    13921 ttcataacct ggaacgagac gtttataaga attgactgtt ggattgcaaa cggctgtgta
    13981 ggcacgagca tgtttcagca aaccacctaa gaaatgataa gctgtttcac ttagttccaa
    14041 ttcaccgtct ttatcataga aaacatttcc ttcatcattg aacaaagaca tattgcaatg
    14101 cataccagaa ccattgatcc caaataacgg tttcggcatg aaagtcgcgt gaagtccatg
    14161 cttgcgtgca atcgttttga caacgagctt gaaggtttgg atattatcac aagcatctac
    14221 cacatctgca tatttaaagt caatttcatg ctgtcctggc gctacttcat gatgtgaagc
    14281 ttccacttca aagcctagac tttctaattc caacacgatg tcacggcggc aattttcacc
    14341 taaatctgtt ggtgcaaaat caaaataacc acctttatca ttcaaggtcg ttgtgatccc
    14401 accgtcttcg tctaatttaa ataagaagaa ttctggttca ggtcctaaat tgaaagaggt
    14461 aaaaccaagg tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc
    14521 aaatggtgtc ccatccggat tatagatatc acaaattaaa cgagcaactt ttccatgagc
    14581 actttcccac gggaagatca tccaagtgga gagatctgga tacaaataca tatcgctttc
    14641 ttcaattcga acaaatcctt caatcgaaga accgtcaaac atcattttat tttctaatac
    14701 tttgtcgatc tggctcactg gaacttctac atttttgatc gtacctaaga tgtctgtgaa
    14761 catcaatcgt aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt cttcacctgt
    14821 tatctgtttc tttaccatgg tatccctctt ccttttttaa tttattcagt caaagaattg
    14881 tctttgcaat ccttgcgact gataatgacc tgttttgctt aacccacctt gcgccaaaat
    14941 ctcatcacga aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc
    15001 ttgttttttc gcctgtgcca tttcgtatac acgtttgatc ccagccatat tcaatccctc
    15061 tgaaagatag tctttaatct caagcaacat atcaatatca ttcagcgaat acatacgtcg
    15121 gtttccttcg cttcgttccg gatgaactaa atcttgttct tcataatagc gaatttgccg
    15181 agcagacaaa tcagtcagct tcataactgt accaattgga aatacggaca tagatcggcg
    15241 caattccttt tctcccatca gacgtcccct ttcgttttat aagttggcta aagtgtacca
    15301 attaaaaaaa atattgtcaa ctgcatatgt taggttttct aacatgagat ctctattttt
    15361 tataaaataa caaaaaaata agaacacaaa aaaggtatct agttgatttt cgtaccagat
    15421 accttttatt ttttattata ctgtactatc ctttattagt gtttttatt
//
LOCUS       DADVXN010000069        15416 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.071, whole genome shotgun sequence.
ACCESSION   DADVXN010000069 DADVXN010000000
VERSION     DADVXN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.071"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            82..2142
                     /locus_tag="KB942_001866"
     CDS             82..2142
                     /locus_tag="KB942_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBB6852288.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2225..2794
                     /locus_tag="KB942_001867"
     CDS             2225..2794
                     /locus_tag="KB942_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852289.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2969..3775)
                     /locus_tag="KB942_001868"
     CDS             complement(2969..3775)
                     /locus_tag="KB942_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852290.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3913..5427
                     /locus_tag="KB942_001869"
     CDS             3913..5427
                     /locus_tag="KB942_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBB6852291.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5830..6813)
                     /locus_tag="KB942_001870"
     CDS             complement(5830..6813)
                     /locus_tag="KB942_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBB6852292.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6818..7465)
                     /locus_tag="KB942_001871"
     CDS             complement(6818..7465)
                     /locus_tag="KB942_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6852293.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7550..8182)
                     /locus_tag="KB942_001872"
     CDS             complement(7550..8182)
                     /locus_tag="KB942_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6852294.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="KB942_001873"
     CDS             complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="KB942_001873"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBB6852295.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8746..9483)
                     /locus_tag="KB942_001874"
     CDS             complement(8746..9483)
                     /locus_tag="KB942_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBB6852296.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9480..10469)
                     /locus_tag="KB942_001875"
     CDS             complement(9480..10469)
                     /locus_tag="KB942_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6852297.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10727..11269
                     /locus_tag="KB942_001876"
     CDS             10727..11269
                     /locus_tag="KB942_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852298.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11286..12371
                     /locus_tag="KB942_001877"
     CDS             11286..12371
                     /locus_tag="KB942_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852299.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12383..13189
                     /locus_tag="KB942_001878"
     CDS             12383..13189
                     /locus_tag="KB942_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852300.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13190..14017
                     /locus_tag="KB942_001879"
     CDS             13190..14017
                     /locus_tag="KB942_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852301.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14014..15087
                     /locus_tag="KB942_001880"
     CDS             14014..15087
                     /locus_tag="KB942_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852302.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4957 a         2781 c         3141 g         4537 t
ORIGIN      
        1 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
       61 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      121 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      181 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      241 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      301 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      361 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      421 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      481 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      541 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      601 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      661 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      721 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      781 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      841 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      901 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
      961 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1021 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1081 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1141 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1201 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1261 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1321 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1381 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1441 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1501 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1561 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1621 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1681 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1741 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1801 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1861 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1921 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     1981 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2041 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2101 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2161 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2221 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2281 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2341 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2401 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2461 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2521 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaatcagatt accaaggaaa
     2581 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2641 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2701 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2761 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2821 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2881 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     2941 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3001 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3061 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3121 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3181 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3241 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3301 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3361 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3421 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3481 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3541 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3601 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3661 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3721 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3781 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3841 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3901 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     3961 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4021 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4081 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4141 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4201 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4261 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4321 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4381 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4441 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4501 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4561 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4621 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4681 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4741 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4801 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4861 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4921 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     4981 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5041 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5101 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5161 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5221 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5281 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5341 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5401 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5461 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5521 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
     5581 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa
     5641 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     5701 gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     5761 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc
     5821 agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat
     5881 atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat
     5941 cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt
     6001 atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa
     6061 tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg
     6121 tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa
     6181 aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt
     6241 tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga
     6301 tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt
     6361 tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag
     6421 ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata
     6481 gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata
     6541 accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact
     6601 tttattctca actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa
     6661 agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat
     6721 cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac
     6781 agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt
     6841 ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat
     6901 ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg
     6961 atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg
     7021 tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat
     7081 gtttagtaat ataataatct gtcatggact caggccattt tgctggttca tctgcataaa
     7141 cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga
     7201 ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca
     7261 atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag
     7321 agacatacga aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg
     7381 caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt
     7441 ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc
     7501 aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa
     7561 gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg
     7621 cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag
     7681 catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat
     7741 tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg
     7801 aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc
     7861 caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc
     7921 cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac
     7981 ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa
     8041 cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga
     8101 taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg
     8161 taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag
     8221 tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc
     8281 cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt
     8341 ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca
     8401 attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc
     8461 cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt
     8521 gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat
     8581 ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt
     8641 tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc
     8701 ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc
     8761 cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac
     8821 tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc
     8881 atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag
     8941 agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca
     9001 ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt
     9061 gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc
     9121 cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact
     9181 aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt
     9241 tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt
     9301 tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt
     9361 atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt
     9421 tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt
     9481 catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta
     9541 gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc
     9601 tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa
     9661 tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg
     9721 attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga
     9781 ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa
     9841 gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt
     9901 tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt
     9961 ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact
    10021 tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac
    10081 cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac
    10141 cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca
    10201 aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca
    10261 gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg
    10321 ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca
    10381 ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt
    10441 attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt
    10501 atcataccat aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag
    10561 gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca
    10621 aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta
    10681 gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga
    10741 gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac
    10801 agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga
    10861 aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga
    10921 agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga
    10981 aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt
    11041 cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac
    11101 gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc
    11161 aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct
    11221 caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta
    11281 aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa
    11341 cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc
    11401 cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag
    11461 aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag
    11521 taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg
    11581 cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg
    11641 ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg
    11701 gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat
    11761 tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc
    11821 gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag
    11881 cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta
    11941 cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga
    12001 gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat
    12061 ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag
    12121 aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc
    12181 tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg
    12241 tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag
    12301 atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct
    12361 acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat
    12421 tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt
    12481 tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc
    12541 gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat
    12601 catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat
    12661 gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat
    12721 tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca
    12781 gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat
    12841 gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag
    12901 agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa
    12961 tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac
    13021 tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt
    13081 ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt
    13141 catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt
    13201 taaatggtcg tatctttatt tagggatcgt ctttttattc ctatatgcac cgatcttcta
    13261 tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg
    13321 ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt
    13381 catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg
    13441 aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt
    13501 attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg
    13561 attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt
    13621 cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag
    13681 agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc
    13741 aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac
    13801 atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag
    13861 acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat
    13921 attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa
    13981 cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta
    14041 ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt
    14101 ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt
    14161 ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct
    14221 aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca
    14281 agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg
    14341 aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt tgatccagat
    14401 aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa cgataaattc
    14461 tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt
    14521 ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat
    14581 tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta
    14641 acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa
    14701 gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat
    14761 ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca
    14821 aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa
    14881 aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg
    14941 ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc
    15001 catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc
    15061 ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa
    15121 cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa
    15181 gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag
    15241 agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt
    15301 gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt
    15361 aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat gaaaaa
//
LOCUS       DADVXN010000070        15226 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.072, whole genome shotgun sequence.
ACCESSION   DADVXN010000070 DADVXN010000000
VERSION     DADVXN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.072"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            278..787
                     /locus_tag="KB942_001881"
     CDS             278..787
                     /locus_tag="KB942_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6852303.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="KB942_001882"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="KB942_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6852304.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="KB942_001883"
     CDS             2322..3302
                     /locus_tag="KB942_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6852305.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="KB942_001884"
     CDS             3424..4842
                     /locus_tag="KB942_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBB6852306.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="KB942_001885"
     CDS             4913..6382
                     /locus_tag="KB942_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6852307.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="KB942_001886"
     CDS             6392..6859
                     /locus_tag="KB942_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6852308.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="KB942_001887"
     CDS             6895..7767
                     /locus_tag="KB942_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6852309.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="KB942_001888"
     CDS             7848..10382
                     /locus_tag="KB942_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6852310.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="KB942_001889"
     CDS             10538..11311
                     /locus_tag="KB942_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852311.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="KB942_001890"
     CDS             11304..12095
                     /locus_tag="KB942_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6852312.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="KB942_001891"
     CDS             12105..12596
                     /locus_tag="KB942_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852313.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="KB942_001892"
     CDS             12614..13024
                     /locus_tag="KB942_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852314.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="KB942_001893"
     CDS             13028..14428
                     /locus_tag="KB942_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBB6852315.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="KB942_001894"
     CDS             14451..14825
                     /locus_tag="KB942_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6852316.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2133 c         3045 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactc
//
LOCUS       DADVXN010000071        14991 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.073, whole genome shotgun sequence.
ACCESSION   DADVXN010000071 DADVXN010000000
VERSION     DADVXN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.073"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            34..1164
                     /gene="dnaN"
                     /locus_tag="KB942_001895"
     CDS             34..1164
                     /gene="dnaN"
                     /locus_tag="KB942_001895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBB6852317.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1393..1635
                     /gene="yaaA"
                     /locus_tag="KB942_001896"
     CDS             1393..1635
                     /gene="yaaA"
                     /locus_tag="KB942_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBB6852318.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            1622..2746
                     /gene="recF"
                     /locus_tag="KB942_001897"
     CDS             1622..2746
                     /gene="recF"
                     /locus_tag="KB942_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBB6852319.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            2743..4689
                     /gene="gyrB"
                     /locus_tag="KB942_001898"
     CDS             2743..4689
                     /gene="gyrB"
                     /locus_tag="KB942_001898"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBB6852320.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            4709..7180
                     /gene="gyrA"
                     /locus_tag="KB942_001899"
     CDS             4709..7180
                     /gene="gyrA"
                     /locus_tag="KB942_001899"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBB6852321.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            7416..7715
                     /locus_tag="KB942_001900"
     CDS             7416..7715
                     /locus_tag="KB942_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBB6852322.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            7765..8277
                     /gene="ssb"
                     /locus_tag="KB942_001901"
     CDS             7765..8277
                     /gene="ssb"
                     /locus_tag="KB942_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6852323.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            8303..8539
                     /locus_tag="KB942_001902"
     CDS             8303..8539
                     /locus_tag="KB942_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBB6852324.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            8682..10655
                     /locus_tag="KB942_001903"
     CDS             8682..10655
                     /locus_tag="KB942_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBB6852325.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            10661..11113
                     /gene="rplI"
                     /locus_tag="KB942_001904"
     CDS             10661..11113
                     /gene="rplI"
                     /locus_tag="KB942_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBB6852326.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            11387..12754
                     /gene="dnaB"
                     /locus_tag="KB942_001905"
     CDS             11387..12754
                     /gene="dnaB"
                     /locus_tag="KB942_001905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBB6852327.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            13534..14826
                     /locus_tag="KB942_001906"
     CDS             13534..14826
                     /locus_tag="KB942_001906"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBB6852328.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4996 a         2699 c         3244 g         4052 t
ORIGIN      
        1 aataaataaa caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc
       61 tttatgcagg aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt
      121 ttgacaggtg taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct
      181 gatatatcaa ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa
      241 tctactggtt ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct
      301 gaagaaacat ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa
      361 gcgaatttta tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa
      421 agccataacc agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt
      481 tttgctgtct cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct
      541 gataattctt tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca
      601 gtagaacaag cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta
      661 tctcgctcat taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg
      721 ttgttcaaaa cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat
      781 accaatcgtt tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt
      841 ttagcggcga tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt
      901 ttatctattc gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta
      961 gaagaaagct tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg
     1021 gattatatga aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc
     1081 tctgctattc gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg
     1141 attactcctg tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa
     1201 agaaatagaa aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg
     1261 tttatttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg
     1321 ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat
     1381 gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa
     1441 atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa
     1501 aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc
     1561 atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat
     1621 aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga
     1681 attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt
     1741 ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt
     1801 gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac
     1861 tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga
     1921 acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga
     1981 tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg
     2041 ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag
     2101 aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat
     2161 tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt
     2221 caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca
     2281 gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat
     2341 ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac
     2401 aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca
     2461 gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat
     2521 cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag
     2581 cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac
     2641 gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat
     2701 tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt
     2761 ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa
     2821 gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac
     2881 cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa
     2941 atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata
     3001 cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta
     3061 catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta
     3121 ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga
     3181 aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa
     3241 caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag
     3301 acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac
     3361 cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac
     3421 cattatgacg gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt
     3481 tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg
     3541 caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat
     3601 gaaggtggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat
     3661 gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga
     3721 gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca
     3781 aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat
     3841 ttcatgaaat tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta
     3901 ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt
     3961 gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa
     4021 aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt
     4081 aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca
     4141 agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca
     4201 gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact
     4261 gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac
     4321 atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta
     4381 aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa
     4441 caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt
     4501 ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg
     4561 gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg
     4621 gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta
     4681 gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga
     4741 tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt
     4801 cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata
     4861 cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt
     4921 cggagatgtc atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt
     4981 acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg
     5041 ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat
     5101 cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga
     5161 tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg
     5221 aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt
     5281 agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga
     5341 agtattacct ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg
     5401 aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga
     5461 gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc
     5521 aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga
     5581 tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc
     5641 cagcgcatcc gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg
     5701 attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct
     5761 tgaaaactat atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga
     5821 aaaagcagaa gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga
     5881 tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat
     5941 cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg
     6001 attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat
     6061 tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga
     6121 attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga
     6181 ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac
     6241 aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg
     6301 acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg
     6361 ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg
     6421 atacgaaatt ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg
     6481 tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg
     6541 ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt
     6601 tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt
     6661 caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc
     6721 tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg
     6781 gatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga
     6841 agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt
     6901 gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact
     6961 agctggattg accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt
     7021 cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg
     7081 tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc
     7141 tgcagaacaa acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta
     7201 agctagcaag aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt
     7261 gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt
     7321 gtataattag agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac
     7381 ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca
     7441 tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg
     7501 atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc
     7561 gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt
     7621 ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata
     7681 tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga
     7741 aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg
     7801 aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct
     7861 gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg
     7921 atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt
     7981 gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg
     8041 acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc
     8101 caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat
     8161 aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct
     8221 gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg
     8281 aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa
     8341 agtagactac attgctgcta accatattga atatatcgat tataaagata tcgaattact
     8401 aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc
     8461 taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc
     8521 tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta
     8581 ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa
     8641 gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc
     8701 atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct
     8761 taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg
     8821 ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc
     8881 aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag
     8941 tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa
     9001 tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag
     9061 aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag
     9121 taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga
     9181 cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata
     9241 aattagatga caaagagatt tcttatttga actctttcgt cactacacta gtttctgact
     9301 ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag
     9361 cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc
     9421 gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct
     9481 caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc
     9541 gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg
     9601 gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg
     9661 ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg
     9721 atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa
     9781 aagcttacat cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag
     9841 aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc
     9901 tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct
     9961 ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg
    10021 aagagtttcc aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag
    10081 agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg
    10141 aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa
    10201 ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag
    10261 tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg
    10321 gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata
    10381 atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag
    10441 ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca
    10501 cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag
    10561 ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca
    10621 aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca
    10681 agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca
    10741 aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt
    10801 agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa
    10861 gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga
    10921 agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca
    10981 atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg
    11041 ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt
    11101 cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa
    11161 aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa
    11221 tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg
    11281 agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc
    11341 tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg
    11401 gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt
    11461 tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg
    11521 tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat
    11581 tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg
    11641 tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta
    11701 tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat
    11761 cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg
    11821 cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt
    11881 actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg
    11941 tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact
    12001 aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca
    12061 aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga
    12121 atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac
    12181 aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg
    12241 agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg
    12301 tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt
    12361 gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact
    12421 gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg
    12481 agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat
    12541 tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg
    12601 cggagatgac gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga
    12661 gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa
    12721 gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat
    12781 cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat
    12841 ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata
    12901 aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac
    12961 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc
    13021 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag
    13081 aagcagtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct
    13141 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata
    13201 tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    13261 ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa
    13321 atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac
    13381 aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa
    13441 aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt
    13501 tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg
    13561 caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg
    13621 attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact
    13681 tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga
    13741 aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa
    13801 ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat
    13861 atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt
    13921 aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac
    13981 ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt
    14041 caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat
    14101 tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat
    14161 gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc
    14221 gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc
    14281 ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg
    14341 tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc
    14401 cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt
    14461 gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac
    14521 tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat
    14581 ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc
    14641 tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg
    14701 ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca
    14761 acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa
    14821 atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt
    14881 tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg
    14941 ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa a
//
LOCUS       DADVXN010000072        14820 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.074, whole genome shotgun sequence.
ACCESSION   DADVXN010000072 DADVXN010000000
VERSION     DADVXN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.074"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(296..1024)
                     /locus_tag="KB942_001907"
     CDS             complement(296..1024)
                     /locus_tag="KB942_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBB6852329.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="KB942_001908"
     CDS             complement(1002..2576)
                     /locus_tag="KB942_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6852330.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="KB942_001909"
     CDS             2805..3743
                     /locus_tag="KB942_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6852331.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="KB942_001910"
     CDS             3755..4666
                     /locus_tag="KB942_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6852332.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="KB942_001911"
     CDS             4690..6162
                     /locus_tag="KB942_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6852333.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="KB942_001912"
     CDS             6213..6824
                     /locus_tag="KB942_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6852334.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="KB942_001913"
     CDS             6854..8767
                     /locus_tag="KB942_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HBB6852335.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="KB942_001914"
     CDS             8895..11876
                     /locus_tag="KB942_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HBB6852336.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="KB942_001915"
     CDS             11873..13534
                     /locus_tag="KB942_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HBB6852337.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="KB942_001916"
     CDS             13574..14506
                     /locus_tag="KB942_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBB6852338.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DADVXN010000073        14723 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.075, whole genome shotgun sequence.
ACCESSION   DADVXN010000073 DADVXN010000000
VERSION     DADVXN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.075"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(195..761)
                     /locus_tag="KB942_001917"
     CDS             complement(195..761)
                     /locus_tag="KB942_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6852339.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(761..1717)
                     /locus_tag="KB942_001918"
     CDS             complement(761..1717)
                     /locus_tag="KB942_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBB6852340.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1717..2373)
                     /locus_tag="KB942_001919"
     CDS             complement(1717..2373)
                     /locus_tag="KB942_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6852341.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2743..4761
                     /locus_tag="KB942_001920"
     CDS             2743..4761
                     /locus_tag="KB942_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6852342.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4831..5037
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5188..7602
                     /locus_tag="KB942_001921"
     CDS             5188..7602
                     /locus_tag="KB942_001921"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBB6852343.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7950..9503
                     /locus_tag="KB942_001922"
     CDS             7950..9503
                     /locus_tag="KB942_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6852344.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9884..11668)
                     /locus_tag="KB942_001923"
     CDS             complement(9884..11668)
                     /locus_tag="KB942_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852345.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11683..13404)
                     /locus_tag="KB942_001924"
     CDS             complement(11683..13404)
                     /locus_tag="KB942_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852346.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13504..14265
                     /locus_tag="KB942_001925"
     CDS             13504..14265
                     /locus_tag="KB942_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6852347.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4788 a         2718 c         2925 g         4292 t
ORIGIN      
        1 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
       61 tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt
      121 atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt
      181 tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt
      241 tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa
      301 gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc
      361 aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct
      421 ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct
      481 ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat
      541 gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt
      601 tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc
      661 gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct
      721 tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc
      781 gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca
      841 agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc
      901 ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat
      961 tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc
     1021 tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa
     1081 tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca
     1141 aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt
     1201 gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa
     1261 gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg
     1321 gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt
     1381 gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat
     1441 aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga
     1501 attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc
     1561 aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat
     1621 cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat
     1681 gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga
     1741 gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag
     1801 actgagtaag taaccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct
     1861 tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct
     1921 tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc
     1981 tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg
     2041 ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc
     2101 agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca
     2161 agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct
     2221 gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag
     2281 ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct
     2341 accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt
     2401 attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc
     2461 ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg
     2521 gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa
     2581 gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg gtaaattaca
     2641 atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt aagagaagtt
     2701 ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa cattgcaaga
     2761 aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg aggggcagca
     2821 atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca gccggttcct
     2881 gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc ggcatttatc
     2941 acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata tgcatcagtg
     3001 atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc tcaagcacct
     3061 tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta catggatacg
     3121 cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca atatccttct
     3181 tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt tggcaacggt
     3241 tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc gacagcttgt
     3301 ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa tatcatcgca
     3361 acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg tggagcgact
     3421 acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa ttctggtaat
     3481 acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga ttctgtttgg
     3541 ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa caacatccaa
     3601 aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga aaatggctct
     3661 tcgacgaata attccggtaa caataccaat tcttcaggaa atgctggtac atcaaacgga
     3721 ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg gaagatctca
     3781 aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa gaacaatttc
     3841 gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc tggagcaaat
     3901 actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag ttcttctaat
     3961 acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag cgtctcaaat
     4021 aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa taactttatc
     4081 tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac gtcaggtaat
     4141 acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag cagttcgtct
     4201 caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa taaaaacggc
     4261 atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat ttatccagga
     4321 cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc aactacttct
     4381 gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc cactggtaat
     4441 acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa acatcatatc
     4501 acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta tccaggacag
     4561 caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt tactggttcg
     4621 aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag tcatggaatt
     4681 acaatggctc aactaatcga atggaacaat attaagaaca actttattta tccaggtcaa
     4741 acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa ttccacaaaa
     4801 atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag tagtaaaaag
     4861 actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt tgaactcgcc
     4921 tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc gttacaacgt
     4981 tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt cctataaaaa
     5041 gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa acaaagcaaa
     5101 aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat agaaacaagt
     5161 gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat tgagaaaaaa
     5221 tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc tgaaaagcca
     5281 aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca tgtagggcat
     5341 cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca aggatacaac
     5401 gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata tgcgctagat
     5461 acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg acgccaaatc
     5521 aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga tccggagtat
     5581 tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc ttatgaagca
     5641 gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga agaagtgatc
     5701 gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg ccaatggatg
     5761 ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt tgattggcca
     5821 gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc aaatattgaa
     5881 tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc agatactcta
     5941 ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga aattacgacc
     6001 cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa atcagatcta
     6061 aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta tgcaatcaat
     6121 ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc ttcatacggc
     6181 acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt cgctcaaact
     6241 ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc tgcttacaca
     6301 gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca agaagcaatc
     6361 gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt cagctatcgc
     6421 ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc aatcatccat
     6481 tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg gttgcctaag
     6541 acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat cgaggagtgg
     6601 gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa tacaatgcca
     6661 caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa caaaaaagaa
     6721 ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat tggtggagca
     6781 gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt atacgatatc
     6841 ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat gatcttaggc
     6901 gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga tgatgtggtc
     6961 gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc attagatgct
     7021 tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga tcgtgtatgg
     7081 cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt gaatgatggc
     7141 aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta tgaaaatctt
     7201 catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta taaagtggac
     7261 gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat tgctcctcac
     7321 atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta cgcaccatgg
     7381 ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt ccaagtaaac
     7441 ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga gctagaaaaa
     7501 gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt cagaaaagtt
     7561 attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct ttgtcaataa
     7621 ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc tgtctcagat
     7681 tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt tcatgttctc
     7741 aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg taatcatcaa
     7801 atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat tgagagtaca
     7861 taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa
     7921 tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat
     7981 tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca
     8041 agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat
     8101 atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt
     8161 atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac
     8221 ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag
     8281 cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga
     8341 agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat
     8401 cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat
     8461 tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg
     8521 gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg
     8581 gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt
     8641 ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt
     8701 tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg
     8761 atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc
     8821 cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg
     8881 aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt
     8941 cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt
     9001 ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg
     9061 atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca
     9121 ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag
     9181 tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt
     9241 tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact
     9301 atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga
     9361 atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt
     9421 ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat
     9481 cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt
     9541 cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt
     9601 cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga
     9661 tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt
     9721 gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat
     9781 tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca
     9841 tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt
     9901 gactattata aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt
     9961 tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca
    10021 aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa
    10081 tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag
    10141 gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc
    10201 cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac
    10261 attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg
    10321 cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag
    10381 aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc
    10441 ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag
    10501 ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca
    10561 atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt
    10621 gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa
    10681 atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag
    10741 aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca
    10801 caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat
    10861 taatgaaacg aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt
    10921 tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg
    10981 ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa acacccagtt
    11041 catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg
    11101 aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca
    11161 tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata
    11221 acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat
    11281 gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc
    11341 tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc
    11401 tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat
    11461 cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc
    11521 cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa
    11581 acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac
    11641 gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt
    11701 tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac
    11761 gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg
    11821 agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc
    11881 atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt
    11941 gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc
    12001 ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc
    12061 gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt
    12121 gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg
    12181 gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt
    12241 agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc
    12301 aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc
    12361 agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa
    12421 ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat
    12481 ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc
    12541 gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat
    12601 ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat
    12661 tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat
    12721 ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc
    12781 aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc
    12841 catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat
    12901 ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa
    12961 catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa
    13021 aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga
    13081 actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg
    13141 cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc
    13201 gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc
    13261 ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt
    13321 accggcgatt tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg
    13381 taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact tttcgattct
    13441 ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa
    13501 aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca
    13561 acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat
    13621 tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa
    13681 ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat
    13741 gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa
    13801 caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa
    13861 gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt
    13921 gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct
    13981 tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact
    14041 caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc
    14101 gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa
    14161 ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat
    14221 atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa
    14281 gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg
    14341 gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata
    14401 tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt
    14461 aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac
    14521 taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag aatgataaat
    14581 taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt attacaatat
    14641 gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat caaatagtcc
    14701 ctactgtgac tttcctcagc tat
//
LOCUS       DADVXN010000074        14338 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.076, whole genome shotgun sequence.
ACCESSION   DADVXN010000074 DADVXN010000000
VERSION     DADVXN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.076"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            476..1339
                     /locus_tag="KB942_001926"
     CDS             476..1339
                     /locus_tag="KB942_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6852348.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="KB942_001927"
     CDS             complement(1468..3090)
                     /locus_tag="KB942_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6852349.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="KB942_001928"
     CDS             3339..3860
                     /locus_tag="KB942_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6852350.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KB942_001929"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KB942_001929"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBB6852351.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="KB942_001930"
     CDS             5941..6759
                     /locus_tag="KB942_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBB6852352.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="KB942_001931"
     CDS             complement(6862..7059)
                     /locus_tag="KB942_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852353.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="KB942_001932"
     CDS             7228..7806
                     /locus_tag="KB942_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6852354.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="KB942_001933"
     CDS             7901..8038
                     /locus_tag="KB942_001933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852355.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="KB942_001934"
     CDS             complement(8851..9354)
                     /locus_tag="KB942_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6852356.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="KB942_001935"
     CDS             9713..10738
                     /locus_tag="KB942_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6852357.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="KB942_001936"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="KB942_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            12415..12531
                     /locus_tag="KB942_001937"
     CDS             12415..12531
                     /locus_tag="KB942_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6852358.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="KB942_001938"
     CDS             13459..13797
                     /locus_tag="KB942_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852359.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="KB942_001939"
     CDS             13891..14121
                     /locus_tag="KB942_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852360.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4550 a         2390 c         2734 g         4664 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattcta
//
LOCUS       DADVXN010000075        14291 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.077, whole genome shotgun sequence.
ACCESSION   DADVXN010000075 DADVXN010000000
VERSION     DADVXN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.077"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(51..1598)
                     /locus_tag="KB942_001940"
     CDS             complement(51..1598)
                     /locus_tag="KB942_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBB6852361.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB942_001941"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB942_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB6852362.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="KB942_001942"
     CDS             complement(2043..2237)
                     /locus_tag="KB942_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852363.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="KB942_001943"
     CDS             complement(2463..3665)
                     /locus_tag="KB942_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6852364.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="KB942_001944"
     CDS             complement(3668..3862)
                     /locus_tag="KB942_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6852365.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="KB942_001945"
     CDS             complement(3909..4757)
                     /locus_tag="KB942_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HBB6852366.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="KB942_001946"
     CDS             complement(4962..5312)
                     /locus_tag="KB942_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852367.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5907..6353
                     /locus_tag="KB942_001947"
     CDS             5907..6353
                     /locus_tag="KB942_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6852368.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            6977..7969
                     /locus_tag="KB942_001948"
     CDS             6977..7969
                     /locus_tag="KB942_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6852369.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7985..8869
                     /locus_tag="KB942_001949"
     CDS             7985..8869
                     /locus_tag="KB942_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6852370.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8918..10525
                     /locus_tag="KB942_001950"
     CDS             8918..10525
                     /locus_tag="KB942_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852371.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10526..12010
                     /locus_tag="KB942_001951"
     CDS             10526..12010
                     /locus_tag="KB942_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBB6852372.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12187..13179
                     /locus_tag="KB942_001952"
     CDS             12187..13179
                     /locus_tag="KB942_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6852373.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13231..14100
                     /locus_tag="KB942_001953"
     CDS             13231..14100
                     /locus_tag="KB942_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6852374.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         4371 a         2434 c         2890 g         4596 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtattata tacgagaact
     5401 tatacgtagt caagagattt tctaaaaaaa tatatacgat tttttatacg agtgttataa
     5461 tgaaaaaaat gataaggagg aatttgaata tgtttggttt agtacaacct aataaagtga
     5521 aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt acagaattag
     5581 ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc tatgcgttgg
     5641 ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc ggctggtttt
     5701 attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt cagaaaaaaa
     5761 tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat acgggcgagt
     5821 tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt atccaagaat
     5881 gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt acgaaagagg
     5941 tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa gaagctgtca
     6001 cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc aagtatggag
     6061 agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat gtgaaacaag
     6121 acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt ggtacagatg
     6181 aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag catttttcca
     6241 ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca gcgttaatca
     6301 aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag taaaataaga
     6361 aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat cagtaatcta
     6421 taaaggggtt gttgattata ctgtagaagt taagtactca agagattttt tgtacgtaaa
     6481 caaagcaaaa aagggtgcag tttcactaga aaatgtactc ttttttgtga tttttcttag
     6541 ttatagagtt tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga
     6601 catagcttct tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta
     6661 aatcacattt tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc
     6721 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
     6781 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
     6841 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
     6901 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
     6961 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
     7021 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
     7081 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
     7141 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
     7201 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
     7261 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
     7321 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
     7381 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
     7441 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
     7501 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
     7561 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
     7621 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
     7681 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     7741 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     7801 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     7861 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     7921 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     7981 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     8041 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     8101 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     8161 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     8221 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     8281 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     8341 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     8401 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     8461 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     8521 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     8581 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     8641 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     8701 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     8761 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     8821 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     8881 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     8941 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     9001 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     9061 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     9121 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     9181 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     9241 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     9301 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     9361 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     9421 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     9481 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     9541 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     9601 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     9661 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     9721 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     9781 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     9841 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     9901 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     9961 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
    10021 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
    10081 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
    10141 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
    10201 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
    10261 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
    10321 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
    10381 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
    10441 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
    10501 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
    10561 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
    10621 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
    10681 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
    10741 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
    10801 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
    10861 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
    10921 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
    10981 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
    11041 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
    11101 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
    11161 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
    11221 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
    11281 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
    11341 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
    11401 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
    11461 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
    11521 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
    11581 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
    11641 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
    11701 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
    11761 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
    11821 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
    11881 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
    11941 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
    12001 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
    12061 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
    12121 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
    12181 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
    12241 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
    12301 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
    12361 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
    12421 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
    12481 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
    12541 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
    12601 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
    12661 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
    12721 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
    12781 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
    12841 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
    12901 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
    12961 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
    13021 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
    13081 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
    13141 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
    13201 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
    13261 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
    13321 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
    13381 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
    13441 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
    13501 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
    13561 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
    13621 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
    13681 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
    13741 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
    13801 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
    13861 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
    13921 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
    13981 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
    14041 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
    14101 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
    14161 taaatagtct caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt
    14221 caaattaata taattacgaa atgatattga gtacaagaaa gttcttttta ccagatcttt
    14281 taaatcgaaa a
//
LOCUS       DADVXN010000076        13428 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.078, whole genome shotgun sequence.
ACCESSION   DADVXN010000076 DADVXN010000000
VERSION     DADVXN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.078"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(45..737)
                     /locus_tag="KB942_001954"
     CDS             complement(45..737)
                     /locus_tag="KB942_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6852375.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1174..1743)
                     /locus_tag="KB942_001955"
     CDS             complement(1174..1743)
                     /locus_tag="KB942_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6852376.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="KB942_001956"
     CDS             complement(1817..2140)
                     /locus_tag="KB942_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852377.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="KB942_001957"
     CDS             complement(2294..3106)
                     /locus_tag="KB942_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852378.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3459..4307
                     /locus_tag="KB942_001958"
     CDS             3459..4307
                     /locus_tag="KB942_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBB6852379.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4452..5393
                     /locus_tag="KB942_001959"
     CDS             4452..5393
                     /locus_tag="KB942_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBB6852380.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5383..7554
                     /locus_tag="KB942_001960"
                     /pseudo
     CDS             5383..7554
                     /locus_tag="KB942_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7666..9837)
                     /gene="copB"
                     /locus_tag="KB942_001961"
     CDS             complement(7666..9837)
                     /gene="copB"
                     /locus_tag="KB942_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBB6852381.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9857..12043)
                     /locus_tag="KB942_001962"
     CDS             complement(9857..12043)
                     /locus_tag="KB942_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBB6852382.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12043..12252)
                     /gene="copZ"
                     /locus_tag="KB942_001963"
     CDS             complement(12043..12252)
                     /gene="copZ"
                     /locus_tag="KB942_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBB6852383.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12265..12705)
                     /locus_tag="KB942_001964"
     CDS             complement(12265..12705)
                     /locus_tag="KB942_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBB6852384.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12779..13318)
                     /locus_tag="KB942_001965"
     CDS             complement(12779..13318)
                     /locus_tag="KB942_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6852385.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3771 a         2813 c         2389 g         4455 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaacat aataagatca ctttgcttgt
     3301 aggatgtatt cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga
     3361 aaatggaaga aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag
     3421 aaaaagcggt catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag
     3481 gtggtcatgt cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac
     3541 tagcaaaagc tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta
     3601 gtgatattct tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg
     3661 gaccgatctt taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc
     3721 agtcagcact tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc
     3781 ctctagacaa tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat
     3841 atcctttgcc ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt
     3901 cttttgatga cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa
     3961 atgaagtatt gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg
     4021 tcagtcccga agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg
     4081 ccatccagct tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt
     4141 atgctttaat ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt
     4201 acgtgttttc ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac
     4261 aaatcattca tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca
     4321 atggaaaaaa tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt
     4381 gtgctttttg ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact
     4441 ctttatgtat catggagctt gatttcatta agcagctttc taaagaaact ttcggttact
     4501 tgtacattgc agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca
     4561 aaaaagagaa ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt
     4621 gcagaaaaaa acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt
     4681 ggcagccaaa agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata
     4741 gtctaggact tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc
     4801 ctgctttttg taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc
     4861 tgaacgcgta ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt
     4921 ctttcaaccg aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat
     4981 acgattatca agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa
     5041 aatttgaacc tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc
     5101 cggaacttct gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt
     5161 ttgcttcttt ggaagctggc gatgagaaag cttttttcgc acacttgaaa aaatatgcaa
     5221 aaaaacaaat cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa
     5281 cattgtcaaa agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc
     5341 aagaaaaaga caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa
     5401 aaatggctgc gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta
     5461 gtagctaatc aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt
     5521 attgtctgct gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata
     5581 ggttgtttgt ttttatattt tccttcgaag acattttcca tattatggat cttacagttt
     5641 ttttcgtcag tcacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca
     5701 gaaaagacag ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg
     5761 gattacgact attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc
     5821 gtttttcgga aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg
     5881 tatctggcag caaggcaatt tttctccatc cggttgttgt cgggaaacac tcgattccag
     5941 tcattgacta cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat
     6001 cttccgaaag tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat
     6061 gatgaagggc tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc
     6121 ttcagtgaga atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt
     6181 acagcgaacc aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt
     6241 tctggatgga tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac
     6301 atcacgagct accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa
     6361 caagttttct taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt
     6421 ggacaaacgc tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg
     6481 acacgttctg caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc
     6541 agtcagttaa aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt
     6601 ttgcctaaac gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt
     6661 cgtaaagtcg aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa
     6721 acggataccg ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca
     6781 aaagtcggct attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt
     6841 atcccagctc gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa
     6901 gggaatgagg aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt
     6961 cctggatttg gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca
     7021 caaccagttg ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc
     7081 gaaaatatgg cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca
     7141 acactcacac cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg
     7201 ttgttctttc tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt
     7261 catttttttg cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca
     7321 ttggtaccgc gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa
     7381 ttcaagctag atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga
     7441 cgacaagcag atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata
     7501 agaaaaatgg tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa
     7561 atttctggag aatctacttc tggaatgccg agaaccagct gattgagtat cgattactcg
     7621 aaaaaaaggt tgtgacacgt tttttgtcac aacctttttt cattattatc tgattttcaa
     7681 tgtcaaagca tttagagcta caactaccgt acttaacgac atcaagacag caccaactgc
     7741 tggacttaat atgataccaa ttggcgcaag aattccggct gctagcggaa ttgcaagaat
     7801 attgtaacct gccccccacc agaggttctg gatcattttc tttcgcgttt gcttggctag
     7861 atcaagaaaa tgcaaaatat cttttggatc gctgtttgtc aatacgacat ccgcagaatc
     7921 gattgcgata tctgtccctg caccgattgc catcccgatt gaagcacggg ctaaacttgg
     7981 cgcatcattg atcccatcac ccaccatgat tacttttttc ccttggtcaa ggtatttctg
     8041 gacaacagct tctttatcat ctggtaataa accgccataa tattcttcga tccctaaata
     8101 atctgctact gcttgggcag cgtttttatt gtcaccagta agcatgacag gagtgatttt
     8161 tctttctttc aatgttcgga taaattcttt tgcttctgtt tttactacgt cacctaaagc
     8221 aacgattgca gctagatgat tttctaaaat caggaagctg atggtatttc cttgttcttg
     8281 gtaaggtttc aataatgctt gttctacgtt taatccgaga cgctcaactt ctttttcatt
     8341 aactattttc acttgttgtt ttttgactgt tgcctctagc ccgactcctg aaagattttt
     8401 tagattttca gcttgataag gcttgatttc atgttctttc aaataattca taatgccaac
     8461 agccaatgga tgattggcat tcgcttccaa ggctcctaga tattgaagga tttcattttt
     8521 attgaacgca tcatcaagta cttcgacacc cgttacagta aattttcctt ctgttagtgt
     8581 tcctgttttg tccagcatga tcacatctag atcattggct tgctccagtg cattgcggtt
     8641 tttcaaaagt aatccgtttt tagctgctat tgatgtggaa cgagcaacaa ctaatgggat
     8701 agctaaacct agagcatgag gacaggcgat gataaagact gttaccatac gttctaatgc
     8761 attaggaaga tctgtcaaga acaaccaggc gatgaatgcc aggataccca caattaatgc
     8821 gacataaaac agccattttg ccactttgtc agataatgac tctagcttgg atttttctcc
     8881 ttgtgctttt cgcaccattt ccataacctt cgctagataa ccattttcac cagttccagt
     8941 tacttcgatt tcgatcgttc catccccatt gatagaaccg ccaatcactt tgtcattggc
     9001 ttgttttttg attcctcttg attcgccagt cacagctgat tcgtcaacaa ttgtttcacc
     9061 tttcaagatt acaccatctg ttggcatttt atcaccagac cggacgatca aacgatcacc
     9121 ttcgtttact tctttcaatg aaactgtctc ttcttttcca tctttcgtca agcgtttcac
     9181 tgattctggc agtaattctg ctagtttttg taaagcattg ctggcattcg acacagcatt
     9241 catttcaatc cagtgaccta gtagcataat aacgatcaaa gtagccaatt cccagaagaa
     9301 atccatgacg tgtgtatgtg gattgagcag atttgcgata aacgaataga cactataaat
     9361 atatgcaact gtaatcccca tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa
     9421 ttccatctta gctccactta aaaatggctg accgccatag ataaatagta tagttgctaa
     9481 gatcaatacg agccaatcag aaccaggaaa cgttacttga aatggaaatt ccattcccat
     9541 cattggtgaa aaaagaatga ttgggatagc taggataagc gatagccaaa atttttgttt
     9601 gaaattcccc atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga
     9661 atggtccatt tgtttgctgt ttgctatatc tccatggtta tgtcccatgt gcatggaatg
     9721 atccatctga ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc
     9781 tttgtttact acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt
     9841 tactccatcc cttctcttac tttttcatgt gtcgatttaa gcacagggag ttcaacaata
     9901 cactgacgga actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg
     9961 cggcaaaagg aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt
    10021 ttctcaatgt caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca
    10081 tcagcgtcac atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga
    10141 catcagctaa tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt
    10201 tgccatcttt ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa
    10261 agatatgctt tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt
    10321 ctcctgtcag cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttga
    10381 cttctagttt gatttgatca gccacggcaa tcaacccgat cacttgcttt tcatcagcta
    10441 aaaacatgac tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa
    10501 atgataggtt caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccgttaa
    10561 tcgtaccgct gattccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg
    10621 cttcttgcag ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt
    10681 ctaaagtata aaaaatagat aggacttctt tagaaccgac tagatctgta actttgggct
    10741 ttccttctgt aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt
    10801 ctaatgcttc tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca
    10861 taatagctgt tggagtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga
    10921 cactatggat caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga
    10981 taaacgcgat gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt
    11041 ggattggggc tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg
    11101 tctgcttgcc gacttgtgta acttccatgt gaagtagacc attcgtattg attgtcccgc
    11161 caaagagcgt gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt
    11221 catctatcgc actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac
    11281 ggatgactag ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt
    11341 ttcttatcac ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg
    11401 ttttcgtttt tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac
    11461 tgctgctttc aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca
    11521 atgcaaaggc agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct
    11581 tgattgcatg gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag
    11641 acagcactaa ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta
    11701 acatcatagc aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat
    11761 cgaatagcat ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt
    11821 catcgtacaa aattgcacca tagccgatgt tttctacgct ttggattaat ttttcagtgg
    11881 tcgtcccttc atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca
    11941 caccaggttg tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac
    12001 ctgtaatgac gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt
    12061 ataacccaat ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa
    12121 cttgaccgta ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc
    12181 gcttactgtt tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa
    12241 ttgttgtttc attttttctt cctcctattg atgttgttta cattcacatt gacctgggat
    12301 acagttacat tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt
    12361 gatatcttct tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct
    12421 ttttgcacag atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga
    12481 gacagctgga taataaatga atttttttcc ttcttgttct gtccagatca cttctttttt
    12541 taccaaacga cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa
    12601 gatctgcgtg atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc
    12661 ccactctgaa tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt
    12721 cgtttacaaa tgtaatcatt atcttatgaa tacagattac acttgtaaac aaaaactgtc
    12781 aattattttt gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt
    12841 taaggatgat cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt
    12901 cattgttttc ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt
    12961 tctccgaaaa tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag
    13021 aacgccaaac tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat
    13081 ctgcttcttt aatcctttcc tctaatgtag aagtataatt tccttcaacg atccatgacg
    13141 gattagatac cataaacaat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt
    13201 agtctgttgt atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga
    13261 tcttccttgc gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc
    13321 ttctccctca cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa
    13381 acaagggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DADVXN010000077        13341 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.079, whole genome shotgun sequence.
ACCESSION   DADVXN010000077 DADVXN010000000
VERSION     DADVXN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.079"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            13..159
                     /locus_tag="KB942_001966"
     CDS             13..159
                     /locus_tag="KB942_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBB6852386.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(221..2032)
                     /locus_tag="KB942_001967"
     CDS             complement(221..2032)
                     /locus_tag="KB942_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBB6852387.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2029..2394)
                     /locus_tag="KB942_001968"
     CDS             complement(2029..2394)
                     /locus_tag="KB942_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBB6852388.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2557..2952)
                     /locus_tag="KB942_001969"
     CDS             complement(2557..2952)
                     /locus_tag="KB942_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6852389.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2970..3932)
                     /locus_tag="KB942_001970"
     CDS             complement(2970..3932)
                     /locus_tag="KB942_001970"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBB6852390.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3932..4144)
                     /locus_tag="KB942_001971"
     CDS             complement(3932..4144)
                     /locus_tag="KB942_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBB6852391.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4165..4863)
                     /locus_tag="KB942_001972"
     CDS             complement(4165..4863)
                     /locus_tag="KB942_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBB6852392.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4883..5425)
                     /locus_tag="KB942_001973"
     CDS             complement(4883..5425)
                     /locus_tag="KB942_001973"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBB6852393.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5557..6561)
                     /locus_tag="KB942_001974"
     CDS             complement(5557..6561)
                     /locus_tag="KB942_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6852394.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6558..7547)
                     /locus_tag="KB942_001975"
     CDS             complement(6558..7547)
                     /locus_tag="KB942_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6852395.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7544..8350)
                     /locus_tag="KB942_001976"
     CDS             complement(7544..8350)
                     /locus_tag="KB942_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852396.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8516..9472
                     /locus_tag="KB942_001977"
     CDS             8516..9472
                     /locus_tag="KB942_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852397.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9549..10067)
                     /locus_tag="KB942_001978"
     CDS             complement(9549..10067)
                     /locus_tag="KB942_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6852398.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10155..10304)
                     /gene="rpmG"
                     /locus_tag="KB942_001979"
     CDS             complement(10155..10304)
                     /gene="rpmG"
                     /locus_tag="KB942_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6852399.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10532..10978
                     /locus_tag="KB942_001980"
     CDS             10532..10978
                     /locus_tag="KB942_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6852400.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11173..13068)
                     /gene="asnB"
                     /locus_tag="KB942_001981"
     CDS             complement(11173..13068)
                     /gene="asnB"
                     /locus_tag="KB942_001981"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBB6852401.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4150 a         2830 c         2276 g         4085 t
ORIGIN      
        1 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
       61 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
      121 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
      181 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
      241 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
      301 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
      361 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
      421 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
      481 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
      541 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
      601 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
      661 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
      721 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
      781 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
      841 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
      901 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
      961 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
     1021 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
     1081 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
     1141 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
     1201 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
     1261 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
     1321 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
     1381 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
     1441 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
     1501 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
     1561 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
     1621 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
     1681 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
     1741 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
     1801 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
     1861 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
     1921 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
     1981 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
     2041 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
     2101 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
     2161 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
     2221 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
     2281 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
     2341 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
     2401 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
     2461 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
     2521 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
     2581 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
     2641 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
     2701 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
     2761 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
     2821 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
     2881 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
     2941 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
     3001 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
     3061 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
     3121 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
     3181 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
     3241 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
     3301 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
     3361 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
     3421 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
     3481 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
     3541 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
     3601 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
     3661 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
     3721 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
     3781 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
     3841 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
     3901 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
     3961 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
     4021 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
     4081 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
     4141 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
     4201 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
     4261 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
     4321 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
     4381 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
     4441 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
     4501 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
     4561 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
     4621 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
     4681 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
     4741 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
     4801 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
     4861 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
     4921 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
     4981 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
     5041 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
     5101 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
     5161 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
     5221 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
     5281 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
     5341 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
     5401 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
     5461 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
     5521 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
     5581 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
     5641 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
     5701 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
     5761 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
     5821 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
     5881 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
     5941 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
     6001 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
     6061 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
     6121 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
     6181 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
     6241 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
     6301 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
     6361 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
     6421 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
     6481 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
     6541 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
     6601 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
     6661 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
     6721 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
     6781 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
     6841 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
     6901 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
     6961 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
     7021 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
     7081 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
     7141 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
     7201 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
     7261 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcactccttg catcaatgca
     7321 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
     7381 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
     7441 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
     7501 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
     7561 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
     7621 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
     7681 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
     7741 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
     7801 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
     7861 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
     7921 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
     7981 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
     8041 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
     8101 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
     8161 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
     8221 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
     8281 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
     8341 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
     8401 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
     8461 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
     8521 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
     8581 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
     8641 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
     8701 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
     8761 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
     8821 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
     8881 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
     8941 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
     9001 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
     9061 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
     9121 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
     9181 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
     9241 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
     9301 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
     9361 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
     9421 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
     9481 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
     9541 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
     9601 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
     9661 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
     9721 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
     9781 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
     9841 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
     9901 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
     9961 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    10021 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    10081 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    10141 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    10201 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    10261 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    10321 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    10381 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    10441 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    10501 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    10561 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    10621 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    10681 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    10741 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    10801 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    10861 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    10921 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    10981 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    11041 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    11101 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    11161 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    11221 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    11281 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    11341 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    11401 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    11461 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    11521 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    11581 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    11641 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    11701 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    11761 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    11821 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    11881 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    11941 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    12001 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    12061 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    12121 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    12181 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    12241 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    12301 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    12361 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    12421 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    12481 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    12541 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    12601 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    12661 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    12721 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    12781 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    12841 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    12901 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    12961 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    13021 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    13081 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    13141 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    13201 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    13261 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    13321 gacacgacct aaagatcgtg t
//
LOCUS       DADVXN010000078        13291 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.080, whole genome shotgun sequence.
ACCESSION   DADVXN010000078 DADVXN010000000
VERSION     DADVXN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.080"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            5..2377
                     /locus_tag="KB942_001982"
     CDS             5..2377
                     /locus_tag="KB942_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852402.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="KB942_001983"
     CDS             2438..3898
                     /locus_tag="KB942_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBB6852403.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="KB942_001984"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="KB942_001984"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6852404.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="KB942_001985"
     CDS             6129..6278
                     /locus_tag="KB942_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852405.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="KB942_001986"
     CDS             6353..6946
                     /locus_tag="KB942_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBB6852406.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="KB942_001987"
     CDS             6959..7858
                     /locus_tag="KB942_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBB6852407.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="KB942_001988"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="KB942_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6852408.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="KB942_001989"
     CDS             8724..8984
                     /locus_tag="KB942_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852409.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="KB942_001990"
     CDS             9429..9635
                     /locus_tag="KB942_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBB6852410.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="KB942_001991"
     CDS             9683..9886
                     /locus_tag="KB942_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852411.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="KB942_001992"
     CDS             9902..10339
                     /locus_tag="KB942_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852412.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="KB942_001993"
     CDS             10332..11039
                     /locus_tag="KB942_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852413.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="KB942_001994"
     CDS             11419..11598
                     /locus_tag="KB942_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852414.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="KB942_001995"
     CDS             11923..12186
                     /locus_tag="KB942_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBB6852415.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="KB942_001996"
     CDS             12180..12530
                     /locus_tag="KB942_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6852416.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="KB942_001997"
     CDS             complement(12554..13168)
                     /locus_tag="KB942_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6852417.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4917 a         2198 c         2732 g         3444 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga t
//
LOCUS       DADVXN010000079        12873 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.082, whole genome shotgun sequence.
ACCESSION   DADVXN010000079 DADVXN010000000
VERSION     DADVXN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.082"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1125)
                     /locus_tag="KB942_001998"
     CDS             complement(<1..1125)
                     /locus_tag="KB942_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6852418.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITF"
     gene            complement(1247..1843)
                     /locus_tag="KB942_001999"
     CDS             complement(1247..1843)
                     /locus_tag="KB942_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6852419.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2043..4685)
                     /gene="alaS"
                     /locus_tag="KB942_002000"
     CDS             complement(2043..4685)
                     /gene="alaS"
                     /locus_tag="KB942_002000"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBB6852420.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5073..5504
                     /locus_tag="KB942_002001"
     CDS             5073..5504
                     /locus_tag="KB942_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852421.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5549..6898)
                     /locus_tag="KB942_002002"
     CDS             complement(5549..6898)
                     /locus_tag="KB942_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6852422.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6968..8131)
                     /locus_tag="KB942_002003"
     CDS             complement(6968..8131)
                     /locus_tag="KB942_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6852423.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8260..8583
                     /locus_tag="KB942_002004"
     CDS             8260..8583
                     /locus_tag="KB942_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852424.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8766..10115)
                     /locus_tag="KB942_002005"
     CDS             complement(8766..10115)
                     /locus_tag="KB942_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6852425.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10184..11137)
                     /locus_tag="KB942_002006"
     CDS             complement(10184..11137)
                     /locus_tag="KB942_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBB6852426.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11239..12429)
                     /locus_tag="KB942_002007"
     CDS             complement(11239..12429)
                     /locus_tag="KB942_002007"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBB6852427.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3698 a         2712 c         2224 g         4239 t
ORIGIN      
        1 aaacgtaatt tccaattggt aatctccgat tttttcttct atttgactac gtaagattct
       61 ttctagttct gtatcaacaa actggaattt ctcagggacc actacatcaa attgcagcgt
      121 ttttccttga tgaattactc taaaatcatg aatattaagc cctgtacctg tttcaagaag
      181 aatttgcttt aactgccgca agatattata gtatttttcg ttatcaacag gcattggatc
      241 caagtgacag acaagttcca catccaaaga ctttttaaag tctttttcta tcagatcaat
      301 aatcttatga gccacattca aattcattga ttcatctact tcgatgtgga ctgatgcaaa
      361 gcgtttattg ggtccatagt tatgcaccaa taaatcatga tagccaagta tatcatcgca
      421 agcatttaaa tgctcttcca tttcttcaat ctctgctggc gttgggcgac ttcctaatag
      481 ttcgtataca aaatttcgaa gcatcgtcaa tccactataa aggatatata atgctaaagc
      541 aaaacctata tatccatcaa tacgccatcc agttacccat tcgattgctg ctgaaagcaa
      601 tacagccagt gtagtaaaga catcattata actatctgtt gctgtagctc tcaatgtttc
      661 agaatcaatg gattgcgcaa taacctgata cattttgcct tgtgcaaatt tcaaaaagat
      721 ggaaatgatt aggacaaaaa agacgatggg tgttaaagag acattttctg gatggatgat
      781 tttttcagca gaagactcta aaaattgaaa tcctacataa gtaatgatga tcgaaacaaa
      841 taacccactg atatattcga agcgttcgtg tccataagga tgttcctgat ctgctggctt
      901 tgcagcaatt ttaaatccga tcaaagtcaa tatggatgaa gcagtatctg atagactgtt
      961 cactgcatca gccataatgg agacacttcc tgaaaaaagc ccgatcatta gcttagagac
     1021 aaacaagatc agattcgtaa ccaatcctaa gatacccgct agtactcctt tttttgtccg
     1081 ctgttgcggt atttgtttct ttttttcttc ttgtttctta gccaagtttt ttaccccttc
     1141 tagtttgctt gttgctcagg taaaaagatg gagaggacga ttgttttcct ctcctctctt
     1201 cctttttcta tctatcgtgc gtcctattac tattaaaaga tggtcattat tctggtttca
     1261 acggtgttgc tgcaaataac gcttttgcta gtaatggtgt tatgattccc gcacctattg
     1321 cttctggtat cccattgatt gctgcaaccc ctgctaaggt tttaaataaa agagcagagt
     1381 ctacaccata ggcatctgcc gttgcacctg tataaaataa tcccatgaat cctaacacta
     1441 agactgtatt gaccaatgaa cccagtaccc ctgcaaaggc tgagctgatc gttattgatt
     1501 tatttttttg ataaatccag tggaaaacca agcctgctac taagccaaca agtactctgg
     1561 gaactaccga gataatcgga ttcgtgaaca ctaatgtatc aaatggcgtt gttggcatgg
     1621 cataggcacg aacaattgta gcgattcccc aaaacaagcc aatcaccatg ccatcttttg
     1681 ggcctaatac tactgcagca ataattactg tgatatggat aattgttaaa ctaatgaatc
     1741 ctaatggaat aaaccccagc caaggaacca tcgcttgtac gatgatgatt gctaacaaga
     1801 tcgcacgtaa gactagtcga aaggtcttat ttctactatt cattttttca ccccttttta
     1861 tttatactaa tcgtaaaaat ttctgtcact ttcgtcaact aaagaccatc cgaatccgac
     1921 catttgatta gatgcgccat ccaattgaaa aaagtatgag ggcaagactt gtttgtcttc
     1981 gcccccataa tcatgtttcg ttcaatgttt gaacttttgt ttttctgtca aacgtaaacg
     2041 ccctattgtt gcgcaaccca ttctttcgca gctttaagtg catctggaat accagcagga
     2101 tttttcccgc cagcttgtgc catgtctgga cgtccaccac cgccaccgcc tactttcggt
     2161 gcgattgctt taatcaagtc gccagctttt agtccagctt catttgattc tttcgtcatt
     2221 gccgctagca gactgacttt tccttcttgt tcgtttgcta aaacaagaat atctgaaata
     2281 tttttttgtt tccattggtc agctaattga cgcagctgat tcatatcttt cacttttact
     2341 tgtgctgcaa tgattgtttt tccattgatc gtttctggat ctttgaatac atcaccagct
     2401 tgttgattcg ctaacttgct agctaattgt tcattttcct tttgtagctc tcttacttgc
     2461 tgttgcaatt gttccgcttt tgaaacaact tctttcaatt gaggtgattt aacaattgtt
     2521 gctacttgtt ttagttgttt ttcttcttct tcaagtagtt cgtacgcttc tttacttgtt
     2581 acagcctcga ttcgtcgcac gcctgcacca attcctgatt cagaaacgat tttgaagatt
     2641 ccgatgtttt ctgtatttgc tacatgtgtg cctccgcaca gttcgattga ccagccgcca
     2701 atatttacta ctcgaacgtc atgtccgtat ttttcaccaa acaaagccat tgcacccatg
     2761 tttttggctg tatcgatatc tgtttcaatt gtttcaactg ggatagcttc ccagattttt
     2821 tcgttgacga ttctttccat ttcagctaat tcatctgaag tgatttgacc aaagtgggta
     2881 aagtcgaatc gtaaatgtcc aggagcgact aaagaaccag cttgatttgc atgctcgcca
     2941 agtacttctt tcaatgcctt gtgcagtaga tgggttgctg tatggttttt gatgatccga
     3001 ttgcgcatcg attgatcgat caatagatga taggtttgtc cttcactcat tggtgctttt
     3061 acttctacgg tgtgcataaa ttgaccatta ggcgcttttt gaacatctgt aacaactgct
     3121 acgatttcgc ctacttcatt catgatccat ccttgatccg ctacttgccc acccatttct
     3181 gcatagaaag gtgtttgatc aaagatcatt tgagcttcac cttctgcgac ttcattcaaa
     3241 agagactcac ctttaacgat gaccaataat tgactatctg attcttgttc tgtgtatcca
     3301 atgaatttac tttctacttt gatatctgtc aataaagcag attgtacccc cattgatttt
     3361 tcagtactgc gggcagagcg agcacgttcg cgttgtgcat ccatttcttt ttcaaaacca
     3421 gcatgatcaa ctttcagtcc ttcatcttct gccacctctt ccgttaattc taccgggaag
     3481 ccatatgtgt cgtatagttt gaagatgtct tttcctgcta gagtcgtccc gttttcgttt
     3541 tttacttctt ggatcacttg atttaagatt tccaaacctt cattgattgt ttcattgaaa
     3601 cgttcttctt ctgtacggat aactttttca ataaattctt tttgttgaag tacttcagga
     3661 taataactta ccatgatttc cccaacaaca gggactaaac gatataagaa tgcttgttca
     3721 atacctaatt ttttcccatg catgacagca cgacgaagca aacggcgtaa gacataccct
     3781 ctcccttcat tcgatggcaa tgcaccgtcg ccaatagcaa atgaaagggc gcgaatatga
     3841 tctgcgatta ctttgtaaga aatatctgtt actgggtttt caccatattt tacttgtttg
     3901 cttagctttt ctacggcgtg gataataggc ataaacaagt ctgtctcaaa gttcgttgac
     3961 gcatcttgaa cgattgagac catgcgttcc aatcccatcc ccgtatcaat gtttttatga
     4021 ggaagtggtt caaatgtgtt atcaggctta tggttgaatt ctgaaaatac taagttccag
     4081 atttctaaat aacgttcatt ttctcctcct ggatagtttt caggatcgtc ttctgctaaa
     4141 tcgttataag cttctccacg gtcatagaaa atttccgtat ccggaccaca tgggcctgca
     4201 ccgatatccc agaagttgtc ttctacatca ataatgtgat ctagtggcag gccaacttct
     4261 tcatgccaga tgcgttttgc ttctgtatct tttggataaa ctgttacata aagtttttct
     4321 ggatcgaatg ccatccattt tggatcagtc aaaaattccc atgcccagtg gatggcttca
     4381 tttttgaaat agtcaccaat cgaaaaatta cccagcatct caaacatcgt atggtgccgt
     4441 gctgttttac cgacattttc gatatcattt gttcgaatag atttttgggc atttgtgatt
     4501 cttggatttt ctggcacaac tgaaccatca aaatattttt tcaatgttgc aacaccagag
     4561 ttgatccaca ataaggttgg atcgttcacg gggatcaaag acgcactagg ttcaatcgta
     4621 tgtcctttag attggaagaa atcaagaaac atttgtcgaa cttgtgcact acttaattgt
     4681 ttcatgtcat tacctgcttt ctataaatta aatacaaaaa aacaccatca cagccaagga
     4741 cgaaaactcg cggtaccacc ttgattgcaa tgatgtgtaa tcattacctc ttaaactcga
     4801 taacgctgag ctgcgtcggt atttctacca aaatagaaaa agggagcaat ttgattagtg
     4861 actattcttt tttcagccaa gaaagaattt tctgtgagtc ttaaaaaaca aatcatatcc
     4921 ttttcgtact taattagtat acgcattcta ttgatcactg tcaatatctc aatttttatt
     4981 ttcatttcct tttggtcata ctccacgatt tttggtacga taagaataaa ggaatcaata
     5041 catctctgct attcatgaca aggaggaata tgatgaaaaa aatagttgga gcggatgaat
     5101 ggatacgcgc agctagctgc tttttgagat atcaagtttt tgtattagaa caagggattc
     5161 ctgcagaact agaattcgac ggaaaagata tcccgcaaca aacctattat ctatttcttg
     5221 atcaaaaaag accagtagct actttgcgtt atgaaaagaa aacacctact tgcttcaagc
     5281 cggaacgatt ttgcgtagca aaagaatacc gaggaaaagg gttaggcggt cagttgcttg
     5341 aagctgctga aaaacgggcg gtttcagaag gttgtcagac tagttatgta gtagctgaaa
     5401 tgacggctgt agatttttat gaaaaacaag ggtatcatac ttgcacagaa ccttttattg
     5461 aagatgaagt actttgtatc ggaatgaaaa aaagtctagc ttaaaaacaa gagattgtga
     5521 cacgcctttt gtcacaacct cttgtttttt atttatgtga atttttctta tttttacgag
     5581 cagtacggct ttgctgtctg cgttcgattt tgcgttcttt tttatttttt tctgcaattg
     5641 cccaatcgat ttttttctta taaccaggct tgatcttttt ctttttcttc ttcacaagac
     5701 caatcagcgt gggatctaat acatcttttg atttttctcg tttggcacgt ctattacgat
     5761 cgtatgtttt gacgatttcc ccgtttttga tttcttttgg ttcaaatgag acgcccaatt
     5821 tttctatcgc atcaatggct tgctcatcat ttggcgaata taacgtaatg gccgttcctt
     5881 ttaagccatt acgtccagta cgtccgacac gatggataaa gaagtctaga tcttctggaa
     5941 tttctgcatt aataacatga gagacacctt cgatatcgat ccctctagca gctaaatcag
     6001 tcgctacaac atattgatac tccagattct gtacttgtcg catcactcgt tttctctctc
     6061 ttggggtgat gtctccatga atctttgcta ctttcaagcc ttggtttttc aagtaatcgg
     6121 caatttcatc cacatgctgt tttgtattgg caaatacgat tgctaaataa ggatgaccaa
     6181 ttgtcagcag ttgataaatc atttgattct catctttacc ttttgttgaa atcagccaat
     6241 tttcaatgtc ttcagaaata actgcttttg gtttgatttc ttcaatgatt ggattttcca
     6301 tgtattttcg taagaatgga cgaagttttt ctggaatagt ggcagaaaaa actaaaaact
     6361 gcagcttttc tggtaatcga cttgcaattt ggtctacttc atttaaaaaa cccatatcca
     6421 atgtcatatc cgcttcatcg acaacaaatg caaatgcggt gtgaatcttc agcgcttgtt
     6481 cattcatcat gtctaatatt ctaccaggcg tgcctactac aacctgtggc tgttggtgat
     6541 gcagacgatt taattgacgc tgtttgtctg taccgccgac aaaattcgag acacggatct
     6601 ccggtgtaga aaatttagcg atttgtgtag ctgcttcata gatctgtgca gctagttccc
     6661 tacttggagc tgtaataaca acttgtactt cctctttttc tgcattgatt ttttcaaaca
     6721 aaggcaacaa aaacgtatgt gtttttcctg taccagtttg agattgccca atgatatttt
     6781 ttccttttgc aattaaagga atcaaacgtt cctggacctc tgtgggctgt ttgaagccct
     6841 ttgcggttaa cgcttcattg ataaacggtt tgaagcggaa tatatcaaaa ttattcattc
     6901 gtgcacctct tctctttaaa ttatcacgac tttggcatta taacacagac aaagcgtttt
     6961 tgctcagcta atttcgagaa tcatcatcct ttgtttggcc aatcgtgcta acagtatatg
     7021 tgtgactagc tttttcataa aacagaatcc gtttttttct caaaaatgat agcaagatat
     7081 ttaaaataaa aataccttgt agtaacaaga caccgagaga aatgaatact gcactcatct
     7141 gcaacaccgt attcgttgtc gaattcatga tttgctgaag caactgcccc aaccagctta
     7201 aaccataaaa aagaaagtac agatacccgt agcggatcat caatgctttt aaagaaattc
     7261 gttcgcggtc ttcttccacc aaacggatac gcacaatttt tttgccaagt gtccatccat
     7321 tggttataaa cggtacaatc ataaagtata cccctacttc taataaaagc caccagatat
     7381 gagaagtaac tgcccctgaa tcgaacgcaa ttttaaagaa aatcgccgct accaaattaa
     7441 taagagataa aaatgtccag tcgatcaaat aagcgaccaa gcgtcgagtc aatgagacag
     7501 tctttccttt ttcataagaa agttcatcca ttcgtcttct tgtaggaagc ataaatgtaa
     7561 ataacggcgt aacgacaaaa ccaatgattc cacccaatgt atttgtcact aaatcattca
     7621 catcaaataa gcggtatggt cttgggtaga taaaataaag tccacttagc tgcgtcaatt
     7681 caaaaaatag agaaaggcag aaactagcaa tcatcgtttt cataaaagaa agccggaaat
     7741 aatatcttaa ataaatgcca aacggtatga gtaataaaac attaaaagct ggttccaaaa
     7801 atacactctg cttcattgcc ggcaggtagg tactaggttc agacaagacc aataccgtgt
     7861 tgtctctgaa atgttgccat gaagcaaata aatgcagctc catctttggc cctgtcattt
     7921 gggcaaccgt ctcctttgcc ggaagtggta agatcaccaa aaaatacgca cacaataaat
     7981 aaaaaacgaa agaatacaaa ataactgccc ttgttactag aaatgttcca tactttcgat
     8041 attcataaat aaaaaaaaca gatgagatcg ctaaggctaa aaatggaaaa acaatcagcg
     8101 caaaacgaat cggttcgata taacttgcca tatgactcct ccttgattct ttgtatctct
     8161 cttatcttag cagtcctttg ctgtttcaag caagtaacga ggctattatt tctcatttct
     8221 ttcaaagtaa agtaagataa acacaaaggg ggtgtcgaga tgctgaatat gtactttgtt
     8281 tttggcgtac ctattttttt actgttcctt tatgctacga tcgcttatgt aagaaaaaga
     8341 actacaattc attatttagg atttattctc ctgattattt cgggtttcat gctagtcttc
     8401 aatcttcaga cttggcagca agcacttctt gaaatggata aaatgactcc acatgctttg
     8461 tctaaagtgc ttggctaccc tgtctatctg atttggcttc ccattttcat ttctggttgc
     8521 ttggttttat taaatattta tcgcggcgta cgccgaatag ttcagttacg caaatcaaag
     8581 taaaagacag aaatcggttg agcccgattt ctgtctttta ctttgctatt tcatcgtaga
     8641 caaaacgaca tttacgtgat caaaattaac gagtactttt gacatttctt ccacgtactc
     8701 ctcatttaaa taaaatattt tgtttggcag agaagtgcag ttctaataaa attcgaatgt
     8761 tttagctatc tacgttttaa atagagataa atcgggatac cagcaagtgt caatactatc
     8821 cccatcgaag ctaaaaaggt ctgagtgata agcgtgcttg atacgataaa agtccctcca
     8881 atgattgcta ctagtggaat aaacggatac attggaactt tgtatggtcg aaacaaatca
     8941 ggttcttttt tacgaagaag aatgacacca acaaagacca tcgtataaaa aatccaaatg
     9001 acaaagatca gcatatccgt tagtgtatca aaaccaccga tcatcatcat gccaatagcg
     9061 atgactagct ctaagatacc tgcggctaca ggaactttag ttttatcatg aagtttagca
     9121 aagagcttgc taaaaggcag gttgttttct tttgccataa cataagggag acgcattcca
     9181 gtaagggtat agccattgat ggttccataa acagaaatca agataccaac tgtgaccagt
     9241 cttccaccga agccgccaaa aatcattttt gcaacgtcac ttgccgcatt gctgtttcct
     9301 gcgactccgt cgatagatgc ggttcttaag aacaccgcat ttactaataa ataaacgatc
     9361 atgattccga tgattcctaa agaaattgct tttggcaaat ctttcgccgg ctttttcaat
     9421 tctcccgaaa tatttcctac gtgtatccaa ccgtcgtatg cgaacatcgt tgctagcaag
     9481 cccgctccca aggctgagaa aaatgaaaga ttttctcctg cttggatcgg aaacagttgg
     9541 aagtctactc cttcttgtcg gaacaaaccg aaaatgacga tcacaaacaa aggaataagc
     9601 ttgcagacaa gcgtaatcga ctgaaaagcc cctccagctt tagaacctaa aaaattgatc
     9661 aacaggatcg atacagcagc tgtaacagct acgggaacaa tcattgactg ggaaagacca
     9721 aatagattga cgaactgtgt accgaaaatg atcgacaagg ctgctacatt tgctggaaaa
     9781 taaatcacga cttgcgccca gcccaataaa aaagctgctg tatttccgta aatccgttcg
     9841 atatatttaa tcatccctcc tgtttctgga atagcagctg ccagttcagc tcctgttagt
     9901 cctgcacaga cagaaatcat accaccgaga aaccaagaaa acatatgcaa actggcggat
     9961 cctgtaactt ctgctacgct tgcggcttta aaaaataccc ctgcaccgat cactgtgccc
    10021 ataacagtcg ataaagcagt aaaaaatccc atcgtacgtt ttaattcatt ttctattttt
    10081 acttctttta ctggcgtggc ttgatattct tccatccctt gtcccctttc ataccatagc
    10141 caatcccaaa agtataaaaa actttttgga tgtggttctt gttttatacc gtctctacca
    10201 atttttcttt cagtttttcc aacatatcct ttgtcatcac atctaagtcg tatttcggcg
    10261 aaaagcccca ttcctcttgc gcacaagaaa catctagtga atttggccaa gattctgcta
    10321 ttgactgacg tacaggatcc acatcataat ccatttcaaa atctggtatg attttttgaa
    10381 tagatgcttt gatctcttcc gggtcaaaag acatcgccga tatattgaag gcatttcgat
    10441 gaaccaatcg atcaggatct gcattcatga gttttataat ggcatcgatc gcatctggca
    10501 tatacatcat atccattgat gtaccttgat caatgaaact agtgtacttt ccttctttga
    10561 ttgctgaata ataaatatcg actgcgtagt ctgtcgtacc gcctcctggc aaggttgcat
    10621 aagaaatcaa tccagggaaa cggacaccac gagtatctac gccatatttc acataataat
    10681 aatcacatag tagttctcca gctaccttcg ttaccccata catggttgta ggacgctgaa
    10741 gagtatcttg tggggtatga tcttttggag tatttggacc aaacgcgccg attgaacttg
    10801 gcgtaaaaaa tttcagctgg aattctctag cgacttccaa tgcattgacc agcccagtca
    10861 cgttcaaatt ccaggctaat tgtggttttg cttctgcaac agctgacaaa agagcagcca
    10921 aatgaatcaa tgtgtccact tgatattgtt ctgctatgct gcgcattttt tcgtaatcca
    10981 ttacatctag gatctcaaag atatcgtttt tctcaatgac ttcaggcatg cgtatatctg
    11041 tcgcaatgac attttcactg cctagttctt gacgtagccg tgtgatcaac tctgaaccaa
    11101 tttgtcctaa acatcctgtc accaatactt ttttcatgat gatcctccct gatctttttt
    11161 actactatct tcagatgatt aacaatatat gcgtgaacat gttctctaat tttaaataga
    11221 cgaaaaaact aaatttagtt aaataatttc catttctttt cctatttttt gataaactgc
    11281 tactgcttca tcaagcattt ccctcgtatg ttccgcagtc ggcatatttc tgatcctgcc
    11341 tgtcccaaga ggcacagttg gataaacgat tggtttagca taaatcccat gttcgatcag
    11401 cttcttagag aaagtctgag tcactttttc gtctcccaaa atgactggag taataggtgt
    11461 ttcagacata ccgatgttat agcctacttt tttcaattct tctttaaaat aattagcatt
    11521 ttcccaaact ttttcgacaa gttccggatg ttcttgcatc aaagtaatcg aagccaatgc
    11581 agctgcagct gcacctggag ttagcgaagt agaaaatagg aatggccgac ttctagcttt
    11641 caaccagtcg attagtgttt tcgatccagc tacataaccc ccaacaacac ctatagcttt
    11701 cgataacgtg cccatttgca tatcgatttt gtcgcttaat ccaaaatgct tgacggttcc
    11761 tgcacctttt ccagttacgc ctgagccgtg cgcatcatcg acgtaggtga tcagtccata
    11821 tttttcaaca atcggaataa tttccggcaa tcgtgcaatg tctccatcca tggagaaaac
    11881 accatctgtg atatacatga tttttttata tttcttactt tctatcgctt cctttgcttt
    11941 tttctcaagg tctttcatat cttgatgttt aattcggatg atttttgctc cggataagcg
    12001 gcaaccgtca ataatagatg catggtttaa ttcatccgat aagatagcat cttcttttgt
    12061 cattactgca gagatagcgc ccatattgca gttgaatcca gattgaaagg cgattgctgc
    12121 ttctgtccct ttgaattctg cgattttttc ttctaattgc tgatggattt tcagcgaacc
    12181 attgatcgtt cggactgctc ctgctcctac accataagtt tcggttgctt caatacatgc
    12241 ttttttcaat tcttcgcgat tggcaaatcc aagataattg ttggatgcta gattgatcat
    12301 cttttttccg tctacgatga tacaagcacc attttcactt tctagtatgt cgatcgtatt
    12361 gtataacccc ttttctttca gttgatccaa ttctttctct aaatacattg ttagttcttt
    12421 ggaagacatt gtctccacct ccatattcat ctgtcttgca gaacatacca aacatatatc
    12481 gtgtcattca tctacaaagc aagtatgcta tatttttgat aacgcttaca ctgattatga
    12541 gaattattta accacatttt ttatttacag tatcttttta ctttcaaatg acttatatag
    12601 tatgaacaat taccttttag ataatgatct attttactct acctgggcag ataaatataa
    12661 cacaaccatt gatagaagcc ctttcgttaa aatcaattgt attctgaata tttaatttat
    12721 ttattgcata gaccaataaa aatacataaa atttccatct tcgtttatca gcaattttta
    12781 attttactct ctgaatattt cttaaaagtt ttacttgttg aattcgaaaa aaggactgtg
    12841 acaaaagtct tgtcacagtc ctattcttcc gaa
//
LOCUS       DADVXN010000080        12852 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.083, whole genome shotgun sequence.
ACCESSION   DADVXN010000080 DADVXN010000000
VERSION     DADVXN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.083"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            95..1381
                     /gene="efmA"
                     /locus_tag="KB942_002008"
     CDS             95..1381
                     /gene="efmA"
                     /locus_tag="KB942_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBB6852428.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1519..2118)
                     /locus_tag="KB942_002009"
     CDS             complement(1519..2118)
                     /locus_tag="KB942_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBB6852429.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2522..3643
                     /gene="mnmA"
                     /locus_tag="KB942_002010"
     CDS             2522..3643
                     /gene="mnmA"
                     /locus_tag="KB942_002010"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBB6852430.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3889..5310
                     /gene="arcD"
                     /locus_tag="KB942_002011"
     CDS             3889..5310
                     /gene="arcD"
                     /locus_tag="KB942_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBB6852431.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5324..6442
                     /locus_tag="KB942_002012"
     CDS             5324..6442
                     /locus_tag="KB942_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBB6852432.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6502..7794)
                     /locus_tag="KB942_002013"
     CDS             complement(6502..7794)
                     /locus_tag="KB942_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6852433.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7926..8504
                     /locus_tag="KB942_002014"
     CDS             7926..8504
                     /locus_tag="KB942_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6852434.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8633..9010
                     /locus_tag="KB942_002015"
     CDS             8633..9010
                     /locus_tag="KB942_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBB6852435.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8943..11015
                     /locus_tag="KB942_002016"
     CDS             8943..11015
                     /locus_tag="KB942_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6852436.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11226..12173)
                     /locus_tag="KB942_002017"
     CDS             complement(11226..12173)
                     /locus_tag="KB942_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6852437.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12419..>12852
                     /locus_tag="KB942_002018"
     CDS             12419..>12852
                     /locus_tag="KB942_002018"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBB6852438.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4141 a         2337 c         2742 g         3632 t
ORIGIN      
        1 tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac
       61 gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt cggtcgtttt
      121 aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt caggaatcac
      181 tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg gttcggcgac
      241 aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa gcccatttgt
      301 aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg atattattgt
      361 agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct tcccgatttg
      421 gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat
      481 ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga atggacagtt
      541 aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg ctttgttttc
      601 agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt ttggtgtcgg
      661 cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag ttccgcttac
      721 tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag ggttatggta
      781 cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc
      841 attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag tggaagtagt
      901 ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa catggaaaga
      961 ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg gtgcaagcgg
     1021 attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg caggagcagg
     1081 ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga gttatgaatc
     1141 gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg caggtccaat
     1201 tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt
     1261 ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa
     1321 ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa aagacgaata
     1381 gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc ctaatgttcc
     1441 tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt gtttaatact
     1501 tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat agatttcttt
     1561 gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga gttctctttc
     1621 cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat gatggatatg
     1681 tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat gagtcaagta
     1741 atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa atttgggact
     1801 tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc gactgtcatg
     1861 gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca aaatttctgc
     1921 atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa catggtcggt
     1981 ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat ccacatttgc
     2041 aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa agtgcatatc
     2101 actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa acaaaatcac
     2161 ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg tgcagcttta
     2221 agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta ttccttattg
     2281 ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc tcaaaagatg
     2341 agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag aacagtattt
     2401 ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat gtaaaatact
     2461 gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg atggtgatat
     2521 catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg tcgactcatc
     2581 tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat tcatgaaaaa
     2641 ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata aggatgtagc
     2701 gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa aagaatattg
     2761 ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac ctaacccaga
     2821 cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa tggatcttgg
     2881 cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa acggcgtggt
     2941 ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta gccaattatc
     3001 acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat cagaagtacg
     3061 ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa cgggaatctg
     3121 ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag caaaaaaagg
     3181 aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga tgtattatac
     3241 gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag aaccctggtt
     3301 tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt tccatcatcc
     3361 tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga atgaaccaat
     3421 gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac
     3481 agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat
     3541 cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg gtggtttgat
     3601 cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat taaatgaatc
     3661 aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag ctgttttttt
     3721 gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa agttcataat
     3781 atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg
     3841 aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa
     3901 tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc
     3961 ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt
     4021 tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga
     4081 gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa
     4141 ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct
     4201 tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa
     4261 aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac
     4321 aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca
     4381 ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat
     4441 ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg
     4501 ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca
     4561 agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt
     4621 ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct
     4681 caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg
     4741 ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg
     4801 ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg
     4861 ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact
     4921 gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt
     4981 gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa
     5041 gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta
     5101 gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt
     5161 gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt
     5221 acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg
     5281 ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt
     5341 cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag
     5401 ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa
     5461 atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt
     5521 tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac
     5581 gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca
     5641 tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc
     5701 cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct
     5761 agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac
     5821 aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga
     5881 tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc
     5941 gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat
     6001 tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa
     6061 gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc
     6121 attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt
     6181 gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt
     6241 ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt
     6301 attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc
     6361 ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga
     6421 gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca
     6481 agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga
     6541 gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg
     6601 tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc cgcgataacc
     6661 aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa
     6721 agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa
     6781 tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc
     6841 taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa
     6901 accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc
     6961 ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt
     7021 cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt
     7081 ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa
     7141 gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc
     7201 atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa
     7261 ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt
     7321 tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt
     7381 cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag
     7441 acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc
     7501 cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc
     7561 ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca
     7621 aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg
     7681 catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt
     7741 ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac
     7801 tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta
     7861 tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt
     7921 attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg
     7981 actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag
     8041 ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg
     8101 tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc
     8161 ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg
     8221 ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg
     8281 ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc
     8341 aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt
     8401 atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca
     8461 gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt
     8521 gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt
     8581 cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca
     8641 agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga
     8701 tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca
     8761 atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc
     8821 cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata
     8881 caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt
     8941 ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc
     9001 ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg
     9061 cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct
     9121 ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa
     9181 caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag
     9241 tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg
     9301 tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca
     9361 aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc
     9421 ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga
     9481 agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag
     9541 accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag
     9601 ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag
     9661 aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag
     9721 aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag
     9781 ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg
     9841 agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga
     9901 aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt
     9961 atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg
    10021 atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag
    10081 agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata
    10141 aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa
    10201 atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat
    10261 gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc
    10321 ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc
    10381 cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc
    10441 ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt
    10501 tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa
    10561 agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg
    10621 agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag
    10681 aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca
    10741 attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc
    10801 aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat
    10861 acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac
    10921 atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat
    10981 tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtgatag
    11041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    11101 aaatttcggc ttatttccga agaagctact tctggaacac catttattcg gaaaaaagga
    11161 ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac ttcatttgac
    11221 gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc tcttatgata
    11281 taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc gatgattcca
    11341 agacataaca attggattcc accaatgaac aggaagatcg agaccatgga aggccatcct
    11401 gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc aataccagaa
    11461 ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc cgagaagttc
    11521 acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt ttcgccagcc
    11581 acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca gctaaataat
    11641 ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat ttgacgagtc
    11701 atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt attgatcaac
    11761 tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc
    11821 cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc aggaagcagt
    11881 tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt agaagcttgc
    11941 aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac aaagcggaca
    12001 taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt tgatccgtcg
    12061 tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa
    12121 agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt catcgtttct
    12181 atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa ccttaaataa
    12241 cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt
    12301 tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat tagttggcaa
    12361 gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag caaacaaaat
    12421 gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg gattgactgc
    12481 tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt taacgcgcac
    12541 acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg ttcctttatc
    12601 tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta
    12661 tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga aagctgtgcg
    12721 agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt gtcatcgagt
    12781 gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg gggttgaaat
    12841 caaacgccaa tt
//
LOCUS       DADVXN010000081        12732 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.084, whole genome shotgun sequence.
ACCESSION   DADVXN010000081 DADVXN010000000
VERSION     DADVXN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.084"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB942_002019"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB942_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6852439.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="KB942_002020"
     CDS             complement(322..891)
                     /locus_tag="KB942_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBB6852440.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="KB942_002021"
     CDS             1077..1979
                     /locus_tag="KB942_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6852441.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB942_002022"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB942_002022"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBB6852442.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB942_002023"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB942_002023"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBB6852443.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB942_002024"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB942_002024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBB6852444.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="KB942_002025"
     CDS             8547..8732
                     /locus_tag="KB942_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBB6852445.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="KB942_002026"
     CDS             complement(8794..9990)
                     /locus_tag="KB942_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6852446.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="KB942_002027"
     CDS             complement(10160..11596)
                     /locus_tag="KB942_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6852447.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="KB942_002028"
     CDS             11889..12698
                     /locus_tag="KB942_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6852448.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DADVXN010000082        12481 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.085, whole genome shotgun sequence.
ACCESSION   DADVXN010000082 DADVXN010000000
VERSION     DADVXN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.085"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(130..777)
                     /locus_tag="KB942_002029"
     CDS             complement(130..777)
                     /locus_tag="KB942_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBB6852449.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="KB942_002030"
     CDS             complement(1056..1559)
                     /locus_tag="KB942_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6852450.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="KB942_002031"
     CDS             complement(1682..2446)
                     /locus_tag="KB942_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBB6852451.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="KB942_002032"
     CDS             2553..2828
                     /locus_tag="KB942_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBB6852452.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="KB942_002033"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="KB942_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6852453.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB942_002034"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB942_002034"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBB6852454.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="KB942_002035"
     CDS             complement(5605..6063)
                     /locus_tag="KB942_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852455.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="KB942_002036"
     CDS             complement(6060..7523)
                     /locus_tag="KB942_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6852456.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB942_002037"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB942_002037"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6852457.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="KB942_002038"
     CDS             8598..9347
                     /locus_tag="KB942_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6852458.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9614..11092
                     /locus_tag="KB942_002039"
     CDS             9614..11092
                     /locus_tag="KB942_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBB6852459.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(11160..12251)
                     /locus_tag="KB942_002040"
     CDS             complement(11160..12251)
                     /locus_tag="KB942_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBB6852460.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         3895 a         2531 c         2106 g         3949 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaagt tattcacaat ctataaagtt actttaaata acaaaaaaat
     9481 aattgaaaat acttaaaatc aaaaaatatc tttttaatca ataaaaaaat tgacagtttt
     9541 attaataata agctattctg ttatagaaga aaaaattgtt tgttattatt agcaacaaaa
     9601 aaaggagtgc atcatgaaaa agttatctca aaaacgttta gctcaaatca ttattgattc
     9661 tagaaaatct ctgaaaatga cacaacaaca acttgcagta gctactggta tcaaccgtgc
     9721 cttaattagt cgattagaat ctctcgattt tatcccgtca atacaacaat tagaatcatt
     9781 agcagaagtt ctaaattttg aaccaacaga tctatttata aatgaatcaa ataaatcaaa
     9841 cagaacaaaa catcagtcga agaatattgc tgtagccggt actggttacg taggtctttc
     9901 cattgctact cttcttgccc agcacaatca ggtaacagca gtagacattc ttcctgaaaa
     9961 agtaaaacaa attaacaata agaaatctcc aatacaagac gattacattg aaaaatatct
    10021 agctgaaaaa aatcttaatt taattgcaac gactgatggt gcagccgcct atagagaagc
    10081 cgattttgtt gtcattgctg ctccaacaaa ttatgatagt aaacaaaatt attttgatac
    10141 ttctgctgta gagtcagtaa tcaaattggt gttagaagtc aatccaaagg caataatggt
    10201 catcaagtcg acggtacccg taggctatac cgaaagtatt cgtaaaaaat acaatactaa
    10261 aaatattcta ttcagcccag aatttctcag agaatctaag gcattatatg acaatctata
    10321 tccttcgcga attattgtcg gcacggacca aactgacaaa actttacgag aagcctctca
    10381 cacttttgca gatttactcc aagaaggagc aatcaaaaaa gatatcgaaa ctcttttcat
    10441 gggattcaca gaagctgaag cagttaaatt attcgctaat acttaccttg cacttcgagt
    10501 atcttacttc aatgagctgg atacttatgc agaaatgaaa ggactaaata cacaagaaat
    10561 cattcacggg gtttgcttag atccaagaat tggcacacat tataataatc catcttttgg
    10621 atatggaggc tattgtttac cgaaagatac aaaacaactt ctcactaact ataacgatgt
    10681 tcctcagaat atgataagtg caattgtcga tagtaatcgt acaagaaaag actttatagc
    10741 agatcgagtg ttagaacttg ctggcacgta tgaggctaac agttcgtgga atgcagaaaa
    10801 agaaaaagag gtagtcatag gtgtataccg tcttacaatg aagagtaatt cagacaactt
    10861 ccgtcagtca tctattcaag gagttatgaa gcgaattaaa gcaaaggggg caactattgt
    10921 aatttatgaa ccaacacttg aaaataacac tacatttttt ggttcaaaag taataaataa
    10981 tttagatgat tttaaaaata tcagtcacag tattattgct aatcgttacg atagtaatct
    11041 tgatgatgta aaagaaaaag tatataccag agatttattt caacgagatt aaaaaataac
    11101 acctcagatt tgtagtgtct gaggtgtttc tataaacttt tataagtaaa aaaatacaat
    11161 tatacttcat aaaaagactt ataccactgc gcaaatttac gaagcccgtc acgcaaactt
    11221 gtacttggtt taaattcaaa atctcgttct aacgcactag tatctgcata agtaactggc
    11281 acatctcctg gttgcattgg aactagttcg gtatgagatt caaagtcgta atcttctggt
    11341 agcacccctg ctgcaattag ctcttgttgt agaatatcta caaaatctag taggttttcc
    11401 ggcgtattgt taccaatatt ataaatatga tatggtggga ttggtagccc atcttcccca
    11461 tcaattttct ctggagcgca ctgcataacc ctctttactc cttcaacaat atcatctata
    11521 taggtaaagt cacgcttgca attcccataa ttgaatattt ggatattatc accagcacga
    11581 agtttatcag taaaaccaaa ataagccata tctggacgcc cagcaggacc atatactgta
    11641 aagaaacgta accctgtaga cggaatattg taaagtttag aatatgcatg tgccattgat
    11701 tcattacttt tcttagttgc tgcatacaac gatacttgat tgtctacttt atcctctgta
    11761 gaataaggaa ttttcttatt agtaccataa acggaagacg atgaggcata cactaaatgt
    11821 tccacaccag attctccatt atcatatgaa tgacgacaag cttcaagaat attatagaat
    11881 cctattatgt tagattcgat atacatatct ggattagtaa ttgaataacg gactcctgcc
    11941 tgagctgcta aattcaccac gatacttggt ttataattat caaagatgaa atcaattgtt
    12001 gctttatcag caatagaacc tttgataaaa ttccaagtag atcctggatg gttctttgcc
    12061 tccgtttcta tctctgataa tctatagttc ttaatagaga catcatagta atcattcata
    12121 ttgtcaatgc caatgatttt tattgatgac tgagtacgta ataactcaag aaccaaattt
    12181 gatccaataa aaccggctgc gccagttatt aatattgttc tatttgataa ggacgtgttt
    12241 aattccagca ttttttagca ctcactttct ttttttctta tgaatacata tttgttatat
    12301 attaaaaaat cttatttaga cttatctatt gtcactttta tcattctaat ctttgcacgt
    12361 agtagaataa ttatacagat aggaaattgc taagcaaccc acttctttct attatctatt
    12421 aacttattct aaaacgaata gaaattattc ttgatcacat aagtaacctt aaaagaaaaa
    12481 a
//
LOCUS       DADVXN010000083        12240 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.086, whole genome shotgun sequence.
ACCESSION   DADVXN010000083 DADVXN010000000
VERSION     DADVXN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.086"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(449..1174)
                     /locus_tag="KB942_002041"
     CDS             complement(449..1174)
                     /locus_tag="KB942_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852461.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1328..1654)
                     /locus_tag="KB942_002042"
     CDS             complement(1328..1654)
                     /locus_tag="KB942_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852462.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1691..2056)
                     /locus_tag="KB942_002043"
     CDS             complement(1691..2056)
                     /locus_tag="KB942_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBB6852463.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(2093..2308)
                     /locus_tag="KB942_002044"
     CDS             complement(2093..2308)
                     /locus_tag="KB942_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852464.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2437..2772
                     /locus_tag="KB942_002045"
     CDS             2437..2772
                     /locus_tag="KB942_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852465.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2848..4845)
                     /locus_tag="KB942_002046"
     CDS             complement(2848..4845)
                     /locus_tag="KB942_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852466.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4943..5218)
                     /locus_tag="KB942_002047"
     CDS             complement(4943..5218)
                     /locus_tag="KB942_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852467.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5450..6184
                     /locus_tag="KB942_002048"
     CDS             5450..6184
                     /locus_tag="KB942_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852468.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6223..7365
                     /locus_tag="KB942_002049"
     CDS             6223..7365
                     /locus_tag="KB942_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6852469.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7993..8478)
                     /locus_tag="KB942_002050"
     CDS             complement(7993..8478)
                     /locus_tag="KB942_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBB6852470.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8480..9244)
                     /locus_tag="KB942_002051"
                     /pseudo
     CDS             complement(8480..9244)
                     /locus_tag="KB942_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9414..10607)
                     /locus_tag="KB942_002052"
     CDS             complement(9414..10607)
                     /locus_tag="KB942_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6852471.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10691..10891)
                     /locus_tag="KB942_002053"
     CDS             complement(10691..10891)
                     /locus_tag="KB942_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6852472.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(11315..11548)
                     /locus_tag="KB942_002054"
     CDS             complement(11315..11548)
                     /locus_tag="KB942_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852473.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11555..11986)
                     /locus_tag="KB942_002055"
     CDS             complement(11555..11986)
                     /locus_tag="KB942_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6852474.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3770 a         2494 c         1967 g         4009 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
       61 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
      121 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
      181 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
      241 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
      301 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
      361 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
      421 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
      481 aatcagttac ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta
      541 accgaagtgg aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat
      601 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
      661 tgattccttt atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta
      721 caaatgaatt aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg
      781 tatatatggc ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat
      841 tagttagtaa agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc
      901 taacagcttt aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt
      961 ccataaaccc cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt
     1021 tcatttctga gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct
     1081 gattcgtttt taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg
     1141 ccaaacaaat ccataataat tttttctttt tcatttttcc tcctatacac tgctgttata
     1201 acagtgacta atgtaaagat tttcccagca atcatcagac tattatttac agggaatcaa
     1261 aaaaactcct tagtaagctt acacctttag gagttcctgt ttgaacggta tgcataatac
     1321 atttaaatca atttaacatt aacacctcag tatatggtat ctctacatat acacggtctt
     1381 tttttggata gtactcgaca tttatattga tagcacttcc attgtctaac gtagctttag
     1441 cccggtacgt gttatcacca ttatcatgag tgatcgtcac atcatcacat gtccgattta
     1501 agttatactg ttcttgtaaa atctgagtta ccaatggtac agatgacttc tctagtccag
     1561 atgaacccgt cattcttgaa aaaattatac ctataatagc caatacaacg atacctataa
     1621 tgacaaactt cttattcata tttactttat tcaacttttt atctcctatc tccaaaatga
     1681 ttttctctca tcagtaactc attgacatcc ccaatagtac accaaagatg gctccagcta
     1741 cccctgctat catcaaaatc gtacccattt gttcatcttt ctctcgatat aagtaaccca
     1801 taataacagc aacagcacca aataccacag gaataaatgc taatgatact accatagaaa
     1861 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
     1921 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
     1981 tttcttgtaa tgctccgcaa aactggcaat acttactttc atttgttatt tctttaccac
     2041 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agctaactgt
     2101 ttactttatt aaatacatca gaaaacagct catttattgg tacacttaat atctcagaaa
     2161 gcattttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
     2221 gctgtgtaac attcattttt ttggctaact cagtttggga aagtcctttt tcttttctgt
     2281 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     2341 atatgatgta tttcaactca taaaatatat aaatacttca aatgtcatta caaatacaac
     2401 aacttgttgt actctactga gaaggggggt gtattaatga aacagaagca atatgcaaag
     2461 gtcattagag aaaagcgaaa agcactcggg tggacacaaa aagaattagc taaaaaaatc
     2521 ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagaacc taacctcgat
     2581 agcttaacag ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt
     2641 gacatagatt atgaggaaga gtttttggct ctctatcgct ctcttagtac tgaagatgca
     2701 attcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta
     2761 ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa
     2821 taagattaag agctatggat aacctgtcta gtttaatata attgggtctc ttctattcct
     2881 tactctccgt ccatcatctc ttgttacact tgtaaattga taaataggaa ctaattcaac
     2941 tcctgataat atcaattctt ttattacttc atctatccct gcccctttcg ctagatatac
     3001 taaaaaacta ctgcttgctt tcacattgta tagtttctta atatccttag aagctcgttc
     3061 aagagtgaat ccttgtttaa caaagtagcc tctgttttct attaaagcat ttttcacgct
     3121 gttatccctg acatttcgta ggcgttcaca aacagtgtta ataagttctt tttctgtcat
     3181 tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa
     3241 ttgtttttcc ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt
     3301 ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt
     3361 ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac
     3421 gtctaatcga tagacattgc ttctcttatg tttaaagttt cctaacgctt tctcttcctt
     3481 taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat
     3541 caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt
     3601 accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact
     3661 ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat
     3721 ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc
     3781 aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt
     3841 ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac
     3901 tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc
     3961 agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa
     4021 gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc
     4081 attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc
     4141 tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct
     4201 ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt
     4261 aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa
     4321 aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta
     4381 ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat
     4441 cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt
     4501 atgaactaaa ttgatagata aaaccttttc tatgcttaag tatctactat catctttact
     4561 caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac
     4621 tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa
     4681 tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc
     4741 taaacaccta tctcttacat cagttttttc tttttttgta actggcttct ctaataattt
     4801 ttctttatct acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt
     4861 ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt tattactata
     4921 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
     4981 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
     5041 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
     5101 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
     5161 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
     5221 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
     5281 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
     5341 atcgacatac acataatacc tcgataaaaa aggggtgtca atagttttgt aaaatttatt
     5401 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
     5461 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
     5521 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
     5581 tgacaaaaag actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
     5641 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
     5701 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
     5761 tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt
     5821 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
     5881 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
     5941 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
     6001 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
     6061 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
     6121 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
     6181 ctagtggtaa gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag
     6241 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
     6301 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
     6361 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
     6421 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
     6481 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
     6541 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
     6601 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
     6661 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
     6721 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
     6781 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
     6841 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
     6901 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
     6961 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
     7021 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
     7081 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
     7141 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
     7201 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
     7261 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
     7321 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
     7381 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
     7441 tctagctcct ataacaggtt tacccaacac tgccttccaa tgactctttt gggaatcaga
     7501 ggaaagaatc aggctttata gagatatttc atctaacaaa ttactttaga ctgacagtat
     7561 atatcagcag gtactgtcat cgggaacgta gccaatatcg tatccaaaac agcaggtaga
     7621 gaagctattt gaggattcaa gttattctat ttcaaccatg aagttaagtt cattaaaaat
     7681 aaagatagtt ggtatttcaa taatttttgt atagagttaa tcactttatt gatgctaatt
     7741 catcttgatt ggtattataa tcgtattcaa gttgcgtggc gtgcttttgc tagaaaatat
     7801 ataactgttc tttataaaga aatataagga ttaaacattt tttatcatag agttttactt
     7861 atctatagaa ttatgtctgt tcctaattca gtttgatgaa ttaactgata aaaaaatatc
     7921 tactatttaa agatacaggg ataagtaagt aaaaaaataa catgtcatta gatagtagac
     7981 agttccaact ctttatttac cttttttgta tgctgcactg tcaatccaag ttcctaaatt
     8041 gttgtatcca tcatcactta tataatcata ttggccatca ataatttcgt caaaccacaa
     8101 atattctcca tctggttttt gcccaattat tttaattact gattgactag tagttgtcat
     8161 ctggttaaaa tcttttattt tacctatatt aacggccaaa atagcatttc ctcgcttata
     8221 actctcctgg atttcatatt taacatattc tcgatcaaga gtttctttac ctatcagcac
     8281 cacagtcact gatgttccaa ttaattgatt atctatccag ttttttatag caatatctcc
     8341 attacgttta actttctcaa attcagcctt atctacaaaa ccagcttcct ttgttcctcg
     8401 caaagtccag ctgtttctaa ctaccattgc tcttgttaca tctgcataat ggaaactaaa
     8461 aaaaaccctt ctcaccatat taatcctcct gaatactatt tattaaactg acaatttgtt
     8521 caactttttt atctacttct ccttcagtat ctaatttttc tagcgcttga aatttacttt
     8581 ttaaggaatc atcaaaataa tcactgaaat tctcttgaat cttatcaaac aaaaccttac
     8641 ttgcaccttt ggtaaatcca atcggaacta ctatattttc attttgaaca gataattcaa
     8701 attcttcaat cattccatct gctgtaacta tttcacctga ttcaagtttg tttccaaaaa
     8761 caaaaatact aatcccagac tctgcaataa tgttttctcg atatcttctc cactcatgag
     8821 gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg tatgttattt tttcttaaat
     8881 ttcttctctg ctttagaact cctgaaacaa tactattacc aattccaagt ccgtaacctg
     8941 atactattct ataaccggat tcagaaagcc tatatgctaa tttctcgaca aattttaatc
     9001 ctttcgattc agatgtaaat ggagtataat caacagcact tcccgaaata aaaatagttt
     9061 ttctcaaata cttttttcta atacttttca aaatatcagt aatctcagaa taatcatcta
     9121 cttcaacaac gtttatcgaa aatctggaca aatcattgat ttgtagtctt ctttttgtca
     9181 gttcataatt atatctctct tgatcaaaaa aatcctcaga atcgacaaaa ttttccttta
     9241 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     9301 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     9361 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     9421 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     9481 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     9541 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     9601 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     9661 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     9721 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     9781 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     9841 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     9901 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     9961 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
    10021 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
    10081 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
    10141 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
    10201 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
    10261 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
    10321 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
    10381 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
    10441 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
    10501 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
    10561 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
    10621 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
    10681 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
    10741 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
    10801 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
    10861 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
    10921 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
    10981 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
    11041 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
    11101 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
    11161 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
    11221 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
    11281 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
    11341 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
    11401 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
    11461 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
    11521 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
    11581 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
    11641 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
    11701 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
    11761 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
    11821 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
    11881 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
    11941 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
    12001 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
    12061 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
    12121 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
    12181 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DADVXN010000084        12130 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.087, whole genome shotgun sequence.
ACCESSION   DADVXN010000084 DADVXN010000000
VERSION     DADVXN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.087"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..563)
                     /locus_tag="KB942_002056"
     CDS             complement(<1..563)
                     /locus_tag="KB942_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBB6852475.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="KB942_002057"
     CDS             complement(750..1400)
                     /locus_tag="KB942_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852476.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="KB942_002058"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="KB942_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBB6852477.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="KB942_002059"
     CDS             complement(2912..3910)
                     /locus_tag="KB942_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBB6852478.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="KB942_002060"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="KB942_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBB6852479.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="KB942_002061"
     CDS             complement(4926..5657)
                     /locus_tag="KB942_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBB6852480.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="KB942_002062"
     CDS             complement(5661..6926)
                     /locus_tag="KB942_002062"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBB6852481.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="KB942_002063"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="KB942_002063"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBB6852482.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="KB942_002064"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="KB942_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBB6852483.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
BASE COUNT         3485 a         2686 c         1958 g         4001 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat
//
LOCUS       DADVXN010000085        12101 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.088, whole genome shotgun sequence.
ACCESSION   DADVXN010000085 DADVXN010000000
VERSION     DADVXN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.088"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..540)
                     /gene="frr"
                     /locus_tag="KB942_002065"
     CDS             complement(<1..540)
                     /gene="frr"
                     /locus_tag="KB942_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBB6852484.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEK"
     gene            complement(543..1265)
                     /locus_tag="KB942_002066"
     CDS             complement(543..1265)
                     /locus_tag="KB942_002066"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBB6852485.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1401..2282)
                     /locus_tag="KB942_002067"
     CDS             complement(1401..2282)
                     /locus_tag="KB942_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBB6852486.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2381..3163)
                     /gene="rpsB"
                     /locus_tag="KB942_002068"
     CDS             complement(2381..3163)
                     /gene="rpsB"
                     /locus_tag="KB942_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBB6852487.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3522..4001
                     /locus_tag="KB942_002069"
     CDS             3522..4001
                     /locus_tag="KB942_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBB6852488.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4218..5309
                     /gene="phnW"
                     /locus_tag="KB942_002070"
     CDS             4218..5309
                     /gene="phnW"
                     /locus_tag="KB942_002070"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBB6852489.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5302..5430
                     /locus_tag="KB942_002071"
     CDS             5302..5430
                     /locus_tag="KB942_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6852490.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5434..5979
                     /locus_tag="KB942_002072"
     CDS             5434..5979
                     /locus_tag="KB942_002072"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6852491.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6433..8124)
                     /locus_tag="KB942_002073"
     CDS             complement(6433..8124)
                     /locus_tag="KB942_002073"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBB6852492.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8544..9491)
                     /gene="arcC"
                     /locus_tag="KB942_002074"
     CDS             complement(8544..9491)
                     /gene="arcC"
                     /locus_tag="KB942_002074"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBB6852493.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9606..10625)
                     /gene="argF"
                     /locus_tag="KB942_002075"
     CDS             complement(9606..10625)
                     /gene="argF"
                     /locus_tag="KB942_002075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBB6852494.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10716..11945)
                     /gene="arcA"
                     /locus_tag="KB942_002076"
     CDS             complement(10716..11945)
                     /gene="arcA"
                     /locus_tag="KB942_002076"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBB6852495.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3303 a         2515 c         2192 g         4091 t
ORIGIN      
        1 tttttcttta tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg
       61 tacgtctttt tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt
      121 tttatattca tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc
      181 ttctttttta acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac
      241 taagcgaatg acatttccat cattggttgg actgattccg atatcacttg ccataatagc
      301 tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc
      361 tggaatttgg attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt
      421 aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg
      481 aagattttgt gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat
      541 ttttatttcc cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt
      601 ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt
      661 gcagttgaat ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca
      721 aattttactg catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt
      781 ttagccatca ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct
      841 gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa
      901 tgacgttctg ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt
      961 tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata
     1021 actgtagcta acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg
     1081 atttgtccgc gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg
     1141 tgaacttctt tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct
     1201 gcgtccccag ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg
     1261 accatgttca ttcctccaat attttctatt gccattttac catacttaga tgatttcgac
     1321 actctaagac tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc
     1381 ccgtctgtta ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta
     1441 tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg
     1501 ccttttgatg caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc
     1561 accaaagcga tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata
     1621 ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat
     1681 tcagcttctg gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata
     1741 gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata
     1801 cgtccgccca tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca
     1861 aagcgacgga agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca
     1921 attgtacctt tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca
     1981 gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag
     2041 tctgtttctg agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc
     2101 aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt
     2161 aaaagatcaa ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc
     2221 atcattccta cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc
     2281 atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag
     2341 gcttgtcgcc cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt
     2401 cgccttcaac aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat
     2461 cttcttcaac tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg
     2521 aagtaatcaa tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt
     2581 catctggatc gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt
     2641 cttgaacagc aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg
     2701 gcatatcagc gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag
     2761 caacttcttt tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt
     2821 tcaaacggct gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac
     2881 gatggttaac gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt
     2941 gtttttttgt acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat
     3001 cataagctgc atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc
     3061 tttctgtgaa gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt
     3121 gtacgccggc ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt
     3181 tggttttatt tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac
     3241 cgacaatcca ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag
     3301 acaaaagatc cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc
     3361 tgaaagataa aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt
     3421 tttccataaa ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt
     3481 tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac
     3541 atttgctgat taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa
     3601 acaaacttgg gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg
     3661 atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg
     3721 aaaaaggaac tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag
     3781 acattgtatt gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca
     3841 atgctcattt atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa
     3901 ttgctggatt cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag
     3961 aagaatttct tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa
     4021 tatggaatgc gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct
     4081 ttacaaaaat acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat
     4141 catcatttat attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag
     4201 aaatgaaagg aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa
     4261 cagtgaaaaa tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta
     4321 cacaatggat aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa
     4381 cggtcttaat gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca
     4441 ggccagaaga tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca
     4501 tgtgcgaatg tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc
     4561 aagctcatgt gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg
     4621 tccatagcga gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac
     4681 aagaaagaga tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt
     4741 gtgtagccga actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg
     4801 ttccaggctt ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta
     4861 gccgaagttt gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca
     4921 aatggcgttt cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac
     4981 tagaggcaga aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc
     5041 tacgacaaaa aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat
     5101 tcatcacctc ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt
     5161 atttaaagca aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc
     5221 gtatcggaaa tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca
     5281 aagactataa ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact
     5341 gtggactttg gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga
     5401 atccaactga ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc
     5461 caacagcttc tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta
     5521 cctcaggaaa aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat
     5581 ttggtcaagc attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac
     5641 gctgggatca atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca
     5701 gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa
     5761 gggtatggtc gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag
     5821 tcggttgatg aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat
     5881 gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat
     5941 gagtaccaaa aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat
     6001 tccaacaagc cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt
     6061 tgtacgctga catgaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg
     6121 tcctgtatca agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt
     6181 taggcttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag
     6241 ctgtgacacg acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa
     6301 cctctccact aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa
     6361 atttgaaaag ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac
     6421 attctctttt tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt
     6481 caataataca gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc
     6541 taaaatcttc gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt
     6601 cgcaatcact gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg
     6661 aactaattta accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga
     6721 agggacaaat cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata
     6781 aggtccagtt tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag
     6841 tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc
     6901 atctttgttt tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc
     6961 ttcatttaat acttcttcta ataagacgat ctttccttta cgagttgata attttttacc
     7021 accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat
     7081 ttccttcaag acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata
     7141 tagagattgt ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt
     7201 aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga
     7261 taggtcaacg atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt
     7321 gacgatttca tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac
     7381 ttccagcaga tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca
     7441 taattgaata gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc
     7501 atttaattct ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc
     7561 attgattggt tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag
     7621 ttttccaaac tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat
     7681 tttttccata atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga
     7741 gattggtttg gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat
     7801 gttgctatcc ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat
     7861 aagttcttta ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt
     7921 ggcagaatcg attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac
     7981 ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc
     8041 tagtaattgc tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt
     8101 tgcgacaaga tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa
     8161 caaaaaaaat ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg
     8221 cggtaccact ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg
     8281 tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac
     8341 cacttccgaa ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt
     8401 cttattaaac tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa
     8461 aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc
     8521 gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct
     8581 aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca
     8641 acaaatgcaa tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca
     8701 tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt
     8761 tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg
     8821 atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat
     8881 ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct
     8941 tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg
     9001 actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc
     9061 atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg
     9121 aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg
     9181 atcccttttt ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct
     9241 tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct
     9301 tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca
     9361 tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg
     9421 gcttttgcac ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt
     9481 cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg
     9541 agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt
     9601 tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt
     9661 gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca
     9721 tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg
     9781 tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt
     9841 ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat
     9901 ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca
     9961 cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct
    10021 gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc
    10081 gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg
    10141 cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca
    10201 gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag
    10261 aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac
    10321 atacgtccta gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca
    10381 tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa
    10441 cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga
    10501 tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt
    10561 aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct
    10621 ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac
    10681 ggcagctgac agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt
    10741 ggttggctca tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct
    10801 aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg
    10861 actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt
    10921 gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca
    10981 gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt
    11041 tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat
    11101 ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga
    11161 attttgattg ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat
    11221 ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt
    11281 aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg
    11341 attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca
    11401 cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa
    11461 gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct
    11521 gctgacagat catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc
    11581 atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct
    11641 cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg
    11701 acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct
    11761 tcattttgca atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga
    11821 atatcatcga acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca
    11881 ggccgtttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta
    11941 tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat
    12001 cactttcata attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt
    12061 aaataattaa acgtttttct atttttaagc gtaatttatt c
//
LOCUS       DADVXN010000086        11739 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.089, whole genome shotgun sequence.
ACCESSION   DADVXN010000086 DADVXN010000000
VERSION     DADVXN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.089"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..132)
                     /locus_tag="KB942_002077"
     CDS             complement(<1..132)
                     /locus_tag="KB942_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6852496.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="KB942_002078"
     CDS             542..841
                     /locus_tag="KB942_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852497.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="KB942_002079"
     CDS             914..2275
                     /locus_tag="KB942_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBB6852498.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="KB942_002080"
     CDS             2297..2557
                     /locus_tag="KB942_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852499.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="KB942_002081"
     CDS             2571..3323
                     /locus_tag="KB942_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBB6852500.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="KB942_002082"
     CDS             complement(3417..4022)
                     /locus_tag="KB942_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6852501.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="KB942_002083"
     CDS             complement(4075..5049)
                     /locus_tag="KB942_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBB6852502.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="KB942_002084"
     CDS             complement(5427..6146)
                     /locus_tag="KB942_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852503.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="KB942_002085"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="KB942_002085"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6852504.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVYEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="KB942_002086"
     CDS             7697..9094
                     /locus_tag="KB942_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6852505.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="KB942_002087"
     CDS             9175..9615
                     /locus_tag="KB942_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6852506.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="KB942_002088"
     CDS             9763..10089
                     /locus_tag="KB942_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6852507.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="KB942_002089"
     CDS             10090..10839
                     /locus_tag="KB942_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852508.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="KB942_002090"
     CDS             complement(10919..11374)
                     /locus_tag="KB942_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6852509.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3747 a         2055 c         2399 g         3538 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcatat acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaagcaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttat
//
LOCUS       DADVXN010000087        11693 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.090, whole genome shotgun sequence.
ACCESSION   DADVXN010000087 DADVXN010000000
VERSION     DADVXN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.090"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(105..629)
                     /locus_tag="KB942_002091"
     CDS             complement(105..629)
                     /locus_tag="KB942_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852510.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="KB942_002092"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="KB942_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBB6852511.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="KB942_002093"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="KB942_002093"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBB6852512.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="KB942_002094"
     CDS             complement(2351..3031)
                     /locus_tag="KB942_002094"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6852513.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="KB942_002095"
     CDS             3157..4026
                     /locus_tag="KB942_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6852514.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="KB942_002096"
     CDS             4052..4567
                     /locus_tag="KB942_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852515.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="KB942_002097"
     CDS             complement(4614..5207)
                     /locus_tag="KB942_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852516.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="KB942_002098"
     CDS             complement(5232..5411)
                     /locus_tag="KB942_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852517.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="KB942_002099"
     CDS             complement(5488..5997)
                     /locus_tag="KB942_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852518.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="KB942_002100"
     CDS             complement(6093..6473)
                     /locus_tag="KB942_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6852519.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="KB942_002101"
     CDS             6803..7552
                     /locus_tag="KB942_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBB6852520.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="KB942_002102"
     CDS             complement(7736..9013)
                     /locus_tag="KB942_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852521.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="KB942_002103"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="KB942_002103"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBB6852522.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="KB942_002104"
     CDS             complement(9935..10879)
                     /locus_tag="KB942_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBB6852523.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="KB942_002105"
     CDS             complement(10881..11567)
                     /locus_tag="KB942_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBB6852524.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3341 a         2472 c         2049 g         3831 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaa
//
LOCUS       DADVXN010000088        11572 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.091, whole genome shotgun sequence.
ACCESSION   DADVXN010000088 DADVXN010000000
VERSION     DADVXN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.091"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(135..494)
                     /locus_tag="KB942_002106"
     CDS             complement(135..494)
                     /locus_tag="KB942_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852525.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(466..837)
                     /locus_tag="KB942_002107"
     CDS             complement(466..837)
                     /locus_tag="KB942_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBB6852526.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(854..1156)
                     /locus_tag="KB942_002108"
     CDS             complement(854..1156)
                     /locus_tag="KB942_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852527.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(1173..1643)
                     /locus_tag="KB942_002109"
     CDS             complement(1173..1643)
                     /locus_tag="KB942_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBB6852528.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1618..1896)
                     /locus_tag="KB942_002110"
     CDS             complement(1618..1896)
                     /locus_tag="KB942_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBB6852529.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1893..2942)
                     /locus_tag="KB942_002111"
     CDS             complement(1893..2942)
                     /locus_tag="KB942_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBB6852530.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2899..3828)
                     /gene="tadA"
                     /locus_tag="KB942_002112"
     CDS             complement(2899..3828)
                     /gene="tadA"
                     /locus_tag="KB942_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBB6852531.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3960..5714
                     /gene="ade"
                     /locus_tag="KB942_002113"
     CDS             3960..5714
                     /gene="ade"
                     /locus_tag="KB942_002113"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBB6852532.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(5812..6474)
                     /gene="trmB"
                     /locus_tag="KB942_002114"
     CDS             complement(5812..6474)
                     /gene="trmB"
                     /locus_tag="KB942_002114"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBB6852533.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(6544..7326)
                     /locus_tag="KB942_002115"
     CDS             complement(6544..7326)
                     /locus_tag="KB942_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBB6852534.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(7530..8741)
                     /locus_tag="KB942_002116"
     CDS             complement(7530..8741)
                     /locus_tag="KB942_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852535.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(8734..9471)
                     /locus_tag="KB942_002117"
     CDS             complement(8734..9471)
                     /locus_tag="KB942_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852536.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9609..10043
                     /locus_tag="KB942_002118"
     CDS             9609..10043
                     /locus_tag="KB942_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBB6852537.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            10036..10380
                     /locus_tag="KB942_002119"
     CDS             10036..10380
                     /locus_tag="KB942_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6852538.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            10560..11570
                     /locus_tag="KB942_002120"
     CDS             10560..11570
                     /locus_tag="KB942_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6852539.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         3753 a         2335 c         1972 g         3512 t
ORIGIN      
        1 tcacctgttt aattctatcg aacaattgac aaaatttcaa tcgttttcac tgttttttat
       61 ttgtgaaacg gcaaacattt ttttattaaa aacagtgtta taacagaaaa gagggcgttt
      121 tcacgaacat tccatcattc tgtactagta tctgtatcat tttttacttc ttctgtgaac
      181 cgaaagataa acggtgagac ttccacctct aagaataggg actgttgtac ccactgatat
      241 gttacagatc cagttgtgta gtaaaaaact ccttccgaac tttcggcaag cgtagactgt
      301 tccgataaag ccatctgctt cattattttt gctatataat aatttttgat tcgtatatta
      361 agatccattg acaaacggta gctttctaat gctgccatca ataaaaacgt acataaaaga
      421 aataaaagca agacagaaaa taaaatagag cccttataac attttctact gatcgttttt
      481 tgtccatatg gcatatccat cttctccatt ttcaaagtgt acataaaaag aaattgtgtg
      541 ttctttttcc aataattgaa gctcagtgat acttgttaac ataggttgat gtccgccgga
      601 agttcttctt ttacgtatca ttgcctgata tctttcgatg atatactgtt tatcctcgat
      661 cgtgtagtgc agttgctgat ttttcacaga gacattgtga ctgctcttga gttcttcttc
      721 taattgtgct aaaaatatta gccactcttt ttgatcttta cgctgcaaaa attcacttac
      781 ttggatggtt tgctgaatca aaagattgaa tgtgagcaat agactgctca atatcatcag
      841 tccaattaga cattcaatca gcgtaaatgc tggaattact tgttttttca atactgatcg
      901 cttctttctc catattgaca aacacttttt cgattactcc attattatcg acaatttgga
      961 tatcgtatgg gatcatttct tcccttcgct ctggtctttt gtgatacatc cgctgcgtct
     1021 tgatatcttc ataaatcatt cgaataaata gcagttcctc cccacttttc ttttctgcct
     1081 gaagcaaaaa cagctggcca aagctcacta agtagacaca gacagaagcg atcataagcg
     1141 ctgtaaggga ctccaacaaa acaaatcctg ttttattcaa ttctttttgt atagcgacca
     1201 ctccctatct ggaattgata cgtaatcgtc cgctcttttt cttcccaata aaattgataa
     1261 gcttttagtg aactttcatt cccggttcta gcagcaaaag taatagaagc atggcgtttc
     1321 aaggtaatct catcaggaat atctaatact tcccaattta ttttatctgg attcggaaca
     1381 tgaaaaatga tttgattctc ttcactattc caataaaagc ctgtttggat ctgattcaca
     1441 atagcgattt tttgcgtaga atagattcgt ttttcaaatt ggccgaaaaa ttgatagact
     1501 gccaattcat tcttccatgc agaaaatgcg agaactggaa aagaaaggat cgttgtaagt
     1561 aataaaatca gtaagagtga ttcaaacaac gtatatcctg atgctaaaac cttaccttca
     1621 aggccgctga tagatttctg catgttctcg tttgatatag ccttcattca acaattcctc
     1681 cacagtagga actcttccat tattttgaag cgcatagagc tctttttgtg tgtctaccaa
     1741 ctggatgatc gcttcacgac tttcttggtc tacatgattc ctataacgcg acaaattagg
     1801 gacgaacaac aacactaata cactgatgat cagtaagacg attagcattt cgatcaatgt
     1861 aaaaccgcta taataagata tttttctctt tttcatggca aaacctcctc tatgcttccg
     1921 taaacaggta ataaaacagc ggcataaact aataaaatca aagccgctac gcccaaaaaa
     1981 atgattggct gaataaaatg catccagtga tgaagacgtt tgaaaagctg ttttcttgtc
     2041 cattcgctgt aaaaaatcag ttctttacct aaacttccct ttgcctctcc ctgtaaaaca
     2101 attttactga attcttctga tagaaaacga taattctgga actgctcagc caatgacata
     2161 cctgcctcca atccttgaat caacttagag gcaagatgct gaatcagact ttcttgtttc
     2221 gtttccttta agcagactat gatttgcctt aattcgaaac cttcatgaaa aagctttcca
     2281 atctctagtg acaaataagc cgattgatac aaggaatata tcggtccaat caataattgt
     2341 ctgcttatcc attcactctg atcaacagga gacaacttca tcagacgata ccttactagt
     2401 aaagccaata gagtaaccgc taggatgaat agtagaacat accaatgaaa gacttgtatc
     2461 agctgaatac cccaatgctc ttgcatgacc attcctgaac caataagctg aggcaaaacg
     2521 aacaaccgca tactaaacaa gatacctagc aaaaaggtga gcaatagcat cggatatgca
     2581 agcagctttt tcaaatcatt gcgaaatcct tcaacaagcc gaaactgttt ggcaatttcc
     2641 aacaaagttg tcggcagatt cccatgacgt tcagctaact cgatctgtac cagttgctct
     2701 gctccatatc ctatttgcga aagtacctca ctaaacgctt gcccttcctt tagctgatgt
     2761 tggatatttg ccaaaataac aggtggaaac gaacggatcg tgagtaataa ataaagggat
     2821 tcttgcagac taaaaccaga taaaagcagt tctcccatgg tttgtgcaaa ctggttttgc
     2881 tggcttcttg ttaatccttt agaatagttc tgactgccaa atattttcct gagcttcttc
     2941 ataactttcc tcccatgatt tttttactga ttcttcctcc ataaattgat aactccataa
     3001 aacagaagga tggtgattgg catcttccaa aagttcttga taaatgacac cagctaaaca
     3061 ttcagatagc tcttcttttt tctttgtcag ttcatggatt ctgaccaatg taccttcaag
     3121 attgcgtgca tgtacggttg caaaaacccg gtgccctgtt agtgctgctc ttaccgcagc
     3181 atgtgcagtt gattcatctc ttatctcgcc aatgattagc aagtctggac gatgccgtaa
     3241 tgcgagtctc aaaagtgtat cataagtctg tgctattttc ggattgatct gtagctgcag
     3301 aaaaaacggt tcttctatct ctacaggatc ttcgatagta atcacttgca atttttcttc
     3361 caaagccaac cgatacatta gagaagtctt gcctgatccg actggtccgc tgaaaaggta
     3421 taatccacgt ctgacggttt tctgtaaaat agttgcaaaa tctcgagaac taaagcaacg
     3481 atggggtgtt tcaaaagtac cgtataatag ccgaatcact aaactctctc gttgaaggta
     3541 atctcctaca gaagacaacc gcagacgaac ttcctgtttt tctaaagaat aagtaaatgc
     3601 acctaactga gcttttcttt tttctccgat atccatctgt cccaaatact taaatcttga
     3661 gatcagttgc tgtccatctg ccgaagagac tttcttccag aatgttcgtt tttttccata
     3721 gcgaaaatag atgtaaaata cttctgaagc tggcaatata tatacgtctt gggcatgttt
     3781 ggcaaaactt tcacttatca tttgatccga taatcgtttt atttccatta ttctcccctc
     3841 cacaaaaaac atacgtcttt ttgatctttt tgtttcaaat aaaatgaatt tacaaaaaaa
     3901 tattggcaaa aaccgaattt atgttatact tataaaaatt tatcttggga ggcactccat
     3961 tggaaacaac attaaggaaa tatatcgaag tagcaagtaa aaaaaagcct gctgacctag
     4021 tgatcaagca tgctaagatc gtcaacgtct ttacgaaaga aatcatggaa aaagatgtag
     4081 caatctgcga aggaatgatc gtaggaatcg gtgaatacga aggaaaaagc gtacataatg
     4141 ctaatgggca atatctagtg ccaggattca tcgatggcca tgtgcatatc gaaagctcac
     4201 ttttatcacc aagcgaattt tcaaaagtat ccttgcttca tggagttact actgtagtga
     4261 cagatcccca tgaaatagga aacattgctg ggagtacagg tcttgagttt atgatagaag
     4321 atgcaagaca aacactcatg aatatctttt ttatgctacc ttcttgtgta cctgtaaccc
     4381 cttttgagac caatggtgca aatttaaatg caaaaaaatt agcccctttt ttacaaaaac
     4441 ccgaagtttt aggcttagct gaagtcatga actatcaagc agtagcaaat aatgaaacag
     4501 acatcctaga aaagatccaa ttgatgcacc agcataagaa aaaaattgac gggcatgcag
     4561 ctggtatcgg catggaagag ttaaatgttt atcctgcagc aggtattcgg acggatcatg
     4621 aagctacaac tgcaaaagaa gctaaagaac gtttggatct tggtatgtat ttaatggttc
     4681 gagaagggac cgttgcaaaa gatctggcat cgctactacc ggcgatcaca cctgaaaatt
     4741 caagaagatg cttctttgtc accgatgata aactgatcaa tgatttagta aaagaaggat
     4801 caatcgatca tatcgtccgt caagctattg cacttggaat cgatccatta caagccattc
     4861 agatggcaac attgaatgca gcagaatgtt ttgatttgaa aacactaggt gcgattgccc
     4921 ctgggtatca agctgatttc ttcttaacag acgatcttac caagctgcca attcatgaag
     4981 ttttctataa gggaacatta gttgttcaat ctgggcaact ccaagaccat ttatttgctg
     5041 atcattcaaa ttcatatacg tgttgtctgc caaaactaaa cgctcaacca ttaaatccac
     5101 attcgttaga gctgcttctt tcttcttcac aagcccatgt gatcgaaatt atcccgaata
     5161 gtctagtgac taaagatttg gtagaaaaag tggaccgaca aggcgaacga ttcattcctt
     5221 caatcgaaaa agaccaatta aagattgcgg tgatcgagcg tcaccatcaa acgggaaaca
     5281 ttggattagg catagtgaaa ggatttcagc tgaaacaagg agcgatagcg acaacgattg
     5341 cccatgactc gcataatcta gttgtcgttg ggacaaacga cgaggatatg ctgtatgctg
     5401 ctaatctttt gattcaaaaa ggtggcggga tgaccgttgt caacaatcag caggaaatag
     5461 cttgtgtttc tcttcctata ggcggaatca tgacacagga acctttttcg atcgtctatg
     5521 aacagttgaa tgaattaata actaaagcac atcaactagg agcagctaaa acctttgatc
     5581 cttttttgac actttctttc ttaacattat ctgtcatacc cgaattgaag atcaccgaca
     5641 aaggattatt ttcattttct cgcttccaat tgattccacc ttgtggaaaa accgatgaaa
     5701 taaatacatt ttaaatcaaa taaatcccag aaaatacatt acatctgtaa tgttcatttt
     5761 ctgggattta tttatggata ttaatgtttt tatgaaagca gaaagttagt ttcacagact
     5821 tttctttttg gtcttatctt gaaattgagc ttctactcga taaatacgtt gaccttttga
     5881 agaaaatttt tcttcatact ccgtcataat attgccttca aattgacttt gatgtaaatc
     5941 taaccatacc tgtttgagga tcatgccata ttgagaaaaa cttgccagag aatactcaaa
     6001 caatccttga ttatctgttt tgaaatggat ttctccattt ggacgaagaa tctgttcatc
     6061 tacagcaaga aaagtcttat atgtcaatcg ccgtttttca tgacgttttt ttggccatgg
     6121 atcggaaaaa ttcagataga tttgatctac ttcgctgtca gcgaaatact ctgtcaacgc
     6181 tgatccatcc acatgtagaa gtttcaggtt aggaagcggt tgttcgatca acttgtctag
     6241 tgcaatcgaa acaacactga cctgcatttc tactccaata taattgattt caggatgggc
     6301 ttttgccatt ccagtgatga actggccttt ccccatacca atctcaatgt ggattggatg
     6361 atcgttttca aacaattcat tccattttcc cttccacaat gttggatcac taattacaaa
     6421 attcgggtga gccgctaaca tttccgctgc tcccggacga tttcggacac gcattattta
     6481 tctccattcc tttcctctaa ttaaaaaggt ctggccaaat ctccgcagag tttgggccaa
     6541 acctcagcca ctgaaaattc gttttaattg taaaatttct tcgttcatcc gtcgatgatc
     6601 ttcacgtttg caatatctgg tgatttcttg gagcaaattc atggccgcat accaatgaaa
     6661 tttttcaagc atttcgtcag tcgggcgtat gccataactg atcagccact gattccagct
     6721 agacagtggc acataatttc ctaaaatcgt tgcaatatcc accgcagggt ctgcaaacat
     6781 gattgaatcc caatcgacta gatataaata atttttacat actaaccaat tacgatggtt
     6841 tacatctccg tgtactactg ataagttttc cttatctatt gacgggatct gctcacgaag
     6901 aaaacgatgg acaatttgca gaaattgatt tttctggagt tcttccggta attctttttc
     6961 atatttttgc agcatcttct ccggcgtcat gacacgacca ccgatacggg tcagcatggt
     7021 tttaagagaa tttgaatgat gtaaatggta caaaacatca atcacgtcgt ttctttgacc
     7081 aatctcatta ggagtcagca agcgaccttc cagccactct tgtgctgtca aagtatcgcc
     7141 attccccgtt cgttttgtcc aaacaagttt tggtgtaatt ccttcccttg aaagagcagc
     7201 caacatcgga gtcgtgtttc gtttaataaa gactttgtcc tcatcacgca tgcctatgta
     7261 tgtttttcct gtgtcgcctt tgatgggacg taaccgccac tcttggtcta actgaaactc
     7321 catcatgcat tctcccgtcc tcttactctt tgttttgcag tacttattat tctagtcgcc
     7381 tgaacactac acgtcaagca catatacgtt ctttttcatt taattgccat tcaaaaaacc
     7441 agcttttctc aaaaaaaata gaacgaacag cattcttact gtcaacgctc tatttttcat
     7501 tctcattttt accctacaca gctttgtttt cagtctgaca tcttcttcaa acgagttggt
     7561 acatatagtt taacaaagag catagtaaca atcaaataag ccgctagcgg aacccatcgc
     7621 tgctgcccat ctaatacgac tgcagcaatc aaaccaaaaa taaccgacgc gataattaat
     7681 acccatgtga gtagatactg aatcgcctgc ttcttctgat tcttatggat tggataaagc
     7741 tgaacgagaa ccatatagcg gaactggttc gctaaaggta acagttggaa accgatcaaa
     7801 tataaaaata agaaaccgat aatcagacta aaataaaagt cgactactcc ggcaatcaat
     7861 aacccaccta taattactaa tcggaaataa agaccactaa aatcagaacc tcttgccaat
     7921 cttcttgcaa acaagtaaag ataagtgttt tttgattctc ttttaatatg agtcaatata
     7981 ggatccaaat agcgtcttct tttgatcttt gccgtaattt caggaacatc tgtgaataaa
     8041 ttgataaatt gatagatctg atgcaaccta tgttcttcta catgcaccat tttctcccaa
     8101 tccaatcctt tttcttgttg cttccacaaa aaccagtaat atagacttgc ctgtactaat
     8161 gcacctgcta ttcctgccca tattagccaa taaaatgata gaaacaaaaa gatcagagaa
     8221 gaaaaaaacc agcctaattt ccaataacga ataacttgtt gatctagtcg aaacaattct
     8281 gctctcttga tttgcagatg actgaatttc aaactccata atatgatcag atagacgaaa
     8341 aacatcgaaa aggattgctc cgtactaacc accactaatg gcatggccaa tccgcatgcc
     8401 aatatcaaaa agatcaacgg aaaaatgcaa gaatagcgaa aagctcgttc caaataggtt
     8461 tgcatttctt tttctttagg aagtaaaaaa acaatatctg ctgcctctgc aaaagtagcc
     8521 atcttaccca cgaataaaca gagcatccaa aatacagaga ttatgagccc tccaattgca
     8581 aacggtggct gcagggtttt caaccaatta gaataatata atcccacacc gccaatcaag
     8641 aaaagacaaa ctaaaacaaa atgatcgttc aatacataac gcatatattt catcattttc
     8701 ttttgatgtc ttgctaaacg tttttgaaaa aaactactca tgtgcctgct cctttgttaa
     8761 agcaatatag atttcgtcca aagaagaatc cggcagctga aattcttttc tcaacatctc
     8821 aagagaaccg tctgctcgta tctctccgtt atgtaagacc acaaaacgat cacaatattt
     8881 ttctgcagta gctaaaatat gcgtagacat caaaataccg gctccttctt ttttcatttc
     8941 taccatcaat tcaagcaatg cattgatagc tagtggatcc aagcctaaaa atggttcgtc
     9001 gatgatatat aaagaaggct cgatcaaaaa ggcgcataag atcatcactt tctgtttcat
     9061 tccctttgaa aaattagctg ggaaccagtc aagacgatta gataaccgaa aagtctccaa
     9121 caacttttct gctcttttca ttgctatatc cataggtata tcgtaggcca tcgcagtgac
     9181 ttcgatatgt tctctcaatg tcaattcttc atataaagaa ggagtctctg gaatataccc
     9241 aatttgtttg cgatattctt ccgtattttt ctttaaggat agatgattga tttctatcgt
     9301 tccttttgaa gcttccagca gaccgataat gtgcttgatg gtcgtacttt tccctgctcc
     9361 gttcagtcca atcaacccta ctatctctcc aggctgtaca tcgaaactga tatcttttaa
     9421 cacagggaga tgtccgtacc cccctgtgac atgatcgata attaaactca ttctttttcc
     9481 tccgccattt atttttattt attataccac gaagaaaatg acggtatttt gtttttttcc
     9541 ttacaatagc tttaacttat ggtaaagtta agctgaagta tttattcttt atagaaagga
     9601 tgttttatat ggaaaactgt attttttgca aaatcatcaa tcaagaaatc ccaagctata
     9661 aaatatatga agacgacaag gtctatgctt ttttagacat ctctcaagca acgaaaggac
     9721 acacattagt tgtgcctaaa cgtcatgtag cagatatttt tgaatatgat cccgaacttg
     9781 caggagaggt tttttcgcgt gtgcctaaga ttgctcaagc attggaaaaa gcttttccag
     9841 aaatgaaagg tcttaatatc ttgaataaca accgtgaact agcctatcaa tctgtttttc
     9901 attcacatat ccatttagtc cctcgctaca gcaaagagga tgatttttct attcactttg
     9961 tcaatcatca agatagttat ggatcggaag aactaaaagc tattcaagaa acaatcgtaa
    10021 agcaggtgtc ctgcgatgat taaaaacttt ttcaaaggac tgttatttgg agctgctgct
    10081 ggcactgtcg gaggtctgtt attggcgccc aggagcggga atgaaacaag aaagaaactg
    10141 atcgaagaat tggaagaagc aacaactctg acaaatgatt taaataacag cctgaatcat
    10201 tttaaaagcg cattgatcga aacaaaagaa acagctgaat ctgtgattcc tgctttccaa
    10261 gaagcaatca aaaaagatat cgaagatttt aaattccaag ccgaaccacg tatcacacaa
    10321 atcaatgaac aagtagaaaa actaaaagct gatctgccag agtatcctga acaaatataa
    10381 taatgattat tttctcaaca gatttaacta tataagtgag aaaagtttgc gtatttccta
    10441 ggaatgtgat gaaattttga attttgtttg aaactaccac gacaacgttt ttaaaggaaa
    10501 tgggatatgg tatagttaat atataaaaat aataactaat tacaggagtg caaattttaa
    10561 tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt ggctgtttta actctagcag
    10621 cttgttcagg cgatactaat aaagatatcg caacgatgaa aggcggaacg atcactgtat
    10681 ctgatttcta tgatgaagca aaacttgaat catcgaacca atccttagta caacggatga
    10741 tcatttataa agtattcaat aacaaatatg gcgataaagt aaccgataag caagtggatg
    10801 cagaatacga caaacaagcg aaatcattag gtgatacttt tgaatcacaa ttagaagctg
    10861 ctggatatac gaaagatact tataaagaat atattcgtaa caatttagct tttgaagctg
    10921 gcttgaaagc tcacgtagat attacagatg acgatttgaa aactgcttgg aaatctttcc
    10981 atccagaagt agaagcacaa atcatcaaat tatcaagtga agatgaagca aaagatgtga
    11041 aaaaatcagc ggatgatgga gatgatttct ctaaattagc caaagacaaa tcaactgata
    11101 cagagacaaa agaagatggc ggtaaagtta aattcgactc aacgacaaca actattcctg
    11161 cagaagtaaa agaagcagct ttcaaattga aagacggtga gatctccgat gttattacta
    11221 caacaaatcc tacttcttat gcgacagaat actacgttgt caaaatggtg aaaaaccaaa
    11281 ataaaggcaa tgacatggat aaatacaaag atcaactaaa agatatcgct actgaaacta
    11341 aactaagtga caatgcattc acaactaaag taatcggtga agaattaaaa gatgcaaatg
    11401 taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc attcacaaca acttcatcaa
    11461 gtacgaaaga ctcaagtgaa actacagctt caacaaagag ttctgacaca aaatcaactg
    11521 attcaacaaa agaatcttca actgaagaaa caactgattc atcaaaataa aa
//
LOCUS       DADVXN010000089        11506 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.092, whole genome shotgun sequence.
ACCESSION   DADVXN010000089 DADVXN010000000
VERSION     DADVXN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.092"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(218..703)
                     /locus_tag="KB942_002121"
     CDS             complement(218..703)
                     /locus_tag="KB942_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBB6852540.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    793..1138
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1183..1824
                     /locus_tag="KB942_002122"
     CDS             1183..1824
                     /locus_tag="KB942_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6852541.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1843..2478
                     /locus_tag="KB942_002123"
     CDS             1843..2478
                     /locus_tag="KB942_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6852542.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2496..3311
                     /locus_tag="KB942_002124"
     CDS             2496..3311
                     /locus_tag="KB942_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6852543.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3630..5066
                     /locus_tag="KB942_002125"
     CDS             3630..5066
                     /locus_tag="KB942_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6852544.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5255..7687
                     /locus_tag="KB942_002126"
     CDS             5255..7687
                     /locus_tag="KB942_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB6852545.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7917..8984
                     /gene="rlmN"
                     /locus_tag="KB942_002127"
     CDS             7917..8984
                     /gene="rlmN"
                     /locus_tag="KB942_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6852546.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9177..10280)
                     /locus_tag="KB942_002128"
     CDS             complement(9177..10280)
                     /locus_tag="KB942_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6852547.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10832..11380
                     /gene="aac(6')"
                     /locus_tag="KB942_002129"
     CDS             10832..11380
                     /gene="aac(6')"
                     /locus_tag="KB942_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6852548.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3818 a         1944 c         2480 g         3264 t
ORIGIN      
        1 gatttataat cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa
       61 atatggattt ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt
      121 gttacattgt ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa
      181 gacgcttgaa ttgggaaata agaaaactac tctatcatta caaattttca taaactggca
      241 gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg
      301 tattgatatt tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga
      361 cttggatttt tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa
      421 cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt
      481 gttcagctag ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt
      541 gttttccttt tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag
      601 ggacattttt gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt
      661 tttttagtag ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt
      721 tttttattag tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac
      781 gtaaagttaa aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc
      841 tctggttggt ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg
      901 aagcgtatag taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca
      961 gataaggctg gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt
     1021 cctttgatct tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag
     1081 gttttttctg caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc
     1141 atttgccaag aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa
     1201 gttatgccgc aattattagt aggagcactc acaacattga aattattttt atttaccttg
     1261 attggctcat taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg
     1321 ctacgctact tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa
     1381 ctgattttta ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca
     1441 gcactcattg cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg
     1501 atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt
     1561 caaacggtga cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg
     1621 aatgaagtta tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac
     1681 gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc
     1741 gtggcaatca tctatcttac attgactggt attctaacaa tcgtaatgaa acaagttgaa
     1801 ggaaaattaa attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa
     1861 gtgaacaaag catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat
     1921 gggaaaacat tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc
     1981 ttggctggct tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac
     2041 ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta
     2101 tttccacatc tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag
     2161 acaaaagaag aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat
     2221 ttattgaata tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc
     2281 agagcattgg ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat
     2341 cctgaattac gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc
     2401 caaattattg tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa
     2461 gtttccccaa ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg
     2521 ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata
     2581 attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc
     2641 ctatgggatt tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag
     2701 cagtctttga acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg
     2761 aatttgaact gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg
     2821 caagagaaaa gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca
     2881 cacctaaaga atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc
     2941 aaaatgggtc atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag
     3001 tcaaaaacca agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa
     3061 gcagacgggt cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa
     3121 gaaacgaact agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag
     3181 ttggtgcacg caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac
     3241 tagagaaaac aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc
     3301 cgagaaatta acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat
     3361 aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc
     3421 ttggtaaacg gtgttcaaaa gccagttttt cggtattaag ccaaatttat gcaaaaatgt
     3481 agaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
     3541 tcctttacat aattttattt tgttgtggaa ggggtttcgt gttattctaa aagaggaacg
     3601 attgaaggtt ataggaaagg aagagatgaa tggaacacaa agaacttacc tcatttccaa
     3661 aagattttct gtggggctct gcttctgcag cgtatcaagt agaaggagct tggcaagaag
     3721 atgggaaagg agaatcagtc tgggatcgat ttgtacggat acctggaaag acatttaaag
     3781 gaacgaacgg tgatttggca gtagaccact atcatcgtta taaagaagat attgctttaa
     3841 tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc ttggacacgg atttttccta
     3901 atggacgagg agaagtcaat caagctgggc tcgcttttta tgagagattg atcgacgaat
     3961 tgattgaaaa cgatattgaa cctatgttga ctttatatca ttgggatctt ccacaagcat
     4021 tgcaagatga atataatggt tgggaatcac gtcagatcat tgaagatttc actcattatg
     4081 cagaaacatt atttgaggca ttccgcggga aagtccatta ctggattagt ttgaatgaac
     4141 aaaatatctt cacttcatta ggttatttgt tagcagcaca tccgccaggt gtgacagacc
     4201 cgaaacgaat gtatgaagtt aaccatattg ctaatttagc aaatgcatct gtcatcaata
     4261 aatttcacga gttagaaatg cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt
     4321 atccaattga cagtaatcct gaaaatgttc tggctgctga aaatgcagaa gacctgatgg
     4381 ccaattattg gatggatgtc tatatgtggg gaaaatatcc gattgctgcc atgaggttct
     4441 tagaagaaaa aggatgggca ccaacgattg aagctggcga tgcagaattg ctagaatctg
     4501 ctaaaccaga ttttcttggg atcaattact atcaaacggc gacgaatgca ttcaatccat
     4561 taaacggagt tggtgctgga aaaatgaata cgacaggaaa aaaaagttca tcagaagaaa
     4621 ctggagttcc tggaatgtat aaaaaagttg agaatccatt tgtcgaacga acaaattggg
     4681 actgggagat tgacccagaa ggattaagga tcggactgcg tcgtatcaca agccgttacc
     4741 gcatgccggt actgattacg gaaaatggct taggagagta tgacaagttg acagaagata
     4801 aacaaatcca tgacgactat cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag
     4861 aagcaatttc ggatggagca gaagtcctag gctattgcac ctggtcttat acagatttgc
     4921 ttagctggct gaacggttat caaaagcgat atggatttgt atatgttgat caagacgaaa
     4981 cacaagaagg atctttggaa cgatataaga aagacagtta ttactggtac caaaaactga
     5041 ttgaagaaaa tggagagaac ctttagttca catacttaaa acaagaaggt acggctggat
     5101 atggccgtgc cttttcttga taaaatcatt tagaaattaa gaaaaactgt aaaataagct
     5161 gaaaaatgaa agaaagcgtc agttctttca aacaaaactt tagttccctt actttttttt
     5221 gtataatgaa acttgatgaa atgagggata ataaatggca caaaagaaac ggcctactaa
     5281 aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac
     5341 gattggtgtt gggtttgcct tgattttatt tgctgtcttc ggctttttaa aattaggttt
     5401 tttaggaata ttgattgcaa acggctttcg gatcattgca ggaaatactt accaaatcct
     5461 ctgtctttta ttagccgtat taggattttg gattgtcatt aagaatacag agttttcaat
     5521 tggtaaaaac cgacgctggt ttggcggcat actcttttat ttcggtatct tattattgtt
     5581 acatgcccat ttatttggca aactccatac tggggaacca aatatcatgg ggacgacatg
     5641 ggatttgtta gccagtgata taaaacaaag tcaagtagac aataacgttg gtggtgggat
     5701 gattggagca atcctttatc attttactta cttcttgatt gcacagcctg gaagttattt
     5761 agcagcagtg ctattactgg caggaggagc attcctgttc agtaatctgg aaggctatca
     5821 gctgttaaat ggtatccaat caattggaga acgagtacag gaattattgg aaggtgatcc
     5881 tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc
     5941 gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa tatgagaaga aaaaagcggt
     6001 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     6061 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     6121 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     6181 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     6241 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     6301 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     6361 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     6421 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     6481 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     6541 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     6601 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     6661 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     6721 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     6781 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     6841 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     6901 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     6961 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     7021 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     7081 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     7141 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     7201 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     7261 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     7321 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     7381 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     7441 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     7501 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     7561 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     7621 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     7681 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
     7741 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
     7801 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
     7861 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
     7921 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
     7981 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
     8041 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
     8101 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
     8161 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
     8221 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
     8281 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
     8341 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
     8401 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
     8461 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
     8521 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
     8581 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
     8641 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
     8701 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
     8761 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
     8821 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
     8881 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
     8941 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
     9001 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
     9061 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
     9121 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
     9181 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
     9241 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
     9301 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
     9361 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
     9421 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
     9481 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
     9541 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
     9601 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
     9661 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
     9721 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
     9781 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
     9841 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
     9901 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
     9961 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    10021 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    10081 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    10141 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    10201 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    10261 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    10321 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    10381 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    10441 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    10501 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    10561 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    10621 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    10681 aataaagtgt ttctgtataa ttttttattt tataagtaag ataaataatg aaagaataga
    10741 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
    10801 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
    10861 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
    10921 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
    10981 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
    11041 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
    11101 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
    11161 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
    11221 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
    11281 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
    11341 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
    11401 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
    11461 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtac
//
LOCUS       DADVXN010000090        11472 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.093, whole genome shotgun sequence.
ACCESSION   DADVXN010000090 DADVXN010000000
VERSION     DADVXN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.093"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            276..482
                     /locus_tag="KB942_002130"
     CDS             276..482
                     /locus_tag="KB942_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBB6852549.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(684..1685)
                     /locus_tag="KB942_002131"
     CDS             complement(684..1685)
                     /locus_tag="KB942_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6852550.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1690..3603)
                     /locus_tag="KB942_002132"
     CDS             complement(1690..3603)
                     /locus_tag="KB942_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6852551.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3771..4277)
                     /locus_tag="KB942_002133"
     CDS             complement(3771..4277)
                     /locus_tag="KB942_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6852552.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4437..4877
                     /locus_tag="KB942_002134"
     CDS             4437..4877
                     /locus_tag="KB942_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6852553.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4903..6060)
                     /locus_tag="KB942_002135"
     CDS             complement(4903..6060)
                     /locus_tag="KB942_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852554.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6063..6419)
                     /locus_tag="KB942_002136"
     CDS             complement(6063..6419)
                     /locus_tag="KB942_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852555.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="KB942_002137"
     CDS             complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="KB942_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBB6852556.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7887..8726)
                     /locus_tag="KB942_002138"
     CDS             complement(7887..8726)
                     /locus_tag="KB942_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6852557.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8911..9798
                     /locus_tag="KB942_002139"
     CDS             8911..9798
                     /locus_tag="KB942_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6852558.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10392..11086)
                     /locus_tag="KB942_002140"
                     /pseudo
     CDS             complement(10392..11086)
                     /locus_tag="KB942_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11070..11468)
                     /locus_tag="KB942_002141"
     CDS             complement(11070..11468)
                     /locus_tag="KB942_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6852559.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3422 a         2292 c         1718 g         4040 t
ORIGIN      
        1 aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat
       61 cttttctatt atattcccat attttctatt attggtctag acgtatgatg gtaaactttg
      121 tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg
      181 ctagatgatc attttattca catcctaaca aacttgtcac atttatagat tcttaatcta
      241 tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc
      301 tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca
      361 ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc
      421 ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct
      481 gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc
      541 gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa
      601 ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac
      661 aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt
      721 tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc
      781 tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct
      841 tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa
      901 cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta
      961 ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact
     1021 tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg
     1081 ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac
     1141 aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa
     1201 tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact
     1261 ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc
     1321 gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat
     1381 gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag
     1441 atatccatat tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt
     1501 tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg
     1561 tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat
     1621 acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat
     1681 ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa
     1741 aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct
     1801 tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc
     1861 acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc
     1921 ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt
     1981 ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt
     2041 cttttgctct gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc
     2101 tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat catggaacac
     2161 tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt
     2221 tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata actcatataa
     2281 tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg
     2341 caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg
     2401 atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat
     2461 gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt
     2521 aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt
     2581 gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata
     2641 aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc
     2701 atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga
     2761 atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat
     2821 cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg
     2881 atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg
     2941 atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc
     3001 tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag
     3061 ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag
     3121 ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat
     3181 ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt
     3241 accgatatca tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc
     3301 gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt
     3361 cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt
     3421 ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc
     3481 ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt
     3541 acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt
     3601 cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa
     3661 ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc
     3721 agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc
     3781 aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct
     3841 ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct
     3901 aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat
     3961 tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa
     4021 ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc
     4081 tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt
     4141 ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat
     4201 tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg
     4261 tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt
     4321 atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact
     4381 tttcgactat ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga
     4441 tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta
     4501 tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg
     4561 aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt
     4621 ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata
     4681 ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa
     4741 aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa
     4801 agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc
     4861 tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa
     4921 tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg
     4981 cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc
     5041 atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga
     5101 tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg cccataattc
     5161 ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa
     5221 aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt
     5281 attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat
     5341 cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt
     5401 cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata
     5461 atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga
     5521 cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc
     5581 caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat
     5641 gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc
     5701 ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga
     5761 gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc
     5821 tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt
     5881 tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa
     5941 aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga
     6001 taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa
     6061 acttatataa ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa
     6121 tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa
     6181 aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat
     6241 tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg
     6301 gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa
     6361 caatagttca aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa
     6421 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
     6481 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
     6541 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
     6601 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
     6661 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
     6721 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
     6781 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
     6841 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
     6901 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
     6961 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
     7021 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
     7081 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     7141 tcgctgctcg tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg
     7201 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
     7261 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
     7321 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
     7381 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     7441 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     7501 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     7561 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     7621 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     7681 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     7741 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     7801 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     7861 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     7921 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     7981 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     8041 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     8101 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     8161 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     8221 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     8281 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     8341 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     8401 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     8461 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     8521 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     8581 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     8641 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat
     8701 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
     8761 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
     8821 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
     8881 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
     8941 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
     9001 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
     9061 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
     9121 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
     9181 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
     9241 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
     9301 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
     9361 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
     9421 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
     9481 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
     9541 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
     9601 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa
     9661 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
     9721 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
     9781 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
     9841 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg
     9901 gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat
     9961 ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat
    10021 gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc
    10081 gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt
    10141 ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg
    10201 aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat
    10261 acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat
    10321 gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg
    10381 gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg
    10441 aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct
    10501 aatacttgat ctagtagctc tttacctaac gttatcgctg agctccagat cgaatggctg
    10561 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10621 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10681 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10741 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10801 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10861 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    10921 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    10981 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    11041 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    11101 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11161 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11221 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11281 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11341 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11401 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11461 atcgtcatct ct
//
LOCUS       DADVXN010000091        11387 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.094, whole genome shotgun sequence.
ACCESSION   DADVXN010000091 DADVXN010000000
VERSION     DADVXN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.094"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(24..476)
                     /locus_tag="KB942_002142"
     CDS             complement(24..476)
                     /locus_tag="KB942_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBB6852560.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            626..1576
                     /locus_tag="KB942_002143"
     CDS             626..1576
                     /locus_tag="KB942_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6852561.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1569..2528
                     /locus_tag="KB942_002144"
     CDS             1569..2528
                     /locus_tag="KB942_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6852562.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2525..3280
                     /locus_tag="KB942_002145"
     CDS             2525..3280
                     /locus_tag="KB942_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6852563.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3303..4256
                     /locus_tag="KB942_002146"
     CDS             3303..4256
                     /locus_tag="KB942_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6852564.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4900..5196)
                     /locus_tag="KB942_002147"
     CDS             complement(4900..5196)
                     /locus_tag="KB942_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBB6852565.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5551..5907
                     /locus_tag="KB942_002148"
     CDS             5551..5907
                     /locus_tag="KB942_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6852566.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5917..6816
                     /locus_tag="KB942_002149"
     CDS             5917..6816
                     /locus_tag="KB942_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBB6852567.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            7333..8064
                     /locus_tag="KB942_002150"
     CDS             7333..8064
                     /locus_tag="KB942_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6852568.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            8209..10236
                     /locus_tag="KB942_002151"
     CDS             8209..10236
                     /locus_tag="KB942_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6852569.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            10344..>11387
                     /locus_tag="KB942_002152"
     CDS             10344..>11387
                     /locus_tag="KB942_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6852570.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFI"
BASE COUNT         3671 a         1915 c         2315 g         3486 t
ORIGIN      
        1 aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt tttctttttt
       61 ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc gatatcaatc
      121 acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc aggatttgca
      181 gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag ctggatatat
      241 tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc tggatgtttt
      301 ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc aacagtcata
      361 ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc agggttcact
      421 aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg tgccataaaa
      481 aataccctcc ttcttctctt tgtctttctt acgattatag catattccca ataataccta
      541 aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag aatgaaaatc
      601 attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat tattagttat
      661 tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag aactgctcca
      721 gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa cgatttgttt
      781 gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt tgacccaaaa
      841 taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag gtattttagt
      901 ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag cattttgctt
      961 tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc agaaaaatca
     1021 attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag ttgctacttt
     1081 ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag ggaattttgc
     1141 gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt tgctcttggt
     1201 ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg cagtaaatct
     1261 gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat tggctagtgc
     1321 agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc cgaatcttgt
     1381 ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt tagcaggtag
     1441 tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt atgaaatacc
     1501 agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc tgataagggg
     1561 acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt cttgttttat
     1621 ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt gcatgtgcat
     1681 tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc agtgccacaa
     1741 tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg ggacttgagt
     1801 cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg acgatgcttc
     1861 agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg ctgagtctat
     1921 tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta ctgatgtcag
     1981 ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc atcatggatc
     2041 cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat atccagagtc
     2101 agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta tttcgatatc
     2161 gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc gggatagaca
     2221 cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg acatcaacgg
     2281 ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt tatcgtgtct
     2341 tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct atgctgtttc
     2401 tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca gtgagtgtcg
     2461 tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa aggaagagta
     2521 tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag tggttaaaga
     2581 tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa atggtgctgg
     2641 aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag gtgaaatcta
     2701 tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa aattatcgat
     2761 cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag tagaatttgg
     2821 acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg ttcaggaatc
     2881 actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc tttcaggcgg
     2941 tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt atattttact
     3001 ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc agattttaaa
     3061 aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata tcaactttgc
     3121 tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta cgcaaggaac
     3181 gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg acatacggat
     3241 ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt aggagagaag
     3301 agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta gcagcatgtg
     3361 gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa actgaaacta
     3421 cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat ccagaaaaag
     3481 tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt ggagataagg
     3541 tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa aaagtagaat
     3601 cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag ccagatctaa
     3661 tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt gctccgacca
     3721 ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat gtcactgctt
     3781 taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa ttatctagtg
     3841 caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta gtcacacttg
     3901 tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc atccatgatt
     3961 tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt caaagtgttt
     4021 cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac agaacaaaag
     4081 ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta gcacaaacga
     4141 atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat ttaagcgggg
     4201 gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt aaatagcata
     4261 aataacagag gattaaactc tcatactatg taacaataat gtttcttaca ttcttaaata
     4321 gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta ttgatgattt
     4381 tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat tatctacatc
     4441 atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt ttttcagcat
     4501 caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat aaaacttaaa
     4561 tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc tgctgcttct
     4621 cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat
     4681 tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat ctgctgcttc
     4741 tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
     4801 ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc
     4861 ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag
     4921 cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg
     4981 ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa
     5041 tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt
     5101 atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc
     5161 ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt
     5221 tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct
     5281 ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag
     5341 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
     5401 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
     5461 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
     5521 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
     5581 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
     5641 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
     5701 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
     5761 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
     5821 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
     5881 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
     5941 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
     6001 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
     6061 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
     6121 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
     6181 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
     6241 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
     6301 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
     6361 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
     6421 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
     6481 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
     6541 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
     6601 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
     6661 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
     6721 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
     6781 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
     6841 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
     6901 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg
     6961 cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata
     7021 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca
     7081 ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag ctgtatttct
     7141 agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt
     7201 tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc
     7261 gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa
     7321 agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc
     7381 atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca
     7441 gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag
     7501 aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg
     7561 gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg
     7621 gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat
     7681 gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca
     7741 gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa
     7801 gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta
     7861 acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg
     7921 gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt
     7981 tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca
     8041 ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt
     8101 catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg
     8161 atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg
     8221 aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat catagggtac
     8281 tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact
     8341 tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt
     8401 atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga
     8461 ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc
     8521 gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta
     8581 ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt
     8641 agtttactct cggattttac atttaaatct tatttatact taggaaatca acgagataga
     8701 gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt
     8761 caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct
     8821 tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat
     8881 cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg
     8941 cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca
     9001 tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt
     9061 cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga tctcattagg
     9121 cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa
     9181 gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa
     9241 gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc
     9301 agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc
     9361 gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat
     9421 gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt
     9481 gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa
     9541 gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa
     9601 ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa
     9661 gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat
     9721 aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa
     9781 ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat
     9841 ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta
     9901 agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt
     9961 tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa
    10021 gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt
    10081 ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat
    10141 cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa
    10201 ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa
    10261 ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat
    10321 tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac
    10381 aattatttat aacactccta gttattttag gggcactttt taacaatata tcagtgtcag
    10441 ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa
    10501 aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga
    10561 gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt
    10621 caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg
    10681 atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa
    10741 ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga
    10801 tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat
    10861 cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag
    10921 ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca
    10981 tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa
    11041 aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga
    11101 ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag
    11161 gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga
    11221 gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta
    11281 caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca
    11341 ataattggat ttatatctta gtagcttcac ttttcttatt gtttatt
//
LOCUS       DADVXN010000092        10903 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.095, whole genome shotgun sequence.
ACCESSION   DADVXN010000092 DADVXN010000000
VERSION     DADVXN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.095"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            12..84
                     /locus_tag="KB942_002153"
     tRNA            12..84
                     /locus_tag="KB942_002153"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="KB942_002154"
     tRNA            88..160
                     /locus_tag="KB942_002154"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="KB942_002155"
     tRNA            168..249
                     /locus_tag="KB942_002155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="KB942_002156"
     tRNA            267..339
                     /locus_tag="KB942_002156"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="KB942_002157"
     tRNA            351..422
                     /locus_tag="KB942_002157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="KB942_002158"
     tRNA            439..524
                     /locus_tag="KB942_002158"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="KB942_002159"
     tRNA            535..608
                     /locus_tag="KB942_002159"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="KB942_002160"
     tRNA            618..691
                     /locus_tag="KB942_002160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="KB942_002161"
     tRNA            711..783
                     /locus_tag="KB942_002161"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="KB942_002162"
     tRNA            811..884
                     /locus_tag="KB942_002162"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="KB942_002163"
     tRNA            898..971
                     /locus_tag="KB942_002163"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="KB942_002164"
     tRNA            995..1084
                     /locus_tag="KB942_002164"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="KB942_002165"
     tRNA            1097..1170
                     /locus_tag="KB942_002165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="KB942_002166"
     tRNA            1189..1264
                     /locus_tag="KB942_002166"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="KB942_002167"
     tRNA            1266..1336
                     /locus_tag="KB942_002167"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="KB942_002168"
     tRNA            1350..1423
                     /locus_tag="KB942_002168"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="KB942_002169"
     tRNA            1448..1535
                     /locus_tag="KB942_002169"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="KB942_002170"
     tRNA            1557..1628
                     /locus_tag="KB942_002170"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="KB942_002171"
     tRNA            1651..1740
                     /locus_tag="KB942_002171"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="KB942_002172"
     tRNA            1753..1826
                     /locus_tag="KB942_002172"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="KB942_002173"
     tRNA            1845..1917
                     /locus_tag="KB942_002173"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="KB942_002174"
     tRNA            1921..1993
                     /locus_tag="KB942_002174"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="KB942_002175"
     tRNA            1998..2068
                     /locus_tag="KB942_002175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="KB942_002176"
     tRNA            2081..2154
                     /locus_tag="KB942_002176"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="KB942_002177"
     tRNA            2166..2237
                     /locus_tag="KB942_002177"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="KB942_002178"
     CDS             2503..3696
                     /locus_tag="KB942_002178"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBB6852571.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="KB942_002179"
     CDS             3826..5298
                     /locus_tag="KB942_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852572.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="KB942_002180"
     CDS             5381..6169
                     /locus_tag="KB942_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBB6852573.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="KB942_002181"
     CDS             complement(6236..6904)
                     /locus_tag="KB942_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBB6852574.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="KB942_002182"
     CDS             7019..8731
                     /locus_tag="KB942_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852575.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="KB942_002183"
     CDS             8734..10506
                     /locus_tag="KB942_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852576.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10903)
                     /locus_tag="KB942_002184"
     CDS             complement(10588..>10903)
                     /locus_tag="KB942_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6852577.1"
                     /translation="EENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKK
                     VSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKI
                     EI"
BASE COUNT         3127 a         1964 c         2506 g         3306 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tca
//
LOCUS       DADVXN010000093        10855 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.096, whole genome shotgun sequence.
ACCESSION   DADVXN010000093 DADVXN010000000
VERSION     DADVXN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.096"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            171..986
                     /gene="thiD"
                     /locus_tag="KB942_002185"
     CDS             171..986
                     /gene="thiD"
                     /locus_tag="KB942_002185"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBB6852578.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1315..2766)
                     /gene="cls"
                     /locus_tag="KB942_002186"
     CDS             complement(1315..2766)
                     /gene="cls"
                     /locus_tag="KB942_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6852579.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3107..5773
                     /locus_tag="KB942_002187"
     CDS             3107..5773
                     /locus_tag="KB942_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6852580.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6137..6889
                     /locus_tag="KB942_002188"
     CDS             6137..6889
                     /locus_tag="KB942_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6852581.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6911..8065)
                     /locus_tag="KB942_002189"
     CDS             complement(6911..8065)
                     /locus_tag="KB942_002189"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBB6852582.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8250..10691
                     /locus_tag="KB942_002190"
     CDS             8250..10691
                     /locus_tag="KB942_002190"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBB6852583.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3530 a         1933 c         2328 g         3064 t
ORIGIN      
        1 acaaataaac gaccactatt ttctgattta ttgcaaatgt tttttcaaat aaatttgatt
       61 accaagaaaa ggtagtggta tatttactga gggatacttt gaaaaacata tgatagaaac
      121 agtaacagta ttatgaaaaa tctttttata aaaatggagg aatcgatttg atggaagatc
      181 agtatgtaca agcaatgacg atcgcaggat cagatagcga tggaagcgca gggatgcaag
      241 cagatatgca tacattcttt acaagaaatg tttacggtgt ttctgtcatg accgcatgtg
      301 ttgcaggtaa ttcttacgga atcggagcaa gtgtgacatt gccaacagat tttatcgaca
      361 aagaatttga actgattgcg aaggactttc aaatacgtgc agctaaaaca ggtatgctag
      421 cagattcaaa gttgattgag acagttgtaa aaaattacca aaaatatgat tttggaccat
      481 tagtcgtgga tcccgtaatc gtaacgaaac atggaaatct tttactagaa gaatctgctt
      541 tgcaaagctt gaaagaaaag cttgtccctt tggccgaagt actgacacct aatttttatg
      601 aagcagaaaa gctgactgga cggacattag cagctgaaga agattttgtg caagcagcaa
      661 aagaactgca agatatgggt gcaaaaaacg ttatgatcaa aggacatcat gctaaaaatg
      721 cgcagcaaga aacagtcaat gactacgtat tgttcgaatc aggaaaatca ttttggttaa
      781 atgcaccatt ttatcctacc gaccggatca acggaactgg cgattcgcta tctgcatgta
      841 ttgcagctga gttagcgaaa ggaaaaacag ttgaacaagc gatccgattg gcaaaagact
      901 atgtaaaccg tgcaattcgt gaagaaataa atgtagggca caaatatggc cccatcaatc
      961 attgggcagc tggtgaatta gagtaaaaga aagaagttgt gaaaaagttg ccctgcatca
     1021 agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt tggacttatt
     1081 accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct gccgtccagc
     1141 tccgagtcac aaggaacaag ttcaaaaaac agcttctcat gtttgttgaa tattgtgact
     1201 tgtgaccgag gagttggcag gagaacaccg tttgttcgga agaagggact gtgacaagat
     1261 ttttgtcaca gtccctttaa tcttagtata tttttctcta cttgaataaa aaaattatag
     1321 gattggagaa aataatcgag aaatttgttg cttgaataat agccagttag acatttcctt
     1381 gactgtttca gttgtcatgg atgaacaatg tgtctcatct tctttgaact gagtagcaag
     1441 ttcttctaaa aactcaggat cgtaaatgac agcacttgtt tcaaaattca atcggtagct
     1501 acgaatatct tgatttgctg aacctaccat gcagatttca tcatccatta tcaatgtttt
     1561 tgcatggagg aagccgttct catagacaag gatttgtaca ttttccttga tcagctgccg
     1621 agcataatat tgtgttgctc gataaataaa aggatgatcc ggtttgttgg gaatcatgat
     1681 tttcacatct acaccagagg cagttgcgat ttttaaagca gcaatgacac tttcatcagg
     1741 aacaaggtaa ggtgtctgta tccaaacacg ttttttagca gaagtaatca atttgataaa
     1801 tgaaagcttg atttgttccc tttcgttatt cggtccgctt gaaacaagct ggatggatgt
     1861 atcagcaagc ttagattcat ctcgatctgc ttttttaaag aaataattca aatgatagcc
     1921 gacctttttt tcttcaggta ccgagacgtt ccagtctgtt aagaagcgaa gctggagcaa
     1981 agaagaagcg gcaccgaaaa tccgtatatg cgtatcgcgc caatagccga atttttttgt
     2041 tgtatttaca tattgattgg caatattgaa accaccggta taactaatct ttccatcgat
     2101 cacaacgatt tttcggtgat catggtaatt caatcggaaa cgaagtaatg ccctttgtga
     2161 ggtaacaaat gtatggacat gtccaccatt tttgattaga cgattgaaat cttttgcttt
     2221 tgtgccatga gagccaaatg catcatataa tattcgaact tccacgcctt ctgcggcttt
     2281 ttcttctaaa acatgtaaga cttgctggcc gatattatct gtcacaaatg cgtaatattc
     2341 gatatggatc gaatgtttgg ctttttttag atcttgaagc agtgaatcca atttctcttg
     2401 tccgtctgtg agaagagtga cagaattcat tcttgtcagc ggcatacgat ttaatgaaga
     2461 gaagaagtca acaaattgct gtttgtcttt atcgcccatg tcaggatcgt aatgttcaat
     2521 ggtatctcct ctaaaagact gaaagttttc taattctttc aagtcacttt gtcggagata
     2581 gaattttttc ttatccgtta atccacgtcc gaaaaataaa tataatacaa agcccacccc
     2641 aggaagggca aacaatacta agagccatgc ccaaatggct gctacatctc ggggtttgat
     2701 caaaatagcc accaaagcaa taagtgcatt taatagataa agggcggtta taatactaga
     2761 taccacaaaa atgttcctcc ttacatacaa taacattagt atagcatgat agaagaaaga
     2821 agcctaccaa ggtgaatata cttttcactc ctttcatgaa aatgtaggta aatcgggcta
     2881 aagtagttgc tttttgtagg ttaataaggt aggatattag tacagttgaa tatctcctta
     2941 ggggagtaac cggcaacgtt tgttgcgata atatcaacag taagttaacc aaatggtttt
     3001 ctggtattgt atgaaatggt gagacctaag acaaatgcgt atttgtctta ggtctctttt
     3061 ttttcagaca ggactagggc caagatcaaa aaatggggga tgaaatttgg aaaacaagga
     3121 acaagcacaa caagcggtct ataaagaaac gatcgactcg ttgatgcagc aaacgcagag
     3181 ctcaaccgat ggattgagca ccacgcaagc gaaggaaaaa ttagaaagag atggcttgaa
     3241 ccagctaaaa gaaaagccga agaagccaac ttggaaattg ttcttagaaa ctttcaaaga
     3301 tgcaatggtt atcgtattgc tgattgtcgc actgattcag atggtcatgg gtgactacat
     3361 cgaattctta gttatttttg cagtattgat gttgaattct ataatcagtg ttgtccaaac
     3421 aaaaaaagca gaaggttctt tagaagcgtt gaaaaacctt tcagcgcccg atgctcgagt
     3481 gattcgtgat ggtaaggaaa aaacgattcc ggcaaacgaa cttgtggtag gagatatcgt
     3541 gttgttagac gcaggcgatt atgtacctgc tgatggacgt ttactagatg cgggatcatt
     3601 aaaagtagat gaaggtatgc tgacaggaga gtcgacttct gcagaaaaag aagtaacgga
     3661 tatcacaagt acggtaccaa tcggagatcg tacaaatatg gtattcagtg gaacgatcgt
     3721 aacttacgga cgcggtaaat tcttagtgac ttcaactggg aataatacgg agatcggaca
     3781 agttgctggc cttttggaat caacaactga gcaagtcact ccgttacaac gaggtctgga
     3841 taaattcagt aaaaaactaa gtttggctat tttggcattg tctttattga ttttaggtat
     3901 ccaactgttt cgtatttatc ttggagaagg tacaggagat atgactgcag atatcgtcag
     3961 cgcagtgatg tttgccgtag ccgtagccgt agctgctatt ccagaagcat tacagtctat
     4021 cgtaacaatc gtgttatctt tgggaacgaa taaaatggca aaacgtcatg ccattattcg
     4081 gaagcttcca gcagttgaaa cacttggttc gacaagtatc atctgtacag ataagactgg
     4141 tacattgaca caaaacaaaa tgacagttgt tgactacttt ttaggtaatg gcgatactgg
     4201 ggatttcaca aatgaccctt caaaatggac agctgacgaa cgtcgcttga tcgaaatcag
     4261 tgttttagcg aatgatgcat cgatcagtga agacggtact aaattgggcg accctacgga
     4321 agtagcgttt atcgacttta gtgaaaaatt aaaccagcct tatcaggaaa tccgaaacaa
     4381 ctacccaaga caagcagaac ttccttttga ctcagatcga aaattaatgt ctactggaca
     4441 cacaattgat aacaaaaatt acttgtttgt taaaggcgga ccagatatcg tcttccaacg
     4501 cagtaaaaaa gtattgctaa atggtgaagt cgttccgatg acggatgaat tgatgaaaaa
     4561 attccaagag caaaatgaag ctttttcaac acgcgcatta cgtgtactag cttttgctta
     4621 ccgtccactt gataattttg atttatcatt tgaagatgaa gacgatttga tccttgttgg
     4681 tttgcttgct atgatcgatc ctccacgtgc tgaagtgact caggcggtaa cagaagcaag
     4741 aagtgcagga atcaaaacaa tcatgattac tggtgaccac aaaacaacag ctgttgctat
     4801 cgcaaaacaa atcgggattt ttgaagaagg aaacctggct ttgaccggga cggaactaga
     4861 tgaattgaca gaagatcaac taaacgaagt tttagaaaaa gtcactgttt atgcacgtgt
     4921 ttcacctgag aataaaattc gtatcgttcg tgcttggcaa aataaaggtc atatctcagc
     4981 tatgacaggt gatggtgtaa atgacgcacc agcattgaaa caagcagaca tcggaattgc
     5041 tatgggaacg ggtacagatg tggcaaaaga tgcagcagcg atggtattga cggatgataa
     5101 ctttgcttct attgtcagcg cagtagaagt aggacgtaat gtgtttgata atatcaagaa
     5161 agctatttct tatctatttg caggaaattt aggtgcgatc attgcgatca tctttgcctt
     5221 agttgctgac tggtccaatc catttacagc attacaactg ttgttcatca atttagtcaa
     5281 tgattcactg ccagcaattg cattaggtac agaaaaagct gaacctgcta cgatgaaccg
     5341 cccgccaaga gatccaaatt caggaatctt tacggacaaa acattgattt ctgtaacgta
     5401 tcgtggtgtg ctgattggga ttgctgttat ctttgctcaa tggcttggca tgcagcattc
     5461 acctgaattc ggtgtagcca tgtcattttc aacattgatt tggtgtcgga cattgcaaac
     5521 attcccagca cgttcaaaca cacaaacagc tatccaagca gggttcttct ctaacagaag
     5581 tgtcttacta gctgtagttg tatgtagcgc gctttactgc ttaacaatga tcccaggact
     5641 gaaagaagtc ttctctatcc catcagcatt tggattgaca gaagtcttta catgtatggg
     5701 aatcgcactt gtgacaattg ttttgatgga aatcactaaa ttgatcattg tacgagtaca
     5761 aggaaataaa taaaacgaat caacgaggtt gtgagcctgc cgtttaattc tgagtcacaa
     5821 ggtgcaattt caacaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgaaga
     5881 gttggcagga gaacaccgtt taaggaagaa gttgtaaaat cagcattttg agctaatact
     5941 gagggtcatc tgattggaca ctgtttattc ggaaagaggg actttgacac gactttggtc
     6001 acagtccctt tttttattta tgatagacgg aaagccaatc attttattat tcatcaaaag
     6061 agatagaata gataaaagag gagaatgatt tattccgaaa agtgaaaaca tataacaaaa
     6121 aattagagga aatcaaatga atgaaaaaga atgggatgaa tttgctgaag aatattatga
     6181 aatccagcaa gaatcgcaag taactattgc tgaagatgta aaaaattatc tgaaaacgga
     6241 gaaagtcctg cctgctgaaa cagcagcaga cgttgctgga ggatctggca gatttctttc
     6301 cttagcaaaa gaaattaatc gctatgattt gatcgatttt tcttcaggaa tgctagaaaa
     6361 tgcaaaaaaa gaagcagaaa aaaatgagtt gccaaatgtt tcctttatca aacagtcgtt
     6421 agaagaattt atccaatcaa atgagacata tgaaatggtt tttacagcag caaatccagc
     6481 attggtacat cttattcaat tagaaaaatt gatgaaaaag tcaagcaaat ggtgcgtgat
     6541 aatcagagta gtggaatcaa tcgatgatgt tttttatcct atcgaaaagc aactaggaat
     6601 gatagaagaa gatcctaata catcgccagt tgtaatggat caatttgaaa catacttaat
     6661 tgatgaagga ttcaaagtga agaaaaaaga ttttacttat tttacacagg aatcaattac
     6721 tgaagaattg atgaagctat actatgacgc gtacgcaaaa gatcctgttg tgaccaggta
     6781 cattcgcaaa ctattcagtg aaaaacgaga aatccttagt acgactaagc tgacctatag
     6841 actgctcctt tggtccgcag ataaaaaaag aacctttcac gactcgtgaa gggttctttt
     6901 tatgatttta ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag
     6961 gtacatctga agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg
     7021 tttcatattc gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg
     7081 cgaagagata ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac
     7141 cagaaccgta gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca
     7201 gtagagaagt tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt
     7261 aacggatgct ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt
     7321 ctaatacgct ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg
     7381 cctgataatc ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga
     7441 atgattcgat atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat
     7501 ctggacgcca gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata
     7561 aaatacgcgg gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac
     7621 caccgcttgc caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg
     7681 gatgattttt gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct
     7741 cttttacttc aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg
     7801 cactcgcttt tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct
     7861 cacggtcttt ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt
     7921 catttgcacg attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat
     7981 catccccacg gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg
     8041 aaagacgatc aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact
     8101 ttactaaggt atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt
     8161 taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga
     8221 atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa
     8281 gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca
     8341 cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag
     8401 taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt
     8461 tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg
     8521 gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga
     8581 tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa
     8641 caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt
     8701 caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag
     8761 aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg
     8821 gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg
     8881 atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct
     8941 cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg
     9001 tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca
     9061 aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca
     9121 ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca
     9181 atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa
     9241 aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac
     9301 gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt
     9361 cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa
     9421 cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc
     9481 aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac
     9541 tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa
     9601 tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag
     9661 aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg
     9721 cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc
     9781 aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa
     9841 gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca
     9901 tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg
     9961 gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc
    10021 ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg
    10081 cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg
    10141 ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc
    10201 acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac
    10261 gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa
    10321 cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg
    10381 ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag
    10441 atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc
    10501 tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag
    10561 ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa
    10621 aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc
    10681 gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt
    10741 tactgcagat cttgcttttt tggctcttcg tcaaattgtg ttggcgaaga caaatttgaa
    10801 taaaacttag tccgatatgc ggcgcgttct gtgcgccgca tattttttta gtttt
//
LOCUS       DADVXN010000094        10684 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.097, whole genome shotgun sequence.
ACCESSION   DADVXN010000094 DADVXN010000000
VERSION     DADVXN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.097"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(150..557)
                     /locus_tag="KB942_002191"
     CDS             complement(150..557)
                     /locus_tag="KB942_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852584.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            718..942
                     /locus_tag="KB942_002192"
     CDS             718..942
                     /locus_tag="KB942_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852585.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            952..1146
                     /locus_tag="KB942_002193"
     CDS             952..1146
                     /locus_tag="KB942_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852586.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            1149..2171
                     /locus_tag="KB942_002194"
     CDS             1149..2171
                     /locus_tag="KB942_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6852587.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            2134..2949
                     /locus_tag="KB942_002195"
     CDS             2134..2949
                     /locus_tag="KB942_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBB6852588.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            2967..3749
                     /locus_tag="KB942_002196"
     CDS             2967..3749
                     /locus_tag="KB942_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852589.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            3749..4597
                     /locus_tag="KB942_002197"
     CDS             3749..4597
                     /locus_tag="KB942_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6852590.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            4594..4836
                     /locus_tag="KB942_002198"
     CDS             4594..4836
                     /locus_tag="KB942_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852591.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            4979..5347
                     /locus_tag="KB942_002199"
     CDS             4979..5347
                     /locus_tag="KB942_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBB6852592.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            5362..5523
                     /locus_tag="KB942_002200"
     CDS             5362..5523
                     /locus_tag="KB942_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6852593.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5520..5846
                     /locus_tag="KB942_002201"
     CDS             5520..5846
                     /locus_tag="KB942_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852594.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            5843..6658
                     /locus_tag="KB942_002202"
     CDS             5843..6658
                     /locus_tag="KB942_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBB6852595.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            6797..7108
                     /locus_tag="KB942_002203"
     CDS             6797..7108
                     /locus_tag="KB942_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6852596.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            7132..7350
                     /locus_tag="KB942_002204"
     CDS             7132..7350
                     /locus_tag="KB942_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852597.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7347..7763
                     /locus_tag="KB942_002205"
     CDS             7347..7763
                     /locus_tag="KB942_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBB6852598.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            7760..8098
                     /locus_tag="KB942_002206"
     CDS             7760..8098
                     /locus_tag="KB942_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852599.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            8095..8325
                     /locus_tag="KB942_002207"
     CDS             8095..8325
                     /locus_tag="KB942_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852600.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            8332..8535
                     /locus_tag="KB942_002208"
     CDS             8332..8535
                     /locus_tag="KB942_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6852601.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            8532..8828
                     /locus_tag="KB942_002209"
     CDS             8532..8828
                     /locus_tag="KB942_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852602.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            8905..9318
                     /locus_tag="KB942_002210"
     CDS             8905..9318
                     /locus_tag="KB942_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6852603.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9331..9717
                     /locus_tag="KB942_002211"
     CDS             9331..9717
                     /locus_tag="KB942_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852604.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            10268..10495
                     /locus_tag="KB942_002212"
     CDS             10268..10495
                     /locus_tag="KB942_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852605.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            10492..>10684
                     /locus_tag="KB942_002213"
     CDS             10492..>10684
                     /locus_tag="KB942_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852606.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         3855 a         1651 c         2424 g         2754 t
ORIGIN      
        1 tacagagcga aaaagcccac cgagggggct gactaaagtg tggttctgtt tattaggtgt
       61 ttatctcgtg gccgttttag taaataacta catgagaaaa cgtggcgaat actggtatac
      121 atcctatgcg gttctagtat gtctaatgct tacaattttt ctaatgattt attcaaaata
      181 ggtaatactt tgtcaatcaa ataagtttca agagattttt gttctgcatc agtgttgccg
      241 taaccaaaac ggcttaagaa aattttcata acctctatat cttcattttg aatatatggc
      301 aggactgcat aacctgatgc ttttacagca gatatatttt tatcagtgtt actaccaata
      361 caaataccca cattgttgag aaatgttgag aaagttgttt gtattgtttg ggtttttgaa
      421 tcgtgttttt gtaattcaat ttctttatcc ttcatcgatt caacatgctt attatttata
      481 accgctgtaa tcgaagggga tataagagct actaaagcta ggataatcga aattgtgatc
      541 gtgttatcga aactcatttt ttcaccatcc agtttttaaa ccattatatc aaaaaggaga
      601 gaagaaataa tgcaagaatt agtaattttg aaaaataaaa aaagacacga acgccagcaa
      661 gcatgatcgt gtccaaaaga aaaatctttt aaaccattat aacacaggag gaaataaatg
      721 atctcagtta aagggttagg cgatgaaata ttcgaagcaa tgatgagcaa agcacaacag
      781 gacatcaaag aaaaaatcat ttctgctgca gcgcaaggac aaactagctg cactgttcga
      841 tcgagaggaa cgacaccaag ttttcttttg gcgctagaag atgaaggcat tttgaatatc
      901 aaacgaagag atggcagcgt cagattgttt tggcaatttt aggaggccgt gatgaaagat
      961 tttgattctt taggcgccag acagcaacca ccaaatgaag ctagtcctgt tggagttgat
     1021 tggcaagaaa atccgttata tccaggtgat acttgctatc taacagaaga aggctatgta
     1081 ccagtagacg caattctaga gtacgtccag caacattatc caaagattga attaggagga
     1141 atttaggaat ggcaaatgat ttaacacaaa caacacaacg ttcattagat gagcaggtaa
     1201 tcagtaatct tggacggtta caagaacaag gtttagaaat gccaccagga tatagtccac
     1261 aaaatgcgtt aaaaagtgct tttttcgaac tcactaacaa tacaggagga aacttgttgc
     1321 agatggctgc aaacaatcaa gaaatgaaaa cctccatttc taatgcactt ttagacatgg
     1381 ttattcaagg gttatctcca gcaaaaaaac aatgctattt cattaaatac ggaaacaagg
     1441 ttcagctaat gcgttcatat tttggaacaa tggcggtcct tgatcgtgta actggagggg
     1501 cagatattac gcctgtagtc gttcgacaag gtgatgagtt tgaagttgca atggatggac
     1561 caaacatggt tgtcaaaaag catgaaacaa aattcgagaa tttagacaat gaaatcattg
     1621 cagcctatgt agtcatcaag ttaacaaatg gtaaagagac cacaacagtc atgacgaaaa
     1681 agcaaatcga tcaaagttgg gctaagtcaa aaatgaaaga ttctggaccg caaaaggaat
     1741 ttccagaaga aatggctaaa cggacagtaa tcaatcgggc agctaaaaca ttgatcaaca
     1801 caagcaacga caacgattta ttagttcaag ctgcaaaaga tacgttagaa aatgaatttg
     1861 acaatgatcg gaaagatata acgccacaaa cagaaaaagt ggctactctc gaacaaaaat
     1921 tcttttcaaa caaaaagatt actgaaccaa tacaaaaaga accagatccg attgtgattc
     1981 cagatgatat ccaaaatgaa gtaactcgag ttgctgatgt acctgcccat ccagaaatag
     2041 agcaagcaca tttgatcgaa aatgaggata tcaatccaat tcaagaagaa ttgatagata
     2101 ttccagactt tggacgcgag gaaggtgtag aagatgtctc agaatttgaa gacgacgagt
     2161 accctttctg atgagaatta ttactccaat gaagctgact ggcagtacat gtcaacatca
     2221 caatataagt cctttttaaa atgtgaagcc gcagccttag caaagttgaa aggcgattgg
     2281 gcaccgacgt cggatcccaa agccttactc gtaggaaact acgtacattc ttattttgaa
     2341 tcaaaagaag tacatgaagc gttcaaagaa gaaaataaat ccaaaatgtt ttctagtagg
     2401 aagccgtatg gattgttgaa agatttccaa attgctgaac aaatgattga acgacttaag
     2461 caagaagaag cattcatgaa catttatcaa ggtgagaaag aaacgatcgt gactggggaa
     2521 ctatttggaa cgacatggaa aggcaagatt gattgcttga atgttgagga tgagtatttt
     2581 gttgatatca aaacaaccaa agacatgcat gagcggaaat ggaacgaaaa ctacggatca
     2641 agagaaacgt tcattgtcaa ttttggttac gtacttcaaa tggcaatcta tcaagagctt
     2701 cttttacagc aatacggaaa gatgttcatg ccaatcatcg ctgcggtatc gaagcaaacg
     2761 cctagtgagg cgaagttgat aacgattgat caagataaga tgagctacga gctaatcatg
     2821 ctaaaagaaa atattgaaag aattgtcaaa gtgaaaaatg gcgaagaaag gccgatacct
     2881 tgcggtacgt gcgagtattg tcgaggtaac ctttcaatta ctggattcac tagcatggac
     2941 gatctttaaa aggaggtgct ttgcgaatga atctaggata catcaagtta taccgtaaag
     3001 tgactagttc attcgtttgg accaattccg atatgttcaa gctatggata ttgtgtttaa
     3061 tgaaggctag tcatgaagat agaaaatttt tatttaacgg tcaagaagta cgtttgacaa
     3121 gcggacaatt cgtcacagga gcccatgcaa tagcaaaaga gtacaacgaa ggagtcccga
     3181 gtgacaaagc aattgcatgg cgaacgctat ggagatggct taagaaattt gaaaatgaag
     3241 aattattgac aatccaatcg aacgctagat atagcgttat aacaataata aattggtctg
     3301 actatcaatc gagtgacaag ccagtgacaa gcgacggaca atcgagtgac aagccagtga
     3361 caacaaacaa gaatgataag aatgataaga atgataagaa taataatcct cgcaactctc
     3421 gaaaaacacg agagtatgca gatgacgatc caaataaaaa attggccatt cttttattaa
     3481 aactcattcg aaagaatcgt agtctcaaag aacccgattt gaacaaatgg gccaatacga
     3541 ttcgtttgac gattgaagct gacaaacgaa ctggaaaaga agttcaagac atgattgttt
     3601 gggctactaa ccatgatttt tggtccacag tcatttcatc gccaactagc ttaagaaaaa
     3661 atttcgatac aatggtgggt caaaaaaata aaagaaagca acaaaatatt tctaatgatg
     3721 agttaccaga aacaggtgag gattggtaat gaataaaaaa ctaagtgcaa tggctgcacc
     3781 ttacggcgga ttaaaaacag cagatcataa ttgcccgaaa tgtggtgatc cattgtatat
     3841 ctggaaaaca aaaaataaag atggtactga tcgatgcggt cctacatgta tcaataagat
     3901 ttgtggttat cgagaaatgg taactaaaaa tcaaaaagaa gctatccaaa aagcgaatga
     3961 agcaatgaaa aaggacgcta tcaatcgaat gcttaacagt tcaatgatta cagatgatgc
     4021 catatggacc ttcgattttg atggatacaa agtagttgat caggaaacag cacaaataaa
     4081 agcgatggct caagaatggg ctaaaaaaat tgtaaatggt agcacgattc atgcagtgat
     4141 aaccggtaga acaggagccg gcaaaaccca tctaggcgct gcagtgatta aagaagtcat
     4201 gagggcatct aattataaaa ttgcttgttc atttatcagt tatcgagaac ttttagaaca
     4261 gttgaaattt gcaatgaatg atccagaagc aagaaaagcc gtaacaggtt cgctgatggc
     4321 tgagataaag aaatcagatt tcgtggtaat cgacgatttg ggtgcagaac taggaagaat
     4381 ggaagaaaac aaccaggcaa cgccttatga tgttgatgtt ctcacatcgc tcacagaagc
     4441 tcgtctaaac aaagctacga tattcacaac caatttatca tcgaagcaat taaaacacgc
     4501 gtacggtgag cgagtgttct ctcgtgtaat gaatggaacg aaagggaaca tagctgtatt
     4561 caaaacaacg acagacaaaa ggaggaatcc agtttgacct ttgtagtgaa aaaaatgtgc
     4621 tacttagata gccgagggcg aggagaagct agtgtcgagt ttgctaaaca ccacacgact
     4681 aaagaagagg ctgaccttgt tgcaagtgtt tgtggcggcg aagtagtcga agttattaaa
     4741 cccaaaagac gattttcaaa acctaagaca aagccagtga aaaaagagtg ttgttgtccg
     4801 aaaagtaacc aagcatggat gagaggtgca aaatgacttg tttaaaatgc aacgatgaaa
     4861 cagttatttg gtataagaca ttgcttggat ggtcaacttg tgaaccttgt ccaatatgca
     4921 atgaaaatgg acgacgttcg aaagaacgac tcgaaagact aaagaaggag tatagcaaat
     4981 ggcaacaaga agcaaatacg gaaacaaaaa gcacgaagta gacggcatca cttttgattc
     5041 tatcacggaa gcagagtatt acgatctagc cttgtggcaa gcacaggcga atggttggaa
     5101 agtgaagctc caggaaaggt ttgagctgat gccgaagttt gaactagagg gcaagaagta
     5161 tcgcaagatc gagtatattc ccgacttcac attttacaaa agcggcaagc tggtcaaagt
     5221 cgtagatgtt aaggggatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca
     5281 tcaatatcaa gtgccgttga tattagccaa aaaatatcgg aatacgttca aggaagagcg
     5341 tttttaacga ggtggtccat catgacaaca gaagaagtga ttcaaatgcg aattcgaagc
     5401 cttcggcgtg agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggct
     5461 agaaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccgtag attggaggaa
     5521 tgagcgtgga taaggaagcg gcttggcgaa aattaatgtt gctgattcaa gatgagaact
     5581 ggcaagaaga tgaagcagtg gttgctgaag ttcagcgtct aggaaagatt gctaacggac
     5641 gtatacgaaa aaagccagac aaaagaaaac agcgcaaagg gaaaatcgtc gttgttttac
     5701 acgaaggcaa aattttgatg caaggaacag ctagtgagct gtctgcagaa actggatata
     5761 cgcgtgggac tattcgaacg tacgcttggc gaaatcacac cgatcgaaaa gggcacgaat
     5821 ataagtattt ggaggaagaa aaatgaacga aaacaaatta atcaaattgg gtgtagcagg
     5881 agcagtaatc gtaggtattg gagttatcgg aggatttaag ttcttcgaaa aaatcgataa
     5941 tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc aaagatgaag cactgacgca
     6001 aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat ccaattcgct tgcaaactat
     6061 ccaatcaaaa aatatttcag tatctacaag cgacggcaaa aagacaacga ttgatatcaa
     6121 atatgactac aaagttgatt caactaaagc agcaaaaatg tacaaggaat ttgggaatat
     6181 cacttcggaa gatatcgaaa gtggatggtt aaaatctaag cttcaaaagg tcgctcgtga
     6241 agtttatgcg aaatatagtc tgcttgatgt cctttcagga gattcctcta aagttgaagc
     6301 tgaggtatta acgaactttg ctaaatcagt tgaatctaaa gggtttgaag tcgaagacgt
     6361 aacacttggt gttccagatg tcgataaaga aacacaaaaa tcaatcgatg cgatcattcg
     6421 agctggtcaa gaaaatgaaa aagcgaagct agacgcagaa actgcaaaaa ctcaagctga
     6481 tagtgaagct tacaagaaaa caaaagctgc agaagcagag gcagaatcta atcgcaaagt
     6541 cgccgaatca gtaacagaca atttgattcg ttatgaagaa gctcaagctc gcaaaaagca
     6601 tggatgggta acagtaaatg gagcagatac tgtagttacg gatgaagcag gcaaataata
     6661 tgggattctt tatggctaaa attctcttgt tcttaggttt agtaggagca gcatatctcg
     6721 tgtatgcgct cctttcccaa actgatgaca aagaagatga caacaacgat gaaatgaaat
     6781 tttaggagga gaaataatgg acgaactaat cacaaaagta gagcagtggg ctaaagacaa
     6841 gggactggat caagcagatc caaaagcaca gtttttgaaa gtagctgagg aattcgggga
     6901 aatagcttcg gcgatggcaa gaagtaatga tgaactattt aaagatagcg taggagacgt
     6961 tatcgtcacg ctgattatcc tttccatgca aaaagggaca aacgtacaag agtgtttaga
     7021 aatggcatac aacgaaatca aaggacgcac agggaaaatg gtagatggtg tattcgtgaa
     7081 gtcgagtgat ttggagggca gcaaatgata aaaaagctca ttcaattcag catggattta
     7141 tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt
     7201 ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt
     7261 catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
     7321 attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg gataaacgaa
     7381 agaacgtaat gagagatgta gccgtcttgc attttactaa aaacggcaaa acaaacttta
     7441 ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca
     7501 tgcaatccac aggaatgaaa gataagaatg gtgtggatat atttgaaggg gatatagtat
     7561 tagtcagcgt gcgaaatggc ttcgattact tagataataa agtttgtatt gtcaaaaatt
     7621 caatagatta ttccggatta gtttgtgcca ctgttgatga agacttagag tatcaaattt
     7681 ttaacacaga gctgtttgaa gaatacacgt atgaagtcat cggaaatata tacgagaata
     7741 gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt ctttaaaaga
     7801 tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga atagaatcat
     7861 tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga cagcagtata
     7921 ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg attattatga
     7981 tcgtttaatc agtcctgata tgtatgggaa ttcagaatca gcaattaagc tgtttggcgc
     8041 aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag cggaatgaaa
     8101 tacgaaatac cactaagtga agcagacgtc cagtcaatta ttaacggtcg ggaggttaat
     8161 aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa agatatggca
     8221 gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa ccacttgaaa
     8281 gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac aatgaacacc
     8341 agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa gacaaagttt
     8401 gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct
     8461 gatgcaagtg atgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg
     8521 gaggaaaaag aatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt
     8581 taatgctaga tatgccgaca aagaatccta aaacacgtaa gaaaccaaaa gtcaaagtcg
     8641 gtgacatggt ccgctgtgaa gcagaggagt tcatttatcc atttcgtgga tatgtagaac
     8701 acgtctataa tcactcagcg atcattcgca ttgaaaacac gatggaatgt gataaatgga
     8761 cagctaaaag caaagagaat ttagcagtag tgagattggt ggatatggaa ctaatcaatg
     8821 acaaataaaa aagccggatc gctccgacta acataataaa acagacaagt ttattatatc
     8881 acataaaagg agcagtttga attgatgcaa ttgttacgag aggtagattt caaacagaca
     8941 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
     9001 tctttgatag atattaagtc gccgattatt acggatatgc cgaaggcacc gaagcacggc
     9061 aataaggcag aggatgcatt gattcagatg atggatatag aagcggagag agacgcgatt
     9121 ttagcggctt tgatggctct tagtctgatt agtcgtcaga tactctacta cagtttttgt
     9181 gtgccagaca gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt
     9241 atacaacgga tgaaatcgga agctctaata gagtttgcag aagcatataa acacggaaga
     9301 ataattgctt ataaataggg ggaccatata atgtggaaag attatgtatc gttgaaagag
     9361 ttgaaaaaag atcttgtttt caaaagaatt gtagaatggt cagagagtga attaatttta
     9421 gaagatggaa ccaaaatgga agttgtatgt agcgaatcag attgctgcgc atgggccgaa
     9481 ggtgaattca aaaatgtaaa gcttgatgca gtgattacag atataaagat ttttgataaa
     9541 ggtaaccgtc tctataatgg tgacggacat acctcttatg ctgaagtcgt tgtttatcat
     9601 aatcgtaatg agatcgctaa agcagaatgc acagcgaatg atgggaatgg cggctactat
     9661 tatagtgttt gtgctttaaa ggtcaaagac aaactttgta tagtgactga cgcataaaaa
     9721 aatggcggtt ttttggcgga atgatggcgg tttttagcca tttatcagtg atattatggt
     9781 agtgtcgaaa gattagtgat aggtctgaga caaaataata ataaaaggaa catcgtttta
     9841 ttattgtttc acaattaagc ttcgatagac agcaacggaa atattaagaa taaggatgtg
     9901 aattttaact ccttctaaat tgttcttatt atctatcatc cgttgctgtt tattgttaca
     9961 tacagatcgc ttaggcggtc tttttatttt ggccgaaaac ctacattttc gatggccgat
    10021 tattggagga ggaatagcat gttcaaacta tctgaaatca ttaagaagtc cgacgttgag
    10081 aaattagaga tgttaaaaaa gaagttgaag aaatagcagt ctcttcgtga ggctgtttta
    10141 ttttgctcac aaaaatagac cactatcggg taatagtggt caggaattaa atgaaaaaga
    10201 tgttagggtt gttagctaag tataacatca taacgcttac aaaacaatgc aagaaggata
    10261 aattattatg agaacttact ggtatgtgtc attaaacaat aaatatccga agccaatgaa
    10321 aggacagcat aggcgtgtag tcatgtctgt tcaaatgaag gcgaagtatt cgattgtaga
    10381 aatgctaaga gaagctacac caaaagagat tgatcagtat aagttggttc ttattggcat
    10441 tggttggttt aaggatgaac atattcaaac aaacatgaag aggtggttga gatgaagtta
    10501 tattatgttg aaaaagcaaa ttgttgggtg gttgaaggct gtctgtcaaa gtacgctaca
    10561 gatattatat ctactcaatt ttcagagttg ggattgaagg ttatcttttg tggtgagttt
    10621 gtcagtgtga aagaagcggt attgccaatg acagcaaaga gcagacgctc gcgtggttca
    10681 agtt
//
LOCUS       DADVXN010000095        10584 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.098, whole genome shotgun sequence.
ACCESSION   DADVXN010000095 DADVXN010000000
VERSION     DADVXN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.098"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            33..656
                     /locus_tag="KB942_002214"
     CDS             33..656
                     /locus_tag="KB942_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852607.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="KB942_002215"
     CDS             1106..2155
                     /locus_tag="KB942_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852608.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="KB942_002216"
     CDS             2152..3033
                     /locus_tag="KB942_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852609.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="KB942_002217"
     CDS             3036..3806
                     /locus_tag="KB942_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6852610.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="KB942_002218"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="KB942_002218"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBB6852611.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="KB942_002219"
     CDS             5117..6280
                     /locus_tag="KB942_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6852612.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="KB942_002220"
     CDS             6397..6807
                     /locus_tag="KB942_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6852613.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="KB942_002221"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="KB942_002221"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6852614.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="KB942_002222"
     CDS             9380..9664
                     /locus_tag="KB942_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852615.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="KB942_002223"
     CDS             9824..10060
                     /locus_tag="KB942_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6852616.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="KB942_002224"
     CDS             10057..10455
                     /locus_tag="KB942_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6852617.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3696 a         1770 c         2235 g         2883 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataa
//
LOCUS       DADVXN010000096        10266 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.099, whole genome shotgun sequence.
ACCESSION   DADVXN010000096 DADVXN010000000
VERSION     DADVXN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.099"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..465
                     /locus_tag="KB942_002225"
     CDS             <1..465
                     /locus_tag="KB942_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBB6852618.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="KB942_002226"
     CDS             complement(1080..2120)
                     /locus_tag="KB942_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6852619.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="KB942_002227"
     CDS             complement(2469..2795)
                     /locus_tag="KB942_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852620.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3585)
                     /locus_tag="KB942_002228"
     CDS             complement(2782..3585)
                     /locus_tag="KB942_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6852621.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="KB942_002229"
     CDS             complement(3938..4228)
                     /locus_tag="KB942_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6852622.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="KB942_002230"
     CDS             complement(4364..4543)
                     /locus_tag="KB942_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852623.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="KB942_002231"
     CDS             complement(4540..4890)
                     /locus_tag="KB942_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852624.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="KB942_002232"
     CDS             complement(4883..6208)
                     /locus_tag="KB942_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6852625.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="KB942_002233"
     CDS             6658..7272
                     /locus_tag="KB942_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6852626.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="KB942_002234"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="KB942_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="KB942_002235"
     CDS             complement(7883..9202)
                     /locus_tag="KB942_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBB6852627.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9357..9944)
                     /locus_tag="KB942_002236"
                     /pseudo
     CDS             complement(9357..9944)
                     /locus_tag="KB942_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3181 a         1832 c         1599 g         3654 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaa
//
LOCUS       DADVXN010000097        10220 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.100, whole genome shotgun sequence.
ACCESSION   DADVXN010000097 DADVXN010000000
VERSION     DADVXN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.100"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            355..624
                     /gene="rpsN"
                     /locus_tag="KB942_002237"
     CDS             355..624
                     /gene="rpsN"
                     /locus_tag="KB942_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6852628.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            639..818
                     /gene="rpmF"
                     /locus_tag="KB942_002238"
     CDS             639..818
                     /gene="rpmF"
                     /locus_tag="KB942_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6852629.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            850..1227
                     /locus_tag="KB942_002239"
     CDS             850..1227
                     /locus_tag="KB942_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852630.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1248..1397
                     /gene="rpmG"
                     /locus_tag="KB942_002240"
     CDS             1248..1397
                     /gene="rpmG"
                     /locus_tag="KB942_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6852631.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1421..2404
                     /locus_tag="KB942_002241"
     CDS             1421..2404
                     /locus_tag="KB942_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBB6852632.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2434..2556
                     /locus_tag="KB942_002242"
     CDS             2434..2556
                     /locus_tag="KB942_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6852633.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2574..4097
                     /locus_tag="KB942_002243"
     CDS             2574..4097
                     /locus_tag="KB942_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBB6852634.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4121..4360
                     /locus_tag="KB942_002244"
     CDS             4121..4360
                     /locus_tag="KB942_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852635.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4553..5014)
                     /locus_tag="KB942_002245"
     CDS             complement(4553..5014)
                     /locus_tag="KB942_002245"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6852636.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5209..5427)
                     /locus_tag="KB942_002246"
     CDS             complement(5209..5427)
                     /locus_tag="KB942_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852637.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5545..7161
                     /locus_tag="KB942_002247"
     CDS             5545..7161
                     /locus_tag="KB942_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6852638.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7402..8742)
                     /locus_tag="KB942_002248"
                     /pseudo
     CDS             complement(7402..8742)
                     /locus_tag="KB942_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9018..9173)
                     /locus_tag="KB942_002249"
     CDS             complement(9018..9173)
                     /locus_tag="KB942_002249"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBB6852639.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9469..9801)
                     /locus_tag="KB942_002250"
     CDS             complement(9469..9801)
                     /locus_tag="KB942_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6852640.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3407 a         1616 c         2015 g         3182 t
ORIGIN      
        1 ttaaaaaatt ggcgataaac cagttaaaca taaaagattg aataaaaagt tatagtttct
       61 ttcatattgc cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg
      121 actaataaaa gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt
      181 gattatctct atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta
      241 tttcatgtta tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac
      301 gtaacgatta cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca
      361 aaaaagtcga aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat
      421 aaacgtcagg aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat
      481 tcaaatccga atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg
      541 cgaaagttcg gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct
      601 ggagtaaaaa aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa
      661 catctaaggc gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt
      721 catttgacga agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact
      781 ataatggaaa gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag
      841 ggaatcaaaa tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt
      901 gaaacgaccc agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga
      961 attattactg aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat
     1021 tactatgagg accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa
     1081 gagtatgacg aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg
     1141 aaagaaaatt tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa
     1201 attcaagcat atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata
     1261 ttattttgga atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga
     1321 ataaccctga acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt
     1381 ttaaagaagt gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat
     1441 cgtttctggg tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc
     1501 accctacccc agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga
     1561 tcacaaatta ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg
     1621 ttgcagtctt cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga
     1681 acatcaacaa ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc
     1741 aatcattcaa acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt
     1801 attgactgtt attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa
     1861 gcaacttgtc atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc
     1921 actaaaaaaa ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt
     1981 cacacatagt gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc
     2041 cttagttttt gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca
     2101 ccatcaagta catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga
     2161 ttttttactc caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt
     2221 taaaggattg attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga
     2281 acaggttaat tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg
     2341 aaaaatgttg gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa
     2401 ataatgataa gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca
     2461 tatcgacgac ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa
     2521 gaacataaac gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga
     2581 aaatcagtgt aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca
     2641 ctaatgatgc aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct
     2701 atccaatgta tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg
     2761 ttccagcggg atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc
     2821 atgatgctga tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta
     2881 aagaaagttg gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat
     2941 tgccaggtag tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag
     3001 atcctcatac gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc
     3061 agttagttaa attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt
     3121 tgactaaact gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa
     3181 aaagttttgt gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac
     3241 aagttcctat tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat
     3301 taaaagagta tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg
     3361 ataaaattgc aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc
     3421 ttgaaagtct tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag
     3481 acaatctaaa agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa
     3541 aagaagcgaa ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg
     3601 atcgcgaatt gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg
     3661 gaattttgga tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg
     3721 agtacaaaga ttattatact actggctata agacagatat tgatactatc aacattaaag
     3781 acaacaccat tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg
     3841 gatacaaaat tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg
     3901 atgatgcagg tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa
     3961 gtaaggcaga gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg
     4021 aattgcataa ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag
     4081 aaatgatggc tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt
     4141 gtctatggat tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag
     4201 attaaatggg agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt
     4261 tcatagagta ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag
     4321 tagacgttta gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat
     4381 catttttccc ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag
     4441 cgcaacactt tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt
     4501 ctgttattca gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct
     4561 ttcgattttg gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc
     4621 caagggcatg aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga
     4681 aatgattgga gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa
     4741 tactgcttaa aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct
     4801 tctttcctgc taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac
     4861 cagttttgcc agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg
     4921 attttggacg gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat
     4981 tttttgtatg tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt
     5041 gtgagagggg ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac
     5101 taattgaaaa gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact
     5161 gaaaatcgac aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat
     5221 aacgtccgca tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt
     5281 ttttttcttg tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt
     5341 gccaattata ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag
     5401 tatagtaaga tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa
     5461 cttatactag ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat
     5521 tcaaggtata gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat
     5581 cttggttcag gaaaaacgat acttctaaat catattctgt acggtgatca cggaaagaaa
     5641 attgctgtca ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag
     5701 aaaggtttta aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt
     5761 acattacgcg aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat
     5821 caaattgtta ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata
     5881 ttagctaaaa accctttaga cattgatttg acccaacgat tagagattgg tgccattatt
     5941 atagtagtgg atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa
     6001 agaaaagctg aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat
     6061 caattcgagt gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg
     6121 atttacaaaa ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca
     6181 attaaaggtg aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag
     6241 acttcctatt cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct
     6301 gaaacagaag aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca
     6361 caaaaatttg cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg
     6421 tttctttggt ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat
     6481 aaaagtattg aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg
     6541 attatacaaa aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt
     6601 aataaactag tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag
     6661 ggttgtttaa ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg
     6721 aaaaagtcta atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc
     6781 aagataggcg atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt
     6841 gtattctcta tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt
     6901 cgaattgatg gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg
     6961 aattatcatc ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga
     7021 gttaaagaag ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc
     7081 ggtcaaacga gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc
     7141 acagattact acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat
     7201 attattatta ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa
     7261 agaagcggtg acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag
     7321 tcggttagat tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga
     7381 ataatcgaga tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc
     7441 tttagtcagt tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat
     7501 tcgttctcgg tagttttgaa agtttctata gccataggca attcgtttta gtactttgat
     7561 atggttattc aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat
     7621 ttctgtttgg tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt
     7681 gtagtcttct tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag
     7741 aaaatcctgg tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc
     7801 gactatctct gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc
     7861 tttcaattcc cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg
     7921 acgatcccct tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc
     7981 aatgtgttgg ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt
     8041 caccaatgtg cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac
     8101 ttttataggg tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat
     8161 atcaatcagt tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt
     8221 tttcactgat ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat
     8281 ggttttaggc cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat
     8341 acgtgcgatc aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac
     8401 tctgcgagca atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc
     8461 acagtgttta catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac
     8521 atcattcaat aagatggata cttttttcca gccctattta atgatcgttt gttcattttg
     8581 atagtggcga tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca
     8641 tgtttcgtca ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc
     8701 tagaatatct agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga
     8761 atggttttcg tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa
     8821 aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt
     8881 ttcttcgctt caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg
     8941 tagtagtctt ctaaataatc ttggattaac accttcatct ctattttact tttatatttg
     9001 ttggagtcga ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca
     9061 acaggagtcc cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga
     9121 taaataaaag accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct
     9181 taataaattt gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag
     9241 taacgtagaa tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta
     9301 catggaatgt aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat
     9361 cgattcaaca tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc
     9421 gtcgatgaaa tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt
     9481 tattgatttc gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat
     9541 ctttgtgttc gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat
     9601 gtagaaatct gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta
     9661 tcgacgatat tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc
     9721 aattttcttg gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt
     9781 acgatcatat gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag
     9841 aaaagtgtat catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat
     9901 agataagagt ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg
     9961 attggacaca tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca
    10021 attctagagg agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc
    10081 ccacttcata cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa
    10141 gctaaaaacg aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc
    10201 attatagaac gctgtaaaat
//
LOCUS       DADVXN010000098        10077 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.101, whole genome shotgun sequence.
ACCESSION   DADVXN010000098 DADVXN010000000
VERSION     DADVXN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.101"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(75..227)
                     /locus_tag="KB942_002251"
     CDS             complement(75..227)
                     /locus_tag="KB942_002251"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6852641.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(418..1296)
                     /locus_tag="KB942_002252"
     CDS             complement(418..1296)
                     /locus_tag="KB942_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6852642.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1335..2453)
                     /locus_tag="KB942_002253"
     CDS             complement(1335..2453)
                     /locus_tag="KB942_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6852643.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2450..4549)
                     /locus_tag="KB942_002254"
     CDS             complement(2450..4549)
                     /locus_tag="KB942_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBB6852644.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4600..5421)
                     /locus_tag="KB942_002255"
     CDS             complement(4600..5421)
                     /locus_tag="KB942_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6852645.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5418..6227)
                     /locus_tag="KB942_002256"
     CDS             complement(5418..6227)
                     /locus_tag="KB942_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852646.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6248..6742)
                     /locus_tag="KB942_002257"
     CDS             complement(6248..6742)
                     /locus_tag="KB942_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852647.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6754..7242)
                     /locus_tag="KB942_002258"
     CDS             complement(6754..7242)
                     /locus_tag="KB942_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852648.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7319..10015)
                     /locus_tag="KB942_002259"
     CDS             complement(7319..10015)
                     /locus_tag="KB942_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6852649.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3063 a         2271 c         1657 g         3086 t
ORIGIN      
        1 ttcttgctat ctttttataa tcacaactct ctataataga attacgattt attttatacg
       61 gctgaaaaaa tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca
      121 tttcgaaaaa tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa
      181 cacaatatat tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca
      241 caacaaaaag aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac
      301 acgatttgac ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga
      361 tcatttgatt actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta
      421 tggttgctta tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt
      481 atcacctaat tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt
      541 tataagctca acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc
      601 accgccaaaa ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc
      661 aaggtaaaag gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac
      721 acccaaccgt ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa
      781 gggacagttt cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc
      841 cggatgactg aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt
      901 tcccacagtg aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc
      961 atagcaagcc gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag
     1021 cgttcctaca aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa
     1081 actcgttgat ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa
     1141 ttgttcctta aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt
     1201 tgtgggaaaa ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa
     1261 tttagtccct ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg
     1321 tgaaaaacac ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt
     1381 cctgaagctt ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat
     1441 aagcgaacat catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag
     1501 atggggtctt gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag
     1561 gcatgaacgg cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat
     1621 tggtaaggat accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca
     1681 tcgtactcat gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg
     1741 gtcggatact cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa
     1801 cgttctaggc aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga
     1861 tcaattaact tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga
     1921 aaatataggc tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat
     1981 gaggacatat ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc
     2041 atttcgaaat aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc
     2101 tcgcaacgct ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg
     2161 ctatccgacc catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat
     2221 tctgttcgat cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc
     2281 gcaatcacag tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt
     2341 cctagctcgc ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg
     2401 attgctcgac tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc
     2461 tcctttgaat gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta
     2521 ttttcaatgg cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg
     2581 aaatgaaggc ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat
     2641 caatttgcaa tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga
     2701 caaaaagacc cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt
     2761 gttgagcctc taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa
     2821 ttagttcttc taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag
     2881 tgataaagcc aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct
     2941 cgctataggt tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat
     3001 ctttattatc taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat
     3061 gcatcaataa attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct
     3121 caagaatcgg tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc
     3181 cctccaaaaa ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct
     3241 ttagtttaca ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa
     3301 aaatttctgc aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact
     3361 ttccaagtgc aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc
     3421 gacgttgagc taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc
     3481 gctcaaagaa ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg
     3541 ataacgaatt actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa
     3601 cgccatattg atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga
     3661 atggcaatga tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc
     3721 cccaattgct gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat
     3781 aaagtgcacg acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata
     3841 gctgagcaaa atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag
     3901 tcattacttc ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct
     3961 gttgtgcgat cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat
     4021 acttctcttg atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat
     4081 tcacaatgta aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa
     4141 aggatactac aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt
     4201 gaatcacatc gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta
     4261 cttcaacctt taaggataac gaattggttt gagagagctg tttattgacc caaacacctg
     4321 gtttttcctc agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa
     4381 catcaaggtt tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag
     4441 gaatattccg atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag
     4501 cattaaaaaa tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc
     4561 ttgctaaaaa aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga
     4621 caataaaatt cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc
     4681 tagtaatttg aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc
     4741 gaggatttct tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt
     4801 gaatttgacc atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc
     4861 ttttgtcaga atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa
     4921 tgaatagcct aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac
     4981 taaaaagata attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc
     5041 ggtaacgaca cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat
     5101 caaactcgtt ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc
     5161 cttctccatc gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa
     5221 atactgtgta tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt
     5281 aatgaagggc atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga
     5341 agcatacata gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga
     5401 aattccatca gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt
     5461 acagcagcat cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg
     5521 gctataacaa ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga
     5581 tacgccataa caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc
     5641 cgtaacagca tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc
     5701 atgatgacct ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg
     5761 gcaaagaatg gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga
     5821 ttgatgattc ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt
     5881 ccacatacaa aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag
     5941 acaatgggaa taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc
     6001 ccagcagcca aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag
     6061 atggcttcta gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct
     6121 gctaacggag caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca
     6181 gcaactccaa taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt
     6241 cctttttcta aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag
     6301 gctgaaaaac ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg
     6361 tcgttaacat cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc
     6421 cagcattccc cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg
     6481 catctttgac attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta
     6541 acaaatgaat cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca
     6601 tcttcaatgt catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc
     6661 cggttaaatt tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat
     6721 caattcgcac tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac
     6781 tacatgtacc tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc
     6841 ctgaatatcc tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag
     6901 attaaagcca gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt
     6961 ctgattgaca cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac
     7021 ctggtttaca aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat
     7081 caccgtaatc tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc
     7141 taagtgaccg tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt
     7201 cacttttata taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat
     7261 tcacattaaa aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt
     7321 atttttgaat atacggtcct aataattcat agagcagcga aagctcataa gcattgactt
     7381 taaaattata tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat
     7441 cttcatctgg tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata
     7501 aacgttccat catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag
     7561 tgaaatgata gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg
     7621 ttgggttcag aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct
     7681 cctgaatcaa gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat
     7741 tggctgctcc atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac
     7801 gtggaagatc cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt
     7861 ggtcgattaa ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg
     7921 ataaatgatg acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg
     7981 aaatgataat gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact
     8041 tgtctgctgc atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa
     8101 tatcaagtgc aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt
     8161 tcttgatctg tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat
     8221 ctgtattcac ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac
     8281 tctctactgg catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg
     8341 cttgaatgct gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg
     8401 attcatctac ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct
     8461 tttctgagag cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta
     8521 gatttgcagc taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga
     8581 cataggatct gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc
     8641 ctaaaacgga tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata
     8701 aaaaagaatc cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa
     8761 aattttcatt gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat
     8821 aatcaaacgg tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag
     8881 caatcggcaa gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt
     8941 tcccaatcgt taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa
     9001 tgatattctt cagcttacca atattcccac taaagggatg agataacatt aaagacaagg
     9061 cttctcttga aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt
     9121 gaatcagctc taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa
     9181 tttgaatacg ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga
     9241 aaataaaacg aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc
     9301 cagtatcaat aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat
     9361 cgagaaaaag atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc
     9421 ccgtaaaggc ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg
     9481 cataatcggc acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga
     9541 tagcgtattc aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta
     9601 ttaatacact gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct
     9661 ctctcagact tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat
     9721 tatctacaac cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct
     9781 cgaccttttt atcttccact aatctcgtca aataatggct ggcattctga cgagagatac
     9841 ctagattctt cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca
     9901 gctcatcaaa aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc
     9961 taaaagcata ataaactatc ggctgcaata tgtctattct atatttgtat tccaattttt
    10021 actttgaaac acaaatagtc gaatatttgc ctcagatatc tgacaaaata atttatc
//
LOCUS       DADVXN010000099         9949 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.102, whole genome shotgun sequence.
ACCESSION   DADVXN010000099 DADVXN010000000
VERSION     DADVXN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.102"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            32..388
                     /locus_tag="KB942_002260"
     CDS             32..388
                     /locus_tag="KB942_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBB6852650.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="KB942_002261"
     CDS             404..754
                     /locus_tag="KB942_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBB6852651.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="KB942_002262"
     CDS             783..1562
                     /locus_tag="KB942_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBB6852652.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="KB942_002263"
     CDS             1573..2235
                     /locus_tag="KB942_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBB6852653.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="KB942_002264"
     CDS             2254..3360
                     /locus_tag="KB942_002264"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBB6852654.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="KB942_002265"
     CDS             3350..4441
                     /locus_tag="KB942_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBB6852655.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="KB942_002266"
     CDS             4454..5194
                     /locus_tag="KB942_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBB6852656.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="KB942_002267"
     CDS             5482..7422
                     /locus_tag="KB942_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBB6852657.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="KB942_002268"
     CDS             7509..9431
                     /locus_tag="KB942_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBB6852658.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9949)
                     /locus_tag="KB942_002269"
     CDS             complement(9711..>9949)
                     /locus_tag="KB942_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6852659.1"
                     /translation="SDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPA
                     QAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3249 a         1652 c         2209 g         2839 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaatca aatcagacg
//
LOCUS       DADVXN010000100         9886 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.103, whole genome shotgun sequence.
ACCESSION   DADVXN010000100 DADVXN010000000
VERSION     DADVXN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.103"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(109..2229)
                     /locus_tag="KB942_002270"
     CDS             complement(109..2229)
                     /locus_tag="KB942_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6852660.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="KB942_002271"
     CDS             2459..3637
                     /locus_tag="KB942_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBB6852661.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="KB942_002272"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="KB942_002272"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBB6852662.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="KB942_002273"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="KB942_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBB6852663.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="KB942_002274"
     CDS             complement(4990..6243)
                     /locus_tag="KB942_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6852664.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="KB942_002275"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="KB942_002275"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBB6852665.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="KB942_002276"
     CDS             7821..9167
                     /locus_tag="KB942_002276"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBB6852666.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="KB942_002277"
     CDS             complement(9475..9861)
                     /locus_tag="KB942_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6852667.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3149 a         1800 c         2024 g         2913 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DADVXN010000101         9758 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.104, whole genome shotgun sequence.
ACCESSION   DADVXN010000101 DADVXN010000000
VERSION     DADVXN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.104"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            56..3334
                     /locus_tag="KB942_002278"
     CDS             56..3334
                     /locus_tag="KB942_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBB6852668.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3875..5782)
                     /locus_tag="KB942_002279"
     CDS             complement(3875..5782)
                     /locus_tag="KB942_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBB6852669.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5912..6787
                     /locus_tag="KB942_002280"
     CDS             5912..6787
                     /locus_tag="KB942_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBB6852670.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6867..7841
                     /locus_tag="KB942_002281"
     CDS             6867..7841
                     /locus_tag="KB942_002281"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6852671.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8021..9166
                     /locus_tag="KB942_002282"
     CDS             8021..9166
                     /locus_tag="KB942_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6852672.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9303..9647
                     /locus_tag="KB942_002283"
     CDS             9303..9647
                     /locus_tag="KB942_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBB6852673.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3179 a         1801 c         2061 g         2717 t
ORIGIN      
        1 aaattaggta atactttaag taaacgcttt ctaatttgtt acgggaggaa tacacttgtt
       61 aaaaaaacgt ataaaataca gtgcgctaat gatgaatctc cttatgctca gtaccccggt
      121 tttaatttct gtcgttgaag ttgctgctaa cgaacaacag tctgtgaacg aagaaaatgc
      181 acacgtgaac attgaaggga tgtcaacaat ggaggaaata ccaagaacta gtgaagatat
      241 ggatacggaa gaaggaaatg atcctatgat gaaagaatca gaaaatgaaa agacaaaaaa
      301 gcaatctgaa gaagcaaatg aagagtggaa aggggttgtt ttcggagaaa gcacaagtgc
      361 tagtaggtat gccattgaac cattagaaga cggcgtgcgg ctctcttcca cgaataacgg
      421 cggaaaattc cagtcaagcg gatcagatgg aatgacctat tattatcaag caatccctaa
      481 agacatcaac tttaggatgc gggcgacgat cgaaatagaa tcttggacct atacgaatgc
      541 ccaagaagga tttgctttaa tggtaagaga cgcagtacca aaaaatcatt tgtttgggca
      601 ggcattttat tcaaattcat ttgccatact aggcagtcgg atcgaatatg tctgggactc
      661 ggatagacaa gaagtggtca acacgagcgg cagcaaatat acgatgcgac ttggattggg
      721 gacacgggca atcacaggaa tatcatctga agatccgcaa gctgcgccag ctcctgggag
      781 tgtctcagtg gaaacaacac ctttagagac ttcagcaggg ttcttggaaa aagaaacagg
      841 ttcttataat atattcggaa acggtcatgg gaatctgttt ccggccacga actcgattac
      901 taaactggac gtagagatga aaaaaacgaa tactggttta gaggcatatt attacgatcc
      961 tgaaacagga gaagaaatgg cttctgacat catgtatgac tgggaaaaat tatacgcttc
     1021 agatgaatcg gttatttatg caggatttgc tgcagcaagg aatatgacga tcaaggtaga
     1081 aggaatcgaa gttgcttact cagatcctgc gacagatcct cctgggcaag aaagacctac
     1141 tcgcttgatt gatccgattt acactgtgac ctctagtaat cattcaggaa accaagatca
     1201 tactatgtcc tttagagcaa atgctgacgg attattgaca attaaaaata aagcaacagg
     1261 agaggtgctt aagaacgcaa aaaacttggc tatcactgcg aatcgggaat ttgattatca
     1321 gacaaaactg atggaaggaa cgaacgaatt tactttcagt tttacgccag ataaaaacta
     1381 tccgccagaa gaatacgtag agatgactag ttatgaaaca aaagagattc ttcatacagt
     1441 ggactaccgt aaacctaaat attcgacagt ttatgtaaca ccagaagggt cagatgcagg
     1501 aaacggaagc agacaaaatc cattatctct gacacaagca ttgagatacg catatgctgg
     1561 gcagacaatc gtcatggcgc ctggaactta ttccttcgaa agaggattga cgattccaaa
     1621 aggcgttaac gggacaagag aaaacccgat tcagttgatt gcagagaaaa atccagaaaa
     1681 caagcgtcct gtacttgatt ttaaaggaac tggaaatggg atgacactgt tcagtcacta
     1741 ctggggttta cgagggtttg acattacaaa tagtgcagct atgcaaaaag gattacagat
     1801 ttctggaaat cataatttga tcgaagagat tcatgcttat cggaatggaa atacaggaat
     1861 ccaaatcagc ggtagctcaa atgatccttt cgaagcatgg cccgcgcata acttagtcaa
     1921 gaactgtaca tcttttgaaa atgccgatcc aggatttgaa gatgccgatg gatttgcggc
     1981 caaactgaca ataggagaag gaaatgtatt tgacggttgt atcgcgtatc ataatgcaga
     2041 tgacggttgg gacctttttg ctaaaaatga aacaggttcg atcggaaaag taatcatcaa
     2101 aaactcagtt gcttatcgaa acggctgggt accaggaatt gagggagaag gtaacggtaa
     2161 tggattcaaa atgggcggtt cttctttaac gggccctcat gaattgatca atagtttgtc
     2221 tttcgaaaat cttgcgaaag gaatcgattc caatagcgga acagatatta tcgtcaatag
     2281 cagtacaagt tttaataacg gttcatataa tgtggctctt tatacctctt cagcaggaaa
     2341 cacagactac catgcttctg gtatcttatc tttccgaaca gagaatcttg agatgagaga
     2401 gcagatccgc ccattgaatc aaaacgaaga tcaaatctac cactcgttga actactattg
     2461 gaatgaacga gagaaacaat cagaaaatac tcttggacat accatatcaa aagattggat
     2521 cgagtctcta aatacatgtt cgaaagaagg tcagcagcct gttacgcgct atgaaaatgg
     2581 aagtatccat gttcatggac tgatgcagat taaaccgggg aatcgtcaaa cagaaaagga
     2641 aagagtggat ggtcttccat tatctgtagg tgctattttt gacggagaaa cctcaccgtc
     2701 ttcggaatat ccagagtatg caatcgtaca agaaccaaat cagacaaaac caaatgaaca
     2761 aaataatccg attattatac aagaagcaaa tggtgtggcg gagaatgcag aaggaaatat
     2821 cattgaactg aagcctttta cagtttttgt gtcaactttc acaccaaatc aaggaacgcc
     2881 tgtctttgaa gatatgcgtt atttcccaga aaagatccaa atcgttgaaa cacagtcaag
     2941 tgctcaattt tctaatcata ccgctatttt ttcagcacct tcaaaaggag aatatacgct
     3001 agaagtcact tatcgttcgg aaatatttga tggggaaaaa tggtctgctc atacagaatc
     3061 tgttacccag caaaaagcga tcactgtgcg agaaactgcc gatgtgcccc cgggaaatga
     3121 caaaccagaa agagaaaatc caggaaatga atcaccaaat ggaaaaccaa atggaaaacc
     3181 ttctcatccg gataggacag caaaagaaag tgaaaaaaat ggcactcaag gactcccaaa
     3241 aacgggagaa tttaataacc cgttgctagc attagctgga ggaattttgt taatcggtgt
     3301 agttgtttat gtcatgaaac agcgaaaaaa ataagtggaa tttctccttt tatgatgtaa
     3361 aacaattgac tgaggaaagt aaaaatagaa ggaaatgaga ccaatagatg tctcacttcc
     3421 ttctatcttt ttatagatga ggttctcctt gctgcttttg attggctgtt cttattactt
     3481 gatgcaggac agctttttca caatctctcc actaaacggt gttcaatcag ctgaccctcg
     3541 gtattagctc aacaaacggc aaaatatgag gaactatttt tccgtttttt tctctaatac
     3601 tcaggtcagg acgctgattt cacaacctct cgataaacgg tgttcaaaag ccagtttctc
     3661 ggtagtaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttact
     3721 acttgaaact aagcagcttt ttcacaacct ctaatacatg gtattccaga agtaacttct
     3781 tcgaaaaata agccgaaatg tcacaaaaat ttgagaagcg attttcccaa attccttctt
     3841 atttcttgaa gctgaccact tctggcacaa cctcttacca gattcgcact cttttttctg
     3901 gcgctagata catagcgtct tgttccgtga taccaaatgt ttcatagaat tcgtcaaggt
     3961 tttttggttg cacgtttgct cggagtttag caggggcatg gacatcgatt tgcagtaaaa
     4021 gttgttggta ttcttttttt gcttttgtac gccaaatcgt tgcccaattg acaaagaatt
     4081 cctcgataga aagatctgat tccgtttttg ctgcttccaa tgcacagctt agaccgcctg
     4141 catcagcaat attttccgaa acggttaatt ttccgtttac ttttccgtca gcaaaaggca
     4201 gaccatcgaa ttcttcgatc attgcttgtg ctttgttttg aaattgtgta agatcttctt
     4261 ctgtccacca gttgttcaag ttgccatatt cgtcaaataa agcgccgtta ttatcaaatg
     4321 catgggaaat ctcatgagcg ataacagctc cgattccacc ataattagca gagcttgatt
     4381 gttctagact gtaaaatggt gcttgaagga tcgctgccgg aaagacgatc acattccgaa
     4441 atggatgata ataagcattg accgtgtcag cactcatttc ccattctgaa cgatccactg
     4501 gtttgttcca tttgctgaaa cgatctttta atgtgagttt gctgaaaaca agagtatttg
     4561 aaagcaaagt accaccctga tctgcaggta ctgttttgaa ctggtcgaat aatgctggga
     4621 tcttatcggg gtagcctact tggataccta agttatttaa ttttatgacg gctttttctt
     4681 ttgttgcatc cccaagccag tcattttctt ctagacgttt tttataaacg gcgatcattt
     4741 ttttcaccat ttgttctacg tcttttttag cagcttcgcc aaaatatttt tttccgtagt
     4801 aatcgccaac tacttgatcg aattgtccag aggcaaggta atacgctgct tttttctgag
     4861 gcattgcttc gtctgttcca gacaatacgc gagaatagat tccgctgact tggcggaatt
     4921 cttctgatag ataaccacta agagaacgta ctgttttaat gatcatccag cttttgaata
     4981 acggaaaatg agcttctgtc aggatgtcat tcaaatgatc gaagtacact ggatcagtta
     5041 cgatcacttt atctggagtt gtcccaatca aacccgtgac aagttttttc aagtccagtt
     5101 tatctgtgta tttaataaat gtatcaaaag attgcggatt atacattttg ctgtaatctg
     5161 cgttttcttc tgcacttttt acatgaggag caatcaattt atcaaattgc actgcttgtt
     5221 ccgtgattgc ttctgcctga cttttttctt tgccggcaag ctctaataac tgaacagcca
     5281 tatcaaagaa aacgcctatt agttgtgcac cgttgggatg gtctgatgca taatacgtct
     5341 tatctggaag gaataaagaa ggcggataag caaagaaggc gttcgtttgc gcattcttca
     5401 tatctgcatc tacatccaga ttaaatggca atggtaatgt atccaacagc cattctggaa
     5461 attgcttatt caattcgtcg aaagactgca atgattcaac tttttcgatt ataggttgaa
     5521 gcggttgagc atgcaactca tttcttcggt cataatcgtt tgccagttga taatatgcaa
     5581 ggaaatgttt cattttttct gaagacactt tttctggatg ggcaagcaga tcgtctgttt
     5641 ctcccatcaa aagttggtcg atcccgtcaa ctaaatcttg gaaaccgcct gtagctggtt
     5701 tatctgcagg gatttttgct gttttttgcc attcttcatt gactgcttca tagaaatctt
     5761 gtttcaatcg ttctttgttc attcatcgat cgctcctttt ttgtttcgtg tgcccattat
     5821 cttacaaaat gaaggaatta acaaatagaa cataaaaaat tgaaaattcg aacaatcaaa
     5881 ctgatttgtt tttttcaatc cttcacttat aatgaaaaaa aggaggcgat cccaactgaa
     5941 tcaagtagtg gataaacctt tatatttcaa agtagataaa aaaattaaaa aattagccag
     6001 cactcagcag ctgcagtcaa gaaaaagtaa attgcttttt ttagctctgg tttttgaaga
     6061 tcaaagttat gtgattatcg atcaatcagg acatccggta gaatattctc cagcaaaata
     6121 tacttatcaa gagggtcttt cttgtaaaaa atggaagctg atcaatgaag agccaatcga
     6181 gttatctaga tggatcaaca gaaaagaaga tattcccgtt ttgatcgaag aaaaaaggtc
     6241 aggaaaggaa ctcgcaaatt gctgggtggg attgccagaa gaacgttttt tgaggtataa
     6301 gaaatgggct accccctcag gctatctttg tggtacttac gcggcagcag ttctccttgc
     6361 ctattatcaa gactatcgta aggggtggat gcttccaaat gagattcgca agaaaaatac
     6421 ggctgattcg ttggttttga cgaaggcatt gcgtagccag atccaaccac taggacttcc
     6481 tacgattcct tttcaggtta gtacagggat ttctagtttt ttaaagaaga atggcaatca
     6541 tgagcgtgca cgagcaactc ttctaggttc atggcagcga gctacaaaac gtatacgtga
     6601 aggcaaacct gtgatgattg gtattttgaa agtacttggc agtacatatg gcaatcattg
     6661 ggtgacagcg tacgcttatt ttgaaactga aacaggagaa cgttattaca aagtacacga
     6721 caactggggc gattatcata aagtcattcc tgccagttgg agtaacggaa cagtttcact
     6781 tccttaagaa tttgctgaaa ttaacagaaa atcttggaaa aatatgttaa actatcgaag
     6841 aatagagaag ttttggggag gactacatga gtaataagta tcaggatgac acgttgaaga
     6901 tttttagttt gaatggcaac cgtccattag ctgaaaaaat cgcgaaagtg ttcggaacag
     6961 aattaggtaa aagcgtcgtg aagcaattta gtgatggcga gatttccatc aatatcgaag
     7021 aaagtattcg aggggatcat gtgtatatcg ttcaatcaac gaatgcacct gtcaatgatt
     7081 actatatgga actactgatc atgatcgatg caatgaagcg agcaagtgcg aaaacgatca
     7141 atgtcgtatt gccttactat gggtatgcta ggcaagaccg cacggcgaaa cctcatgaac
     7201 cgatcactgc gaaattgatc gctaacttga tcgaagaagc aggggcaact cgagtactga
     7261 ctttagatct gcacacggtt caagtacaag gattctttga tattcctgta gataatttat
     7321 ttacgatgcc gttgtttgct cattattacc gtcaattagg tctgacggga gatgacatcg
     7381 tagttgtttc gccaaaaaac agcggtgttc agcgtgcacg tagtctttca gaatatttga
     7441 acagcacatt agccatcgta gatcatgcag atgatgcttc aactgagggt ggttatgtga
     7501 tcggtgatgt tcaaggaaag acatgtatca tggtagatga tatcttgaac acaggagcaa
     7561 cgcttgcacg agcagcaaat gtattaaaag aaaacggcgc aaaagaagta tatgcctgtg
     7621 cgtcacatgg attattgtcc gatcatgcga aaagtatttt agacaatgca ccaatcaaag
     7681 atatttgtat cactgattct gttttcacag aagaaaatcg tcatccagaa aacttaacaa
     7741 tcgttacatg ttcagaatta atgggtgaag cggtcaaacg catccatgaa aatacaccga
     7801 tgagtccgtt attccgtctg gaagaaaaag aattcgaata aacaaaaaaa agagggaaag
     7861 aagttgtgac agaagtgagc agcttcaagg agctgcgaca agacttttgt cacagctcct
     7921 tttttctatg cccatgaaac gtcctctaga caaaaatgag ctcttctttg gtttgttcgc
     7981 tattttatgc tagaatgtgc ctagcaataa tttggaaggg atgaatgaag tgaaaactat
     8041 ttatctggat catgcggcaa ctacccccat gcatccacaa gtaattgaag cgatgacggc
     8101 aatcatgcaa gacacgtttg gaaatccttc aagcatacat ggatttggtc gccacgcgca
     8161 tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa aacctcacga
     8221 gatcatcttt aacagtgggg gaacagaagg ggataataca gctatcttag aaacagcgtt
     8281 ctcgcgtcaa aaagaaggca gacatctaat cactacagct atcgaacatc ctgctgttct
     8341 taataccatg aaatatttag agacaaaggg gtttgacgtt acatatctgc ctgtagatga
     8401 aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata cgatcttggt
     8461 ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag aaatcggtga
     8521 acttttagcg gatcatccag cttatttcca tacagatgcg gtacaagcgt acggaaacca
     8581 aacgatccat ccagaagaat taggaatcga tttattaagt atctctgctc ataaaatcaa
     8641 tgggccaaaa ggcgtcggtt tcttatataa acgagacgga gtaaatatcc ctgcattgct
     8701 tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag cagggatctg
     8761 gggtatggca aaagcagttg aactgctgtc acctgaagaa cgtgaagcac gtaacaacaa
     8821 atataaagga ttccagcaat tgattcttga tacattagaa gcaaacgaaa tcccttatca
     8881 agtaaatggt gatttaacga ataaactccc tcatgtgttg aatcttcgct taattggcga
     8941 agcaaatgat ttgctactga tgaagcttga tcttaatggg gtcgctattt ctactggttc
     9001 agcttgtaca gcaggaaata tcgagccttc acacgtatta gaagcaatgt atggagaaga
     9061 tacaccgata ctaaaagaat ccatccgtat cagctttggt ttgggaaata ccgaagacga
     9121 catccagtat ttccttgatc aattagttcg tgctgtcaaa aaataactag tgaagtcatt
     9181 cttatatgaa cagatgtctg agagaacgcc ttcatttttt agggaactta actgaaaaac
     9241 ctagtaaaaa tcaggataaa cttttataat aaagagaaac taattttatc gaggtgaaaa
     9301 tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc aattagctat cgtttgcatc
     9361 ctgatgcaaa aagatataca ttgcgcgata atggatttac agaaacaaac ggaggcaatt
     9421 atcaattgat ccgaccatta gatgccacgc cgcaaagcaa agaaggattc aaattaaaaa
     9481 tcactgtatc aaaagatatt caaacattga aaatgtcgat cactacagca aatggattga
     9541 aagcagtcaa tattttcaaa gatccaaaac agaaaatgag ccaagataaa ttttattttc
     9601 tgatggatgg gatgattagc cgtgggttat tcgaaaaagt cgaatgatct gtggatataa
     9661 aaagacaact agttttgaaa cggcctattg ttagatgact aactttgtaa ggacgacgca
     9721 aattagttgt cttttttatt tttaaattta acacatgt
//
LOCUS       DADVXN010000102         9547 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.105, whole genome shotgun sequence.
ACCESSION   DADVXN010000102 DADVXN010000000
VERSION     DADVXN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.105"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            118..1107
                     /locus_tag="KB942_002284"
     CDS             118..1107
                     /locus_tag="KB942_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6852674.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="KB942_002285"
     CDS             1089..1550
                     /locus_tag="KB942_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6852675.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="KB942_002286"
     CDS             1600..2064
                     /locus_tag="KB942_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852676.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="KB942_002287"
     CDS             2078..2827
                     /locus_tag="KB942_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852677.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="KB942_002288"
     CDS             2827..3657
                     /locus_tag="KB942_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6852678.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="KB942_002289"
     CDS             3657..4304
                     /locus_tag="KB942_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6852679.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="KB942_002290"
     CDS             complement(4402..5178)
                     /locus_tag="KB942_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852680.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5322..6281
                     /locus_tag="KB942_002291"
     CDS             5322..6281
                     /locus_tag="KB942_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6852681.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6398..6706
                     /locus_tag="KB942_002292"
     CDS             6398..6706
                     /locus_tag="KB942_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6852682.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6733..7032
                     /locus_tag="KB942_002293"
     CDS             6733..7032
                     /locus_tag="KB942_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBB6852683.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7206..7757
                     /locus_tag="KB942_002294"
     CDS             7206..7757
                     /locus_tag="KB942_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBB6852684.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7903..8196
                     /locus_tag="KB942_002295"
     CDS             7903..8196
                     /locus_tag="KB942_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852685.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8189..8485
                     /locus_tag="KB942_002296"
     CDS             8189..8485
                     /locus_tag="KB942_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852686.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8556..>9547
                     /locus_tag="KB942_002297"
     CDS             8556..>9547
                     /locus_tag="KB942_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6852687.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNF"
BASE COUNT         3375 a         1369 c         1789 g         3014 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaaa tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tattgtaaag ttatatgact ttataatact tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtatcaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgcatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttaataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgacttca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgc taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtaaat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaacgt cgattgtaaa attaagctag acaactaaaa agatcatttg tgctacactc
     5281 aaaatagttc cgaccaaaga attaataagg agtgagtaca aatgacttac acccatctta
     5341 caacagacga actagtaatc atagagtctt atttcaatat cgagaaacct gtcgctcttg
     5401 tcgcacaaag tttaaatcgc tctagacaaa ccatttataa tgtttattcg tttctcaaac
     5461 aaggaaaaaa agtgctagat tattaccgtc aatacaaaca aaataaatct cgttgtggac
     5521 gacgttcaac cgttttatct gatgaacaat caacatatgt tcaagaaaag gtagctcaag
     5581 gatggtcacc cgatgtgatt atcggccgtg gcgagtttcc aattgattgt tctgttcgta
     5641 cactctatcg gatgtttaaa aaaggccttt ttgacacaca gtcgttacct atgaagggga
     5701 aacgtaaacc gaataatcat caagaaaagc gtggaaaaca ggcctttcgc agaacgattc
     5761 atgaacggaa cagtctctat ccacacctga ctaaagaatt tggtcatctc gaagtcgaca
     5821 ccattgtggg agttaaacac aagagcgctg ttattacatt ggttgagtgt ttatccaagg
     5881 ttattattac tttgaaaccg acaggcagac gagccgaaga tatcgaaaac aggttaaatg
     5941 attggttcaa atccatgcct aaacacttgt ttaaatccat gacatttgat tgtggaaaag
     6001 aattttccaa ctggcgatct ataagtaact cgaacgatat tgccatcttt ttcgcagatc
     6061 caggaacacc ttcgcaaaga gggctaaatg agcactcaaa cggcttgctt agacgagatg
     6121 gcttacctaa aacaatggac tttagggaca cggatgaaac ctttattcaa tccgttgcct
     6181 cgaaaagaaa ccatattcca agaaaatcat taaattatcg aacaccattg gaagtatttt
     6241 tgagctgcat agataaagac atattgtcta gcttaatttg acaaatcaga tagtataaaa
     6301 tgaatcaaat tgaatatcaa atatatatta tatccatgaa gcccaatctt ctttaataga
     6361 agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata aaggacaacg
     6421 ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt tatgttggaa
     6481 tctaatcgat caaaacgttc aaaagcaatt tcatttagac tatcttcaaa tctttgaatt
     6541 ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccag aagcaagaaa
     6601 agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa tatgggtcat
     6661 tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactgatatc taaaagaaag
     6721 gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc acagctagtc
     6781 actactcctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga aattgaaatg
     6841 ggtatgcttc ctgaagatgt tactctacca cgtttagtga tgacacatct ttatatttat
     6901 tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat taccagccag
     6961 cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca gattggagga
     7021 acgaatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag atatccttac
     7081 agcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt tcaactagta
     7141 ttgacactta taacggcaat ggtgattgaa ttgcttgaac aaaaattagc ataggagaaa
     7201 aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga gatcctcatc
     7261 atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa gcggaagata
     7321 aattagcggt agtcattact attcgtccaa cagatacaga accatttacg aatgatttga
     7381 gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt attgctgtga
     7441 atcttttttc gtatacaaaa gcgaaaagtc ctcgggtatt tcctcgtgga aatgatgaac
     7501 aatctttgga aatattaaca actgttttca cagaaaagaa aatcaacacc attatttttg
     7561 cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcgaag accatttacg
     7621 atcaattgag tgcgaaacaa aaaaagatga ccaaagtatt gataaatgca gaaggtaagt
     7681 tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat agtcaattat
     7741 tatttcaaga aaaataacat tttattttct tatgttactt ataagtagtt ttacatgctt
     7801 tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg tacccagtaa
     7861 tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat tgatgaaact
     7921 tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga actggattca
     7981 gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt tccaatgaaa
     8041 atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca ggaaaaaact
     8101 gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat tcaaaaagta
     8161 attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag gtggtttaat
     8221 ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag ataaaggaaa
     8281 agtaattaag acagttgggg taatcatagt tggactcata gctctaacta agaaaaataa
     8341 tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa gggaaatttc
     8401 tactagtttt aatccatctg aaagccctca aggaagagat cgttgtcatg ctggctatcg
     8461 acattcttgg gaaaggatta actaaatgaa caccattgag attaattgac tgataaaata
     8521 agtacgcgag ggagaaattg aaagggggaa ataaaatgaa aaaatggttg ttattactaa
     8581 ttggaatggg gattttattc actggttgta catctgagaa tacgaggact aaagagactg
     8641 tagaaactga tacaacaatt gaggctaaaa aaagtaaaac gcaatcaact gaacaaacta
     8701 caacagaaat gaaacaatct gtggaagcaa gtacaagtga aagttcaatc attgaatctt
     8761 cttctgaaga ggttaaaaat actttgtggg atagtgagaa atctactcaa ctagcaattt
     8821 ttgtcccaca atggggaaag acattaggtc aagaatataa aagctacaat cctcagaaca
     8881 atgtaagtct gtatggtacg cctttaccaa gtgcagttat tgatggaaat tggaaaatgg
     8941 caatcaatga agcaccagtt accgtacaat ggtcagaaga tggaactggt catgcagatt
     9001 tcaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg cagcatgtat
     9061 atttctttgg ctttcaaaat ggacaaccta aagtttattt aacgcaacaa aatcaaggca
     9121 atgataaaaa ctacatgtac ttcaatgaaa ctcagaacca acagttaaaa caaggtttca
     9181 ttgatattgt gaatggacaa actcctcaaa ctccagtagt agaagaatct actcaacaag
     9241 caacaactac aacgacacaa tctacaagta gctcagcaat ttcaagttgg gaagctgcta
     9301 aagaaactgt actaaataat aaagctttat ggttgtatga ttctcctgat aaaaatgcat
     9361 caaatactaa tttaattagc acaaatgaat atatgaaaga tctaatgcaa gatgaaaatg
     9421 gaagctacta ttcaattaat attgtaacgg atgataatgg aaatacaaca atggctggtg
     9481 gaaaacaatt ccgtgtttat acagatggaa gaatttctca acgtattgga atgactaaca
     9541 attttca
//
LOCUS       DADVXN010000103         9484 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.106, whole genome shotgun sequence.
ACCESSION   DADVXN010000103 DADVXN010000000
VERSION     DADVXN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.106"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            15..917
                     /gene="gnd"
                     /locus_tag="KB942_002298"
     CDS             15..917
                     /gene="gnd"
                     /locus_tag="KB942_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBB6852688.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            919..1920
                     /locus_tag="KB942_002299"
     CDS             919..1920
                     /locus_tag="KB942_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBB6852689.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1917..2201
                     /locus_tag="KB942_002300"
     CDS             1917..2201
                     /locus_tag="KB942_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBB6852690.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2234..3031
                     /locus_tag="KB942_002301"
     CDS             2234..3031
                     /locus_tag="KB942_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6852691.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3045..3878
                     /locus_tag="KB942_002302"
     CDS             3045..3878
                     /locus_tag="KB942_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6852692.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3896..4753
                     /locus_tag="KB942_002303"
     CDS             3896..4753
                     /locus_tag="KB942_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6852693.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4775..5284
                     /locus_tag="KB942_002304"
     CDS             4775..5284
                     /locus_tag="KB942_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852694.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5321..5722
                     /locus_tag="KB942_002305"
     CDS             5321..5722
                     /locus_tag="KB942_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852695.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5834..6187
                     /locus_tag="KB942_002306"
     CDS             5834..6187
                     /locus_tag="KB942_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852696.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6221..6664
                     /locus_tag="KB942_002307"
     CDS             6221..6664
                     /locus_tag="KB942_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6852697.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6681..7766
                     /gene="uxuA"
                     /locus_tag="KB942_002308"
     CDS             6681..7766
                     /gene="uxuA"
                     /locus_tag="KB942_002308"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6852698.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7759..8472
                     /locus_tag="KB942_002309"
     CDS             7759..8472
                     /locus_tag="KB942_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6852699.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8679..8828)
                     /locus_tag="KB942_002310"
     CDS             complement(8679..8828)
                     /locus_tag="KB942_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852700.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9224..9424
                     /locus_tag="KB942_002311"
     CDS             9224..9424
                     /locus_tag="KB942_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6852701.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3183 a         1495 c         2107 g         2699 t
ORIGIN      
        1 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
       61 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      121 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      181 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      241 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      301 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      361 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      421 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      481 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      541 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      601 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      661 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      721 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      781 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      841 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      901 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
      961 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1021 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1081 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1141 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1201 caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat
     1261 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1321 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1381 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1441 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1501 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1561 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1621 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1681 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1741 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1801 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1861 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1921 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     1981 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2041 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2101 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2161 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2221 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2281 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2341 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2401 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2461 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2521 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2581 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2641 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
     2701 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2761 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2821 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2881 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     2941 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3001 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3061 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3121 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3181 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3241 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3301 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3361 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3421 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3481 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3541 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3601 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3661 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3721 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3781 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3841 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3901 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     3961 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4021 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4081 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4141 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4201 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4261 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4321 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4381 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4441 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
     4501 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4561 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4621 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4681 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4741 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4801 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4861 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4921 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     4981 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5041 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5101 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5161 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5221 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5281 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5341 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5401 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5461 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5521 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5581 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5641 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5701 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5761 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5821 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5881 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     5941 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6001 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6061 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6121 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6181 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6241 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6301 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6361 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6421 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6481 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6541 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6601 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6661 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6721 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6781 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6841 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6901 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     6961 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7021 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7081 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7141 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7201 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7261 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7321 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7381 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7441 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7501 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7561 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7621 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7681 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7741 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7801 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7861 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7921 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     7981 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8041 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8101 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8161 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8221 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8281 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8341 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8401 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8461 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8521 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8581 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8641 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8701 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8761 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8821 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8881 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     8941 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9001 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9061 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9121 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9181 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9241 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9301 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9361 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9421 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
     9481 aaat
//
LOCUS       DADVXN010000104         9291 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.107, whole genome shotgun sequence.
ACCESSION   DADVXN010000104 DADVXN010000000
VERSION     DADVXN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.107"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(22..690)
                     /locus_tag="KB942_002312"
     CDS             complement(22..690)
                     /locus_tag="KB942_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6852702.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            824..1111
                     /locus_tag="KB942_002313"
     CDS             824..1111
                     /locus_tag="KB942_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852703.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1527..3065
                     /locus_tag="KB942_002314"
     CDS             1527..3065
                     /locus_tag="KB942_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBB6852704.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3062..4027
                     /locus_tag="KB942_002315"
     CDS             3062..4027
                     /locus_tag="KB942_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852705.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4014..5336
                     /locus_tag="KB942_002316"
     CDS             4014..5336
                     /locus_tag="KB942_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6852706.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5399..5503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5577..6320
                     /locus_tag="KB942_002317"
     CDS             5577..6320
                     /locus_tag="KB942_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852707.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6335..7327
                     /locus_tag="KB942_002318"
     CDS             6335..7327
                     /locus_tag="KB942_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852708.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7339..8076
                     /locus_tag="KB942_002319"
     CDS             7339..8076
                     /locus_tag="KB942_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852709.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8066..8788
                     /locus_tag="KB942_002320"
     CDS             8066..8788
                     /locus_tag="KB942_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6852710.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8872..>9291)
                     /locus_tag="KB942_002321"
     CDS             complement(8872..>9291)
                     /locus_tag="KB942_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6852711.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3042 a         1603 c         1965 g         2681 t
ORIGIN      
        1 taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa
       61 tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt
      121 cactttattc aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt
      181 gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt
      241 gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt
      301 gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa
      361 actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa
      421 aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag
      481 aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa
      541 tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga
      601 tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca
      661 caattctgta aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca
      721 gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta
      781 tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt
      841 attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca
      901 agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt
      961 tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa
     1021 gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt
     1081 gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga
     1141 tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt
     1201 cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc
     1261 tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt
     1321 tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact
     1381 ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt
     1441 cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact
     1501 taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa
     1561 aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga
     1621 ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag
     1681 aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt
     1741 tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg
     1801 ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg
     1861 aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc
     1921 ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta
     1981 cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta
     2041 ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa
     2101 ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta
     2161 ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta
     2221 aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa
     2281 tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg
     2341 gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt
     2401 tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt
     2461 tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga
     2521 cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg
     2581 cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag
     2641 cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat
     2701 atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca
     2761 ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg
     2821 atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga
     2881 tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg
     2941 gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa
     3001 agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc
     3061 agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat
     3121 cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg
     3181 tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga
     3241 catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat
     3301 gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga
     3361 agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact
     3421 tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc
     3481 tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat
     3541 tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt
     3601 ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga
     3661 agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg
     3721 tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga
     3781 tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt
     3841 aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt
     3901 atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg
     3961 gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa
     4021 catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga
     4081 aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta
     4141 accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa
     4201 aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa
     4261 aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag
     4321 ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca
     4381 acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct
     4441 taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac
     4501 tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg
     4561 ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat
     4621 ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg
     4681 cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa
     4741 aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag
     4801 aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg
     4861 attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa
     4921 ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg
     4981 atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta
     5041 aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga
     5101 tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat
     5161 tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg
     5221 aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata
     5281 gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag
     5341 catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata
     5401 cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa
     5461 cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg
     5521 tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga
     5581 agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg
     5641 tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat
     5701 tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc
     5761 ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga
     5821 agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg
     5881 tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg
     5941 ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat
     6001 caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa
     6061 tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt
     6121 attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt
     6181 acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg
     6241 tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc
     6301 gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc
     6361 gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat
     6421 aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg
     6481 catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat
     6541 cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg
     6601 tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa
     6661 agcaattgct acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa
     6721 catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga
     6781 agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat
     6841 ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta
     6901 ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc
     6961 attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca
     7021 actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta
     7081 tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga
     7141 aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga
     7201 tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca
     7261 ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat
     7321 aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac
     7381 ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc
     7441 gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg
     7501 acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt
     7561 tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta
     7621 tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc
     7681 tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag
     7741 cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca
     7801 tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct
     7861 aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta
     7921 tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg
     7981 cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt
     8041 tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag
     8101 tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc
     8161 gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta
     8221 cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa
     8281 aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac
     8341 tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt
     8401 tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa
     8461 tcactatttg atccattggt attcttcttt ggactatcaa caagactata ttgatttagg
     8521 ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa
     8581 agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc
     8641 tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac
     8701 aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt
     8761 ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct
     8821 ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca
     8881 accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata
     8941 ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc
     9001 taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt
     9061 ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac
     9121 agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc
     9181 ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc
     9241 agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DADVXN010000105         8787 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.108, whole genome shotgun sequence.
ACCESSION   DADVXN010000105 DADVXN010000000
VERSION     DADVXN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.108"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            7..306
                     /locus_tag="KB942_002322"
     CDS             7..306
                     /locus_tag="KB942_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852712.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(403..837)
                     /locus_tag="KB942_002323"
     CDS             complement(403..837)
                     /locus_tag="KB942_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852713.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1069..1779)
                     /locus_tag="KB942_002324"
     CDS             complement(1069..1779)
                     /locus_tag="KB942_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6852714.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1769..2629)
                     /locus_tag="KB942_002325"
     CDS             complement(1769..2629)
                     /locus_tag="KB942_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBB6852715.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2634..3272)
                     /locus_tag="KB942_002326"
     CDS             complement(2634..3272)
                     /locus_tag="KB942_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBB6852716.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3287..3586)
                     /locus_tag="KB942_002327"
     CDS             complement(3287..3586)
                     /locus_tag="KB942_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6852717.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3629..5113)
                     /locus_tag="KB942_002328"
     CDS             complement(3629..5113)
                     /locus_tag="KB942_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6852718.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5133..5594)
                     /locus_tag="KB942_002329"
     CDS             complement(5133..5594)
                     /locus_tag="KB942_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852719.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5612..6679)
                     /gene="ulaG"
                     /locus_tag="KB942_002330"
     CDS             complement(5612..6679)
                     /gene="ulaG"
                     /locus_tag="KB942_002330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBB6852720.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6897..7667
                     /locus_tag="KB942_002331"
     CDS             6897..7667
                     /locus_tag="KB942_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6852721.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7940..8767)
                     /locus_tag="KB942_002332"
     CDS             complement(7940..8767)
                     /locus_tag="KB942_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852722.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2657 a         1853 c         1383 g         2894 t
ORIGIN      
        1 taaaacatgg attatactgt tgaatttgac agtgaactac ttaagcaatt tccacaagaa
       61 gaacatttca tgtataaaga agcaatttcc cacaaagtga cagagtattt ggagcataat
      121 ttttttcgta taaagccagt gcgtactcgt tcggcaaaag gaatatacga gatgaaagtc
      181 aaaattaata aaaaaaatta tcgcatagca tttgctttaa aaaaaaatca tgtgttcatt
      241 ttttatatta gttcgaattt gcaaaaaatt gtatttgata aagaggtgag taaaaaaata
      301 aaatagctga gcattggagg atagattgag tgatactaag tctatcctct tgtttttcta
      361 aagaactaag ctagaatttt ttcttttgta cgttattata tgttatttgt cattaccaaa
      421 aagcaccttt tttcttctag tatctatgac ataagaaata ccacccattt cctttacttg
      481 aacaaatcct actgattcat aaaatttttt tgttttctca gtatcatctg tcaataattc
      541 gatttggact ggtccataac tagtgatctt ctccaaagct tcattcaaaa gcactttgcc
      601 tattccttgg cgctgatacc tcggatgaat gaggatatct tgaataaatg cgatcgtcgc
      661 aaaatcagtg atccaacgaa cgattccgat aatcttccct tccccattac gctttatcag
      721 agattcagat tgttcaaatg ctctagctaa ccttgcagtg tcttttgtgt aattcgtcca
      781 accaacgctc ttatataatt cagaaatctc atcgatcata agggaatcaa tattcatttt
      841 tttcttcctt tcttatacaa ttctccctct aataatacag ttctttgact atttaatcta
      901 tcacatataa ctctcctgta aagattctct cgattcctta aaattcctct ttccttttag
      961 aatgcatact ttttgataaa taaaaaggtt gtgataaatt ttcttcacaa cctctgtttc
     1021 tctctatcaa gtgttatctt ttaattttct acccttaccc tagcatctct attgcccgta
     1081 gtatgcatac tttccatgtt tgcgaaaata atgtttatct agtaaatatt gcgggatttc
     1141 acatatattg gggttgatca gctcgttttc tttggccatc agacaaattt catctaagat
     1201 caaactgttt tctactgctt tcataggtga atccccccat gtgaacggtc catgtccata
     1261 gacaagtact ccagggactt cgtttggatc taattttctt tccttaaacg tctcgacaat
     1321 cacattccct gtatgaactt cgtaagcttc tcttacttct tctttcgtta attggcgtgt
     1381 acaaggaacc ttaccataaa aagcatcagc atgagtcgtg ccataagctg gcaaatcgcg
     1441 tcctgcttgc gcccacatga cggagtgcgt ggaatgagta tgagtaatag cagttatatc
     1501 ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct gaagatggtt ttaagctatc
     1561 ttcttctatt atatttcccg acaaatctgt taccaccatt tgatctgctt gcatgcattc
     1621 atatctgaca ccgcttggct taatgacgat gactcctagc tcacggtcca cttcactcac
     1681 attgccccaa gtcaatttga ctaatccgta tcgcggcaat gccagatttg cttcaaggac
     1741 acgttgcttc attgctgcaa ttttttcttc acaagacatc atagccagcc tccttcaatt
     1801 tagaacttaa gtagtctttt gcctgctgga tttctcttcg aaagtcttgg cttttttcac
     1861 tccacatttc aatcagaaat gttccatcat aatctagacg tttcaaatgt cttaataatc
     1921 caagaaaatc aacacaaccg ctaccaaaag gaacatcacg aaactggcct gaatattctt
     1981 ttgttacagg gtaagtatct tttaagtgga tagcagtgat ataatcgatc cctttctcaa
     2041 gttctcgagc cggattattt tccggccatg cggacaaatt gcctaggtct ggatatactt
     2101 gtagaaaagg tgaatgaatc tgttctttga tttctaaaaa ttttgaaatg gagttcataa
     2161 atggatcatc catgatttcc acggaaagaa tgatgccata tttagaagct tcctttacgc
     2221 tttcttttat tccttcgata aagtcttctc tggaattcat tgatttctct tcataataaa
     2281 catcgtatcc agctatttgg attactttga cgcttagatt atgcgccaaa tggattgctt
     2341 ttttcattag ttgtttcgct gcttttttct tttctggatc agcagaacca aacggaaatc
     2401 gccgatgcgc acttaaacaa atcgaatgga tacgtacacc ggagctgaac atctcttctc
     2461 tcagctcagc tatcttttct tcagaccaat ctaatcgtgc taaccgctca tccgtctcat
     2521 caatcgacat ctcgataaaa tcgaagtcca tttcttttac aagggaaaaa cgttctttcc
     2581 atgaaatatc tttgggtaac gctttttcat aaatcccaat ttgagccatt tccttacccc
     2641 cagatacgaa tgatctcatc tttgaagttt tgtgcggcct tcatcggatc atttgctgct
     2701 gtaataccac gaccagtgat aaaggtatac acatcgatcc cttcaaataa gtgtaatgtg
     2761 tgtggattta atccaccagt tacagaaaca ttaaagccta gttcaatcag ttttttgatc
     2821 ttcgataaat ctttttcacc ccaagtttca ccagataaca gcgcatcacg gctttgatgg
     2881 tagattacct gccggatacc gatgttatgc caatccatgg cttgttcaaa agtccaattg
     2941 ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg ctgcctccat agttggaatc
     3001 gttgcacaac aaatgactgt catccagtct gctcctgctt ttgcaacatt actcgccact
     3061 gttcctccag cgtctgcaca tttcgtatca gcgactagtt ttttatctgg gaacatactg
     3121 cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa gaattgtgcc cacttcaata
     3181 atatctacta catcaccaac tttcataaca tctcctaatg catgttctaa gtcattgtga
     3241 tctaacgcaa tttgcaaatt cggcctgctc atcttagttc cttcctttac ttgttttttt
     3301 ggtaccactt tgtttcagca aatttttcac tgatttcttt gactgacatc acatttttga
     3361 taccgataac tgtaactcct tttttctctg cctgagtaaa catattcaaa aaattgactg
     3421 gcgtaaaaac tacatcgtat tggccagctg aactttttcc ctctgaaatg gcacaatgat
     3481 ggattttggt aattggtacg tttagttctt tcattgcttt ttccacactt ctcatcatca
     3541 tcaagcttgt tcctgatcca tttgcacacg atactaaaat tctcatctta atccctccat
     3601 tatgagtgtt gtctaatcag attttctttt atactctctg cttttctgca tatgtgtcat
     3661 agtcttctac aatcaaaaaa taaccatcag gatttttccg atattgtaac tgcggaatag
     3721 ccaataaacc aatgacaact acggcaattc cagcataacc taggactttc atgattactg
     3781 taaacaatgg ccaagctgtt gcccagtcga acatcccgat ataaccaccg tatgttgaca
     3841 tcccaatcca tgtagcgaat aatgctgaac cgcctacttg tatgattcct gaaagaaacg
     3901 ggaacaggca agcggctttg aatccacctc ggttattggc ataaactgct ataacggcat
     3961 tatcaaagaa caacggaatg aaaccggcta tgacgatcgt tggtgatttc aataaaatca
     4021 gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa gaacccaatc gttaccgcat
     4081 ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc tggaacagct cctggtaaaa
     4141 tcgtgtttga gattccttgg aaagattgag ttagttcatc gacgaatgtc cgaacaccta
     4201 attgcaagat cgccagatat accgcaaaat ttaaagaagt cgtcataatg tagaataaga
     4261 aactctgccc ttcttgcatg aattgcgctt gaatcaaata atctttgcct agtacaatca
     4321 gaatgatccc aaagaaaaat agcatgagta acgaagtagc aaccatattc tcattgaaaa
     4381 tcgacaagaa tcctggtaat ttgatatctt caattttttt gtcttgcttg attgatctat
     4441 ttttcttttc atcccgcttt ttcatccact cagctaattt tgcaaatata aagattccga
     4501 acatttgctg atgtgccact gcaaatccgg caccttctgt caattcctgc gtgattccta
     4561 ctgttaagtt agaagctact gtccaataac aacctaaaat cagtcccata aaaatcaatg
     4621 cttgtatcct acctagatca ggaaagcaaa acagtaaaat ccaaaaagcg gtagctgctt
     4681 gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag tttcgtatat ttctgaaaac
     4741 ggactaataa gatatttaaa acaaatgcaa tcagtaataa aatcatcaca tctccaaagg
     4801 tacggccaaa tgtctccatt aatccagcat ccacagcatt ttgtccaaaa taaggatctg
     4861 taaccatggc atccaaattg aaccgttcct ttagtccaac caagattggc cgaaaattat
     4921 tgaccaaacc accagagcca acagtcaaaa taaagtaacc gaccgttgcc tttaagaatc
     4981 cagcgatgca ttcatgaagc gggcgtttca gcaaaatata gcctaataac acgataaaac
     5041 caatcaggta tgctggctgc gtcaagatat ttgttgcaaa atacgtccaa atgcttaaca
     5101 aaaattcccc catctttttt ctcctctatc aattaattat attctgccat cacaacttgg
     5161 taatcatcga tggagttcgt agagatgaga cgctcaacca atccatctgt cattaataat
     5221 tcggataatt tttggatatt ttctagatgt tcttctggat cttttgctgc gagtgtaaaa
     5281 aacaaactag ccgttttctc ctcattctcc ccttcacttt caaaacggat tggctgtttc
     5341 aacttggtaa atgaaatagc tgttccaaaa acaccatcac ttttttctga agaatgcggc
     5401 attgctactt gaggtgcgat cacaatataa ggaccgtatt tttctacaca atggatgatc
     5461 tcctccacat acgtttcatc aataatttcc ttatcgatca atacaagaca gctttcggcg
     5521 atagcttcac gccaatccgt gatttcttta tctgggtaat gaatcaattg atggtcaaaa
     5581 aagtatttca gcaagaaact ccccttcttt cttataaaaa cgacggaaac ggtgtatcat
     5641 tttcaatcga gaagacatct tcaaatcctc gataaaaatt aaactcaaga tcatctttgt
     5701 ttgtcggata gatgaatttc ccgcctactt gccagatata aggtttaaat ccatacttca
     5761 ggcgatcctt tttgaatttc caaatttcag tgatctcttt tggatcagcc ataaaattag
     5821 accaaatatc gtagtggaca ggaatcacta cttttgcatt caatgactcc gccatgcgca
     5881 gcatatcaac agaagtaact ttgtctgtta tccctcgtgg gttttcacca taggccccta
     5941 aacagacatc gattttgtgt tcattaccat gtttggcaaa taaattggag taatgtgaat
     6001 ctcctgcatg ataaagattc cctcctgatg tttcgaataa atagtttact gcaatttgat
     6061 ccatctcctg aggcattttt ccttttaata cctcttctgg atcttcacaa gtgaccaacg
     6121 ctgtccgatc aaacgcttct aaagctacta cattgatgtc tttgattttg actgcatcac
     6181 ctggctttac tgtaatcgtt ttttcttcag gaattcccca tttttgccac gtgcgaacca
     6241 cttcttttgg cccaatgaat gttgcttcag gacaattttg atgaactgct gcggctgtat
     6301 ggatatctag atgatcggaa tggatatgtg taacgattaa agcgtctaca ttttttatcg
     6361 caaaaggatc gattacgaaa ggctgagtac gtaaattagg ctgcatattt tgaacgccac
     6421 tcatacgcat catctgatgg ccttttttca tcatgccatt cccatgacta cgttttcccg
     6481 tcccacacca tagatcacat aaaatattcg ttccttcatg acttttcagc caaattcccg
     6541 tacagcccag ccaccacata gcaaaagttc caggatccac tacttctgtt tcgatttctt
     6601 cattcaagta tgtgccccac tcaggaaatg tttctactaa ccacttttct tttgttactt
     6661 cattgacagt tttactcatt tgcttgctcc ccccatatcg ttgttccaac ttacaagtaa
     6721 atattaatgt gtatcagcat ccatgaaaac ccttttatat gaccgtttgt tttttatatg
     6781 attttctttc agtaatcaat gattgtttca gtataataaa gacagatatg aatgtttaaa
     6841 tgaacaacta gtcaaataaa tccatggatc aagatgtccc tcaggaggca aaagtcatga
     6901 aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat tttggaactg cttcagcaga
     6961 ctgaacaact atcaactttc gaattagctg aatcccttga tgtatctatt agtacgatac
     7021 gaagagattt aaatttatta gaagaaaaaa acgagatcat acgcaattac ggttattgtt
     7081 cttataatta caaaaaccag actgattttg atcaatctgg tcctgaaagg attaaacaag
     7141 cgattgccag agaagctagt aaatacatta acgattatga tacgctgttt atcaattcta
     7201 gctcgactgc gttgaaaaca ctggattttc ttcaatctaa ccatttgaca gtcattacaa
     7261 acaacgtgaa aatcaattac tctccgcaca aagataattg tagttatatt ttaactggtg
     7321 gagagatgcg ctttccaaaa gaagcactag tgggcgacat cgcattaaat acgatttctt
     7381 cagtcaatgc agacatttgt atcattggct gtagcggagt cgatgtagta catggggtca
     7441 caacaaagat attaaatgaa tcaacgatca acgaaatgat ggtcaacaag acaatcaaat
     7501 gtaagatttt agtcgctgat catcgaaaaa tcggactgac atcaaagttc aagattgctg
     7561 atatttcagt ctttgattat ttgatcactg ataaattttg ttctgaaaaa gtattagagg
     7621 agatcagaaa tacggggata aaaaccattc aagtagattc attttaagaa ccaggacgta
     7681 aatatgtaaa taggattatc aaaaaaatga ataacaaatt tatcgataaa tgaaaaacac
     7741 gctacgctca aattgataaa ggaatcccaa gatgtatcca tccaatcgat accatcataa
     7801 tctattgctt cttttataac cgttatatgt tgcctaaata ggaatcaaac aatatcgaaa
     7861 aaaggaggta tgacaaaagt cacacctcct tttttcgaat aaacggtatt caaatcagaa
     7921 cactgatttc acaaccactt tatttcaggt cgttttcaat cacctttacc gcgtaatcat
     7981 ttccgttgaa acgcgctatt ttttttgcgt ccatatatct ttctaaataa tcatcttctc
     8041 ctaattgctt atggcaagaa gcaatttcat aaagcaagta ttccaatgca taagtcgatt
     8101 tatgtttctt attatactca atacccttct cacataattg gattgcttta tcataatcct
     8161 cgattttacg gtaataggtg gcgatatcat aatatacaat gcagaccatc agattatcaa
     8221 aatcagtctc actcaatatt ttaagtgtac gctccacata aactttcgca aaatctaatt
     8281 ctccttttga caagtagatc attgccacgt ttgcagctgc ttgtaagtga tggatatctg
     8341 tttcggtcgt ttgttccaac acttgattaa aataaaataa tgctgcatct ggttttttcg
     8401 cataaagatg atcaagcagt ccttgataaa ataaaatttg atttaaaaga tcagaattac
     8461 ttactttttc ctgatcgatc gtttgtaaaa ttttataaga ttcttggtat tttgatacag
     8521 aattcaatga ttgaatctca tttatttttt tcctgatttt gtcttcttct gtttctacta
     8581 atatatcgga gatctcgaca ttcagctttt tgcaaagcgc acttaagata ttgatgcctg
     8641 gcgacatatt cttgttttcg atattgctga cagttgcttg ttcacaaatg ccttctgcta
     8701 attgtacttg tgttaacccc tttttctttc ggtattcttt tagtaatgct ccatctactt
     8761 taatcaaaac aatttccttc cctttaa
//
LOCUS       DADVXN010000106         8665 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.109, whole genome shotgun sequence.
ACCESSION   DADVXN010000106 DADVXN010000000
VERSION     DADVXN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.109"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(94..1137)
                     /locus_tag="KB942_002333"
     CDS             complement(94..1137)
                     /locus_tag="KB942_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBB6852723.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="KB942_002334"
     CDS             complement(1514..2479)
                     /locus_tag="KB942_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBB6852724.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="KB942_002335"
     CDS             2713..3558
                     /locus_tag="KB942_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6852725.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="KB942_002336"
     CDS             3749..4936
                     /locus_tag="KB942_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6852726.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="KB942_002337"
     CDS             4942..5298
                     /locus_tag="KB942_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBB6852727.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="KB942_002338"
     CDS             5300..5638
                     /locus_tag="KB942_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBB6852728.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="KB942_002339"
     CDS             6033..7067
                     /locus_tag="KB942_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6852729.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="KB942_002340"
     CDS             7060..7746
                     /locus_tag="KB942_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852730.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="KB942_002341"
     CDS             7770..8618
                     /locus_tag="KB942_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6852731.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2883 a         1574 c         1852 g         2356 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttc
//
LOCUS       DADVXN010000107         8578 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.110, whole genome shotgun sequence.
ACCESSION   DADVXN010000107 DADVXN010000000
VERSION     DADVXN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.110"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(176..2557)
                     /locus_tag="KB942_002342"
     CDS             complement(176..2557)
                     /locus_tag="KB942_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6852732.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2547..3194)
                     /gene="recU"
                     /locus_tag="KB942_002343"
     CDS             complement(2547..3194)
                     /gene="recU"
                     /locus_tag="KB942_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBB6852733.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3258..3797
                     /locus_tag="KB942_002344"
     CDS             3258..3797
                     /locus_tag="KB942_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBB6852734.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3887..4306
                     /gene="gpsB"
                     /locus_tag="KB942_002345"
     CDS             3887..4306
                     /gene="gpsB"
                     /locus_tag="KB942_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBB6852735.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4328..4695
                     /gene="rnpB"
                     /locus_tag="KB942_002346"
     ncRNA           4328..4695
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KB942_002346"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4904..6070
                     /locus_tag="KB942_002347"
     CDS             4904..6070
                     /locus_tag="KB942_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBB6852736.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6116..7612
                     /locus_tag="KB942_002348"
     CDS             6116..7612
                     /locus_tag="KB942_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBB6852737.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7693..8376
                     /locus_tag="KB942_002349"
     CDS             7693..8376
                     /locus_tag="KB942_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6852738.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2688 a         1565 c         1824 g         2501 t
ORIGIN      
        1 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
       61 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
      121 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
      181 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
      241 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
      301 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
      361 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
      421 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
      481 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
      541 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
      601 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggcgtca
      661 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
      721 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
      781 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
      841 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
      901 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
      961 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     1021 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     1081 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     1141 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     1201 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     1261 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     1321 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     1381 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     1441 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     1501 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     1561 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     1621 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     1681 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     1741 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     1801 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     1861 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
     1921 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
     1981 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
     2041 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
     2101 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
     2161 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
     2221 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
     2281 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
     2341 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
     2401 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
     2461 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
     2521 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
     2581 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
     2641 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
     2701 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
     2761 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
     2821 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
     2881 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
     2941 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
     3001 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
     3061 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
     3121 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
     3181 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
     3241 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
     3301 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
     3361 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
     3421 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
     3481 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
     3541 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
     3601 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
     3661 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
     3721 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
     3781 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
     3841 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
     3901 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
     3961 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaaactt acagcaaaga
     4021 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
     4081 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
     4141 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
     4201 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
     4261 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
     4321 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
     4381 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
     4441 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
     4501 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
     4561 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
     4621 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
     4681 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
     4741 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
     4801 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
     4861 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
     4921 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
     4981 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
     5041 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
     5101 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
     5161 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
     5221 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
     5281 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
     5341 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
     5401 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
     5461 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
     5521 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
     5581 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
     5641 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
     5701 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
     5761 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
     5821 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
     5881 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
     5941 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
     6001 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
     6061 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
     6121 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
     6181 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
     6241 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
     6301 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
     6361 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
     6421 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
     6481 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
     6541 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
     6601 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
     6661 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
     6721 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
     6781 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
     6841 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
     6901 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
     6961 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
     7021 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
     7081 aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata caaaatcaag
     7141 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
     7201 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
     7261 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
     7321 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
     7381 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
     7441 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
     7501 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
     7561 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
     7621 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
     7681 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
     7741 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
     7801 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
     7861 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
     7921 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
     7981 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
     8041 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
     8101 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
     8161 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
     8221 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
     8281 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
     8341 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
     8401 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
     8461 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
     8521 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttatt
//
LOCUS       DADVXN010000108         8494 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.111, whole genome shotgun sequence.
ACCESSION   DADVXN010000108 DADVXN010000000
VERSION     DADVXN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.111"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..173)
                     /locus_tag="KB942_002350"
     CDS             complement(<1..173)
                     /locus_tag="KB942_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6852739.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="KB942_002351"
     CDS             complement(593..1714)
                     /locus_tag="KB942_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBB6852740.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="KB942_002352"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="KB942_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBB6852741.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="KB942_002353"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="KB942_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBB6852742.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="KB942_002354"
     CDS             complement(4304..5215)
                     /locus_tag="KB942_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6852743.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="KB942_002355"
     CDS             complement(5232..6239)
                     /locus_tag="KB942_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6852744.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="KB942_002356"
     CDS             complement(6236..8365)
                     /locus_tag="KB942_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6852745.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="KB942_002357"
     CDS             complement(8370..>8494)
                     /locus_tag="KB942_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6852746.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DADVXN010000109         8022 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.112, whole genome shotgun sequence.
ACCESSION   DADVXN010000109 DADVXN010000000
VERSION     DADVXN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.112"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            104..805
                     /locus_tag="KB942_002358"
     CDS             104..805
                     /locus_tag="KB942_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6852747.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1414..2430)
                     /gene="tsaD"
                     /locus_tag="KB942_002359"
     CDS             complement(1414..2430)
                     /gene="tsaD"
                     /locus_tag="KB942_002359"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBB6852748.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2427..2891)
                     /gene="rimI"
                     /locus_tag="KB942_002360"
     CDS             complement(2427..2891)
                     /gene="rimI"
                     /locus_tag="KB942_002360"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6852749.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2898..3440)
                     /gene="rimI"
                     /locus_tag="KB942_002361"
     CDS             complement(2898..3440)
                     /gene="rimI"
                     /locus_tag="KB942_002361"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6852750.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3424..4146)
                     /gene="tsaB"
                     /locus_tag="KB942_002362"
     CDS             complement(3424..4146)
                     /gene="tsaB"
                     /locus_tag="KB942_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBB6852751.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4337..5317)
                     /locus_tag="KB942_002363"
     CDS             complement(4337..5317)
                     /locus_tag="KB942_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6852752.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5341..6825)
                     /gene="galT"
                     /locus_tag="KB942_002364"
     CDS             complement(5341..6825)
                     /gene="galT"
                     /locus_tag="KB942_002364"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBB6852753.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6837..7826)
                     /gene="galE"
                     /locus_tag="KB942_002365"
     CDS             complement(6837..7826)
                     /gene="galE"
                     /locus_tag="KB942_002365"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBB6852754.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2267 a         1712 c         1414 g         2629 t
ORIGIN      
        1 taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa
       61 aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat
      121 cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga
      181 gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt
      241 cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga
      301 acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt
      361 tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga
      421 tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga
      481 acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat
      541 ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa
      601 agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt
      661 aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca
      721 actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct gtaagacagc
      781 tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc tgataaatat
      841 cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt tttagatcac
      901 tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca ctgtttgcaa
      961 aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta gattattcga
     1021 tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga taatgataaa
     1081 taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa aatagggctt
     1141 tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc aactcttcgg
     1201 tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt ccttgtgaat
     1261 cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag cttgcatacg
     1321 cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg ctgttcttat
     1381 tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca agcttggatc
     1441 agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg ctgctgctcc
     1501 aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt ctacctcagg
     1561 gagtttagct gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc
     1621 tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg ttttagtaat
     1681 caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt ctagatcttc
     1741 tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc cgctaaaact
     1801 gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat cttttccctg
     1861 atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata ctcttccaac
     1921 tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc
     1981 atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg ccatcaatgg
     2041 aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat ggtttacagg
     2101 aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac caatcagcaa
     2161 cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt ctgccgacac
     2221 accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt gatggcggct
     2281 agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag aagcaacgat
     2341 gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat cacaacttga
     2401 ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa ctttctgctc
     2461 ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc taatatacga
     2521 aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac ttctaaaaaa
     2581 acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc
     2641 ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc atctattact
     2701 ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag ataatcagag
     2761 tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc atgtggataa
     2821 gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa atcttttttt
     2881 ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa cgcatcttct
     2941 tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa tttccgataa
     3001 agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc acagcgattc
     3061 attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt acgctgatat
     3121 gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct tgaaccgata
     3181 aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag cggtgacttc
     3241 aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac ttctcgttcg
     3301 acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta acgacgatcg
     3361 atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct tctggcatac
     3421 gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca accatttttc
     3481 ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg caactggttc
     3541 aactaaagca cctaattgag ccacagttac accacttgga atatcccatg ctggaactgt
     3601 attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt gacaatcact
     3661 cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat cagaagcaga
     3721 aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt atgccccagc
     3781 gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca caccgatgca
     3841 attagccgcg atggttttca gactagatac tcctactagc tctttatcta atgtataagc
     3901 aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg gaccatcaga
     3961 aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca attggtcgat
     4021 tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt gtccaagaat
     4081 ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta ttcctaatgt
     4141 aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag catacttttt
     4201 tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga ttatctgctg
     4261 gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact ccattttccc
     4321 acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa
     4381 tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct
     4441 ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca tatttcgcta
     4501 cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa cctttttctt
     4561 gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa
     4621 catgcggatt cgtttttaaa tattgcttca ctgcttgata tcctgactcg acagaaaaat
     4681 ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca
     4741 taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca
     4801 tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga acacttgact
     4861 taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca
     4921 aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga
     4981 tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt
     5041 ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa agatcttcaa
     5101 gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat
     5161 ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg
     5221 ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa
     5281 ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt
     5341 ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac
     5401 tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt
     5461 gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg
     5521 atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg
     5581 tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt
     5641 tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac
     5701 attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg
     5761 cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc
     5821 cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca agtttttctc
     5881 tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg
     5941 ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg
     6001 tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta agtcagcatt
     6061 cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc
     6121 ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata
     6181 agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg
     6241 gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag
     6301 catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc
     6361 tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg
     6421 aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt
     6481 aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt
     6541 gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc
     6601 tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa
     6661 gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac
     6721 catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc
     6781 gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat
     6841 tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat aatcgctttg
     6901 atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact tgaagcgact
     6961 aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc
     7021 acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg
     7081 aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc
     7141 aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct
     7201 ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga
     7261 tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg
     7321 aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt
     7381 tccatcatca atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg
     7441 attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc
     7501 acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa
     7561 tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact
     7621 ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct
     7681 tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc
     7741 acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg
     7801 gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt
     7861 gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa
     7921 aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat tttcaagggt
     7981 tttttgcaaa ggtttacaaa caaacgtaaa actagccttt tt
//
LOCUS       DADVXN010000110         7811 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.114, whole genome shotgun sequence.
ACCESSION   DADVXN010000110 DADVXN010000000
VERSION     DADVXN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.114"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="KB942_002366"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="KB942_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6852755.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="KB942_002367"
     CDS             complement(1383..1901)
                     /locus_tag="KB942_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852756.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="KB942_002368"
     CDS             complement(1917..2243)
                     /locus_tag="KB942_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBB6852757.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="KB942_002369"
     CDS             complement(2256..4235)
                     /locus_tag="KB942_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6852758.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="KB942_002370"
     CDS             complement(4256..4570)
                     /locus_tag="KB942_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBB6852759.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="KB942_002371"
     CDS             4738..5031
                     /locus_tag="KB942_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB6852760.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="KB942_002372"
     CDS             complement(5109..6221)
                     /locus_tag="KB942_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6852761.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6608..6787
                     /locus_tag="KB942_002373"
     CDS             6608..6787
                     /locus_tag="KB942_002373"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852762.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6981..7151
                     /locus_tag="KB942_002374"
     CDS             6981..7151
                     /locus_tag="KB942_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6852763.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7575..>7811)
                     /locus_tag="KB942_002375"
     CDS             complement(7575..>7811)
                     /locus_tag="KB942_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6852764.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DADVXN010000111         7736 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.115, whole genome shotgun sequence.
ACCESSION   DADVXN010000111 DADVXN010000000
VERSION     DADVXN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.115"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..108
                     /locus_tag="KB942_002376"
     CDS             <1..108
                     /locus_tag="KB942_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HBB6852765.1"
                     /translation="GNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(177..905)
                     /locus_tag="KB942_002377"
     CDS             complement(177..905)
                     /locus_tag="KB942_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6852766.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(918..2198)
                     /locus_tag="KB942_002378"
     CDS             complement(918..2198)
                     /locus_tag="KB942_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6852767.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2339..2941)
                     /locus_tag="KB942_002379"
     CDS             complement(2339..2941)
                     /locus_tag="KB942_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6852768.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3186..3392
                     /locus_tag="KB942_002380"
     CDS             3186..3392
                     /locus_tag="KB942_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852769.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3704..4423
                     /locus_tag="KB942_002381"
     CDS             3704..4423
                     /locus_tag="KB942_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6852770.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4504..5187)
                     /locus_tag="KB942_002382"
     CDS             complement(4504..5187)
                     /locus_tag="KB942_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBB6852771.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5406..7592
                     /locus_tag="KB942_002383"
     CDS             5406..7592
                     /locus_tag="KB942_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBB6852772.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2775 a         1301 c         1463 g         2197 t
ORIGIN      
        1 ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac attttatcca
       61 gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata ggcaaaaaag
      121 aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg ttctttttat
      181 gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt tgccaacgtg
      241 acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac ataggattta
      301 tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc atagaattgt
      361 aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga agtagaaaaa
      421 tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat tttgaagata
      481 tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat atgctcgaca
      541 ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc gatgttgctt
      601 tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt tcctaaagga
      661 tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc ttctagttcg
      721 atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt gagaagttct
      781 tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat gattctattt
      841 ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat ataaatggga
      901 atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa acgctggttt
      961 tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc caagataaac
     1021 aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg tttcaactgg
     1081 tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga atgattcttc
     1141 ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc aatcgcattg
     1201 tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc cagttcgatt
     1261 ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat gagttttaat
     1321 gacagtaaac tacggatctc aggaactttc atattatcca acatacttag attggccgga
     1381 aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga ccgtaatcct
     1441 tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg gtaatcatgt
     1501 ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc ctgttgcatc
     1561 ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt ccaataataa
     1621 accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt caaagctaag
     1681 acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta tgtaaataat
     1741 atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca agtctctaac
     1801 gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac aatgtgaaaa
     1861 tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa atcgccgatg
     1921 cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc gtaaaccaat
     1981 tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat agcaattaga
     2041 gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga aatcagaatg
     2101 actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat acaaattgtg
     2161 taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc ccattgattt
     2221 ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga ttttgaattc
     2281 agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag ttaattgttt
     2341 aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt gtaataggaa
     2401 gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag taagaagctg
     2461 agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc tttaataatg
     2521 ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca tagcgaagca
     2581 gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata gtcaattgca
     2641 gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc agacaaacga
     2701 attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa gaaagaaagg
     2761 tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg accgctagtt
     2821 ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac accatatctt
     2881 cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact ttatctttca
     2941 tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg tcttttgttt
     3001 cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc ttcgttttcc
     3061 aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta tgacaagttt
     3121 gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt gatggaggga
     3181 ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt aaatctgatt
     3241 tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt gtgatgatac
     3301 gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac tggcagttag
     3361 ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga gagcataaag
     3421 aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa aacagctttt
     3481 catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact gtttatttga
     3541 aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag gagttacttt
     3601 tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt ttatttctat
     3661 aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga taattgtgat
     3721 cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc ttgcccaaga
     3781 tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc cgctatttta
     3841 ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca aacgcttttt
     3901 ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca tctacgaaga
     3961 tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag aagtaaagca
     4021 gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc cacaaaagaa
     4081 accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga gaatcaaaaa
     4141 aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct actataaaga
     4201 actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca aaatagtgat
     4261 cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa tcgtacaaga
     4321 aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga tccgctacac
     4381 tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa actaagcaaa
     4441 ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt tgattcattg
     4501 agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg tctgagtgag
     4561 ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa acagatgatg
     4621 acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta gatagtggaa
     4681 aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt tcgttgctac
     4741 cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga ttttatcaaa
     4801 agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg cgggatgctc
     4861 atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata gaacaaacaa
     4921 gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg ttagaagaat
     4981 ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc attctccgtc
     5041 aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa aagcaccata
     5101 ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa gtcctattgt
     5161 acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat atttttgtct
     5221 tccttatctt agcataaacg attgaaaaaa aaggaaaaat tttcagaaaa ctaaaatcat
     5281 atcgcttccc attttcattt ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga
     5341 aactttaaat aaccaacttg aaataaacaa attcaaagaa gaaagatggg aaggaaatca
     5401 atcagttgaa tgaaaaagaa attctccgat tacaaaaaga attgaaacgc tgggagaaca
     5461 agtataaaaa gcttcaacaa gtctttcggc tacaagaaga aatcattgct acctatgaag
     5521 cggcttatgg cgaagcaccc ggacttattg aggaagaagt acaagtaaaa gaaagtgaag
     5581 gaaacagctc tttctttaaa atgatggaga aacgttccta taatcgcttc aatcaagaac
     5641 aaaaagcagc tatcacatat gatatgaaga aacatcttcg aatcatagca ggagctggga
     5701 gcggcaaaac gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag
     5761 acgaagaacg catactaatg atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac
     5821 gagtagataa tttcagccag agaaaaaccg ctgtccatat cggaacattc catagtatct
     5881 tttttaggat atacaatgaa atttgtcgaa aatttcctga agtagcaatg caaggaatac
     5941 aaggagactt ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa cagctgatac
     6001 gtaaatataa tctttatata tttgatcaat atggagaaaa aacgattgct tctcggttag
     6061 actattggca gaatatgaac ctttcagtag aagaaatggt tcagttagtt aaagaaaaat
     6121 atgattccat tgataagaat gcacgacagc cgatatcaga acgactgtat ggattgctca
     6181 cggaactcca agaacaaaaa agaagccagc aattgctaga gttcaatgat gtactccaaa
     6241 atctgaaaag cgctttggag aatgaggaga tccagcatta tatcggccaa aaatatgatt
     6301 atctattcat tgatgaattt caagatacta atccacttca atgggaaatc gtgaaacgga
     6361 tgacaaagga aaacaggatc aagttgattg ttgttggaga tgatgatcag agtatctatg
     6421 gattcagagg atcagagcca gcatacatca agaactttga aaaagaatat cctaccaaaa
     6481 ctcttttctt attaactaat tatcgttctc gggcagctat tgtccaagga gcaaatagat
     6541 tgatcagcta taataaaaat gatcggatac caaaggcgat gattccagca caaaaagaag
     6601 caggtgttat ggaagcctat ttgttttctg atactaaagc agaaagtcag tggctaatca
     6661 aacaaattca gacatttatt caaaaaaatg ggaaatataa ggaatcaatc atattatacc
     6721 gatcagcttc acaaacgcag cagcttgtcc aatctctgct gaaaacgaat attccttttg
     6781 tactggaagc agatagtcca tatgaaggta tctttggtat caagtctttc caacatttct
     6841 atcaaaaaat tgttcattgg caaacagcac tgaatctgca aaagaaaaat caggcctacc
     6901 agcaattgct ccggcagctg atggcagact gctacctgaa aaaaagtgaa tgcgatcaat
     6961 acttttctcc taaatatcaa aatatagccg ttgcagatta tattttgtcg ataagcccca
     7021 atttaaaatc gaaatctgca gaaatccgag agtttgagga agcgttgcgt aaaacagacc
     7081 agcatcaaga aatctattcg cttgttcaaa gctatcttca tctgccacgt atagctaagg
     7141 aaatcgacca gagtgatcaa gaatggatac gagaagaagt aaagcagcat gctactttca
     7201 gaagactcca agccttatcg caagaaaccc aagcgacagc taaagaatta aaaacccgct
     7261 tgattgctta cagacaagga aaacttgatg cactatgtat ccagagcatt cacaaaagta
     7321 aaggtcttag ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt ttgccttata
     7381 acggagcgac agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga
     7441 cgatagaaga agaacgaaga ctattttatg ttgcgatgac tagagcaaga aatcgtctgt
     7501 atctatgtgt acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag
     7561 aaaccgggag tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac agaagtggtc
     7621 agtttcaaga aagaagaagg aatgtacgaa aattgcttct caaatttttg tgaaatttca
     7681 gcttattttc gaagaagcta tttctggaac acagtttatt cggaaaaggg aggtgt
//
LOCUS       DADVXN010000112         7694 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.116, whole genome shotgun sequence.
ACCESSION   DADVXN010000112 DADVXN010000000
VERSION     DADVXN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.116"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            17..2344
                     /locus_tag="KB942_002384"
     CDS             17..2344
                     /locus_tag="KB942_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6852773.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="KB942_002385"
     CDS             2470..2799
                     /locus_tag="KB942_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6852774.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="KB942_002386"
     CDS             2839..3843
                     /locus_tag="KB942_002386"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBB6852775.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="KB942_002387"
     CDS             3840..4286
                     /locus_tag="KB942_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6852776.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="KB942_002388"
     CDS             4436..5251
                     /locus_tag="KB942_002388"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6852777.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="KB942_002389"
     CDS             5457..6629
                     /locus_tag="KB942_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6852778.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7324)
                     /locus_tag="KB942_002390"
     CDS             complement(6839..7324)
                     /locus_tag="KB942_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852779.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQEAI
                     AQQQAEQARQQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     S"
     gene            complement(7321..7515)
                     /locus_tag="KB942_002391"
     CDS             complement(7321..7515)
                     /locus_tag="KB942_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852780.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2576 a         1456 c         1536 g         2126 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt
     7081 ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt
     7141 cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag
     7201 caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag
     7261 ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg
     7321 tcactttttt tctagtgcag ctttttccgc tttttgtttc tcttcttttt tcttttcaat
     7381 ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7441 agcctgatct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7501 acttcctgcg accaaaaaca atgcaactaa ttttttcgct aaatttcctc cttaagtaaa
     7561 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7621 gttatgaata gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg
     7681 ctatagttct gtta
//
LOCUS       DADVXN010000113         7672 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.117, whole genome shotgun sequence.
ACCESSION   DADVXN010000113 DADVXN010000000
VERSION     DADVXN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.117"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            167..3394
                     /gene="fss3"
                     /locus_tag="KB942_002392"
     CDS             167..3394
                     /gene="fss3"
                     /locus_tag="KB942_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6852781.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3507..3821
                     /locus_tag="KB942_002393"
     CDS             3507..3821
                     /locus_tag="KB942_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6852782.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3834..4208
                     /locus_tag="KB942_002394"
     CDS             3834..4208
                     /locus_tag="KB942_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6852783.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4218..4553
                     /locus_tag="KB942_002395"
     CDS             4218..4553
                     /locus_tag="KB942_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBB6852784.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4636..5976
                     /locus_tag="KB942_002396"
     CDS             4636..5976
                     /locus_tag="KB942_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6852785.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6054..6728
                     /locus_tag="KB942_002397"
     CDS             6054..6728
                     /locus_tag="KB942_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6852786.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6721..6885
                     /locus_tag="KB942_002398"
     CDS             6721..6885
                     /locus_tag="KB942_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852787.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6961..7395
                     /gene="dcm"
                     /locus_tag="KB942_002399"
     CDS             6961..7395
                     /gene="dcm"
                     /locus_tag="KB942_002399"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6852788.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7396..>7672
                     /locus_tag="KB942_002400"
     CDS             7396..>7672
                     /locus_tag="KB942_002400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6852789.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLL"
BASE COUNT         2552 a         1295 c         1700 g         2125 t
ORIGIN      
        1 tctaatttaa aggcagtata tttcgtattt tagattaaat ttttaggctc attcattctc
       61 aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa agatgttcaa
      121 gcgagcatct tatttttttg tgcttaattt gaaaggagaa catttcatgt ataaaaaaca
      181 aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa ttgttggagc
      241 aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg taaccgtaga
      301 actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc
      361 agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata ttttctgtat
      421 tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa acccgttacc
      481 agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc
      541 agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca
      601 ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaataaa
      661 tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg ctaaaacagt
      721 attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga
      781 ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt tgataatcac
      841 cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg
      901 aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg cgattgataa
      961 acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa agatcaaaaa
     1021 agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag actttggaaa
     1081 aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat tggatgagat
     1141 tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt atacgattga
     1201 taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga cttcaaaaaa
     1261 cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag ggagtgatct
     1321 ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag acagtccaac
     1381 cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa caccaaaaga
     1441 gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag ctagtcatgg
     1501 gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc aaaccgtagc
     1561 ccctgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa ccactttgac
     1621 gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg agggtgctga
     1681 atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg cttttaaacc
     1741 agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt tagacgaaaa
     1801 gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag aaacaaaagc
     1861 gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga
     1921 tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag tgattcgctt
     1981 tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa ctggcttcaa
     2041 tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag gtgccgaaga
     2101 tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa
     2161 tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat
     2221 tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg tgaagagtga
     2281 gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg tgaccgttcc
     2341 ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa tgctctatga
     2401 tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg gaaacaaaga
     2461 attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc tacatgaaat
     2521 caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa aagctgccca
     2581 agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa
     2641 caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac aagttttaaa
     2701 taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg aagcaggcga
     2761 taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca gttgtacagt
     2821 ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc agacctttac
     2881 tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg tactggatgg
     2941 ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta caaacaaaga
     3001 aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca ccaaaactgt
     3061 gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta cttttgcgga
     3121 aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa
     3181 atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga aacaaccgga
     3241 aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga ccttccctca
     3301 aaccggggag aaaaattcca atgttttact gttcattggt tttactttga tttttgcgac
     3361 ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac aagtaaatcc
     3421 taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa
     3481 actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca gatatggctg
     3541 aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa ggatatggac
     3601 ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa cgggccgatg
     3661 atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa gaccaaccgc
     3721 taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat cgtgggttta
     3781 ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat
     3841 taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt ttttccgcat
     3901 tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag gtaaaagaac
     3961 gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt atcccagcta
     4021 atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat ccaatcgtgg
     4081 ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc attaaggcgg
     4141 atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa aatgaaagaa
     4201 agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat
     4261 atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct gatgaaaaaa
     4321 tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa gaagactctg
     4381 atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa gtaaatgaaa
     4441 acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg ccggatgcta
     4501 ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg tagcagtaaa
     4561 tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt tcgattggag
     4621 gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca gcacttggtt
     4681 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
     4741 tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat tgaaatcgtt
     4801 tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg agctatgata
     4861 ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg acaaaagcta
     4921 gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag tgaaggcttt
     4981 ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc caaaatctgt
     5041 tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg gaaataccaa
     5101 gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa
     5161 aaggaactca aagactccta tgtggagtat actttactgt atgatatgat cgccaatcga
     5221 attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa aaatcaagta
     5281 tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg agggaagacc
     5341 tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt gtttatcctc
     5401 gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt ttattcacaa
     5461 aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc tcgtagcaag
     5521 gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct tggacttcca
     5581 cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac gacgaaagaa
     5641 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc
     5701 tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg aatccgcgat
     5761 caattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc catgatgttt
     5821 ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta tgtggataca
     5881 ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta tgatttctta
     5941 gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac tgactttata
     6001 cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt aatatggaaa
     6061 gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt tcttatatta
     6121 tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg agtcctgggg
     6181 ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat gtgattattg
     6241 atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct agtctctttc
     6301 aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat catggctatt
     6361 cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc tcgcgggcgt
     6421 tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa atcgcttgga
     6481 aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag gcatttatta
     6541 acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat ccagatgtat
     6601 tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat tatgctacgg
     6661 ataatctaga attattttta ccactagggt atagtaggaa agatttggag gttcaattac
     6721 atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag cattgcggat
     6781 ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt agccgatcag
     6841 gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga caaattaata
     6901 atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat gaggtaatgt
     6961 atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat ggaacaggcg
     7021 agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag ttataaagca
     7081 attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc agatgagttt
     7141 attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca agctttttca
     7201 attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt tgaaatcgct
     7261 cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg aggattgctc
     7321 aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg attggggtat
     7381 gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg ggagcggata
     7441 ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat cattggaccg
     7501 tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc caatcgagga
     7561 atgggtggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg tgatgaagga
     7621 atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt ac
//
LOCUS       DADVXN010000114         7598 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.118, whole genome shotgun sequence.
ACCESSION   DADVXN010000114 DADVXN010000000
VERSION     DADVXN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.118"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(941..1354)
                     /locus_tag="KB942_002401"
     CDS             complement(941..1354)
                     /locus_tag="KB942_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBB6852790.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="KB942_002402"
     CDS             complement(1810..2295)
                     /locus_tag="KB942_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852791.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="KB942_002403"
     CDS             complement(2434..2649)
                     /locus_tag="KB942_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBB6852792.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="KB942_002404"
     CDS             2873..3673
                     /locus_tag="KB942_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6852793.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="KB942_002405"
     CDS             3692..5560
                     /locus_tag="KB942_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6852794.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="KB942_002406"
     CDS             5553..6044
                     /locus_tag="KB942_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBB6852795.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="KB942_002407"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="KB942_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBB6852796.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="KB942_002408"
     CDS             6604..>7598
                     /locus_tag="KB942_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBB6852797.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DADVXN010000115         7410 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.119, whole genome shotgun sequence.
ACCESSION   DADVXN010000115 DADVXN010000000
VERSION     DADVXN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.119"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(16..345)
                     /locus_tag="KB942_002409"
     CDS             complement(16..345)
                     /locus_tag="KB942_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6852798.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(335..607)
                     /locus_tag="KB942_002410"
     CDS             complement(335..607)
                     /locus_tag="KB942_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6852799.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1058..1861)
                     /locus_tag="KB942_002411"
     CDS             complement(1058..1861)
                     /locus_tag="KB942_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6852800.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1839..2963)
                     /locus_tag="KB942_002412"
     CDS             complement(1839..2963)
                     /locus_tag="KB942_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6852801.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2979..3794)
                     /locus_tag="KB942_002413"
     CDS             complement(2979..3794)
                     /locus_tag="KB942_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6852802.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3784..4656)
                     /locus_tag="KB942_002414"
     CDS             complement(3784..4656)
                     /locus_tag="KB942_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6852803.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4741..6030)
                     /locus_tag="KB942_002415"
     CDS             complement(4741..6030)
                     /locus_tag="KB942_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6852804.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6258..6902)
                     /locus_tag="KB942_002416"
                     /pseudo
     CDS             complement(6258..6902)
                     /locus_tag="KB942_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1590 c         1135 g         2407 t
ORIGIN      
        1 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
       61 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
      121 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
      181 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
      241 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
      301 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
      361 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
      421 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
      481 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
      541 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
      601 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
      661 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
      721 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
      781 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
      841 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
      901 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
      961 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     1021 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     1081 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     1141 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     1201 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     1261 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     1321 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     1381 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     1441 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     1501 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     1561 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     1621 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     1681 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     1741 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     1801 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     1861 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     1921 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     1981 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     2041 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     2101 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     2161 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     2221 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     2281 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     2341 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     2401 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     2461 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     2521 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     2581 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     2641 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     2701 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     2761 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     2821 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     2881 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     2941 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     3001 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     3061 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     3121 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     3181 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     3241 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     3301 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     3361 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     3421 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     3481 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     3541 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     3601 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     3661 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     3721 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     3781 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     3841 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     3901 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     3961 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     4021 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     4081 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     4141 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     4201 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     4261 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     4321 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     4381 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     4441 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     4501 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     4561 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     4621 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     4681 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     4741 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     4801 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     4861 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     4921 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     4981 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     5041 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     5101 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     5161 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     5221 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     5281 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     5341 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     5401 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     5461 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     5521 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     5581 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     5641 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     5701 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     5761 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     5821 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     5881 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     5941 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     6001 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     6061 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     6121 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     6181 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     6241 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     6301 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     6361 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     6421 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     6481 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     6541 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     6601 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     6661 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     6721 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     6781 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     6841 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     6901 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     6961 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     7021 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     7081 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     7141 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     7201 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     7261 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     7321 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     7381 agcccacaaa aaaagctgtc ctcacagaca
//
LOCUS       DADVXN010000116         7396 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.120, whole genome shotgun sequence.
ACCESSION   DADVXN010000116 DADVXN010000000
VERSION     DADVXN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.120"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="KB942_002417"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="KB942_002417"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBB6852805.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="KB942_002418"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="KB942_002418"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBB6852806.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="KB942_002419"
     CDS             complement(2298..2960)
                     /locus_tag="KB942_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852807.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="KB942_002420"
     CDS             3144..4790
                     /locus_tag="KB942_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6852808.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="KB942_002421"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="KB942_002421"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6852809.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7396)
                     /locus_tag="KB942_002422"
                     /pseudo
     CDS             complement(5971..7396)
                     /locus_tag="KB942_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2314 a         1361 c         1367 g         2354 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacata
//
LOCUS       DADVXN010000117         7364 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.121, whole genome shotgun sequence.
ACCESSION   DADVXN010000117 DADVXN010000000
VERSION     DADVXN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.121"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..753)
                     /locus_tag="KB942_002423"
     CDS             complement(<1..753)
                     /locus_tag="KB942_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852810.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLL"
     gene            complement(873..1520)
                     /locus_tag="KB942_002424"
     CDS             complement(873..1520)
                     /locus_tag="KB942_002424"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBB6852811.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1604..3031)
                     /gene="uxaC"
                     /locus_tag="KB942_002425"
     CDS             complement(1604..3031)
                     /gene="uxaC"
                     /locus_tag="KB942_002425"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6852812.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3105..4184)
                     /gene="uxuA"
                     /locus_tag="KB942_002426"
     CDS             complement(3105..4184)
                     /gene="uxuA"
                     /locus_tag="KB942_002426"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6852813.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4187..5818)
                     /locus_tag="KB942_002427"
     CDS             complement(4187..5818)
                     /locus_tag="KB942_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6852814.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6122..6793
                     /locus_tag="KB942_002428"
     CDS             6122..6793
                     /locus_tag="KB942_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6852815.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2373 a         1451 c          998 g         2542 t
ORIGIN      
        1 gagtaagaaa atagccgcac caattataga aagtaatgcc ctatttttaa ctaacccttt
       61 taaagatttt aacaatgaaa ctttgtattg agattggtca attcttactc gttcagtagt
      121 tagtttataa catattaaat aacaaataag tgcacatatc gaaaatattc ccgcaacaat
      181 tgtaaaagtt gatccaccac gaactatttg agcgcctgaa tcatctcttg tataaataaa
      241 taatggtgtt aatacaccaa tgaccaaact tgctaaagta gctccaacat ttcggaatgt
      301 cgatagcatt gttctttctt ctgtaataga agtaatagct gatgccattg atccataagg
      361 aatatttata gatgtgtaaa aaatactccc ccataatata tacgtaacat acatgtatac
      421 taccttaacg ctcatagaag ctgaaatcat agaagtttga tacattaaaa aacttgctaa
      481 agcgactgga ccactcattc ttaaaatcca cggtttaaac ttaccctgct tagttgcttt
      541 cgatctatca acaatacgcc ccatagttac atcagtaact gcatcgataa atcttgctac
      601 taaaaataat gttccaacgg cagctccgct tatccccaat acttttgtat aaaagatcat
      661 taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca aaatctccaa acatatatcc
      721 tattttgtct cttataccaa attttctttc catacaattc tctcccttct gttgttaagc
      781 gttttcattc tgatgaaagt acgtactata ctctgaagcg taaagagtca gtatttattt
      841 tttacatttc agagtattat ttgttttttc tcctaataat tctttatctc ataaaactta
      901 tctatatatt tactagctac tcgacttact tcttcaaaat cacctttttc aacagaagcc
      961 aataaattcc cacctacacc aactgcaaca actcctactt caaaccaatc ttctaagttc
     1021 tctatactta ctccgccagt aggcattata tttacgtgtg gtaatggagc ttgataggct
     1081 tttacaatgg atggaccaaa agcagaacct gggaatagtt taatgatatc tacgccagca
     1141 gtaagtgcct cctttatctc agtaattgtc atacaacctg gtaaataagg aatttgatat
     1201 aaattacaaa tctcagctgt ttgtcgatca aaagatggac taacgatata ttctgcccca
     1261 gccataattg cgatacgagc agtaaacgca tctaaaaccg taccggctcc gattattaca
     1321 tctttttgat cagcatattt tttagcaatt tctttgatta tttcttcagc attaggaact
     1381 gtaaaagtta gttcaatccc tttaattcca cccgcaataa ttgaatttat tgctttaatg
     1441 gcctcatcct tatttttccc acgtacaacc gcaatgactc cagccgcttc taacttactt
     1501 aaaatttcta cttttttcat atctatctcc ttcctttatg gatacatact ttgtctagct
     1561 gtactaactt acaaagaata aatttaagct tttatttcaa gtattatttt ctacttccga
     1621 tttcaaaaaa attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac
     1681 taagatacat ttcatcatct ggaattcggc ctgctttaac ccactcgctt agtaattgac
     1741 ataatactcg acgaaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca
     1801 acatacctgt aaaattcgac aacaaacttt gttgtgccat cgtagtcaac tgttgacgca
     1861 ttccttcagc tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac
     1921 ccccttggaa gctttgcatt gctgttgcta gtggaagcca atcagttgga tttaatgaat
     1981 ataaaattgt ttttggtaag cacccactct tttcagcggt atcgaacaat gctacaacat
     2041 tttgtgtcaa actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt
     2101 cctttaatat acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat
     2161 atcttgtata ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg
     2221 ataaatggaa atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt
     2281 catctgctat ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt
     2341 gatgaaaatg atccactcgt tggctcaatg cttgcttcaa cccattaaag tctttgattg
     2401 gtatagccgc tgtttgacta agttttaaaa tatagttcga aaagcctgat gatttgatac
     2461 tccagacctt atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa
     2521 gctcagtatg ataccacaag tcatctgtag gatcatccgt tgtacagatg gctttcacat
     2581 ccatcatctc caacaattct ttcgggcgat aggaatccgt ttgaattttt tcgttagcac
     2641 gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa atcaatatca aagtaacgtt
     2701 gtaattccaa atgagaccat tcaaaaatag gattacctgg cgccttctcc actgttttta
     2761 caaaagctaa aaatttttca taatcatcac ctggaccact gatcaactct tcttctatac
     2821 cattagctcg catcaatcgc catttataat gatctccagc tcctttatca tttaaccata
     2881 cctgagtcaa attcttgtat tgcttatttt ctagcaaata tgctgggtct aaatgacaat
     2941 gatagtcgat aattggttgg tgttttgcaa atttatgaaa aagtctttta gctatttcat
     3001 tctctaataa aaagttctta tctaaaaaca tatttaaaca ctcctttcct tccgttagtt
     3061 tttagaagag aaaatttttc ctctaaataa actcccttat ataattattt actcgccccc
     3121 ttttcaagcg cttcccaaat accatttaaa taaactgcac ctagagctcg gtcatataat
     3181 ccataaccag gtctaccggt ttctccccaa atcatccgac catgatctgg acgaatatag
     3241 ccattaaatc cattatcgta cattgttttt aatatcttat acatatctag agaaccatat
     3301 tctgatttat gcggcgcttc gtaaaaatcg agtccattgt cacttaaata tttgatatta
     3361 cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt cagatagaat ttcatagaca
     3421 tcattatctg gtcgttctgc aatacttcca gtacataatg taataccatt agaaggagaa
     3481 tccactacat tacacaacca atctaaatcg tcacgatttt taacaatacg aggtaatccg
     3541 aaaatggaat atggtggatc atctgggtga actgccattt taatcccaac ttcttcacat
     3601 gtcgggataa ttgctttgag aaaatatgca aagttctctc gtaatttctc ttcatcaact
     3661 tctttatagg cttctaataa ttgcttaatt tgtgataacc gatgaggttc ccaacccggc
     3721 aattcaaaat tattagtaga attttctact tctctaataa tttcctccgg atcagtaggg
     3781 atattttttg aaatataggc taacgtagaa gacccatcat ctaaacgata atccaaatcc
     3841 gattttaccc aatcaaatac tggcataaaa ttataacaga taacctcaat cccaaattct
     3901 gccaaattgc gaatcgtctg tttataattc tcaatgtatt gatctcgtgt tggtaaacca
     3961 attttgatat catcatgaac attaacactc tcaataactt tcaaggtaag tccattggct
     4021 tcgacttcat cttttaaaga tttaattcgt tctctcggcc aaacctctcc ggctggtata
     4081 tccattaatg ttccaacaat tcctttcatt gctgggatct gacgaatttc ttgtagtgag
     4141 attttttctt catttgcccc ataccatcta aaagtcattt ccatagttac tttcctccaa
     4201 cttctgttat atatttattg attgttttac gaactgcttg aggacctact agcatttcgc
     4261 aaaagatttt ttcagttttc tttcctaccc caacttgata taaatctaaa ccaaagattt
     4321 ctttattttc taaaatttcc tgtaccgcac aatgaatctt tgaaggatct tgttcaccta
     4381 atttcaatac tgataattta tcctgcaact ctaccaataa cggatcagga cttggcacaa
     4441 aattttcccc ttgatcatcg actgccagta aataacgtaa ccaagcagca atagcgatag
     4501 gaataaatgt tagattttct gcatttccgt ctttttcgac atacttttta attgtttcac
     4561 catagcgaat cgctactttt tgagaagtat ctgttgcaat tcgttgtggt gtatctggaa
     4621 tatagggatt aggcaaacgt ttattaatta cttcatcaat aaattgtttt ggatcaataa
     4681 tgcctggatt ttcaactact ggtaaacctt ctaaataacc tattcctttg attaaaccaa
     4741 ccaaatcatc atcctgcatc tctgctgaaa ttgattgata tcctagcaga caacctgtta
     4801 ctgccaaagc agtatgaagt ggattcaagc ttgtcgtaac cttcatgatg tctgctttat
     4861 caaccgtctc tctatctgtc ataatcacac cagccttttc caatttaggc cggccatttg
     4921 gaaaactatc ttcaataact aaataatgaa ctacctcagt attagtgaaa gcagcaatat
     4981 tggtatgttt ttttgtgtga atgatatcta aatcctcgag ccccatcatc aatagatctt
     5041 cctttacatt ctctgaagga tttggcgtaa ttcgatcaat catactccaa ggaaaagtca
     5101 ctttttcttt atcctttaaa taagcaataa atttttcaga aacatatcct tctttttgcc
     5161 aagcttctgc aatcgtaaaa atactatctt gaaaacgttg accattctgt gagaaattat
     5221 cagtagaaac catcgcaatc ggcaactctc cagcattaaa tctgctatat aacaaggatg
     5281 ttacaatact catcgtatgt tgaggattgc taggtccaga tttgatatca gctttgacta
     5341 ccgaaaagaa ttgcccttta gaatctttta aactgtaacc tttttcagta atagtaaacg
     5401 ttgtaaactg taatttagga tcttcaaaat atttaaccac tctttgatag tccgctatat
     5461 tcttcggatt gcaatataaa ctatcagccg ttactgccaa aagtcttttt ttgagttcac
     5521 catctccact cataacaatt tccaaaatgt cattgttata gtcatgataa gctttttcca
     5581 ctacttcttc atcaaatgtt tcacacacaa ctactcctgt ttctaattca ttttgatctg
     5641 ctaagcattg agcaatttct gcatgaaatc ctcgataaag atttcctcca ccaaaatgaa
     5701 gccatactgg atgttgctgg ccttcttgct taacttcttt gacatcaaaa cacggcagta
     5761 gaatctgcat ttcattgaat tcttcgatag aattttgcca atttttaatc gaaaccataa
     5821 ttaacctcct caaactgata tgttagtttt gatacatttt atattttaaa acccattgta
     5881 accgttgtca atataaaaag ggaataaact tgcttttttc taccatatca gaatgattgt
     5941 attttttatt cagaaaaata aaatactcct tctttttgga ctgattttat cgaaacttat
     6001 tttttctttt tttcatgtat tcttttattt ttataactag tgtattactt caaaatttga
     6061 tattatatgg catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg
     6121 tatgacaatt aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat
     6181 ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc
     6241 tattggtcgt acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga
     6301 tacaattcct caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc
     6361 tcgatttgtc agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa
     6421 taaccaaaaa aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca
     6481 aaaaaatgct cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat
     6541 tgcagagcga gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt
     6601 tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta
     6661 tcaactattt gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt
     6721 gcatcttcat ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt
     6781 caaaaatata taacttttaa ctctttttaa attaaatggt tgttctgatc agaaaaatat
     6841 cgcttgtttt cttattatgt aatatatttc ataaactatt ctgcataaca actattgttt
     6901 gtacttttta cattggataa ctaattatga ctgctgataa cttttgatat taaactagta
     6961 tctccaatac aaataaaaaa tagatatctt gttcatcaaa attagttcgt ggatacttac
     7021 cttcatttac atcattcagc ggtttcccat tttcttttat tctattattt cattttctaa
     7081 tagacttggc ttcatttcgc aatacttttt ttcaataggc atcaaattgg tgttgtttag
     7141 atatttagtt gtagttttgt gacatgataa gtatgctcct ttttctcttt tccactaata
     7201 caacgatcaa agggtgcttt tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt
     7261 gattctatat atcctgtttc aactacatct tatatttcac aatagaataa atagcctttt
     7321 gcatagctgc ttaacttata agaaattgca atttacttct tcaa
//
LOCUS       DADVXN010000118         7310 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.122, whole genome shotgun sequence.
ACCESSION   DADVXN010000118 DADVXN010000000
VERSION     DADVXN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.122"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="KB942_002429"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="KB942_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6852816.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="KB942_002430"
     CDS             complement(1804..2697)
                     /locus_tag="KB942_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6852817.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="KB942_002431"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="KB942_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBB6852818.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="KB942_002432"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="KB942_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBB6852819.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="KB942_002433"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="KB942_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBB6852820.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1941 a         1630 c         1278 g         2461 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat
//
LOCUS       DADVXN010000119         7040 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.123, whole genome shotgun sequence.
ACCESSION   DADVXN010000119 DADVXN010000000
VERSION     DADVXN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.123"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            108..497
                     /locus_tag="KB942_002434"
     CDS             108..497
                     /locus_tag="KB942_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6852821.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            484..2931
                     /locus_tag="KB942_002435"
     CDS             484..2931
                     /locus_tag="KB942_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6852822.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            2936..5059
                     /locus_tag="KB942_002436"
     CDS             2936..5059
                     /locus_tag="KB942_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6852823.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5056..6060
                     /locus_tag="KB942_002437"
     CDS             5056..6060
                     /locus_tag="KB942_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBB6852824.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6079..6990
                     /locus_tag="KB942_002438"
     CDS             6079..6990
                     /locus_tag="KB942_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6852825.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
BASE COUNT         2278 a         1188 c         1556 g         2018 t
ORIGIN      
        1 ggcaattatt tggtaacgag tcgaggtgtt tttgagtatc catattaaca aaattattga
       61 agggtagttg atttctaaac tatccttctt tttaaaggaa tgatttgatg aagaaaataa
      121 aaagctacac cagtatttgg gcagttgaaa aggtcattta tgcaattaat gactttcaac
      181 tgccttttcc tgtgactttt aaccagatgg cttggtttgt tttatccttg ctgtttgtca
      241 tcgtatttgc ccatgtgcct ccactatcta tgatagaggg ggccttttta aaatacttag
      301 gtataccggt ggcagttact tggttcatgt cgcaaaaaac gtttgacggt aagaaaccat
      361 tgggattttt acgatctttt attagctatc acttacgatt taaggtcact ttcgcaggaa
      421 agaaagtcaa agaacagaag aaacgatggg acgaacccat tactctggta aggagtgtga
      481 actatgtacc cgattaagta tattgaaaat aatcttgtct ttaatcaaga gggagaatgt
      541 tttgcctact atgagttaat accttacaat tactcctttt tgtcacctga acagaaatat
      601 caagtccacg ataactttcg tcaattgatt gcgcaaaatc gtgaaggcaa gattcatgcc
      661 ttacaaattg ccacagaaag tagtattcgt gccacgcaag aacgatcaaa aaaggaaatt
      721 accgggcgat taacagaagt agcgaaacaa cgaattgatt tacagacaga ggcattagta
      781 tccatgattg gcgatagtca gattgattat cgctttttta ttggcttcaa actgattgcg
      841 acagatgaag aggtcaatct aaagaatctc aagaaatctt tcttctccgg ctttcaagaa
      901 tttgtctatg gagtgaatca tcatttgatg ggcgattttg tttccttatc caatgaagaa
      961 attcgccgat ataccaagct tgaaaaacta atggaaagta aattggctcg aagatttaaa
     1021 gtcagacgag tcacaccgag tgatctgatg tatttgattg aacatatcta tggcgaaaag
     1081 ggtacaccat ttgaagaata tgaatttcag ctaccaaaga aaaagctgaa gtcggaaact
     1141 ttggtgaaac gttacgactt attacgtccc agtcgttgtt tgattgaaga aaagccccgg
     1201 tacttatgca tggaacatga gaatcatgaa tcatatgtag cgtacttaac aattaataca
     1261 attgtggggg aaatggagtt tccctcctca gaacttttct attaccagca acaacaattt
     1321 acattcccaa ttgacactag tatgaatgtg gagattgtga cgaacaaaaa ggcacttgct
     1381 accgttcgca ataagaaaaa ggaattaaaa gacttggaca accatgccta tcaatctgac
     1441 aatgaaacaa attccaatgt cttagatgca ttggattccg tagatgaatt ggaaactacc
     1501 ttggatcagt ccaaagaatc catgtacaaa ctgagctatg tggttcgagt aagtgccgaa
     1561 tcagtagatg aactaaagcg ccggtgtgat gaagtgctag atttttatga tgataccaat
     1621 gtaaaactag tccggccttt tggcgatatg atggggctac atgaagaatt cttaccttct
     1681 agtaagcgat acatgaatga ttatatccag tatgttacgt ctgattttct cgccggactt
     1741 ggttttggcg ccactcagat gttaggggag ttggaaggca tttactttgg ttataacgta
     1801 gatacggggc gcaatgttta cctgaaaccg gcattggcct cacaaggggt caaggattca
     1861 gttaccaatg cgctagcagc ggcattcctc ggttcactcg gtggcggaaa gtcctttagt
     1921 aacaatctct tagtttatta cgccgtcctt tttggtggac aagctgtgat tgtcgatcca
     1981 aaaggggaac ggggtggctg gaaagaaacc ttgccagaaa ttgcggatga aatcaatatt
     2041 ctcaacttaa ctagtaagcc aaaaaatcaa ggattacttg atccttatgt gattatggag
     2101 aaaaagaagg attctgaaag tctagcaatt gatattctga cctttttaac aggaatttct
     2161 agtcgagatg gggagaagtt tccagtgttg cgtaaggcca ttcggacggt tacccaaagc
     2221 aatcaacggg gattattaca agtaattaat gagcttcgaa aagaaggctc accagttgcg
     2281 gaaaatatcg cggatcacat cgagtccttt accgattatg attttgccca tttgcttttc
     2341 tcagatggtt cgattaccca gtcgattagt ttagataggc agttaaatat cattcaagta
     2401 gcggatttgg ttttaccaga tgcagaaacc acttttgaag aatataccac catggaattg
     2461 cttagtgttt ccatgctaat cgtcatttcc acttttgcct tggactttat ccatagtaat
     2521 cgagaaatct tcaaaattgt agacttagat gaggcgtgga gttttcttca agtagcgcaa
     2581 gggaaaacat tatcgaataa gctcgttcgt gctgggcggg ctatgaatgc cggggtttac
     2641 tttgtcacgc aaaatagtga tgatttactg gatgaacgct tgaagaataa tatcgggttg
     2701 aagtttgcgt ttcgcagtac cgatattcat gaaatcaaga aaaccttgga attctttggc
     2761 ttggatcgag aagatgaaag caatcaaaaa cgcctacgag atttggaaaa tggccaatgc
     2821 ttgatgcagg atttgtatgg tcgggtaggc gtggttcagg tacatccagt ctttgaagaa
     2881 ctcttccatg cctttgatac ccgtccacct gttcaagaga gaatggagtg aggggatgaa
     2941 aaagaaactt tttcggattg tgggaatcgt ttttattagc atactcactc tgatagttct
     3001 attgagttta gttggtacgg tagccgaggc gacagggcta gttgatgata cagttgaagc
     3061 aggaaatcta tattccaaat attcactgaa caactatcaa ctagatttct ttgtggatag
     3121 ctcttgggat tggttgcctt ggaattgggg tgatggctta gggaaaagcg tgatgtatgg
     3181 tctttatgcc attactaatt tcatttggac ggtgagtttg tatttgtcga acgccacggg
     3241 ttatgtggtt caagaggctt acaaattaga tttcatttcg gatacggctg aaagtattgg
     3301 aaagaatatc caaacattgg ctggtattac tgaaaatgga ctccagacct ccggatttta
     3361 ttttgggttt ctattattga tgattttagc tctgggggtg tatgtggcgt ataccggatt
     3421 attaaaacga gaaaccacga aagcagttcg agccgtgatt aattttgtga tgatttttct
     3481 actatcgggt tcgttcattg cttatgcgcc tacctatatc acaaaaatca atgactttag
     3541 ctcagatgtt agtgaagcgg cccttaccct tggaaccgag atcgttgtac ccaattcaga
     3601 aagccaagga aaagatagtg tggatttgat tcgggatagt ttgttttcga ttcaagtcca
     3661 acaaccttgg ttactactgc aatttgatga ttccaacata gaagaaattg gtgaagaccg
     3721 ggtcaacaag attctatctg tgagtccaga tgaaaacaaa ggcaaagatc gggaagaagc
     3781 ggtcaaggca gagattgaag acaatgacaa tgcgaatctc agtattacca agaccatgaa
     3841 ccgtttaggg accgtggtct ttttggtatt gttcaatatt ggcatttcct tctttgtttt
     3901 tcttctaaca gggattatgt tgttctcgca aattctcttc attatctttg cgatgtttct
     3961 gccaattagt ttcttattgt ccatgttgcc gacttatgaa agtttaggaa agaaggcgat
     4021 tattcgttta ttcaatacga ttatgatgcg agcgggtgtt actttggtga ttacgactgc
     4081 atttagcatc tccacgatgt ttttcaatat ttcggcaacc tatccatttt tcatggttgc
     4141 ttttctgcaa attgtgacct ttgccggtat ctatttcaaa ttgggcgaca ttatgagtat
     4201 gttcaacctc cagagtaatg atagccagtc aatgggaaga cgcgtcatgc gaaaaccgca
     4261 aatgttaatg aatcgaaaat tgcggcaact caatcgaaat gtgggacgag ctttggcttt
     4321 tggtggtggt gcagcagtcg gaaataagtt ggcaaaggaa caatcaaaat ccaaatttaa
     4381 gccatccggt tcttctctac gaaagaattc acgattaccg aacgatcacg aaatactatc
     4441 tgacttaact aaggagaatc cagtttcaaa taataagaaa caatcccgaa tgaatttggc
     4501 tgggagaaaa atcggcaaag ttttggatac ccaagctttg gtgaaagata agactaaaca
     4561 agtaaaggat caggtaagga atacgccaac gaatttgaaa tacaatctcc ataaaggaat
     4621 cgaaaagtcg aaaaaagcac cagaagaatt caagcgcgga ctgttccaag aaaaagcgga
     4681 tcgagtggat ttgcgagaaa aacaacgaca acgaagaaat gcgaaaatgg acgaaaaacg
     4741 caagatactg agtgaagcaa caaatcgtca gggaaaaaga agggcgaata ttccaataga
     4801 taaagcagct caacctcaga aagatatgat gctgaaaaaa gaagcaccaa agcggattgt
     4861 gagagcaaat ctgaatcccg aaatcaaacg aaaactaact agtcaagaaa tcatttcaaa
     4921 agggaaaagc caatcagcaa gtaagaaaaa ttctttccaa caagtgaaac agaggtccac
     4981 tctgccaaag cagaccaatc aaaaagtaca aaaaagaatg aaccatcgtc caaaatcaag
     5041 gtcaggtgag aaaaaatgaa cttgaagaaa gtgggtgtta tttcattaac ttttctgacc
     5101 atctctttta tgggtatttt gatgtgtgtg ggtttgttgt ttggtgaaga cagtgaggga
     5161 aatggtagtt ctggcacttc tcctggcgga actagtgtct cagaagaagt attgaaacac
     5221 cgtgcaatgg tggaaaagta tgccaaagaa tcggatataa cagagtatat tccaattctt
     5281 ttggcgatta ttcaagtgga atctggtggc acaatggaag acgttatgca aagttctgag
     5341 tcagccggat taccacctaa ttcattaagt accgaggcat ccatcaaaca aggctgtctc
     5401 tattttgcgt ctttggttaa gcgttccaaa gaactagggt gtgatcaaga tagtattatt
     5461 caggcctata actatggtgg cgggtatcta gatcatgtcg ggaaaaacgg gaagaagcat
     5521 agttttgctt tagctgaatc tttttcaaag gaaaaatcag gtggtcaaaa agtcgattac
     5581 cccaacccaa tcgcgattaa ggaaaatggt gggtggcggt ataattatgg caatatgttt
     5641 tactgtttat tagtgaagca atacctaact acaactcagt ttgatgacaa aatagtccaa
     5701 gaaatttttg atgaggcatt taagtatgaa ggaacggcct atgtgtttgg tggctcaagt
     5761 ccagaaacgg ggtttgattg tagtggcttg acccaatggt gttatgccaa agttgacctg
     5821 aaacttcctc gggtggctca ggatcaatat gatgccatga ctcatattga tctaaaagat
     5881 gccaaaccag gtgatttagt tttctttcat tcaacctatg atgccggtac ttatgtgacc
     5941 catgtgggca tttacgcagg ggagaatcgc atgtttcatg caggagatcc agtggggtgg
     6001 acgaatctaa cggaaaacta ttggcggcag catcttatcg gagctggccg atacaaataa
     6061 gagaaaagag gaaaaagaat gaagataaaa attgaacgga atcaaaagga aaagagccca
     6121 aaaaagaaaa aagaacgagt tgtatccgtg ggtaagcatc gaaaaatggt gcttgccctt
     6181 tggcttttat tgtcttgtag tcttgctttt ggcgtttata aaaatttcac cgccattgat
     6241 caacatacaa ctcatgaaaa agtggttata aaagagaagg tagtgaatac ttctggcgtt
     6301 gaaagtttta cagaggattt tgcgaaagaa tatttttcat ggaaaaataa caaagaagca
     6361 attgaaaaga gaatgagtaa tctcgaacag taccttacag aaaacggtct cgctttaatc
     6421 caagatatgg ttcgagcaga tattcccacg agttccgaag ttcaatctgt aaaaattcta
     6481 gatgttgaaa aacactcgga agaatttgtc gtttcatttt tagtggagca acatattaca
     6541 gagggaaaaa agacgcaatc aatttcttct gcatatcgag tgacaatctt tgaagatgaa
     6601 aacaggaatc atttagtaac tagtttgcca acaatgattg gaaagccaga caaggcaaaa
     6661 tatgaagcaa aacaagtaga aaacgattca gaaattgatg ccaaaacaac ggaagaaatc
     6721 accgattttt tagaaacatt ttttaaactt tatccatcag cttctgaaaa agaattggag
     6781 tattatgttg aaaatggtgc gatgcgacca atcaatattg acttaaagtt ttcggagtta
     6841 gagaatccag tttatctaca aaacggaaaa aattttctag tgagtacaaa gataaaatac
     6901 cttgataatg tatcaaaaac cactagctac tttcagtaca aattagtatt acaaaaaaat
     6961 ggaaactgga aaatcgttga tgttaaataa taatgactca atctaatttt attagaagtt
     7021 ataagtagtg taacttattt
//
LOCUS       DADVXN010000120         7032 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.124, whole genome shotgun sequence.
ACCESSION   DADVXN010000120 DADVXN010000000
VERSION     DADVXN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.124"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            205..1062
                     /locus_tag="KB942_002439"
     CDS             205..1062
                     /locus_tag="KB942_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6852826.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1055..1957
                     /locus_tag="KB942_002440"
     CDS             1055..1957
                     /locus_tag="KB942_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6852827.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1990..2340
                     /locus_tag="KB942_002441"
     CDS             1990..2340
                     /locus_tag="KB942_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6852828.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2389..3312
                     /locus_tag="KB942_002442"
     CDS             2389..3312
                     /locus_tag="KB942_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6852829.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3390..4283
                     /locus_tag="KB942_002443"
     CDS             3390..4283
                     /locus_tag="KB942_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6852830.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4321..4884
                     /gene="amaP"
                     /locus_tag="KB942_002444"
     CDS             4321..4884
                     /gene="amaP"
                     /locus_tag="KB942_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6852831.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4898..5086
                     /locus_tag="KB942_002445"
     CDS             4898..5086
                     /locus_tag="KB942_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6852832.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5099..6001
                     /locus_tag="KB942_002446"
     CDS             5099..6001
                     /locus_tag="KB942_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6852833.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6033..6275
                     /locus_tag="KB942_002447"
     CDS             6033..6275
                     /locus_tag="KB942_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6852834.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6531..6902)
                     /locus_tag="KB942_002448"
     CDS             complement(6531..6902)
                     /locus_tag="KB942_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852835.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2561 a         1191 c         1397 g         1883 t
ORIGIN      
        1 tggctatata tagtagtttt tggtcaatac ctttgaataa aaagccatga aaaacgaata
       61 gttagcgctt accgatgcgt tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa
      121 gcatatattt tgctttaaaa ccaaatgagg aatattctag atgtagaaca ggttagaaaa
      181 agattagaaa aaggagtgat tttgatggag aagagatacg gcgtagttcc tgtggtaact
      241 gcttcagacg aaaattatgc tccttacttg agcgtgatga tcgcaactgc attagaaaat
      301 tgcaataaaa caagacgcat aaaattttat gtgattgacg atggtctatc agaatatagt
      361 aaagaaggat tggaagaaac ggtaaataaa tattcaagta atgcaagtat ccaattcctg
      421 acagtggaaa aagatatcta cgaagacttc ctagtcagtg accatatcac aactaccgct
      481 tacttaagaa tttctttacc aaacttatta gctaaagagg attataagaa agtcctttac
      541 ttagattcag atgtgttagt actagacgat atcgtaaaac tatatgatga gccgttaaat
      601 ggaaaaacaa tcggcgctat tatcgatcca ggccaagtga aagctttgga aagattagga
      661 atcgattccg atgacttgta cttcaattca ggagtgatgg tgattgacat tgatcaatgg
      721 aataaaaaag aaattacaga aaaaactatc cattacctca gtgaaaatgg cgatcgaatc
      781 atctatcacg accaagatgc tttgaatgct gtactttatg aagattggga acaacttcat
      841 ccaaaatgga acatgcagac ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa
      901 tatgaacgtc agtataaaga aggaaatgaa aaaccttcga tcgttcactt tacaggacat
      961 gataaacctt ggaatacgtt gaaagatcat ccgtatacca atttatactt aaaaaaatta
     1021 gcacacagta ctctaacgaa agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt
     1081 cgcaagttgt aacacaaaat tcgtaccaca tttagctgca ttatttgtct cagtattaga
     1141 taattgcaac ccgtctaaat ttgttcggtt ctatgtgatt gacgatgata tcgattttga
     1201 aagtaaacag cttttacgct tctcagtgaa aaacgcacga atgaactcag atgtggaatt
     1261 cttgaaaatc aataaagaat tctttacaaa tgtggtaatc agcgatcgta ttccagaaac
     1321 agcttattat cgtattgcga ttccggaatt gttccgcggc acagaagttg aacgtatctt
     1381 atatatggat tgcgacatga tcgctttaca agatatctct aaattatggc gtcttgactt
     1441 cggtgattca atcgtagcag ctgttgaaga cgctgggttc catcaacgac tggaaaaaat
     1501 gaaaatttca gctaaatcaa tgcgttattt caactcaggt ttgatgctga tcaatgtgaa
     1561 aaaatggtta gatgaaaata ttacgcaaaa agtattagat ttcatcgaac ataatccaga
     1621 aaaattacga ttccatgacc aagatgcatt aaatgctatc ttacatgacc gttggttacc
     1681 acttcatccg cgttggaatg cacaaggcta tatcatggca aaagcaaaaa aacatccaac
     1741 agcagcaggt gaacgtgaat acgaagaaac tcgcaacaat ccttacatta tccatttttc
     1801 aggacatgtg aaaccgtgga gcaaggattt tgaagggcca acgaagaaat attacgaaaa
     1861 atatgcaggg atgacggctt tccgttgtgt agctaaattc cctaaatatc caaaatatgc
     1921 aaaagttcaa ccaaaaacag atgtagttgt taaataatag aagaccacaa ctgaagaggg
     1981 ggagtaggaa tggaaattac aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt
     2041 ggggaaaata caaaaatcgc actcgcgctt atcaatgatt ccgatccatt tctaagggac
     2101 aaaggtgctt ttgcaaaagg cagctttttt caaatcattc cttttgtttc ggaatttgga
     2161 gaatatgcga ctaaaatcga gcatccatta ttggacatct atacatctaa attagagcaa
     2221 aattatttcg gtaaacgatt gaacatggac ttcaacaaac agcttgacag cttttcatta
     2281 gaaaatgaaa tcgcagtact cgattataat atcaaattaa agaactgctt cttcagctaa
     2341 tagtttcgag aaagtccaaa aaggaggtat acattgagat actactttgt ggatgaagaa
     2401 aagaatgtaa tagaaacaga tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag
     2461 cccgaagaga ttgagcaagc aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga
     2521 accgaatatc cggaagaagt ttctcggatg gagcgccttt atgagacgaa actagagcat
     2581 ccattttctt tggtagtgat taatctagat cagtcagata atcgtatcga aaaaaaatta
     2641 acgccaatat cttttgtact ttccgataat ctcttgatta cttgtatcga tagaaaaacc
     2701 gaatttgtgg atcggctgct gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc
     2761 atcacgtata cattattaga tatttacacc cattacgtga aagaactaag agagatgaaa
     2821 aagcgaattg acgctttgga taaagcagct cgtaaaacaa cggaaaatga agaactgtat
     2881 aaacaggcag atcttgaaag agacatcgta tatatcgatc ataccttaag agatcaacgg
     2941 gaaacaatgg atcaattatg gaattctgaa actttcatca aacgtttaga cgatgaacag
     3001 ctactctatg atgtccattt gagacagcga caaacagaaa aaatgatcga gatctatcga
     3061 gatttattag agagtattgg tgatctattc aacgggatga tggacaataa tctaaatcat
     3121 atcatgaaat atctagattc agctgcatta atcatttccg ttccggcgtt gttcgcaggg
     3181 atttggggaa tgaatacagg aggattaccg ggtaaaagtt ctgctctagg ctttatactc
     3241 gtagtctttg gctctttgat agcagcagta gttttaggtt accatttata ccggaaagat
     3301 tatacaaatt aaagaatcta tttaaaacag gtatcaaaca aagaaatttg ttttggcaat
     3361 acaatttaaa aactaggagg aatacagcaa tgacagaacc aaaattaatc gacccgcgaa
     3421 acttgtacca tacggagaaa tttcctaaac aagatcagga tacaccagct ttacagcaga
     3481 atatgattcc tgttcctgat tgtggagaag aatcctatga aggcaataat caactagaaa
     3541 accgccgtgt actaatcact ggtggagatt cagggatcgg tcgtgcagca gcgattgcat
     3601 ttgctagaga aggggcagat atcgctcttc aattcttccc aggagaagaa aaagatgcag
     3661 aggaagtagc aaaatacgtg aaagatgcag gacgtaagat tgtcttacta ccttttgatc
     3721 ttcgtgacga acaagcacct aaagaaatca tagataaagc cgtgagtgaa cttggcgggc
     3781 tagatacgat ggtactaaac gcaggacagc aaatttcttg tcagtctatt gctgagttac
     3841 cacttgagca agtcaaagac acatttatga taaacatcat cgctatgttt gctttagtga
     3901 aagaagctgt tccgcatatc ccagcaggag gagcaatcgt aacaacaact tctgttcaag
     3961 cattcaatcc aagcgaacat ttattagatt acgcagcaac aaaagcttca atcgctaact
     4021 ttacgatcgg tttagcaaaa cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac
     4081 ctggtccaat ttggacacca ttacaactag acgaaggaca accttctgat tcgatccctg
     4141 aatttggtca gcaaagttta ctagaacgtg caggtcagcc aggagaatta gcaccagttt
     4201 atgttttctt agcatcgaat aaagcaagtt atgttacagc ccaaatttac ggagtaactg
     4261 gtggagaagc gatcaactta taagaagttg atcgtttcac ccctatgagg aggtgcgaaa
     4321 atgaataaag gtgtaaaaac gatcggagtg atcggaacat tacttttgct atctgtgctt
     4381 ttgtttgcga tactattaag tgcaacctat ccattgcctt ttcagctaga gaagtttcga
     4441 ttcttttcca tcactaattc atacgtgcaa caatatgtat tttggcttgc cgttgcattt
     4501 gctattttga caattatcac gttacttgtt ttcctgtttt atccaaaaac aatcggaaac
     4561 tttctactga aacatggtga tggggagttg acgttggata aaaaagcaat tgaaggaatg
     4621 gttcgttctc acttgcatga agatgaattc atccattcac caaaagtaaa tgtcaaagca
     4681 acgaaaaaca agatcgccgt caatgtaaaa ggggagttga aacgaacttc agcactcatt
     4741 ggtaaaacag gaacattgat gaaagaaatc gaaaatgaag tacaacacat attgggaaca
     4801 aaagagccag tgaaagtatc ggtgaaatac acaggctttg aagaaccagc taaacgaagc
     4861 aatgaccgca aacgagtaga ataggggggg accaaaaatg aaagaactat tttcgacata
     4921 caagctccct atcatctttg gggtacttgg attgatatta gccgttttat ggatcaccgt
     4981 gggctttttc aagaccttat tgatcttgat catggcagcg atcggcgtcg ctatcggttt
     5041 ttatctgaaa caaacgaaaa tcttagatca ctattttaaa tcttaggagg aaacatccat
     5101 gaataacaat aacaaaaata ataacaataa aatgaacaac caaaaaccag tgtctgcaca
     5161 tccagaccca acgacaccaa accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc
     5221 accagtatct gcacatccag atccagtagt ccctaacgtt caacaaggag tagaagagaa
     5281 gaaacatgat gcagaaaagc atgcacctca tcatccagga actccagaat ctaaagttcc
     5341 agaacaacat gatgcaatcg atccttctaa acctgtggaa gagcaaaaac atccttctga
     5401 ccatgcgaaa gcacaagaaa aacttagttc tgaccacaca aacggacaag gaaaacttgg
     5461 ttctgatcat acaaataacc aaggaaagca tgattctgaa caggaagatt caatgcgcgg
     5521 tgagctgaat tatgaagata aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt
     5581 agacggttta ttatctgcaa acggtggttt cttctctaat gtggcaggaa aacttgtgaa
     5641 tacagataat gtaacggctg ggatcgaaac agaagtcggt aaaaaacaag tagcagttga
     5701 tatggatgtc attgtggaat atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga
     5761 agtaatcggc aaagaagtaa aaaatatgac acatttagaa gtgatcgaag tcaacgctaa
     5821 cgtagtggac atcaaaacaa aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa
     5881 agtaactgac gcagcgaaaa aaaccggtga atttgcttcg aatcaaacag ataaagtaaa
     5941 atcagcagct ggaagcggcg cacaaaaagt aaaagaaaat acagaaccac gggttcagta
     6001 ataaggaagt aaaacattag gaggaacgaa acatgggatt tatttggtca cttatcgtcg
     6061 gaggaattat cggagcaatt gcaggagcaa tcacgaataa aggttcttcg atggggatta
     6121 ttgcaaatgt cgtagcaggt ttagttggat cagcacttgg tcaagcatta ttaggttctt
     6181 ggggaccaag tattgcagga atggcaatcg tgccatcatt gattggtgcc atcattttag
     6241 tactaatcgt atcattcttt gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga
     6301 aaaagctgtt cttcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt
     6361 catttggttg ggcatattat cgaagatgca agcttttgaa cactgtttat acggaataag
     6421 aggctgtgac aagacttttg tcacagcctc ttatttatgc gggcaaattc ccaattagat
     6481 ttttaaaagt ttttccttaa atgtgtcttt attgatacag agaagcttct ttaataaaac
     6541 aatgcctccc taaattttct tgagatagga aaggtgaaca tacaaaataa tacgaccact
     6601 ttgaaaaccc agatgatcca caaatggatc gtatgatact gatgaagtaa aaagcaatgg
     6661 atcagaaact caaagagatt gaaaaagaca aggaagaaat aatatctctt ttttactctt
     6721 gtctgtttta gccaataata gctcgtaaag taatctagac aaacaaatag aagtaaacaa
     6781 agtttcgtaa gagcagcaat cgtatcatgt tgctctgcta gacgattgac gagcaggaaa
     6841 aatgttggta atggaatata gatcacgagt aatgaaagaa aaaagtaaac aataaatggc
     6901 atactattcc tcctaattgt gtgagttcgc cctcatttta aaataacgat atttgtttgt
     6961 gaagcttttt cgtgtcgtaa caaatgttat ctaatgtatt tttaaataaa taatcattat
     7021 tatcaaaata at
//
LOCUS       DADVXN010000121         7006 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.125, whole genome shotgun sequence.
ACCESSION   DADVXN010000121 DADVXN010000000
VERSION     DADVXN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.125"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            16..354
                     /locus_tag="KB942_002449"
     CDS             16..354
                     /locus_tag="KB942_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBB6852836.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            341..520
                     /locus_tag="KB942_002450"
     CDS             341..520
                     /locus_tag="KB942_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852837.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(563..1033)
                     /locus_tag="KB942_002451"
     CDS             complement(563..1033)
                     /locus_tag="KB942_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852838.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1120..1818
                     /locus_tag="KB942_002452"
     CDS             1120..1818
                     /locus_tag="KB942_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBB6852839.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1996..2337
                     /locus_tag="KB942_002453"
     CDS             1996..2337
                     /locus_tag="KB942_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852840.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2330..3001
                     /locus_tag="KB942_002454"
     CDS             2330..3001
                     /locus_tag="KB942_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBB6852841.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3007..3699
                     /locus_tag="KB942_002455"
     CDS             3007..3699
                     /locus_tag="KB942_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852842.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            3696..4445
                     /locus_tag="KB942_002456"
     CDS             3696..4445
                     /locus_tag="KB942_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852843.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4457..4726
                     /locus_tag="KB942_002457"
     CDS             4457..4726
                     /locus_tag="KB942_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852844.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            4732..4896
                     /locus_tag="KB942_002458"
     CDS             4732..4896
                     /locus_tag="KB942_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852845.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            4889..5188
                     /locus_tag="KB942_002459"
     CDS             4889..5188
                     /locus_tag="KB942_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852846.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            5188..5490
                     /locus_tag="KB942_002460"
     CDS             5188..5490
                     /locus_tag="KB942_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6852847.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            5530..5895
                     /locus_tag="KB942_002461"
     CDS             5530..5895
                     /locus_tag="KB942_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852848.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            6385..6525
                     /locus_tag="KB942_002462"
     CDS             6385..6525
                     /locus_tag="KB942_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852849.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            6522..6704
                     /locus_tag="KB942_002463"
     CDS             6522..6704
                     /locus_tag="KB942_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852850.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            6717..6911
                     /locus_tag="KB942_002464"
     CDS             6717..6911
                     /locus_tag="KB942_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852851.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         2566 a         1077 c         1481 g         1882 t
ORIGIN      
        1 agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta
       61 attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg
      121 acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt
      181 ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc
      241 tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt
      301 ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc
      361 gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa gcaggcaagc
      421 gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg aagctgaagc
      481 tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag aaaagagaaa
      541 ctagaatgta tattactatt actcagttta attctttcac taatttctct attgggaagt
      601 ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta aatatcattt
      661 ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat gcaaggtaac
      721 aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat attctattat
      781 catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac catctgcggt
      841 ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt gaatagttag
      901 aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta agttcaactt
      961 ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc aatataaaac
     1021 ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata aacaccacca
     1081 gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     1141 aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat
     1201 caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg
     1261 tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt
     1321 agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa
     1381 caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tactgttcct
     1441 caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat
     1501 gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga aattaagcaa
     1561 aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc ttatgaagaa
     1621 atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt ttcaatccca
     1681 agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat tgaaatgtgg
     1741 ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca gagacttttt
     1801 ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg ctaattgctg
     1861 gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc aaaaagcatg
     1921 gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa acacatcata
     1981 aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt gattgatcct
     2041 gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg
     2101 acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac tatttgggat
     2161 catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat ttacgtcatc
     2221 gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat tgatatctct
     2281 tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaat
     2341 caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa
     2401 aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga
     2461 tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca
     2521 tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt
     2581 atctgtctca gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta
     2641 tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg
     2701 ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag gggaagatat
     2761 tccaacacca cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc
     2821 tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca
     2881 gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga
     2941 gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg
     3001 atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga
     3061 aaagacataa gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat
     3121 gacggctacg atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc
     3181 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
     3241 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
     3301 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
     3361 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
     3421 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
     3481 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
     3541 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
     3601 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
     3661 aaaacaatca aaaagttaaa tattggaggg gaaaaatgac agaacatcga agttattatg
     3721 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
     3781 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
     3841 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
     3901 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
     3961 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
     4021 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
     4081 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
     4141 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     4201 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     4261 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     4321 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     4381 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     4441 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     4501 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     4561 aattgaagga attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact
     4621 ctatatcgat ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg
     4681 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta
     4741 aagacattta cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg
     4801 tacgaagaca agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta
     4861 ctcaaattac tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat
     4921 cgaattgact tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa
     4981 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat
     5041 ctggcatggt gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg
     5101 tctgagcaaa aatattattt gggatagagc taggagctta tggattgatt caaaaggaag
     5161 acagtttagg tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg
     5221 gcaaaagata agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa
     5281 gaaatcgggg aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga
     5341 attggcgatg tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac
     5401 gagtgcttgg attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt
     5461 gttttcgtta agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg
     5521 aggatagaca tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt
     5581 gaagcatatg ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga
     5641 gaagaattgg agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa
     5701 tttctttgca ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg
     5761 ggagaatttt tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg
     5821 gtatacaact acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa
     5881 tgggtagtcc gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg
     5941 ccgccattgc tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct
     6001 cttttgaagt gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga
     6061 gatttgcgtc aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct
     6121 aaggacatca tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa
     6181 aaatattcga tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg
     6241 cagtaacaga ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat
     6301 tatgagcgtg gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt
     6361 atctgtttag atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa
     6421 actgctttaa ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc
     6481 tggtccaagc ataagaatga catcattttt ccagaggaac aatgagagca actgatccag
     6541 taattatcct tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct
     6601 tgctcttttc tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc
     6661 ttgatgtcgg attgcttttg ctccatctag cagaaaaaat ttgatttgag gtgaaaatga
     6721 tgattcaact tgcaggcatc caaacaggga aaatttattt ttctggagaa agcaaaagcg
     6781 aagccaacca atggctattg aaaacttaca cgaacaataa gaagctaagg aaaaaatatc
     6841 ctgatttgtt gctaaaggat gatcagatta tgccggaacc gatgattttg acccgtaagg
     6901 agggaagttg aaactaaaaa accagccgtg tgggctggag ttgctatttc tttttaataa
     6961 tgagtttttc taaatttaat tcattctcac gagtttgcgt ccaata
//
LOCUS       DADVXN010000122         6895 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.126, whole genome shotgun sequence.
ACCESSION   DADVXN010000122 DADVXN010000000
VERSION     DADVXN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.126"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..182)
                     /locus_tag="KB942_002465"
     CDS             complement(<1..182)
                     /locus_tag="KB942_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6852852.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="KB942_002466"
     CDS             517..951
                     /locus_tag="KB942_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6852853.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="KB942_002467"
     CDS             974..1294
                     /locus_tag="KB942_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852854.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="KB942_002468"
     CDS             1435..1626
                     /locus_tag="KB942_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852855.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="KB942_002469"
     CDS             complement(1697..1954)
                     /locus_tag="KB942_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBB6852856.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="KB942_002470"
     CDS             2112..2375
                     /locus_tag="KB942_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852857.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="KB942_002471"
     CDS             2399..2587
                     /locus_tag="KB942_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852858.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="KB942_002472"
     CDS             complement(2670..2855)
                     /locus_tag="KB942_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852859.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="KB942_002473"
     CDS             3070..3441
                     /locus_tag="KB942_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6852860.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="KB942_002474"
     tRNA            complement(3591..3663)
                     /locus_tag="KB942_002474"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="KB942_002475"
     CDS             complement(3709..4137)
                     /locus_tag="KB942_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852861.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="KB942_002476"
     CDS             complement(4197..4955)
                     /locus_tag="KB942_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBB6852862.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="KB942_002477"
     CDS             5250..5447
                     /locus_tag="KB942_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852863.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="KB942_002478"
     CDS             5553..6035
                     /locus_tag="KB942_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852864.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="KB942_002479"
     CDS             complement(6093..6689)
                     /locus_tag="KB942_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6852865.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2332 a         1059 c         1355 g         2149 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttg
//
LOCUS       DADVXN010000123         6791 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.127, whole genome shotgun sequence.
ACCESSION   DADVXN010000123 DADVXN010000000
VERSION     DADVXN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.127"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            25..915
                     /locus_tag="KB942_002480"
     CDS             25..915
                     /locus_tag="KB942_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBB6852866.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(988..1812)
                     /gene="nadE"
                     /locus_tag="KB942_002481"
     CDS             complement(988..1812)
                     /gene="nadE"
                     /locus_tag="KB942_002481"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBB6852867.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1824..3296)
                     /locus_tag="KB942_002482"
     CDS             complement(1824..3296)
                     /locus_tag="KB942_002482"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBB6852868.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3933..4295)
                     /locus_tag="KB942_002483"
     CDS             complement(3933..4295)
                     /locus_tag="KB942_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6852869.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4518..5270
                     /locus_tag="KB942_002484"
     CDS             4518..5270
                     /locus_tag="KB942_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6852870.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5410..6333)
                     /locus_tag="KB942_002485"
     CDS             complement(5410..6333)
                     /locus_tag="KB942_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6852871.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6348..6620)
                     /locus_tag="KB942_002486"
     CDS             complement(6348..6620)
                     /locus_tag="KB942_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852872.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2075 a         1352 c         1160 g         2204 t
ORIGIN      
        1 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
       61 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
      121 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
      181 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
      241 tcgagccttc gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt
      301 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
      361 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
      421 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
      481 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
      541 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
      601 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
      661 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
      721 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
      781 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
      841 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
      901 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
      961 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
     1021 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
     1081 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
     1141 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
     1201 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
     1261 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
     1321 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
     1381 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
     1441 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
     1501 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
     1561 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
     1621 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
     1681 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
     1741 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
     1801 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
     1861 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
     1921 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
     1981 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
     2041 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
     2101 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
     2161 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
     2221 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
     2281 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
     2341 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
     2401 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
     2461 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
     2521 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
     2581 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
     2641 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
     2701 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
     2761 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
     2821 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
     2881 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
     2941 ccttccactt ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
     3001 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
     3061 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
     3121 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
     3181 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
     3241 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
     3301 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
     3361 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
     3421 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
     3481 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
     3541 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
     3601 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
     3661 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
     3721 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
     3781 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
     3841 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
     3901 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
     3961 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
     4021 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
     4081 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
     4141 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
     4201 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
     4261 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
     4321 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
     4381 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg
     4441 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
     4501 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
     4561 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
     4621 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
     4681 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
     4741 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
     4801 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
     4861 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
     4921 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
     4981 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
     5041 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
     5101 aagctcataa gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag
     5161 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
     5221 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
     5281 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
     5341 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
     5401 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
     5461 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
     5521 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
     5581 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
     5641 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
     5701 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
     5761 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
     5821 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
     5881 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
     5941 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
     6001 aaattcttca agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac
     6061 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
     6121 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
     6181 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
     6241 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
     6301 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
     6361 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
     6421 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
     6481 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
     6541 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
     6601 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
     6661 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
     6721 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
     6781 tattagtgaa t
//
LOCUS       DADVXN010000124         6765 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.128, whole genome shotgun sequence.
ACCESSION   DADVXN010000124 DADVXN010000000
VERSION     DADVXN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.128"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(69..251)
                     /locus_tag="KB942_002487"
                     /pseudo
     CDS             complement(69..251)
                     /locus_tag="KB942_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            238..435
                     /locus_tag="KB942_002488"
     CDS             238..435
                     /locus_tag="KB942_002488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852873.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(478..1053)
                     /locus_tag="KB942_002489"
     CDS             complement(478..1053)
                     /locus_tag="KB942_002489"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6852874.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1208..1734)
                     /locus_tag="KB942_002490"
                     /pseudo
     CDS             complement(1208..1734)
                     /locus_tag="KB942_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2191..3702
                     /gene="gadC"
                     /locus_tag="KB942_002491"
     CDS             2191..3702
                     /gene="gadC"
                     /locus_tag="KB942_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBB6852875.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3715..5115
                     /locus_tag="KB942_002492"
     CDS             3715..5115
                     /locus_tag="KB942_002492"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB6852876.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5280..6715)
                     /locus_tag="KB942_002493"
                     /pseudo
     CDS             complement(5280..6715)
                     /locus_tag="KB942_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2085 a         1168 c         1222 g         2290 t
ORIGIN      
        1 tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
       61 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      121 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      181 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg
      241 aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact
      301 actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta
      361 cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa
      421 cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca
      481 atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac
      541 aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt
      601 tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa
      661 tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac
      721 tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa
      781 ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa
      841 ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg
      901 actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct
      961 cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt
     1021 cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc
     1081 aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta
     1141 taacatctac tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc
     1201 tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc
     1261 gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt
     1321 agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc
     1381 tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt
     1441 gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg
     1501 cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt
     1561 acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt
     1621 taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt
     1681 caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa
     1741 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt
     1801 ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa
     1861 gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg
     1921 gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag
     1981 atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg
     2041 aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa
     2101 agaggtagac aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg
     2161 acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt
     2221 ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca
     2281 tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt
     2341 gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt
     2401 tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt
     2461 caaattacag taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta
     2521 attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt
     2581 ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa
     2641 gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc
     2701 tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac
     2761 ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta
     2821 gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca
     2881 atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca
     2941 gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg
     3001 cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt
     3061 gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt
     3121 gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt
     3181 ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact
     3241 tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt
     3301 tatctcgtag gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa
     3361 gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga
     3421 attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt
     3481 gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt
     3541 cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa
     3601 cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag
     3661 caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta
     3721 tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt
     3781 gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct
     3841 tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact
     3901 ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa
     3961 aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat
     4021 atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc
     4081 ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt
     4141 gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt
     4201 taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca
     4261 atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac
     4321 acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag
     4381 ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt atacattcat
     4441 gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat
     4501 tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat
     4561 ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt
     4621 aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca
     4681 gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat
     4741 attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt
     4801 atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa
     4861 gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg
     4921 caagtcccag cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt
     4981 gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca
     5041 attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac
     5101 ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg
     5161 agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat attcatttgt
     5221 tacactctca atcttagctg ttgttctatt catcattact cttattgtaa gtagttcagt
     5281 caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt aatcggatat
     5341 ataattgttt tctcactacc taagccttct tggtataaag aactatcgca aagtataatc
     5401 cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca aatgatatta
     5461 ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta taaataccta
     5521 aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg ctcatttaaa
     5581 agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag aaataaattg
     5641 ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc gcgttgctct
     5701 tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca aagtgagcgg
     5761 ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat attcagagat
     5821 aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg gttttgataa
     5881 acaacgttca agtctttttg taacaattca aagttatcgc atagataatt tctagagttg
     5941 aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa agaaagttgc
     6001 tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct tttttcttcc
     6061 tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat acctaataaa
     6121 ataaattgtt tttcaactgt agggtaactt cttgaaaaaa atgtttttct acataatcaa
     6181 tcaattgttg accgctttga ataattgatt tatttagctg gatcaactct gtccgatttg
     6241 tctcatagat tatcgttagt aacaaacttc gataatcctt ttcaggaatt tcaaaatggc
     6301 catcagcatt taagtactgc atttgtttaa aaaaattaat caactcattt cgtaagttgt
     6361 aaactttgct taacgaaatg aaggtagtat cagctatatt actccaattg tgttcccctg
     6421 ataaataatg tcctagtaca ttgaaaaagt cagagcgctg atagatgagt tgagtgagtg
     6481 ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct acttctagat ttctccactg
     6541 aaataatttg ctgatggagt atgtcggtaa tgcattccaa gtcgttagtg acagtgagtg
     6601 ggcaaacgtt caattgatct gctatttcgc gaatctgaat ggtccctttt tcgtgtaatt
     6661 gctcacaaat gtaaacttga cgaaaaatac ttttctccag ataggtttcc aacatagtac
     6721 tcctcctagt cattttatac ataactatta taactctacc aatcg
//
LOCUS       DADVXN010000125         6625 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.129, whole genome shotgun sequence.
ACCESSION   DADVXN010000125 DADVXN010000000
VERSION     DADVXN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.129"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            141..2816
                     /locus_tag="KB942_002494"
     CDS             141..2816
                     /locus_tag="KB942_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBB6852877.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2998..3237
                     /locus_tag="KB942_002495"
     CDS             2998..3237
                     /locus_tag="KB942_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852878.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3253..4626
                     /locus_tag="KB942_002496"
     CDS             3253..4626
                     /locus_tag="KB942_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6852879.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4630..6264
                     /locus_tag="KB942_002497"
     CDS             4630..6264
                     /locus_tag="KB942_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6852880.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6427..>6625
                     /locus_tag="KB942_002498"
     CDS             6427..>6625
                     /locus_tag="KB942_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852881.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2321 a         1071 c         1330 g         1903 t
ORIGIN      
        1 ttccaaaatc acaacttttt tcttttgtca agattttgag tgggatatat ttttgtagaa
       61 ctgaagatca tgaaattatg agaggttgga ggtaatgtag tcatacaaat aaaaattttt
      121 tagaaagtga gtggtaatta atgaaaatga atcacacttc aattcagaag catttacaac
      181 tgttaaatac actggggatt tcttatgtta atttgagaac tttttctaca ttaaacaaga
      241 aaaccgtttc tcatattttt tctactgcag attctcaagc aattattaac tatttagaaa
      301 aacagaaatt gtctgaaggt acagtaaaca taacttataa tcctattaaa caagaggtga
      361 ttacacaaaa aaagcattct gtgagagaca atgatattga aaaaattcgg tttgtattta
      421 ttgatattga ccctaaaagg aaagagggaa gtgcaacaga tagcgaaaag aaaaaggcag
      481 aaaatgttat ggaacaagta gaacgttatt tgaaagaaaa gagaatagaa tcatttgtaa
      541 aagttgatag tgggaatggc taccatattt ttcttcccat aaatgaacaa ccaaataatc
      601 acgagacgat tcttactatt cagaattttc ttcaattact tcaccgaaga tttggagtag
      661 aagatgaagt tgatattgat acttctgttt ataatccttc aagattatgc aaattagttg
      721 gtacaccagc ggtcaaagga aagtccacaa aagagcgtcc gcatcgttta tctagtttcc
      781 tttctattcc cgataacata gatagaagta attgttttaa aattcttgaa cagatagttg
      841 aagaaaattc catgatgtca acctctagag taaaaagcca tccatctttt agagaagtgt
      901 taccttttat tcaagcagac tataaacaat ggttagctca ctatcctgaa ttaacttata
      961 ccataaaaga gggcgatcat aaaggagtta ccttacttat atttgacgaa tgtccattaa
     1021 gaaaacacac gaataattct aatggttctt gtttgtctgt tacggctgat aataagattc
     1081 gatttcattg tctgcatgct tcggataaaa ataaagatat tcaagacttt gtaaaaaaat
     1141 atccgttgcc aaaacctgta aatccttctg tagaatcatt agaatccaat caagaatatt
     1201 attttgacca gtttaaactg aatcaagaag gagtattttt ggtgaaagaa ggtcaagaag
     1261 aaaaagttgc ttgtccgatg tttattaagc aaatacgcaa aaatatcgaa caaaaagtca
     1321 ttacacttgt tcttgcctat tatgtcgatc attgttggga tgaaatagaa atagaaggtg
     1381 gatgtttaca aactaataat tttaaacaat tagcaaaaca tggcttaatc attaaagcta
     1441 gaagagaagc tgaagtcgtt gactttttac agattcaacg ggagaatgct ccaatctttt
     1501 ttgaacatga atctcttggc tggaaaaaag aaaatgaagc aatgattttt cagttacaag
     1561 attcttttgg acgtgaaatt ccttctgtat taactaaaaa ctcatgctac caattaacaa
     1621 ctaatggtac agaagcggaa tttgacgcat tggttcataa gtatgtttta ggaacgaact
     1681 tagaacttgc ttggtcgatt ggtattgctt ctattgtgtt aggctattta aacgttacga
     1741 aggctaatac ttttttgagt ttactaatta acctacaggg caagagcaca acgggtaaaa
     1801 ccactacgtt acactttatt gcctctttat ttggaaatcc aactacggtt cttagaaata
     1861 tgaatgctac caataaagcc attataaagc tagcatcaaa caatcaggga ggtgttccac
     1921 taatactaga tgagttagga gcagcaactc aaaataactt tagctcgttg atctatcaat
     1981 tagcttcagg agaagaaagg ctaagattag attctaattg tcagcttatg ccacaagaga
     2041 ctttttctgt tttgaccctt acctcttctg aagagcgttt ggaaaattat ttcaatgaga
     2101 aattaactgg tttgaaagtt cgatatatcg aattttctga tatcaagttt acaaaggatg
     2161 cgaatcacgc agagaaagtt aaaagtcttt cttctaatac ttttggttgg ggtataaagt
     2221 tatttctttt gaaactcttt gaacaaggga ttacaaaaat tgaagcaact tttgtcgaag
     2281 ctcaaaaaaa actatgggct gttttacctg aacattcttt gaagcaacgc attggtaata
     2341 atctagcttt gatttatagt ggagccctcc taattcaaga tatactgggc tatcctttac
     2401 gattagagca tatacagacg gtgcttatta attcgtacga acagttaatt gtagatacta
     2461 gtttaggaag tgagcaaacg gtatttgacc gagtgaaaac attgttggtg atgaatagcc
     2521 ataaattttt aacaaacagt gacaacaaac atacgcccag tacgatttgg ggaaaagtat
     2581 caattcaaaa aaatggggat attaaggtaa atatttttcc tatcccgttt gaggacttgt
     2641 taaggcgaga atttcaagta aaggatctaa cctatctttt taggcaattg cttgattcgg
     2701 gtaatatgtg tgcagaaaaa gggagaagaa cgaaacgtat tcacatgaat cgtcaagttc
     2761 ttccaacata cgagattcta ttaccttctt cactcaaatc gtattttcga ttgtaattta
     2821 gaataaacca aagtatgaaa gtgaggcacc atgttatgga aatatttctt attgatttag
     2881 caaaacaact gattacttat ctgtatgata gcgagcaaac ccaaacggaa gtaaatgaag
     2941 cttgatctat gcttcattta cttcattcaa tcaaacatga aagaaaggaa aattctaatg
     3001 actaaaaata aaatgacact aaaagcagag gttcttctgt atattcaaga acatttttca
     3061 aatcaagcct tttttacaaa accaatttac ttggatttcg aaataagagg ggtatctgct
     3121 ggatctatcg ggggtactct tcaagcgttg aagaacgaag gatatttaga aaatcacttt
     3181 gtgcaacgtt cgttcaatag aagagtcgtg aaaaaatggt atttagttca ttcatagaga
     3241 ggggaatcaa gcatgcgtgt ggcttgttat gatcgaacgt ctgtttatga agaagcgaaa
     3301 attagtaatg acgctcaata tgatgcagtg aaagagtatg ttttagagaa atatgaacaa
     3361 attccccaac gattttcaga caaaggagaa tcaggggaaa aagcggagcg tccagctttt
     3421 cgacagctta gagcttgggt ttgtgcgtca gaagaagagg gggtgatcta tgtttggcgt
     3481 tatgatcgat tggcacgtca agccaaaata gccttagaat ttattgagtt gtgccaacaa
     3541 catcaggttc aagtgtattc gatttctgaa cctcttcctg cttctttctc acacgcgtca
     3601 acgaataaac tatttgtgca attattgttc acttttgcag aacaacaacg aaacgtcatt
     3661 attgagaatg ttcgtgccgg actttcctat aaaaagcaac aacggcaata tttgtcttct
     3721 gcggtaccat atgggtatcg tttagtgaat gggaaaatcc aacaagaagt acacgaagca
     3781 aggattgttc gctatatttt tcaactttat ttgaccaaaa aatatggcta caaaaagctt
     3841 tgtcaacgat tgacgcaaca aaagcttttc ttccgcgaac gtccttttca gccctatcat
     3901 atttatagta tcctaaaaaa tccattgtac tatggcgaaa tcaaaggagg atctttgggg
     3961 aagtatctag gaacctttga accgattctc tcgaaagcaa cctttttaca agcccaagag
     4021 attcgccaat caaggtgtac agctaaaaaa gatacgtatc tgtacttatt acgtcaaaaa
     4081 attcggtgtc ccttctgtgg tcgtcatctg agttcgaagt atcaatggaa tacaaagaag
     4141 acgaagacgc ttcattatta tcattgtacc gaccgaagct gccaaggaat ttttcttcgg
     4201 gcagaaaggg ttgagtcaga agtattggaa aagatacagg ctttttttca atacgaaggc
     4261 gtctatcaag ccttgattgc ccaattaacg caacaattag ccgaaaagcg aaaagatgaa
     4321 aaacgcaaag taatccaaca aaagaaacaa gaagcacaat ggattaaggc atttgaagag
     4381 ggcaattgtt cactggaaga actcaagcaa caactgcaag ctcttcaaaa gccgatgtct
     4441 acttcttcgt ttctttcact ccaacagtgt gaagcaaaag tgaaagaatg gcttcaacta
     4501 aaagacctct ctagtcgaca attattatgt gactatgttt gtcggattga ggtaacaaag
     4561 gataaaacag tttgtgggat ttacttccga caactgacac aaaatatttt agaaaggaac
     4621 gaatgatcta tggaaatgat acaaaccaaa cgagtcgcat tgtatattcg cgtgagtaca
     4681 caagaacaag ctttagaagg acatagtatt caagcacaag aacaaaatgg acgacagttt
     4741 gccgaacgga tgggctatga agtggtagaa gtttatgccg atgaaggaat cagtgggaaa
     4801 tccacaaaaa atcggttcgc ctatcaacga atgatgaacg atgctcggaa ccatcgtttt
     4861 gatctactag tgatttggaa attgacccgt ctcggacgca atatgttaga tgtcttacaa
     4921 acagtcgagg aattattgcg atatgacgta ggattacatt cgatctcgga acaatttgac
     4981 gtgaccactt cgacaggtaa attgatgtta caactccttg ggagtttcgg tgaatttgaa
     5041 cgcaaccaaa ttgcggagaa tgttcaaatg tcaatgcaaa gcttagtgaa agataaaaag
     5101 cgttatgcag gaggccgacg tttaggctat gtcagtggta ttgacacgaa tggtcaaaaa
     5161 caattggtca ttgaaccaga agaggcgaaa attgttcgtc tgatctatgc aaaatattta
     5221 ggcggagcag ggtatcgagc gattgccaat tttttgaatc gtcaaggata tcaaacagtc
     5281 aaaaagaatg ctttttctac cacggcagtc aaagatatct tacgcaataa aatttatgca
     5341 gggtatttag aatatgcgcg ctacatcaat tgggaaacca aacgacggaa ggggaagaat
     5401 ccgagaccga ttttagtgaa aggaacccat gaaccgatta tttcggaaga agattatcaa
     5461 aaagtacaag aacgccttgc tttagaagga aaacaaccca aatggaacca tacaggtgaa
     5521 aatgtattga caggcttgtt acgttgtcca gaatgtggcg caccgatggc agcgagtaat
     5581 gtcacaaata ccctaaagga tggcacaaag aaacggattc gatattattc ttgttcagtc
     5641 tttcggaata aaggagcaag tgtgtgtcat gccaattcga ttcgtgccga acaagcggaa
     5701 acatttgtcg ccgaccgatt aaaggagatc gttcagctac cagaagttct tccccgattg
     5761 gtggcagcgt taaatgaaga aatagtgcga caaagccaac cattggaaca agaactcgtg
     5821 gtgttactgg aacgaaagga agaattgaag actaaaattg aaaaatggga agctgctttg
     5881 gaagatagtc ctgaattgtt tccgatgtta aaagaccgct tagacgaatt gactgaaaaa
     5941 cgacgacagt tgcacattcg agaaaatgaa atcttaggga tctttcaaca aggagagccg
     6001 attcaagtca aagatgtcca acggatattg actagtttgg atcgttttct tgcccagagt
     6061 gagaagaagc aaatcaaagc cttgtatcgt acctttatag agaaaataac ctttgatcct
     6121 aaccataagg agatgttgga aattacgatg aaattcacgc atgccgtggt acaacaattg
     6181 aatgagcaat atcaaatagc cgtatccaaa acaaaggata cggctatttt tgtgttaaaa
     6241 acacccttta cgttaacgat ctagtctagg ttatatcaaa caacgttaga ggggcgctaa
     6301 cgcttctcta gcgatttgaa atcgttagag aagtagttag ggaggtcctt atcataaata
     6361 gggatattta ttaatgaata tttgaaatat aattgtatat aagaattaaa aagagtgggg
     6421 atagatatga aaaagatggg taagtgtatt ttatgtgaaa aatacactga acttactaaa
     6481 gaacatgtac cacctaaaaa atcagggaat actggaggta aaaagaccag aactggtaat
     6541 ttagatgatt tccttaagag cgattttaca aaaggtgatt ttccgaaggg gattaaaagg
     6601 aaacctcaag gaaatgttta ttata
//
LOCUS       DADVXN010000126         6484 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.130, whole genome shotgun sequence.
ACCESSION   DADVXN010000126 DADVXN010000000
VERSION     DADVXN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.130"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            79..852
                     /gene="sufC"
                     /locus_tag="KB942_002499"
     CDS             79..852
                     /gene="sufC"
                     /locus_tag="KB942_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBB6852882.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            865..2151
                     /gene="sufD"
                     /locus_tag="KB942_002500"
     CDS             865..2151
                     /gene="sufD"
                     /locus_tag="KB942_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBB6852883.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2148..3383
                     /locus_tag="KB942_002501"
     CDS             2148..3383
                     /locus_tag="KB942_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6852884.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3370..3840
                     /locus_tag="KB942_002502"
     CDS             3370..3840
                     /locus_tag="KB942_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBB6852885.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3845..5236
                     /gene="sufB"
                     /locus_tag="KB942_002503"
     CDS             3845..5236
                     /gene="sufB"
                     /locus_tag="KB942_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBB6852886.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5334..6476)
                     /locus_tag="KB942_002504"
     CDS             complement(5334..6476)
                     /locus_tag="KB942_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6852887.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         2064 a         1260 c         1395 g         1765 t
ORIGIN      
        1 agtaaggaag gtcattgcgc ttcattctca attagtctaa aatgagtgag aacgaaagag
       61 aatttggggg tataatgcat gtctgtctta gaaataaaaa atctacatgt gtcgatcgag
      121 gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc
      181 atgggaccta acggcacagg aaaatctacc ttatctgccg ctatcatggg caatccaaac
      241 tatgaagtaa cagaaggcga aattttgttt gacgggaaaa atgttttaga cttggaagta
      301 gatgaacgtg cgcgacttgg cttattttta gctatgcaat atccaagtga gataccaggt
      361 atcacgaatg cggaattcat gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa
      421 atttctgtta tggcattttt gaaaaagtta gatgaaaaaa tggctttact aaatatgcca
      481 gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat
      541 gagatcctgc aattgttgat gttagaacct acatttgcaa ttttagacga aatcgattca
      601 ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac
      661 aatttcggtg cattgatcat cacccactat caacgattat tgaactatat cacacctgat
      721 gtcgttcaca tcatgatgga aggccgagta gtcatgacag ggaatgctga cctagctaaa
      781 cgtctggaag cagaaggcta cgctggtatc agtaaagaat taggaatcga ctataaagaa
      841 gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg
      901 gtaaatgatt tttctttaag taaaggcgaa cctgattgga tgagaacctt ccgtcaagac
      961 gcactagcga aagcagatga actgccattg ccgcatatcg atcgggtgaa attccatcgc
     1021 tggtcgttat ttgatgtgaa agaaacacaa accatcagtg aaacaggcac gattccggca
     1081 tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag gttcatggac gatttttgaa
     1141 caactgcctg tggaattagc agaaaaagga gtcattttta ccgacttgtt cactgcaatg
     1201 atcgaatatc cagaactagt ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa
     1261 gaccaattga ctgcactcca tgtagctttt atgaacagcg gtattttcct gtatgtgcct
     1321 aaaaatgtcg tgatcgatga accactcgag tccttattca ttcaagatgg cgcatccgat
     1381 gagcacttct tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt ttcttatctg
     1441 gaacgtttcc agacaaccaa agaacaagta gcaaaatcat caggtaatat catcgtggaa
     1501 gtcatcgcta aagctggttc aaaaatcaaa tattcagccg tagatcaatt aggagaaaat
     1561 attacttctt atatgaatcg ccgtgggcac attttacgag atgcttctgt cgactgggca
     1621 attggtgtca tgaatgacgg tcatgtgatc gctgactttg actcagattt agcaggagaa
     1681 ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg gcagacaaat ccaaggaatc
     1741 gatacacgag taaccaataa agcccctcat acgatcgggc atattttgca acacggagtg
     1801 atccgagaaa aaggaacatt gacctttaat ggtatcggcc acatcttgaa aggggcaaaa
     1861 ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat
     1921 gcgaatccta ttcttttgat cgatgaaaac gaagtaactg ctggacatgc tgccagtgta
     1981 ggacgagttg atccagaaga aatgtactat ctgatgagta gaggattgca taaagaagaa
     2041 gcagagcgct tagtgattcg aggattcctc ggttctgtct taacagcaat ccctgtagaa
     2101 caagttcgga aagaattagt cgaagtaatc gaagggaagt tgaatggatg atcgatgcta
     2161 aaaagctacg caatgatttt cctatcttat ttcaagacgt caatgatgaa cctttagttt
     2221 atctggataa tgcagcgacg acccaaaagc caaaacaagt actccaggca ttagcgtact
     2281 attacgaaca cgataacgcc aatgttcatc gcggtgtcca tacgttagca gaacgcgcga
     2341 cccatgatta tgaacaagca agagaaaaag tacgggcgtt catccatgca aaagaaacag
     2401 cagaagtgct gtttactcgt ggtacgacga caagcttgaa ttgggtagcc agaaccttcg
     2461 gcgaccgcta tatcaaagca ggagacgaga tagtgctctc ttacatggaa catcattcta
     2521 atgttatccc gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac
     2581 taacagaaga aggcttcctg gatatggagg atgcagcgaa aaagattacg gacaaaacca
     2641 aaatcgtctc cattgctcat gtatccaatg tcttaggtgt cttcaatcct gttcaagaat
     2701 tagctgaact tgtccatgaa aacggcgggg tcatcgtagt agatggtgct caatctgccc
     2761 cgcacatggc cattgatgtc caagcactag actgtgattt ctttgctttc agcggacaca
     2821 aaatgtgcgg accaacaggt attggtgttt tatatggtaa acgaaaatgg ttagaagaaa
     2881 tggaacctgt tgaatttggt ggcgaaatga tcgattttgt cgaattatac gacagtacat
     2941 ggaaagaact accttggaaa ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat
     3001 taggccatgc aatcgattat ttgcaaaaac tcggtatgga caatatccat cgatatgaag
     3061 aagaactagc cgcttatgtg ttgccaaaat tacaagcaat tgatggattg actgtttatg
     3121 gtccacaaga cccgaatttt cggacaggag tcgttgcatt caatttggat gggttacatc
     3181 cccatgatgt cgcgacagct ttagacatgg aaggggtagc cgtcagagca ggtcatcatt
     3241 gtgcgcaacc gctattgaag taccttgaag tatcagcgac tgctcgcgcg agcttttatt
     3301 tctacaacac aacgcaagac gccgaccgtt tagttgaagc aatcatggca acaaaggagt
     3361 ttttccaaca tgggactatc taaattagac aatttatacc gtcaagtgat cttggatcat
     3421 tcttcccatc cgcatcatcg tggaagcttg actgactcga caagcaagat cgaattgaac
     3481 aaccccacat gtggagatgt gatcgaattg caagtcgatg taaaagacgg aatcatcaaa
     3541 aatatcgcct ttgacggcag tggctgctcc atcagtacgg caagtgcctc gatgatgaca
     3601 gacgccgtca tcggaaaatc aatagaagaa gcagaacatt taactgtaga ctttctacag
     3661 cttgtccaag gaaaagaagt caaagatccc gaccaattag gcgatgctgc tatgcttggt
     3721 ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa agcactagga
     3781 aaagcaatgg aaaacgatgg taccgcagct ttccatcatt accacaagga agaggagtga
     3841 gaacatgggc gtaccagaat tagaagaata taaattcggg tttcacgatg atgtcgaacc
     3901 ggttttcagt acaggagaag gcttgacaga agaagtgatc cgtgagatgt caagaatcaa
     3961 aggcgaacca gaatggatgc ttgaattccg tctgaaatct ctagaaacat ttaacaaaat
     4021 gccaatgcaa acatggggcc ccgacctttc agacatcgat ttcgatgcca tcaaatatta
     4081 ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga
     4141 tacatttgaa aagattggta taccagaagc tgaacgagct tatctagccg gtgcttccgc
     4201 acaatatgaa tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa agttaggaat
     4261 cgtctttact gacactgact ctgctttgaa agaataccct gaattattca aagagtactt
     4321 ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg ctgaattcag ccgtatggtc
     4381 aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc gatgttccat tacaaacgta
     4441 cttccggatc aatgcggaaa acacggggca attcgaacgt acgctgatca ttgttgacga
     4501 aggagcaagc atccactatg tcgaaggttg tacggcacct acttattcaa gtaacagcct
     4561 gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg tattgccgtt acacaacgat
     4621 ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag catacgaagg
     4681 cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa acaacgatga aatacccaag
     4741 tgtttactta gatggaaaag gtgcacgagg caccatgctc tccatcgcct ttgcaggagc
     4801 aaaccaaatc caagatacgg gtgcaaaaat gatccacaat gcaccaaata catctagctc
     4861 tatcgtctct aaatcaattt caaaagacgg cggagaagta aactatcgag gacaagtaac
     4921 ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga tcatcatgga
     4981 cgaatggtcg aaatccgata cgatcccatt caatgagatc cacaacagcc aagtctcttt
     5041 agaacacgaa gccaaagtct caaaaatctc agaagaacaa ctctactatc tgatgagccg
     5101 aggcctgacc gaatctgaag caactgaaat gatcgtcatg ggctttgttg aaccattcac
     5161 caaagaactt ccaatggaat atgcagtcga actcaatcga ttgatcagtt atgagatgga
     5221 agggtctgta ggttaataac tatttcattg ctcgaaacgt tgatattaaa gcgtttcgag
     5281 cgttttttta tttgtatttt aatggttggc tacgtttttg gctacgtttg gaattattcg
     5341 cccataaatt ttgcaaatcg gtcaccagtt ttcttaacag attgtggtgt gacatgagca
     5401 tagatattca tggtagtttt aatatcttta tgacctaatc gttcctgtac ttcttttatc
     5461 gacgcaccac tttcaaacaa taagctacag tgggtatgcc taaatccatg tggagtaatt
     5521 tgaggctcca tcggtgtctt tttgtaaatc cagtctaacc aatcgtttac tacttggggg
     5581 tagtacaact tgtttgtgtt tgtagtgaaa agaaactgtt ttggtgaatt cgtgttatag
     5641 ccatataaga tcattatttt catttgttct tgttgccaaa tacgcagttg tttaagtgta
     5701 aaatcatcaa gctgaatcgt tcttgatgaa tttttagttt taggtacttg tagaactact
     5761 tgattaaact cgtcttgagc gatagtttta ccaattgtta ttgttttatt gaaatagtct
     5821 atatcttccc attgaagcgc caatgcttcg cttttacgca ttcctgtata agctaataaa
     5881 cggaaaaaag tgtacagttt aactgaatta ttttctttag tattctgtaa aaacagttgt
     5941 aattgatctt tcgtgtaaaa cttcaagggt tgttcttctt caatttttct tggaagcaat
     6001 gttttagaca taggattatc cttacagact tccattgcta ctccaaattt aaatatttga
     6061 gcagttgttt ttctcaagaa accatattgt tttaatgttt tgtgccattc attcacctgt
     6121 ttttggcaat agctaacact tattttatcc aatcgtaatt ccccaaaagc aggtaatata
     6181 tggtttttgc aaaaacgttg agcaacggca acagaagacg gttttactct tgttctgtag
     6241 ttatcaatcc ataattcata caactcttga aaagttgtaa cattgatttt aaactcttgt
     6301 ttttctttaa agtctacaag tagtctatta agtgctgttt gcgcctcagc ttttgatttg
     6361 aatcttctac gctttgtacg tatatctttt ccagtcttgg gatctttccc taaataagtt
     6421 tcaaatcccc aatatttttc gccattttgt aatttataac taaaaaatgt tgccatttga
     6481 ttct
//
LOCUS       DADVXN010000127         6421 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.131, whole genome shotgun sequence.
ACCESSION   DADVXN010000127 DADVXN010000000
VERSION     DADVXN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.131"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(5..250)
                     /locus_tag="KB942_002505"
     CDS             complement(5..250)
                     /locus_tag="KB942_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852888.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(267..1118)
                     /locus_tag="KB942_002506"
     CDS             complement(267..1118)
                     /locus_tag="KB942_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6852889.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1284..2288
                     /locus_tag="KB942_002507"
     CDS             1284..2288
                     /locus_tag="KB942_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBB6852890.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(3071..3628)
                     /locus_tag="KB942_002508"
     CDS             complement(3071..3628)
                     /locus_tag="KB942_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852891.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(3807..4418)
                     /locus_tag="KB942_002509"
     CDS             complement(3807..4418)
                     /locus_tag="KB942_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852892.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(4531..4788)
                     /locus_tag="KB942_002510"
     CDS             complement(4531..4788)
                     /locus_tag="KB942_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852893.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(5385..5663)
                     /locus_tag="KB942_002511"
     CDS             complement(5385..5663)
                     /locus_tag="KB942_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852894.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(5697..5948)
                     /locus_tag="KB942_002512"
     CDS             complement(5697..5948)
                     /locus_tag="KB942_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852895.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1990 a         1232 c         1021 g         2178 t
ORIGIN      
        1 ctttctattt tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat
       61 gttcgtgcca gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga
      121 tttgatcatg tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa
      181 agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga
      241 ttggatccat aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg
      301 ccgactacac catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca
      361 aataattcag tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat
      421 tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg
      481 atttgtttca tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg
      541 aggatcatat catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca
      601 aggaaaactt gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg
      661 tggggtaaga aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca
      721 aatgctcgcc atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt
      781 ggccagtgga tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca
      841 aaagaagaca tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat
      901 atctcttctc taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg
      961 tatccttggg cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa
     1021 acagcgacat ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa
     1081 ccatttgata gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc
     1141 gttcaactat aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg
     1201 aaatttttgt ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa
     1261 ctaagacaga agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta
     1321 ttgacagaga aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat
     1381 atattcgtga ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg
     1441 gacaactagc agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg
     1501 tggaagcaag tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag
     1561 cacctagtgc agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt
     1621 tattgaaagt caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga
     1681 tcgacaaccg cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg
     1741 aagcattcgc tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt
     1801 tttctggcaa aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt
     1861 ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg
     1921 agacagaaga atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag
     1981 aaaaaggtcg gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg
     2041 caaaaatggc ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg
     2101 taaaagaagt acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa
     2161 acgggatcga actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg
     2221 tgaataatcc ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc
     2281 gtttataaaa atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg
     2341 tgtcaagaga aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa
     2401 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
     2461 cttatttctt ggagttaaac gcttctgtcc cagcctcctt attccgaata aatggtgtcc
     2521 aaaagcttgc atctgccaga ttatctcatt tcttatgaca agctaagcaa attatctcca
     2581 ccaaataatt tattcatatt tttgacttgc gcatactaac taattcttaa attttcatac
     2641 aaaatttctc aatttccctt aacgagtaat atttatgtac ggtgattctc actagtatcc
     2701 caatcacttt ttaagttatc ccgatttcaa ttctacttca aaaatagttg cttttatgta
     2761 tgctgttagt tgattagatg gtcatttgtt gagctaggtc gtttatttta aacaagttgt
     2821 ttctttagct tatacctatc tcgtctacca aacgcaagat ggcaaacaca aatgcatacg
     2881 catctgtatt tgccactttg attcgtataa agctaacaac tattaactcc tctcagctaa
     2941 tagttgctct cttttttatt cagattcatt tttgctgatc acagcctagt atttcaatgg
     3001 ttttgaacac taaaccaaat tgttccatta caacattcac tggctgatga aatggattag
     3061 tagatttcga ttattcaaag cgattttcta ttggtggatc gtgttcccaa ctaataagac
     3121 ggattccaaa cctatcgtac agatcttcta aaccacttat aaattttgca cctatttcag
     3181 gtgttttatt gcgtggatat tctattgtta cgccattaaa tggataccaa ttaggattag
     3241 gctctctttt tttctgtaca tctcccatag aatagttagc actttctggg acataatata
     3301 ttcttcgacc atcctctaat tcatctaata agatatttcg tgatcgtcta tcacttgcta
     3361 aatgtagacg ccacgtgcga taccatgcca tgctacctgc atctgcagaa aatccatcaa
     3421 ataatgcaac aattgtatct tctatcacaa tcctttcccc atctacttca tattctaatc
     3481 taaaatcaaa ctcccctcgg gtaatttctg gtcttggtgg cttaggaccc aatattattc
     3541 tacttaaaac cgcattcgtt cccagatgaa acacagctat aacaacaata atgattaata
     3601 ttttgtttct atttgataaa tttctcatta ttcgcacatc acctctacat tctcacatat
     3661 tagttcgtaa tactccttta atatcaaaat atctactggt cgtaataata ttcgctatga
     3721 attcctacta ctcgtataag aaaggccaaa tcatcagctt ttacttgctg atgatttggc
     3781 ttgtttctat ttatcgtaat tgttttctaa tcaccaaaat tgttaacaat cggttcagat
     3841 atttcccagt tgattaaggt aattccgtat tgtgtcaaaa cttcttgcgc ttctccgaaa
     3901 ttaagtacag ttcttatgta tccactttct cctaatataa agtctacctc tcttttaaaa
     3961 ccttcctcga gatttctaag ggctatatgt ggttttaatg acattgattg tacattttcc
     4021 cccatataat attgcgcact tcctcgacta aaatagattc ctagatcgtc cccaaaaggt
     4081 agatcttctc taccactagc taatctactt ctccatcttc gttctctttt catagaacct
     4141 gcgctgaact cacacccgtt aaactcagct atgacagtat cttcaattat gttaatctgt
     4201 ccatctagtt cgtattcaat cctaaatgga aattctccat aggtgatttc cggttcgggt
     4261 attttaggtg agattaatgc aaagaaagga aatattaagc ttggcaacat caaaaaaact
     4321 cctcctatga tcacaattcc cgatgtaatc caaaatataa tttgtacaat tctcattgat
     4381 ccattgtatt ccattttatt ccccctatta ctattcatat ccgcctatct atatcgatct
     4441 tccaatgcag agataattgc ttccatacct tgatttttta ctgagttacc tatgcgagat
     4501 atcattctcc actaaggtgc atcatattgt ctatctaaca tgattaattc tccaataggt
     4561 gtatactcct ccaaaaacaa catttaatgc atctccctcg acaacataag ctaccatgtt
     4621 tgctgtatat gtgtttgagt ctaattcata agcagataaa tttactgtcg ctggattaaa
     4681 tataatcgca ttcgtattta tagctactga atttgctgct tcagcaccac cttttgaatg
     4741 tccgatcatt gtgatttcaa tagtaggatt tacgtggaca gccctcaccg tttaccctga
     4801 ccattataga aatcgcttac ttttttgaac tacgcaacaa aggatacaat gctcctaact
     4861 tcactgtctc catttttgtc cactaaggaa tataaaaaat aggcattaat tcatttaatt
     4921 cggtcacaac acgttacctt ttaaatcatt atactgtatc tcatacatta ttatcaataa
     4981 actattataa aaatgcaaaa aatgctttat caatctattt tgtaattggt cataatatca
     5041 ttgaaatcat accctgtatc gatttttcct atgaatctcg tctatttgtg attgacgaaa
     5101 aagaggcaag acagacactg gaatggtaat tttacccact attttgctcc aactatttgt
     5161 aatcgtaccc acttttgaaa taccgagatg atcattttaa ttgatatgct gattcccaag
     5221 caaaacccca agaaattaaa tgctctatcg catctagctt cttgggtttt gcttatataa
     5281 attccaacat ttaaattttt tatgctgttc ggtctcagaa atgccgtttt tgcttatatc
     5341 acaatatcaa gatgtcaaat tattcgtgtt gccgatgact ttgattaatt tttttcaaat
     5401 tgtttattaa gactaatgct gtctttttgc agataccaat gttcgaactt tttaacatta
     5461 tcatatgatc ctgcaagttt taacttctta ccattagatg ttatcaaagt tataattaac
     5521 cttgcctttt tcccattagg atcttttatt ttatgaattt tctgcaggta atctctccta
     5581 atggcagcta tttttacctc atcagtgctg ataaaccatt gatcatccac atacatactg
     5641 cctgttatgt tgtgtttttc catttcttcc gagaacgaaa agtttaaggc tcgttcttac
     5701 ttccaaagtt gaatgagtcc atggattgga cttagtgtga gaattgagaa caacaataag
     5761 aatccatatc ctcccgcaaa catccctaag gctatactgt ctctccatcc ataagtagga
     5821 ttagaagaga aaagagtcac cgtttgtaat aatatgaaaa ggaataatcc agcacaaaca
     5881 ccaaagataa caactccaaa cattgaattt ttatacaatg accatttatt tataggcttt
     5941 tctttcattt tacttccccc tactgttctg ttcacacgga atgaattcaa agttcataag
     6001 ccctcctctt tgaacatatg aatacgactt gctaactata ctttaaaaaa gcttgtttta
     6061 agcggttata ggcgagttgt aatctctatt catctcatta gcaaataact tgccgaaaag
     6121 tctactgaaa ttaaagcaga tagtggaaga attatttagc caggatagac actatttgtg
     6181 aaacacatgt ctttactttt gaaattctat gatgaaaagc cttggaatac agtagctctt
     6241 aattggtaat actttttcca ttataaataa cctcttttca ttttatattt atcccctcta
     6301 aaaataatat ctaatctaat attttaaact tatatttccc tcgctgtgct aatagagcat
     6361 tactaaataa atagaaacta gaaaaaagca ttacgcctgc cagaataaac caattatttg
     6421 t
//
LOCUS       DADVXN010000128         6309 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.132, whole genome shotgun sequence.
ACCESSION   DADVXN010000128 DADVXN010000000
VERSION     DADVXN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.132"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(75..1553)
                     /locus_tag="KB942_002513"
     CDS             complement(75..1553)
                     /locus_tag="KB942_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852896.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1851..2786)
                     /gene="murB"
                     /locus_tag="KB942_002514"
     CDS             complement(1851..2786)
                     /gene="murB"
                     /locus_tag="KB942_002514"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBB6852897.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2859..3779)
                     /locus_tag="KB942_002515"
     CDS             complement(2859..3779)
                     /locus_tag="KB942_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6852898.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3892..4644
                     /gene="xth"
                     /locus_tag="KB942_002516"
     CDS             3892..4644
                     /gene="xth"
                     /locus_tag="KB942_002516"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBB6852899.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5508..6041
                     /locus_tag="KB942_002517"
     CDS             5508..6041
                     /locus_tag="KB942_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBB6852900.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1903 a         1204 c         1049 g         2153 t
ORIGIN      
        1 tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg agacaaagct
       61 gccatccata aatactagct gattttttca gttaaatgtc tatttttttc tctgatgatg
      121 gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat tatctgttct
      181 ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga agtgattcga
      241 ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat gtaatcggta
      301 taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa atatgatacc
      361 ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat gaaacgttca
      421 tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc tgtactgaat
      481 attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct agtcatctca
      541 aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag ttcttttcgt
      601 atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc aatcaattcc
      661 actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc aagtccaaat
      721 gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata agaaatctca
      781 tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc cttgatgatc
      841 ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc gattggttga
      901 ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat tcgttttgtt
      961 gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt tgcttcattc
     1021 atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata taccccaaat
     1081 aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa ctttatttga
     1141 tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga tatgcttaaa
     1201 aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc cgttaacagt
     1261 cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc cgtcaaattc
     1321 agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt tgttttgaat
     1381 tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt agacaaacct
     1441 aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc taaatatcta
     1501 gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt catatcctac
     1561 acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca tcttttcacc
     1621 tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca ttttttatct
     1681 tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct atattttttg
     1741 ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca caaaaaagag
     1801 caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta ttaattggct
     1861 aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac ttctgtttct
     1921 aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa ttcaatataa
     1981 tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc agagacttgt
     2041 gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt
     2101 cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg ttttgattga
     2161 cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc tgtttgcaaa
     2221 ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg acgataagaa
     2281 aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa aagatccact
     2341 tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata aaccgctcca
     2401 ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc tgccaaagct
     2461 gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca
     2521 tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc atcttgaacg
     2581 atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg gcaataatcg
     2641 atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc tggtccacct
     2701 gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat cgtggctaag
     2761 ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca aacgtcctca
     2821 ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg acggtagtga
     2881 tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg tttaagcaat
     2941 tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat tggctcaaac
     3001 ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt tccatcagca
     3061 tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt acttgttact
     3121 tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc cgtttgattt
     3181 tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct tatctggtat
     3241 tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag atgtaaaaac
     3301 aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg
     3361 atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa gcgccggttg
     3421 aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc acgaacttca
     3481 tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc tagacatttt
     3541 ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc gattttttct
     3601 tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt ttgaattctt
     3661 tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca
     3721 taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc aatcttcatc
     3781 tactttcctc ctggatcatt tagttacttt catcttacac catttttttc taaaatgagg
     3841 tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt attgaaattt
     3901 atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt agaagttttt
     3961 catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc aggacaaatt
     4021 gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa aggctattcc
     4081 ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat gggtatcgat
     4141 atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt tcttgtaacc
     4201 tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt agaatgggaa
     4261 gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg
     4321 gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat
     4381 gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa cgggtttatc
     4441 gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg gaactaccga
     4501 ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac gagcaaagat
     4561 ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag tgatcattgt
     4621 ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta
     4681 agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata ataccgagaa
     4741 actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat
     4801 aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct tattaccgca
     4861 gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac tgcatcaagt
     4921 aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg gcttattacc
     4981 gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg cactgcatca
     5041 agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt tggacttatt
     5101 accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca gcgtcctgac
     5161 ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt ttttgagcta
     5221 ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac
     5281 tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat aaatttgact
     5341 taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga gttcaaaaac
     5401 tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa tttatttgct
     5461 tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg aattttgttg
     5521 ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt gcgctagtca
     5581 tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct gagactcctt
     5641 ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc aatgcgccga
     5701 aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt ttagtcgttg
     5761 cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat tatcatcttg
     5821 aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat
     5881 ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta gatcatcacc
     5941 atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat
     6001 tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact tatgaaacac
     6061 acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata
     6121 gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg agaacaccgt
     6181 ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
     6241 atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag cttttggaca
     6301 ccgtttacc
//
LOCUS       DADVXN010000129         6184 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.133, whole genome shotgun sequence.
ACCESSION   DADVXN010000129 DADVXN010000000
VERSION     DADVXN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.133"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            127..972
                     /locus_tag="KB942_002518"
     CDS             127..972
                     /locus_tag="KB942_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6852901.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1253..2473
                     /locus_tag="KB942_002519"
     CDS             1253..2473
                     /locus_tag="KB942_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBB6852902.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2470..5190
                     /locus_tag="KB942_002520"
     CDS             2470..5190
                     /locus_tag="KB942_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6852903.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5174..6118
                     /locus_tag="KB942_002521"
     CDS             5174..6118
                     /locus_tag="KB942_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6852904.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2306 a          968 c         1203 g         1707 t
ORIGIN      
        1 ataaatgtcc tttcctattc ttaaaatgat gtttgtactt acttaaatta ggtataatta
       61 gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata
      121 ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct tttgaattct
      181 gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt
      241 ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt
      301 ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata
      361 tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt
      421 ccaaccaaaa tatatacaaa tctcggaagt tatgaatctc agaattataa aaataaaatg
      481 aaaaaaattt ttcacgaggt agggagtgaa ttttctatca aagagctaaa ctatgagctt
      541 gagtatgaaa aatctatttg ttcaatccct tataataata ttgaggaaat tatagaaaag
      601 gatgctatca gcatttacaa attttttatt tttattccta atcgtcaatt gaaagctaat
      661 atatttaacc atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat
      721 attgaaatcg tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc
      781 tacaatttaa agaaaccttt gagaatcgcg atcggagata gtttaaatga cttatctatg
      841 tttgaaagtg cagatattag ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa
      901 gctgattatg tcactgtttc aaatgataaa gacggagtat ctcaagcatt tgaaagaatt
      961 cttaccatat aaactaatta aggtagtata gaacatagaa ttctaaatac ggaaaaatgg
     1021 agcataaatt ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa tataacatag
     1081 atatttagga ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa cttatttttt
     1141 gaagtcggcc atttatcgta atttctttgc ataatacgaa caaattttcg gttattgatg
     1201 aaaaaaaaca ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa
     1261 atttatccat gcggcagatt tacatatgga tcggtcattt gaaggactga caactcttga
     1321 taaaactgta caggaaaaac tattgaagac gaatctgacc gttttatcaa atatcattga
     1381 acaggcaata ataaataacg ttgattttgt tttacttgca ggagataatt ttcatcaaaa
     1441 tcgtccttca ttaaaaatac agaaacattt ttcagaacag atgaaacggt tagaaaagaa
     1501 ccatatacct gtatttataa tattcggaaa tcatgatttc tatcaaaaag agcggtattg
     1561 gttctccttt cctaaaaatg tccatttatt tacatccgaa acggtagaga caaaaaaaat
     1621 aacgataaaa agtggagaaa ctgtctctct cagtggattt agttatcgtc aaccgtggat
     1681 tcaaaaagac aaagtgatgg aatttccttc tagagaactc acagattacc atattgggtt
     1741 gtaccacggg gaacctggcg tctctcaaaa aggaaactat gctcctttcc aaccctcaaa
     1801 gatgcaagaa aagggatacg attattgggc tttagggcat atccatgtgc caacggtctt
     1861 aaatgaaaag caaacaattg tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca
     1921 agcttctaca tctattctac tagttgaatt aagtaaagga agttgccaaa tacatccgat
     1981 taaagtagca gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag caagaacgac
     2041 aaaagaggtc attgttcaca ttcgtcagca gctttcaaaa gtagaggatg gatttagttt
     2101 aattgagata aaattaaaag attatgacca tctcaataca gatgttttgg aacggataca
     2161 atcaggagag ctattagact atttgttaga ggaattctca gataggataa atgatttctt
     2221 tatttggcgt atttctctca tagagaatac tttttatacg aagaccccac ttgctgcttc
     2281 tgctaaattg atggaacagt tatttcaata ctatcagaca cctcaagatt tccaacaaat
     2341 tttaaatgaa gtatatagtc atcaagaagc ggctagaata ttgagcgaac tgccggaata
     2401 tcaagaagaa acacttgaaa aagccaagca gctgcttaat caagattttt tatttgagga
     2461 ggatcaggaa tgaagatttt aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa
     2521 agttttgact tttattcagg caatcagctg atctatgggg gaaacgaagc aggaaaatca
     2581 acgatttatc aatttattca agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa
     2641 aaggattata caccaaaaga cggctcagcc tatggcggca aaatatggct taaacatcct
     2701 gtatatggtg aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg
     2761 tggctggggg atcaagtggg aagtgatgag ttactagaaa aactgatcgc acctttgacg
     2821 aaagagttat tccagcaggt atttactttc cagcaagagc agttgatgga actagatcat
     2881 ttgagagaaa aagagctaca tgatgcgctg atttctttag gcatcacggg cagttctcaa
     2941 gtattccaaa agcggcaaga atattatcag aaagcacaac aattattcaa gaaaaaaggc
     3001 caaaaattac taatcaatca aaaattaaat gaatggaaag agctgcaaga aaaaattcat
     3061 caaaaacaag aacaggaagc ttattttggc aagttgatcc aacaagaaca ggaatacgag
     3121 tcgcaacagc agcagttatt ggcacaatta aaagaggcga acaaacaatt ttcattagcc
     3181 agacagcaag aacttaattt ttctttatat gaagaatggc aatctctcaa aaaaaacagc
     3241 actacaggtc ttcctgatga gcaacttctt ttagatctgg agtctttctc caaacggtat
     3301 cagcaaacaa atgagcgttt ggaagaatta gaagcaacac tggaaaagca tagcgggatg
     3361 gatcagcagt cagccagata ttatttttat ttagagcagg agcaggaact aaaagatctc
     3421 cagcaacttc aatttacagc ggaacaatta aacgaagaat ggcaaagatt acaaattgag
     3481 actagagaaa caaattcaga attgaagatt ttggaacaaa ggtggcattg gcagaaaaat
     3541 ccgttaccgg aagaattttt atatgaagaa gaatggaagc atcttctggc tgaagaaaga
     3601 aggattgctg atctagaaag ccagatttct gtgcgtctcc aaatagctaa agaacagcaa
     3661 gagatgattg aagcagaagt gaatgacttg gaagagacct atccagcatt attacaaaat
     3721 cagtcagaga tagcaccagt ctcttcatct aaaccgtata tgctgatggc tggcggagca
     3781 ataatcattg caggtatatt tgtaccaatg cccttgaaag tgatcctttt actaattggt
     3841 gcaggcatga ttggctattt cttctacaaa aggcaaccaa aaattaaaac taaaaatgat
     3901 ttctctgaga taaaaggaat gtggcaagaa aaactaggac aacttgatct agccttagct
     3961 caattagctg aagcagaaga aaaacaaaag agaactggag aagaaaaaca gattcttcag
     4021 caagaagtat atcgttttgc ggatcatcgg cacttaggaa agatggatac gctcaaactc
     4081 atgaaagaat atggacagga agtcatagat tatcagaaat tatatggcgt atttgagcgg
     4141 aaacataaaa gagaacagga aatacaagaa aaactacgac aaatcgacca aaaatttgag
     4201 tttgttaaag aatggttacc gatacaagat aagacaattt cagaaaaaat ggtattgctt
     4261 tcccgtttta tacaagagat ggaacagatg aaatttgcca gaagctatca gcaaaatact
     4321 ttattgaagc aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat acaagaatac
     4381 agcgaacaat tgactcaagc aggcttatct tatccttcgg aagtacctgt ctttttacaa
     4441 aaagccaaac aagccgaaca gcagatgaaa agatacaatg aattatcgga aatgcttact
     4501 ccactatttc ctgaagagct gacaaaagag aaattatctg taagactgcg ccaactgaaa
     4561 gaaaagcaat cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc
     4621 caacttcaag tagaagagtt gcaaagagac ggcacattgg acgaacttta tcaacaagct
     4681 agccggcaaa aagcaaaatt acaggaatta ctggaggaat ggggagcact tgaaatcgcc
     4741 gggacattct taggtgatct ttcaacagag atgagtgaaa gacaattacc ccagttgtta
     4801 agagcagcag gtgattattt aggtatttta acaaaaggaa attatcagaa gatattactg
     4861 aaaaatgata cactgacttt aacagacgga tatacagaat ttcccatcta tgagttatct
     4921 acaggtacga aagatcagct gattatggcg atgagatttg cttacctctc attagaagct
     4981 aaccgttcga tatgcccgat catcattgat gatggctggc ttcattatga tcatcagcga
     5041 aagtatcaat tagctgaact tttagctaaa tttggagaaa agtatcaaat aatttgtttt
     5101 tcttctgata aagaaatggt aagctattac catgagatga accaaatcgt aaaacaatta
     5161 gaaggagcaa ataatgaaaa aaattcgtga tttagtaatc gatgagcagt ttgaacaatt
     5221 tgtcctgatc aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt
     5281 tactttccaa gatacttcag gtactatcga tggtaaatac tgggatgctt cggaggagga
     5341 aatcaataaa tttacagccg gcacagtcgt ttttctaaac ggtaaaagag aggtttatca
     5401 aggaaaccct caagtaaaga ttcttcatat gcgcttaaca agggtagatg agccaggaga
     5461 gcctagttta tacatggaaa gagccccttt gaagaaagaa gaaatggaag aagaaatcaa
     5521 tcaattgttg tttgagatca ccaatgcgca ttggaatcgt attgttcgct atctacttaa
     5581 taaatatcaa aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc
     5641 cagtggatta gcttatcata ctttgacaat gcttcactta gggaaatcaa tcgccgatga
     5701 atatcctgag ctgaatcgag cattgttgta tgctggtatc attttgcatg atttaggaaa
     5761 ggtgattgaa ctttccggtc ctatgtcgac agaatatacg cttgcaggaa atttgatcgg
     5821 tcatctggta ttgattgacg aagaaataac taaagcctgt cttgcgttga aaatcagcga
     5881 agctgacgaa gatgttctcg tattacgtca tatggtttta gcacaccatg gcttattaga
     5941 atatggctca cctgtacgtc caagaataat ggaagcagaa atattacatc aaatcgacaa
     6001 tatcgatgct tcgatgcaga tgatgttgac ttcgattcgt caaacagaac caggaaaata
     6061 caccgatcgg attttcggta tggacaaccg aagtttctat gtaccaaaag atttatagca
     6121 aaaatttatt tttttcattc aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt
     6181 tttc
//
LOCUS       DADVXN010000130         6173 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.134, whole genome shotgun sequence.
ACCESSION   DADVXN010000130 DADVXN010000000
VERSION     DADVXN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.134"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            725..811
                     /locus_tag="KB942_002522"
     CDS             725..811
                     /locus_tag="KB942_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6852905.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="KB942_002523"
     CDS             complement(932..1621)
                     /locus_tag="KB942_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852906.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="KB942_002524"
     CDS             complement(1866..2780)
                     /locus_tag="KB942_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6852907.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="KB942_002525"
     CDS             complement(2762..3142)
                     /locus_tag="KB942_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBB6852908.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="KB942_002526"
     CDS             complement(3592..3879)
                     /locus_tag="KB942_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6852909.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="KB942_002527"
     CDS             complement(3899..4123)
                     /locus_tag="KB942_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBB6852910.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="KB942_002528"
     CDS             complement(4578..4748)
                     /locus_tag="KB942_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852911.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="KB942_002529"
     CDS             complement(4763..5296)
                     /locus_tag="KB942_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6852912.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="KB942_002530"
     CDS             complement(5317..6159)
                     /locus_tag="KB942_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB6852913.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DADVXN010000131         6148 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.135, whole genome shotgun sequence.
ACCESSION   DADVXN010000131 DADVXN010000000
VERSION     DADVXN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.135"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..124)
                     /locus_tag="KB942_002531"
     CDS             complement(<1..124)
                     /locus_tag="KB942_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HBB6852914.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKV"
     gene            complement(178..1995)
                     /locus_tag="KB942_002532"
     CDS             complement(178..1995)
                     /locus_tag="KB942_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6852915.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2009..2773)
                     /locus_tag="KB942_002533"
     CDS             complement(2009..2773)
                     /locus_tag="KB942_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBB6852916.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2801..3499)
                     /locus_tag="KB942_002534"
     CDS             complement(2801..3499)
                     /locus_tag="KB942_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBB6852917.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3511..4290)
                     /locus_tag="KB942_002535"
     CDS             complement(3511..4290)
                     /locus_tag="KB942_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBB6852918.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4306..5250)
                     /locus_tag="KB942_002536"
     CDS             complement(4306..5250)
                     /locus_tag="KB942_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6852919.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5296..6075)
                     /locus_tag="KB942_002537"
     CDS             complement(5296..6075)
                     /locus_tag="KB942_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852920.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1593 a         1289 c         1121 g         2145 t
ORIGIN      
        1 aaactttagg taatactatt ttttcgtcac acatatatag tgcgggtgct agttcagagc
       61 aatctgctcc aatgattttt ccttccattt cattattgtt taatgctgtt ttaaatgcat
      121 tcactagttc acaacgacgt ccacaactta atattaaaat attcaatttt taatctccta
      181 tctattcgaa gcatttgcaa aagtgacaat gtctttagcc aattgttcat cgtcttgcgg
      241 caggctcttg acaaaatcca tcactttttg tattgagtaa cctttgatgt ttcctacaaa
      301 gattttctca taaactgctt catcatttct ttccttatcc aacaatagtt cttcatatag
      361 tttttcccct ggacggatac ctgtttcaat gatttcgatt tcgtcttcgc tatacccgct
      421 caatcggatc atgtttttcg ctaggtctac aatcttcact ggttcactca tatctagaat
      481 aaaaatttcg ccaccttttg ctaatgcgcc agattgaata actaaacgac tagcttcagg
      541 aatcgtcata aagtatcgag tcatacgaaa gtctgttaca gtgattggac caccttgagc
      601 aatttgttct ctgaatactg gaatcacact accgcgagaa ccaaggacat tcccaaatct
      661 gacagctgag aatttcgtac agccttcttc attcaatcca gtcacaatca tttcagcgat
      721 tcgttttgtt gaacccatga catttggagg attgttagct ttatctgtcg ataccatgac
      781 gaagtttttc acttttgctg ctttagctgc ttctgctaca ttttttgtac cgaaaatatt
      841 gtttttgact gcttcttttg gattatattc catcagtggt acatgtttat gagcagctgc
      901 atggtagacg atatctggat gatattgttc catgatctca ttgattttct cgcgatcttg
      961 gatatctgcg atgatcggta cgatttctgt aggacaattt tgatgatgcg tgcgtaactc
     1021 acggtcaatc aagtagatcg agttctcccc atgtcctaaa agcaatagct ttgcaggatt
     1081 gaattgaatg atttgccgac agatttctga accgatcgat cctcctgcgc cggtgacaag
     1141 aatgactttg tctgtgatct gatctttgat ggattcaata tccagcttta cctcatctct
     1201 tcccagtaaa tccactacgt caattgtttt taattttgat acgctaattt tccctgaagc
     1261 aagttcttct atagaaggca tcgtgttgac tttcacacga gcggattcga ctaactcgaa
     1321 gattcgtcgt aatttttttc ttgataagga agggatagcg atcgttacca tttgaatgtc
     1381 atatttttca atcaattccg gaatatcttt taatgcacct aatacttttt ttcctgaaag
     1441 atacgtattt cttttatttg gatcatcatc tacaaacccg acaacgtgga tatcttgagc
     1501 cgttttggat ccaagaaagc tgttgtataa aatacgcccg ccttcgccag caccaacaat
     1561 caatgtattt ttagcagaat ctgctgatac gtatctcata ttttttgttt cgatgtagat
     1621 acgccagatc aatcggctac cgatgatcaa taagagcgaa agcagataag caaagataac
     1681 taaccgtaaa ctgtatcgcc gattaatgaa cagcagtatg aggatacttg aggcagcact
     1741 tgcagatagt gaactaaaaa ttgcaatgat ctctcttaaa tttgtatagc gattgatccg
     1801 agtgaaaact ttgaataaac taccataaaa taaataaaaa ccaatcgaca aaccgataga
     1861 gattaaaata agatccattg gtatagatac aaacggcttc atgaatttag ctgctgcaat
     1921 atttgcagca attaataaca agctgtccac aaaaaccaat gtggcaattt ttcttcttct
     1981 tgttaatata aacacttttt aattccccct aaaataaacc aaacttcttc ttttttacct
     2041 cttcatagtc tggataactg atttcatccc cgttgatcag atcctttgca acttgcttca
     2101 tctgtactac tttttcctta ccaaagtcct tggcaatctg ctcatagcac tcttttaagt
     2161 aaaaggtgcg cttagagact ccgtgtgcat cagaagcagt catctgcacg agattatgtt
     2221 tgaccattgt tttagctgtt ttctgaatat ctttgccaaa taccccaaca taacttggcg
     2281 cagtaagttg ggccaaacac cccatatcta ggaacggaag caaatgattc gggtcttcgc
     2341 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tctaacaact
     2401 caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     2461 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg atgagcgttc
     2521 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     2581 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     2641 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     2701 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     2761 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     2821 cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt gcttgtacca
     2881 tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt tcacgtacga
     2941 ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gctactactg
     3001 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg atctgatcca
     3061 ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     3121 acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat gaactaagtg
     3181 ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     3241 aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact gcaatgtttg
     3301 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     3361 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     3421 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     3481 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     3541 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     3601 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgc
     3661 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     3721 acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt tattatttgg
     3781 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     3841 cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagt
     3901 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     3961 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     4021 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     4081 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     4141 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     4201 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     4261 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
     4321 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc tgactttttg
     4381 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct tgatcgtacc
     4441 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     4501 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     4561 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     4621 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
     4681 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     4741 accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga catagtgatc
     4801 gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc cgccgaatgc
     4861 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
     4921 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
     4981 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca tcaatagaac
     5041 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     5101 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     5161 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     5221 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     5281 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     5341 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     5401 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     5461 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctagata
     5521 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     5581 agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag cttgctgcca
     5641 gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca gttttgattc
     5701 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     5761 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag aggatagctt
     5821 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat ttttttcttt
     5881 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     5941 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     6001 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     6061 gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta agatataatt
     6121 cacatacata aaaatgacag ctttttat
//
LOCUS       DADVXN010000132         5993 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.136, whole genome shotgun sequence.
ACCESSION   DADVXN010000132 DADVXN010000000
VERSION     DADVXN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.136"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(32..334)
                     /locus_tag="KB942_002538"
     CDS             complement(32..334)
                     /locus_tag="KB942_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852921.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(554..920)
                     /gene="ssrA"
                     /locus_tag="KB942_002539"
     tmRNA           complement(554..920)
                     /gene="ssrA"
                     /locus_tag="KB942_002539"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1091..1864)
                     /locus_tag="KB942_002540"
     CDS             complement(1091..1864)
                     /locus_tag="KB942_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6852922.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2008..2358
                     /locus_tag="KB942_002541"
     CDS             2008..2358
                     /locus_tag="KB942_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBB6852923.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2339..3055
                     /locus_tag="KB942_002542"
     CDS             2339..3055
                     /locus_tag="KB942_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6852924.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3055..4503
                     /locus_tag="KB942_002543"
     CDS             3055..4503
                     /locus_tag="KB942_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6852925.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4574..5083)
                     /locus_tag="KB942_002544"
     CDS             complement(4574..5083)
                     /locus_tag="KB942_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852926.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5073..5798)
                     /locus_tag="KB942_002545"
     CDS             complement(5073..5798)
                     /locus_tag="KB942_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852927.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2039 a         1043 c         1166 g         1745 t
ORIGIN      
        1 cgttccgggc taccttttta ggtagcctac tttaatcttt gtatctttct ggatcaacga
       61 aagtatactt tatatagtca taacgccgat gatcgcttcg agcgtctagc acatcagtca
      121 cgatatcaaa caaaaaatat acgtctttct tcattctagt tttcgcagca gggattttaa
      181 agtagttctt attagaatag tagagattaa ttaataagct atcttcgatt gctaaaaaga
      241 aaacttctga atcccacacc ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt
      301 taaaccataa ttgtgttttt ccttccatca acataatgtt cacctcaaaa agagtatacg
      361 aactaatgtt ctttttgtaa agtaggaata tcattgataa tatggatgga acatatatat
      421 taaaaagtgt tctttttgaa tgctattttg aatgccattt tcattcattt aacgaaaatc
      481 tgcgaaaata aaaaatgctc aaacccttat aaaataagga tttaagcatc tagcgaaatc
      541 tgctgaaatc tcacaatgga gacggcggga gtcgaacccg cgtccaaaca catcgccact
      601 taaaaatcta cgttcatagt caactcattt aaggattcac tttacagctt gccgagtgac
      661 aggcattctg tatcgctagt ctgatgttct ctttttcaac ttacagacgg aaagttgaaa
      721 cgtatcccac taaagttagg acccttactc gagcacatgg gcgatgccga gaggatctac
      781 gctaactgtt tttaggcagc taaagcgtaa gagttgtttt cgtttttagc agttatattt
      841 aactgtaacg tttttacgta gacgtaacct acgaaacgca attcaagctc gactgtgcct
      901 gtcgaatccg taacgtcccc gttagctaac aagggttacg tgtatttttc atacaaaatt
      961 agtatagcat atattgggat aaattcaata aaaaagcgaa tcatatccca ttatttatat
     1021 aataactgcc cctttcttaa gaaaaaataa gcgattttct taagaaaaga ggtagcttca
     1081 aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg gacaattccg ccttctgtat
     1141 ttttctttgt gataaaggta gaaagttctt ttaatccgtc ataggcgttg cccatagcga
     1201 ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg accatcgcca aatccaaaag
     1261 taggaacttt ttccagctga agttcttttt gcagacgttt gacacctgcc ccttttgaga
     1321 ttcctttctt cactgtatcg atcgagtaag gaccattccg ataaaaagtt agctcaggaa
     1381 aatgttcgtg atagtattcg tcacctgatt gagataagat cagcaacata tttactgtgt
     1441 tttctttaaa gcctaaagga tcaatttccg gaacatctga atgaatgaag tcgtaagcat
     1501 tgatcattgt ttggctatga ccggtacacc agattttctg atcgttatag taagaaagtt
     1561 catgaccgtt caactgtaca tgatcataca ttttttgaca ttcttctgaa gaaaatgttt
     1621 gagaatagat ttcttcgccg tctactcgaa tgaaagaacc attcatggtg accgcagaag
     1681 taatgcccgc atcttccatg atagagtgga tttctgcttc tgtacgtcct gtagcgatga
     1741 cggggagaac attgttttct tttaatgcgt tcatagcttt tgccacttcg ggtgtgattt
     1801 ttgatttcgc atctagtaat gttccatcaa gatcaaagaa agtgattgca cgataatttg
     1861 acacaaataa aactcctttt ttattggaat aagtttatct taacaaagaa tgaacaaaag
     1921 acaaaccttt cgtgaagaat tcctgaacat attggttcag tcaacaattt agagtatact
     1981 ataagaagag aatttaggag ggaaagaatg atttttttca gtattggtat cttattctta
     2041 ataattggca gtctttttcg gattctgccc tcaaaaggaa atctgccttt ttatgggtat
     2101 cattcgcctc ttgccgcaaa aacagacgct cattggcggc tggctcaaaa gacaagcgga
     2161 aactggtttt ttcttatggg cttactgatg gcattgatcg gctattattt aaagacatct
     2221 gggcatacga attattttct gattgaaatg cttctgctag tatttccaat tatgcctatt
     2281 tttattatga cagaaaagaa attacagaaa tatgacctgg aaactggagg gaacgataat
     2341 gaatatttta atgattgaag ataatcaatc tgtttctgaa atgatgcaga tgtttttctt
     2401 aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa gaagggctcg atgccttttt
     2461 agaaaaccca caacattggg atatgattac actagacctg aatttgccca cactggacgg
     2521 tatgctagta ggaagagaga tccgcaaagt ttcaagtact gtgccgatta tcatgctgac
     2581 agctagagat tctgaaagcg atcaggtgat cggcttagag atgggggcag atgattatgt
     2641 cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg aaagctttgc atcgccgtag
     2701 tgagttggca gaagcaagag aagaagcagt agtttcagat gatcattttg atatcgagac
     2761 tagtcatttc aagatgaata cgaaaacacg agaagcgtat ctggacaacc ggccaattga
     2821 aggactgacg cctaaagagt tcgatctatt gtttacttta gcaaaaaaac cacgtcaagt
     2881 attctctcgg gagcagttgc ttgaattagt ctgggattat cagtactttg gtgatgaacg
     2941 gacagtggac gcccatatca agaaattacg acaaaaaatc gaaaaggtcg gaccacaagt
     3001 catccaaact gtttggggag tcggctacaa attcgatgat tctggtgttg cctaatgcgt
     3061 tatttgtatc agcaactctt agctttctgc ggaatggtcg cgttgatctt attgatcgtt
     3121 ggagtttctt ttacacaact gacaaaacaa acgattgaag aaaacaatta ccagcaactg
     3181 tttggatatg ctgaatcagt tgaaaagaca acgcaaacct atgccgatac gctcccgcag
     3241 tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga cagagcaagt actgacagaa
     3301 caaaatgtga actttatatt tatcgataaa aacgaacgtg tcatttatcc gactaataca
     3361 gctttactga attttacctt aacagctgat caatggaaaa atctgaggaa tggacgtcaa
     3421 gaaaaattca catcgaataa aaatatttta ggacaagcac aggcgacctc ttacgcactt
     3481 gtgccattta atttgaatca tgaattctat ggagcacttg tagttagtca acctgcgcga
     3541 aatatcgata acagcgttcg tccaattaca ctgaacctgt tcaaaggatt tattttttcc
     3601 agtatcgttg cagttattgc cagctatgct tttgcagcat tccaagttaa gcgtatcaat
     3661 cggttaagga atgcgacgaa agaagtgacg aatggtaatt ttgatgtaca gctccccgta
     3721 catgacaaag atgaatttga tgagttagca gatgatttca ataaaatgac gaattcattg
     3781 aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc gaagacaatt tatggcagat
     3841 gcttctcacg agatgagaac acctcttacg acaatcaacg gacttctgga agggttagaa
     3901 tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta aattgatgaa aaatgaaaca
     3961 gagcgtctga tccgtttggt gaatgaaaac ttggattatg aaaaaatccg tacgaatcaa
     4021 atcagtatgg tcattaaaaa attcaatgga acagaaacat tgaaaaatat tgttgcacag
     4081 ttagaagcta aagcagaagc agcaggagat acattgacat tgaaagccgt tgatgatatt
     4141 gatgtttacg ctgattatga ccgatttgtc caaatcatgg tcaatattat tcaaaacgcg
     4201 atacagttta ctgaaaacgg ccaaatcacg attactttag aaaaaggcta tcttgaaacg
     4261 atcatcacga ttgaagatac gggaattgga atgtctgaac aacaaatgaa aagtatctgg
     4321 gatcgttatt ataaagtaga tccttcaaga aaaaatacaa aatatggtga atcaggatta
     4381 ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag gaaaattaga agtagaaagc
     4441 gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg acgtagaaat caaaaaagag
     4501 taaaaaacaa gcggtataaa agatcatgaa tcgatcttct ataccgcttg ttttttacta
     4561 taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag
     4621 tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca
     4681 agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa
     4741 tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg
     4801 tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg
     4861 ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat
     4921 tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa
     4981 caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa
     5041 taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta
     5101 ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca
     5161 ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt
     5221 tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa
     5281 gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt
     5341 tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa
     5401 caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa
     5461 agaccagtca ggtgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag
     5521 aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat
     5581 cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa
     5641 agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct
     5701 atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa
     5761 taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt
     5821 atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca
     5881 gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga
     5941 tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat ttt
//
LOCUS       DADVXN010000133         5991 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.137, whole genome shotgun sequence.
ACCESSION   DADVXN010000133 DADVXN010000000
VERSION     DADVXN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.137"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..450)
                     /locus_tag="KB942_002546"
     CDS             complement(<1..450)
                     /locus_tag="KB942_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6852928.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="KB942_002547"
     CDS             674..1465
                     /locus_tag="KB942_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6852929.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="KB942_002548"
     CDS             1431..2384
                     /locus_tag="KB942_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBB6852930.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="KB942_002549"
     CDS             complement(2782..3756)
                     /locus_tag="KB942_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6852931.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="KB942_002550"
     CDS             3871..4305
                     /locus_tag="KB942_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6852932.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="KB942_002551"
     CDS             complement(4430..5452)
                     /locus_tag="KB942_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBB6852933.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5991
                     /locus_tag="KB942_002552"
     CDS             5844..>5991
                     /locus_tag="KB942_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBB6852934.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIK"
BASE COUNT         1887 a         1129 c         1031 g         1944 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa a
//
LOCUS       DADVXN010000134         5779 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.138, whole genome shotgun sequence.
ACCESSION   DADVXN010000134 DADVXN010000000
VERSION     DADVXN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.138"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..813
                     /locus_tag="KB942_002553"
     CDS             <1..813
                     /locus_tag="KB942_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6852935.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="KB942_002554"
     CDS             807..1229
                     /locus_tag="KB942_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852936.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="KB942_002555"
     CDS             2363..3331
                     /locus_tag="KB942_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB6852937.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="KB942_002556"
     CDS             3341..3829
                     /locus_tag="KB942_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6852938.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="KB942_002557"
     CDS             4125..4406
                     /locus_tag="KB942_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852939.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="KB942_002558"
     CDS             4408..4824
                     /locus_tag="KB942_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852940.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="KB942_002559"
     CDS             5438..>5779
                     /locus_tag="KB942_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6852941.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          980 c          948 g         1697 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 agaggggcta cttggcatag tgtacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DADVXN010000135         5696 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.139, whole genome shotgun sequence.
ACCESSION   DADVXN010000135 DADVXN010000000
VERSION     DADVXN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.139"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..434)
                     /locus_tag="KB942_002560"
     CDS             complement(<1..434)
                     /locus_tag="KB942_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBB6852942.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDA"
     gene            complement(447..671)
                     /locus_tag="KB942_002561"
     CDS             complement(447..671)
                     /locus_tag="KB942_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852943.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(775..2685)
                     /gene="ltrA"
                     /locus_tag="KB942_002562"
     CDS             complement(775..2685)
                     /gene="ltrA"
                     /locus_tag="KB942_002562"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6852944.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(3431..3568)
                     /locus_tag="KB942_002563"
     CDS             complement(3431..3568)
                     /locus_tag="KB942_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBB6852945.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(3565..4749)
                     /locus_tag="KB942_002564"
     CDS             complement(3565..4749)
                     /locus_tag="KB942_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6852946.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(5008..5298)
                     /locus_tag="KB942_002565"
     CDS             complement(5008..5298)
                     /locus_tag="KB942_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852947.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(5288..>5696)
                     /locus_tag="KB942_002566"
     CDS             complement(5288..>5696)
                     /locus_tag="KB942_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6852948.1"
                     /translation="RVYEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFP
                     NSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVN
                     SDSQLYKQAGNSVTVPVIFDIARRLKELDVHGL"
BASE COUNT         1524 a         1285 c          903 g         1984 t
ORIGIN      
        1 cacgcatcaa aatcaatata cgtcttcaag tgttctggta cttctccaaa atcaccagct
       61 tctacaatca agaaatatgc tacatcactc atatcatcgc aatctggata atagataata
      121 tcatccacat gctctaccat ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa
      181 gcaacttcac caactggcgt tccttcaagt tcctccgcta agccacacaa ccgattaatt
      241 tcctcaattg gcgtatactc atcaatctca aagggaagtt cataatcatg aattgcatat
      301 tcctcgtatt cgtcatttaa cccaattcgt tctttcactt catcaaaatc gacaggtggg
      361 gtaaaccacg cccccaccaa ttcaccttcg ttatatttcc cgaggttcgc aatataaaca
      421 cgcatctgtt ccatcgtgct tcccccttac gcaccgacaa ttcggttaaa caaactgagt
      481 aagacatcct tcaccccaga agcattaaag accaatccga ccgcaatcaa agcaatcacc
      541 aaaaagccaa tcagtttgga aaactcccgc ttaaatccta aaaacaaacc aatgaccaca
      601 attgccagta agactaagga ttgggcattg gttaaaaacc attgatatat ggataggtaa
      661 gttcctgata ttataaatat ctcaaacttt tccccccatt cacacggagc gtgcgacttt
      721 caccgcactc cgcgttccat cgtgtttata ttgaaaagta tttatgtagt agacctatat
      781 caagtttaat tcttgattgc cagctaatac tctatattta tttagtctta tgagctggac
      841 tttatttagc ttcaatagag ctatatattt ttgtattgtt tcttctgttt tagcgtgtat
      901 tagacggtgt accgcctctt ttaccaaaat aagattttta taatcgtcat ttccaccagc
      961 tccagttggt acaatatgat gacaatgtat ttcattagct tgttgaaatt cttccagagt
     1021 tatagcacat tttccatgtt gagcagaaaa tagcgaaatt cgattgtctg cgtattctat
     1081 tgtgcgattt tccaaagata atttcattaa ctggtacatc agtaatttat caacacttag
     1141 attggtgtgg ataaattttc ttccctctag agtgtagcaa ttcgctaaat acacctttgc
     1201 cattggtttt ctgtgttgga tatatccaac agggaaaata ggttcgccct cgccagcaac
     1261 ataccgaagc attttagatt taccatatct tgttgcctcc tttgtcgtaa gttgtctgcc
     1321 tgtttttctc aatctgcccc ttttgtttgt acctaatctg ttagtgaaga tagccattac
     1381 agctctattc agtttcatac aatccagatt tacattcgtt gcaatctgat aataattttg
     1441 catacctaat accattgaat tataaagtct tgtttcttct aattccgttt tcccttttga
     1501 gggagaagca attttctttg cctgtgatac cagcttgtct ttttggtttt tgaattgttt
     1561 atcacttata tgtgatttaa caactaactt ctttcttctc ggtatcattt taatcttgaa
     1621 accaagaaag tcggagtatc tttttctaac atttacaatt cttgtttttt cttgtgatac
     1681 ttccaatttt aatcgttgtt caatccattg agtgattgct attttggctt tttcagcact
     1741 ttccttgtag cggcagaaaa ctctaaaatc atctgcataa cgaaccatat acatttcctt
     1801 taactttgtt ttcttcattt ctttgtaagc gttgctttta catgggctac cgcttttatt
     1861 gatatgaacc acatagtttt ttgtaacagg attttcctgc cattggcttt ctacccaatg
     1921 gtctaactca ttaagaacaa tgtttgctag tagtggggat attattcccc cttgcggagt
     1981 tcctttctct ggatatgtta ttgtaccgtt agtcattttg attggagctt ttaatatcct
     2041 ttttaaaatg aatatcaagt gcttgtcccg tattcccata gcccatatct gttttatcag
     2101 cttggaatgg ttcacgttgt caaagaaacc cttaatatca aattcaatca cataatgtag
     2161 ctggctgatt tgtaggcggt tataggttgc ttttattgca ttttcaactg acctattagg
     2221 tctaaaacca tagctgtttt cactaaattt agcttcgcag attggttcta atacttgttt
     2281 tatacattgt tgtataagcc tgtcccacat acaaggaata cctagaggtc tggttttacc
     2341 atttggtttt ggtatttctt ttcgcctcac tggcttaggt cgatacccat gtggactgcc
     2401 tgcaacaatg tatcttactt ttttgacaac tgtttctgct ggcaatttgc caatatcttt
     2461 aattattgta ccgtctgttc ctgctgtttt actgcctgca tttgctttga tatttcgata
     2521 tgccagtaat atattttctc tcgataggat aatatccata agatgagtga aaatatctcc
     2581 attcttactt ttggcatata aatcatcaaa ggtgttttgc atttcgtaat attcagcgtg
     2641 tcttaaatct tctacacaca atgtttcatt tttattttta ggcataaggc ttcactcctt
     2701 tacttgaaat tgagtgagct tttttatctt actcgtaatc cttattgaaa caacataata
     2761 cttttctaat atatacacga cttgagggca ttcctccatt ctcattacaa gaatttctta
     2821 ggttcgccct ctgcttttca gcattagttc aactgcttat tgttcttcgt cagtttattc
     2881 tcttgatgag aacccaatac ctttcctcgt tccgataatc ttatctatac atatatatcc
     2941 ttaggtcact agctataagc ctgctggctt gataccgcct gtaacgttat atggcatttc
     3001 ataacctata ttcttacttt gccacaccag ctacgatttc tcgtaccttt gcatttctac
     3061 aaagttcaaa tttagacccg tacattcgct gtttcgtcag ttccttatag aacattctaa
     3121 ccatagatat tttatagacc cccgacctgt tggatacgct acccacctct ggatagtttt
     3181 cgatagaaga tatatgttca tcaactatta cggtatcttt cagccgtctt taccgagctt
     3241 caaacattct gagtttcctc atacaatgct tgtcggaggc tcagggcagg cgtttcagag
     3301 cgttacttca tcattccacc tgtcagatta tcagttctcc taataaccaa actttttcgt
     3361 tcttttatta gtgccttagc ttttcactaa ggaacgagtc gcacataaat tttgtccgaa
     3421 attcatcttg tcactttcct ttcttttcca ttgctcgatc cgctgctgca cttgcattca
     3481 tcaagcacat cacagtaaca ccaagcatac tgccaccaaa taccaaaaaa atatctttta
     3541 caattcccat gacgattcct cttttcatat aatcaaattt tcaattgcca tactttgttg
     3601 ctccaaaatc tttttatgtc ggtctgataa gcgtgcattc tgtaccatat cctggatata
     3661 ggttgtccga ttcaatcgat ccaactcctt agccattttc cacgtagggg cgacttgtcg
     3721 gcctagccaa ttcaaggttc gttcaatggt atacggtttt ggctcggaag ttaattgaat
     3781 ctctttccga ttccgtccaa taaaacgccc ccattgttca ttcaagggcc agttggttcg
     3841 ccttttacct tctactttat ccgcaaatcg caagtaacga ttgataatgg aaaacgtggt
     3901 gctctcaatg ctacgacctt ttaacaaatc ttgaatcgca tgaaaagcac ggtcattttt
     3961 cagtcgaatc tcaaaacgat ttttcgtctc agtttggtct aatggaatcc ctaacttgat
     4021 atattgttca tagtcttttt catacaaaca gaaataaacc tcactcttga gactgccgat
     4081 atataaggta ttgcccatgc cgtccttttc atcagccttt aaaagttccc cggaacgata
     4141 acttttaaag gtacgaaaca aggagataca ttcttccttc tgacattttt tcgttagctc
     4201 aggtatatcc aatagcccca ctcgatcatt gattgccaag tccaagcgtt tcatcacgcc
     4261 accggccttt agacaatcct cgaaaaagtc gtaccagcta cgcttttgag cgagtaaaaa
     4321 agtttcaaat tgtcggcaac ctcgcccctt taattctaaa agcacacctt tatcttcttc
     4381 atgagacaac atcaccacaa tatcacccat gacatactgt tcttgatagg agtaaaaggc
     4441 ataatcttca tggagcatat actcaaattt caaatggaga atttcttgaa taatttttcg
     4501 tgcatccgtt gtgggaaagc gaatccttac atagtcaaac agtaacgtca aaggcaaatc
     4561 gggattaaag ctttccagtt ggtcaaaaat tttcctcatg gtttttatag atgcggttac
     4621 gccaccattt tctatctgat ttaagtacgg ccgactaagt ccacaagccg tagccaattt
     4681 attttgtgaa acgccgtatt cgttgcgtcg gtcttttaat agccgataat ctaaattccg
     4741 ttgtgccaag ttctcacctc caaattcaag gtgttaactt tttcaaatga gttaacacct
     4801 atacaaatta cttactatct ttgatacaac aagcattctt aatggttgct tacttatttt
     4861 ttgtgttttt gtacccccct gttagatacg ggggtgagac atcatggtcg gctaacgccg
     4921 tccatgaaca ggcacagcac cacggaggat ttctgcttcg cgaatcctcc gcggtcagcg
     4981 cctaacagtc agttgtgttg gtttaggtta ctcatctgta ataccattta cttccaactc
     5041 tgctttcatt tctaacaagt attgacgaat atctggttct tttgcttggt ccgcaatccg
     5101 ctcaaattct gcaattactt gatttatttc aattcgagca tccgtctcct cattcattaa
     5161 atatggatcg ccagcgactt tgacaccatt cttgcgaata tcaaatttta accgtcgcaa
     5221 ttgttcttct aattctgggt ctgactgaat ctttccaatc acccaataaa tttctccaat
     5281 caactcatca taaaccatgt acatccaact cttttaatct tctggcaata tcaaaaatga
     5341 ccggaactgt tacagagttt ccggcttgct tatacaactg actatctgaa ttgacttgag
     5401 aatctcgatc aaaagcccaa tcgggaaagc cctgaagtcg ccaacactct ctaggtgtta
     5461 gcttccgaat ttgatactga tccgtgacga tcgcttgttg gtctcctgtt agtaaggtat
     5521 gaacactctg ttttcccaga cgacctcggc gacttgtgga attaggaaaa gccagattca
     5581 cactgtcttc tggcttcacc tcttgatatt ctgtttcatt ttttcgaatc atgatttttg
     5641 taccaccttg tttcgttgac aaggtggggg cggttccagt aacttcataa actcga
//
LOCUS       DADVXN010000136         5586 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.140, whole genome shotgun sequence.
ACCESSION   DADVXN010000136 DADVXN010000000
VERSION     DADVXN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.140"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(118..576)
                     /locus_tag="KB942_002567"
     CDS             complement(118..576)
                     /locus_tag="KB942_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6852949.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(589..1920)
                     /locus_tag="KB942_002568"
     CDS             complement(589..1920)
                     /locus_tag="KB942_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBB6852950.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1931..3373)
                     /locus_tag="KB942_002569"
     CDS             complement(1931..3373)
                     /locus_tag="KB942_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6852951.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3360..4124)
                     /locus_tag="KB942_002570"
     CDS             complement(3360..4124)
                     /locus_tag="KB942_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBB6852952.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4228..5103)
                     /locus_tag="KB942_002571"
     CDS             complement(4228..5103)
                     /locus_tag="KB942_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6852953.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5358..5442)
                     /locus_tag="KB942_002572"
     tRNA            complement(5358..5442)
                     /locus_tag="KB942_002572"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5406..5408),aa:Leu,seq:caa)
     gene            complement(5460..5531)
                     /locus_tag="KB942_002573"
     tRNA            complement(5460..5531)
                     /locus_tag="KB942_002573"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5497..5499),aa:Gln,seq:ttg)
BASE COUNT         1710 a         1181 c          835 g         1860 t
ORIGIN      
        1 attcgataat taacaaaaat caaaatgatt aaatctagtg attgaaacat atcatttcaa
       61 aaaaattttg taaattcacc cataaatatc accctattta acttttttca tcgtacacta
      121 aaattcaaaa caactatctt taatctttac ttcttggttt tgttttaata aatttccttc
      181 aaaaatgcct ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt
      241 caaatctata atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat
      301 ctgaaacgga cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct
      361 gccagattca ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc
      421 tttaactggc gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat
      481 ttttgaagaa aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac
      541 agcttttaga atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc
      601 tggccaatat tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc
      661 atttggtacg gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa
      721 gtaagcatct actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa
      781 atcactgatt ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg
      841 tatttcaaca actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag
      901 cataaagcga cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc
      961 atgctcccca aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc
     1021 catttctttg gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt
     1081 gtcagtatgc tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat
     1141 attacttgga aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt
     1201 atctcctata ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc
     1261 tggcataatt tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt
     1321 taaaccataa tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt
     1381 ttcttcaatt gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg
     1441 acgaacagct tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg
     1501 ggcatagtct tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata
     1561 agcaaatcct ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt
     1621 ttcgtccttt tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca
     1681 tcctgtaaaa gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt
     1741 tcttttcagc ataaatttaa caatcaagta catatccata tttctttctt catcaatatc
     1801 atataaacgt atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat
     1861 tcctggtgta tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat
     1921 cttttccttt ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta
     1981 agccgatgat aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt
     2041 gcttgattcg ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat
     2101 tacaactata gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg
     2161 ccatatattc ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat
     2221 tgccaaactc tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat
     2281 aataaaaagt aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag
     2341 cattggtagc gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac
     2401 caattcgtat attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga
     2461 tcacgtacaa cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg
     2521 caataaatga agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt
     2581 tataagctgc tttatagata gctagacata tagctggtat accaaacatc attactacaa
     2641 aaaaaactga taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc
     2701 ctctgaaaac ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat
     2761 tcaacacatg gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca
     2821 cacctaatgc tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga
     2881 gcggccagat aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca
     2941 atgggaattt ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc
     3001 gattatacgt ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca
     3061 cttctgggtc cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa
     3121 gtgcaggtat tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata
     3181 agatatccaa gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca
     3241 tatcgtcagc tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta
     3301 gcatcgctct tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat
     3361 taaatatcct catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat
     3421 gctgaatgaa ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt
     3481 aacaattcct gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc
     3541 aatatcatga gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt
     3601 tcatcagttt ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca
     3661 tcttttcgtt caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact
     3721 acctcctgat tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg
     3781 atccccgcat gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg
     3841 tcaatgttaa atggatgctg ataataagat aaggatctga actctccgct ttcgataaga
     3901 gtatcgacag ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct
     3961 agcctaggca tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc
     4021 aatgtagttg atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca
     4081 tggctatagc caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt
     4141 tttctactaa aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt
     4201 gcgcctatag atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata
     4261 gatgatttca ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt
     4321 tagtgctgta caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc
     4381 tactggcgtt tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa
     4441 gcgatgataa gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga
     4501 caaaatcgtg gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag
     4561 ctcagcatta atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg
     4621 atatcgatga ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag
     4681 gaatctcatt tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat
     4741 agcaaaataa atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat
     4801 cctagctgtt atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg
     4861 cgagactatc tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc
     4921 agcatcctca acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat
     4981 cgcaattttt tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat
     5041 cacatacacc gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt
     5101 catacatatt tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc
     5161 tttcttaggt cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa
     5221 cacataataa aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga
     5281 attgatttgg aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt
     5341 tgctatattg ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact
     5401 ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac
     5461 tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg
     5521 gctatacccc aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac
     5581 aatccg
//
LOCUS       DADVXN010000137         5379 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.141, whole genome shotgun sequence.
ACCESSION   DADVXN010000137 DADVXN010000000
VERSION     DADVXN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.141"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(19..294)
                     /locus_tag="KB942_002574"
     CDS             complement(19..294)
                     /locus_tag="KB942_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBB6852954.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(266..1219)
                     /locus_tag="KB942_002575"
     CDS             complement(266..1219)
                     /locus_tag="KB942_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6852955.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1831..3324
                     /locus_tag="KB942_002576"
     CDS             1831..3324
                     /locus_tag="KB942_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBB6852956.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3459..3755
                     /locus_tag="KB942_002577"
     CDS             3459..3755
                     /locus_tag="KB942_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6852957.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3856..4707
                     /locus_tag="KB942_002578"
     CDS             3856..4707
                     /locus_tag="KB942_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852958.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4728..5011
                     /locus_tag="KB942_002579"
                     /pseudo
     CDS             4728..5011
                     /locus_tag="KB942_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1842 a          831 c         1017 g         1689 t
ORIGIN      
        1 tagtatactt actatatttt aatcttttga tcgtctatta tatataatcc gttcctcgtc
       61 gtttaaagta tcaacatagg cgtccattgc aagtttgact atttcataca taggaatatc
      121 ttttgcataa ctaagatccc gaatggtttt tagtacggga ggatctactt gaactggttt
      181 tctttctttc gcagtgtact ttcgttcccc taactcgaca ttaatatcat ctttaattga
      241 aatatcttca gcaccaattt tcttcttatc tttgttcgtt ggtaataatg ccatttagtt
      301 taatctcctt tccaagaggt aggatatgat tggccattga gtcagcatat ttaggtgtat
      361 aatcaaatcc ttcaatatca ccatgttgtt catagtattc aattctttct tctaattctg
      421 taaagatgtc tgcaaaaata gcccaccatc ttcgatctac ataatcattt aattggatac
      481 cattctcacc atacatttct aaccgatccg agcctttaac aatgctttta aaaatgtttg
      541 attcaccata cttatcaatt gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg
      601 agaaaagaac aggcaaaatt ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa
      661 tcggaactaa aactttatta ataaaatctt ctgttccttc cattgcgtac cgttttaatt
      721 cttgcattgc aataatataa tctgcagcag ttaagaatgc acgaaccaca ttatctgttg
      781 atggtgggca gtcgataaag atataatcga aattttttct caattcttct aaaggtttaa
      841 tgaaggccaa tgatctctta cgacgatcag gatatttttc ggacaagtat tcattaagtt
      901 cttcagatcc aggagagcca gccataatat ataaatgtgg acttacttga gtaatacctg
      961 aaacccagtc cccttttttg attgctgcag acatggatcg atcaaagcta gtaatttgat
     1021 acgttgatcc aatgttggaa gtagtatttc tttgttggtc ataatcaatt agcaacacgt
     1081 ctttttcaaa aagttgacta gaaacaatcg ctaataaatc attgtctgta gtcttaccaa
     1141 tgccgccttt tctacctgta agcattacaa cttttcctag tttatctgat gttttaaatg
     1201 cagtccgtaa gttttccata gttccctcca atttcctata ctaaatatat ttagtatact
     1261 tcatatactt aatatattcg atatatctaa tatactccaa attgataaga attcagactt
     1321 tataaggaat atagtggata tactaaatat atttgctata ttcagtatat cagatatatt
     1381 caaaatgtca aaatatattt gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa
     1441 cttaattcta tagaaaccac agataattca ctatttacaa agtagcaaaa gcagtggtac
     1501 tctttaaaca gaaaaattac ataaaaaaaa gagacggaaa taattgattt tagcagaatc
     1561 aattatttct gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag
     1621 tagctaccaa ctacttatat taaaagttga tcggatacct tttccgattg cctttattct
     1681 accaaaaaaa ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata
     1741 ttcttaaaca ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct
     1801 ctttttgttt gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt
     1861 aaaaacggct tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt
     1921 gattccagta ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg
     1981 cgtggtatgg tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat
     2041 tggacaccta atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa
     2101 ggtcattctg agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa
     2161 gagaaccatc aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg
     2221 ctaattttag aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat
     2281 gtcacaaaga aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt
     2341 cgtctacagt tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt
     2401 gctcgattcc cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt
     2461 gctgactgga ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga
     2521 gaacgaaaac taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg
     2581 ttcgatatgt tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat
     2641 aatgtgattt tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc
     2701 gaatacaata tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta
     2761 gtaaagattg tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt
     2821 attgaattgt gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa
     2881 cgacgtggct ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat
     2941 gaatggaaag aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat
     3001 ttgaacctgt caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat
     3061 aagatcttaa atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga
     3121 aatggcggaa tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt
     3181 aaagaaaaga gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc
     3241 ttagtagaag agacatttag acaattatca acccccttga accctcttaa agaagtgaat
     3301 ctttttgaag tagatacagg ttgaaaaata aaaggtgcaa attgccttat acaatatatc
     3361 tatgatactt ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata
     3421 actgcgtatg cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct
     3481 attcacatcc tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct
     3541 gtgagcttta tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag
     3601 ttgcttcttt gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg
     3661 atcaggtata tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta
     3721 acgagttaag aaaaaggaag aagtatatcc agtagtagtt tttatttact taaaaaattg
     3781 gttcattgag caacctatcg ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt
     3841 agggaagaaa tcgaaatgaa atcggacgac tggattgaac acacctggct tcctctgaaa
     3901 agaaatagca aacgaaagaa attttgggta ttgcaattat ggccattagg aaaactttgt
     3961 agttacgggg cacgacttat tcttgattta accgtttact cgattggagt atttggaacg
     4021 gcttattact tgataaatga tcccaatagg attgcagcat cagacatttt tacagtgcta
     4081 atgacgttga tttttgtcat gctattgtct atttttactc aattagtcat gcaatttacc
     4141 aggcaaaaat taacagcagg aaaacttttc aaaagcctat tcgatttaat tgttgtaaat
     4201 gcaattctag cgactttaat aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg
     4261 aaggattttt tattgacgtt tattcacttt gtaaatggac acatgtattt gtttgaagtg
     4321 ggtattattc tcttttttgt cgggtatgta tccacgaaac ttattaaacg ttcaattgaa
     4381 aaagaaattg aaattgacct gattccgaca cttcttggtt atgagtacaa tgttgatttt
     4441 ggaagatatg acgaattgat caatacggat atcgttcaca cgagtatcca tgaatctact
     4501 gttaagggca tcagatctat tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga
     4561 aaagagatct acaattttta tgaagtcgaa gcaagcctac gatatggcat cggaaaattg
     4621 tggttagatg gaagtaatat tcgtcaaaac aaaccataca ttttttggaa cagtagtgat
     4681 ttagaacttc caatgaaaga taaataatta tggagaatcg tttgaaaatg attcctctat
     4741 acaggattgt gcttatagtt ggcatggagt taaaaacctt aaagaaggag cagttattca
     4801 aagtgaaaga acgtcataat cagttactat gtagtaaaac gggtacgatg aacattgaat
     4861 accgaacaga atatttagaa ttaacgaatc aaactgattt tttgttgatt cgaaagcaac
     4921 aagagcaaga aaaaaattaa tggctactta tagccaattg attgatgaat gggaacaatt
     4981 caaggaatct aaaattccag aacgtctcta aaagccttta tttgtaaaga gcataaggaa
     5041 acgtgggaac actttttaaa aaaagtgttc caataggctg tgcctattac gtttctcaaa
     5101 aataaatgtg ggaacaaaat atttaaaaat aggatagtga aaggatagtg aaggatagta
     5161 aaaggagggt agtaggatag caaaaaggat aggctccttt tcgctatcct ttttaaattg
     5221 gaggaattaa tatggaagta agaataagaa atgtggatga gaaaatcgta agagaaattg
     5281 atcgaagatg tgacgaacgg ttctgttgca aagttttaaa tctactatca aataaggtag
     5341 aataatagaa aaagatagca ggaggaatga cgatgaatc
//
LOCUS       DADVXN010000138         5378 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.142, whole genome shotgun sequence.
ACCESSION   DADVXN010000138 DADVXN010000000
VERSION     DADVXN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.142"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..433
                     /locus_tag="KB942_002580"
     CDS             <1..433
                     /locus_tag="KB942_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBB6852959.1"
                     /translation="QEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIK
                     DRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEY
                     IIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            405..1121
                     /locus_tag="KB942_002581"
     CDS             405..1121
                     /locus_tag="KB942_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBB6852960.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            1118..2236
                     /locus_tag="KB942_002582"
     CDS             1118..2236
                     /locus_tag="KB942_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBB6852961.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2226..2846
                     /locus_tag="KB942_002583"
     CDS             2226..2846
                     /locus_tag="KB942_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6852962.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            2973..4142
                     /locus_tag="KB942_002584"
     CDS             2973..4142
                     /locus_tag="KB942_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6852963.1"
                     /translation="MCRVLNILFQYEKIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            4149..4544
                     /locus_tag="KB942_002585"
     CDS             4149..4544
                     /locus_tag="KB942_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6852964.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            4541..>5378
                     /locus_tag="KB942_002586"
     CDS             4541..>5378
                     /locus_tag="KB942_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6852965.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYL"
BASE COUNT         2091 a          632 c         1030 g         1625 t
ORIGIN      
        1 tcaagagtat attttaggtg aagaatatac tatagatgtt tgcttaaact ttgattcgac
       61 accagtaagt attgttccta gaaaaagaat agcaactaga ggtggggaaa tactgaaggg
      121 tcagattatt aaagatagag aactaattga gactacgaaa aagatgttgg aaaaattaaa
      181 tgcaatcgga catattacta ttcaatgtat aaaaaacgaa aagggaatat tttatttaga
      241 aataaatgca agatacggtg gaggagcccc cattagcatt aaatcaggag ctaattcccc
      301 tgaatatatt ataaagatga tagattcaaa aaaaaatttg agatacagag aaaattattt
      361 agataaaatg atagcactga gatatgatca agcgatttat ttagatgaaa aaggaaatgt
      421 atataatgat taaagcggtg atctttgatt tagatgacac tcttgtgtct gaatatgatt
      481 atgtaaaaag tggatacaaa tgtgtagcta gaagattagc agtgcaaaaa attttcccgg
      541 ctacttttga cgaaatatat agagtccttt tagatctttt tgctgaagat agtaaaaatg
      601 tatttaatcg tctatatgac tattttgaaa ttccttatag taaaaaagaa attaaagaac
      661 tggtaaatgt atatcgagaa cacaaaccag acataaattt tttagaaaat gcagatactt
      721 tattaacgga attaagaagt agaggataca agctggggat tattacagat gggtatgcta
      781 taacccaacg aaacaaattg gatgctttga aagtagacta tttggtagat tatatttgta
      841 ttactgacga attaggccgt gaattgtgga aaccacaccc tatctcatat cttttattgt
      901 tagaaaagtt taatttaaag gcacaagagt gtatatatat tggcgataat gagttgaaag
      961 attttataac tgccaaatca ataggaatgc aaacaattaa aatagaaaga gtaaatggta
     1021 tttacagaga aagcagtttg aagttcaaaa gattttacca agctgataaa aaaatacgtt
     1081 cgttatggga gataaaaaat tttttgggag tgatttcatg aaaagtaaaa gaattctatt
     1141 agcttctcca catatgagtg aagaaggata tgaaaaagag tatgtaaaag aagcatttga
     1201 tacaaattgg gttgctcctt taggtgcgaa tgtgaatgga tttgagaaag aattagcaga
     1261 atacgtggga gctagtcatg ctgccgcatt atcttctgga acagcagcta tacatttagc
     1321 tttgaaagcg gctggggtcg gtaaagatga catagtgatt tgtcagtcct tgacgttctc
     1381 agctactgct aatccaattc tctatcaagg agctatacct gtatttgttg atagtgatta
     1441 cgaaacttgg aatatgaaac ctgaacattt gcgaacagca ctagaaaaat atcctaacac
     1501 aaaggctgta ttagtagtac atttgtacgg tttatcagct aatttagacg aaattactaa
     1561 aatatgcaaa gaatacgatg ttactctaat tgaagatgct gcagaatcat taggaacaac
     1621 atataaagga aaatatactg gtacatttgg cgactatgga atttattctt ttaatggaaa
     1681 taagataatc acaacttcag gtggcggaat gcttgtatcc aatgacaaag aaaaggtgga
     1741 aaaagtgaga ttttgggcta cgcaatctcg tgagaaggca cgccattatc aacacaatga
     1801 aattggctat aattatcgaa tgagcaatat cgttgctggt atcggtcgag gacaattgaa
     1861 agtattaaat gaaagaattg agaaaaaacg atatattcat agtttttata aaaaagaatt
     1921 gtctaaattg tcaaatatac aattcatgcc agaaaataat tggaatgaac cgaattattg
     1981 gttaagtgtc ataacaataa agggaataaa tgcgaatcat cttattgatg agttagacaa
     2041 aaataatata gaagcccgcc cagtatggaa acctatgcat ttacaacctg tttttaaaaa
     2101 ttatgattat attggcgatg atgtagcaga aactttattt gacttaggag tatgtttacc
     2161 atcagataca aagatgacag atgaagattt aaaatatgta gtatctacga ttaaaagagt
     2221 gatcaatgat gcgtaaaaaa actttctatc agctttttgg gaaaagaatt ttagatatcc
     2281 tattgtcagg aattgctctc atcgttttat cgccgataat cttaattgta ggttttttag
     2341 taagaattaa acttggttct ccaattatct tcaaacaaga acgaccagga aaaagtgaaa
     2401 aaattttttc catgtataaa tttcgtacaa tgactgacga aagagaccac aatggagaat
     2461 atttacctga tgaaattcga ctaactaaat ttgggaaaat gcttagagca acaagtctgg
     2521 atgagctacc cgaactttgg aatattttga aaggtgatat gtcgatcgta ggacctcgtc
     2581 cattattagt ggagtatttg ccactatata gtgagaagca acggaaaaga cacaacgtaa
     2641 gaccaggatt aactggactg gctcaggtca atggaagaaa tgctattagt tgggaagaaa
     2701 agtttgattt agatgtttat tatgttgata aaattagttt ctttaatgat cttattatta
     2761 taattcagac atgtaaaaaa gtaatcaaga aagagaatat taatacaatc aataacaata
     2821 tcccagaaaa atttaaaggg agttaaatac agcagcttaa tgcaagtttt gtgacttgca
     2881 ttatatttag gtataaatca aagcgagtat attagaatta tataacatat gttaaaaaaa
     2941 cgtatagcta ataaaagtat gattttaaaa atttgtgtag agttttaaat atactgtttc
     3001 aatacgagaa aattataaaa aagtataagg aaaagagagt tatggcaaag tattataata
     3061 aaaaaattct tataatggca aacaacgatg atggtcttta ccgttttcga ggaatgctca
     3121 taaaaaactt agtagatcaa ggtaatgaag ttattgtatc tcttccaaag ggcaagaata
     3181 ttcctaaatt aattgaactt ggatgtcaaa ttattgaaac tccaatagat agaagaggaa
     3241 ttaatccaaa aactgacttt aaactatttt ttaactattg taatattttg aaaaaaataa
     3301 agccagatat ggtcattgct tatactatca aaccaaatat atacggtgga ttggcatgtc
     3361 ggttaagaaa ggtttcttat gcattaaata taaccggatt aggaacggca tttcaaaaaa
     3421 agggggtttt gaaaagtctt attgtatttt tgtatagatt agcagctaaa aaatctaagg
     3481 tcatattttt cgaaaatttt gaaaatatga aaacatttca gactttacaa attgctaaac
     3541 aaagtaaata caagctatta aacggtgcag gagtagatgt agaacacttt agttatttag
     3601 aatatcctaa agatgagcaa gttcattttt tatttattgg aagaattatg aaagagaaag
     3661 gtgtagatga actttttgaa gcaatgaaaa acttatatag tgaagagaaa aattgtgtac
     3721 ttgatgtcct tgggtatttt gaagaagatt acggcataaa aattgaaaaa tacgaaaaag
     3781 agggatggtt aaaatatcat ggatatcaat ctgatattcg tccctttgta gaaagagcac
     3841 attgctttgt tttgcctagt tggcacgaag gaatggcaaa tactaacctt gaatcagcag
     3901 catcagggag accaattatt acaagtgata tcccagggtg taaagaagca gttattgaat
     3961 cgaagtctgg ttatttatgt gaaagaaaaa atagttgtag tctctataat tcgatgaata
     4021 aatttctaaa attatcatat gagcagcgca ttaacatggg aaaaatgggg agaaattata
     4081 tgattaaaaa atttaataag aagaaagttg tcgatgaaac aattaaatca ttatttagtt
     4141 aaaaagatat gttttgcaaa gtatgtgata gaaaagaggg attctgtggc ttcagaatag
     4201 atatcaaaga attggcaaaa gtttctgata tttttttatt tactacatta caagaaggaa
     4261 tgccaagatc tatgatggaa gctatggcaa gtggattgcc atgtatagca agtaaaatcc
     4321 gaggtaatgt agacctttta gtggaaggaa aaggtggata tcttgttcca gctaaagatg
     4381 cagaattagt agcagagtgt ttgagagaac taatcaataa taaatctctt agaaagtata
     4441 tgggaaatga gaacctttcc agaattaaag aattcgaggt tagtatcgta aaaaaaagaa
     4501 tcaaagaaat atatgaagaa gttataggtg agagtgtaaa atgaaaaatc caatgttttc
     4561 aataatagta cctatctatt gcgttgaaaa atatttgaaa gattgcttag attcaataag
     4621 aaatcagaca tttaaaaatt ttgaagcaat actagttgat gacggtagtc ctgacgaatg
     4681 cccagaaata tgtgactatt attcccaaat agatggacgt ttaaaagtaa ttcataagaa
     4741 aaacggtgga ataatagatg cacggaaaac aggagtcact agtgctatgg gagagtatat
     4801 agtttttcta gatggtgatg attggcttgc aaatacttat ttagaaaaat atgcggaagt
     4861 aattactaag tacaatccag atataatatg tagtgggtat tttgcatcac gttccaacaa
     4921 aattagaaaa agagaattgt cttgctcgtt tggatattat gacaaagata aaattataaa
     4981 tttgatttat cctaaattaa ttgaggatga acatggagag tattttagac cacaattatg
     5041 ggcaaaagct tttagaacta ctctgtacaa ggaacaacaa caagtatctg ggcaagttag
     5101 tgttggtgaa gatcatgcat gtacaaaacc atgtatttac catgcaaatt cgatatatat
     5161 gatggaaaaa tgtttatatt attacagaat aaacgaaaat tctataacaa aaaaaccaca
     5221 accatattat tgggaaggac caaaggctat tggagagcat tttgaaaagc agattgatat
     5281 gaataagtat gattttcaag atcaagtata tagaaatgtt acacacaatt tatttaatgt
     5341 tgctgtttca caatttaata aacaggacaa atatttaa
//
LOCUS       DADVXN010000139         5336 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.143, whole genome shotgun sequence.
ACCESSION   DADVXN010000139 DADVXN010000000
VERSION     DADVXN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.143"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..546
                     /locus_tag="KB942_002587"
     CDS             <1..546
                     /locus_tag="KB942_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6852966.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="KB942_002588"
     CDS             complement(716..1678)
                     /locus_tag="KB942_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6852967.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="KB942_002589"
     CDS             complement(1680..3119)
                     /locus_tag="KB942_002589"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBB6852968.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="KB942_002590"
     CDS             3304..5250
                     /locus_tag="KB942_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBB6852969.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1718 a          913 c          889 g         1816 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttattt
//
LOCUS       DADVXN010000140         5321 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.144, whole genome shotgun sequence.
ACCESSION   DADVXN010000140 DADVXN010000000
VERSION     DADVXN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.144"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..497)
                     /locus_tag="KB942_002591"
     CDS             complement(<1..497)
                     /locus_tag="KB942_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBB6852970.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(581..796)
                     /locus_tag="KB942_002592"
     CDS             complement(581..796)
                     /locus_tag="KB942_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852971.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIESIKYFV"
     gene            complement(806..1621)
                     /locus_tag="KB942_002593"
     CDS             complement(806..1621)
                     /locus_tag="KB942_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6852972.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1883..2326)
                     /locus_tag="KB942_002594"
     CDS             complement(1883..2326)
                     /locus_tag="KB942_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBB6852973.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2460..2798)
                     /locus_tag="KB942_002595"
     CDS             complement(2460..2798)
                     /locus_tag="KB942_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6852974.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2786..3163)
                     /locus_tag="KB942_002596"
     CDS             complement(2786..3163)
                     /locus_tag="KB942_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6852975.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3387..4580
                     /locus_tag="KB942_002597"
     CDS             3387..4580
                     /locus_tag="KB942_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852976.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4746..5168)
                     /locus_tag="KB942_002598"
     CDS             complement(4746..5168)
                     /locus_tag="KB942_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6852977.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1547 a          997 c          931 g         1846 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcgatgca acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc
      661 catctatcga caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa
      721 ggctacttgc caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa
      781 aacgctgctc tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta
      841 caaaatattt ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg
      901 cagccatgat taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact
      961 ggattgtttt attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg
     1021 tattaacgaa tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga
     1081 tcttgcgata gattccccac tctgataagc catcgatccg cgcactctca cataaagaat
     1141 ctgggatact actgtagtat tgcttcaaca agaacacacc aaaagcattg aacgcttgta
     1201 atagcaccaa cgaccacaac gtatatgtta aacccacttt tctcatcata atgaactgcg
     1261 ggatcatata agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat
     1321 cgcgcccttt aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact
     1381 gaatcaacgt gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa
     1441 tacgagacca aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg
     1501 gaatcgtgaa cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa
     1561 tgatcgtcaa taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca
     1621 tgttttttga acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat
     1681 cttcatttct ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc
     1741 gttcttctta tgtttgtaag cgcttattta acaagttatg taaatctctt tatttttttt
     1801 gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa
     1861 tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt
     1921 cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa
     1981 tcattgggtc aagcggaacg aatgtcactc gctttgaaag atgacttaca gcaccttcta
     2041 tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt
     2101 acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct
     2161 gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca
     2221 cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg
     2281 gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta
     2341 acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt
     2401 gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaattat
     2461 cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga
     2521 attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa
     2581 taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt
     2641 tcctcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct
     2701 gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac
     2761 tccatagcga tgctccttga tttttttact tgatacattc cattttattt ctagataaca
     2821 cacgatcata tttcttgatt tttccttgta aacgttccaa acactcttgc atttcagcaa
     2881 tttctttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg
     2941 cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat
     3001 ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc
     3061 cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat
     3121 cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct
     3181 taatttcttg ccactttact tatgtatttt atttattcta caactttaag tgtactccaa
     3241 gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta
     3301 tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg
     3361 gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta ttattatcga
     3421 catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt
     3481 caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta
     3541 atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg
     3601 tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg
     3661 cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag
     3721 aatacggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag
     3781 cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg
     3841 ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt
     3901 tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat
     3961 ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa
     4021 catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt
     4081 ggctgccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga
     4141 tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata
     4201 aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc
     4261 tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact
     4321 ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa
     4381 tccgcgcgac ggcgaataat ttcattatga accttggacg agctgttggt ggtttttctt
     4441 ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga
     4501 gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa
     4561 gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggcaaaa
     4621 gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg
     4681 gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc
     4741 ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga
     4801 aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga
     4861 tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa
     4921 ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc
     4981 ttcaaaatga ccttatcagg atatttttgg aacaattctt ctaaatctgt tagataattc
     5041 aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt
     5101 tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa
     5161 gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga aaaaaaacgg
     5221 atacaaatat catattgtca caccgtcaat agtgcataaa cctgttatta tttctctttt
     5281 tttcctaagt attctattga tatgaatcat ttaatcgttt a
//
LOCUS       DADVXN010000141         5304 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.145, whole genome shotgun sequence.
ACCESSION   DADVXN010000141 DADVXN010000000
VERSION     DADVXN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.145"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(66..1214)
                     /gene="nagA"
                     /locus_tag="KB942_002599"
     CDS             complement(66..1214)
                     /gene="nagA"
                     /locus_tag="KB942_002599"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBB6852978.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1290..2126)
                     /locus_tag="KB942_002600"
     CDS             complement(1290..2126)
                     /locus_tag="KB942_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6852979.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2310..3527)
                     /locus_tag="KB942_002601"
     CDS             complement(2310..3527)
                     /locus_tag="KB942_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6852980.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3676..3855)
                     /locus_tag="KB942_002602"
     CDS             complement(3676..3855)
                     /locus_tag="KB942_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852981.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3910..3993
                     /locus_tag="KB942_002603"
     tRNA            3910..3993
                     /locus_tag="KB942_002603"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3944..3946,aa:Leu,seq:gag)
     gene            complement(4233..4811)
                     /locus_tag="KB942_002604"
     CDS             complement(4233..4811)
                     /locus_tag="KB942_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852982.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4817..5200)
                     /locus_tag="KB942_002605"
                     /pseudo
     CDS             complement(4817..5200)
                     /locus_tag="KB942_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1652 a         1081 c          838 g         1733 t
ORIGIN      
        1 agaacacaaa aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa
       61 ttatattatg cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca
      121 gggttcaata cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg
      181 attttacagc tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct
      241 aatccccagt ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag
      301 ttgccagatt ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg
      361 taatttccat caggcatccc accggccatc atacagtctg taattaatgc gacatgatca
      421 tgtcctgctt tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa
      481 atcaattcag aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga
      541 tgattcaatc cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca
      601 gctttttgcg cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct
      661 tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt
      721 ttgattaaac cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg
      781 aaataacttg ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag
      841 attcctttga ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca
      901 tcttttaaac gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa
      961 gataacagac cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga
     1021 ttcatatagc cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg
     1081 tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg
     1141 gtaatttcaa gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag
     1201 ataaaagttc tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta
     1261 gagaagaagt caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct
     1321 tgtgctaagc cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag
     1381 cttttaagca caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag
     1441 attccttgat tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt
     1501 ccataattat aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc
     1561 agcagttgga tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca
     1621 tatatttttc cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct
     1681 ttttggctag agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga
     1741 cgcaacgtga attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga
     1801 tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt
     1861 ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga
     1921 gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc
     1981 aatacattcg gcagttcctc attattttcg atccgactta atacactttg tgaacaaata
     2041 tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt
     2101 ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc
     2161 tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata
     2221 tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc
     2281 cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct
     2341 ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa
     2401 tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt
     2461 aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt
     2521 gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt
     2581 gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg
     2641 acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac
     2701 aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct
     2761 ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg
     2821 ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca
     2881 tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct
     2941 acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga
     3001 ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca
     3061 ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca
     3121 atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc
     3181 agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca
     3241 ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca
     3301 gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca
     3361 cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt
     3421 aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca
     3481 tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc
     3541 cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata
     3601 ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa
     3661 acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat
     3721 tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc
     3781 aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt
     3841 aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt
     3901 tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt
     3961 tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt
     4021 cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt
     4081 acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa
     4141 gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc
     4201 acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag
     4261 gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg
     4321 cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata
     4381 tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa
     4441 caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt
     4501 aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa
     4561 gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta
     4621 ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt
     4681 gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca
     4741 taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat
     4801 aagatattca tgtctaaaaa aacggctttc caaatgatga aatacctaaa tctaacttat
     4861 taagaaagtt gaaattttta tattcaggaa accatttatt aatttcatgt gcagcacgta
     4921 atgcattccc tggagttgtc aaagagaaaa ttaattctgc taagcttatt attgaaaata
     4981 ctgataactt ataattatat cgtccattga caatcaaata taaactaact atacttgtta
     5041 gaacaaaaaa acaggcgttg acttgttctt gattagttaa aaatatcaat aaaggaatac
     5101 ttacccaata tatttttcta tttatttctt ctcctcttaa gagttgaaaa aacggataaa
     5161 aagttagtaa actaaatgct actggccact gatagttcaa ggtggttgct ctccaaccgg
     5221 tttcccccat tactgtcaat ggtattaaac agtatataga gcaaaccaat aataaatttt
     5281 taacatttat tttctcaaaa acat
//
LOCUS       DADVXN010000142         5166 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.146, whole genome shotgun sequence.
ACCESSION   DADVXN010000142 DADVXN010000000
VERSION     DADVXN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.146"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(599..889)
                     /locus_tag="KB942_002606"
     CDS             complement(599..889)
                     /locus_tag="KB942_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852983.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1291..1527
                     /locus_tag="KB942_002607"
     CDS             1291..1527
                     /locus_tag="KB942_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852984.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1534..3486
                     /locus_tag="KB942_002608"
     CDS             1534..3486
                     /locus_tag="KB942_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBB6852985.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="KB942_002609"
     CDS             complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="KB942_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6852986.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4382..5074)
                     /locus_tag="KB942_002610"
     CDS             complement(4382..5074)
                     /locus_tag="KB942_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6852987.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1668 a          901 c          860 g         1737 t
ORIGIN      
        1 aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta
       61 gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac
      121 aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa
      181 atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt
      241 tacttttacg actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt
      301 ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc
      361 tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc
      421 aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt
      481 agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt
      541 ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct
      601 agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta
      661 ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat
      721 tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac
      781 tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac
      841 taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata accttcacct
      901 caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt
      961 gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac
     1021 atgtactaaa aaaaagaggc agcagtatag atttaagtag ttcagttttg gagtacaaaa
     1081 agtgaaaatg taaacctaaa tgagtatcaa attgcaactg aatttgatac tcgtttttta
     1141 ttgttaaatc aacgtttgtt gttaatttac accacaaaat gtaagttaaa atgtatataa
     1201 aaatgcgaac gtgaaataat tattgtagga atgcaaattg gtgtgttaaa atggttacaa
     1261 tttactaagc aaaaaggggt gcattttcct atgcaatttc ttgagccaaa aaataagaac
     1321 gctaagtctg tggattggga aatatcagag caagtaaggg tgatagttaa gcaatacgct
     1381 gaatatgcag agcgtactga aagcgaagca gtggatgaat tcttattgaa cattttagac
     1441 gataagaagt ttattgaatg gattgccaac aagcgaagta ataagcggat tgttgaaaaa
     1501 atgggtatca aagatagagt gggttgatag acaatgacca agcttaaaag attagccacg
     1561 aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg
     1621 gctaaacaat atgatttatt aactccaaaa cgatttgcaa caaggtataa caaaatgttg
     1681 ttcttgccaa cgtcttttaa atggaacggt tctgaatatc ccattcaata taattactgc
     1741 atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg
     1801 aaaccaagtc gatataaaat gactggtagt tccaaagata aaggtcataa aggtatgtac
     1861 tgcaatgaca atcctattgg cagaggtgta tcgcaaaact gtacagtaac ccctttatct
     1921 aattggtctg ttgttgaaga aattaaacgc cttatagaaa tcaatagcat ccaagatgtt
     1981 gagccagatt atcaatttca taaagaaggc tgctcggaag aagaatcaac cccattcaat
     2041 gagccgaagc aattttacaa aagggggaaa agtaggggca aatctcaacg ctatcaatgt
     2101 aaggcgtgta agaagttcac taatgttcta ccaaaaagag aagaaaccac tacttatcac
     2161 caacaaaaga acaccattct tccaatgttc gctaagatgg ttgttggaag ggtttcagtc
     2221 agtcgtactt gtgatatttt aggaattgga gttggaacct actatcataa gctggagtgg
     2281 ctgtataggc gttgtttgga gtttttagaa cgatatgaga cacaacccct tcaaacaaag
     2341 aaatttaacg agatgtggct taatacggac aagatgcact actacctaaa taacgtccgt
     2401 aagaaaggac aaggttcaaa gaaatatacg gggtttgaag accttaatat gcaaacgtat
     2461 atagttgtat ctgctgaggt cttatcaaga tatgtgttcc gttctgatgt tgcctatgat
     2521 tggaatattt caatggatga attaaatgaa gatacaagaa aattcaaaga agaccatctc
     2581 aatactttta gtcgtaaaaa tgataggtta gattggtctt attatccaca agaaccatcc
     2641 gctaacgata gcgaaaatag gaatgcttat cttcacgaat tagggaaaat tactaatagg
     2701 agtagatttg tcgatggttt aaatgttgat gctccttata ctacaactgc tcactattgg
     2761 ttaattaaac aaatggttaa tgccgacgaa tggcgaatga ttagtgatga tgacttctct
     2821 attagaaacg ccttttatag agtattcacg aaagaattaa ggttgagcga tgcacatcat
     2881 tttatttgcc aagttaacaa gacgaaaagc agaaaacaat gcttaaaaga attcggacaa
     2941 gcaaaagcag aactgctcga ttggggagat atcagaggat tcaaaacaaa gtttctacgg
     3001 actttggcat ctcattatct aactgaatta ctaacaagtc atcaattcca cgaagaggct
     3061 attagtaaag atggagaaag atatcgcaaa tatgccgata accctatcaa acatccatta
     3121 gccactaagg ataaaggatt ttattcagtt gattgtagaa cggatttatc tgctctggaa
     3181 ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc attcgaccaa tagctttatt
     3241 caacagataa gacgttatat atcatctctt gaaagaccat taacaacagc tcgtggtgat
     3301 aagaagagct atatttacgc taacttcaat ccgaagtatg ctcaatttgc gattacaatt
     3361 ctaagaactt actacaactt ttgtcgccct tttaaatcag cggataaaaa ggttttgaca
     3421 cctgctcaaa gattagggat tacagataaa caatttgatt ggaaagatat aatttatttc
     3481 aaatgacaaa aagagcttgg agtaatccca agctcttgtg ctttatcttt actgttcttc
     3541 ttcaattaac gcacccgtta actcaataag ttttttcttt catatacatg aaaatagtag
     3601 ttatacggat ttttatcatt ctttatccct ttttgggcaa atacctcatt ccattcctca
     3661 taattcactt ctggaaaaaa agtatctcct tcgaattcat gatgtatttt tgtgatgtac
     3721 attttctcaa cataagggaa aaacaaatta taaatctgtt ctcctccgaa aataaaaatt
     3781 tcttcttcgt ttttacataa ctcaaaaaca tcttctattg aatgaacaat ttcacaacca
     3841 ttaaaggtaa accccttatc tctcgtcaga ataatatttc ttctgtcagg taaggctctt
     3901 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
     3961 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
     4021 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
     4081 ttaccatcta atttcttatt tctatcaaaa aaccttttct cattatcctg cccgttagtt
     4141 gaataagcaa ttttttctat aagttatatt atcataatac cacaaaatca caaattttca
     4201 ttcaattatc attttcaatt gcattttata aatcattttg tatgtaattt ggtatgtaag
     4261 ttaaaatgaa aaaagtagtc cattttgaag tacattttgt cattttgtac tccaaaactg
     4321 aactacttag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     4381 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     4441 catcttttgc tttttctaac ttttgaatat attgtttcca gcttgagttt tcagcagttg
     4501 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     4561 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     4621 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     4681 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     4741 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     4801 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     4861 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     4921 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     4981 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     5041 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     5101 ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     5161 aatttt
//
LOCUS       DADVXN010000143         5093 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.147, whole genome shotgun sequence.
ACCESSION   DADVXN010000143 DADVXN010000000
VERSION     DADVXN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.147"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            127..270
                     /locus_tag="KB942_002611"
     CDS             127..270
                     /locus_tag="KB942_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBB6852988.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            848..2431
                     /locus_tag="KB942_002612"
     CDS             848..2431
                     /locus_tag="KB942_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6852989.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2868..4052)
                     /locus_tag="KB942_002613"
     CDS             complement(2868..4052)
                     /locus_tag="KB942_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBB6852990.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4077..5084)
                     /locus_tag="KB942_002614"
     CDS             complement(4077..5084)
                     /locus_tag="KB942_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6852991.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
BASE COUNT         1561 a         1079 c          967 g         1486 t
ORIGIN      
        1 tttaaaataa agggaaaaca gtcaatgagt tcaagtaaaa taaaaaaaag tgaacaccag
       61 ggcgattagt cgccctggtg tggtttaaaa cgacctaagc atgccaaagc ttattggttc
      121 gtcaatgtgg aagtttccga tgaatctgat acgaaaaaat atgaagtatc gatggcggat
      181 accgatttta tcgccaaact aaaattttat ttcgggttcg ggtaccgcga gaaggcaagt
      241 gctacattcg gtaaatcatt caagaattga tgaaaggcta ccgcttgcca ttgcaaccac
      301 aacgatactc ctgaacgcga tgttggtgag atttccaaag agaagagctg tttaatgcaa
      361 cggtattaag caaatggcaa aaggaagtca tgtagacaaa gttttttatc aaacaccata
      421 tgatttcgcc tgaacgctgg tttagctcag aatacaatga tgtggtccac gagacattac
      481 cctcagtcag ataaaaaaag ctctctgaca gaattacaca tcgataaata ttaagtaaat
      541 gtcagcgcta gttgaacttt ctgacacttt caggtacact ggaaaagaca actacacgaa
      601 ggagggaaaa tactatgatg cgcatgacta aaataacaag taatgaaaat taccaacgat
      661 accatatcgc atttagccat gcaattggtt ttacagttgc tgttgacggg acaagtttgt
      721 ccaaaacagt tgaattcacg taaaatcaat ggccatctca aggtattttt ctcatgataa
      781 tctgagttta aataacacag gccatggata accatggcct ttttgagtgg tctcaaggag
      841 agagatcatg atcatacaag caatttctat taaaaaaaca atcggcggga atccattatt
      901 tgaggattta tcctgtcaaa ttgaagctgg tgaaaaaatt gtcttagtcg gtatcaacgg
      961 aactggcaaa tcaacctttt tacaaatctt gaccggcaga gaaggtgtcg acagcggtgt
     1021 tatcagtcgc aaaaaaggct taaagatcgg gacggtcgaa caagaactta cagtttccga
     1081 agcgaccgtc aaccactact tgctccattc tgccgctgag atacaggacc tcaaacagca
     1141 aatgagtcgc tacgaagcat tgatgacaga accagataca aacttagaga agtgtctggc
     1201 ccgttacggt gagctgcagc atcgctttga agaactaggc ggttacgtat tagaagaccg
     1261 gattgcagcg atcttgcaag gcttagggat tccacaccgt caggaagcac ggttaacaga
     1321 acttagcgga ggcgaacgag ttaaagtcgc tttagccaaa attttagcag ccgatgccga
     1381 tttactttta ctcgatgaac cgaccaatca tttagatctt aacagtattc gctggttgga
     1441 aaactattta cagaatacaa aaaaatcggt tctggtcgtt tctcatgatc ggcaatttct
     1501 cgatcaagtc accacaaaaa catgggaact ggaagaaggt gcattgattg agtatcccgg
     1561 aaactacagc cgttttcgag tcttgaaaga agctcgcttg gctgagctga caaaaaatta
     1621 cgaactgcag caaaaagaag tccagcggtt gaaagtcatg attcgtcgtt ttcgtcagtg
     1681 ggcacatgaa ggagacaatg agtccttctt caaaaaagcc aaagaactgg aacgccgcct
     1741 ggcgaaactg acactcgtca aaccgccacc tcccccgaaa aatcggctgc aaagcttgtc
     1801 taatggtgga aaatctggca aagaagtttt catcattcaa aacttgcacc agcagtacgc
     1861 tgatcaagta ctattcaaag acagcagttt tgcagtctat cgtggagatc atttagccat
     1921 catcggcgac aatggtgcag gaaaatcaac cttactcaaa ttattactcg gtgaggaaaa
     1981 gccttacggt ggcaccatca aattaggtag tagcttgcaa atcggttatt tgccgcagca
     2041 gctgcagttt gccgatcccg atagccgttt gctggcttac gccatcacat tgactggaaa
     2101 cgaagaagtc gctcgacggc aattagccaa aagtggcttt tatcaaagcg atgtgggtaa
     2161 acggatcaaa gatttatccg gtggtgaaaa aatccgtttg gcgttgctga aactcttttt
     2221 agaaaaaatc aacgtcctga ttttggatga accaaccaat cacctcgaca gctatgctcg
     2281 agaagaaatt gaagaaatgc tgcaagacta tcaaggaacg ctcctagcag tcagccatga
     2341 ccgctacttt ttgcagcaac acttccaaga agcactcgtt attgatcagg agcagatcag
     2401 ccgccagccg ctcggccaac tgattgacta ggacgttttt tcgcaacaaa aaacttgatt
     2461 ttcatacctg tgctttcacg attcaggcaa agtacaggtg gaaaatcaag tttttattat
     2521 tcgatcaatg aatgtttcgg atcggttttc tagctaaaca taaagcgaag aataaagtcg
     2581 acagcccacg aggctgatct aaaaatgttt tttcttttta catatgtaat gtcccttata
     2641 aagatgaatg aaaataaaat attgggattt tcctgaaaat caataaataa aatgccagtc
     2701 ttttcccttt tgaccacttt tccaagcaaa attccctaag aagatcctgt aaaaaagaag
     2761 ggaaattgaa gttaaattac cattttgcgg tcttctccgc aaaaaaaaca catgcaagaa
     2821 ttccctcttg catgcgtttc atcttttatc ctaacgaatg ctttggctta gccgcgcaga
     2881 cgctccacat cacgggcaat cattagttcc tcatctgttg ggatcactaa tactcttact
     2941 tttgattctt ttgtagagat atctacttct tcaccacgca attcattcaa cgctggatcg
     3001 atttcacaac caaaccaaga catacctttg atgatttcac tacgtacatg tccgtcattc
     3061 tcgccgatac cagcagtaaa gacaattgca tcaacaccat tcatcgttgt cacataaccg
     3121 ccgatgtatt ttcggatgcg gtccacgaaa atgtcataag ctaaacgaat atgttctttt
     3181 tcatagttct tttcaagatc acgcatatca ctagacaatc ctgttaggcc tagcagacca
     3241 gatttcttgt tcaaaacatt gatcatttca tcaatatcaa tctctaattt ttccattaaa
     3301 tatgggagaa cagctggatc gatatcacct gaacgtgtac ccattgtcac cccagctaat
     3361 ggtgtgaatc ccatagaagt atccatggat ttacctccat cgactgctgt gatcgataca
     3421 ccatttccta agtggcaagt gatgattttt agttcttcga tcggtttgtt caataatttt
     3481 gccgcacgtt ccgacacata acgatgactt gtgccatgga agccatattt tcttactttg
     3541 aaatcttcat aataagacac cggtaagcta tataaataat tttgctttgg cattgttgag
     3601 tggaaagatg tatcgaatac agccacactc aaaatgtcag gtaaaatctt tttaaacgct
     3661 ttgatcccca ttaagtttgc aggattatgc aaaggagcaa attctgctaa ttcagcaatg
     3721 cggtccatta ctttctcatc gatcactact gaatccccat aagtttctcc tccgtgaaca
     3781 acacggtgcc ctactccggt gatttcatca taagagctta aaatttgcag ttcaatcaac
     3841 tgatctaaca gcatttttac tgctacttcg tggttttcga tatccatgat ttgttcgaat
     3901 ttttgattat cgccgtactt gatggtaaaa atagaatctt gtaaaccgat acgttcgacg
     3961 atccctttcg caactacggt ttcttctggc atttgatata attgccattt caaacttgaa
     4021 ctcccagcat tgattgcaat tgttttagac atatgactct ccttctcgtt gaatgattac
     4081 agagcagatg atttccagtt tttaaactcg ttgaaaaatt ccgtcacctt ctctggttct
     4141 tttaatgaag caagtttcac aagcaagact tcttttactt gttgtgcttg tggtcctttt
     4201 ttctgtaaga tcaaaatact tttttgcgat tgtttattac ggaacaattc atctggcaat
     4261 tgaatcattc cttgaagata accttcttct ttaaaccatt ttttgatttc ttcactttga
     4321 tccgtttcta aaaatccact cggcatcaaa aacaaaccaa aaccatccgg cttcacatat
     4381 ttcattgatt gctctagcaa taaatgatgt gcataactat ggccttcttc ggtacttgtt
     4441 aaaaactctt ttgctttttc gtcattcgga taataaccga ttggcaaatc gctgattgca
     4501 aaatcgacag gatctattaa taattcctgc aacccgtctt gatgaaagta ttgaacattt
     4561 gcttgtgtca aatcacttgt agaagctgcg acagccaaca atgtatcatc aatatccaca
     4621 ccaattcctt ctgtttggta ccccgcattc gataggttta aaagaactgt taataacaag
     4681 ttgcccattc ctgccgcaat atccagaatc ttaaccggtg cttttttatt tgtgtatagt
     4741 tgttcaataa gaaaaacaaa taaaaaaccg atactatcag gagtgagttg atggttcgcc
     4801 tgcaaatgtt ctgtctggct gcccttcaat aaaagtaatt gagacagtct gcgccattct
     4861 tctggctcga aagaaagttt cttcaactct tcataaagag ctgtgatccg ttgcgttgtc
     4921 tcttttgtcg gcactccatc caccacacgt acttgataat catctattaa attttctgca
     4981 ttttcgacat aggcttctaa aaaggaagtt cccaatgcat tctgtaaaag ctgaatagct
     5041 tccaggtttt gattaaaccc ttgttccatt ttttctggta acatggtgca cct
//
LOCUS       DADVXN010000144         5039 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.148, whole genome shotgun sequence.
ACCESSION   DADVXN010000144 DADVXN010000000
VERSION     DADVXN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.148"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="KB942_002615"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="KB942_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBB6852992.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="KB942_002616"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="KB942_002616"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBB6852993.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="KB942_002617"
     CDS             complement(2169..2906)
                     /locus_tag="KB942_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6852994.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="KB942_002618"
     CDS             complement(2997..3524)
                     /locus_tag="KB942_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6852995.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="KB942_002619"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="KB942_002619"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBB6852996.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="KB942_002620"
     CDS             complement(4401..4886)
                     /locus_tag="KB942_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBB6852997.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1125 c          863 g         1714 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgtttt
//
LOCUS       DADVXN010000145         5023 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.149, whole genome shotgun sequence.
ACCESSION   DADVXN010000145 DADVXN010000000
VERSION     DADVXN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.149"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..582
                     /locus_tag="KB942_002621"
     CDS             <1..582
                     /locus_tag="KB942_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6852998.1"
                     /translation="PDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASV
                     GMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQ
                     REDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPS
                     ICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            592..1386
                     /locus_tag="KB942_002622"
     CDS             592..1386
                     /locus_tag="KB942_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6852999.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1399..3165
                     /locus_tag="KB942_002623"
     CDS             1399..3165
                     /locus_tag="KB942_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBB6853000.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3188..4996
                     /gene="uidA"
                     /locus_tag="KB942_002624"
     CDS             3188..4996
                     /gene="uidA"
                     /locus_tag="KB942_002624"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBB6853001.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1816 a          601 c         1006 g         1600 t
ORIGIN      
        1 cctgattact ataatgatgc acagggaata gttttaatga attttatcaa tcctattttg
       61 gttttaatag ttgtttcgcc tttaactttg tttttaagta aaaaatatgg aaaaaaagaa
      121 ctggcatcag taggaatgtt cttttcagca atagtatatt tgatcttatt tttgataaaa
      181 gctagtaata tgtatgtatt cttaattctt agttcggttg gctatatggg tttgggtgtg
      241 tttaatactg ttatttgggc aaatattact gatgttattg atgatcaaga agtaaagtct
      301 gatcaaagag aagatgggac cgtatatgcg gtatattctt ttgctagaaa aataggtcaa
      361 gctctagctg gaggcgtcgg aggttggact ctttctatta taggctatga ctctttagca
      421 aatgtacaaa cgggagatgt tttagaaaag ttatataacg cttcaacatt aattccatca
      481 atttgttttc tgattgtagg attaatttta tttttccttt atccattatc taaagataaa
      541 gtcttagaaa attgcgagct tttaaaacaa aaacataaat aggagagaaa tatgaataat
      601 tggatatttt gtattgattc agatggctgt gttatggata cgatgactta taaacacaat
      661 ctctttttcg gaccaattgc tgcagagatt tttgatgttc atcatagaga aaaattccta
      721 caggattgga ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt
      781 ttagttaaaa cattagaaaa aaatgatgta acatcgatta atcgattaaa gcactgggta
      841 tctctaacaa aagaattgtc taatccatct ttgaagaggg aaatagaata ttctcagtca
      901 gaggatttat taaaagcttt aaaatggtcg gaagaagtca atcaacagat tagtcaagca
      961 aaaggattag ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat
     1021 gggaagataa ttattgtaag ctcagctaat aaggaagctg ttcaagaaga atgggaaaga
     1081 catgaattat tatcattagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagat
     1141 attattcgaa gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat
     1201 tctcctggtg atctagaagc tgcaaataaa aataaagttt tcttttatcc tattttggtc
     1261 aataaagaaa aagaatcatg ggaaaactta agaactgatg ttttatttaa atgtttatcg
     1321 ggagattatg tagatattga acaaaaatat atagatactt tttggaaaaa tcttactaat
     1381 aagtagagga gaaagaaaat gactataaaa gtagatttac gaaaaaaacc atataattta
     1441 gatgaagatg ctatttcttg gattgaagag acgattgatt ctatgacttt ggaagaaaaa
     1501 ataggtcaat tgtttgttaa tatgggatct agtcgtgata aaaattactt gagtaatatg
     1561 gtcaatcagt accatattgg agctgtacga tataatccag gaactgctga tgaggtatat
     1621 gatcaaaact atatcttaca aacagaaagt aaaattcctc tattaattgc tgctaatacg
     1681 gaagcaggtg gtaacggagc ttgtacagat ggtactgaag ttggtgtaga agtaaaaatt
     1741 ggtgcaacaa atgatgcgaa atatgcttat gaattaggga gagtttctgg gattgaagca
     1801 tcggctattg gatgtaactg gagttttgct cctattgtag atattaatag aaattggaga
     1861 aatcctatca tttctagtcg atcttttgga caaaaccctg ataaagtttt agaacttagt
     1921 ttagcttata tgaaaggtat tcaggagagt ggaattttgc ctgcagctaa acatttccct
     1981 ggtgacggta ttgacgaacg agatcagcat ctatctttta gtattaatag tttaggtacg
     2041 gaagaatggg ataattcttt tggaaaagta tactcaggct taatagaagc tggattacct
     2101 tcaattatgg caggtcatat ccatatgcca gcttattcaa aacattttaa tcctgatctg
     2161 aaagatgaag atttacttcc tgcaacttta tcaaaagaat taatcactga cttacttaga
     2221 ggaaaactcg gatttaatgg attagtagtt actgatgcta gtcatatgtt aggaatgact
     2281 ggggcaatga aacgatcaga attattgccc acttctattg cagctggatg tgatctattt
     2341 ctatttttta atgatccaga tgaagacttt aattatatga tggaaggcta tcgtaaagga
     2401 attatcactg atgatcggtt attagatgcc ttaaaaagaa ttttagggtt aaaagctaaa
     2461 ttgggattac acaaaaaagc aaaagaacaa attttattac ctaagcaaga agctctggaa
     2521 aaaattggtt taattgaaaa taaatcaatt tttaaagagg tggctgacca agcaattaca
     2581 ttagttaaag ataaacaaga tatttggcct attgtaccag aaaaatataa aaggatttta
     2641 ctagtagatg tacatggggt aaaaggtggg tttggtgaag tagttgctgg aaatagacct
     2701 caagccattg atttattgaa acagattttg tcagcaaaag gatttgaagt tgaaaaatac
     2761 atttcacctg aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa aaatatagct
     2821 ggaatatatg ctgctaagag accaattaaa aatttaacta aaaaatatga cttgatcatt
     2881 aatatagcta atgttaatcc aggcacagtt caacgaattg tctggccagc aagtaaagga
     2941 actccggata taccgtttta cattcatgaa attccaacaa tttttgtatc tgtacagtgt
     3001 ccgtttcatt tggctgatgt acctcaagta aagacttata ttaatactta tgatggaaaa
     3061 caagaaactt tagaaatatt ggtggacaaa ctaatgggtc tttctgaatt taagggagta
     3121 agtccaatag atgcttattg tggtttaaag gatacattta tttaatattt agataggagt
     3181 ggtttatatg ttgtatccca ttatgacaga gacgaggcaa gtaattgact taagcggtct
     3241 ctggagattt aaattagata caaatgatga cttacaggaa gaacttgctt tagaaccaat
     3301 aaatgaaaaa gagagttatc cgatttcagt tccctcttct tataatgatt tatttgaaac
     3361 agagaacatt agaaatcatg ttggatgggt gtggtatgaa aaagaagtga ctcttcctaa
     3421 aagattatta gaagaaagat tagttcttcg ttttggatct gcaacacatg aagcaaaagt
     3481 ttttttgaat ggaaagttta ttactcaaca caaaggtggg tttacaccat ttgagtctga
     3541 aattaatcgg tttgtaaaag acgggaataa tcgaattaca gttgctgtaa acaatattat
     3601 tgacgatagt actttacctg tggggattat gaaggaaaca gtacaggaaa atggggatat
     3661 aaaaaaggaa aacttagtta attttgattt cttcaattat gcaggtattc atagacctgt
     3721 taagttatat acaactccga aagactatat aagtgatatt actattaata caagaataac
     3781 tgatttggat gcaagtgtag aattcaatat cgatacagaa tggaccggaa aaatcttaat
     3841 agcgatatat gacgaagagg ctcaacttgt agctgaagct gaaggaaaga aaggaatgat
     3901 tcagcttaag aatccaattt tttgggaacc tctagacgct tatttatata gtatgaaaat
     3961 tagtttaatt gatgataata aagtgatcga tacctataca gaagaatttg gtattcgcag
     4021 tgttcaggtt accaatggca aatttttgat taataataaa cctttttatt ttaaaggatt
     4081 tggtaagcat gaggattcgt atgttaatgg acgtggtttg aatgaggctg taaatattaa
     4141 agattttaat ttaatgaaat ggataggtgc taattctttt agaacatcac attatcctta
     4201 ctcagaagaa attatgcgat tagcagatcg agaaggtatt gtagttattg atgaagtacc
     4261 tgcagtaggg ttacatttaa atttcatggc cactttatta gatgataatg tggaaaaaca
     4321 taatacgtgg aaagagatac gaacagaaaa ttctcataaa acggtgatca aggaattgat
     4381 agaacgcgac aagaaccacc caagtgttgt aatgtggtct attgcaaatg aaccagattc
     4441 ggattccgaa ggtgctaaag aatattttga acctttagtt gaattaacta aagaacttga
     4501 tccgcagaaa agacctgtaa caatcgttac ttatctaaaa tcaacgccag atgtttgtaa
     4561 ggtaggagat attgtcgatg ttttatgttt aaatcgttat tatggttggt atgtttcggg
     4621 aggggatctg gcacatgcaa aatatctgtt gaaaaaagag ctagatggat ggatggaaag
     4681 atgtcctgat acgccaataa tgtttacaga atatggagca gatacagtag ctggatttca
     4741 tgatacagta ccaacaatgt ttacagaaga gtatcaagtg gcttattacg aagcaaatca
     4801 cgaagtaata gatacatgtg aaaattttgt tggtgaacaa gtgtggaatt ttgcagattt
     4861 tgcaacaagt caagggattt taagagtgca aggaaataag aaaggaatct ttactagaga
     4921 tagaaaacct aaaatgattg cttactatct acagaaacga tggtcatcaa tacccaattt
     4981 cagttataaa aaataatttg gattttttaa gaatgttcaa aaa
//
LOCUS       DADVXN010000146         4980 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.150, whole genome shotgun sequence.
ACCESSION   DADVXN010000146 DADVXN010000000
VERSION     DADVXN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.150"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            45..1186
                     /locus_tag="KB942_002625"
                     /pseudo
     CDS             45..1186
                     /locus_tag="KB942_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1312..2391)
                     /locus_tag="KB942_002626"
     CDS             complement(1312..2391)
                     /locus_tag="KB942_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6853002.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2494..2934)
                     /locus_tag="KB942_002627"
     CDS             complement(2494..2934)
                     /locus_tag="KB942_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6853003.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2991..3401)
                     /locus_tag="KB942_002628"
     CDS             complement(2991..3401)
                     /locus_tag="KB942_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6853004.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3581..4015)
                     /locus_tag="KB942_002629"
     CDS             complement(3581..4015)
                     /locus_tag="KB942_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6853005.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4115..4963)
                     /locus_tag="KB942_002630"
     CDS             complement(4115..4963)
                     /locus_tag="KB942_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6853006.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1812 a          960 c          842 g         1366 t
ORIGIN      
        1 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
       61 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      121 cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      181 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      241 cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag
      301 ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga
      361 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
      421 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc
      481 cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg
      541 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
      601 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
      661 aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa
      721 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
      781 ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga
      841 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
      901 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
      961 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
     1021 taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa
     1081 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1141 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1201 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat
     1261 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     1321 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     1381 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     1441 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     1501 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     1561 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     1621 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     1681 gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg
     1741 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     1801 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     1861 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     1921 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     1981 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2041 ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa gcatcgggat
     2101 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2161 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     2221 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     2281 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     2341 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     2401 gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt
     2461 ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt cttgatgaaa
     2521 attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct gttcaagtgg
     2581 aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg
     2641 agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac gattaatata
     2701 atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca gacacaaatc
     2761 atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca tcacaagata
     2821 ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa tgatttcttt
     2881 agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt ccatttttcc
     2941 acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt ctattcacta
     3001 actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg acacacttta
     3061 tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat agatccttca
     3121 atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac ttgtgcactg
     3181 acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc actgttaaaa
     3241 catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc gacgcgttta
     3301 ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc tccagagcgt
     3361 ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaagttacc acctttaatt
     3421 atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa atggattggc
     3481 ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag caaacatata
     3541 caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat ttctcaaaaa
     3601 gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc cataaataac
     3661 caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct gtcatttctt
     3721 gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata tcagagacga
     3781 tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa ccaagagacg
     3841 tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact tcattattgc
     3901 ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt tgatacagtt
     3961 ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta tgcataaaga
     4021 tctcctctcc ttttcagtcg aacagttact agattctttt attataccag ctaaagaaaa
     4081 cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga taagtaaatc
     4141 cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga tcgatttctt
     4201 ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat aaaacctctt
     4261 ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt ccaatctcag
     4321 cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata acagatttct
     4381 ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca atagataaaa
     4441 tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca acgaatgcgt
     4501 ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa atgaggggag
     4561 ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact cctaaagcaa
     4621 aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt gtttctggaa
     4681 ctattgcaat gattaaagga aaaaccaagc ttccgaaaag tactttaaaa aagggttttt
     4741 tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa ataactatag
     4801 ctcggtcaaa gcctaaagca gcttccagta atattcctag accgctgaca cctccagcag
     4861 caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag agggcaaaaa
     4921 taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttattca cttccttaat
//
LOCUS       DADVXN010000147         4974 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.151, whole genome shotgun sequence.
ACCESSION   DADVXN010000147 DADVXN010000000
VERSION     DADVXN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.151"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(297..1499)
                     /locus_tag="KB942_002631"
     CDS             complement(297..1499)
                     /locus_tag="KB942_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6853007.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(1559..1762)
                     /locus_tag="KB942_002632"
     CDS             complement(1559..1762)
                     /locus_tag="KB942_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBB6853008.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(1904..3136)
                     /locus_tag="KB942_002633"
                     /pseudo
     CDS             complement(1904..3136)
                     /locus_tag="KB942_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            3299..4111
                     /locus_tag="KB942_002634"
     CDS             3299..4111
                     /locus_tag="KB942_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853009.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1522 a         1015 c          775 g         1662 t
ORIGIN      
        1 taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct aatatgataa
       61 ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc cgtttcaacc
      121 acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct ttttattata
      181 gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag gagcaattcg
      241 actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg gaaaactcag
      301 actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt atcaatcgca
      361 cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa agtgatttga
      421 atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg ctcaattaga
      481 aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc cgctttttta
      541 gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc gtccaactga
      601 gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct gagttttaag
      661 gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg agtcgaaaag
      721 taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt gtttttctca
      781 aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc agtataaaag
      841 agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt caattcattc
      901 atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc agaaggaagc
      961 gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc ccgatccaat
     1021 acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt ttcgccatta
     1081 acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca atcctcaata
     1141 tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg cttttcgaaa
     1201 atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca ttttacaaac
     1261 ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg attaggaatt
     1321 gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc atctttttgt
     1381 gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat acctagatac
     1441 gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat ttttggcatc
     1501 gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct tgtactgttt
     1561 attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc acaccaactt
     1621 ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg ctctctttaa
     1681 tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca cgatttacca
     1741 tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt ttaaaataat
     1801 cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct tgtcgaggcg
     1861 gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag cttgttttct
     1921 catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga taatactccg
     1981 ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct ccttgttcaa
     2041 taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata aaaactaatg
     2101 atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga ggaattgtat
     2161 ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg gcatcaactt
     2221 ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta agtaactcaa
     2281 agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt tgcgctttct
     2341 cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa ggaattcctc
     2401 gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga ctcttctttg
     2461 agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta ccgccatgag
     2521 attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc ttaagttctg
     2581 gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta aagttcccct
     2641 gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt tttagccatc
     2701 ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta accgcatcgt
     2761 caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata tagccctttg
     2821 gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct tcaattagct
     2881 ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca cggttaactg
     2941 gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca cttaattgtc
     3001 tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt tctgctaaat
     3061 atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg agatcatgtt
     3121 gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag aacctattga
     3181 acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa tatgttttga
     3241 ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag gagttttcat
     3301 ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag aattaaagtt
     3361 gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag tcaataactg
     3421 ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag agataggtca
     3481 tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt tagaaatgct
     3541 ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag acgaaatcaa
     3601 acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt ttattacaaa
     3661 tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg aaaaaaagca
     3721 gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc aacatgataa
     3781 tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg tggatcgcat
     3841 actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt tacccaagga
     3901 actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg atgtgcgaat
     3961 tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg acaatcaata
     4021 tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga aagaacgcct
     4081 gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc acgtgacaaa
     4141 gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt tctttgttag
     4201 ccccctgtta gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac
     4261 aaaactccag atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat
     4321 tttgaaccct aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc
     4381 attaagtaca gttttcctac ctatttttga taacccgctg gttagttaat attccttttc
     4441 cgagagagat aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga
     4501 aatacttcat cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta
     4561 attgatttca gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc
     4621 ttcccccgat gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat
     4681 atacataatt acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa
     4741 gaaaaacaga aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca
     4801 ataatatttt tctaacttat cgccttcaaa gaaatagttt cttatttttc actaaaataa
     4861 aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca atcatttcaa ggtaatataa
     4921 ctacctgagt ttattatcga ttaaaatgct aactacattt gcaagagaag cgag
//
LOCUS       DADVXN010000148         4931 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.152, whole genome shotgun sequence.
ACCESSION   DADVXN010000148 DADVXN010000000
VERSION     DADVXN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.152"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            83..970
                     /locus_tag="KB942_002635"
     CDS             83..970
                     /locus_tag="KB942_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6853010.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="KB942_002636"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="KB942_002636"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBB6853011.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="KB942_002637"
     CDS             2272..2718
                     /locus_tag="KB942_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6853012.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="KB942_002638"
     CDS             2991..4343
                     /locus_tag="KB942_002638"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBB6853013.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="KB942_002639"
     CDS             4514..4801
                     /locus_tag="KB942_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853014.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DADVXN010000149         4806 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.153, whole genome shotgun sequence.
ACCESSION   DADVXN010000149 DADVXN010000000
VERSION     DADVXN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.153"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB942_002640"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB942_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBB6853015.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="KB942_002641"
     CDS             complement(2066..3064)
                     /locus_tag="KB942_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBB6853016.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="KB942_002642"
     CDS             complement(3290..3943)
                     /locus_tag="KB942_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBB6853017.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB942_002643"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB942_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6853018.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1413 a         1007 c          745 g         1641 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatg
//
LOCUS       DADVXN010000150         4759 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.154, whole genome shotgun sequence.
ACCESSION   DADVXN010000150 DADVXN010000000
VERSION     DADVXN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.154"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            174..518
                     /locus_tag="KB942_002644"
     CDS             174..518
                     /locus_tag="KB942_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBB6853019.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="KB942_002645"
     CDS             519..938
                     /locus_tag="KB942_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBB6853020.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="KB942_002646"
     CDS             complement(1030..1380)
                     /locus_tag="KB942_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853021.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="KB942_002647"
     CDS             complement(1346..2677)
                     /locus_tag="KB942_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBB6853022.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="KB942_002648"
     CDS             2933..3499
                     /locus_tag="KB942_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6853023.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="KB942_002649"
     tRNA            3644..3731
                     /locus_tag="KB942_002649"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..4631
                     /locus_tag="KB942_002650"
                     /pseudo
     CDS             4065..4631
                     /locus_tag="KB942_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT         1739 a          797 c          910 g         1313 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaacgaga gtataaaata ttttgtgtaa atgaaaaaat ccatacaaaa
     4681 aaggaagtcc cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa agaggacttc
     4741 cctatgaatg attttacta
//
LOCUS       DADVXN010000151         4731 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.155, whole genome shotgun sequence.
ACCESSION   DADVXN010000151 DADVXN010000000
VERSION     DADVXN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.155"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..531
                     /locus_tag="KB942_002651"
     CDS             <1..531
                     /locus_tag="KB942_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6853024.1"
                     /translation="IRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLE
                     LNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKK
                     ENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGED
                     AFTNRAKRYGFKQIVE"
     gene            581..970
                     /locus_tag="KB942_002652"
     CDS             581..970
                     /locus_tag="KB942_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6853025.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            957..3302
                     /locus_tag="KB942_002653"
     CDS             957..3302
                     /locus_tag="KB942_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6853026.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            3395..4582
                     /locus_tag="KB942_002654"
     CDS             3395..4582
                     /locus_tag="KB942_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBB6853027.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1521 a          798 c         1022 g         1390 t
ORIGIN      
        1 ataagagtta gtggggaaag tagtattttt tcagataaaa aattaatcaa ttctataaga
       61 aaattgaatg atgcacaagt tcttgaatta caaaccctta agccaaatga tttagtagtt
      121 atccgacttg aactaaatgg aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag
      181 acatctgcaa ttctttctat gattttggcg tatggaaatt caccactcat attggatcag
      241 cctgaagatg atttggatag tcaattaatt aataacttaa tcgtaagaag tatcattaca
      301 aaaaaggaaa acagacaaat tttaattgta actcataatg caaatatccc agttaatggt
      361 gattcagaat ggttgattag catgggagat tcaaaagaaa tatcggctga tcagtgcggg
      421 tcggtagatg aacccaatat caagccaaga atatgtactg tgatggaagg tggcgaggat
      481 gcttttacaa atagagcgaa acgctatggt tttaaacaaa ttgtagaata acactagagg
      541 gtgagacata ttattatgtc cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta
      601 taccagtatc tgggcagttg aaaaagtcat ttacgcaatc aatgattttc agttgccttt
      661 tcctgtgact tttaaccaaa tggcttggtt cgttttatct ttactgtttg tcatcgtatt
      721 tgcccatgtg cctccactat ctatgataga ggaggtcttt ttaaaatact taggcatacc
      781 ggtggctgtc acttggttta tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt
      841 tttacgatcc tttatcagct atcacttgcg atttaaggtg acttttgcag gaaagaaagt
      901 caaagaacag aagaaacgat gggatgaacc cagtactctt gtaaggagtg tgaactatgt
      961 atccgattaa gtatattgaa aataatctcg tctttaacca agaaggcgag tgttttgcct
     1021 actatgaatt agtgccttac aattactcct ttttgtcacc cgaacagaaa tttcaagtgc
     1081 atgataactt tcgccaacta attgcgcaaa atcgtgaagg caagatccat gcgttacaaa
     1141 ttgccacaga aagtagtatt cgcgccacac aagaacgatc aaaaaaagaa attaccggta
     1201 gactcaaaga agtagcgaaa caacgaattg atatacagac agatgcatta gtatccatga
     1261 ttggcgatag tcagattgat tatcgctttt ttattggctt taaactaatt gcgacagatg
     1321 aagaagtcaa tctaaagagt ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct
     1381 atggagtgaa tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgct
     1441 gctattctaa gcttgaaaaa ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc
     1501 gagtcacacc gagtgacctg acgtatttga ttgaacatat ttatggcgaa aaagggatac
     1561 cgtttgaaga atacgaattt cagctaccaa agaaaaagct aaaatcagaa acactggtga
     1621 aacgttatga cttattacgt ccaaatcgtt gtttaattga agaaaaacca aggtgcttac
     1681 ggatggaaca tgagaatcat gagtcgtacg tagcgtattt aacaattaat acaattgtgg
     1741 gggagatgga atttccttcc tcagaacttt tctattacca gcaacaacaa tttacattcc
     1801 caatcgacac aagtatgaat gtagagattg tgacgaataa aaaggcttta gctaccgttc
     1861 gtaataagaa aaaagaactg aaagatttag acaatcatgc ttaccaatct gacaatgaaa
     1921 cgaattccaa tgtcttagat gctttggatt ccgtggatga attggaaacc accttggatc
     1981 agtcaaaaga atctatgtac aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg
     2041 atgaactaaa gcgacgctgt gatgaagtgc tcgattttta tgatgatacc aatgtaaaac
     2101 tagtccggcc ctttggcgat atgatggggc tacatgaaga attcttaccc tcaagtaaac
     2161 gatatatgaa cgactatatt cagtatgtta cgtctgattt tttggcgggg cttggttttg
     2221 gggctactca gatgctagga gaattagaag ggatttactt tggctacaat gtggatacag
     2281 gtcgtaatgt ttacctgaaa ccggcattgg cttcacaagg agtcaagggt tcagtaacca
     2341 atgcgctggc cgccgctttc ctgggttctc ttggtggagg taaatccttt agtaacaatc
     2401 tcttagtcta ctatgctgtt ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg
     2461 agcgaggagg gtggaaagaa accttgccag aaattgcgga tgaaatcagt atcctcaact
     2521 tgacgagtaa gtcagaaaat caaggtctgc ttgatcctta tgtgattatg aagaaaaaga
     2581 aagactcgga aagtttagca attgatattc tgactttttt aacaggaatt tcaagtcgag
     2641 acggggagaa gtttcctgtg ttacgtaaag ccattcgaac ggtgactcaa agtgatcaac
     2701 gggggttatt acaagttatt aacgaattgc gaaaagacgg gtcaccaatc gcggaaaata
     2761 tcgccgatca tatcgagtcc tttaccgatt atgattttgc tcatttgctt ttctcagacg
     2821 gctcgattac ccaatcgatt agtttagata aacagctaaa tatcattcaa gtggcggatt
     2881 tggttttacc ggatgcagaa acgacttttg aagaatatac caccatggaa ttgcttagtg
     2941 tttcgatgct aatcgtcatt tccacttttg ccttggactt tatccatagt aaccgagaaa
     3001 tcttcaaaat tgtcgatttg gatgaggctt ggagttttct tcaagtggct caaggaaaaa
     3061 cgttatcgaa taagcttgtt cgtgctggtc gggccatgaa tgctggggtc tatttcgtca
     3121 ctcaaaatag cgacgattta ttagatgaac gtttaaaaaa taatatcggg ttgaagtttg
     3181 cttttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt ggtttggatc
     3241 aggaggatca aagcaatcaa aaacgcctgc gagatttgga aaacgggatt acgtccatat
     3301 aattgtgtaa aagtgaagca tcaaaaaagc cattgatctc agttaggagt acaatgtttt
     3361 ttgcagaaaa catactcgaa taggactgat atcaatgact caagtacatt ttacttttga
     3421 aagcgaagaa attcaagcaa ttataaacga aagcggtgcg aatgataccg caaaaacact
     3481 tatgactatc atgtttaatc agctgatgga agaacaacga aatcaatata ttcaggcaaa
     3541 tgcctatgaa cgttcagaag aacggcaatc acaaagaaat ggttactacg atcgttcgtt
     3601 tacaactcga attggtacgt tagaacttca tgttccacga accagagacg gtaagtttag
     3661 tcctactatc tttgaaagat atcagcgaag cgaaaaagct ttgatcgctg ccatgattga
     3721 aatggtcatt tctggtgtct ccactcgcaa agtaactaaa acagttgaac tgctaacgga
     3781 tggtgcgact gtttctaaat cattcgtttc aaatctgatg aaacagttgg atccatttgt
     3841 ttttgagtgg agaaaccgga gcctagaagg ctcggaatat ccatttttca tgtgtgatgc
     3901 cctgtatatg aaagtaaggg aaaatcatcg tattgtctca aaaggtgtat atattggtat
     3961 cggcattgat tctgatggac gacgtacgat tcttggtttt gatgttcaag acggcgaatc
     4021 ggaagataat tgggataccg tttttcaatc gttcgttcaa cgaggtttgt tcggtgtaaa
     4081 actggtcatt tctgatgcac acaaagggtt agttaaggct gttcgcaaga actttttagg
     4141 tgcgagctgg caaagatgcc aagcccactt tttgagaaat atttttgata agctgccaaa
     4201 gaaagtttct tctgatgtaa aagacgagtt gaagagtatc tttaaagcct ctgaacttga
     4261 attgacacgt gaaagaaaag agcacttctt ggaaaaatat ggttgtgatt caaaattaag
     4321 tgctgcttgc gacattttag aaaacggctt tgaagatgct attcaaattt tgtcttttcc
     4381 tgaaaatatc cgccgaagga ttcgtacaac caatgtgttg gaacggttga acgaagagat
     4441 tcgccggaga gaacgagtga ttcggatttt tccaaatatc aattccatca ctcggataat
     4501 tggaacactt ttgatggaaa aagacactga gtggctggct tctccacgaa aatatttaga
     4561 atttaattcg aataacattt agcaccatga acctgtgcga gtctcttctt tctgaggaga
     4621 gacatcaaga gtcgctgcgc tactgctcct gacctctctc ctcagaaaga aaggcatggc
     4681 agttaggttc acaacgcaaa acattgtgct tttgatgagg ttgaactttt a
//
LOCUS       DADVXN010000152         4620 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.157, whole genome shotgun sequence.
ACCESSION   DADVXN010000152 DADVXN010000000
VERSION     DADVXN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.157"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            153..959
                     /locus_tag="KB942_002655"
     CDS             153..959
                     /locus_tag="KB942_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6853028.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="KB942_002656"
     CDS             977..1684
                     /locus_tag="KB942_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6853029.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="KB942_002657"
     CDS             1681..2652
                     /locus_tag="KB942_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6853030.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="KB942_002658"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="KB942_002658"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6853031.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="KB942_002659"
     CDS             3679..4287
                     /locus_tag="KB942_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6853032.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="KB942_002660"
     CDS             complement(4353..4526)
                     /locus_tag="KB942_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853033.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          972 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DADVXN010000153         4466 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.158, whole genome shotgun sequence.
ACCESSION   DADVXN010000153 DADVXN010000000
VERSION     DADVXN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.158"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            61..2730
                     /locus_tag="KB942_002661"
     CDS             61..2730
                     /locus_tag="KB942_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBB6853034.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2821..3441)
                     /locus_tag="KB942_002662"
     CDS             complement(2821..3441)
                     /locus_tag="KB942_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6853035.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3555..4094
                     /locus_tag="KB942_002663"
     CDS             3555..4094
                     /locus_tag="KB942_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853036.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4091..4429
                     /locus_tag="KB942_002664"
     CDS             4091..4429
                     /locus_tag="KB942_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBB6853037.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1388 a          777 c          842 g         1459 t
ORIGIN      
        1 agaaaaacaa ggatatgata aactgaatac aacttcatag aaatttggtg gaaaataaat
       61 atgaaaaaaa atttaaagtc attctttaga gaaaacggac gctacgcagc agctagtttc
      121 ctgattccgt ttttgatttt agcacttgtt tatctaagta tcgggatcta tcctggaagc
      181 agcagaagta ttttggcaag tgatgccttt tcacagtttt ctaattttca tgccagcttc
      241 cgaaatgttc ttcttggaaa gcaaagtatt ttttatacat ggaatgcttc tctaggattg
      301 aattatttat ctttaatttc ttactatcta ggcgggatct tcacgccact ggtgttcttt
      361 tttccaaacc agatgatgcc agatgcactg tatgttctga ctttgcttaa ggtcggctgt
      421 gccggattgg ctttttggtt tcttgcacgt acgttcaaga ttcctcgctg ggggcatgtt
      481 gcacttagcg tcagttacgc attgatctca tttatcactg cacattcgga gatcatcatg
      541 tggctggatg catttgttta tcttccatta gtgattttgg ggattcatcg tgttatggat
      601 cttaaacggc cgaaactttt atttatcagt tatttactct tgtttttatc cagtttttat
      661 atgggtttta tgattggcgt attttctttc ttatatttta tcgcccgtct attgacaaac
      721 tggtcagaat ataaaaaaac gattgttcct tatggtatca cttctttact ggcaggcggc
      781 gcttcaatga tcattgtatt gccagcagtg ctggatcttc ggtcaaatgg cgaggcactg
      841 agtcagatca ctacgttgaa aacagaagcg acttcttatc tggatctaat catgaaaaat
      901 atgattggtg tttatgacac gacaaaatac ggatcgattc cgttcgttta cgcaggtctg
      961 ctaccattag tcctctgtct gttttatttt gccagtcgga aagtaccgtt gaaaaataaa
     1021 tttttgtacg gcagtctttt cgccatactt atcgcaagtt tctatcttgt tccattgaat
     1081 ttgttctggc atggtatgca tgcaccgaat atgtttttat tccgttatgc ctatctgttc
     1141 tctttcttag tgctcctgct tgcaggaaaa ggatgggaaa agatagaaca agacgatcgt
     1201 gggctgttgt taggaatcat tttggtcgta gctgctctct ttgcggtggc atggggcatc
     1261 aaacctgaag gaagctacac ctatgtgacg atgacttcct ttgtattgac agtggttttc
     1321 ttagggattt atgcttttgc tgtgggattt tatcagcata aaaaactttc attcaatcat
     1381 ctgagtatat tgttattatt gatcatgtct gctgaagcac tggtcaatac gaacagtatg
     1441 atccatggca ttcttgatga ttggaattat gcttcccgca gtttatatac tgatccttat
     1501 cctgacttga aagctcttgt aaatgaaaca aaaaaagatt ccgactcgtt ttaccgcttg
     1561 gaaaatctgg atccagtatc tgcaaacgat tcgatcaact atggttacag tggtatcagt
     1621 ttgttttctt ctataagaaa tcgtaattct tcctcttacc tggataaatt aggtttccgt
     1681 tcaagaggga caaatttgaa tatccgttat aacaataata ctttgctaat ggatgggttt
     1741 acaggaatca aatataacat ttctaaaaac gaaagcacgt tcagtaaata tggcttccgc
     1801 caagctggtc aaagtggcga ctatacactg tatcagaatg tcaatgcatt gccactagca
     1861 tttactgcac cgttgtccgt taatgatgtt gagcagccag tgacagataa tttgaccagt
     1921 cagacaaact tgatcaatac attgtctggt ttaaatgaac agtattttac attctataca
     1981 cctactttga agaatcagtt gaatacgacg atcacaaaga caacgactgg cgtcacttac
     2041 tcagaaacag cagataacca gccaaaagag ctcacttggg aagtccatgt gcctgcaaat
     2101 acacaagctt acttaagttt gttcccaacc aattttgcac aattagaaag ttctagtgcg
     2161 acgatcactg tcaatggtac atcgcgaaaa acgcaaatca acatttctgg tcaatactat
     2221 gatcttggct attatcctca agagacaaca gtgacattta ctgcaagttt ttatggaacg
     2281 aaagaagtaa gtttcatgga gcctaaagtc gttggtctag atgttgtagc ctatcaagcc
     2341 gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag ccactggcag aaaagcttct
     2401 gggactgtaa ataccgctga agattccatg ttggtgacaa ctattcctta tgacaaagga
     2461 tggaaagcta aaattgatgg gaaaccagtc aaggtcagca atttcaaaga cgcattcttg
     2521 atgataaaag ttccagcagg taaacatact gtcactttca gttacttgcc tgaaggattt
     2581 acgataggtg caatcctttt tgtcctatgt attgcattgt ttattcttta tgtctggtac
     2641 cttgaccgca atcgcccctt ttatggcacg attgatccag caatagaaga aaaaccgcga
     2701 aatacacaca gaagaagacg aagaaaataa agaaacgatg gaatctgtgt cactcttcta
     2761 tttaaatggc aaaaatttca atctttaaat gacaaaaacc acctgatttt tggtggtttt
     2821 ttatatacct atgactaaac tatctcttgc tgcttcgaca acttctacag tgatcttatc
     2881 catttgattg attcttaaaa tccgatcaat ctgcgcaaat aaacgatgaa taagcctaaa
     2941 atttcctttt gtgatcttaa tgatagttgt gatagcctca taatcagaaa aatcttctag
     3001 cttgatatca attcctaaat ctgcccattt gtattccaaa atatgatgtg tttcgtcctt
     3061 actaaggtta tcaaattcat gtgcaaatcc tatgcgagag taaagctgcg gataacgaga
     3121 caatcttttt tcaataccag gcataccgat aagaaccatt gccagatcat ttctatcgta
     3181 aatatctctt agctgttcta aatgctgaag ctttaaacga tcaatttcat cgacaataat
     3241 taagtcaatt gcttcatagg ttttaggcac aggaggatct tcttgattgt attttagtac
     3301 catatgcatt gctttagtca gacctattct actagttatt gtgttaatat ccgaactgac
     3361 acgagacatc ttttcagctg gtacagtata aaaaacttta tttacactta atatttcatc
     3421 tgttgcattt atcaagaaca agaaaaagtc tgaatattca aaaaaaataa cggtgtacat
     3481 ttttaaaagg agatgtataa tcgagattta gaaagcgttt tcctcaaaat ttaatgaatt
     3541 aggaagtgat tactatgaaa aaattattta gcgcactttt tattctgtta tttggcttta
     3601 ttggtattgg gacttctgga aatatagtta aagctagcac gatggttgaa caaacaagtg
     3661 aaatggcaaa aacttttagt caattaacac cagaagaaca aagttacttc aaatccaaag
     3721 gatttagtga taaagatact ttttacactc aaaagataga gcctcaagat ccgaaaacac
     3781 gtttttctgc aaatgtattg cggttaaccg catctactaa aaaaattact aatacgcagg
     3841 ggcggacaga atatattata acaagtccca attcggcgat gtatcgtatt tctacacgct
     3901 tgagtttagg tagtgcaaaa tcatatactc aaacagtaaa tctagctaat tcttatggtt
     3961 atggtggagg aatgtatttt acatttactg ggaaaagaca atattacagt gtaaaattag
     4021 ctatgcaagt ttataataca tttggtgtag ggtcagtctc ttgcactgcg ggaggattga
     4081 caataggaaa atgataattt cttactatgt cttaaataga agtgatgatg aaggactctt
     4141 cgtttttcaa tcattacatg aggatacgat gctaaaagag ttttataatc ggattaagaa
     4201 taaacagata gaaacaaatt tatatttttt aagtgactct atcttgcctt ttattagcaa
     4261 ccctaaaatt cttaacttgt tgcagacaaa agatttgaaa agtttaccga ttattgtcat
     4321 tgatgaggaa attgtaaaat atggcgctta tctaagcgta gaagaattag aaaaattaac
     4381 taacattgga atgagtattc aggaggataa tggggtacat ggaacataga atgaatatgc
     4441 acagttgaga acggaatttg acattt
//
LOCUS       DADVXN010000154         4421 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.159, whole genome shotgun sequence.
ACCESSION   DADVXN010000154 DADVXN010000000
VERSION     DADVXN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.159"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            93..974
                     /locus_tag="KB942_002665"
     CDS             93..974
                     /locus_tag="KB942_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6853038.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            987..2597
                     /locus_tag="KB942_002666"
     CDS             987..2597
                     /locus_tag="KB942_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBB6853039.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2674..3333)
                     /locus_tag="KB942_002667"
     CDS             complement(2674..3333)
                     /locus_tag="KB942_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6853040.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3616..4281)
                     /locus_tag="KB942_002668"
     CDS             complement(3616..4281)
                     /locus_tag="KB942_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBB6853041.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1193 a          857 c          872 g         1499 t
ORIGIN      
        1 taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag
       61 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
      121 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
      181 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
      241 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
      301 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
      361 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
      421 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
      481 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
      541 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
      601 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
      661 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg
      721 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
      781 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
      841 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
      901 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
      961 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
     1021 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
     1081 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
     1141 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
     1201 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
     1261 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
     1321 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
     1381 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
     1441 tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
     1501 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
     1561 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
     1621 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
     1681 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
     1741 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
     1801 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
     1861 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
     1921 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
     1981 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
     2041 tagtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
     2101 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
     2161 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
     2221 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
     2281 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
     2341 gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact
     2401 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
     2461 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
     2521 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
     2581 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
     2641 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
     2701 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
     2761 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
     2821 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agagagcgac
     2881 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
     2941 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
     3001 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
     3061 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
     3121 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
     3181 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
     3241 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
     3301 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
     3361 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
     3421 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
     3481 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
     3541 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
     3601 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
     3661 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
     3721 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
     3781 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
     3841 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
     3901 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
     3961 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
     4021 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
     4081 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
     4141 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
     4201 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
     4261 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
     4321 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
     4381 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt a
//
LOCUS       DADVXN010000155         4391 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.160, whole genome shotgun sequence.
ACCESSION   DADVXN010000155 DADVXN010000000
VERSION     DADVXN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.160"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            55..1356
                     /locus_tag="KB942_002669"
     CDS             55..1356
                     /locus_tag="KB942_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6853042.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1383..1643
                     /locus_tag="KB942_002670"
     CDS             1383..1643
                     /locus_tag="KB942_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6853043.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2642..3877)
                     /locus_tag="KB942_002671"
     CDS             complement(2642..3877)
                     /locus_tag="KB942_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6853044.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1281 a          696 c          898 g         1516 t
ORIGIN      
        1 tttgcgttgt catgcaatga atggttcata ttttattgaa aaggagtggt tttagtggaa
       61 aagctgatga aatgggtcga agagaaactt gtcccaccaa tggcaaaaat tggtacacag
      121 cgtcatttat tagcaattcg taatggggtt gtttcaacat tgtcattgat tttgattggg
      181 acatttttta tggtctttat caatttaccg tttcctggat ggaatgaatt tattgcccct
      241 tattcggcaa cgattgtttt accctttagg atgacgatgg gattgatggc tatttatgca
      301 acttttgtta tgggttcgga tctagctaaa tcatatggat tagatagtgt gacaggtggt
      361 atattatctt taggaacgtt ctttatgtta caagttcctg taaacgtttt aactcctgaa
      421 gaggcacctc ttggctgggt cttacctatg agttctttgg gagcatctgg gatgtttgcg
      481 gggatcttat ctatgatttt tgcagtggaa gtatatcgtt tctttaaaaa tcgaaatatt
      541 actatcaaaa tgccagaaca agtgccacct gctgttgccc gtagttttga ggcattgatt
      601 ccaggtgcag tcattttaac aattacttgg ctgattcgaa gcgtcgcggg ctttgatgta
      661 aacgctgcct tattatcgtt attcgagcct ttaaccaata ttttagggaa taatttgctt
      721 ggtgttttac taccaatgtt tttgatccat ttgttgtggt catttggtat ccatggcatg
      781 agtattgtag gggctgttgt tcgtccaatg tggctgatca tgttggatga gaatgcaaag
      841 gcattggcag atggtacagc tgccacccaa ttaccctata tcgctcctga acagttttat
      901 caatggacgg taacaattgg tggtgcagga gcaacgatcg tggtatctgt tttgtttata
      961 ttcttctgta aatcaaaatt cttgaaagag gttggtcgtt tttctattat tcctggtatc
     1021 ttcaatatca atgagcccat aatttttgga gctcctatga tgttgaatcc ttacatgttc
     1081 atcccgttca acttagttcc tttagttttg actattatca gttattttgc agtcaaatta
     1141 gaaatggtca atagatttac tgttttgcca acatggacat taccagctcc aatcggcggt
     1201 tatctttcag caggaaatga ctggcgtgtt gttatcttga tcatgatcaa cacattgata
     1261 gcatttatta tctattatcc atttgtaaaa gcttatgata aaaagatgct gtcagatgaa
     1321 caaagtcgtg gagaaaacga agtccagact gtctaagttt ggtttttaga aggtgggaaa
     1381 caatgtattg gctgatttta ttttttgttt ttatcttttt attaacggct agtcacctta
     1441 tattgaatat gctagctaca tatcatattc aaatcaatcg atggatttgg gcactggcat
     1501 catttttgat cgtgattctt ccaaagatca ttgtaccgca tatgaatgtt ttgttttcat
     1561 gggggacata tgtactttgt ggaatttttg ctatcaattt tatgatcgag cagcatagat
     1621 ggtttgtcac atcgaagctt taaatgttct aaaagtggtt gtaacagaag tggttagctt
     1681 caagaaataa gaagaaattt acgaaaattg cttcttaaat ttttgtgaaa tgttggctta
     1741 tttttcgttt ggttgaactt attaccgcag atgcaagctt ttgaccaccg tttattcgga
     1801 atatgggact gtgacaaggc ttttgtcaca gccctttttt acataaagat tattattgtt
     1861 ttaactgttt tggcagaaag aaggaggaaa aaatggaaat tttatcagca atggatttag
     1921 aattagtaga tctactacca aagtgatcaa gtcaaatata gatttgggag cttacaacca
     1981 tatagtaggg gctgcagtat ggtgtatgaa tggtaatatg tatgatgtga gtgtttattc
     2041 aaatcatgaa acgtgtatag aaataattgt gctgggaata gcaatcagtc atggtgaacg
     2101 tgacttttag tgtatcgttg ctgacgacat agaacattct gacatgatct actcaatatg
     2161 tggggattgc tggcagttta tataggagta tgcatcggat tgcgagatca ttgtctcaac
     2221 agaagaggga ggttgattat caaagataaa gtggtaaatc ctacatttga tttagtaatt
     2281 gttgagggga ctagcggcac aaatctctac aaagcgatgc ttctagatct atatgacaca
     2341 ttttaagaaa atgtatttgc ctaattatta tggtctttct tttgaagaaa cgttgagatg
     2401 atccaaccag agaaaggtgt caaagaattt gactaagaat aaatggcaga cgctacctat
     2461 gctcatttgg atagcgtcag atcttttatt ttaaggatga ttttgtgttt tttagacacc
     2521 gtaatttaag taactctaac ttttcaatcc actatttact ttttttgtat tttcactgtc
     2581 tgggtctcga atagcgaaat tactgatgag tctctattaa aaaagctttt tctacttgaa
     2641 actatattgg gtggttgctc aggaaggttt cgatttcttg aattgcttgt tgtggaccat
     2701 ttattggaaa aacggcttgc agcgtgcgtg cctgctgacg aatcttgggt gaagaaactt
     2761 cgcgcagagc aatctgcagt tttctttcag tcactggttt tgtgagggcg attgcgtttc
     2821 cgtattttac acaatagtca atattacagc tttgctcaaa ttgcatcccc acaccgataa
     2881 acggtttgcc ggattcacag gcagtttgga cggtcccttc cccgccgtgg ataaaagaaa
     2941 aatcaatgaa ttcatgcaaa agatgactag acagatattc tgttattaaa agatcttctg
     3001 agtcagattg aatgtcatca agcagatagt caataggtgc aataaccgct agatccggtt
     3061 gtttttgtaa gaagaacaaa attttgcgta acatacgcgg attgcctgaa ctacccattg
     3121 cgaaatagat aatacgtttt ctttcttgtt gctttcgctg tataaaagtt agcacttctt
     3181 caggcaagga agtactgagc ttagcaaaaa ttggaccaac gattcgataa ttttccggca
     3241 gctgttcttc ttccaaaaat agtttaggcg ttgtgatgag atttagatca gcatccatca
     3301 tttgtaaact tgtttttggt aaaggaagca tttctttttg tgcagcgtgt ttgaaggcat
     3361 tggggatcca tcgggtattt actatacgct tttttgtcca gtgccaaagg ggattgctta
     3421 aagggccgaa gaaacgtaac tcgtggggca tcagcgcatg ctctgattga aaaaaaggtt
     3481 cactcaaagc atagggttta acatatacta aaggaatctt tttattgcgg gccgataaaa
     3541 aaattgtcac attcgaacct gtcacaatca gtcgaggctc aaacttagca atcagactct
     3601 gttcactagt gatacgagag gcaaccattt ctttggtaaa agggttttta agtgtacgca
     3661 actggtcaaa ttttatgatt tgttcttctt gttttttcgt aaggatcggc gctagtaaat
     3721 gatactcaaa gccactttct gtgatcaaag gagcaaaagt tttagaaaag ccaaagaata
     3781 cacagtaata acggtctttc atagctttgg caacctcaat cattcgtgtt gtctcagcta
     3841 aattgaatac tgcaggagca aaaagaattg tcttcatgaa atcctcctta ttggaatgag
     3901 tctatccctt cgtttcttac tagtatacta aaaaacacag cgttcgagta gcctatgtat
     3961 attttgaata ttaaactgtc tctcgctgtt ttcacaactt ctaaatttaa attttgtaat
     4021 caagttgaaa ctccaatttt caaaaaaaca cgcaacaatt ttttgatcga tttatatgtt
     4081 gtgccagata aatacgagaa gttgtgtcat taaaaataaa catgagaaca taaaaaaata
     4141 aaataattat attaattgat aggtgtgctg tcgtcattat acacacgtaa gaaagaaata
     4201 gttatgttta aaaatattct tagactttct cgccatgaca attttcacta tgtgttataa
     4261 ctgtaaacga taattattca gataacacaa aaagaaatca aggagcagat acgccagttg
     4321 tttaatttga atattatttt aaaattaaaa tttttattct gttctaaaca tttttggttg
     4381 tttagaactt t
//
LOCUS       DADVXN010000156         4378 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.161, whole genome shotgun sequence.
ACCESSION   DADVXN010000156 DADVXN010000000
VERSION     DADVXN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.161"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(36..932)
                     /locus_tag="KB942_002672"
     CDS             complement(36..932)
                     /locus_tag="KB942_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6853045.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(925..1605)
                     /locus_tag="KB942_002673"
     CDS             complement(925..1605)
                     /locus_tag="KB942_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6853046.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1606..2388)
                     /locus_tag="KB942_002674"
     CDS             complement(1606..2388)
                     /locus_tag="KB942_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6853047.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2381..3298)
                     /locus_tag="KB942_002675"
     CDS             complement(2381..3298)
                     /locus_tag="KB942_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6853048.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3462..3785)
                     /locus_tag="KB942_002676"
     CDS             complement(3462..3785)
                     /locus_tag="KB942_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853049.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1286 a          951 c          761 g         1380 t
ORIGIN      
        1 acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag
       61 atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat
      121 ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg
      181 gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg
      241 gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct
      301 gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct
      361 gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa
      421 aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg
      481 agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata
      541 ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta
      601 tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga
      661 tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt
      721 cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc
      781 ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg
      841 attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag
      901 ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg
      961 aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac
     1021 tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga
     1081 aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa
     1141 atggacagcc acaccattca catttacatc cctcgactct ttgtccagta agatttcctt
     1201 gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat
     1261 gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc
     1321 tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg
     1381 aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat
     1441 gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc
     1501 tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac
     1561 agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga
     1621 tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta gactgaccag
     1681 aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca
     1741 cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg
     1801 aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct
     1861 agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac
     1921 aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc
     1981 gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt
     2041 gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga
     2101 taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc
     2161 tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc
     2221 gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca
     2281 ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt
     2341 acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc
     2401 cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag
     2461 gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa
     2521 gctggataac acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg
     2581 ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca
     2641 attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg
     2701 cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt
     2761 ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg
     2821 tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac
     2881 gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca
     2941 tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac
     3001 gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca
     3061 tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa
     3121 tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca ttttccccaa
     3181 tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag
     3241 caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac
     3301 ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag
     3361 aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata
     3421 cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc
     3481 ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga
     3541 tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc
     3601 agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca
     3661 aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt
     3721 cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa
     3781 aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat
     3841 tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac
     3901 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa
     3961 tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg
     4021 gataaataat attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga
     4081 tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc
     4141 aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc
     4201 ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac
     4261 tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
     4321 gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt tgattaaa
//
LOCUS       DADVXN010000157         4262 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.162, whole genome shotgun sequence.
ACCESSION   DADVXN010000157 DADVXN010000000
VERSION     DADVXN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.162"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(146..1912)
                     /locus_tag="KB942_002677"
     CDS             complement(146..1912)
                     /locus_tag="KB942_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6853050.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1872..3626)
                     /locus_tag="KB942_002678"
     CDS             complement(1872..3626)
                     /locus_tag="KB942_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6853051.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3736..>4262)
                     /locus_tag="KB942_002679"
     CDS             complement(3736..>4262)
                     /locus_tag="KB942_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBB6853052.1"
                     /translation="SLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPR
                     KGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALM
                     QLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMI
                     GVLIGRKVLKKYKK"
BASE COUNT         1443 a          805 c          600 g         1414 t
ORIGIN      
        1 taagaataga cattaagtga actaattcaa tcatactgat tctttcgtgt attcaaaatt
       61 agatgctgtt taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt
      121 gtaggttaat ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt
      181 tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact
      241 cattatgagt accttgatca actagttttc cttgatccat cactacaatt ttatttacct
      301 tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat
      361 ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta
      421 aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc
      481 ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt
      541 caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt
      601 gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa
      661 aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat
      721 caattaaaat acagccttca tcataatcat acagtctaga cactaatttc aagatactag
      781 ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag
      841 agatatgatc taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa
      901 tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc
      961 taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat
     1021 cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca
     1081 cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg
     1141 agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc
     1201 tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc
     1261 tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttc tctctcaaag
     1321 tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa
     1381 aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca
     1441 tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca
     1501 tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct gataaattat
     1561 gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc actcttaaat
     1621 gcgcactttc tttataagta ctattatata agttttcata ctccctatct aacaaccaat
     1681 acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc tttgtatttt
     1741 ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt
     1801 atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga tcacttaaag
     1861 caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa
     1921 ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg atttccacgt
     1981 tctactattt tcccttctga caagacaaga atttcatcta cagatttaat gctggataat
     2041 cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt
     2101 tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca
     2161 tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact
     2221 cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa
     2281 ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa
     2341 acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg
     2401 ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc
     2461 ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat
     2521 tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca
     2581 cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt
     2641 gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt
     2701 tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa
     2761 acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga
     2821 gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa
     2881 aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac
     2941 ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat
     3001 atctttataa cttgaattcc gcggatatat tctaccgttt cagagcttaa cacatctaac
     3061 gattcttgat atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca
     3121 ccaattacta acatggcgat tagaaccaat ccaattttaa agttaatgaa aaagcctaag
     3181 gcaactgata aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt
     3241 gccatatgtg ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt
     3301 aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta
     3361 gtctctaagc gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct
     3421 cctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct
     3481 acaataatta gagactgaag aaatttgtaa atactgtaat atccatagac tgttaatacg
     3541 acagaaaaat cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa
     3601 ctaaataatt ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat
     3661 tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagataa
     3721 aaggtaaact acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc
     3781 aatcatagat aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata
     3841 gtggatcatt gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa
     3901 acgatctgta aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag
     3961 agcccaagac gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc
     4021 agctaagatc gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa
     4081 cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt
     4141 atagctaata taaacaaata tgattccaga taataatcca aaaatcccgg gggatatgct
     4201 gtgaccaaac gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga
     4261 aa
//
LOCUS       DADVXN010000158         4243 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.163, whole genome shotgun sequence.
ACCESSION   DADVXN010000158 DADVXN010000000
VERSION     DADVXN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.163"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            75..503
                     /locus_tag="KB942_002680"
     CDS             75..503
                     /locus_tag="KB942_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6853053.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="KB942_002681"
     CDS             490..732
                     /locus_tag="KB942_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853054.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="KB942_002682"
     CDS             complement(1032..1247)
                     /locus_tag="KB942_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853055.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="KB942_002683"
     CDS             1354..1668
                     /locus_tag="KB942_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853056.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="KB942_002684"
     CDS             1772..2950
                     /locus_tag="KB942_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6853057.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="KB942_002685"
     CDS             3353..3646
                     /locus_tag="KB942_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBB6853058.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1450 a          638 c          820 g         1335 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccc
//
LOCUS       DADVXN010000159         4139 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.164, whole genome shotgun sequence.
ACCESSION   DADVXN010000159 DADVXN010000000
VERSION     DADVXN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.164"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            99..632
                     /locus_tag="KB942_002686"
     CDS             99..632
                     /locus_tag="KB942_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853059.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(758..1435)
                     /gene="nth"
                     /locus_tag="KB942_002687"
     CDS             complement(758..1435)
                     /gene="nth"
                     /locus_tag="KB942_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBB6853060.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1442..2140)
                     /locus_tag="KB942_002688"
     CDS             complement(1442..2140)
                     /locus_tag="KB942_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6853061.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2408..3592
                     /locus_tag="KB942_002689"
     CDS             2408..3592
                     /locus_tag="KB942_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBB6853062.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3598..4041)
                     /locus_tag="KB942_002690"
     CDS             complement(3598..4041)
                     /locus_tag="KB942_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6853063.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1099 a          801 c          665 g         1574 t
ORIGIN      
        1 acttgtttat aattaaatcg gctaaataca cactagtctt ttatttagtg ccacttctta
       61 ataatcccat tgttattatc aaataaaaaa tacaatatat ggttttgaat aattttttga
      121 aagaaggctt cattttgagt tatctgatag tatgcttaat tatcataatt tgtctgcacc
      181 atcttttttt atcgaaaaca atacctcgtt attccaagaa taaaaaagct gaaaaatttt
      241 gtttattatt aaataaattt actttaattg ctcctatcct tgcatttatt attttttctg
      301 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
      361 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
      421 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
      481 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
      541 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
      601 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      661 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      721 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      781 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      841 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      901 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      961 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
     1021 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
     1081 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
     1141 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
     1201 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
     1261 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
     1321 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
     1381 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
     1441 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
     1501 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1561 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1621 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1681 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1741 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1801 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1861 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1921 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1981 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     2041 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     2101 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     2161 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     2221 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     2281 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     2341 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     2401 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     2461 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     2521 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2581 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2641 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2701 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2761 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2821 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2881 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2941 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     3001 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     3061 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     3121 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     3181 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     3241 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     3301 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     3361 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     3421 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     3481 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3541 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3601 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3661 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3721 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3781 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3841 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3901 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3961 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     4021 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     4081 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaa
//
LOCUS       DADVXN010000160         4081 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.165, whole genome shotgun sequence.
ACCESSION   DADVXN010000160 DADVXN010000000
VERSION     DADVXN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.165"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(190..1695)
                     /locus_tag="KB942_002691"
     CDS             complement(190..1695)
                     /locus_tag="KB942_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6853064.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1692..2378)
                     /locus_tag="KB942_002692"
     CDS             complement(1692..2378)
                     /locus_tag="KB942_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6853065.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2571..3992)
                     /gene="gndA"
                     /locus_tag="KB942_002693"
     CDS             complement(2571..3992)
                     /gene="gndA"
                     /locus_tag="KB942_002693"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBB6853066.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1125 a          861 c          696 g         1399 t
ORIGIN      
        1 aaacacgaat attacagcat tttattgttt tttcgttcat ctaaaagata cttacattta
       61 attaacggat gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat
      121 ttctcacttg cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa
      181 gcacatgctt tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc
      241 ttgccctact acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc
      301 gatagacaac cccagaccat ttccaccctt cgttctagct cttgctttat ccactcgata
      361 aaaacgattg aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat
      421 cgcgatctca aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt
      481 tgaatacttt actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata
      541 aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata
      601 taatatcttg aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt
      661 cgcataatga acatctactt gttctgctct tgataagtct agcatctctt gaactaagct
      721 tttcatacgg ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc
      781 atctttcccc caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct
      841 taactcatga gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg
      901 gtcaagcatc tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc
      961 aggcatgtgg atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac
     1021 cttgagtggt tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa
     1081 agaaacaact tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga
     1141 agataattca tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat
     1201 aggctggata gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg
     1261 tgtagtctga atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa
     1321 atcataaaca taaagatcca ttgatggctg ccctagctca gctataaagg gatcgatctt
     1381 cattaagttt ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc
     1441 tttcgtcaga ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg
     1501 agaagctact tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt
     1561 gatagatgat ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga
     1621 actcgcaaag gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt
     1681 tttcggcttt atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt
     1741 tcctccccgg gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt
     1801 gtttcaactt ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc
     1861 acgttcacgt tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt
     1921 acttcatttc cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta
     1981 atagtagttt gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca
     2041 cgaagacgtg ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg
     2101 tggtcaagtc cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt
     2161 gtatttttga cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc
     2221 agatccaaca atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg
     2281 ttgtagtgga cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc
     2341 aagttctttt catcttcaat gattagaata ttgctcattt attattttat cctttctatt
     2401 ctactggctt tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca
     2461 ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca
     2521 gctctttcgt cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca
     2581 tggtaccatg agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca
     2641 aagtaatcgc gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg
     2701 aagtaagcga tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca
     2761 acgacgtctc tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc
     2821 aataaatttt cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga
     2881 gctcggatga tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa
     2941 tcaaattctt ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta
     3001 ctgaaataca acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca
     3061 aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa
     3121 atatagcggg caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg
     3181 ctttggcttg tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact
     3241 actggtttac ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc
     3301 aaatagctgt ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact
     3361 gaaaggttta aaatattctt cattaagtca taagattcag caatcagttg catatctcca
     3421 tactcaatac cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat
     3481 gtgacacatg gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact
     3541 agcgcatagg cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct
     3601 tcaccaccgg aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta
     3661 cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca
     3721 aggtgaggaa gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc
     3781 aagatccgac gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg
     3841 aaatttttat ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat
     3901 aaagccactg aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc
     3961 ataccgacaa cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct
     4021 ccctaatctc ttacattata gcttaacacg gctacattgc aaactaaaga acaacatatt
     4081 t
//
LOCUS       DADVXN010000161         4070 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.166, whole genome shotgun sequence.
ACCESSION   DADVXN010000161 DADVXN010000000
VERSION     DADVXN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.166"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            86..1435
                     /locus_tag="KB942_002694"
     CDS             86..1435
                     /locus_tag="KB942_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6853067.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="KB942_002695"
     CDS             complement(1563..2153)
                     /locus_tag="KB942_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6853068.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="KB942_002696"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="KB942_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6853069.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DADVXN010000162         4067 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.167, whole genome shotgun sequence.
ACCESSION   DADVXN010000162 DADVXN010000000
VERSION     DADVXN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.167"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..1157)
                     /locus_tag="KB942_002697"
     CDS             complement(<1..1157)
                     /locus_tag="KB942_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6853070.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="KB942_002698"
     CDS             complement(1160..3409)
                     /locus_tag="KB942_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6853071.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..3961)
                     /locus_tag="KB942_002699"
                     /pseudo
     CDS             complement(3506..3961)
                     /locus_tag="KB942_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DADVXN010000163         4065 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.168, whole genome shotgun sequence.
ACCESSION   DADVXN010000163 DADVXN010000000
VERSION     DADVXN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.168"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(505..870)
                     /locus_tag="KB942_002700"
     CDS             complement(505..870)
                     /locus_tag="KB942_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853072.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(946..1191)
                     /locus_tag="KB942_002701"
     CDS             complement(946..1191)
                     /locus_tag="KB942_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853073.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1261..1515)
                     /locus_tag="KB942_002702"
     CDS             complement(1261..1515)
                     /locus_tag="KB942_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853074.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1679..2002)
                     /locus_tag="KB942_002703"
     CDS             complement(1679..2002)
                     /locus_tag="KB942_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853075.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2189..2262
                     /locus_tag="KB942_002704"
     tRNA            2189..2262
                     /locus_tag="KB942_002704"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2223..2225,aa:Ile,seq:cat)
     gene            complement(2647..3144)
                     /locus_tag="KB942_002705"
     CDS             complement(2647..3144)
                     /locus_tag="KB942_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6853076.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3184..3993)
                     /locus_tag="KB942_002706"
     CDS             complement(3184..3993)
                     /locus_tag="KB942_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6853077.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1362 a          720 c          714 g         1269 t
ORIGIN      
        1 gggaacctta tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt
       61 tgttctgact ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag
      121 gagttaaaat gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga
      181 atattaggca gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc
      241 aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac
      301 gtcgaagatg cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt
      361 ggcgatgaac caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct
      421 aaaacttacg acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg
      481 agatagaagg cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc
      541 taaacattct gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac
      601 agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc
      661 tggtgctggg ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa
      721 taatctataa ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc
      781 atttcctctt aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt
      841 tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta
      901 accttagacg ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct
      961 tgctgtttct gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata
     1021 gattaattca atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa
     1081 aggaatactt aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa
     1141 attcagacga caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc
     1201 tttcattata gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac
     1261 tcaatgtttt ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag
     1321 tcctgataat tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt
     1381 ccacataaag tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca
     1441 attcataaga taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct
     1501 caccaacgta atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta
     1561 ttgcatacat cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg
     1621 aaaaagcaga acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct
     1681 atcgagaaaa aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga
     1741 tcgcattcac taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa
     1801 atacttttgt ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa
     1861 gcaaaagggt aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat
     1921 aatttaattg gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg
     1981 gcaaaataat cactcctaac attgagaata aataaaagaa taacattttt tctaaaataa
     2041 gcaaataaaa attaaagtaa caaaatagtt atgacacaag taaaagaaat aagaataaca
     2101 atattatttt acttatattt tgaaacttgt gagagtttgt cattcgtgct atagttgaaa
     2161 agtaacaaaa ataaatttta tttatgatgt acctttagct cagttggtta gagcagacgg
     2221 ctcataaccg tccggtcgta ggttcgagtc ctacaaggta cataagaggt tgtgaaaaag
     2281 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttacttg
     2341 gctgttctta ttactgcaga tgcaagcttt tgaataccgt ttaaggaaga ggttgtgaaa
     2401 tcagcgtttt gacctgagta ttagcttaac aaacggaaaa atagttcctc atattttgcc
     2461 gtttgttaag ctaataccga gggtcagctg attgaacacc atttaagaaa gaggtcgtga
     2521 gcctgccgtt tcactctgtg tcataaaaaa gccaatctac tttgatagaa aaagcacgta
     2581 ggactaaaac agttctgcgt gcttttttta ttgtgtgtta aatatcgtaa atgaagatag
     2641 atatttttat gattcgacga tagagacgtt gaaatctttc aacagctcta aagcggattc
     2701 tccgtgtttt ctactggaga ctaacattgg atcaacaaaa aagtctgctt caggaaaagc
     2761 actctgcagg cctatcagat tagagagtac acacaagttt gtttcaatcc cgactacttc
     2821 gatctctttt tgttcttctt tgctttcaaa taaagtttcc tggatttcag ctaaagtttc
     2881 aggtggaatc gtaaagtaat actttttaat cactcgttca ttttcttgtg gagaaagaag
     2941 agggatcaat tgcaaatctt ctcgttcttc ttctttgtcc ttcagttcaa ctggtatatc
     3001 ccgtgtgaag agaatatatt cattattttg gcgtgcttta gctaatcttt gagcaatacg
     3061 ttctgccaat tgatctgact ctggaatata aaattcgctg tttggatcaa gaatatgatt
     3121 ttgcatatca ataataacta acattttctt tcctcctaca ttcatatcgt cattttattc
     3181 tttttaatag aagtttttta aaatataagc aacaccagct tcttcattcg tcagtgttac
     3241 ttggtttgca atttctttta cttgtcgctg tgcattcccc atcgcaatac tcaacccgct
     3301 gtaattcaac atagcaatgt cgttttcttg atcgccaaaa gccatgatgt cactcgattc
     3361 gattccaagg taatccgcca gaaaaacaag tgaatgagct ttagtgatac ctgtaggcgt
     3421 gatttctaag aaatgcggat cagagaatac tgattggata ccatctgctt gcagatctct
     3481 ttgtagcttc tgtagttttt cttcactctt atctgttaat gctaatttaa gatagctaag
     3541 agaaggatct tccaacatat ttaaaatttg atcaaaagaa tggttctgta aattgaaatg
     3601 ttcataataa gggtcatctt tgactagatt tgattcatcg aatgtcagct gatcatcttg
     3661 ggtttctcgg ctagcaatga tggatatttt cgcttcttga gcatagaaga aggcttttct
     3721 cagtgtatcg ctatctattt ttgaacgtct gagaactttc gccatttttc catccttcat
     3781 ttcagcaatc agtgaaccat tggaagaaat caaatagcca ccgattccta attcggaaaa
     3841 aatatcttta gcagaaatgc tgttccttcc ggtagcgacg gttacgattc cgccattttc
     3901 atcaaatttc cgaagtgctt ggatattttc ttctggtatc tttccatttc gatcaagcaa
     3961 ggtaccgtct aaatcaatcg ctgctagttt cattttatct tcctactttc tttttacata
     4021 tctattttcc tgttacctta agattaaatg agaatttaaa aaaat
//
LOCUS       DADVXN010000164         4045 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.169, whole genome shotgun sequence.
ACCESSION   DADVXN010000164 DADVXN010000000
VERSION     DADVXN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.169"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..651
                     /locus_tag="KB942_002707"
     CDS             <1..651
                     /locus_tag="KB942_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBB6853078.1"
                     /translation="KILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIV
                     FEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKI
                     EHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRK
                     ISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            668..1102
                     /locus_tag="KB942_002708"
     CDS             668..1102
                     /locus_tag="KB942_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853079.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1719..>4045
                     /gene="esp"
                     /locus_tag="KB942_002709"
     CDS             1719..>4045
                     /gene="esp"
                     /locus_tag="KB942_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBB6853080.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1482 a          563 c          772 g         1228 t
ORIGIN      
        1 aaaatactgg aatatgttaa aaatggagat attcgaaatt tagaaaacat ggtttttaat
       61 ctgagtaatg gaataattcc aagtgttagt ggagatacga ttcggtctga aaaaaattat
      121 tctatcattg tttttgaaaa gttagcacaa acgtctataa cattaggaat ggatataata
      181 gaagcgtatc aaagtagaga tgctttgata caagaaaacg aattagctgt atcgttacct
      241 gaggtcttaa aagtgaggga ttctgggatt gtttattata ctaaggaaat cggaaaaact
      301 aagatagagc atctttctcc cttaatttct tctgtcgttc aattcatcgg cttaaatatc
      361 tataaaagaa ttactgtaaa ggaaatagct aattattttt ctgtaagtga aactaaatta
      421 agagaaagct ttaagaatga aatgcatatt acgatttata attatatctc taagcgaaaa
      481 atttctacgg ctaaaataat gttgaaatca aaccatacta tcagtgaagt ttctttaggt
      541 ttaggatttt cagattcttc acacttttcg agagtattca aaaagtacgc aggtgtatct
      601 cctaagcaat atcaattagg tttagtggac aatttcaaca acattagctg agaggtttta
      661 ttttattatg agactatatc atttacgtag tttggaggag ttgaggaaat tggattttaa
      721 caaaaaattt ttttgggaaa tttctgattt gagttgtttc gtagaatctg agcgtctaag
      781 taatggtaat aatagtgtgt taaatgaata tgacaaagta gtaattaata aagtaaaaag
      841 tttatgtgaa gaagctagag aatctatcag tcatattaat ggtagcaatt ttaaacaaac
      901 attatttaat ctgattaagc tagatgctaa aatatcaagt tatttattct ttttgataca
      961 tgatgaattt atggattatc aaaaattgga tcagttaatt gaaagagaaa gctgggaaga
     1021 ttattacgtg ggtctaactt tttcagaaaa gttaaacaac tacaagttaa tagattataa
     1081 gtatttgagt gaaagcaatt aattattcat gtagagtact agacacttta aatatggaca
     1141 attcaattat aaagaaaaag aatcaataat ttaatgaggc tgtgacaaat gtcatttttt
     1201 gtttcgctaa aaacgagtga aaaaatgttg ataactccaa tcattcgttt aagattctag
     1261 gaatcattca gaaatgctgt gaaaccgata ctttataatt ataaagaagg tgatttcaaa
     1321 ttccgaagaa ggaatttgaa gtcacctttt tacttatgtc acagtctcat tcagtaatgc
     1381 gggataaggc tccttattcc gttatttgaa tactctgaag tttaatctaa gaaagggtaa
     1441 tattttgaaa acatgcttca tcgcatagaa atgaacttaa tttcagaaag aatacataca
     1501 tatcttttta tatgttttca gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac
     1561 atgaagaaat gtactttttt tgttgtaatg ttcatgaaat tctctaagta aattagttaa
     1621 cataaggtta gtaagattcc tattctgtga ataatacgta tcagatttat cttttattct
     1681 tactaggaaa gtaattatta agggagttga tttgaataat ggttagcaag aataataaga
     1741 gagtatttct agaaaaaaca aagaaacgag tattgaagta cagtatcaaa aaattaagtg
     1801 ttggggttgc atctgttttg attggtgttg gtcttgtctt gggaacaact gaactagtta
     1861 aagcacaaga tgaaatttct ccaagcagcc cacttgaaac agcgataagc tccgtacaag
     1921 taggtgacaa agtagcttcg gggaatactt ttcaggagaa tcctggttat acgaaaaatt
     1981 ataacttttc ggatttacaa tttagtcctc aagaactaac tggagacaca ctaaaaggca
     2041 atacaattgg atttgaagtt tacggaaaac ataatattgc agcatcaact aaaaactggg
     2101 aaatccgtct tcaattagat gaacgattag ctaaatatgt agaaaaaatt caggtcgatc
     2161 caaagaaagg aattggatct tctagacgga cctttgtaag aattaatgat tcgctgggta
     2221 gacctacaaa tatttggaag gtcaattata ttcgtgcaag tgatggatta tttgctgggg
     2281 cagaaacaac cgatacgcaa accgctccta atggggtaat tacgtttgaa aagagcctag
     2341 atgaaatttt taaagagata ggcatagaca atctcaagac cgatcgttta atgtatcgaa
     2401 tctacttggt aagtcatcaa gacgatgata aaattgtacc gggtattgat agtacgggct
     2461 actttttaac agattcagat gatttctata atagcttaga tgtatctgaa aataatcctg
     2521 accagtttaa acatggatca gtaagtgcaa aatatgagaa acctaatact caaacaaaag
     2581 atggttcagg atccacaggt gctaatggag ctataatctt agatcataag ttaacgaaaa
     2641 attataattt ctcatattca tcttccgcaa agggcacgcc ttggtatgct aattacaaaa
     2701 ttgatgaacg attagttcca tatgtagcgg gtatacagat gcacatggtt caggctgaca
     2761 aagtaacata tgatgtttcg tttgaatccg gaacaaaagt agctgattta gcaatcgagc
     2821 gacgggaagg tcatgaaaat tatggcatag gttcaataac taataatgat ttaactaaac
     2881 ttatcgattt tgctaatgca agtccacgtc cagtcgttat tcgatatgtt ttacaattga
     2941 ctaagccatt agatgaaatc ttagaagata tgaaagcaac agcacaagtt gaagaaaata
     3001 aaccatttgg tgaagatttc atctttgatt cttggttgtc ggatacgaat aaaaaattaa
     3061 tccagaacac ttatggaaca ggttattatt atttgcaaga tattgatggt gatggaaacc
     3121 ctgacgataa agaagagagc ggagacacga atccatatat cgggaaacct gaattagaag
     3181 aagtatatga tgttgacaca acagttaagg ggaaagtatt catccacgag ttagcgggaa
     3241 caggtcacaa agcccaactt gttgataaag aaggtactgt attagcagaa aaaactatcg
     3301 ctccaaatga aaaagatggg gctccaattt cagatactgt agaatttgaa tttacgggtg
     3361 tagattcaag taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt
     3421 ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg gttgataaac
     3481 aaaccgtggt agttggattt aaaccagatg ctaaagaatc aattcggaat aataaaaact
     3541 tacctgaaga tgcagagtat tcatggaaaa cagagcctga tacttctaac gttactgata
     3601 gtacgaaagg tattgtaact gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt
     3661 ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac atacgtacct ataacaacaa
     3721 ctccagaaac gacagttcaa agtggaaaac ctacatttga taaaccagat gttcctttag
     3781 ctaaagatgc cttttcaatt tcagatgtct ataataagta cttcggtaat gcaagtgttg
     3841 atgcaaacac tggtattgtt acattcactc cagctaaagg tgtaggagaa tcggagccga
     3901 ttactggaac aattcctatt aaaattgttt accaagatgg ttctgtaggc acgaccgatt
     3961 tagcagtaac tgtaagtaaa gatatttatg aaaatccagg agaaaacatt cctgcaggct
     4021 accacaaagt aaccttcacc gcagg
//
LOCUS       DADVXN010000165         4033 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.170, whole genome shotgun sequence.
ACCESSION   DADVXN010000165 DADVXN010000000
VERSION     DADVXN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.170"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..555
                     /locus_tag="KB942_002710"
     CDS             <1..555
                     /locus_tag="KB942_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HBB6853081.1"
                     /translation="EKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPN
                     SDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIP
                     VINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRR
                     ITKTLNQILNDGEDIKWIQKQIAY"
     gene            528..1205
                     /locus_tag="KB942_002711"
     CDS             528..1205
                     /locus_tag="KB942_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6853082.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1195..2337
                     /locus_tag="KB942_002712"
     CDS             1195..2337
                     /locus_tag="KB942_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB6853083.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2350..3327
                     /locus_tag="KB942_002713"
     CDS             2350..3327
                     /locus_tag="KB942_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6853084.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3327..>4033
                     /locus_tag="KB942_002714"
     CDS             3327..>4033
                     /locus_tag="KB942_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6853085.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFT"
BASE COUNT         1518 a          496 c          747 g         1272 t
ORIGIN      
        1 gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaccgaact agaaaatata
       61 gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc aattatttgc
      121 attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca gaaatattta
      181 aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac tatattaaaa
      241 gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc atcattcaaa
      301 ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa aaatattata
      361 aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca agatcctcag
      421 tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga
      481 ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa
      541 caaatagctt attaaaatat actagtgatc catacattat tattggggct ggcggacacg
      601 caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg
      661 ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct
      721 gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa
      781 gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc
      841 caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg
      901 ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg
      961 ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg
     1021 gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga
     1081 agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct
     1141 cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat
     1201 tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag
     1261 gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt
     1321 attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc
     1381 atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca
     1441 tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt
     1501 gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa
     1561 gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg
     1621 gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat
     1681 gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt
     1741 gggatctatt catttaacgg taataagatc atcacaacct caggtggagg agctctagtg
     1801 tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa
     1861 gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc
     1921 ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt
     1981 ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg
     2041 gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt
     2101 gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg
     2161 tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat
     2221 aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat
     2281 atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg
     2341 tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga
     2401 ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga
     2461 cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa
     2521 aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataaccca tgaatttatt
     2581 ataaaagagg ctttaaattt agattgtaat atctttacgg aaatcaattt attaaatgaa
     2641 tattatgatg aaataatcga agtagctaaa aagaaagata gattacttta tctatcttct
     2701 acatttttga agagaaaaga aattcaatat attaagtcta aagtgacgca taataaaaaa
     2761 atttcttatc aataccatgt aggacagtat ttacccgact ggcatccttg ggaagattat
     2821 cgcaattttt ttgtttcaaa caaaaaaact aatgggtgta gagaaatatt cgctattgaa
     2881 ttaccttgga ttatagatac atttgggaaa gttaaatcgt ttactgtaat gacccagaaa
     2941 ttatccaatc ttgatattga ttattcggac agtttcactc ttattattga gcatgaaact
     3001 ggtatagttg gaactttaag tgtagatgtt gtttcaagag tagctaaaag aaatttaact
     3061 ataattggtg aaaagatttt aatagaatgg gaaggtaacc cggattctct attttcatgg
     3121 aatgaagaaa cttctagtat gacagatatt caattgtacg atgaggttat gcatgataat
     3181 aattattcac atactattat tgaagatgca tatttagagg agattaaaga atttttcacg
     3241 cttttggaaa agaaaatgga accccaatat acttttgaaa aagataagta tattattaac
     3301 ttaattaacg acattgaggt aaattaatga tgaaagtaat tttttttggg ttagggtcaa
     3361 ttggaaagag acatctaaaa aatctaataa gctatggaaa aaagaattct attaattttg
     3421 atataacagc ttatagtagc agtggaaaaa aagagtttga agaaataacg tatattcaaa
     3481 atagagacga tttggataca cattatgata tagcttttat tacaaatccg acttttaagc
     3541 atctggaaac actaaatgta attgttgaaa aagttgatta catattttta gaaaaacctg
     3601 ctttttctga gagtattgat cttactcaat ataattatga ttacaaaaat atttatgttg
     3661 ctgctccact gaggtataaa aatatcatgg aagttgttcg taatagtatt caaagtaaaa
     3721 aagtatattc tgtaagagct atttgttcta cttatcttcc tgattggaga aaaggtactg
     3781 attatagaaa taattattct gcctttaaat ctatgggggg aggagtagaa ttagactgta
     3841 tacatgaatt ggattatatt ataaacctat ttggtttccc taaatcagta aaaaaacagt
     3901 ttggtaaata ttcgaatctt gaaattaaca gtaatgattt agcaacatat atattgagat
     3961 acgatgataa aattatagaa attcatttag attattttgg aagagttcct acaagaaaat
     4021 ttcaattgtt tac
//
LOCUS       DADVXN010000166         3876 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.172, whole genome shotgun sequence.
ACCESSION   DADVXN010000166 DADVXN010000000
VERSION     DADVXN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.172"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(716..3865)
                     /locus_tag="KB942_002715"
     CDS             complement(716..3865)
                     /locus_tag="KB942_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6853086.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DADVXN010000167         3768 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.173, whole genome shotgun sequence.
ACCESSION   DADVXN010000167 DADVXN010000000
VERSION     DADVXN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.173"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(83..334)
                     /locus_tag="KB942_002716"
     CDS             complement(83..334)
                     /locus_tag="KB942_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853087.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            complement(426..683)
                     /locus_tag="KB942_002717"
     CDS             complement(426..683)
                     /locus_tag="KB942_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853088.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(695..1456)
                     /locus_tag="KB942_002718"
     CDS             complement(695..1456)
                     /locus_tag="KB942_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBB6853089.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            complement(1480..1704)
                     /locus_tag="KB942_002719"
     CDS             complement(1480..1704)
                     /locus_tag="KB942_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBB6853090.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            1864..2610
                     /locus_tag="KB942_002720"
     CDS             1864..2610
                     /locus_tag="KB942_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853091.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            2686..2892
                     /locus_tag="KB942_002721"
     CDS             2686..2892
                     /locus_tag="KB942_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBB6853092.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            2995..3648
                     /locus_tag="KB942_002722"
     CDS             2995..3648
                     /locus_tag="KB942_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853093.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
BASE COUNT         1202 a          711 c          659 g         1196 t
ORIGIN      
        1 accatgtaaa cgatcgtgcc gattaatgct tgtctgcttc cccaaagtcc taaagcatag
       61 acgattaggg gtgcgctgaa cactagtgct ctgttgaatt tacccatgtt tttcctcctt
      121 ttagatactg atacaaagcg atactactaa acctccactc tctgccaact ttcgctgctg
      181 gaatctttcc agattcggca tctttagtca gcgtgcgtgt tgtggttttt aaatattccg
      241 cagcttgctt tgtatcccac acttcatttg caatctcaga ttctgccaaa gaagctttaa
      301 ggtctgagag gttaactaaa gctagttgct ccattggtta tttcctcctg tatttataat
      361 tcgtacattg caataatcga atctataatg atatagattc ggatgaaact gtcatctata
      421 aaacatcagc tgttaaaagt atcggtttca aatagtaatt cttgctcgtt gatagcggaa
      481 gccaactttt cttccaacac atcggcgagc tttctttttg ttggattcat ttcaaatttg
      541 atagtcttga aaagcatttt tgcatcccaa actttttgtc cttgcaattc cttgatgatt
      601 ttatcggctt ggctatcaat tgcatgtgtt agatcatcta tcattttttg taccttgatt
      661 gtttggctca tttcctcgtt catagtgtta cctcctatgc tgtttgttca cgttcaatca
      721 tcggtaagta attattcttc ttcaatgttt catataagaa gatgtgtcct ttttgcgtcc
      781 atttcatcct tgtttttact tgccgattgc cgtttttatc ttcataatca aatggctcaa
      841 tatgcgtgta tcctttgtct tggaacttac tgtatagcag ccatgtgcca ctttgattga
      901 actggatacc taattcatgt agcaatttat taaacgatac agcgctcata ccgtagttct
      961 tagcaatcgt actaactgta actaatcctt tgttgttcaa gattcggtcg taatagtctg
     1021 ccttcggttg taattcattt actcgttgtt cagctatcaa tcgtaacgtg cgttcttctt
     1081 tgagtttggt tgctacttca atcagcaagt ctggattgtt aagtagttca tctgttgcgt
     1141 acatgccgtg ttttcggatt tgaggtagga cttccgttgc taaccagtct tggaattttt
     1201 cagcaagtac gttgtttgct ttaaaggcta atttgtaaac cattggctca ctgataaagt
     1261 cgtttttcgc aacttcttgc gaaaggtatt tttttaaata tccattgaca gttctccacc
     1321 gaacatattc tttaccgttt tttgtttcca cgaaacccaa acttcttgct actgtttcag
     1381 cattaaataa cgattcacca ttttccgtct taacttctaa ttcaaataga ttgttttcaa
     1441 atttttgtgt gttcattttt tcgaattcct ttcttaagtt cagacatttt gaacttctaa
     1501 attaaaaaaa tattcgccaa tttcttcttt tggaatatct agcaattcaa tggctttttg
     1561 aagttcggtg cttttccatg gtactcggtt attcaacttt aatgacaaac ttctttccga
     1621 caaacctaaa gcgattgaaa aattgtattg cgttccatat ttctcaacaa ttcttcccga
     1681 caacttagaa taatcaaaac tcatattagt tcgctccttt ctagttcaaa tgttttgaac
     1741 tttgtaacca aagtataact cctgcataaa acattgtcaa cataaaagtt caaattaatt
     1801 taactttttt attgaactta tgttcaataa ggtatataat aagtatcaga aaggaggtat
     1861 aagatgaagc aaacgactca gcaacgactt aatcagttaa tgtctgaaag aaatttaaag
     1921 caagttgata ttttgaatat gtctttacct ttgcaaaaag aaactggaat aaaaatgtca
     1981 aaaagtcatc tatcgcaata tgtcaatggt aagtcatcgc ccgaccaaca caagctttat
     2041 ttgttggcta aaactttaaa tgtgagtgag gcatggttgc ttggttacga tgttcctaaa
     2101 gaagataaga aaaataacat tccatccatt gaaacaatct acaatcaatt agaaaaacca
     2161 agacaaacaa aagtctacaa ctttgctgaa cagcaactcg aagagcaaaa tcgaggaaac
     2221 gtggtgcact ttcctaaaaa ggaaaaactt ccgacaatca aaaattcagc ttctgccgcc
     2281 aacccgactg aattagttta tggcgataca gttgtagagg aagaagaatt tgaaagagtt
     2341 ccaaacaatg ctgattttgc tgttcctatt ataggcgatt caatggaacc agttatcagg
     2401 aatggacaat ttgtatttgt taaggaacag ccagacgtgg aagacggtga gattgctatc
     2461 gttgaaatag atggtgacgg tgtgacgtgt aaagaagtat ataaggatta tgagagtcaa
     2521 acgattattc tacgctctat aaacgaatta tatgaagata gagtcgtctc accggaacag
     2581 attagaataa tcgggaaagt cgtattctaa aaaatacccc agtcgtagtt ggcgctccgg
     2641 ctaggggtta gtatttatta tcatagtaga aagaaggaaa agaatatgcc aagttacgtt
     2701 gtattgcaag ttgtattaaa agaaaagttt ataggaaaag ggtcgcaaaa cctatcagaa
     2761 cttgaaaaca ctataaatag acagtgttct aaaggttatc gattacacac tatttccact
     2821 acaaacggtg gtagtaaagg tttcggtggt ggcgatagaa tccaagctac tttagttttc
     2881 gaaagtctat aaacaaaaaa acacgcccac cgaccaaagt ttgtggacgt gggatgttaa
     2941 acctatagta ggcttcttta caacttattg tatcaaagaa aagaggagta taaaatgaaa
     3001 aaagttagcg ttatgttgtt gttaagtact gctctgctac tttcagcttg ttcaaataat
     3061 aaaaaagctg aatcaacaga tgccacttct aaccaagaaa caaaaataag taaaacaaaa
     3121 gaaacaactg aaaccagttc atctactagc aaatctacat ctaaaacaga ttctagttca
     3181 acagttacaa gctccaacca agttacggcg gaacctagcc caacagttat aagctccagt
     3241 cagagtacaa tccaaaccgc acctcaagaa gaaacatatg aacagatgaa acaacgcact
     3301 ttacagtcaa ctccagctga tcgtgcaaat tggtccaaca aagagtggga agctttcggc
     3361 gtggcccttt atgaaaatgg attgactaca gatgatgctg gcaatattat cagtcaagat
     3421 cagaaagaac aacaagcagc atctcaacaa aatccagaag accaacaaac aagcgctcag
     3481 caagacgctg acactttatc acttactgat tttgttaaca aatacgggat gtcgcctgtt
     3541 gcatggaaag tacagaatgg aatgtctgaa gaagaagcat tgcgtacaac acagcaaaag
     3601 acttccggtg aagttcaatt aggatttttt aaatacggaa ttcaataata tatttttatg
     3661 ccctactatt ttgcctataa tctctaaaaa agttataaat aaaaaagccc gtgctgcaac
     3721 acggactcat acctcatttc tgagatcaca aatatattat aacaagaa
//
LOCUS       DADVXN010000168         3721 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.174, whole genome shotgun sequence.
ACCESSION   DADVXN010000168 DADVXN010000000
VERSION     DADVXN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.174"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            54..506
                     /locus_tag="KB942_002723"
     CDS             54..506
                     /locus_tag="KB942_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853094.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="KB942_002724"
     CDS             520..1209
                     /locus_tag="KB942_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853095.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="KB942_002725"
     CDS             1334..3685
                     /locus_tag="KB942_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBB6853096.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1263 a          485 c          773 g         1200 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 t
//
LOCUS       DADVXN010000169         3721 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.175, whole genome shotgun sequence.
ACCESSION   DADVXN010000169 DADVXN010000000
VERSION     DADVXN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.175"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..356)
                     /locus_tag="KB942_002726"
     CDS             complement(<1..356)
                     /locus_tag="KB942_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853097.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="KB942_002727"
     CDS             complement(616..1164)
                     /locus_tag="KB942_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853098.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="KB942_002728"
     CDS             complement(1165..2022)
                     /locus_tag="KB942_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6853099.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="KB942_002729"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="KB942_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="KB942_002730"
     CDS             complement(2584..2913)
                     /locus_tag="KB942_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6853100.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="KB942_002731"
     CDS             complement(2990..3262)
                     /locus_tag="KB942_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6853101.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="KB942_002732"
     CDS             complement(3262..3525)
                     /locus_tag="KB942_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6853102.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1402 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3721 a
//
LOCUS       DADVXN010000170         3687 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.176, whole genome shotgun sequence.
ACCESSION   DADVXN010000170 DADVXN010000000
VERSION     DADVXN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.176"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            48..800
                     /locus_tag="KB942_002733"
     CDS             48..800
                     /locus_tag="KB942_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6853103.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            797..1711
                     /gene="pfkB"
                     /locus_tag="KB942_002734"
     CDS             797..1711
                     /gene="pfkB"
                     /locus_tag="KB942_002734"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6853104.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1729..3660
                     /locus_tag="KB942_002735"
     CDS             1729..3660
                     /locus_tag="KB942_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6853105.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1188 a          661 c          845 g          993 t
ORIGIN      
        1 tagtagaaaa aaaatatgct attatttgta ttgaggtgat cgtgaaaatg cttacagagg
       61 aacgcagaca aaaaatccta gacttgcttg aacagcagaa aatcgtgaaa tcacaagatc
      121 ttgtaaacct tttgaatgct tctgaatcaa cgataagacg tgatttacaa gagctggaga
      181 atagtgggtt tctagaaaga gttcatggcg gagctaagaa agagcaatta ttgaattttg
      241 agcagaatat gagtgaaaaa tcactcaaaa acgttcacga aaaacagcaa atcgccaaat
      301 cggcagctga aaccattaat gacgaagatg tcatttattt agacgctggc tctactacgt
      361 tagaaatgat tccttttctg caaggaaaac agatcacagt agtgacaaat tccgttcaac
      421 atgcggcaaa tttagttgat ttggagcttg ctaccattat tttaggtgga acgataaagc
      481 tggcaactaa tgctgtttta ggatctaatg cgatagaaca actaaaaaat tttcatttta
      541 ataaagcatt tatgggaatg aacggtgccc atcttgaaca aggattcacg actccggatc
      601 cagaagaggc agctgttaaa cgtttagcca tgaaaaattc cgaagagaca tttgtattga
      661 ttgatcatac caaattcaac cagttgtcct tcacctctgt tgcacctttg gaagcagcca
      721 cgatcataac cgagcgttgt ccattagaat tcgttaatga attccgtgaa aagacaacaa
      781 ttaaggaggc aatacaatga tttacacagt aacacttaat ccatcaattg attatatcgt
      841 acgcgtagat gggttaaaac taggcgattt gaaccgaatg aaggaagact tcaagttacc
      901 tggaggaaaa gggatcaatg tttcccgtat tttgaaacgg atcgattctc aatctacagc
      961 actagggttt ttaggagggt tcactggcga atttatttcg aagtggcttg aaaaagaaca
     1021 aatccagtct ttgttcacaa ccgtaaaaga agatacaaga atcaatatca aactaaaatc
     1081 agacactgaa actgagatca atggcttagg cccatctatt tcagatgaag aaatcgatga
     1141 gttgaagcaa gcattaggaa cagtaggaca aggagatatc gtcgtattgt caggaagcgc
     1201 gccagctagc ctgcgaaaag gattttatca agaactgatt gatatcattc gagaaaaagg
     1261 tgcagatttc gtgattgata caacaggtga tgacctaaaa aatgcacttg ttaagaaacc
     1321 gttgttgatc aaaccaaaca atcacgaatt agctgagctt tacaacacaa ccttccaatc
     1381 tgtagaagaa atcattccgt atgggaaaaa attactagct gaaggggcac agaatgtgct
     1441 gatctcaatg gctggagacg gtgctttgct cttcacggaa caaggaactt ataaaacgaa
     1501 tgtattagtt cgcccattga aaaattcagt aggtgcaggg gattcaatga ttgctggctt
     1561 tattggggct tacagccgta cgaaagatcc aatcgaagcc tttaaatggg gagttgcttg
     1621 cggaagcgca acagcttttt ctgatgactt ggcagctcgc gaattgattg atgagttgct
     1681 gccagaagtt gaaatcacaa aaattgatta gtttcaagga ggataatcat gaatatcaag
     1741 gatttaatgg tcaaagaagc gatgatcatg gatttgcaag cgactgataa aaaaggcgca
     1801 atcgatgaaa tggtccaaaa aatgtttgat gccggccgga tttccgatat cgaaacatat
     1861 aaagaaggaa ttctagcccg cgaagcacaa acttctacag gtcttggtga tggaatcgcc
     1921 atgcctcacg cgaaaaacag tgcagttaaa gaagcaacag ttttattcgc aaaaagccaa
     1981 aaaggagtag attatgaagc cttagacgga cagccaactt atctgttttt catgatcgca
     2041 gctccagaag gcgcaaacga cacacatttg caagcattag cagcactatc tcgattgctg
     2101 attgatgcag atttcgtagg aaaattaaaa gaagcaaata caccagaaga agtccaagac
     2161 ttgttccaaa cagctgaaga gcaaaaagaa gcagaagaaa aagcagaaca agaaacacaa
     2221 gctgcagaag cttctgctca tccagaaaga aaattcgttg ttgccgttac agcttgtcca
     2281 acagggatcg ctcacactta catggcagaa gatgctttga agaaaaaagc gaaagaaatg
     2341 ggtgtggaga tcaaagtcga aacgaacggt tcagaaggaa tcaaaaaccg attgactgct
     2401 gaagacatcg cccgtgcaga aggtgtaatc gttgcagcag acaaaaaagt agagatgaat
     2461 cgctttgacg gaaaagaact gatcaatcgt cctgtcagtg atggtatccg taaaccagaa
     2521 gagttgatca atctagccct tagtggtact gcaccagtat tccatggtga tggaaaagaa
     2581 agcagttcag aagaaggaaa tgcggatggt actatcggac aaagaattta caaagatttg
     2641 atgaacggcg tatcacacat gttacctttc gttattggtg gaggaatcgc tattgcgctt
     2701 tctttcatga tcgaccaatt tattggcgtg ccacatgatc aattatcaaa tctagggaat
     2761 tacaatcaag ctgctagctg gttcaaccaa atcggacaag ctgcatttgg tttcatgctg
     2821 ccggtattag caggatttat tgcttcaagt atcggtgacc gccctggttt gatcgttgga
     2881 tttgctgctg gtgcattagc taatacaggc ggtgcaggat tcttaggtgc actgattggt
     2941 ggattcttag ctggttatgt catcgtattt ttacgtaaat tattcaagaa cttaccaaaa
     3001 tcattggatg gaattaaaac gatcctcttt tatccagtgt ttggcttact gattactggt
     3061 ttcttgatgt tgctggtcaa tgtccctatg aaagcaatca atgatgcatt gaacggtttc
     3121 ttgacaagta tgagtggttc gaatgctgct ttacttggtg cattattagc tggaatgatg
     3181 gcggcagatt taggcggtcc aatcaacaaa gccgcttatg tgtttggtac agcaacttta
     3241 gctacgactg ttgcaactgg cggaagtgtc gtgatggctt ctgttatggc tggaggaatg
     3301 gtacctccat tggcaatttt cgttgctaca cgtttgttta agaataaatt cagcaaaaca
     3361 gatcaagatg ctggtttgac gaacatcgtg atgggactat cgtttgtcac ggaaggatcg
     3421 attccatttg cagcagctga cccgattcgt gccattccaa gttttattat tggttctgct
     3481 ttaactggcg gattagtcgg agctttcgga atcaaattac tggctcctca cggcgggatt
     3541 ttcgttgtat tcttactgag tcacccggtc atgtatttgg tgtttattgc tatcggagca
     3601 gtcgtatctg gtgtgattta tggtttattg agaaaagctc ctgcagtagc agaagcatag
     3661 aaataaaaaa gaagaggctg ggttttc
//
LOCUS       DADVXN010000171         3666 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.177, whole genome shotgun sequence.
ACCESSION   DADVXN010000171 DADVXN010000000
VERSION     DADVXN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.177"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            11..478
                     /locus_tag="KB942_002736"
     CDS             11..478
                     /locus_tag="KB942_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853106.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="KB942_002737"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="KB942_002737"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBB6853107.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="KB942_002738"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="KB942_002738"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBB6853108.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..>3666
                     /locus_tag="KB942_002739"
     CDS             3200..>3666
                     /locus_tag="KB942_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBB6853109.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
BASE COUNT         1196 a          589 c          800 g         1081 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaa
//
LOCUS       DADVXN010000172         3647 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.178, whole genome shotgun sequence.
ACCESSION   DADVXN010000172 DADVXN010000000
VERSION     DADVXN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.178"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(114..731)
                     /locus_tag="KB942_002740"
     CDS             complement(114..731)
                     /locus_tag="KB942_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853110.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            complement(793..1473)
                     /locus_tag="KB942_002741"
                     /pseudo
     CDS             complement(793..1473)
                     /locus_tag="KB942_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1795..2016)
                     /locus_tag="KB942_002742"
     CDS             complement(1795..2016)
                     /locus_tag="KB942_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853111.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2114..2359)
                     /locus_tag="KB942_002743"
     CDS             complement(2114..2359)
                     /locus_tag="KB942_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853112.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2599..3213
                     /locus_tag="KB942_002744"
                     /pseudo
     CDS             2599..3213
                     /locus_tag="KB942_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1163 a          667 c          538 g         1279 t
ORIGIN      
        1 cttttcactt ttcggaataa attcactgtg actcttgata ccttctttta tatattgcta
       61 atgtattttc tccattgcta tgaaaataga atcaacatat ttataatatc tatctaatct
      121 attttcattt caccgcagca tcttattaaa taacgtgtaa ataagaatat aaaagttatt
      181 gaactaatta ataaagatag aatcatcgta gttggatacg tagatgaaac ctcaactata
      241 gttcttttcc ccgtaccttt aatagacact aaaccattgt cattttctat agtgctcttt
      301 ttcccattta ctttcacaac tgttccccaa taattgaata caggtaatgt gatattttgc
      361 tcatcggcat cttctactgt atagactatt ttggaattat atgctttatg agatgcattc
      421 tcccaccgat caccaacttt tacttcatgt ttctttaaat gatctttata tttcaatgca
      481 tctaatggtg catagtcata taggcctcca gttcttgagg atgtataacg tgtaacatct
      541 tttttggtca taactaaacg acctttatac gactgaattt tatgcgaata atttgcttct
      601 gttgctgcag tcacagtaaa aataataaga actattccta caaggctcca catacttttt
      661 ttgttgcttg ttttccactt taaaaacact atggttaaga ttaatgaacc aaaaagcact
      721 tgaaaaccca aggttctcgg ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga
      781 actccaaata tcttaggctg ttattcccat taataccttg atttcagtag acaccgaaaa
      841 gccgaagagc gttccatttc ttcggttctt tttatatatt cctcgaatgg tctccatgcc
      901 cttaatcgtg gaagaggctg tacggagact ttgataaaat ttatttcgtc gtttaatagg
      961 tcgatggtct tgttctatta aattgttaag atacttcaca gttcggtgct ctgtcttagt
     1021 atataaaccc acactctgta actttctaaa ggcggagcca agagaaggtg ctttatcggt
     1081 cacaattgct ttcggctcac caaactgttt atggagtcgt tttaagaaag cataggctgc
     1141 ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt aagccgtccg catcaattgc
     1201 acgataaaga taatgccaac gtcccttaat tttgatatag gtttcgtcca ttttccatga
     1261 atagaaggat tgtctatttt tcttcttcca aagataatag aggactttgc tgtactctta
     1321 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
     1381 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
     1441 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
     1501 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
     1561 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
     1621 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
     1681 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
     1741 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
     1801 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
     1861 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
     1921 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
     1981 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
     2041 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
     2101 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
     2161 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
     2221 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
     2281 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     2341 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     2401 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     2461 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     2521 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     2581 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     2641 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     2701 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     2761 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     2821 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     2881 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     2941 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     3001 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     3061 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     3121 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     3181 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     3241 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     3301 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     3361 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     3421 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     3481 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     3541 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     3601 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatt
//
LOCUS       DADVXN010000173         3639 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.179, whole genome shotgun sequence.
ACCESSION   DADVXN010000173 DADVXN010000000
VERSION     DADVXN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.179"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            69..152
                     /locus_tag="KB942_002745"
     CDS             69..152
                     /locus_tag="KB942_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6853113.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="KB942_002746"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="KB942_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6853114.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="KB942_002747"
     CDS             complement(1277..2140)
                     /locus_tag="KB942_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6853115.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="KB942_002748"
     CDS             complement(2142..2414)
                     /locus_tag="KB942_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6853116.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="KB942_002749"
     CDS             complement(2431..2640)
                     /locus_tag="KB942_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBB6853117.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2739..3635)
                     /locus_tag="KB942_002750"
     CDS             complement(2739..3635)
                     /locus_tag="KB942_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6853118.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1113 a          643 c          563 g         1320 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattaat cacttgtgat ttgtgattct attctttttc attttctaat
     2761 tgaatgattc gctcaatcat ttcaaaaaat acgttctcat acatagacaa aacttttttg
     2821 tcatagcctt tgtgttctgt aatgccattt ttagaccaag tacttacttt attacttcgc
     2881 ttgataatat ttcggaaaac caaattatcc tctttatgtt ctttgtacag ttcttccagg
     2941 tttgatttta tcgttgcgct gtccgtatca actaaataag gaacaaaacc aatcatatct
     3001 agtccagggt taaactgttc ttgcaaatca atcaaatagg aaatatagtt ttgaatgttg
     3061 tttgtacttt cttcttctgc ttgtaaaggg atcataacgt aatcactcgc cacgattgca
     3121 ttatttgtat aaacgcttgg cgttggtaca gtatcaataa taataagatc atagtcactt
     3181 tttaaaggtg ctaaaagagt agcaagcaat ctactttcat tttcaaacgt ccatgagcga
     3241 gttaattttg gcagtaacat caaatcaaac gtgccaggga tcaagtctaa attatcagtc
     3301 aaatgaataa tagaagaagc caagtttcca tttttcaagc cttcataaaa attgacacgt
     3361 ggcaattcta cctcaaaagt ttttgctaag tcttttgtca atgttgcctg taagtcctta
     3421 tcgatcatta aaacttttaa attcaatttg tctgtcaagt aagcaaacat agtcgataat
     3481 ttggactttc caacaccacc tttaaagtaa ttattcaaga taataattgc ttcattttta
     3541 ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt tttccttctc cataactacg
     3601 ccccattctt tttttgtata gtaatattgt atcattaag
//
LOCUS       DADVXN010000174         3612 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.180, whole genome shotgun sequence.
ACCESSION   DADVXN010000174 DADVXN010000000
VERSION     DADVXN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.180"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..611)
                     /locus_tag="KB942_002751"
     CDS             complement(<1..611)
                     /locus_tag="KB942_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853119.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQ"
     gene            complement(759..1301)
                     /locus_tag="KB942_002752"
     CDS             complement(759..1301)
                     /locus_tag="KB942_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBB6853120.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1436..1825
                     /locus_tag="KB942_002753"
     CDS             1436..1825
                     /locus_tag="KB942_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853121.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1829..2593
                     /locus_tag="KB942_002754"
     CDS             1829..2593
                     /locus_tag="KB942_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6853122.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2722..>3612
                     /locus_tag="KB942_002755"
     CDS             2722..>3612
                     /locus_tag="KB942_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6853123.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGA"
BASE COUNT         1236 a          587 c          730 g         1059 t
ORIGIN      
        1 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
       61 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
      121 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
      181 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
      241 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
      301 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
      361 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
      421 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
      481 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
      541 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
      601 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg
      661 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
      721 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
      781 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
      841 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
      901 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
      961 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
     1021 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
     1081 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
     1141 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
     1201 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
     1261 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
     1321 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
     1381 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
     1441 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
     1501 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
     1561 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
     1621 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
     1681 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
     1741 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
     1801 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
     1861 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
     1921 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
     1981 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
     2041 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
     2101 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
     2161 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
     2221 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
     2281 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
     2341 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
     2401 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
     2461 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
     2521 tatggtacaa atacaactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
     2581 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
     2641 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
     2701 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
     2761 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
     2821 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
     2881 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
     2941 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
     3001 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
     3061 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
     3121 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
     3181 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
     3241 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
     3301 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
     3361 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
     3421 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
     3481 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
     3541 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
     3601 gaaaaagggg cg
//
LOCUS       DADVXN010000175         3515 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.181, whole genome shotgun sequence.
ACCESSION   DADVXN010000175 DADVXN010000000
VERSION     DADVXN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.181"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            81..1820
                     /gene="yfcC"
                     /locus_tag="KB942_002756"
     CDS             81..1820
                     /gene="yfcC"
                     /locus_tag="KB942_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBB6853124.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1977..3161
                     /gene="iadA"
                     /locus_tag="KB942_002757"
     CDS             1977..3161
                     /gene="iadA"
                     /locus_tag="KB942_002757"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBB6853125.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3272..3475
                     /locus_tag="KB942_002758"
     CDS             3272..3475
                     /locus_tag="KB942_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6853126.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1116 a          590 c          817 g          992 t
ORIGIN      
        1 gtcattttta ctgtataatt catttgatta tccatttttt attcatcaaa attactgaaa
       61 aaagaaagga agaaataaat ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact
      121 tcactaaatt atcgacaccc catacgtatg tcattatttt tggtgtagtc atctttgcat
      181 ggatactgac ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt gaatacaaag
      241 atgcaaatgg cgaaacaagt actcgtactg ttttacgtca agattccttc cgttatgctt
      301 acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat catccagcag
      361 aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct gacggagaaa
      421 aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg ctttacgatc
      481 agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc aaaatatggg
      541 gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga ctagtatctg
      601 gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt
      661 ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa
      721 ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca ttcggtggcg
      781 ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt ccatttgtca
      841 ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg
      901 gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg attgctggaa
      961 tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt actgcattag
     1021 ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga
     1081 cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat gaaaataaaa
     1141 aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt gtctggatcg
     1201 tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa ttctttgtga
     1261 tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg aaagtcaatg
     1321 acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca atcgtagtag
     1381 gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt ccttcagctt
     1441 tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct gtcattggtg
     1501 cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca aattcaggac
     1561 aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt gtatccagac
     1621 aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt acacccgtat
     1681 cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa tgggcaagat
     1741 tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc atgattgcca
     1801 ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc caaacagtag
     1861 ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt
     1921 tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa aaatgtatga
     1981 aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg gatgtactga
     2041 ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac gaaggagtgg
     2101 aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt gactgtcatt
     2161 tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat
     2221 tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga acagacggag
     2281 aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca gaaggaatct
     2341 ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca gattctatca
     2401 tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca gtatccgatc
     2461 accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag
     2521 gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa
     2581 agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca caatttttgc
     2641 ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt gccaaacgtg
     2701 gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag acagatggtg
     2761 aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct ttagataatt
     2821 tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac aatcatttcc
     2881 tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa
     2941 aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca gcacgtatat
     3001 tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg
     3061 ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg gtccaagaaa
     3121 aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct ctaactatct
     3181 tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag
     3241 atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac gttctaaaga
     3301 tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa
     3361 gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag aaaaagctcg
     3421 aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc
     3481 acttattact aattgaatca agcaaataaa aaatc
//
LOCUS       DADVXN010000176         3500 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.182, whole genome shotgun sequence.
ACCESSION   DADVXN010000176 DADVXN010000000
VERSION     DADVXN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.182"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..2232)
                     /locus_tag="KB942_002759"
     CDS             complement(<1..2232)
                     /locus_tag="KB942_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853127.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="KB942_002760"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="KB942_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          989 a          774 c          549 g         1188 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta
//
LOCUS       DADVXN010000177         3443 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.183, whole genome shotgun sequence.
ACCESSION   DADVXN010000177 DADVXN010000000
VERSION     DADVXN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.183"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..552
                     /locus_tag="KB942_002761"
     CDS             <1..552
                     /locus_tag="KB942_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6853128.1"
                     /translation="KTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGE
                     GGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAI
                     GVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGI
                     KLRKELTSKNISNIWYEYLNQFF"
     gene            595..1995
                     /gene="wzy"
                     /locus_tag="KB942_002762"
     CDS             595..1995
                     /gene="wzy"
                     /locus_tag="KB942_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HBB6853129.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILLINFFLQQFNKRL"
     gene            2041..>3443
                     /locus_tag="KB942_002763"
     CDS             2041..>3443
                     /locus_tag="KB942_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBB6853130.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCA"
BASE COUNT         1144 a          443 c          627 g         1229 t
ORIGIN      
        1 aaaactacaa gtagtaaaaa atttattact gcatgtagat tggataaaca aaaaaatttg
       61 cccttaatga ttgatgcaat aggaaaactt attcatgaag gatatgagtg tgaactggat
      121 atttatggag aagggggatt aaaaaccgag cttgaaagtt atatcaacca taatggatta
      181 acagaatttg ttcatctaaa agaatttaca aataaaatac atgaaaagat gcaaaatgcg
      241 gcaggatttg taatttcttc tgattatgaa ggtatatcta attctatgtt agaagcactg
      301 gctattggag tgccagttat cgctacagat tgtccagtag gtggcgcaag aatgtattta
      361 ggtgatagaa atgaaatagg tcatcttgtt gaatgtaata atctagataa gttaacagat
      421 gcaatgcgta gggtactttt ggatagagaa agtgtggtaa gaaaagcgca aaaaggaatt
      481 aaactaagaa aagaactgac atctaaaaat atttcaaata tatggtacga atatttaaac
      541 cagttctttt aaccaaaagg aggatattgt gtgaaagaaa aaataaatat attgatgttt
      601 tcgttaacgt tagtcgcgtt tacaatattt tgcgcttttt cattaaatca gaactattgg
      661 ctaaattggc ttggaatatt gatttactta tatgcaatct gtacatggca ttggagaaaa
      721 ggagattcaa tattttcatt atacacgatt tttttcactc tttttttatt atttagttat
      781 gggcaatgca ttatgtgggc attcggtatt ggaacagatc gtggaatcgg tacaacagta
      841 gtagcttatg gtacggggat tattcctagt gaatcagata tgataatggt aaaatggtat
      901 tcttgtattt ctatgtttgt atttcacata ggagcactct tatttacgag aaaaaccaca
      961 ctaagaaaag ttagttggta tgaatctgga gatgctgatg ccgatagaaa gtttttattt
     1021 aaaatagggt gtatcatttc tataatagta gtaccgatag tttttgtttc taaattacta
     1081 gaggtacgta tagcattagt ccatggttat aatgcattat attatggtga ttatgctaca
     1141 caaagtggat atttacaaat tattctatat cttttttttc cagccttaat atgtttatta
     1201 attggtagca acttttcaaa agtaataacg agatttgttt ttattatttt tgctttatat
     1261 tctttattag gaattatgtc aggagacaga ggaagttggt tgtatagctc tattatactc
     1321 atatgggcat acactcaata taaaggaacc tttaattata aaaaattaat aaagtggctt
     1381 attttagctg ttataggaat atatattcta aacgtaataa cgaaagcacg tgatggtgga
     1441 ggactaagta aacttacttt aaaagatttt acatctgtat ttgattcaga tgattctcct
     1501 ttagttgatt ctttttttga aatggggggg actatgagta ttataacttt tttcctaata
     1561 agtggtaatg gaatatatcc ctttgctaat acttatttga cctcactctt aggctctata
     1621 tcaacaagaa tgctatcaat gtttggaata aagtttgttt tattagctga ttggttttct
     1681 caggattatt taggaatcac ttgggggact ggattctcaa tgattgcaga agcctatgtc
     1741 aatggtggat acattggggg tatagtctat atttttatta ttggtatagt catcggtaaa
     1801 atcctttcta atagtataga caaccaagat attgaaaatc atcctatgaa gatgtttttg
     1861 gctataacta cagcaaatat tataatgggt tttcctagag ctgcttcata tttaattgtg
     1921 aaaaattttg tgtatggtgt tttaattata ttgttgataa actttttcct acagcaattt
     1981 aataaacgtc tttaatattt aagcttaata aaaagataaa acaagtcagg agttgcaaaa
     2041 atggaaagaa ctgctcggct caaaaaaaac actatttggg ctctggtgtt tcaaataact
     2101 tctattgtct gtggtatgat cttgctacgt ttgatattaa caaagtacgg atccgaggtt
     2161 aatggaatga ttagctctat taaccaattt ctacaaatta tttctttttt acaattagga
     2221 gtaggcgctg taatgcaatc aactctatac aaaccactag cagaaaagga taatttaatg
     2281 ataagtcaaa tcttagcttc tggagggaag ttttatcggc aaattgcata tattctacta
     2341 gtatacgtgg gtttgttact tgcagtctat tatgttctcg ttcctaatga atttgggaga
     2401 tcatatacaa tggcaatgat tgcaacatta agtattaatt catttgctca atattatttt
     2461 ggtattgtta atactttgtt attaagtgct gaccagaaaa tttatattac gtatattgca
     2521 cagacaatag catttatttc taatacagtt gcatgtgctt tattaattag tctgaacaca
     2581 gagatcctta tggtaaaact tattactgct ataatttatc ttgcttgtcc aatatattat
     2641 agatggtatg tggatcgaaa ctataatata aagaggaatg tgtcatatga taaggaaccg
     2701 atccctcaaa aatggaatgg ggttgcacaa cacgtagctt ctgttgtact agatagtact
     2761 gatgttgtag tgttatctat attttctaac ctgatatatg tatctatata taatgtgtac
     2821 aatttagtaa tatacggagt aaaacagcta ttcatggcta tgacaaatgg cgttcaggcc
     2881 ttactagggg aactttgggt cagaaaagaa atagaagttc tttacaaaac atttgcatgg
     2941 ttcgaatgga ttgtacatac tttgacagtt tttatttttg gctgtaccgg tatactaatc
     3001 gttccattta ttcaagtata tacaagagga gtaacagatg ctaattataa tcaattatca
     3061 ttcgcagctt tacttgtaac tgctaacgca cttcactgtc tgcgtacacc ttatcatgta
     3121 atgataaaag cagctggtaa atataaagaa actcaaaata gttttataat tgcttctttg
     3181 ttaaatttag gtttatcatt aattttggtt aaaccactag ggctagttgg agtagcatta
     3241 ggatctctaa tttcaatcac atatcataca ctttggagtg caaattataa ttcaaaatat
     3301 ttgcttaaat ggccgatgaa aaaattctat aaacagttat ttgtaaacat cttaactgta
     3361 gttctttctt ttggggcgac atcacaattg aaaatgatgt caatcacata tttggattgg
     3421 attgttctcg caattaaatg tgc
//
LOCUS       DADVXN010000178         3415 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.184, whole genome shotgun sequence.
ACCESSION   DADVXN010000178 DADVXN010000000
VERSION     DADVXN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.184"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            393..1832
                     /locus_tag="KB942_002764"
     CDS             393..1832
                     /locus_tag="KB942_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6853131.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2127..2345
                     /locus_tag="KB942_002765"
                     /pseudo
     CDS             2127..2345
                     /locus_tag="KB942_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2594..2809
                     /locus_tag="KB942_002766"
     CDS             2594..2809
                     /locus_tag="KB942_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853132.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3026..3282)
                     /locus_tag="KB942_002767"
                     /pseudo
     CDS             complement(3026..3282)
                     /locus_tag="KB942_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1007 a          591 c          725 g         1092 t
ORIGIN      
        1 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
       61 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
      121 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
      181 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
      241 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
      301 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
      361 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
      421 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
      481 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
      541 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
      601 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
      661 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
      721 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
      781 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
      841 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
      901 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
      961 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     1021 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     1081 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     1141 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     1201 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     1261 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     1321 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     1381 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     1441 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     1501 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     1561 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     1621 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     1681 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     1741 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     1801 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     1861 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     1921 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     1981 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     2041 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     2101 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     2161 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     2221 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     2281 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     2341 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     2401 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     2461 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     2521 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     2581 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa
     2641 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     2701 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     2761 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     2821 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     2881 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     2941 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     3001 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     3061 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     3121 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     3181 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     3241 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     3301 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     3361 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgc
//
LOCUS       DADVXN010000179         3300 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.185, whole genome shotgun sequence.
ACCESSION   DADVXN010000179 DADVXN010000000
VERSION     DADVXN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.185"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(51..2522)
                     /locus_tag="KB942_002768"
     CDS             complement(51..2522)
                     /locus_tag="KB942_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6853133.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2795..>3300
                     /locus_tag="KB942_002769"
     CDS             2795..>3300
                     /locus_tag="KB942_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853134.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT          975 a          664 c          595 g         1066 t
ORIGIN      
        1 aagatacacg agtggttgtt cttttttact cttttttgag atacttttcg tcaattttct
       61 cgtttaaata tcgatagaaa ttttttagct acgcgactaa acggcttttg ctctttttca
      121 tttttacgtt caaacaaata ttctaccaat gtggtcaaat gcctaaaaat cgaatctgcc
      181 gagaaaaata gaatgctcaa cagcaaaagt gatacagctg agataccggt gattgaaaac
      241 agcttatgca aaaagatgac cgaaaaaaag ttaccgctag cacagaaaag aatgatctga
      301 cttctgaatt tattcagcgg tgaacagatt ttatagagaa cgaggaaccc tactgcaatc
      361 aaaagcatgg tagatgctgt agaaacatct gttttattta gattcaaagt caccccgcag
      421 atcaccaacg caccgactac gatcatatct gttagtccac ctggcacagc tttttccatc
      481 acattcatga tgaatttccc tctgattcgc tttttgtttt cttctagagc aagcaagaaa
      541 gatggaagtc cgatagtaaa caaactaatc aatgtgattt gtgatggttc taaaggatag
      601 gtcagagcaa agatcactga aaacagtgag agcaaaaagg agaaaatatt cttcactaaa
      661 aacaagctgg ctgatcgctc aatattgttc acaacgcgcc gtccttctgc aacaacttct
      721 ggcatgcgtg aaaagtcaga atcaagtaag accacttgtg aggcctgcat ggcggcttca
      781 tttccggaag ccatcgctat actgcagtct gcttctttca tggctagaat atcattcacg
      841 ccatcacctg tcatagctac tgtattgtta cgctttatca attgaacgaa gattttcttt
      901 tgttctggct tgacgcgtcc aaaaactgta tatttttcga cagctgcttc atattcttct
      961 tctgataatt tagatgcatc aacgtactga tcagcatgtg aaatccctgc ttggacagct
     1021 acattggata cggtgagcgg attatctcca gaaatcactt tgatggctac ttgctgtttt
     1081 ttgaagtaat caaatgtggc tcttgcttct tttctgatag gattagcaat cagaatatag
     1141 cctaatggtg tgactgcttc tgtcaatact ggttcttcaa gaatcccatc gtacacacca
     1201 aagaccaaca cgcgatagcc tttttcagcg aaagaaacaa actcttccgc atattccggc
     1261 aattgatcgc gcaaaaccat ttcgggcgct cctaaaacat acaccttgtc tgccaacgct
     1321 acactgctga atttttggac agaagaaaaa ggaataacat ggtgatagga ttgatcagtg
     1381 gtttttgtaa aatatgcttt aacagcttcc attgttgcat tgtctcctgc catcgctttt
     1441 gcatagtcag caatgcatgc ttccaattga tctgactgac tgattatttc ttgtttttta
     1501 gagacgatca ttttttgaac agacatttta ttttctgtga tcgttcctgt tttatctaca
     1561 caaaggacat ttacccttgc caatgtctca atgcttttca tattgtgtaa aagaactctt
     1621 tgctttgcta gtttagtcgc acttaatgct aatgcaatcg tcgtcagcaa gtacagtcct
     1681 tcaggtatca taccgatcaa tgctgcttcc attgcaacga cactttcttt gatcgtgttt
     1741 ccattaaaaa agtaactttg agaaaaaagc aggataccga taggaataat gatgatccca
     1801 atccatttga tcaattgatt aagagaagca atcatctccg actgttctcc ttctcccatt
     1861 gtcttggctt tgactgtcag ctgggaaata tacgaatctg ctccaacttt gtctaatcgg
     1921 gcgtaagctt ttccggaaac aatgaaactg ccggacatta atggatcacc tatttctttg
     1981 gttatttcat ctgcttcgcc tgtcaataaa gcctcattaa cttgcagttt cccttccacg
     2041 acctctgcat ctgctggaat ttgatatcca gtctctaaaa taacaatgtc atctattaca
     2101 aggtcttcta tatctattgt ttgttctatc ccgtctcgta tgacattgac ttttgctgta
     2161 tttaaaacat ttagttcgtc caagatcttt ttggatcgaa tttcctgaaa aatggcgatt
     2221 cctgtattgg ctatgataac tggtaaaaaa gtcaagttct tataggattc tactaaacaa
     2281 agcaaaatgg ctaagataag aaagatcaaa ttgaaatacg taaatatgtt ttccaatacg
     2341 atttgctgat ttgttttgaa tgtgggatcg actgaattat ttgctgcacc tttttctatt
     2401 cgttccttga cttgatctgc cgttaatcct tctttatacg atggaaaaag acggatgact
     2461 ttatctgatg atctttgtgt tactttcttt tttttacttc gatttatcca tttattcttc
     2521 atgtggctac ctcgattgat ttcgttttct ggatatttcc aaacttctcc tatccagtat
     2581 agaacagata tggaaagtgt tcctcacatt aagcttgcaa acacctttca ttcctgtcat
     2641 atagcaaaaa aaaagggttc tataatattt tgtgttggtg gaaaatcgaa ggcgattttc
     2701 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     2761 gaccaaaacg tcatcctacc aaaggagtct ctcaatggat aatcatacta gaaaattact
     2821 cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa
     2881 aggcgttcaa gcgcagttta ttaaaggaaa attggatgac tcttcttcct attgtgaaca
     2941 ctgtcgatcg gttcgtatta tccgcaacgg ttcctatacg acacgcactc agatgttgaa
     3001 agtcaaagaa aagttaacta ttttagaatt aaaacgtacc cgttttctct gccatgattg
     3061 tggaaggacc ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga
     3121 actgaagcag gtgattctca tggaactgta cgaaaatcaa tcacgtaaac taatcgctaa
     3181 gaagtacttc gtctcggatg ggacggtgac acggatcttg cgtgaagcaa cgaagcatta
     3241 ccagccacgt atgaacttcc ttccgacggt gttgtgtatg gatgagttca aatcaatgaa
//
LOCUS       DADVXN010000180         3284 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.186, whole genome shotgun sequence.
ACCESSION   DADVXN010000180 DADVXN010000000
VERSION     DADVXN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.186"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(100..1119)
                     /locus_tag="KB942_002770"
     CDS             complement(100..1119)
                     /locus_tag="KB942_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6853135.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="KB942_002771"
     CDS             complement(1116..1340)
                     /locus_tag="KB942_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6853136.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="KB942_002772"
     CDS             complement(1337..1630)
                     /locus_tag="KB942_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853137.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1667..1804)
                     /locus_tag="KB942_002773"
     CDS             complement(1667..1804)
                     /locus_tag="KB942_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6853138.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1806..2237)
                     /locus_tag="KB942_002774"
     CDS             complement(1806..2237)
                     /locus_tag="KB942_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853139.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2251..2745)
                     /locus_tag="KB942_002775"
     CDS             complement(2251..2745)
                     /locus_tag="KB942_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853140.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2745..3194)
                     /locus_tag="KB942_002776"
     CDS             complement(2745..3194)
                     /locus_tag="KB942_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853141.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          884 a          688 c          529 g         1183 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgctc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataatgga acgaaaacca
     1741 ttacttgttc gttagtgaaa cagccccagt catacattag tttgatatct tcatatgaat
     1801 acatgttact tcccaccttg taattgtttt ttgatttctt cgatttcctt ggtattttgt
     1861 acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac tttctgactt ttctttcaat
     1921 aagacatttt cttgcttgat tgctacattg tttagcattg ctttagaatt tagctgtttc
     1981 aattcatcat tttctgcctt cagcgtttcg tataatgcct taatattagc caattcatct
     2041 acgctatcaa cagcattccc ttttttctct tcttcagtag ctaactcaac ccactgtttt
     2101 ttatcaaaat caaatcttgg tctccagtta ggaactggtg gtttaatctc tgtacagttt
     2161 tcagggatat tttcttgatt gttcaaaatg atttgctcga atccgtaagg cttgattgat
     2221 ttgtaaacta ctttcattag atttcctccc ttaaattgaa taagtgataa caaatgaata
     2281 agctgatcca taacttgaat ttcttctcca tttaatggct ccgtctgcgc caatagatag
     2341 ttgagcactg ttcaaagtag aacggtctat tgacccaacc agttgttcaa aactaattgg
     2401 tgtccgatag ccttctggaa cagttaaaat aaccgaatca ttcccgccac tactttttcc
     2461 gtttaaagcc acaaaatata tagaaactgt ttttccttca cgataaagct ttgctgtccc
     2521 tgtattaccg ttagtgacaa ccagatttac cacttcatat ctgttatcat cagtcgtcaa
     2581 aacacgttta ttattgattg ttacagaacc tgaaaaattc ttatgtcctg caatggcttg
     2641 gtctccatct gtctgcacca atgcttcttt accatcaata gcttctacat gcgtttttaa
     2701 gtatttagca actccgtctt cttttaattg aacaatatca gccattaaac cgtccccact
     2761 ttctcaaatg taatattcgc taatccatca agtttcactt tatcggttgc tgacattaaa
     2821 cctgctgtcg tagttgtagc attacctgga tttttctgtg ctccagctgc aattccatcc
     2881 aacttagttt tatctgtgga cgacatcaac ccatttgctg tagttgtagc tacagctgta
     2941 gttgtggcat ttattccagg atcaccttta tctccctttg gtaaaacaaa attaaatcta
     3001 gctgcagatg atgttcctac attcgtaaca gaagcggttg aaccactaga aacggttcct
     3061 atggtaattg ttgctgcttg gccaggatcg cctttatctc ccttcaccgt tgttggtttg
     3121 ccttctatag cattccaatg agtttgtgga taaacctgta cattgctttg ttttattttt
     3181 acgatatctg tcattctcta tacctcccca attttttcaa aagtaaaatt tggaattctt
     3241 tcgtttgtgt aattttctgc ttgatttaca gcttcttgga attt
//
LOCUS       DADVXN010000181         3283 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.187, whole genome shotgun sequence.
ACCESSION   DADVXN010000181 DADVXN010000000
VERSION     DADVXN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.187"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            63..878
                     /locus_tag="KB942_002777"
     CDS             63..878
                     /locus_tag="KB942_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853142.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1004..1261
                     /locus_tag="KB942_002778"
     CDS             1004..1261
                     /locus_tag="KB942_002778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853143.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1513..2085)
                     /locus_tag="KB942_002779"
     CDS             complement(1513..2085)
                     /locus_tag="KB942_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6853144.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2101..2706)
                     /locus_tag="KB942_002780"
     CDS             complement(2101..2706)
                     /locus_tag="KB942_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBB6853145.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3107..>3283)
                     /locus_tag="KB942_002781"
     CDS             complement(3107..>3283)
                     /locus_tag="KB942_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6853146.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
BASE COUNT         1078 a          611 c          501 g         1093 t
ORIGIN      
        1 tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa ggagcaataa
       61 gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa ctttataaac
      121 aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac tttattctac
      181 caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat ctgtattact
      241 gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc atagcagagt
      301 ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt gatttaggta
      361 tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt cctctctcta
      421 gttactataa attagccctt gatagttctt taaaggatgt taccgagagc actgtccatg
      481 aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa aatgaggctg
      541 atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga aaaacacaaa
      601 aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat ttcgaattaa
      661 gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa gatctagaag
      721 ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt gtaaacacta
      781 gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata gagcttacta
      841 aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc catcattaag
      901 tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg cttactattt
      961 attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta atttatatat
     1021 agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg taaaatcaat
     1081 aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga tttttatgat
     1141 agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg atatggagat
     1201 aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc cacgttccta
     1261 aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg ttcattataa
     1321 atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt actttacctt
     1381 ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa tattatattt
     1441 cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt ctggttcgct
     1501 gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg tactgcgact
     1561 gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca gttcatatgc
     1621 gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat taggattatt
     1681 ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac gttccatttc
     1741 tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt caatcgttcc
     1801 tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga tctctaatgc
     1861 ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat ctccaggctt
     1921 cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg taaatttttc
     1981 tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt gttcttttgt
     2041 actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt ccacctactt
     2101 ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga catcgattcc
     2161 tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa ttagatagcg
     2221 tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg ctttatcgat
     2281 taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca gccaaattct
     2341 agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca catatttctt
     2401 gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg aatgttctaa
     2461 atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc
     2521 ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac ctttaaatgt
     2581 accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt tttgtttact
     2641 gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt tattctctaa
     2701 aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct caaccccatt
     2761 atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg atagattttt
     2821 caagtgtctc tattttgcac agtctagttg acctttgata cacctctata tttatagaga
     2881 atctaaaaca tcaacatggc atactcaaaa ttctaaatat tagggataaa gaattctcct
     2941 atacaatcaa aaatttcctt tatttcgaac tccggtcacc taactctatt gatctcttac
     3001 aagccaaaga atatctctat actacaaaga aacaataggg tgcctggttg gtctctttgt
     3061 gttttatttt agaatctaga acgtaaatat tcttgtcatt ctgattttat ctaagaatat
     3121 caattaaatc tttttttgca gcctctgaag cgggcattac agaaaagacc agtccaataa
     3181 ccgaagatac cccaaccgct agtaaaatag taaatagatc aagtgacacg tttaccttta
     3241 tcaaagaacc aacactataa gcaagagcca ttcctaataa ata
//
LOCUS       DADVXN010000182         3232 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.188, whole genome shotgun sequence.
ACCESSION   DADVXN010000182 DADVXN010000000
VERSION     DADVXN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.188"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..149)
                     /locus_tag="KB942_002782"
     CDS             complement(<1..149)
                     /locus_tag="KB942_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBB6853147.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYS"
     gene            complement(139..1338)
                     /locus_tag="KB942_002783"
     CDS             complement(139..1338)
                     /locus_tag="KB942_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6853148.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="KB942_002784"
     CDS             complement(1468..2844)
                     /locus_tag="KB942_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6853149.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3232)
                     /locus_tag="KB942_002785"
     CDS             complement(2844..>3232)
                     /locus_tag="KB942_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6853150.1"
                     /translation="LIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQ
                     QQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEV
                     ADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          992 a          614 c          457 g         1169 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa at
//
LOCUS       DADVXN010000183         3214 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.189, whole genome shotgun sequence.
ACCESSION   DADVXN010000183 DADVXN010000000
VERSION     DADVXN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.189"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            152..841
                     /locus_tag="KB942_002786"
     CDS             152..841
                     /locus_tag="KB942_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6853151.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            855..2357
                     /locus_tag="KB942_002787"
     CDS             855..2357
                     /locus_tag="KB942_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6853152.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2370..2846
                     /locus_tag="KB942_002788"
     CDS             2370..2846
                     /locus_tag="KB942_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6853153.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2943..3053)
                     /locus_tag="KB942_002789"
                     /pseudo
     CDS             complement(2943..3053)
                     /locus_tag="KB942_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1061 a          435 c          618 g         1100 t
ORIGIN      
        1 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
       61 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
      121 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
      181 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
      241 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
      301 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
      361 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
      421 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
      481 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
      541 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
      601 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
      661 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
      721 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
      781 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
      841 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
      901 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
      961 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     1021 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     1081 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     1141 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     1201 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     1261 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     1321 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     1381 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     1441 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     1501 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     1561 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     1621 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     1681 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     1741 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     1801 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     1861 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     1921 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     1981 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     2041 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     2101 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     2161 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     2221 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     2281 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     2341 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     2401 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     2461 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     2521 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     2581 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     2641 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     2701 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     2761 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     2821 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     2881 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     2941 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     3001 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     3061 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     3121 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     3181 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DADVXN010000184         3208 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.190, whole genome shotgun sequence.
ACCESSION   DADVXN010000184 DADVXN010000000
VERSION     DADVXN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.190"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            70..531
                     /locus_tag="KB942_002790"
     CDS             70..531
                     /locus_tag="KB942_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBB6853154.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            565..1200
                     /locus_tag="KB942_002791"
     CDS             565..1200
                     /locus_tag="KB942_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBB6853155.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1313..1948
                     /locus_tag="KB942_002792"
     CDS             1313..1948
                     /locus_tag="KB942_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853156.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            2247..>3208
                     /locus_tag="KB942_002793"
     CDS             2247..>3208
                     /locus_tag="KB942_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6853157.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         1299 a          462 c          537 g          910 t
ORIGIN      
        1 aacacgggct ctttcctcat atatgagctt ctacaaaaaa aatcatatca cagaaatgag
       61 gaagaaaaaa tggaaaaaga gattttaata aaatctccag gaaaaacctt gattaaagta
      121 actgatgatt ctatttcaat tattagaaaa ggatttataa atttagttaa tcaaggtatt
      181 aaaggagaaa aaactatccc ctttaaaaat atatcagccg tacaattaaa aaaaccaggt
      241 atgagtaatg gttatataca atttaccttg ctaggcggaa acgaaagtcg cggaggtata
      301 ttagcggcca cgaaagatga aaacaccgtt atgtttacaa aaaaatattg gaatgaaatg
      361 gaaaatttaa aaaaatatat agagaagcaa caatctatat tagataataa ttccaaagaa
      421 acacaaattt caagtgcaga tgaaattaaa aagtataaag agttgttaga tgaagggatt
      481 attaatcaag aagaattcga ttttaagaaa aaagaattgc ttggtttata atattcaata
      541 tcaaaagaga aagaggatct attaatggac ggtatatttg gttttactgg tattgtttta
      601 tttattatag gtttaatcat gttaattgta agatttatca aaaaaacaaa taagaaaaca
      661 cctatgattc tccttattat cgggattatt tttactaccg taggtttctc gctttcctca
      721 tcaaacgagg caaatgacaa taacaaacag gaaagctcat catctgatac tcaaactact
      781 acatctaaca gcagcaataa aagcctaact caatttgaag aatttttaga gacaaataat
      841 catgactggg gtgctttctt agatagttat tattctatta ctccagctac tgagcaaaat
      901 acagctttta ctaaatatat tagtggtaaa acatatactt ttgaaggaac tgttatcgaa
      961 tcaatgacta ctagaatagc cattatagca gacaaagagt acgatgataa aagttggaat
     1021 gatatttctg caacacctaa agtttcatat gtaatttttg cgaaggactt aaacaatgct
     1081 gataccttta caaagggcga taaagtaact tttacaggag aaattagttc aagaggatcg
     1141 aacatagaaa aatcctatgc tcaatgggat atgattaata gtaaagtaag taaaaaataa
     1201 atgaaccacg ccccactaag agttagattg tgcacaataa taaaacctat acaaaagagt
     1261 gccatcagga gtcacaatgc ttgtaaagga aatgacaaaa taggaggtaa ctatggataa
     1321 tagcaatgaa aaactaacca aaaaagaata tcttgaatta atagttgaag gcggtattgg
     1381 agcaattcct tatattggtg gcgttgtgca gtcactttat tttgggtcta aaaacgagaa
     1441 gcgtttcaaa cgcatcgaga aattctacaa aaatttaaac gaatcattag aaaacattcg
     1501 cgatcaaatt ccagaagatg ttttcaactt agagaacaag gaccaactca ttggaatatt
     1561 cgaagggata aatgacgagg tagaaaaatc aaaggcacaa aataaaatag atatgtataa
     1621 aaatctttat aagaattgtt tacttcgaat taacaacgct tcatgggata acgaggagta
     1681 ttatcttctt gtactgaatc aattaacatc gattgagatt caattgttag catgcttaat
     1741 gagtcgtgga aacgaaaatt tcactggaaa tatcagcatg tctggctatt cacaagagct
     1801 aatagatggt agcttaaata gattatctga ttttggtctg ttagaaaaaa cgattagctc
     1861 catagtatta ggtgatgtcg gcaaacaaaa tatgggctac aaaatatctc atttaggaat
     1921 tcaatttatt aactcaactc ttacttaagt tttctttcta atatgacatc tagacctatc
     1981 agcaatgcaa caataaaaaa ccagacatac caataatcaa caaataatct aaactctgga
     2041 agttcagtta ttcttttaaa aatcacttta atcataataa acacctctga ttggatgatc
     2101 taatacacat ccaattatac cattaaaacg acaaatagca catccttgcg ccggcaagca
     2161 aaaaggatgt gcaaaaaata ataaaaccca aacaatgggc ttctttatag ttcctattgt
     2221 atcagagaaa gagagctgat tcaattatgg caagattagt caaacgtgga aatagttggc
     2281 aatacgaaat ttcatacaaa aaagatgacg gaaaatacac gaagataaga aaatcaggat
     2341 ttaagacaaa aggagaagca aaagatgccg ccaacgaatt agaatataac ttgaacaaag
     2401 gccttaaagg ggatcgcaaa aatctattat tatcagatta ctttgaggat tggatgcaac
     2461 tttataaaga aggaacagta tctcctatca cttatagaaa atacgaagat acgttaatga
     2521 acataaagaa atatatgcca gcggtattga tttctgattt agatagagtg ggatatcaac
     2581 gctttttaaa taagtatgct aaagaccatg taaaatctac cgttattaag tttaataacc
     2641 atattagagc gtcgttgaaa gatgccgtag aagaaggatt aattccgttt gatccaacta
     2701 gaaaagcagt aatcaaagga aaagattcat tgaagccaaa agaagataaa tatttagatt
     2761 atgatcaatt taaatcttta atgaaactcg tagaagaaaa cctttctgcg cagtactctt
     2821 ctcctatgct cgtgttagtt gctggtgcca ctggaatgcg atttgctgaa cttctaggat
     2881 taacatggga agatatcgat ttcgaagatc aaatcatcac tattaataaa acatggaatt
     2941 ataaattaaa tgaatgggga aaaacaaaaa acgaaacttc aaataggaaa atttccattg
     3001 ataaacatac gattgatctc ttaaaaaagt ttaaaatcaa tcaaaaagaa ttattcgaga
     3061 attttgaaat caaaaaccct cataattttg tttttttcaa cttaaaaaat ggattagttt
     3121 catcaaacgc cgtcagcaaa tatttgcgca aaaaattaaa agaattaggg attgaaaagc
     3181 aatttacttt gcatggacta aggcatac
//
LOCUS       DADVXN010000185         3024 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.191, whole genome shotgun sequence.
ACCESSION   DADVXN010000185 DADVXN010000000
VERSION     DADVXN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.191"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            337..1662
                     /locus_tag="KB942_002794"
     CDS             337..1662
                     /locus_tag="KB942_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6853158.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1655..2005
                     /locus_tag="KB942_002795"
     CDS             1655..2005
                     /locus_tag="KB942_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853159.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1987..2172
                     /locus_tag="KB942_002796"
     CDS             1987..2172
                     /locus_tag="KB942_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853160.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         1079 a          471 c          560 g          914 t
ORIGIN      
        1 ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg aatactccta
       61 ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt ctaatataac
      121 taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta atagtaaata
      181 tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga agtagggaaa
      241 taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca aaaacgaacg
      301 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      361 gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      421 aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga taatacagga
      481 tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat ttctaatgtc
      541 agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt tccaccacgc
      601 atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag gtatgtagca
      661 gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac cagatcaatg
      721 aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc tcaaatgatc
      781 caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg agataatccc
      841 ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt ccttgctgaa
      901 tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac agacctttgg
      961 ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag tattaaagac
     1021 ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg cttacagctg
     1081 tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga accgacaaag
     1141 gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag aaatgaaatc
     1201 gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca acataactgt
     1261 aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag accaggtttt
     1321 tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa ttactgtctt
     1381 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1441 aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1501 gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1561 attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag tacattggtt
     1621 ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg atcgtggacg
     1681 tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg ccgacaatga
     1741 acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg ttgcggaagt
     1801 attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag aacgaaacgc
     1861 tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg agctggggat
     1921 ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat tttaccagga
     1981 taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     2041 tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa tgaggttggt
     2101 gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata cgaatactat
     2161 attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt tgtatcaatt
     2221 ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca accttaggac
     2281 gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa gcaatgttaa
     2341 acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa tatagttgaa
     2401 taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa gtaaaagaaa
     2461 tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag ttagaacctt
     2521 tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa gagcgagctg
     2581 catccatgcg gacataggtt cttttgacac tattaattct cctttttagg ataagggaaa
     2641 ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct agtgctgctg
     2701 tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa acaaacacat
     2761 ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact tactcctgct
     2821 gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca ccattacctg
     2881 tagataaaaa ccaatatatt tactatactc actatattaa atatatttag tatagtaaaa
     2941 actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca tattttgaag
     3001 gattaggatt aataaagcta aaaa
//
LOCUS       DADVXN010000186         2904 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.192, whole genome shotgun sequence.
ACCESSION   DADVXN010000186 DADVXN010000000
VERSION     DADVXN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.192"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..1087
                     /locus_tag="KB942_002797"
     CDS             <1..1087
                     /locus_tag="KB942_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6853161.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="KB942_002798"
     CDS             complement(1305..2447)
                     /locus_tag="KB942_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6853162.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="KB942_002799"
     CDS             complement(2452..2637)
                     /locus_tag="KB942_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6853163.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          965 a          531 c          453 g          955 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caat
//
LOCUS       DADVXN010000187         2786 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.193, whole genome shotgun sequence.
ACCESSION   DADVXN010000187 DADVXN010000000
VERSION     DADVXN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.193"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            206..1213
                     /locus_tag="KB942_002800"
     CDS             206..1213
                     /locus_tag="KB942_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6853164.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1225..2229
                     /gene="neuB"
                     /locus_tag="KB942_002801"
     CDS             1225..2229
                     /gene="neuB"
                     /locus_tag="KB942_002801"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBB6853165.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2226..>2786
                     /gene="neuC"
                     /locus_tag="KB942_002802"
     CDS             2226..>2786
                     /gene="neuC"
                     /locus_tag="KB942_002802"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBB6853166.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSF"
BASE COUNT         1020 a          352 c          537 g          877 t
ORIGIN      
        1 tacgaataaa caattatttg acacaaaaaa aatatgtatc ataataattt ttgtatcaat
       61 tttttctttg atacttcata ttttaatcaa tcaaatgttg attcgttacg ttttgacatc
      121 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
      181 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      241 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      301 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      361 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      421 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      481 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      541 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      601 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      661 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      721 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      781 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      841 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      901 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      961 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
     1021 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
     1081 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1141 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1201 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1261 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1321 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1381 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1441 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1501 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1561 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1621 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1681 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1741 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1801 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1861 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1921 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1981 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     2041 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     2101 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2161 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2221 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2281 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2341 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2401 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2461 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2521 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2581 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2641 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2701 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2761 ttttaaataa tagattacct agtttt
//
LOCUS       DADVXN010000188         2773 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.194, whole genome shotgun sequence.
ACCESSION   DADVXN010000188 DADVXN010000000
VERSION     DADVXN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.194"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            21..227
                     /locus_tag="KB942_002803"
     CDS             21..227
                     /locus_tag="KB942_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853167.1"
                     /translation="MSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISI
                     IGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(318..683)
                     /locus_tag="KB942_002804"
     CDS             complement(318..683)
                     /locus_tag="KB942_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853168.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            891..1373
                     /locus_tag="KB942_002805"
     CDS             891..1373
                     /locus_tag="KB942_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853169.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1570..2460
                     /locus_tag="KB942_002806"
     CDS             1570..2460
                     /locus_tag="KB942_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBB6853170.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          944 a          446 c          541 g          842 t
ORIGIN      
        1 gttttagaac taaacgaagt ttgtcttcta aaaaaaattg ggtatactca caaactattt
       61 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
      121 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
      181 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
      241 agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
      301 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
      361 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
      421 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
      481 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
      541 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
      601 cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg
      661 tttttccagc aagtttttat catctttcat caccacctaa caagtgttat tattttgtta
      721 gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa
      781 tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga
      841 tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa
      901 aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt
      961 ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg
     1021 gatttgtggt ttctaggaat tttccagctt tgattgtttt ctatttagtg ataagtgtag
     1081 gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt
     1141 gcttattagt atttacatct acgttttctt tgctgcctcg cttgattatc ggccctaatg
     1201 gtttgagcga gagtggattg aaggatttag cttattcctc catgtatttc ttgacgatca
     1261 aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga
     1321 cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga
     1381 tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt ttctcatatg ctttttttat
     1441 tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa ctatattttt
     1501 acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa ataagaaagg
     1561 agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct tttagccgga
     1621 tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc aagtgactca
     1681 aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt agtcgatgca
     1741 gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca aggaggagga
     1801 agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa ctctgatgta
     1861 tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa agtagcagtg
     1921 gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac caaacaggaa
     1981 ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg gaaagatcaa
     2041 aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg
     2101 ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg aacagttcgg
     2161 cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata tctcgatgat
     2221 tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt agcagataac
     2281 agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc
     2341 caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt caaagaattg
     2401 ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa tatcaaataa
     2461 ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac tatgtttccc
     2521 cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga
     2581 gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag ctacgacata
     2641 gaactgtcgt aacttttttt ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat
     2701 aaaacttaga ccgatatgcg gcgcgttctg tgcgccgcat atttttttag ttttcaaaga
     2761 tcagaccttg ttg
//
LOCUS       DADVXN010000189         2770 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.195, whole genome shotgun sequence.
ACCESSION   DADVXN010000189 DADVXN010000000
VERSION     DADVXN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.195"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(77..1876)
                     /locus_tag="KB942_002807"
     CDS             complement(77..1876)
                     /locus_tag="KB942_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6853171.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2000..2596)
                     /locus_tag="KB942_002808"
     CDS             complement(2000..2596)
                     /locus_tag="KB942_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6853172.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          978 a          522 c          480 g          790 t
ORIGIN      
        1 aaaaaacgca tatacctgcg gtggcaggtt atgcgttttt tgttgtataa agactttttt
       61 cattaacgat ttttgattag ccgactccca aaacaaaatt tagcagttta tctgaataag
      121 tgatttcttc atctgtttgt ttcacacttt catttagtag tcccatctca tcggttatga
      181 taccatccac accataaaac atcatgcgac tcatagcatc tgaatcgttg attgtccata
      241 catatacttc ttttccgtct tggtgggcag aattaatgaa gttacggttg atcgtactat
      301 attccattgt cagaaaatca gcaggcgtca cagggggccc aacaatattg aaaggtaaga
      361 tgtagcccac ataaaagttt ggttctgctt cttttaaatc tgaaacggtt tggtacgtca
      421 aactttgcaa aatatgatgg ttctctaaga taactttgcg gtatttatgg acaaatcgtt
      481 cgacaaggtc atccgaatct tttttagagg tcttgatctc aataagtaat ttttggttta
      541 gcttatgtgc tttttccaaa taagaatcaa aagaacaaac aggggcttgc actccgtttt
      601 cagtaactgt aagatttgtc aattctttca atgtaagatc ctgtggtgtt ccatttaccc
      661 ctgttaagtg acgaagattt gcatcatgca tcactacgaa atgaccatct tttgtttcct
      721 gaacatccat ctctacataa tcaggatgat agtctctgct tgttttttca agtgcttcta
      781 aagtattttg cgccccattt tgggtactca cacctctgtg agagacagtc actggttttg
      841 tctgaacagc tgaagtcaga taatccatat tgtaaagaca gacgccaata ccgaaaaaaa
      901 ctgcaaaaag agtcaaacca gtgtttttca gcgcagagaa acgcaagttg ggggcttgtg
      961 ggataaacca cttagggatc tcaggcaaga agccttcgtc atccatgaaa tcaacaatga
     1021 tataaaaaat ccctacggta gacatcacga tattgaataa taaaatccct tgtaaaagcg
     1081 tcatagcaaa cacagcgctg atcaatgcat agtctgggaa aaattgttca accatacttt
     1141 gtgccaaaat aacaacgata tatcctaaag aagaaaggag taaaatcgta ccgccaatca
     1201 caataaattg gcccgtgatg gccaaaagac gcgatttggt caatgaccag ctttcccgaa
     1261 tggctgctcg aaaaggacga tctcgtagaa tcatttccgg taaagcaaaa atcagtcgaa
     1321 tcccaatata gccaaggaaa atataaacga gtaaaaagga actgacgatg atccaacgat
     1381 tagcaaaaat aaaatccata atgaaagcgg ggatttttat ttttgacagt agatcagaat
     1441 taaaacttaa tccgctgatc ggtagaatca ataaaaaata agcaaggaaa aacaaaaaag
     1501 taatgggtct cactttcttc agctgaagta tcgtcaagtg taagagttgc ttcaaagaga
     1561 ttggttcttt ctttttaata aaaaaaacac tcatcaaaag aaaagtaaat tcgaaataaa
     1621 ctaacagtaa aatcgtcatc aaaactacga ttaacgagag tagaacccca ggatgttgag
     1681 aaaaaattga aggaatattg tcataagaca aatagtcaat tgcccctcgg tgtaaaataa
     1741 aacgggtcgt actgctcaat aagggtaaac aaataaataa aataaagcca tgcatcagca
     1801 agacatcacg gaaataagct tgtgttcctt ttaaaaagtc ccaagcatta cctaagctac
     1861 gtttcaaata attcatccta tcacctgctc ttcacattgt agcgcaaatc taatgaaaaa
     1921 ggaaagggtg cagtgtgttt tgcagaccct ttcccagatt taacattgtc aatcaatgac
     1981 taattttaca aaataaatat tatccaagaa gaccacttag atattgacct ccataaatca
     2041 aggccatact atataagaaa atcgacagtg gtttagcaat caaaatgatt agtgtatatc
     2101 gtttcactga catgtttgtc aatccagcca tcaaacataa cgcatcgtca ggagcaactg
     2161 gaagaaaaat agctagagca aacagcttct caaaacggtt ttgattatcc agccagccaa
     2221 tatatttgtt atatgtctta tcgctaatca tggaaagtat gaatggcttt ccataacgac
     2281 gccctagcag aaaaatgata atcgaaccaa tgcagattcc aatatagtta tagataaatc
     2341 cagcataagg gccaaatatc aggacaccag ctgcagtact gatccctcca ggaatgattg
     2401 gaataactac ttgcaggatt tggatgaaga caaaaataat cggtcctaaa atgatggaat
     2461 cacctactaa tcctcttaac gcattcaaat ctttgaaaac acctaaatta ataaaataaa
     2521 tcgtcagcgc gattgatagg cctaatccga taatggatat gaaatttatc agttttcgag
     2581 aggcagcaat attcattatt catcaactcc ttagaggttc tttgttaact atactttact
     2641 agaaaagcaa ctaactgcgc cattttttga tatgacagaa aacaaacaat tttgtaagtt
     2701 catcataagt ataaaagatt gatctcaaaa aaacatccaa ctaaagactt atttaataac
     2761 ttgttttttt
//
LOCUS       DADVXN010000190         2646 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.196, whole genome shotgun sequence.
ACCESSION   DADVXN010000190 DADVXN010000000
VERSION     DADVXN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.196"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(901..1419)
                     /locus_tag="KB942_002809"
     CDS             complement(901..1419)
                     /locus_tag="KB942_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBB6853173.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2537..>2646
                     /locus_tag="KB942_002810"
     CDS             2537..>2646
                     /locus_tag="KB942_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853174.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          878 a          478 c          389 g          901 t
ORIGIN      
        1 taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact tataaattga
       61 tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat
      121 tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg
      181 aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa taaaaactaa
      241 tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt
      301 tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta
      361 ataggatagg cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt
      421 atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt
      481 tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg
      541 acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac
      601 tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa
      661 gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat
      721 ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga
      781 gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg
      841 ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat
      901 ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac
      961 tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc
     1021 ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt
     1081 aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata
     1141 ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg
     1201 cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca
     1261 accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc
     1321 cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat
     1381 ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc cataaattcc
     1441 taattgtctc ctttaattca tctataattt catccacgat attttaatcc gttctacaat
     1501 gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa
     1561 cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct
     1621 tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt
     1681 aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag
     1741 ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa
     1801 atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat
     1861 tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa
     1921 aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc
     1981 ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt
     2041 gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt
     2101 cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt
     2161 actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca
     2221 agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata
     2281 gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc
     2341 tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta
     2401 ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc
     2461 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
     2521 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
     2581 acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga
     2641 actaac
//
LOCUS       DADVXN010000191         2629 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.197, whole genome shotgun sequence.
ACCESSION   DADVXN010000191 DADVXN010000000
VERSION     DADVXN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.197"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..285
                     /locus_tag="KB942_002811"
     CDS             <1..285
                     /locus_tag="KB942_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB6853175.1"
                     /translation="LSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNI
                     YIENELTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            412..1047
                     /locus_tag="KB942_002812"
     CDS             412..1047
                     /locus_tag="KB942_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBB6853176.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            1166..2377
                     /locus_tag="KB942_002813"
     CDS             1166..2377
                     /locus_tag="KB942_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6853177.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
BASE COUNT         1016 a          410 c          466 g          737 t
ORIGIN      
        1 ttatctcatg aacttgcaca tttcagcgcc tctcattttg aattttcagt tttgtacgat
       61 acttctacaa cttttcactc aaaatttgaa acagaagcag ataaaatagc tattctaatt
      121 ttattaaata tctatattga gaacgaactg actgatgaat ctcagtttaa cctcgaaaaa
      181 tttatggaat attattctat tcagaataag ctcagataca cttgttatgc tgtctgccaa
      241 tgctatttta agaaaaaata ttcttacgca aggcaatatg tatgaataca tcaagaatta
      301 ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt gctggcacac ggactcatac
      361 ctcatttctg agatcgcaaa tatattataa caagaagtga ggaatattta aatggcaaaa
      421 aaagttatgg gtcaagatgg gaaaatgtat aaggttaaaa aaccgtttta taaacgggta
      481 tggttttggg tattaatagt acttttgttt tttggtattg gtggtgctct aagtggcggt
      541 gaaaacaaag attcagctac tgcaacatca acaagtacta ataaagaaac tacaaaagaa
      601 actacaacaa aagaagaaaa gacttataaa atcggtgatg acgttgctgt agggaaaatg
      661 gaatacaaag taaactccgt tgaagtagta aaacaagttg gcccatctgt acttcctact
      721 aacgcaaaag acactttctt agtagttgac ttatctgtaa aaaatgcagg tgataaggct
      781 gtgactgttg atagctcatt cttcaaatta aaagcagatg gaaaaacttt cgaagctgat
      841 tcggctgcat ctatgtcggc aaatcaagat gaaaatggta atatcactaa ctctttcttt
      901 atggaaagct taaatcctga tatgcaacaa actggtaaaa tagtttttga tatctctgaa
      961 gcccaagcaa atgcaaaaaa taatgtattg caagcacaaa caggttactt tggaacagag
     1021 acagtttcaa ttgctcttca caactaatta gtataaaaaa cacgccccac cgaccaaagc
     1081 gagcgtgttc taagaaagaa acaaacctat acgataggct tctttatagt acctattgta
     1141 tcagagaaag agagctgatt caattatgtc agaagtaaca ggatacttag aacaagtaga
     1201 caacgaaaca tatagactaa gagctgtatt aggatacaaa cctgatggct cggcaaaaag
     1261 aaaaagcaaa actataaaag caagtagtaa aagagcggca tataaagaat tgaacatctg
     1321 gttagagcaa ttcgaaggaa tgaccgatga ttcgttggat ttgttcaata taacttttgg
     1381 tgaattttat agaaaaatat ggctttcaga ggccgaaaaa aatttagagc caaagtctta
     1441 tcataactat aagcgaatga tcgagaatag atttcttgat aaatttgatt ttatccctct
     1501 aatcgatatc aaaccataca tgataaagaa aattgttgtt aatgctcaaa gaatcaatac
     1561 aaaagatcct gggagaaact ctgataaacc tttatcaaga aatacaaagc ttcgtatgtt
     1621 atacgccgtt aacaacttat ttttgatggc taaaaatgag tacggagcaa ttaaagaaaa
     1681 tcctgtggaa aatgtaaaaa taccaaaaga aaaaggcgta aaaaagaata tcgaagaacc
     1741 ttattcggaa gaagaaattc atgcaatgct aaaagcagca tttgaggaaa gcatagaaat
     1801 taaaacttta attgttctag catttattac aggagcaaga caaggagaaa ttgcagcttt
     1861 agaagaaaaa gatattgact ttgacaaaca agaaataaga tttcatcagc ggatttcaga
     1921 agtggatgga aaatcagata ttcggttgct gccaggtttg aaaaatgatg atgatgaaaa
     1981 aatagtaacc ggtcctgctt atctatttga tatgttagat gaattaatca aagagaataa
     2041 aaagattcgt tggaaattaa atataaagaa attaaaacac tattttatat ttgatacaaa
     2101 acaagatgga actttgccac gtggaagtta tctgtataag aagttcaaac gattcaccaa
     2161 acgtcacaat ttgcgtcaca ttcgttttca cgacattaga catacatcag ctacctattt
     2221 gcttagcgat cctaatatga ctccaaaaga gcttcaaaaa cgattgggac accgagattt
     2281 taatactaca atgaatgttt atggccatgt cttacgcaaa gaaaaagata cagcaactac
     2341 agcgtttgaa aacctattaa aaaaagatat aaaataacac taaaaaatta tattttcggg
     2401 tataaactcg ggtaggatat acaaaaactt gggataattt gcggtatata agcatttaaa
     2461 aatgctgtaa attcgcttat cttcatttaa aataccatta aataacgaac tcttaatcag
     2521 cgggtcgcgg gttcgagccc ctcacggccc attgggtgcc aaacccacgg aacaggtaaa
     2581 tgttggtgcg aaagcatttc gaaaacattt acgctgttct ttttttatt
//
LOCUS       DADVXN010000192         2623 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.198, whole genome shotgun sequence.
ACCESSION   DADVXN010000192 DADVXN010000000
VERSION     DADVXN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.198"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            21..1472
                     /locus_tag="KB942_002814"
     CDS             21..1472
                     /locus_tag="KB942_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBB6853178.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1821..2453
                     /locus_tag="KB942_002815"
     CDS             1821..2453
                     /locus_tag="KB942_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBB6853179.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          984 a          377 c          515 g          747 t
ORIGIN      
        1 aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa
       61 tgtcccaact ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca
      121 aaattgcaaa agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga
      181 gtagtgaact tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt
      241 caacaacttc atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat
      301 caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta
      361 gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt
      421 cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag
      481 gacaaacaaa aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac
      541 aaccaatact atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt
      601 ttattatgaa aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt
      661 ctgttatgat tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg
      721 caccgaatta caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg
      781 taacaaacga agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt
      841 atgaaaacta tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg
      901 gaaataaaga tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa
      961 ctaaattcct tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat
     1021 taattaaaac ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta
     1081 agactggata ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg
     1141 gaaatgaatt tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag
     1201 ttaaagctgg taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga
     1261 tacaacacga agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa
     1321 ctggagataa tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta
     1381 ctggcccaca ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag
     1441 ttgatccgaa aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca
     1501 agaaaatggt tggattctta gacctttgat caggatattg agactggaat tgatatctcg
     1561 attacgatgg gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc
     1621 gtctttatgc tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag
     1681 gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga
     1741 aaaattggaa atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct
     1801 ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga
     1861 ttactcataa tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg
     1921 tatatcgatc aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa
     1981 ataggcataa atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc
     2041 caactaaact gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa
     2101 aaaagttagt atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga
     2161 aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt
     2221 tggacaataa ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt
     2281 gtgagataag ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa
     2341 ggcaagaaac aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac
     2401 cgaaaaataa tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt
     2461 atataacttt aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag
     2521 ctcaagctag aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag
     2581 gtaatcatta attattgtgg ataataatta acagtgatat ata
//
LOCUS       DADVXN010000193         2620 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.199, whole genome shotgun sequence.
ACCESSION   DADVXN010000193 DADVXN010000000
VERSION     DADVXN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.199"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..477)
                     /locus_tag="KB942_002816"
     CDS             complement(<1..477)
                     /locus_tag="KB942_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBB6853180.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTI"
     gene            553..1119
                     /locus_tag="KB942_002817"
                     /pseudo
     CDS             553..1119
                     /locus_tag="KB942_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1500..1679
                     /locus_tag="KB942_002818"
     CDS             1500..1679
                     /locus_tag="KB942_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853181.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1810..2079
                     /locus_tag="KB942_002819"
     CDS             1810..2079
                     /locus_tag="KB942_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6853182.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            2072..2329
                     /locus_tag="KB942_002820"
     CDS             2072..2329
                     /locus_tag="KB942_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6853183.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          914 a          406 c          507 g          793 t
ORIGIN      
        1 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
       61 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
      121 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
      181 ggttaaacca tctgcatcga tggctcgata caaataatgc cattttcctt taattttgat
      241 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
      301 atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat
      361 gccacgatca tataagattt cttgaacttc acgatagcta aggttataac gaagatagta
      421 gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt
      481 cattcctcct gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt
      541 gcaacagaac ccgaagaaca attgtatttg gaaagtgaaa cgagtcaaac gaaggtcaca
      601 gtcagccata atcgtttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaacg
      661 gagcttctac gactagaaac agaaaaatac atcatgaatg tttacctaga aaggagtgag
      721 ctggtcgaaa actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa
      781 tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc
      841 aaagatgcga cgatagacga ttattacctt gtatttacta gatttttgac gtgtggaacg
      901 gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg
      961 gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt
     1021 ttagacacct atacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga
     1081 atgccatatc aacgatttga aaggaaaaaa aggcaataat catttatacc gaaaagtgag
     1141 aatgtgtgaa ataaggacaa aaaacctagt aactaaggga atggttctta tttcgcacca
     1201 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaac acccgataac
     1261 ctaagagaaa gaaggagatc catatgaata aaatcattcc tctattgtta gtcctatttg
     1321 ttggcgaaat acttatgcgc catgctgcag ctgtgcattt aaacttcgta gtatttttgg
     1381 caatactggt attttgtttg atcttttggc gctttgtatt agcccttgtg agtctactat
     1441 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa
     1501 tgccaattgc aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag
     1561 aattgcagca actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt
     1621 tagatttatt gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac
     1681 gtcacttttt atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata
     1741 ttaatcattt gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg
     1801 gtgaaaggaa tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg
     1861 aaacaagtta atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca
     1921 gttgttgtat tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct
     1981 aataattacg tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt
     2041 aaaacacatg acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg
     2101 ggatgattat ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa
     2161 gttaataaaa gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa
     2221 gcatgattta tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag
     2281 agttgaaaat gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag
     2341 taaggaaagg gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt
     2401 aaaaagctaa tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta
     2461 cggatataag gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta
     2521 aatttcaaca atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta
     2581 gttactaaag gatgacgctt atatatatga ctgaatagaa
//
LOCUS       DADVXN010000194         2594 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.200, whole genome shotgun sequence.
ACCESSION   DADVXN010000194 DADVXN010000000
VERSION     DADVXN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.200"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..1412
                     /locus_tag="KB942_002821"
     CDS             <1..1412
                     /locus_tag="KB942_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6853184.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2594
                     /locus_tag="KB942_002822"
     CDS             1546..>2594
                     /locus_tag="KB942_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6853185.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARL"
BASE COUNT         1018 a          363 c          520 g          693 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tatt
//
LOCUS       DADVXN010000195         2566 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.201, whole genome shotgun sequence.
ACCESSION   DADVXN010000195 DADVXN010000000
VERSION     DADVXN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.201"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..78
                     /locus_tag="KB942_002823"
     CDS             <1..78
                     /locus_tag="KB942_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6853186.1"
                     /translation="LELFFAVFLFLGKNSLKQTKKKELV"
     gene            complement(108..617)
                     /locus_tag="KB942_002824"
     CDS             complement(108..617)
                     /locus_tag="KB942_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBB6853187.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(729..1727)
                     /locus_tag="KB942_002825"
     CDS             complement(729..1727)
                     /locus_tag="KB942_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6853188.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1928..2512
                     /locus_tag="KB942_002826"
     CDS             1928..2512
                     /locus_tag="KB942_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6853189.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          801 a          475 c          483 g          807 t
ORIGIN      
        1 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
       61 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
      121 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
      181 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
      241 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
      301 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
      361 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
      421 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
      481 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
      541 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
      601 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
      661 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
      721 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
      781 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
      841 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
      901 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
      961 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
     1021 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
     1081 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
     1141 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
     1201 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
     1261 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
     1321 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
     1381 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
     1441 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
     1501 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
     1561 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
     1621 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
     1681 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
     1741 ttttcacact atatcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
     1801 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
     1861 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
     1921 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
     1981 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
     2041 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
     2101 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
     2161 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
     2221 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
     2281 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
     2341 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
     2401 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
     2461 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
     2521 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgt
//
LOCUS       DADVXN010000196         2497 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.202, whole genome shotgun sequence.
ACCESSION   DADVXN010000196 DADVXN010000000
VERSION     DADVXN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.202"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            512..607
                     /locus_tag="KB942_002827"
     CDS             512..607
                     /locus_tag="KB942_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6853190.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(909..1154)
                     /locus_tag="KB942_002828"
     CDS             complement(909..1154)
                     /locus_tag="KB942_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853191.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1499..1783
                     /locus_tag="KB942_002829"
     CDS             1499..1783
                     /locus_tag="KB942_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853192.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1786..2202
                     /locus_tag="KB942_002830"
     CDS             1786..2202
                     /locus_tag="KB942_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853193.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          818 a          467 c          407 g          805 t
ORIGIN      
        1 aaaagttgtt agtactcaga taaaaagttg ttagtaccct tctgtatccg ttgggagagt
       61 aaggctcaac tgccccctaa aaagggttct aaaaaatctt taaaaaataa ataaaataaa
      121 cgcgcgcgcg cgagcaaaat ttcccgccaa aaaatcaccc actttttaaa ataaacaggt
      181 tcgcgcatga tattaaactg ccaatctagc tccctatggt cgctgtaaga aggagtgatc
      241 gctacgctct cacattttac ttgcagcagt cgcctttagg ctccttcgcc aaaaataatt
      301 cattagggac tcctttgtgg cgtcctgaga gctcgtctaa gccgttctaa gacaagattc
      361 gataaaaact atgtccttcg ttctaaagcg ccttacaggc ggtcagggca agccctaaca
      421 accccaaggc aataaaaaac ttaggcttat ttttgctttt ttattgtata attgattgtc
      481 gcctatccga cagtcttgcg actggaagga ggtgtgtttc gtgttcacta actttatttg
      541 ctcccttctt gttgggcttt taagcgcttt gttcagttac tggttgaata accgaaacaa
      601 gaagtaaaac ttaattaagg cgactcagcc cacaaaaaaa gccccagaga atccgacctc
      661 tctggggctt tcgtgtgttt cgtgttcact tatttgcaat caaattataa cagaaataaa
      721 tatttatttc aaaaatatcc tacaaaagtc taaaatccgt aattatttct tatatgcaac
      781 atttacaaat aaagtcgctc tgagagctcg tatttttcga tttaagacgt tttatttagt
      841 ttttggtgta tttcattggt ccaagctaaa aaactcttag aagcgatttt agagccattt
      901 aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc tgcgtctgaa
      961 attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt tctttcgggt
     1021 gtcatatttt tatattccaa caatttttcg tctgttaaat cgaacttgat tgcttgaatt
     1081 gcttgctcta atttttctaa gttttgaaaa tattcgtaag gaaatttttc cattttttct
     1141 ttctccagtc tcacaatttt tctagaaata aaatcacttc tttgccgtct atactctcaa
     1201 aaaaaatgtc gtttgaaaaa ctaaataaat ttttgatcgt gcatttttaa atttggttat
     1261 cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac gtgtacccac
     1321 tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa aaaatttgat
     1381 atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa acagctatac
     1441 aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga gattttttat
     1501 ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca aacgttgcca
     1561 agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa aagttttaat
     1621 cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg atttattgct
     1681 cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt atgtctacac
     1741 aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag aaaaacttct
     1801 gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc catcatcgaa
     1861 aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat gagttatgaa
     1921 cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat gggaaaagaa
     1981 atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa agatacaggc
     2041 gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat cgaagctaaa
     2101 ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag tcaaacagtc
     2161 acttctagaa atttcgaccc aacaaatttt ctaaaacgat aaaaaacaga taggagttat
     2221 tcctatctgt tttttattta gaagccccat acaatatgct agaatgtact agcaatctta
     2281 gtcggttgta agggacttgt ctacttgctt aatgtaaggg gggcgagttc ttttttattt
     2341 attcttctaa ccagttacgc agagaaatct ttggcaaatc tgacgttagg cttgaaaaag
     2401 taaattctaa tttggcgatt ttattgccct tttttgcttt tatttttgtg actttaagat
     2461 cattgaagta tttttttagt tctcgtaatg caggatt
//
LOCUS       DADVXN010000197         2436 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.203, whole genome shotgun sequence.
ACCESSION   DADVXN010000197 DADVXN010000000
VERSION     DADVXN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.203"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            133..2286
                     /locus_tag="KB942_002831"
     CDS             133..2286
                     /locus_tag="KB942_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBB6853194.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          427 c          539 g          638 t
ORIGIN      
        1 tgctgtgaaa caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc
       61 cattttttct tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg
      121 gaaggatagg aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa
      181 aaaataagaa aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct
      241 tttcgcttaa attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga
      301 ttgggttatt tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct
      361 acgattacgg tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct
      421 ttagttacga ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct
      481 aaagatttgc ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa
      541 aatctgacag atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa
      601 gccacaaaaa gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg
      661 atggtcaata aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct
      721 accatcttta aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct
      781 ggtgttactg atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt
      841 tcgactggca cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt
      901 ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca
      961 aaaggttatt ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat
     1021 gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg
     1081 aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca
     1141 gaattccaat caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac
     1201 ggcaaagcac agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa
     1261 gtgctagcaa tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct
     1321 ttaggcacga ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca
     1381 gcaggctggg caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt
     1441 ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat
     1501 gcagtcaaag catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg
     1561 ttaggactag actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct
     1621 tatcagcaat tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat
     1681 ttgccgaatg aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg
     1741 gataatggca aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt
     1801 gacacatata caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa
     1861 cgagtacagc cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt
     1921 gaagtgaaga aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat
     1981 atggcgatcc tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca
     2041 gccaaaccat tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg
     2101 gttgcggaag gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca
     2161 acagacaatc cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac
     2221 cacataaact tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca
     2281 aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga
     2341 aaatagcttc tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac
     2401 accgtttact cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DADVXN010000198         2405 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.204, whole genome shotgun sequence.
ACCESSION   DADVXN010000198 DADVXN010000000
VERSION     DADVXN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.204"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(287..649)
                     /locus_tag="KB942_002832"
     CDS             complement(287..649)
                     /locus_tag="KB942_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853195.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="KB942_002833"
     CDS             complement(723..1103)
                     /locus_tag="KB942_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBB6853196.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="KB942_002834"
     CDS             1167..2360
                     /locus_tag="KB942_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6853197.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
BASE COUNT          777 a          443 c          387 g          798 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtc
//
LOCUS       DADVXN010000199         2400 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.205, whole genome shotgun sequence.
ACCESSION   DADVXN010000199 DADVXN010000000
VERSION     DADVXN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.205"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            207..2069
                     /locus_tag="KB942_002835"
     CDS             207..2069
                     /locus_tag="KB942_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6853198.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="KB942_002836"
     CDS             2069..>2400
                     /locus_tag="KB942_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6853199.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DADVXN010000200         2395 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.206, whole genome shotgun sequence.
ACCESSION   DADVXN010000200 DADVXN010000000
VERSION     DADVXN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.206"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            316..570
                     /locus_tag="KB942_002837"
     CDS             316..570
                     /locus_tag="KB942_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6853200.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="KB942_002838"
     CDS             571..888
                     /locus_tag="KB942_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6853201.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="KB942_002839"
     CDS             1447..2382
                     /locus_tag="KB942_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853202.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DADVXN010000201         2393 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.207, whole genome shotgun sequence.
ACCESSION   DADVXN010000201 DADVXN010000000
VERSION     DADVXN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.207"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            116..634
                     /locus_tag="KB942_002840"
     CDS             116..634
                     /locus_tag="KB942_002840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6853203.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            762..1445
                     /locus_tag="KB942_002841"
     CDS             762..1445
                     /locus_tag="KB942_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBB6853204.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1754..1827
                     /locus_tag="KB942_002842"
     tRNA            1754..1827
                     /locus_tag="KB942_002842"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1788..1790,aa:Arg,seq:tct)
     gene            1986..2345
                     /locus_tag="KB942_002843"
     CDS             1986..2345
                     /locus_tag="KB942_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBB6853205.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          819 a          358 c          474 g          742 t
ORIGIN      
        1 atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata atttgaaaaa
       61 atttatcgtt attatatgat taagaaggat tgaataaaga aagaagttga aaacgatacc
      121 tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt taattcttat
      181 tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta ttgaggacaa
      241 agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga aaaatacaaa
      301 tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa aaatctcgac
      361 gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag ctgatacaaa
      421 gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc ccatctttca
      481 gattccatat attggactag tcctttcaaa aacggcattt gcatggattc aggggttatt
      541 ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg tagagttgaa
      601 aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata aatgtattcc
      661 agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca gcttgtagat
      721 ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat gaaatgatgc
      781 atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa aaaaaattac
      841 cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca gttattcccc
      901 atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa cgaatcttgg
      961 agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc ggagaaaacg
     1021 ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa aaaaactatg
     1081 aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca gatatcttgc
     1141 cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca aaatatattg
     1201 aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg gtagatgaca
     1261 tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc aaacgagcta
     1321 tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat ttgacatcga
     1381 caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa acaatcgagt
     1441 tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct caattcgaac
     1501 ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat ctaaataaac
     1561 aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag aacagctgaa
     1621 ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat tcattatttg
     1681 ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa ttattgatgt
     1741 tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct aagcggacgg
     1801 tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag gtttctaacg
     1861 gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa aataaaaaaa
     1921 catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga aaagaggaag
     1981 aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa tttttttata
     2041 tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt tttaaaatga
     2101 gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa ggagtgtaca
     2161 atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca tctaaagaaa
     2221 tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt ttgactagcg
     2281 atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt tctattttct
     2341 tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa ata
//
LOCUS       DADVXN010000202         2294 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.208, whole genome shotgun sequence.
ACCESSION   DADVXN010000202 DADVXN010000000
VERSION     DADVXN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.208"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            131..1744
                     /locus_tag="KB942_002844"
     CDS             131..1744
                     /locus_tag="KB942_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6853206.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          716 a          407 c          447 g          724 t
ORIGIN      
        1 ttaggtttta tccacaaatt atttctctat attttctgtc tattcaaaat aaacaataat
       61 tttccggcac aattatttgc tgttttttac cataacatag tacgatcaac gtagaaaagg
      121 agatgttttt atgaaagttg ttgtcattgg cggtgtagct ggcggtcctt catttgccac
      181 tcgtttccgt cgattgaatg aagcgcacga aatcattatc tatgaacgcg gagaaaatat
      241 ttcttatgca agttgtgctt tgccttatta tttaggaggt gtgatcactg accgtgactc
      301 gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac aacatcgatg tctttactaa
      361 acacgaagta acagcaatcg atccttctac taagcgatta acagttaaag acctatccac
      421 aaatgaagaa ataaaaacag attacgataa gttgatcatc tcttctggtg ctagaccaga
      481 ttatccggat attcccggag tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac
      541 ggatgcggat cgaatcaaat cgttccttga agaaaaaaat ccacaacatg tcgtcattct
      601 tggtgcaggt gttatgggtc tggaattagc tgagaatttc aagcatcgcg gcttagacgt
      661 gactttaatc gatcaattgc cacaagtcgc tttcccttat gatccagaaa ttgctaattt
      721 agtttatgac aaattgctga aagaaggatt agccgttcat ttagaaacaa gagttactga
      781 gatccgtgat aaaggtcgag aaatagtatt atcagatggc tccgtccttt ctgctgatat
      841 gctaattttt gctgttggtg tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca
      901 attatcagat acaggtcaga tcattgttga tgaccagtta caaaccaatc tgccggacat
      961 ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg actggtcagc cgatccaaag
     1021 tatgctttcc agtgcggcca atcgtcaagg acatatgctg gcagatattt taaatggtac
     1081 gcctatgcgc tatcgcggat atattggtgc aggtgtcgca aaaatctttg atcatacagc
     1141 aagttatgcg ggaatgacag aacatgaatt aaaagcatca ggcataacaa attataaaac
     1201 tgtttttatc actccctttg accatgccta tttctatcca gaagctacaa gattaaatct
     1261 aaagctgatt tttgatgcag atagcggtcg tattttaggt ggacaagcat ttggagaaaa
     1321 aggtgtcgat aaacggatgg gagaactttc tgtagcaatc accggaaact tgacagtctt
     1381 tgatttgcct gatttggagt tgccttactc tccaccgtat tctactaccc gtgacccgtt
     1441 gaatatagct ggttatgtcg caatcaatca aatgacgaat atcgtagaaa cgatcaaagc
     1501 aagtgatata cccgaaaacg atttgaaaga agcgttcttt ttagatatac gtgaacctaa
     1561 taaagcacct tctggcagta tctcggcaac aaaaaatatt ccaatgaatg agttacgaga
     1621 tcgaatcaat gaaatcccaa aagataaaaa agtttacatt actttcagaa gaggattgaa
     1681 tacttatact tctgcccgaa tcttggcagg tttgggtatc aaagcgattt tgattgaaga
     1741 ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt tatttttact tgaggagtaa
     1801 aaatgaaaaa gtatataggg ttgttggttg gtatgtatat agaatagaag ataaatgtga
     1861 agaaattgtg cattatttct tcacatttat taggttatag tttaagaaag tttaaacatt
     1921 gcgcaattta attattttag ctgtttcttt tcataaaaaa acatgttgtg actctgagac
     1981 aaatcatagt ccatagtgat gtctctagag taactacgcc taatattacg aggcatcata
     2041 ttaatctatc agcactagta cctaaactag catccctttc gaatattttt tcttatataa
     2101 taaagtttgc tttcactcct ctaatctcac caaatcgact ggcttgcgtt gtgtgatact
     2161 aggaactgat tggtttagaa cgacttttct attgtaatca tatttttcgc catcctcgtt
     2221 gtcttctaat ccttcaatga atgatcccta atgtgtgtat taaataattt aatttttttg
     2281 ttcctattta ttta
//
LOCUS       DADVXN010000203         2145 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.209, whole genome shotgun sequence.
ACCESSION   DADVXN010000203 DADVXN010000000
VERSION     DADVXN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.209"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..337
                     /locus_tag="KB942_002845"
     CDS             <1..337
                     /locus_tag="KB942_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBB6853207.1"
                     /translation="TDLGNKTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRL
                     HKGICTAAFRANNLSCMIYGIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPH
                     NLEHLVLQH"
     gene            428..1708
                     /locus_tag="KB942_002846"
     CDS             428..1708
                     /locus_tag="KB942_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBB6853208.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDINLLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1780..>2145
                     /locus_tag="KB942_002847"
     CDS             1780..>2145
                     /locus_tag="KB942_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6853209.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYF"
BASE COUNT          931 a          252 c          326 g          636 t
ORIGIN      
        1 aactgattta gggaataaaa cattaggaca aacgatacac aataaaagat gttatgtgat
       61 attaattaat caaaacctat caccttctat gaaaagattt gtattatggc atgaaatagc
      121 ccacgttaga ctccataaag gtatttgcac agcagctttt agagcaaaca accttagttg
      181 tatgatttat ggtatagaag ctgaagctaa tatgtttgca attgaaatgt taactagaac
      241 aatagatgaa caagatttat ctgatatgag tcagttccaa attattgaat atttagggct
      301 tcctcataat cttgaacatt tagtattaca acattaaaaa taaaaaagcc catgctttca
      361 catggactct ttcctcattt ctgagtaaac tctacaataa ttatatcaaa gaaatgagga
      421 gttttgaatg ttattattga ttggacttat tttattaatt ataggaatca ctaaactgtc
      481 gaaagctaac acaataaaaa aagaagctta tgaattagaa caaaaaaatg atgaaaaact
      541 aaataaatta catgatagag aaagatttat attaagtaag gaagaaacac tatcagattt
      601 aaataaacaa attactatgt taaaaaatga tattaattta ttaaaaaaag agaaagaaga
      661 tttaaatgat gaaataaatc aaaagtatga tgaaacatta aaaaatgtct attcttcact
      721 tatcgtttca aatatagaag aaacttcatc ttctgaaata aaaaataagc tttctttgtt
      781 aaaattaaaa gaaaaggaat atattattga cgatgctgtt tctgtccctc ttattttaaa
      841 taaacgagag aaaaataatt taaagaaaaa aatgctcatt ccatttgatt cagaagtgaa
      901 aggattactc aataaactaa ctatttctaa tatagactcg actagggaaa aaatcataaa
      961 aatatttgat aaaattaata atttattcaa gaatgacaat gctgaattaa ggaagggtct
     1021 tttagagttt aaacttcaag aactagaact tcattactct tatttagtga aaattaccga
     1081 agaaaaagaa caacaaaaag caataaaaga acagatgata gaagaggaaa aagttcgtag
     1141 agaaattgat cgtgaaaaga aacgtattga taaagaacaa cgtcaattca attctgaaat
     1201 ttccaaatta atcgcatata tgcaaaaagc taatgcagat gtggaaaaag aattatatgc
     1261 aaataaaatt caagaactgg aagaaaaatt aaaagaatta gaagttatac gtgaaaatgt
     1321 attacaaaga gaattaaata ctagagccgg ttatgtgtat gtaattagta atattgggtc
     1381 ttttggtgaa gacatatata aaattggtat gactagaaga ttagaaccta tggatagaat
     1441 aaaagaattg agtagtgcat cggttccatt tgaatttgac gttcatgcga tgattttttc
     1501 agaggacgct ccgtctttgg aaactaaatt gcataatcat tttagaaaac aagaagttaa
     1561 taaaattaat caaagaaaag aatttttcaa agtttcacta gacgaaatag agaaagttgt
     1621 tttagaaaat tataatggaa cagtaacatt tacaaaatta gctaaagctg aacaatatag
     1681 acgatcttta gaattgtcaa aagattaaat atataaaaag ccttcgggct ttttctttta
     1741 acaattaata ggaacgtata ttcgaaagga atagaaataa tgtggttaga aaaaataaat
     1801 gacaaaaaat ttaagtacac cgaacgttat acggatccct taactgaaaa aaagagaaaa
     1861 gtgtccgtta ctttatctag caattcacgc caagcttgga atcaagctaa cttacttttg
     1921 aatgaaaaaa tcgcagaaaa aataaaacga aatgaagaat taccactaac ttttggagaa
     1981 ttaaaaacta aatgggatga aaaatacaag ccaactgtaa aagaaagttc ctatcgaaca
     2041 actcaggttt atctatcatt aatttcaaaa tatataaaag atgatgtact tgtaaaaaac
     2101 gttaatagta atcttataca agatatgcta gattactact atttt
//
LOCUS       DADVXN010000204         2131 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.210, whole genome shotgun sequence.
ACCESSION   DADVXN010000204 DADVXN010000000
VERSION     DADVXN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.210"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            16..>2131
                     /gene="acm"
                     /locus_tag="KB942_002848"
     CDS             16..>2131
                     /gene="acm"
                     /locus_tag="KB942_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBB6853210.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          839 a          343 c          416 g          533 t
ORIGIN      
        1 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
       61 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      121 agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      181 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      241 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      301 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      361 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      421 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      481 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      541 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      601 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      661 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      721 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
      781 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
      841 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
      901 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
      961 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1021 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1081 agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1141 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1201 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1261 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1321 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1381 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1441 aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa
     1501 tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat
     1561 caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca
     1621 ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca
     1681 gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat
     1741 tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact
     1801 gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac
     1861 agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa
     1921 aattttccag agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa
     1981 atatttgctc atacaggaga atccagcgaa caaagaagca attatattag taagagagct
     2041 gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa
     2101 tctagttttg ctgtaatcat tggtttattg a
//
LOCUS       DADVXN010000205         2111 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.211, whole genome shotgun sequence.
ACCESSION   DADVXN010000205 DADVXN010000000
VERSION     DADVXN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.211"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..169
                     /locus_tag="KB942_002849"
     CDS             <1..169
                     /locus_tag="KB942_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6853211.1"
                     /translation="NKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYH
                     ANEVLKIAEEK"
     gene            170..922
                     /locus_tag="KB942_002850"
     CDS             170..922
                     /locus_tag="KB942_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBB6853212.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            927..1874
                     /locus_tag="KB942_002851"
     CDS             927..1874
                     /locus_tag="KB942_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBB6853213.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          738 a          279 c          421 g          673 t
ORIGIN      
        1 taataaaata agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat
       61 gtatgagaag gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa
      121 tgatttatat catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt
      181 atttactatt tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt
      241 ggcaggaaag cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca
      301 tcctgaacat aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac
      361 gactaagtta aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac
      421 tgtagggaaa attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat
      481 atttgattta attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatcttga
      541 agaattaata gatgaacatc taaaaaattt agataaagac gtctactttt cagttgtttc
      601 tgcaagacgt aatccctatt ttaatatggt aaaaagaatt gaaaatgaag ttactatagt
      661 taataaatca agatacacat ctaggcaaca gacacctgaa gtttttgaaa tgaatgcctc
      721 aatgtattct tttaaacctg aatttctaaa aaaaagtaat tatatttttg atgggcaatg
      781 tgggataata aaaatgagag attatctaat tttagatatt gatagtgaag aagattttaa
      841 atggttagaa tttttgtttc ctaaattttt agaagattct tcggagcttc aagatatcta
      901 taattattta tatcggaggt aaaaggatga gctgttatct tatcaccggt ggggctggct
      961 tcattggttc taccttagcc aatcatttag gagaagaaca tgtggtaatc gtcgtcgatg
     1021 acttatcaat gggaaaagta gaaaatcttg atatgacgaa aaatatcact tttattgagg
     1081 gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa agctaataaa tttgattata
     1141 tctttcattt agcagcagtt gctagtgtgg cagattcagt tgcccgacca gtagaaacac
     1201 atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt gatccgcaag tatcaacctg
     1261 atttaaaacg gatcgtgttt agttcttcag cggctgttta tggggatgaa ccgactttac
     1321 caaaaaaaga agagtccgtg attcgcccat tgacgcctta tgcggttgat aaattcgctg
     1381 cagaacaata tgtgctagat tattgtcatt tatatgatgt acccggtagc gcagttcgtt
     1441 ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc atacagtggt gtgatttcta
     1501 ttctcgtaga tcgctataag aaacaaatcg taggagaagc aacttcattt acattatttg
     1561 gtgatggaag ccagtctaga gactttgttt atatagatga tgtaatccaa gcattacttt
     1621 tagttgcgaa tgaggaagct gcactgggga aacaattcaa tgttggaacg ggtaaagcaa
     1681 ccacactttt ggaattgatt catgcaatca atgaaattct cggtgtagaa ttgacacttg
     1741 aatacaaaga agagcgttcg ggtgatatcc atgactctct agcagatatt actaagatcc
     1801 agtcaattgg ctatcaaccg aaatatggta ttttaagtgg aatgactaat tatttgaaaa
     1861 ctgaaatcaa ttaaaacata ctaagataag attagagggt aaattcaggc tctttgtcaa
     1921 taaggactga tgagttttgt aaaatcaaat ctgggataga agggctcttt gtcaaatgat
     1981 gttggtaaag agaaatctta gaataatttg gacagaacag aaagaagaaa acctctttct
     2041 gttctgttta tttggttaaa aatgttttgc catgacgaat cataattcgt agtttgaaat
     2101 tgtaaaagtt g
//
LOCUS       DADVXN010000206         2043 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.212, whole genome shotgun sequence.
ACCESSION   DADVXN010000206 DADVXN010000000
VERSION     DADVXN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.212"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            656..988
                     /locus_tag="KB942_002852"
     CDS             656..988
                     /locus_tag="KB942_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBB6853214.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1607..1948
                     /locus_tag="KB942_002853"
     CDS             1607..1948
                     /locus_tag="KB942_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBB6853215.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          713 a          271 c          430 g          629 t
ORIGIN      
        1 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
       61 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
      121 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
      181 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
      241 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
      301 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
      361 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
      421 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
      481 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
      541 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
      601 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
      661 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
      721 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
      781 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
      841 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
      901 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
      961 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     1021 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     1081 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     1141 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     1201 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     1261 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     1321 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     1381 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     1441 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     1501 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     1561 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     1621 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     1681 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     1741 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     1801 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     1861 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     1921 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     1981 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     2041 ttt
//
LOCUS       DADVXN010000207         2039 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.213, whole genome shotgun sequence.
ACCESSION   DADVXN010000207 DADVXN010000000
VERSION     DADVXN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.213"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..607
                     /locus_tag="KB942_002854"
     CDS             <1..607
                     /locus_tag="KB942_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBB6853216.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="KB942_002855"
     CDS             620..1528
                     /locus_tag="KB942_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6853217.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..>2039
                     /locus_tag="KB942_002856"
     CDS             1528..>2039
                     /locus_tag="KB942_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853218.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          680 a          298 c          489 g          572 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgt
//
LOCUS       DADVXN010000208         1976 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.214, whole genome shotgun sequence.
ACCESSION   DADVXN010000208 DADVXN010000000
VERSION     DADVXN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.214"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..425)
                     /locus_tag="KB942_002857"
     CDS             complement(<1..425)
                     /locus_tag="KB942_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6853219.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="KB942_002858"
     CDS             complement(534..1565)
                     /locus_tag="KB942_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6853220.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          609 a          326 c          341 g          700 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agattt
//
LOCUS       DADVXN010000209         1945 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.215, whole genome shotgun sequence.
ACCESSION   DADVXN010000209 DADVXN010000000
VERSION     DADVXN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.215"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..519)
                     /locus_tag="KB942_002859"
     CDS             complement(<1..519)
                     /locus_tag="KB942_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBB6853221.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQN"
     gene            1213..1599
                     /locus_tag="KB942_002860"
     CDS             1213..1599
                     /locus_tag="KB942_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6853222.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1693..1884
                     /locus_tag="KB942_002861"
     CDS             1693..1884
                     /locus_tag="KB942_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853223.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          724 a          289 c          262 g          670 t
ORIGIN      
        1 attttgtgat aacagattat ctattttgat ttgctcgata gttttactta gattttctgt
       61 atattcaatt aacggttcat ggaatagatc ttctattttt ccagtaaaaa aataatgaac
      121 taaaatcaga atatctcgaa tgtctcctaa agaatacaaa gtcaaatttt gcaatgaatt
      181 atagagtcgt tctctatcag ccattgcaac attcaaattg ttcattttta gtttaaaaaa
      241 aactttatca atattatttg ctctaaatgg accaaataag taatatctgg ttttaaacat
      301 gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt tagttttttt
      361 aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc tatctgagcg
      421 atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact ttaaacaaac
      481 atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc tctttccttt
      541 ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg tcaaaataag
      601 ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc accgtaaaaa
      661 gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat ttacatatat
      721 ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata cagataatag
      781 gaatttttcg tttctaaata ttccaatttg ggaatccaat aaaaattttc gttcaaatac
      841 gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat aaaaaataca
      901 atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt tattatacgt
      961 aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag aagatatatg
     1021 aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa catttttgaa
     1081 aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc gcagcgcagc
     1141 ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat cttaatggag
     1201 gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat tcgattcttt
     1261 gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga aattcaaaaa
     1321 catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat aaaatttaaa
     1381 caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt agcaaaaatc
     1441 cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat ctttgataaa
     1501 actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac aaatttaaaa
     1561 catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa taactaattt
     1621 tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag tccatgggaa
     1681 gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc tggaaatgaa
     1741 gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca atcctggaga
     1801 actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata tctagctatc
     1861 tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa gtaaaaagat
     1921 agtttcaaat ttcgaacaat aaatt
//
LOCUS       DADVXN010000210         1942 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.216, whole genome shotgun sequence.
ACCESSION   DADVXN010000210 DADVXN010000000
VERSION     DADVXN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.216"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            89..1555
                     /locus_tag="KB942_002862"
     CDS             89..1555
                     /locus_tag="KB942_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6853224.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          599 a          333 c          397 g          613 t
ORIGIN      
        1 acataaaata acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa
       61 caaattttta tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt
      121 tgcgttaatt gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca
      181 gcaaggagta tcagttgtca cctcttggct attgattttg gccatttatt ttattccata
      241 cgcattgatc gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc
      301 atgggtcgaa aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg
      361 ggtcgtccat attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg
      421 ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat
      481 ttcacttatt atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa
      541 agtgattggc ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt
      601 agctgtaacg gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca
      661 agtgaaaaca tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt
      721 atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc
      781 aaaggaattt cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt
      841 aggctcagtc gcaatgggta tgctgtttgc tagtgataat attcctaaag acttgatggc
      901 aaatggggca tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt
      961 gatgatcatt tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat
     1021 tgatgcacca ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct
     1081 acgtaaacgt aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt
     1141 aggaatcatt attttgttgc cgatttttgg tatcaaagat atgaatgaat tagtaaaatg
     1201 gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata
     1261 tatgatgttg aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc
     1321 aaaaatcggt tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct
     1381 aggaatggtg ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt
     1441 tacgaatatc atcacaccaa tcgtcttgat cttattaggt atgatcttgc cagcaattgc
     1501 tcgtcgagac aaaaacaaag aaatcactga accagaaaca gtggttcagc cttaaaatag
     1561 aaatacttgt tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt
     1621 tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt gtttttcgaa gaagctactt
     1681 ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
     1741 ccaaacaaaa atagcccctc atatttttcg tttggctggg cttattaccg cagatgcaag
     1801 ctttggaaca ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag
     1861 aaacaaacgg aaaaatagct cctcatattt tgccgctttt tgagctaatg ctgaaggtca
     1921 gctgattgaa caccatttat tc
//
LOCUS       DADVXN010000211         1917 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.217, whole genome shotgun sequence.
ACCESSION   DADVXN010000211 DADVXN010000000
VERSION     DADVXN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.217"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..435
                     /locus_tag="KB942_002863"
     CDS             <1..435
                     /locus_tag="KB942_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6853225.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="KB942_002864"
     tRNA            complement(759..842)
                     /locus_tag="KB942_002864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="KB942_002865"
     CDS             1370..1870
                     /locus_tag="KB942_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6853226.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          619 a          362 c          328 g          608 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaat
//
LOCUS       DADVXN010000212         1907 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.218, whole genome shotgun sequence.
ACCESSION   DADVXN010000212 DADVXN010000000
VERSION     DADVXN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.218"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(1..86)
                     /locus_tag="KB942_002866"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="KB942_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(351..806)
                     /gene="tnpA"
                     /locus_tag="KB942_002867"
     CDS             complement(351..806)
                     /gene="tnpA"
                     /locus_tag="KB942_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBB6853227.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1049..1907
                     /locus_tag="KB942_002868"
                     /pseudo
     CDS             1049..1907
                     /locus_tag="KB942_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          607 a          371 c          357 g          572 t
ORIGIN      
        1 gacagatcat caatacttac gtaactcaaa aatacttcca aaggtgttcg ataatccagt
       61 gattttctag gaagattatt tctgcgccag cattttctaa tgtttgaatt tgtgttttgt
      121 aatcttggtt gcttgtgcta attcttgaat agccgtaaat tgccaaaatt tcatcaccct
      181 ttctggacat aagtttctga caccagttaa aatcaagtct atcagtaacc catacacccg
      241 tgaggacggg tgtattttta atccccctag aaggggtcga cactcgctgc cccttaaaag
      301 gggattttta aaagaccgcc aactgctcat cttcactcac ttacttctct ttatttttta
      361 aatggttcta cgtactctct tttacttata atatatcgaa tgcgatcatc agcttcttgt
      421 tcacggatgt atttttcatt tgtcttttga ttcaatccga ctgtactcac atagtatctc
      481 ttcgaccaaa aggatttgtt tccgtattta tatttgagat ttgcgtgctg ctcgtggatt
      541 agtaccgcac tacgaccttt cagatacccc ataaaactgg atatacatat ttttggtggt
      601 actcgaacaa gcatgtgaat atgatctagc attgcatgtg cttctatgat ttccacatct
      661 ttcatttcgc acaacttcct aagtattttt ccaagatctt ttcgtaattg cccatagatt
      721 acttttcgtt gatattttgg tgtaaaaata atatgatact tacaattcca acgagtatat
      781 gctaaacttt tatcgtcatt tgacatgaaa aaactccttt cgatctgatg agcggttggt
      841 agccactcac tagtatatcg aaaggagttt ttatcgctaa agatacttct tccacgcgta
      901 gaaacgcgcg gttttcttgt ttcgcacgct ttacgcacaa aactgactga agtcattaag
      961 aaaataaaag ggtctaaaac ttttaagttt tgtaccgacc cccaaaagtt agaccaaaaa
     1021 atctaacttt tggaggtcgg tattttttat ggctaagtat acttttgaac ttaaattaaa
     1081 gatcgttcat gattacttgg atggtaaagg cggaagtgat tatctggcaa agaaatattc
     1141 cattaaggct ccatcacaag tgaagcgctg gatcaatgcc tatcaagaat ttggtgaaga
     1201 gggtctagta agaaagcgac aaaagaaaaa atattctgtt caatttaagc tggatgcgat
     1261 agagttatat ctaacaagtg agctgtccta tcgtgaagtt gccaacacac ttaacatgaa
     1321 caaccctaat ttgatcgcta gttggatgcg tcaattccgt gagggtggga tcgatggact
     1381 ctcaaaaacg aaaggatgtc cacctatctt gtctaagaaa aatgaaccca aaaataagaa
     1441 aaaatctaca actaaagcaa cgtctaaaga acgtgaacgc atcgaggagt tggagaaacg
     1501 agtccgttcc cttcaaattg aaaatgcctt tttaaaagaa ttgaggaagc tgcgaaaaca
     1561 ggaagcccaa caacgacgaa tgaagcaatc gcacgaatca tcgcaagcct ccgaggaccg
     1621 tttaaattag ttgagcttct agaaacttta aagttcccta aggcaatctt tatgtactgg
     1681 caaaaacgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaagtt
     1741 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
     1801 ttctctatca ataaaaagaa agttcagcgc ctgatcaaga agctaggaat aaaagtaacg
     1861 acatacacac gtaaatcacg gcgctacaat tcttatcgag gacaagt
//
LOCUS       DADVXN010000213         1846 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.219, whole genome shotgun sequence.
ACCESSION   DADVXN010000213 DADVXN010000000
VERSION     DADVXN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.219"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            76..1641
                     /gene="guaA"
                     /locus_tag="KB942_002869"
     CDS             76..1641
                     /gene="guaA"
                     /locus_tag="KB942_002869"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6853228.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          565 a          381 c          390 g          510 t
ORIGIN      
        1 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
       61 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagccaaa aattatcgta
      121 cttgattacg gtagtcagta taaccaactg atcactcgcc gtattcgtga gttcggtgtt
      181 ttctcagagt tgatgagcca ccgcattacc gcagaagaag taaaggcaat gaaccctaaa
      241 ggaatcatcc tttctggcgg accaaacagt gtttatgatg agaaggcctt tggtattgat
      301 gaaaagattt ttgatttggg gatcccagtt ttaggaatct gttacggtat gcagttgatt
      361 acttataaat taggcggaaa agttaaacca gccaaaaacc gtgaatacgg aaaagcagaa
      421 ttggaaatca cttctgcaga agcggccctt ttccaaaaca ctcctgaaaa acaaaccgtt
      481 tggatgagtc atggtgacct agttacccaa gcaccgactg atttcgatgt cgttgctacc
      541 agcaaagatt gccccatcgc atcgatccaa aatgcagaac gcaaaatgta cggaatccaa
      601 ttccatgcag aagttcgcca ttccgaatat ggaaatgact tgttgcgcca ttttacacta
      661 gatgtctgcc aatgtactgg tgactggaca atggataact tcatcgacat ggagatcgca
      721 aaaattcgtg aacaagtcgg tgacaaaaaa gttttattag gcctttcagg cggagtagat
      781 tcctctgttg ttggtgttct tttacaaaaa gcgatcggcg atcaattaac atgtatcttc
      841 gttgatcacg tattgttgcg taaaggtgaa gcagaacaag ttatggaaag tcttggcgga
      901 aaatttggct tgaacattat aaaagtcgat gccaaagaac gtttcttatc aaaacttgct
      961 ggcgtgtctg atccagaaga aaaacgtaaa atcattggaa atgaatttgt atatgtcttc
     1021 gatgatgaag caaccaaact tgccggtgaa gaaggcgttt ctttccttgc acaaggaacc
     1081 ctttataccg atgtgatcga atcaggaact gaaactgccc aaaccatcaa atcacatcac
     1141 aatgtaggtg gattacctga agacatgcaa ttcaagctga tcgagccatt gaacacattg
     1201 ttcaaagacg aagtacgtga attaggtacc caattaggca tgcctgacag catcgtttgg
     1261 cgccaaccat tccctggacc aggcttaggt attcgtgtcc ttggtgaatt gacagaagaa
     1321 aaattagaaa tcgtccgtga atcagacgct atcttgcgcg aagaaatcgc caatgccggt
     1381 ttagatcgcg acatctggca atacttcacc gtcctaccag gcattcgctc tgttggggtt
     1441 atgggagatg gccgtacgta tgactacact gtaggtatcc gtgctgtcac ttctattgac
     1501 ggcatgaccg ctgactttgc ccgtatccct tgggaagtct tgcaaaaaat cagtgtccgt
     1561 atcgtaaatg aagtcgcaca cgttaaccgt atcgtgtatg atattaccag caaaccacct
     1621 gcaacagtgg agtgggaata aagagccaac ataatatgga tgaggaatac ctataaatca
     1681 acattattca attactaaag ttcctgattt tcgtaaaaaa taagatggtt tcctaccatt
     1741 ttccctacca aagcccttgt tagattaact actaacaagg gctttgcttt tattcactac
     1801 ctttattttc ctattgaatt agctttttat ctaatcagat ttttag
//
LOCUS       DADVXN010000214         1818 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.221, whole genome shotgun sequence.
ACCESSION   DADVXN010000214 DADVXN010000000
VERSION     DADVXN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.221"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            57..197
                     /locus_tag="KB942_002870"
     CDS             57..197
                     /locus_tag="KB942_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853229.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            495..752
                     /locus_tag="KB942_002871"
     CDS             495..752
                     /locus_tag="KB942_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBB6853230.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(723..1031)
                     /locus_tag="KB942_002872"
     CDS             complement(723..1031)
                     /locus_tag="KB942_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853231.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1109..>1818
                     /locus_tag="KB942_002873"
     CDS             1109..>1818
                     /locus_tag="KB942_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBB6853232.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEG"
BASE COUNT          673 a          315 c          345 g          485 t
ORIGIN      
        1 gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag aagaatatgg
       61 aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa caagcaaaag
      121 tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc atagcagaga
      181 ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca tagataacag
      241 cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat tcgcctttag
      301 tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg atttcaaacg
      361 gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt cgtggtaata
      421 ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa tcatttatta
      481 aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa aaaatgatag
      541 atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa ttgcaaggac
      601 aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg aatccaacaa
      661 aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac gagcaagaat
      721 tactatttga aaccgatact tttaacagct gatgttttat agatgacagt ttcatccgaa
      781 tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat aagacctaca
      841 agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt attcacgtaa
      901 atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc atcgtatcca
      961 acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt aattacaaat
     1021 tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca ctgataagga
     1081 aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta atttcgagca
     1141 aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa aagatgtagc
     1201 agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg acttggagga
     1261 taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga ttaacgagtc
     1321 aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg
     1381 ggtaacgagt gaagtccttc caacaattag aaaaacaggt agctattcaa acgtacctca
     1441 aagttttgca caagcattgc gtttagcagc ggatttagaa gaaaagaacc aattactcga
     1501 acaacaaatt gccgagtacg aaccaaagat tagctactta gatacgattc tttcatcgac
     1561 agatacggta gctacttctc aaattgcagc tgattacgga atgtcggcaa ttgctctaaa
     1621 caaattgcta aacgagttag gtgttcagca taaagttagc ggacaatgga tactttaccg
     1681 aaaacatatg aaccaaggat acacaaaatc gcacacaagt gagataccga aagccgatgg
     1741 cggcactaaa gttgtaatga ataccaaatg gacacagaaa gggcgagtgt ttatttacaa
     1801 cttattaatc gcagaggg
//
LOCUS       DADVXN010000215         1754 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.222, whole genome shotgun sequence.
ACCESSION   DADVXN010000215 DADVXN010000000
VERSION     DADVXN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.222"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..572
                     /locus_tag="KB942_002874"
     CDS             <1..572
                     /locus_tag="KB942_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853233.1"
                     /translation="LYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQP
                     LLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLE
                     NDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFC
                     EHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            559..>1754
                     /locus_tag="KB942_002875"
     CDS             559..>1754
                     /locus_tag="KB942_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBB6853234.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLL"
BASE COUNT          580 a          227 c          279 g          668 t
ORIGIN      
        1 tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga
       61 tgaaaaacat acaatcaatc tttgggatag aagaagtttt tgtcggagat aaatgtatct
      121 tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat attttcgcta
      181 aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat cctagagcaa
      241 ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt gaagtcttgc
      301 tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca atttcttttt
      361 caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa ttaattttaa
      421 atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt gttcttttct
      481 gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac tgggcagaat
      541 taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa attagttaag
      601 tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt cataactatt
      661 cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa tttcacttca
      721 tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat taataatgca
      781 agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc tctagctaca
      841 ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc aaatgctttt
      901 tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc tagttttgta
      961 attagttata tccagtctta ttttacgatt aactttaacc attttaaata tttgatttta
     1021 tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca gacattatat
     1081 tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt gttgataggt
     1141 ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa aaaacagtgg
     1201 agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa tatagtttta
     1261 gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg gggaatatat
     1321 tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac taataacgcc
     1381 tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt
     1441 agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt agtttcgatt
     1501 gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt agtggttccg
     1561 ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc tgaattttat
     1621 gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt taatatacta
     1681 ttaaacatga ttttcatacc taggttagga ataattgctg ctggatacac aaccgtattt
     1741 acgtattttt tact
//
LOCUS       DADVXN010000216         1742 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.223, whole genome shotgun sequence.
ACCESSION   DADVXN010000216 DADVXN010000000
VERSION     DADVXN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.223"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..913)
                     /locus_tag="KB942_002876"
     CDS             complement(<1..913)
                     /locus_tag="KB942_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBB6853235.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNE"
     gene            complement(1072..1338)
                     /locus_tag="KB942_002877"
     CDS             complement(1072..1338)
                     /locus_tag="KB942_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853236.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1328..1684)
                     /locus_tag="KB942_002878"
     CDS             complement(1328..1684)
                     /locus_tag="KB942_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6853237.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          504 a          331 c          207 g          700 t
ORIGIN      
        1 tttcattttt taaaaaatac cttttaaatt tcttatagtc ctctgaaatc aaaatatagc
       61 gtgatggata ctcatcaaaa gaagaatcgt attcttcctt taaaattccc gatttactta
      121 atttttctag tacatcaaat actctatcac tcaagtaact ccttaaattg ttatcacttt
      181 cccattgaac tacttttcta taagttgaat tatcagttga aaagatagaa ttttcatcca
      241 taaacgcata tattagcagc aatctttcat cattgtcaaa ttctattgga tcaaaaatat
      301 ctaagttgtc atcaaaatct ttatcagttc cggtaacttt tttgttaatt actgaaggtg
      361 tcttgtaaga aatagaatct aaatcaaagc gttttgattc taaattattt tgtgctttct
      421 caattaaata attcactttt tttgtgagaa accttctgat attgtctttg ctatatgttc
      481 cataaaaacc cttatcttct aaagagtccc tgtacttctt aacttctata tactcttcag
      541 ccaaaacttg tggaactggt tcatctaaga aaaaaacccc tacctcgcct ttgctatttt
      601 tataaaagta atcaatttct gctatagttc ccgatctata attatcaact ggagaaccca
      661 acttatggcc aaaaatcgct aataatatat cactattttc tacaatttgt ttattaataa
      721 cactttgccc agagctatta tttatataag taggaatcgc attcgtttcc catcgaattg
      781 gcatcaaaat tacttttgta ttatctgtat tttctatatt ccatctatat atggtttctt
      841 ctacttcatt cctctttgct gctatatcac ctggtgatgc tataaatact tttattagat
      901 ttatggtttt catttattgt acctttctct ttatgtctct ttaaagtaat ttttgtataa
      961 aatttattga cttttacact ataggacaac ttttatctat ttagtacaaa aaggcccact
     1021 aaactaattg attagttagg agcctattac aggtatattt tcaactcgct tttaagcaaa
     1081 ttctactacc agctttttgc ctaatgccct agcgatatca tcaagtttat cgaaagatac
     1141 gttatcccct ctctcaattc gagcaatagt agattgagtt gtatgtgctt tttgtgcaag
     1201 ctccgcttga gttaggcctg cttcctcccg tgcatgataa agagcaaccg cagtttcgag
     1261 ttttcttcct tcacggtcat atgcttctgc aaaatctttg tctttttgct tttcttcaat
     1321 caatttttta atcatcattt gtgaaccact cctctctaag ttcttttgca tgtttaagtt
     1381 cacttattgg tgttttctcc gtttttttag taaatccatg ggtaatgaca taccttccat
     1441 tgaccacatg gaaatagata gctctttgga tgtttgatcc taccttactt cttaattcgt
     1501 acaaattagt gtctaatttt ttcacccatt tcaaccgttg agctactagc atcccattct
     1561 tttctgtttc ttctattgta cgtaaaagct tcgcactatc tttttttggt aatgtttcca
     1621 accattctaa aaattcatca tgaccattag gtcttttaaa tgtttcaaaa gatatttttt
     1681 ccatacccaa ttatatcata tattgatttt atattatagt atataacata aaaattcgaa
     1741 tt
//
LOCUS       DADVXN010000217         1741 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.224, whole genome shotgun sequence.
ACCESSION   DADVXN010000217 DADVXN010000000
VERSION     DADVXN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.224"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            49..1572
                     /locus_tag="KB942_002879"
     CDS             49..1572
                     /locus_tag="KB942_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6853238.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          628 a          279 c          352 g          482 t
ORIGIN      
        1 atcaatcgta tgatcgatca aatggagtct gtcaaaaaag aaagttattt ggaccctact
       61 gtttttgaag cagaggaaca tatccaaaaa gtcattcata aaatgttccg tccagacaat
      121 gcctatacaa aatttgattt tcgaaatggc cgtccaacga agtctgcgat cttaaccacg
      181 agttccattg atatggccaa aaagtattat cgggccatca aagagatgac gaaagaatct
      241 gattggttaa taaaagagtt tcccaatcag cctatccgtg aaggacgaac gatggaggat
      301 cctgatttcc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      361 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatactgcc
      421 tggtcactag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      481 agagcggaat tcaaacaatt tgggaaacaa atcgatttag taatcgtagt ggatcgtcta
      541 ttgacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      601 gcaggattga tccaagcctt ttcacgtacg aaccgtacgt atcctgaaaa aacaaaagga
      661 ctaattgtta ctttccgaaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
      721 ctgtattcag aagctaaaga agaatcgggt ttgatttatc caagttacca agaatccaaa
      781 aaaagattca agcaagcata tggtaagtta aaagaggttt catccatgga atccattgat
      841 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
      901 gcgtatgaat cgttagtgac ttatgatgat tacaatgatg acatggtgca atcttcaact
      961 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1021 tcattgatgg aggaagaacc agaaaaagag ggagaagatt tttcaacgat cgaattttat
     1081 agtgaaaata ccactaagtt gtatgatatt gattctactt atattgatca attactagga
     1141 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1201 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1261 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcaagt
     1321 gataatttga ttcatgcctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1381 tctaatctgc aatatatgcc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1441 agtaaggatt acgaaggata taaagccaaa catccagaag cgaaaccatt taaataccca
     1501 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgaaaat
     1561 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatag tagtaaagtg
     1621 gaagtgtaga aaatagggat agatgtcttt ttacttgttc acattttata acggagtatt
     1681 taagaaagct attgaataat gattcttcca tctttttcat tcatttaccg gttctgttgc
     1741 a
//
LOCUS       DADVXN010000218         1612 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.225, whole genome shotgun sequence.
ACCESSION   DADVXN010000218 DADVXN010000000
VERSION     DADVXN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.225"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..1376
                     /locus_tag="KB942_002880"
     CDS             <1..1376
                     /locus_tag="KB942_002880"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBB6853239.1"
                     /translation="EESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQLNDLNK
                     AFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIG
                     DAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLM
                     LNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDE
                     PYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGT
                     MAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQT
                     RDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLANFDEIKENDYNL
                     NIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGA
                     LEVFKHE"
     gene            1369..>1612
                     /locus_tag="KB942_002881"
     CDS             1369..>1612
                     /locus_tag="KB942_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6853240.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          564 a          270 c          341 g          437 t
ORIGIN      
        1 atgaagaatc aaaagaagat ctgattgcaa caatcgttga tattttgggg tatgcgattt
       61 cgccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga
      121 atgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat
      181 ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga
      241 cgattaccga agtcattaag aaattgaatg acgtggaagt tttaggtcac gatggggacg
      301 tgatcgggga tgcttatgaa tttctgatca gtcaatttgc ttcagaagca gggaagaaag
      361 ccggagaatt ttacacgcct catatggttt ctgatatgat ggcacaaatc gtgacgttgg
      421 atcaaaaaga acgacgattc tttagtgttt ttgatccaac catgggttca ggttcattga
      481 tgttaaatgt gcgtaactac ttaactcacc cagataacgt taaatatcat gggcaagaat
      541 taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggg gtagatgccg
      601 aagaaatgaa tctccgcaat ggggatacgt tgaataagga ttggccaaca gatgaacctt
      661 acacatttga tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga
      721 ccttcttaga cgattctcgg tttaatcgtt atggaaaatt agcgccaaaa tccaaagcag
      781 attttgcgtt tcttcttcat ggtttctatc atttgaagga aacagggact atggccatcg
      841 tcttgccaca cggagtttta ttccgtggcg ctgcagaagg ggtcattcgt caaaaattac
      901 tagaagacgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc ttcgggacat
      961 cgattccaac gacggtgatt gtattgaaaa agaaccgcca aactcgagac gtattattca
     1021 ttgacgctag tcgagaattt gtcaaaggaa aaaatcagaa taaattatcg gaagaaaaca
     1081 ttcaaaaaat cctcgaaacc tatgctgaaa gaaaagatgt cgagaaatac gctcatttgg
     1141 caaactttga tgaaatcaaa gaaaatgact ataacttaaa tattccacgc tatgtagata
     1201 cgtttgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc aagaagatac
     1261 gacaagaaca gcaagtgcta gaaaaagaac ttttagaagc tatctcttcc ctgcaaacaa
     1321 cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat gagtaatgat
     1381 atacaaccag aaattcgttt cccaggattt acggaagctt gggaacagcg taagtttgaa
     1441 tgcttacttg ataaaaaaga tggagtaagg aggggaccat ttgggagtgc cttaaagaaa
     1501 gaattctttg tatctaatag caattttgtt gtctatgaac aacaaaatgc tatttatgat
     1561 aattacgaaa caagatataa aattactgaa aaaaaatata atgaattaat ca
//
LOCUS       DADVXN010000219         1572 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.226, whole genome shotgun sequence.
ACCESSION   DADVXN010000219 DADVXN010000000
VERSION     DADVXN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.226"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(95..1225)
                     /locus_tag="KB942_002882"
     CDS             complement(95..1225)
                     /locus_tag="KB942_002882"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBB6853241.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1285..1512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          456 a          313 c          250 g          553 t
ORIGIN      
        1 aaataatata aaaggtaaat aaaaaagagt ggatttctcc actccatgaa aagccgactt
       61 tttgggacgc ttttttttgc caagcctcac atttttatag ccaaacttct tttgcgactt
      121 gctgcaataa ttggattttc ttccattgtt cttcttctgt taaatggttt ccttcttctg
      181 ttgaagcaaa cccacattga ggactgatac ttagattttc caatggtaca aattgttgtg
      241 cttcttttat tcgttctttt attttctccg tatcttccaa atctggaaac ttagaagtaa
      301 ctaatcctaa aacaacttta ctttctttgt tactccaatg tcttaaagga gtaaaatctc
      361 ctgctcggtc agaatcgtat tccaaaaaga aaccatcata atttgttgaa aacaactctt
      421 ctgcaatggg ttcatatcca ccggaaaaca gccaagaaga agcgtgattc ccgcggcaga
      481 tatgtgttgt gatcgtcaaa tcttctggtt tgtcttccag tgcgttattg ataatctctg
      541 tacaaatatg ggccatttct ttcggatcaa ttcctttttg aagaagtgct tctcttttag
      601 tttgatcagc tagaaacgcc caatttgtat catcgaattg caggtaacgg caaccttttt
      661 cataaaaagc atgaatcgct gctttgtaag tagaggacaa gtcctcataa aaatcagata
      721 gatcaggata aatctccttg atttttgaag aaccatcatt agataggatc tcttgtcgta
      781 aaatcatcgt gggactaggg atcgttgctt ttgcaacaaa agtatcatct gtttccacta
      841 cttctttcag aaaagcaaaa tcttttaaaa atgggtgatt tggattgaaa gataatttat
      901 ctactacccg aatattataa gctggtgctg cttttccgtg gaatgtttga ttatatccag
      961 tttctggaac atagccttcg ataccattta gttcttctaa aaaatcaaga tgccaaaatc
     1021 cacgccgaaa ttcaccatct gtaattcctt tgatgccgac tgctttttgg tcttccacta
     1081 atttttttat ttcctggtct tcgattgcac gaagttcttc ttttgaaagt aagccttctt
     1141 gaactttttt tcttgcttct ttgatcgatt gcgggcgtaa gaaacttccg acatgatcta
     1201 ctgtaaatgg tctatttttg ctcatttcac tcactctttt ctttgttttt tgtaaaaaaa
     1261 aatcctttta ctagcaaata ctagtaaaag gacgattata ctcgtggtac cacctttttt
     1321 cagaaaaaac attctttctc tcagcctgac aataaaatca gtggtcttgg taacgaaaga
     1381 ctgttcgttt ttgcctacct ttcgacaaaa aatcctctaa ggccattttc gacagtcaac
     1441 ttctagcttt ttacaccaac caaagcctct cttgaaccat tttctgtcta ctttccttct
     1501 catcggatac tattagaata aaattagaat agcatgtttg aaagaaaagt caatagggaa
     1561 taagaacaaa ca
//
LOCUS       DADVXN010000220         1538 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.227, whole genome shotgun sequence.
ACCESSION   DADVXN010000220 DADVXN010000000
VERSION     DADVXN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.227"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            22..1488
                     /locus_tag="KB942_002883"
     CDS             22..1488
                     /locus_tag="KB942_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBB6853242.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          326 g          501 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ctgttttt
//
LOCUS       DADVXN010000221         1480 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.228, whole genome shotgun sequence.
ACCESSION   DADVXN010000221 DADVXN010000000
VERSION     DADVXN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.228"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            64..972
                     /locus_tag="KB942_002884"
     CDS             64..972
                     /locus_tag="KB942_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6853243.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="KB942_002885"
     CDS             972..>1480
                     /locus_tag="KB942_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853244.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DADVXN010000222         1479 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.229, whole genome shotgun sequence.
ACCESSION   DADVXN010000222 DADVXN010000000
VERSION     DADVXN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.229"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            131..505
                     /locus_tag="KB942_002886"
     CDS             131..505
                     /locus_tag="KB942_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6853245.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="KB942_002887"
     CDS             complement(619..1248)
                     /locus_tag="KB942_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6853246.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="KB942_002888"
     tRNA            complement(1382..1454)
                     /locus_tag="KB942_002888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          500 a          225 c          260 g          494 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataatagga
//
LOCUS       DADVXN010000223         1477 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.230, whole genome shotgun sequence.
ACCESSION   DADVXN010000223 DADVXN010000000
VERSION     DADVXN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.230"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            92..502
                     /gene="spx"
                     /locus_tag="KB942_002889"
     CDS             92..502
                     /gene="spx"
                     /locus_tag="KB942_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6853247.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            516..872
                     /locus_tag="KB942_002890"
     CDS             516..872
                     /locus_tag="KB942_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6853248.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          541 a          210 c          272 g          454 t
ORIGIN      
        1 ttttgatttt ttttatattt tattagctaa taattggtat tttaaaagaa attactgtag
       61 aattggatta taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg
      121 tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa
      181 gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga
      241 aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga
      301 ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt
      361 gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat
      421 ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa
      481 caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag
      541 cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc
      601 aatttgttga cttagaagaa cgcgatttaa gtacagaaga agcagaagaa ttatgcagtt
      661 ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg
      721 cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca
      781 atctcctcag acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc
      841 agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga
      901 gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt
      961 ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta
     1021 ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt
     1081 ctgttgtttt aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt
     1141 tgacatgtta gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgtgaa
     1201 gcttgattct atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa tgataagtta
     1261 tgataaatga gttgctgtaa gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag
     1321 ttgacaatta cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat
     1381 cggggaacac tcctgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat
     1441 gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DADVXN010000224         1459 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.231, whole genome shotgun sequence.
ACCESSION   DADVXN010000224 DADVXN010000000
VERSION     DADVXN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.231"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..613)
                     /locus_tag="KB942_002891"
     CDS             complement(<1..613)
                     /locus_tag="KB942_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853249.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1459
                     /locus_tag="KB942_002892"
     CDS             901..>1459
                     /locus_tag="KB942_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6853250.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDR"
BASE COUNT          505 a          213 c          279 g          462 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatacgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgag
//
LOCUS       DADVXN010000225         1428 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.232, whole genome shotgun sequence.
ACCESSION   DADVXN010000225 DADVXN010000000
VERSION     DADVXN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.232"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            142..>1428
                     /locus_tag="KB942_002893"
     CDS             142..>1428
                     /locus_tag="KB942_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBB6853251.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKN"
BASE COUNT          502 a          189 c          292 g          445 t
ORIGIN      
        1 gggaattatt tggttacgag tcgaggggtt tttgaactaa tgtaaatcat ttgttagaat
       61 tgaccagaaa tttctatcgt acttttactt ttctatttga tatactgaaa tagaaaatat
      121 ttgttgatag gggtgttact aatggttggc aataagtggt acaaatgtga tcttcatatt
      181 cattcaactg cgagtcagtg ctttcaggat aaaaaggtta ctgctaagga atgggtagaa
      241 gaatgtttaa acaaaggact cgattgtgtt gcattaactg atcacaatac aggggaaaat
      301 attgatgaat ataagaaagt ggctatcgaa aatggcttag atttttttcc tggagtggaa
      361 ttaacgtgtg gagaaaatgg aactcattta ctagtcctat ttgataaaag tgcatgtaca
      421 aatgatatct ctgatttctt aaccaagatg ggtataaaaa gagaggattg ggccagcgct
      481 gcagcttgct caatgaaatc agttttagaa gttattgatt ttgctgagca cgagggcttg
      541 ctcgttattc ctgcacatat agatgagttt aatggaattt cttcagcaaa atttggaaat
      601 ctaggaaaga tatttgaatc agaaaatata tatgcagtac agacggttca aaaagaattt
      661 tttgaaaatc gctcacaaat gattccctca tctaagcgtg acactatcta tgatagtgtg
      721 aatgccagat atgatggtag agttggaaaa gatactttgg aatcttggta taaatcagtt
      781 gttgaggcag aaaaaaataa tatgacattt cttagctttt ctgataaccc acactcgaaa
      841 ggaaattcaa aacatggact ttggggaata ggcacaagat attcctatat aaaaatgaag
      901 gacgaacctg acttgggatc acttcgcgat gctttaatgc taggcgaaac tcgagttcat
      961 tctgattttt ctaatttctc cattaatgaa aatgaagttt tattggaaaa attaagcttt
     1021 tctgggacca ctcttagcac aaaagaagtt gtagtcgaat tcagcgataa tttaacttca
     1081 attattggag gaagaggaac aggcaaatct tgtattacaa gattcttggt ctatgtcctt
     1141 ggaaaggaag cagaacttga tcaattttct gaaatacaaa gtgattatca gaactttgca
     1201 caaattgaac ataatggctc tggaatattt ttgaaggata caattgtgaa actgaatatt
     1261 ttctataagg ggacaaaata tcaaataata agaacacagg atagacattc tatttatgaa
     1321 tttacacaag agaatggtct ggttgaaaaa gaaacagaac ggttgagaat gatttccgat
     1381 aaggtgagta tatactctca aaaacaaatt tatgaaattt caaaaaat
//
LOCUS       DADVXN010000226         1421 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.233, whole genome shotgun sequence.
ACCESSION   DADVXN010000226 DADVXN010000000
VERSION     DADVXN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.233"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(41..1180)
                     /locus_tag="KB942_002894"
     CDS             complement(41..1180)
                     /locus_tag="KB942_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBB6853252.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          364 a          305 c          260 g          492 t
ORIGIN      
        1 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
       61 taattccttg atatttttcg atcatatcca aataattatc gatttcaaca tactcatcta
      121 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
      181 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
      241 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
      301 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
      361 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
      421 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
      481 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
      541 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
      601 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
      661 gcgtatacat caaactgtaa ttggttggct ctccgatgat caaggcatct aagtcatcta
      721 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
      781 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
      841 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
      901 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
      961 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
     1021 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
     1081 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
     1141 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
     1201 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
     1261 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
     1321 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
     1381 gctagttatt gttagctttc ctttttcaag gatgatcttt t
//
LOCUS       DADVXN010000227         1398 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.234, whole genome shotgun sequence.
ACCESSION   DADVXN010000227 DADVXN010000000
VERSION     DADVXN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.234"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..705
                     /locus_tag="KB942_002895"
     CDS             <1..705
                     /locus_tag="KB942_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6853253.1"
                     /translation="DIKKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHP
                     TSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLE
                     NMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKI
                     IAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSV
                     ELREKVLRSIIDKSIE"
BASE COUNT          483 a          205 c          220 g          490 t
ORIGIN      
        1 gatattaaga aaaacacaaa agttggcgat gctactatta ttcgcttttg tcaaaaaatt
       61 ggtttttctg gtttttctga tttaaaaatt gaaattgcaa aggaagactt tagtcaaaaa
      121 aaagaacatc cgactagtgt aaagtactat gatgaaattg ctaatagttt aacagaagct
      181 ctctattcca ctattaggct tttagacgaa gaaaaattga atgaagccat taaaatgatc
      241 actcaatcaa aaaacatata tatatttgga gtgggatcta gtggaaatac tagtcttgat
      301 ttagaaaata tgtttcttcg cgtaggagtt caagcaaaag ctgtactaga ccctcatttt
      361 caagcccaag tcgcttcttt attaacagtt aatgatttag taatcatttt ttctttatct
      421 gggaaaacga aagatactta tgattcattg aaaatagcta aaaaaaatgg cgcaaaaatt
      481 attgctataa ctaactatat tcattcacct ataggaaagt ctgcagactt agttttacaa
      541 acagccattg aagaatttct taatggtgga tctttagctg gaaaaatatc acaactttat
      601 atttgtgatc tattagttca tggttatgaa caaaacaata atattgattc agttgaatta
      661 agagaaaaag ttttgcgctc tattattgat aaaagtattg agtgaactac ccccacttag
      721 ctaaacctaa cggtttttaa cgattgaagt aggcaattct tgggatgaga gcatacttga
      781 tagcttattt tttactaata gcggtggctc ttattgacca aatatccctg tagttcctac
      841 ggttcttgat tttatctaaa gatattttta tcgtggaagc tattgatcaa cttgaaagaa
      901 aaacgtgtag ctcattatca ctagtttatc gagtatggat gaagcaatgg atcatcctct
      961 cttaaataaa tttattaagg atctgatcct tcaaattttt tccatgatcg atgagcagga
     1021 acctactgaa tctaaacgac atcgggcaca aggaattatt aagattgcta agcgaatgtt
     1081 ttctataaag ggtgttctaa gctatacagt gttaattcaa aagattccca atcatcttgt
     1141 ttataagaac aacgttgaag attaaaaaag tgtttcaatg ctaaaattgc aaaatacttc
     1201 aatgttatga gacaaactgt ctataagaat caatttccac ccatttcatc acttgttaaa
     1261 agtttatgat ccttttgatt tttatattaa tttatctgta tttaatacag attttattta
     1321 ttagttcatc tgcctattgt attttatttt gttgccttac catctgaagt aaatatactc
     1381 ttaattgtca aattaagt
//
LOCUS       DADVXN010000228         1364 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.236, whole genome shotgun sequence.
ACCESSION   DADVXN010000228 DADVXN010000000
VERSION     DADVXN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.236"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            82..>1364
                     /locus_tag="KB942_002896"
     CDS             82..>1364
                     /locus_tag="KB942_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBB6853254.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DADVXN010000229         1364 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.237, whole genome shotgun sequence.
ACCESSION   DADVXN010000229 DADVXN010000000
VERSION     DADVXN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.237"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(140..1288)
                     /locus_tag="KB942_002897"
     CDS             complement(140..1288)
                     /locus_tag="KB942_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853255.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DADVXN010000230         1355 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.238, whole genome shotgun sequence.
ACCESSION   DADVXN010000230 DADVXN010000000
VERSION     DADVXN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.238"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..1355
                     /locus_tag="KB942_002898"
     rRNA            <1..1355
                     /locus_tag="KB942_002898"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DADVXN010000231         1346 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.239, whole genome shotgun sequence.
ACCESSION   DADVXN010000231 DADVXN010000000
VERSION     DADVXN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.239"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            33..>1346
                     /locus_tag="KB942_002899"
     CDS             join(33..474,474..>1346)
                     /locus_tag="KB942_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBB6853256.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPV"
BASE COUNT          564 a          173 c          235 g          374 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtag
//
LOCUS       DADVXN010000232         1318 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.240, whole genome shotgun sequence.
ACCESSION   DADVXN010000232 DADVXN010000000
VERSION     DADVXN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.240"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(290..439)
                     /locus_tag="KB942_002900"
     CDS             complement(290..439)
                     /locus_tag="KB942_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853257.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            901..1113
                     /locus_tag="KB942_002901"
     CDS             901..1113
                     /locus_tag="KB942_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853258.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          491 a          194 c          216 g          417 t
ORIGIN      
        1 ttacgtaagt attgatgatc tgtctaactt aatttgacaa ttaagattat attaaatatt
       61 atacagataa taacggaata ttatcatcga aacagctaac aaaattgtca taaaaaacga
      121 gatggttgat attagaactt gtgtttttgt ttaggtctag agataaagag agacaatttc
      181 caaatatatg taatgaatac aaaaagacgt ttgtcgcgga ttaccgttac aaacgtcttt
      241 tattgatttt ccgagatctc atcagtcgcc atttggaatg gttcactcct tatctagatt
      301 cgtttaatgc tcggactgct tgttcgattg taataccaaa cgcaacgtta ttttcgtttt
      361 gtgcatcaat cgccataaaa cggttagaag ttgtatcaaa agctacgcgg tattgcatat
      421 ttgtttttgt aaacgtcata aaattcatct cctttgtaat gtaatttagt tcttgaaagt
      481 ttcctttaaa ctttcgaact tataccaagt ttagcatcgt acgttttttg atgcaattaa
      541 aaagcattta tcattttcct tatttatttt ttcttaaatg gtttagctat tttcttttaa
      601 tccgataagt agcccaatca tcaaggttac aaatactatt gcttgaatat aaatcatatg
      661 ctcacctctt tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa
      721 tataaacaaa cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa
      781 gcagaaagat actcaaaata aaaaataaaa aaatggataa aaacgttttc aaatacaagg
      841 taatttgtta ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa
      901 atggtaaaag cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa
      961 ctaatcggct gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat
     1021 gaccaacgag actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt
     1081 gatattagcg cacctgctga attggtttct taaacagtat ttttgagaat tcgcaagaac
     1141 tcccttataa tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt
     1201 cagggaaaat gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg
     1261 ttcgtgcgat gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaaaa
//
LOCUS       DADVXN010000233         1317 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.241, whole genome shotgun sequence.
ACCESSION   DADVXN010000233 DADVXN010000000
VERSION     DADVXN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.241"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            25..1293
                     /locus_tag="KB942_002902"
     CDS             25..1293
                     /locus_tag="KB942_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBB6853259.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT          324 a          236 c          318 g          439 t
ORIGIN      
        1 ttttttattg aggaggaatt ttttatggaa aaacaattac cgagtccagt acgcatttta
       61 gtgtacatgc tgaagtggct ggtgatcaca gcgattctcg gtatattcat ggggagctta
      121 tctgcttttt tcttaaatag cctgacagtt gtgacagata tccgactatc acatccttgg
      181 ctcctttatc tactaccagt tagtggtgcg ctttttgctt atctctatgc gtatcacggc
      241 ggtctttcta gtcgtggaaa caacttagtg atcgatcaag gaaatggtgg agaagaaaaa
      301 atcccgttgc gacttattcc acttacactg tttggaacca taactaccca tttatttgga
      361 ggatctgttg gccgtgaagg aacagcagtc caaatggggg gagcgttagc agataataca
      421 gcacgtttgt tccgattgga taaatcagaa cgggaaatct tgattatcag tggaatcagt
      481 gcgggtttta gttctgtatt tggcacacct ttagctggaa cattatttgg attagaagtg
      541 ttggcaatcg ggaaagtccg aacagaagcg atcttcccta gcttctttgc tgcactgttt
      601 gcaaatttta tgacagaaag ctatggtgtt tctcatctac attaccaaat gggtgtgatt
      661 cctgaatgga gtatgttgct gttcttcaaa gtttttgttg ctgggattgc ttttggtttg
      721 attggttggg tattcagccg ttcgatcgtc tttttaaaag cgcgatatgc acaatggatt
      781 cctcatcctg tttggcgaaa tacgataggt ggggctgtag ttgtagccgc agcattgatt
      841 ttgcaaacac aacgttatct cggattgagt cttccattac tgcaggaatc atttaatgga
      901 caggcacatg tctatgattt tattgggaag ttattcttca cagttttgtc tttaggcgcg
      961 ggatatcaag ggggagaagt gacaccattg tttgaaattg gtgcaacgct tggtgcagca
     1021 ctagcgcctt tgctccatgt ttctgttcct tttcttgcag gattgggctt tatcggagta
     1081 ttttctggtg cgacaaatac accgattgcc tgctttgtga tggggatcga attgttcggt
     1141 tcacaagcgg cagtatactt ctttatgatc tgcttgatca gctttatgtg ttcaggcaac
     1201 agcggtattt attctgctca gcaagtagcg gtcagaaaag ggatactctt tttgccaata
     1261 gttaagagaa tgaaagaaaa aaacaaggtg tgacatatgt tttgtcacac cttgttt
//
LOCUS       DADVXN010000234         1311 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.242, whole genome shotgun sequence.
ACCESSION   DADVXN010000234 DADVXN010000000
VERSION     DADVXN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.242"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            102..>1311
                     /locus_tag="KB942_002903"
     CDS             102..>1311
                     /locus_tag="KB942_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6853260.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          412 a          258 c          252 g          389 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta a
//
LOCUS       DADVXN010000235         1300 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.243, whole genome shotgun sequence.
ACCESSION   DADVXN010000235 DADVXN010000000
VERSION     DADVXN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.243"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(62..280)
                     /locus_tag="KB942_002904"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="KB942_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="KB942_002905"
     CDS             complement(546..740)
                     /locus_tag="KB942_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853261.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="KB942_002906"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="KB942_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          461 a          217 c          177 g          445 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt
//
LOCUS       DADVXN010000236         1265 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.244, whole genome shotgun sequence.
ACCESSION   DADVXN010000236 DADVXN010000000
VERSION     DADVXN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.244"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            168..1208
                     /locus_tag="KB942_002907"
     CDS             168..1208
                     /locus_tag="KB942_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6853262.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          509 a          180 c          208 g          368 t
ORIGIN      
        1 cttaatgact ttaaaggtag tacataattc attttatttc tgattttagg tagtatttaa
       61 ttaaaaaaca gttttaatgc gtttgcattt tttatttatc tttgctattt tcgaaaagag
      121 taggtaattt tacataaata aaacaacgtg aaagtagagg atctacaatg actgatttta
      181 aattttttaa ggcggataga gtttacaacg aattatttta tcaatttcca aaagtcttta
      241 ttgtttctga cgaatacaaa aaaatgaaag attcaactaa gattgcctat atgcttttaa
      301 aagcaagatt agagatcgca atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt
      361 ttacttatac gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa aaagcgatag
      421 caatcaaaaa agagttggaa tcctttggtt tattattaca aaagcagatg ggatttaaca
      481 aacagttagg gaaaaataat cctaatagac tatatctagc agaattaaaa gtctcagaaa
      541 atgatatcta cttactcgaa aaatttgata gagagaatag ggaaaacgtt gataaatcag
      601 agggtatgaa aatcataccc accctcgacg aaaaatcaga cgctgaatcc cttggggctc
      661 aagagggtat gaaaatcata ccgtgccaaa acgttgataa atcagagggt atgaaaatca
      721 taccagaact taataataat atattagaca ctaatagaca caatatagac actgaaaaag
      781 accgcctaca agatcaattg ttgttagaca attttgagac aattatgaca aacgacagca
      841 ttgctacgtt tgtccctgaa cgagtattaa atttgataaa aacattttct tcaagttaca
      901 gtgaagctca aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct gaaatagaaa
      961 gtgatatttc gatagttttt gaagaactcg atagttatta tgtcaatgca gaacaagaat
     1021 tatacacgac actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc
     1081 agaacctgat ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag
     1141 tatcaagtga aaaacgtttg aattatgcaa gctccccaag cactattacg aaagactggt
     1201 tagagtgaaa ctttactgac aaagtgattg ttgcttcatg gagtaacatt aaaattaaaa
     1261 atttt
//
LOCUS       DADVXN010000237         1233 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.245, whole genome shotgun sequence.
ACCESSION   DADVXN010000237 DADVXN010000000
VERSION     DADVXN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.245"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..1101
                     /locus_tag="KB942_002908"
     CDS             <1..1101
                     /locus_tag="KB942_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6853263.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT          403 a          249 c          231 g          350 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gta
//
LOCUS       DADVXN010000238         1220 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.246, whole genome shotgun sequence.
ACCESSION   DADVXN010000238 DADVXN010000000
VERSION     DADVXN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.246"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..461
                     /locus_tag="KB942_002909"
     CDS             <1..461
                     /locus_tag="KB942_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBB6853264.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="KB942_002910"
     CDS             470..697
                     /locus_tag="KB942_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853265.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          377 a          196 c          204 g          443 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
      781 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
      841 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
      901 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
      961 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1021 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     1081 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     1141 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     1201 acattatttt gataataatg
//
LOCUS       DADVXN010000239         1211 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.247, whole genome shotgun sequence.
ACCESSION   DADVXN010000239 DADVXN010000000
VERSION     DADVXN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.247"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            67..1023
                     /locus_tag="KB942_002911"
     CDS             67..1023
                     /locus_tag="KB942_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853266.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1077..>1211)
                     /locus_tag="KB942_002912"
     CDS             complement(1077..>1211)
                     /locus_tag="KB942_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6853267.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          206 g          399 t
ORIGIN      
        1 acagggaatc ctaaaaacat tcggcttttt ttatataatc tttttaactc aacagagata
       61 agttttgtgg aattgtatac gctacctata attataaaaa cagaaaaaat gttaaaagat
      121 ttaaaacagt attatcctct gtctgcttat gaaaaaaaga aattattcca ctttttagca
      181 atcaacctgt tggctgaaca agctgattct ttagatattg ctagcaatca gtcgctattt
      241 ttttcggata taggatttta ccacttaaaa ggagtattag gagaaaaatc cgttattttt
      301 atcaataaga cactctcttg gctttatctt catgataaat tggatcaaaa atacttgaga
      361 aaaaatgaag atgaaacaat cgaatggttg aatgaacgat tcattgaaag ttttgaaaat
      421 cagtttaatc tgttgcgaga agagacgagc caattgatcc gaagagagtt aaccaaaata
      481 cattttaaac ttttgcatta cactattaat aaagctgatg aatttccact tgatgtttct
      541 ttttttagag aaaactatcc agcattttat tgctatttga tagagtatat aaatgatcta
      601 acgtcaaaac gcaaagagtt gcttcaagat aaattttttc tttttttcag ttatttatta
      661 ttattgatca atcacatacc cattcaactg atttctgaac cagtaaagat catcatcgat
      721 ttttcttatg gtgctgccta taatcaattt atcaagaaaa atttaagctt gtacattaat
      781 ctgaacacgg aagtgataga ggcaggaaag cctgagttcc cggatgttat aatcacgaat
      841 aggaataatc tatatgaaga aagtgcatct caagtagtgg tttggctcga tccgccaaga
      901 gcagtagact gggggaatct tacaaagatt ttgttgacta tccaagaaga gaaatacaaa
      961 gacagcaaaa ttgacaagca aatagacgat tttcctgaag aaatcatcga caggatcgaa
     1021 tagtgaactt cttttagaaa gtccgactgc tttttctaaa aatattattt gtcaaattaa
     1081 gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt
     1141 agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg
     1201 acttggttga a
//
LOCUS       DADVXN010000240         1153 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.248, whole genome shotgun sequence.
ACCESSION   DADVXN010000240 DADVXN010000000
VERSION     DADVXN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.248"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            38..910
                     /locus_tag="KB942_002913"
     CDS             38..910
                     /locus_tag="KB942_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6853268.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          334 a          220 c          280 g          319 t
ORIGIN      
        1 ttagtgattt cattctaaaa taaagggaga aatgtctatg ctatacggtg caattgaagc
       61 aggcggcaca aaatttgtgt gtgccattgg cgatgaagaa atgacgatca aggaaagagt
      121 gagctttccg acaacgacac ctgaggaaac aatgccttta gtcattgact tcttcaaaca
      181 ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg atatccatcg
      241 cgattccgcc acttatgggt acattacctc gaccccgaaa ttggcatggc aaaattttga
      301 tttcatcggt accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa
      361 tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta
      421 ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg
      481 gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc
      541 tggcagctgt ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga
      601 aaaccgctta gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga
      661 agcctcctat attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat
      721 catttttggc ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt
      781 tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc
      841 agcattagaa gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt
      901 acattcataa acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg
      961 aaaatttagt cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaaga
     1021 tcaagccttg aatgagataa attcttgctc gaaagttatg gaaatttcga tagccgtagg
     1081 ccacttgtat tgtgcaatag ttttttacaa aaaaaagggg gtgcggatat ttttttggac
     1141 ctttttaaca aat
//
LOCUS       DADVXN010000241         1150 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.249, whole genome shotgun sequence.
ACCESSION   DADVXN010000241 DADVXN010000000
VERSION     DADVXN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.249"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            110..958
                     /locus_tag="KB942_002914"
     CDS             110..958
                     /locus_tag="KB942_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6853269.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          393 a          202 c          195 g          360 t
ORIGIN      
        1 agattttaca aaaatatttt ttgtttgatt ttttcctact ctccttgtat ctttaacatt
       61 aatgagaacg gaaaaaatga taatcttatt atgaaaatga ggaattctca tgagcgtctc
      121 cataagacta caaatcaaaa caatgtacaa cgacttcagc ccaaaagaac aggctatcgc
      181 cgattatatt ttagaaaatc caagcaaagt aagtcatagt cctattagca atttagcaag
      241 tgaactgggc attgctgact ctactttttt tcaatttaca aaaaaacttg gttataatgg
      301 atttaaagat tttaaaatgg ccatgttgat ccaagaaaat gatctatctg ctatttctat
      361 ccacgaaaat attcagaaaa cagatactga gctcactatg gcgcaaaagg tgttcgagtc
      421 tagtatttca actttaaatg acacaaaaaa gttgttacaa caaaaggatc taaaaaaagc
      481 ggctactatt atcaatgaat ctaaacgtgt ctattttttc ggtgttggcg gttcagagat
      541 tgtagcaact gacgcttatc ataaattttt gcgttcccct atcgcaactt ctcacagcac
      601 ggattaccat attcaattga tggaagcttc tttattgacg gaaaaagact gtgctgtttt
      661 gatttctcac acagggcaat caaaagaaac aatccatatt gctgaaaccg taagaaaaac
      721 tggagcaaaa acaatcgtta ttactagcca agctaattct tcccttgcta aacttggcga
      781 tgtcgttttt atttctattt ctgaagaaac agaattccga tctgaagctt tagcttcccg
      841 tatttctcaa ctaagcattt tggactctct atatgtgatt ttgatgtttt acaacgaaaa
      901 agaatcacgt cgttctatcg ctaaagttcg acatactatt tctgaaatca aagaatagca
      961 ccataacaga aaaagcgaaa aatccataaa acaaaaaaag ccactaccaa atatttggta
     1021 gtggcttttt tggttctgca agggtggtac agaacatcaa tagtcggtgg gttagaacgt
     1081 atgtcatcgc atctttggag aaggttgtat tggtattatt cgagttactt gttagatgcg
     1141 ataacaaagt
//
LOCUS       DADVXN010000242         1143 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.250, whole genome shotgun sequence.
ACCESSION   DADVXN010000242 DADVXN010000000
VERSION     DADVXN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.250"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            54..260
                     /locus_tag="KB942_002915"
     CDS             54..260
                     /locus_tag="KB942_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853270.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(622..861)
                     /locus_tag="KB942_002916"
     CDS             complement(622..861)
                     /locus_tag="KB942_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853271.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          400 a          163 c          212 g          368 t
ORIGIN      
        1 tgaataacga aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac
       61 aaaaagtggt tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg
      121 aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat
      181 atactcacgt ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg
      241 aacatacttt agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc
      301 ttttgaaggc actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag
      361 gttcgcctag agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta
      421 tactgccaga actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg
      481 atccaatcat ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat
      541 cgtaaagcat ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat
      601 ttgctgaaga agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga
      661 cagtttcttt accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg
      721 agaatttttc tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga
      781 aggtatgttc tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact
      841 gataaacaga attcttttca ttatgtacac actccttata gtatcgttta gcatacacct
      901 atgctcagta ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt
      961 cttagtaata catatatgga gagggtgata tcccagttca aaccagatac attggttacc
     1021 atctgtatta acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa
     1081 cgtaggtatg atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga
     1141 tag
//
LOCUS       DADVXN010000243         1121 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.251, whole genome shotgun sequence.
ACCESSION   DADVXN010000243 DADVXN010000000
VERSION     DADVXN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.251"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            287..694
                     /locus_tag="KB942_002917"
     CDS             287..694
                     /locus_tag="KB942_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853272.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="KB942_002918"
     CDS             708..1109
                     /locus_tag="KB942_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853273.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          416 a          139 c          196 g          370 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact t
//
LOCUS       DADVXN010000244         1112 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.252, whole genome shotgun sequence.
ACCESSION   DADVXN010000244 DADVXN010000000
VERSION     DADVXN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.252"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(267..971)
                     /locus_tag="KB942_002919"
     CDS             complement(267..971)
                     /locus_tag="KB942_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853274.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          194 c          199 g          398 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DADVXN010000245         1095 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.253, whole genome shotgun sequence.
ACCESSION   DADVXN010000245 DADVXN010000000
VERSION     DADVXN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.253"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            266..>1095
                     /locus_tag="KB942_002920"
     CDS             266..>1095
                     /locus_tag="KB942_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6853275.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          386 a          152 c          186 g          371 t
ORIGIN      
        1 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
       61 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
      121 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
      181 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
      241 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      301 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      361 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      421 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      481 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      541 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      601 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
      661 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
      721 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
      781 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
      841 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
      901 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
      961 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     1021 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     1081 tttcaaatta gttta
//
LOCUS       DADVXN010000246         1076 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.254, whole genome shotgun sequence.
ACCESSION   DADVXN010000246 DADVXN010000000
VERSION     DADVXN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.254"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..863
                     /locus_tag="KB942_002921"
     CDS             <1..863
                     /locus_tag="KB942_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6853276.1"
                     /translation="EGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKI
                     KSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYS
                     GSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGM
                     VVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLL
                     KVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIK
                     KPKEILSKIS"
     gene            873..>1076
                     /locus_tag="KB942_002922"
     CDS             873..>1076
                     /locus_tag="KB942_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6853277.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNED"
BASE COUNT          434 a           99 c          181 g          362 t
ORIGIN      
        1 aagaaggtaa aaatatagaa gaaaatgata agaattcaat tattgttcat gaagaatttg
       61 ctaaacaaaa caatctaaaa ttaggtgatg aagttgatct tgaattacta gatattgaaa
      121 aaagtggaaa aataaaaagt cataaattta aaattatagg gatcttttct ggtaaaaaac
      181 aggaaacata tacaggatta tcttctgatt ttagtgaaaa tatggtgttt gtagattatt
      241 caacaagcca agagatatta aataattcag aaaataatga aattgcaaat aaaattttaa
      301 tgtattctgg tagtgcagaa tctacagact tagccttaaa caaattgaaa gagcttaaaa
      361 ttgatgagtc aaagtatttt gttgaaaaag attctaatgc gtttgaagag tctttagagt
      421 cagtgagtgg aataaaatat ataataaaag taatgactta ttctattatg ttaggtggga
      481 tggttgttct ttcattaatc ttgattctat ggttaaggga aagaatttat gaaataggta
      541 tattcttatc tattggaaca tctaagatac aaattataat gcaatttata ttcgagttaa
      601 tattcatatc gataccaagt ataatatctt ccttattttt aggaaatgta ttactaaaag
      661 taattgtaga tggattcatt aactcagagg attcaatgat ttccggtgga agtttaataa
      721 ataatagtag ttttatgtca aatataacaa cacttggaca aagttattta atattaataa
      781 gtattattgt tttatcagtt gtatttgctt cttcattaat gttaatcaag aagcctaaag
      841 aaatcttatc aaaaataagt taggagaaaa taatggatat attagaaata aagaatgtag
      901 catatagtta tgcaaattct aaagaaaaag ttttgtcagg agtaaatcaa aaatttgaac
      961 ttgggaagtt ttatgcgata gtaggaaagt caggaacagg aaaatctaca cttctttcct
     1021 tacttgctgg acttgataaa cctcaaacag gaaaaatatt gtttaagaat gaagat
//
LOCUS       DADVXN010000247         1069 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.255, whole genome shotgun sequence.
ACCESSION   DADVXN010000247 DADVXN010000000
VERSION     DADVXN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.255"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..969
                     /locus_tag="KB942_002923"
     CDS             <1..969
                     /locus_tag="KB942_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6853278.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DADVXN010000248         1044 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.256, whole genome shotgun sequence.
ACCESSION   DADVXN010000248 DADVXN010000000
VERSION     DADVXN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.256"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(17..>1044)
                     /locus_tag="KB942_002924"
     CDS             complement(17..>1044)
                     /locus_tag="KB942_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBB6853279.1"
                     /translation="IFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTS
                     PNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSF
                     LVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSID
                     RKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGET
                     DFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNI
                     FSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISY
                     LYLKKEN"
BASE COUNT          408 a          177 c          129 g          330 t
ORIGIN      
        1 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
       61 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      121 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      181 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      241 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      301 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      361 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      421 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      481 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      541 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      601 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      661 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      721 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
      781 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
      841 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
      901 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
      961 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1021 atattagcgc agctaaaaaa atta
//
LOCUS       DADVXN010000249         1035 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.257, whole genome shotgun sequence.
ACCESSION   DADVXN010000249 DADVXN010000000
VERSION     DADVXN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.257"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..>1035)
                     /locus_tag="KB942_002925"
     rRNA            complement(<1..>1035)
                     /locus_tag="KB942_002925"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DADVXN010000250         1026 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.258, whole genome shotgun sequence.
ACCESSION   DADVXN010000250 DADVXN010000000
VERSION     DADVXN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.258"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            98..838
                     /locus_tag="KB942_002926"
     CDS             98..838
                     /locus_tag="KB942_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853280.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          376 a          184 c          199 g          267 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
      961 ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg
     1021 tttgca
//
LOCUS       DADVXN010000251         1001 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.259, whole genome shotgun sequence.
ACCESSION   DADVXN010000251 DADVXN010000000
VERSION     DADVXN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.259"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            370..1001
                     /locus_tag="KB942_002927"
                     /pseudo
     CDS             370..1001
                     /locus_tag="KB942_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DADVXN010000252          996 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.260, whole genome shotgun sequence.
ACCESSION   DADVXN010000252 DADVXN010000000
VERSION     DADVXN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.260"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..505
                     /locus_tag="KB942_002928"
     CDS             <1..505
                     /locus_tag="KB942_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6853281.1"
                     /translation="SFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQNNSIM
                     HKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVGGTCE
                     DYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKTAYNM
                     LWRIYNR"
     gene            519..>996
                     /locus_tag="KB942_002929"
     CDS             519..>996
                     /locus_tag="KB942_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6853282.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKV"
BASE COUNT          400 a          103 c          184 g          309 t
ORIGIN      
        1 atcatttggt gataacaaaa taatttttgc agaagataat tggtttcaat tacaagtatt
       61 tatgacaaat acaagaatct gtttcgtaga taaattgtgt tattattatc gccaacaaaa
      121 taattctata atgcataagc caaaaagtaa tttggttttt agacatggaa atatgattgc
      181 tgattatgaa aagttgataa ggcaaaatgg aaacaacata atagataaaa aagcttgtat
      241 gttggttgca tttgatgtac taattatgga gatttttaat caaaaaaacg ttggtggaac
      301 atgtgaagat tataagaatg cattagtttc tttgaaacat cataaaatat tgagagaaaa
      361 tatttcaaat atagttagaa tgaaagcata tgaacttgaa cctaatatga gaaagaagtt
      421 agcccttgtg accattagta taatgtttaa ttttaaaatg tataaaacag cgtataatat
      481 gctctggaga atatataatc gataaagagg taggaaatat gaggaaattg acctttgtga
      541 taggaattat gggaaaagga ggggcagaac gagtaattgc gaatttggca aataatttta
      601 ttcaagataa ctgggaaatt gatattataa ctatatatgg gaatcggcaa gactatgaac
      661 tgaatagaaa aatacaggta catccaataa tatgtaaaag ttcgtttcgt gcatttagac
      721 ctttggaacg ctttaaggaa cttaggaaaa aaataaggga aatacagcct gatctggtta
      781 tttcgttcct tgctgatgta aatatccatg tcattatttc attgttagga gtaaaacaaa
      841 aattaattgt ttctgaacga aatgatccac aacggaatcc agaagaacca tggattagga
      901 aaattagaga ttttctttat aaaaatgtag atggaattgt ttttcaaaca ccagacgcaa
      961 aaaaatattt tagtaagatc aatgataaaa aagtaa
//
LOCUS       DADVXN010000253          987 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.261, whole genome shotgun sequence.
ACCESSION   DADVXN010000253 DADVXN010000000
VERSION     DADVXN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.261"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>987
                     /locus_tag="KB942_002930"
     CDS             <1..>987
                     /locus_tag="KB942_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6853283.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKMF"
BASE COUNT          375 a          159 c          189 g          264 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgttt
//
LOCUS       DADVXN010000254          972 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.262, whole genome shotgun sequence.
ACCESSION   DADVXN010000254 DADVXN010000000
VERSION     DADVXN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.262"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            100..>972
                     /locus_tag="KB942_002931"
     CDS             100..>972
                     /locus_tag="KB942_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6853284.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNN"
BASE COUNT          340 a          169 c          179 g          284 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgccgcatct
      961 aaacgaaata at
//
LOCUS       DADVXN010000255          943 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.263, whole genome shotgun sequence.
ACCESSION   DADVXN010000255 DADVXN010000000
VERSION     DADVXN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.263"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>943
                     /locus_tag="KB942_002932"
     rRNA            <1..>943
                     /locus_tag="KB942_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DADVXN010000256          917 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.264, whole genome shotgun sequence.
ACCESSION   DADVXN010000256 DADVXN010000000
VERSION     DADVXN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.264"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>917
                     /locus_tag="KB942_002933"
     CDS             <1..>917
                     /locus_tag="KB942_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6853285.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLP"
BASE COUNT          316 a          176 c          166 g          259 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcct
//
LOCUS       DADVXN010000257          909 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.265, whole genome shotgun sequence.
ACCESSION   DADVXN010000257 DADVXN010000000
VERSION     DADVXN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.265"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            6..>909
                     /gene="bsh"
                     /locus_tag="KB942_002934"
     CDS             6..>909
                     /gene="bsh"
                     /locus_tag="KB942_002934"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBB6853286.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNK"
BASE COUNT          312 a          122 c          166 g          309 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaag
//
LOCUS       DADVXN010000258          878 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.266, whole genome shotgun sequence.
ACCESSION   DADVXN010000258 DADVXN010000000
VERSION     DADVXN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.266"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            49..>878
                     /locus_tag="KB942_002935"
     CDS             49..>878
                     /locus_tag="KB942_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HBB6853287.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          305 a          130 c          160 g          283 t
ORIGIN      
        1 tgtagaatag agttaccaca acacattcac agaaaagagg acttccatat gaacgatttt
       61 actacagaaa ttatggaaat actaatcaat aaaggtgatt tggatgattt gtttcgtcgt
      121 cacctagaat tggctattaa cgctttatta caagctgaat taacggtatt tcttgactat
      181 gaaaagtatg atagagctgg atttaattca ggtaattccc gtaatgggaa ttattcgcgc
      241 tcttttaaaa cagagtacgg tgaattgaat ttagtaatac ctagagatag gaatggagac
      301 ttttctcaac aaactttacc tgcttataaa agaaccaatg actctttaga aaccactatt
      361 attcagctag ttcaaaaagg aattactatg tccgaaatct ctgatctaat cgaaaaaatg
      421 tatggtcatt attacaaacc acaaactatt tcaaatatga gtaaaattgt gtctgaagat
      481 gtttcggcat ttaaagaaag aactttagaa gctaaatact cagtcatttt tatggatgct
      541 actcatattc ccttaaaaag acaaaccgta tcaaaagaag ctgtctatat agtaattggt
      601 atcaggttgg atggaactaa agaggttcta ggatttacta tagctccaac tgaatctgct
      661 tatgtttgga aagagattct tcaagatcta aaagaccgtg gtttagaaga ggttttgtta
      721 gtcgtaacag atggtttaag tggtattgac gatagtattc atagtgttta tccaaatgct
      781 caatttcaac aatgttgtgt ccatatatct agaaatatcg ctcataaggt tcgtgttagt
      841 gatcgacaag aaatctgtag cgatttcaaa ttagttta
//
LOCUS       DADVXN010000259          858 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.267, whole genome shotgun sequence.
ACCESSION   DADVXN010000259 DADVXN010000000
VERSION     DADVXN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.267"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..117
                     /locus_tag="KB942_002936"
     CDS             <1..117
                     /locus_tag="KB942_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBB6853288.1"
                     /translation="KKILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            245..796
                     /gene="raiA"
                     /locus_tag="KB942_002937"
     CDS             245..796
                     /gene="raiA"
                     /locus_tag="KB942_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBB6853289.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          307 a          151 c          177 g          223 t
ORIGIN      
        1 aaaaaaatat tgattattga cgatgtctat acaacaggac agacgctttt tcatgcagca
       61 gattgcttgc agaacgcaca gccgaaaatc atccgaacca tttctttggc aagatgaatc
      121 agatgtggaa atttgatagc gatttaattt gcaaataacc tagagttcga tataatagaa
      181 ttaagaagag ggaaagagtg gtccttctcc ttcttgattg tggtagacga aaggggtaat
      241 ttttatgttt agatataatg tacgcggcga aaatatcgaa gtaactgaag cgattcgcga
      301 ctacgttgag aaaaaagtag gcaaactaga acgctatttc agtgacgcac ccgatgcaac
      361 ggcgcatgtg aacttaaaag tttatacaga aaaaacagca aaagtggaag taacgatccc
      421 attaccttat cttgtattac gtgccgaaga aacttctcca gatttatacg caagtatcga
      481 tttggttgtt gacaaattgg aacgccaaat ccgcaaattc aaaacaaaaa ttaatcgtaa
      541 atcccgtgaa acgggcatga atactgcaga tgctgctgct ttattcaatg aagaggacga
      601 aaacgaaaat gattctgaat tagatatcgt acgtacgaaa cgcctttcat tgaaaccaat
      661 ggatagtgaa gaagcagtct tacaaatgaa catgctggga cataacttct ttatttttga
      721 agatgcagaa acaaatggta caagtatcgt ttaccgccgt aaagatggaa aatacggact
      781 gattgaaaca gactgatcag ccttacttaa ataacaacga aaacttgcag cagctactga
      841 cagctgccgc aagttttt
//
LOCUS       DADVXN010000260          854 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.268, whole genome shotgun sequence.
ACCESSION   DADVXN010000260 DADVXN010000000
VERSION     DADVXN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.268"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(378..578)
                     /locus_tag="KB942_002938"
     CDS             complement(378..578)
                     /locus_tag="KB942_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6853290.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          308 a          132 c          135 g          279 t
ORIGIN      
        1 ttattttcgg aaaaagctaa tatagctaaa attgaaacat ccatctggaa tttgtctttt
       61 tcgttgcttt gctcataaaa atctgcatat tcatcactaa tattttttct aataaattct
      121 tccatcataa agatcacctc gaaaagagta tacgaacaaa cgttcttttt gtaaaggtgg
      181 aatttattat actaaataaa taggtgaaca agtacttgtg ccaaattatg tgccaaaaaa
      241 aatcgaattt aataaaacac aaacaaaaag aaatcctatt atgataacat ttcttataat
      301 aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa cccctgtatc
      361 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
      421 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
      481 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
      541 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
      601 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
      661 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      721 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      781 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
      841 tctatgcttt atat
//
LOCUS       DADVXN010000261          843 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.269, whole genome shotgun sequence.
ACCESSION   DADVXN010000261 DADVXN010000000
VERSION     DADVXN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.269"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..511
                     /locus_tag="KB942_002939"
     CDS             <1..511
                     /locus_tag="KB942_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HBB6853291.1"
                     /translation="EYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQV
                     EKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEI
                     DEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKN
                     LVKSYLKMN"
     gene            514..>843
                     /locus_tag="KB942_002940"
     CDS             514..>843
                     /locus_tag="KB942_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HBB6853292.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNS"
BASE COUNT          348 a           95 c          166 g          234 t
ORIGIN      
        1 agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa attgggatat
       61 tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa ttgaagattt
      121 aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg atgagtattc
      181 aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta aggaaattcc
      241 caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa aagttacaga
      301 cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc gggctaagat
      361 tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag agctgaataa
      421 atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag gtatcaaaaa
      481 cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga ttctttatat
      541 caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga aataaagctt
      601 aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc atatcaaaat
      661 ctctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac aggaatagag
      721 gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag agcttgtatt
      781 aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct gacaaaaaat
      841 tcc
//
LOCUS       DADVXN010000262          834 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.270, whole genome shotgun sequence.
ACCESSION   DADVXN010000262 DADVXN010000000
VERSION     DADVXN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.270"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            201..785
                     /locus_tag="KB942_002941"
     CDS             201..785
                     /locus_tag="KB942_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6853293.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          266 a          123 c          172 g          273 t
ORIGIN      
        1 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
       61 ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
      121 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
      181 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
      241 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
      301 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
      361 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
      421 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
      481 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
      541 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
      601 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
      661 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
      721 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
      781 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtga
//
LOCUS       DADVXN010000263          830 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.271, whole genome shotgun sequence.
ACCESSION   DADVXN010000263 DADVXN010000000
VERSION     DADVXN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.271"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            17..>830
                     /locus_tag="KB942_002942"
     CDS             17..>830
                     /locus_tag="KB942_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853294.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          263 a          116 c          120 g          331 t
ORIGIN      
        1 tccagcattt aaaaacatgg aaatatttgg cttttatgat acggcatttc acattaatcg
       61 tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt ttagatcata
      121 tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat ttttattgat
      181 caagtttact aatttagcaa taggctataa cctttttctt tatattgtta cgctcattac
      241 tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta ctagctcctt
      301 tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc ttcgaggggc
      361 aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt ttataaagct
      421 ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt tattaattta
      481 cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt taattattca
      541 attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa aagctgctat
      601 agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga cattgtatca
      661 agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc tgggtaaaga
      721 gtttttgatt ggaagtatag attcagaatt actatcattt ggtattggct taactttgct
      781 tatttgttta attatactaa catttaactt taaaaaatta ggaatcttat
//
LOCUS       DADVXN010000264          814 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.272, whole genome shotgun sequence.
ACCESSION   DADVXN010000264 DADVXN010000000
VERSION     DADVXN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.272"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            8..81
                     /locus_tag="KB942_002943"
     tRNA            8..81
                     /locus_tag="KB942_002943"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="KB942_002944"
     tRNA            121..210
                     /locus_tag="KB942_002944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="KB942_002945"
     tRNA            225..296
                     /locus_tag="KB942_002945"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="KB942_002946"
     tRNA            312..384
                     /locus_tag="KB942_002946"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="KB942_002947"
     tRNA            394..466
                     /locus_tag="KB942_002947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="KB942_002948"
     tRNA            502..577
                     /locus_tag="KB942_002948"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="KB942_002949"
     tRNA            579..659
                     /locus_tag="KB942_002949"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="KB942_002950"
     tRNA            666..739
                     /locus_tag="KB942_002950"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="KB942_002951"
     tRNA            742..814
                     /locus_tag="KB942_002951"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          187 c          224 g          225 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgcc
//
LOCUS       DADVXN010000265          810 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.273, whole genome shotgun sequence.
ACCESSION   DADVXN010000265 DADVXN010000000
VERSION     DADVXN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.273"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>810
                     /locus_tag="KB942_002952"
     CDS             <1..>810
                     /locus_tag="KB942_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853295.1"
                     /translation="IVVPKNKSEDGNYELEVLQFKIEQKSSERFMSNQLVKIKKWLPM
                     IDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYK
                     GDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSK
                     SNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERD
                     SYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWT"
BASE COUNT          329 a           83 c          148 g          250 t
ORIGIN      
        1 gatagttgtt cccaaaaata aatcagaaga tgggaattat gagttagaag ttttgcaatt
       61 taagattgaa caaaagtcca gtgaaagatt tatgagcaat caattagtga aaatcaaaaa
      121 atggctccct atgattgatc aattagtatc taataataag gaagctaaaa atttatgtat
      181 ttttgtctta ttattacatg gaagagttat taacttagtt aatcgaaaaa gtacgactaa
      241 atttgcaaac tcattctggt ttttaaaaaa tattaataaa acagtggaaa agaatggagt
      301 ttataaaggt gattttcatt ttcaaaatgc tgagactatt gaacgagaac atcattattt
      361 cgatttatta gtgaatacag tggtagaatt agcgaaatgg gcaaagtcaa acgaaactta
      421 tagtcaagta cttaaaagaa ttatgagtca agaaacttca tttatctatg agttaagcaa
      481 gtcaaatttg aaatatactt ataatgaaga tgaacttgaa attttagata atccagaaaa
      541 ttcacaatgg attaaagagt tgaagagaga agactttatt gagacactaa gaattctaaa
      601 atcgttattt gatttgaaat tagccaaagc taccatcatg ggaattgaaa gagattcata
      661 ttattgggag gaaattggac tatatgctcc ccagattgaa ggtgaagata aagagaaaag
      721 gatagacaac attattaact atattaatag tttaagtgaa cctttaaaat gtagaggact
      781 tgattttata ggaacattag gatggacaga
//
LOCUS       DADVXN010000266          796 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.274, whole genome shotgun sequence.
ACCESSION   DADVXN010000266 DADVXN010000000
VERSION     DADVXN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.274"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(372..445)
                     /locus_tag="KB942_002953"
     tRNA            complement(372..445)
                     /locus_tag="KB942_002953"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Ile,seq:cat)
BASE COUNT          278 a          132 c          129 g          257 t
ORIGIN      
        1 actaattaac taccactcca attataagtc tttttcccat tattttttac agttatatgg
       61 tatgcttttt aatggcttca aatataaaag agtttaaagc gtaacacact tatggggaag
      121 tggtttttgg ggaacgcttt aaactcttct ttattattat ctcacaatat aacccaaatg
      181 tctttccatt taaaaatcaa agtaaaactt ttcaaatata cagaagtata actatgtgaa
      241 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
      301 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt
      361 ggctacatta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      421 ctctgaccaa ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc
      481 agtagaatgc gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt
      541 gatagtgaag atatgcgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa
      601 acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa
      661 attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg
      721 tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg
      781 aggtgaactt ttatgc
//
LOCUS       DADVXN010000267          796 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.275, whole genome shotgun sequence.
ACCESSION   DADVXN010000267 DADVXN010000000
VERSION     DADVXN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.275"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(46..291)
                     /locus_tag="KB942_002954"
     CDS             complement(46..291)
                     /locus_tag="KB942_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853296.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(285..686)
                     /locus_tag="KB942_002955"
     CDS             complement(285..686)
                     /locus_tag="KB942_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6853297.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          255 a          138 c          111 g          292 t
ORIGIN      
        1 agaagattct tttaaacaga aaaagtggaa aggggatttt tttaattagt taattttaaa
       61 ttttaagatc gcttctatta atttaatttc tagctgactt atcatatatt catctacata
      121 ggtaacgaat tgacctacct cgttaaatcc atttgtcaaa cataacttag ccatataaga
      181 atgataatgt ttgagtatca tcttgatcga aattggatct ccttctagag cagataagat
      241 aatggatagt ggaactaata ggtactgccg ctttttagat ttatctacca taggtcatac
      301 cccattttca attgttcaac tgccttcttt cgttgatacc agataccact tgtgctcata
      361 ttgagaattt taccgatttc agcatcatta aaacctgcaa aataataaag taggataatc
      421 tttcttctta tctccgataa ctgatcaatt gctgctgata acttttgatc tgaaattaat
      481 atttccattc caaataagaa atagacatct tgttcatcaa aatgagtttg ggaatacctg
      541 ccttcatttg aatctgccaa tggttcctca ttatccttta tgttagcgtt tcgttttcga
      601 atagacttag cttcgtttcg taatactttt ttacagtagg catcaaattg gtgttgtata
      661 gatatgtcat tgtaatgttg ttgcatgata atatgctcct ttttccccct ttccactaat
      721 acaacaatca gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaatattta
      781 tcattcttta aacaga
//
LOCUS       DADVXN010000268          785 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.276, whole genome shotgun sequence.
ACCESSION   DADVXN010000268 DADVXN010000000
VERSION     DADVXN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.276"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            46..>785
                     /locus_tag="KB942_002956"
     CDS             46..>785
                     /locus_tag="KB942_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBB6853298.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          309 a          127 c          148 g          201 t
ORIGIN      
        1 agttgttaaa tttgtttacg aatataaaag aaaggaatga gaaatatgaa cacaccgcaa
       61 atttttaatt tcgaacaaaa cgaagttcgg acaattttag taaatgatga accatatttt
      121 gtaggcaaag acgttgcgag tgttttgggt tattcaaaca ctaaagatgc tttgtcgcgg
      181 catgtagatt tggaagataa gatggggtcg cgaatcacga cctcaggtca atcaagagag
      241 atgacaatca tcaacgaatc tggtttgtac agtttaatct taaaatcaaa acttccctct
      301 gccaaaaaat ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag aaaacatgga
      361 ggttatctaa ctccagaaaa agtagaagaa gctttgctta atccagatac aatcattcaa
      421 ttagcaactc aactaaaaga agaaagaact ggaagattaa tcgcagaaca aaagattgca
      481 gagtacgaac ctaaaatctc ctatttagat agcatattat cttctacaga ttcagtaaca
      541 attagtcaga ttgcagcaga ttatgggatg tctccacaac agatgaataa attacttcat
      601 aaactaggta ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa acacatgaac
      661 caaggataca caaaatctca tacaactgag atcccgaaag ccgatggtgg cactaaaatt
      721 gtaatgaata ccaaatggac acagaaaggg cgtctattta tctacgaatt actaaaaaaa
      781 gaagg
//
LOCUS       DADVXN010000269          776 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.277, whole genome shotgun sequence.
ACCESSION   DADVXN010000269 DADVXN010000000
VERSION     DADVXN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.277"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..446)
                     /locus_tag="KB942_002957"
     CDS             complement(<1..446)
                     /locus_tag="KB942_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853299.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          233 a          135 c           96 g          312 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca
      721 ataataaaaa tttactattt gttatcttaa ccttactttt ctttctcttc tttttt
//
LOCUS       DADVXN010000270          747 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.278, whole genome shotgun sequence.
ACCESSION   DADVXN010000270 DADVXN010000000
VERSION     DADVXN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.278"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..464)
                     /locus_tag="KB942_002958"
     CDS             complement(<1..464)
                     /locus_tag="KB942_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBB6853300.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATV"
BASE COUNT          228 a          151 c          110 g          258 t
ORIGIN      
        1 actgttgcca ttccaccaga cacttgctta aagcgaaaaa tttcttcaat gtccatgcct
       61 aattctttgg atattctctt gtctgaccat ccatttttta ctaatctttg gatgacaatt
      121 tccattcctc ttatttcatg ggtccctctt gcacgattgt gtcgaattgt tgaagccata
      181 cgttcttcta tgggtttatc aataactgtt accggtacaa cttttaagtt gagtctttcc
      241 ttagcaacac gatacctatg aagcacatca accaagatat acttctcttc tttttccttg
      301 taataacaaa ctaatggttg agtaaagcca tcttcaatga tagaaagctc caataagttc
      361 atttccggag acgctactcg attgggatta taatcgttcg cctgtatttt ggtaattgga
      421 acaagccgaa cgtccatcac agggaaagtt atgtctttat tcatacgctt gtatacccta
      481 ctttcttaaa aagtagggta tactcaagaa tccgattaat tatcttctca atttggataa
      541 tatatctcat cagtcttctt gtcactttat caacatgatt ttgctgcaaa atgggatacc
      601 gacagtactg gactggtaat caggataaaa gcaaaaataa ttaatttgta tcctaaaaaa
      661 tccataaaaa ttctctcaca ttttcgggta ttaattcgag aatcacacct ataaatttat
      721 tactatataa gggatactta aaccaaa
//
LOCUS       DADVXN010000271          718 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.279, whole genome shotgun sequence.
ACCESSION   DADVXN010000271 DADVXN010000000
VERSION     DADVXN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.279"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..187)
                     /locus_tag="KB942_002959"
     CDS             complement(<1..187)
                     /locus_tag="KB942_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBB6853301.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHF"
     gene            complement(205..525)
                     /locus_tag="KB942_002960"
     CDS             complement(205..525)
                     /locus_tag="KB942_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853302.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          247 a          134 c           94 g          243 t
ORIGIN      
        1 caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct tctgcagttt
       61 caccgtatag attttcattc aaaaaaatga ttctgggttt tggatagtag aaaccaggct
      121 ctttcatctc catataaact actttcaaat tgtattcgct caacatttct ttcaatttca
      181 aatacataca aaccatcact ccaactattc attttcctct aaagctttag caattgcaat
      241 cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtc
      301 ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac
      361 agagacatcg aaatagtcag caatccgact taattcatct gaattaggag tattattttt
      421 ccactttgct agataaccgt tagaatatcc aaaattaatt tctaactgcc gtatagatat
      481 ctttttcctc ttcgccaagt cttttattat ttcataggta ttcatcgata aaacaacctt
      541 tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac taattctgag
      601 taatgagcta tactatatct tgtaaacaga tttaacaact aaaaagacaa caaaaaacac
      661 tattgatatg taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt tatgtctt
//
LOCUS       DADVXN010000272          717 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.280, whole genome shotgun sequence.
ACCESSION   DADVXN010000272 DADVXN010000000
VERSION     DADVXN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.280"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(132..509)
                     /locus_tag="KB942_002961"
     CDS             complement(132..509)
                     /locus_tag="KB942_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853303.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          233 a          117 c          122 g          245 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaaca
//
LOCUS       DADVXN010000273          698 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.281, whole genome shotgun sequence.
ACCESSION   DADVXN010000273 DADVXN010000000
VERSION     DADVXN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.281"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            66..>698
                     /locus_tag="KB942_002962"
     CDS             66..>698
                     /locus_tag="KB942_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853304.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDIS"
BASE COUNT          289 a          106 c          103 g          200 t
ORIGIN      
        1 acttttaaat acttttattt tataaatatg atatactatc tttaaaaata aaaaggagaa
       61 aaaagatgaa gccaaatcct tcagaagcag gaaaacgaat caaacaatta cgcctttcat
      121 gtggttttac tatggaagaa cttggaagga aaatagataa ttcaccacgt gcgaccatat
      181 ccaactggga acgtgggacc aatttaccaa atccacaaaa gctgaaatta ttatcaacta
      241 tcactaattc aacaattgac tggattaaat ggggaacttt agaagaatat ataacttcat
      301 accttatcga tatagggtat gagctatata ttaaggactt cccagaaata cctcacaaag
      361 tctttaaaga cattcaagaa agatatagtg atacgttttc gctagataaa gactatgagt
      421 tattgaatcc tataataaaa aatatattta caaaatatta ctcaaaagat tttgaaattt
      481 atctaaatga gatacttgct aataatatca atcctctgat tgaagagtac gctaaccaaa
      541 gacactctat ctctaaagag ctatttttca aaagagccaa cagtaaactt caagacaaga
      601 taagaaatgg tgatttcaaa tacggtgaaa aagaaagaat gattgattct ttaaaacagc
      661 tattagacga aatggactta gcctataaag atatttca
//
LOCUS       DADVXN010000274          625 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.282, whole genome shotgun sequence.
ACCESSION   DADVXN010000274 DADVXN010000000
VERSION     DADVXN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.282"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            1..625
                     /locus_tag="KB942_002963"
                     /pseudo
     CDS             1..625
                     /locus_tag="KB942_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DADVXN010000275          598 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.283, whole genome shotgun sequence.
ACCESSION   DADVXN010000275 DADVXN010000000
VERSION     DADVXN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.283"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..171)
                     /locus_tag="KB942_002964"
     CDS             complement(<1..171)
                     /locus_tag="KB942_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853305.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKK"
     gene            complement(181..>598)
                     /locus_tag="KB942_002965"
     CDS             complement(181..>598)
                     /locus_tag="KB942_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853306.1"
                     /translation="NKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPL
                     DRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNW
                     KEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          179 a           99 c           89 g          231 t
ORIGIN      
        1 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
       61 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
      121 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
      181 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
      241 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
      301 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
      361 tatcgtttta gaagctgctc gagcaagtaa tggagaaact attaaataat tttcagtaac
      421 agttaagtcg ttagttgggt taccaatggc tattccgttc ctatctaaag gagttaattc
      481 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
      541 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattt
//
LOCUS       DADVXN010000276          519 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.284, whole genome shotgun sequence.
ACCESSION   DADVXN010000276 DADVXN010000000
VERSION     DADVXN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.284"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            114..413
                     /locus_tag="KB942_002966"
     CDS             114..413
                     /locus_tag="KB942_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853307.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
BASE COUNT          184 a           87 c          111 g          137 t
ORIGIN      
        1 gaattgtaga gggaggtaaa gtcgttggaa gataaaatca ttcttacaaa atcagagctt
       61 caagaaatgt taaacatcga gtatggaacg ataattttta ggagggataa taagtgaaaa
      121 aaccaacact ttcagagctg atagaagctg ctgagaaggc agtaaaacca gacgactggt
      181 accgacaaag tttaatcttg gagaagttcc acggcatgtc aaaaactact ttagttgaat
      241 actgcaagga aatggaaaca attcctgaat ttgcagaagg aatacttcgt ccaggtcact
      301 caaccacatt tattcattac catactttta tttggttttt aaagtggaaa gacgcaaaca
      361 aatatcgtgt aaaaggttta tcgccttcag atgttttgaa ggaggcaagc taatgggcaa
      421 attcaacaga gcgctagtat tcagcacacc attcatcatc tacgctttag gtctttgggg
      481 aagcagacaa gcattaatcg gcacaatcgt atatatggt
//
LOCUS       DADVXN010000277          516 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.285, whole genome shotgun sequence.
ACCESSION   DADVXN010000277 DADVXN010000000
VERSION     DADVXN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.285"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(107..>516)
                     /locus_tag="KB942_002967"
     CDS             complement(107..>516)
                     /locus_tag="KB942_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBB6853308.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DADVXN010000278          503 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.286, whole genome shotgun sequence.
ACCESSION   DADVXN010000278 DADVXN010000000
VERSION     DADVXN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.286"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            112..306
                     /locus_tag="KB942_002968"
     CDS             112..306
                     /locus_tag="KB942_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853309.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          213 a           65 c           83 g          142 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cat
//
LOCUS       DADVXN010000279          495 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.287, whole genome shotgun sequence.
ACCESSION   DADVXN010000279 DADVXN010000000
VERSION     DADVXN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.287"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
BASE COUNT          168 a           96 c           57 g          174 t
ORIGIN      
        1 aaaaataaga atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt
       61 attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca
      121 cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct
      181 tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat
      241 ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga
      301 ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc
      361 aagacatctc gattcatatt caagatagga atctttttat caacatcact ttattcgtat
      421 tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc
      481 cattcaacga ccaat
//
LOCUS       DADVXN010000280          486 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.288, whole genome shotgun sequence.
ACCESSION   DADVXN010000280 DADVXN010000000
VERSION     DADVXN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.288"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..125)
                     /locus_tag="KB942_002969"
     CDS             complement(<1..125)
                     /locus_tag="KB942_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB6853310.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="KB942_002970"
     CDS             complement(143..463)
                     /locus_tag="KB942_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6853311.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          138 a          104 c           61 g          183 t
ORIGIN      
        1 ccatatagat tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct
       61 ttcatttcca tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaac ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt
      241 ctgataaatc cacatgttta ggggtttctt tttcttcgcg ccccagaaga tagtctacag
      301 agacatcgaa atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa
      361 gtctatataa gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc
      421 cctgtttttt cgctaactct tttattctct caaacgttat catatcaata ttcctttcgt
      481 tcaaga
//
LOCUS       DADVXN010000281          468 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.289, whole genome shotgun sequence.
ACCESSION   DADVXN010000281 DADVXN010000000
VERSION     DADVXN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.289"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>468
                     /locus_tag="KB942_002971"
     CDS             <1..>468
                     /locus_tag="KB942_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBB6853312.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DADVXN010000282          467 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.290, whole genome shotgun sequence.
ACCESSION   DADVXN010000282 DADVXN010000000
VERSION     DADVXN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.290"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..318
                     /locus_tag="KB942_002972"
     CDS             <1..318
                     /locus_tag="KB942_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853313.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          183 a           67 c           64 g          153 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt
      361 gacgctcccg acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt
      421 attaatgaaa agcttggtag cacggttatt catatgctaa tttattt
//
LOCUS       DADVXN010000283          463 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.291, whole genome shotgun sequence.
ACCESSION   DADVXN010000283 DADVXN010000000
VERSION     DADVXN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.291"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>463
                     /locus_tag="KB942_002973"
     CDS             <1..>463
                     /locus_tag="KB942_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853314.1"
                     /translation="GTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQ
                     PITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKY
                     TWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          189 a           68 c          108 g           98 t
ORIGIN      
        1 aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac
       61 cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag
      121 aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac
      181 tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat
      241 tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata
      301 ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg
      361 ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag
      421 acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DADVXN010000284          437 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.292, whole genome shotgun sequence.
ACCESSION   DADVXN010000284 DADVXN010000000
VERSION     DADVXN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.292"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>437
                     /locus_tag="KB942_002974"
     CDS             <1..>437
                     /locus_tag="KB942_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6853315.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV"
BASE COUNT          136 a           82 c          105 g          114 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      301 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      361 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      421 tattctttaa cagacgt
//
LOCUS       DADVXN010000285          413 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.293, whole genome shotgun sequence.
ACCESSION   DADVXN010000285 DADVXN010000000
VERSION     DADVXN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.293"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..390
                     /locus_tag="KB942_002975"
     CDS             <1..390
                     /locus_tag="KB942_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853316.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DADVXN010000286          396 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.294, whole genome shotgun sequence.
ACCESSION   DADVXN010000286 DADVXN010000000
VERSION     DADVXN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.294"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(91..>396)
                     /locus_tag="KB942_002976"
     CDS             complement(91..>396)
                     /locus_tag="KB942_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853317.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          107 a           95 c           78 g          116 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggt
//
LOCUS       DADVXN010000287          368 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.295, whole genome shotgun sequence.
ACCESSION   DADVXN010000287 DADVXN010000000
VERSION     DADVXN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.295"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
BASE COUNT          130 a           59 c           61 g          118 t
ORIGIN      
        1 catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga
       61 cttatttttg attttatagc actgatacta taaaatatag atatcatcat attacacaat
      121 cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg
      181 ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact
      241 ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc atgaatcggc
      301 tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac actggaggat
      361 ttttcatg
//
LOCUS       DADVXN010000288          342 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.296, whole genome shotgun sequence.
ACCESSION   DADVXN010000288 DADVXN010000000
VERSION     DADVXN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.296"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
BASE COUNT          123 a           37 c           62 g          120 t
ORIGIN      
        1 ctatgttata ctctattagg aaatataaga aggagtataa aaatgaacaa acataataga
       61 aaaataacga ataacaaaga actgatcttg ttactaatag ggatttgggg tatcttttta
      121 ctctttaata tgtatatgcc gactatgaga gcggatgacg tagtttatgc aagtagatta
      181 gatgaacttg gctatttggg tgcttcgata gaacattata agacatggag ttcaagaatt
      241 attatagaac tattcttaat gttcttttct aaacacttta tgctatggaa attattaaat
      301 tctacgatta tgttaggaat tgtagtatta ctttgcaagt at
//
LOCUS       DADVXN010000289          337 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.297, whole genome shotgun sequence.
ACCESSION   DADVXN010000289 DADVXN010000000
VERSION     DADVXN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.297"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            4..>337
                     /locus_tag="KB942_002977"
     CDS             4..>337
                     /locus_tag="KB942_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853318.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKA"
BASE COUNT          141 a           37 c           60 g           99 t
ORIGIN      
        1 gacatgatga ttatggaaga aatagaatgg aataatctga ctcaaaataa taaaaataat
       61 tctggatgtg gaattatctt tatgtttgtt atagttttgg gaatagtagt acttttaata
      121 actgtcaaga tagtagatta ctttgcgaat actcctgaag aacaaataca gagaaaactt
      181 cctgagtttg aaagagtacc accttatgat ccaagagata attcatttca agtaaaaaaa
      241 ttaaaagagt ctccaaatga aatgtggttt gaagttacag ttgaatcaag cgacttcaaa
      301 gaaaaaaaga aagaatacaa ttatattgta aaggcaa
//
LOCUS       DADVXN010000290          331 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.298, whole genome shotgun sequence.
ACCESSION   DADVXN010000290 DADVXN010000000
VERSION     DADVXN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.298"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            105..302
                     /locus_tag="KB942_002978"
     CDS             105..302
                     /locus_tag="KB942_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBB6853319.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          119 a           34 c           70 g          108 t
ORIGIN      
        1 tattcaggaa ttattttgct acgtttcaag aaggaattgt tttttgaaat ttaatgaatg
       61 ttagaataat agtctaaaaa cattaattta ggggtgatta gattatgaaa catttaaaaa
      121 ttttgtctat taaagagaca caacttatct atgggggtac cactcatagt ggaaaatatt
      181 atggaaatgg agtgtattgc actaaaaata aatgtacggt cgattgggcc aaggcaacta
      241 cttgtattgc aggaatgtct ataggtggtt ttttaggtgg agcaattcca gggaagtgct
      301 aaaatgaaaa aaaatgctaa gcaaattgtt c
//
LOCUS       DADVXN010000291          322 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.299, whole genome shotgun sequence.
ACCESSION   DADVXN010000291 DADVXN010000000
VERSION     DADVXN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.299"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..156
                     /locus_tag="KB942_002979"
     CDS             <1..156
                     /locus_tag="KB942_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBB6853320.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>322
                     /locus_tag="KB942_002980"
     CDS             175..>322
                     /locus_tag="KB942_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6853321.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYE"
BASE COUNT          132 a           26 c           64 g          100 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aa
//
LOCUS       DADVXN010000292          321 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.300, whole genome shotgun sequence.
ACCESSION   DADVXN010000292 DADVXN010000000
VERSION     DADVXN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.300"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(69..321)
                     /locus_tag="KB942_002981"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="KB942_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DADVXN010000293          311 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.301, whole genome shotgun sequence.
ACCESSION   DADVXN010000293 DADVXN010000000
VERSION     DADVXN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.301"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
BASE COUNT          121 a           45 c           49 g           96 t
ORIGIN      
        1 ttttctcact ttttttcaaa aatatggtat gcttcttagt gaattcaaat ataaaagagt
       61 ttaaagcgta atacacttat ggggaagtgg tttggggtgc gctttaaact cttctttatt
      121 attatctcac agcatgctta aaaggtctat ggattaagtt atgtaaaaaa gtgcctcttt
      181 aaacgtccta aaatccaaac aaggatagca taatgtacac tatcgttgat ccagaaaaca
      241 ggtaaataaa ttttgcaaaa gcaataagtt aaaaacatta caaaaaagac aaggatatca
      301 catcaagaaa a
//
LOCUS       DADVXN010000294          305 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.302, whole genome shotgun sequence.
ACCESSION   DADVXN010000294 DADVXN010000000
VERSION     DADVXN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.302"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB942_002982"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB942_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBB6853322.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DADVXN010000295          283 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.304, whole genome shotgun sequence.
ACCESSION   DADVXN010000295 DADVXN010000000
VERSION     DADVXN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.304"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            1..283
                     /locus_tag="KB942_002983"
                     /pseudo
     CDS             1..283
                     /locus_tag="KB942_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DADVXN010000296          282 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.305, whole genome shotgun sequence.
ACCESSION   DADVXN010000296 DADVXN010000000
VERSION     DADVXN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.305"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..>282
                     /locus_tag="KB942_002984"
     CDS             <1..>282
                     /locus_tag="KB942_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBB6853323.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DADVXN010000297          275 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.306, whole genome shotgun sequence.
ACCESSION   DADVXN010000297 DADVXN010000000
VERSION     DADVXN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.306"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(58..142)
                     /locus_tag="KB942_002985"
     tRNA            complement(58..142)
                     /locus_tag="KB942_002985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="KB942_002986"
     tRNA            complement(163..233)
                     /locus_tag="KB942_002986"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
BASE COUNT           84 a           61 c           57 g           73 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctataaaata aagaataaat tatag
//
LOCUS       DADVXN010000298          267 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.308, whole genome shotgun sequence.
ACCESSION   DADVXN010000298 DADVXN010000000
VERSION     DADVXN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.308"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(35..>267)
                     /locus_tag="KB942_002987"
     CDS             complement(35..>267)
                     /locus_tag="KB942_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6853324.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaata
//
LOCUS       DADVXN010000299          256 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.309, whole genome shotgun sequence.
ACCESSION   DADVXN010000299 DADVXN010000000
VERSION     DADVXN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.309"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            <1..137
                     /locus_tag="KB942_002988"
     CDS             <1..137
                     /locus_tag="KB942_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6853325.1"
                     /translation="KYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK
                     "
BASE COUNT           92 a           55 c           43 g           66 t
ORIGIN      
        1 gtaaatataa ggaggttcca aagcatcaac acaaacgtgc actcgtccta accgcaagaa
       61 aatttacgcg tctggtggat acgctactac gtaaacacca actctatacg ccaccaagga
      121 gtgtgataga gaaataacaa tccgttattg acgcaaatcc ttgaatcaac ccgaaaaaaa
      181 tttgattttg atcgggtcta gtttcgtgcg cttcaaaaca tattactcaa taaagaaaat
      241 cttaaatttc atcttg
//
LOCUS       DADVXN010000300          253 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.denovo.310, whole genome shotgun sequence.
ACCESSION   DADVXN010000300 DADVXN010000000
VERSION     DADVXN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.denovo.310"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            28..>253
                     /locus_tag="KB942_002989"
     CDS             28..>253
                     /locus_tag="KB942_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBB6853326.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DADVXN010000301        31404 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.019, whole genome shotgun sequence.
ACCESSION   DADVXN010000301 DADVXN010000000
VERSION     DADVXN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.019"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            3..845
                     /locus_tag="KB942_002990"
     CDS             3..845
                     /locus_tag="KB942_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6853327.1"
                     /translation="MFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEAVEGFGYQ
                     NQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDELPTMTEL
                     WPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAYRLKLTGT
                     YVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEAISDDAQM
                     ILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQENGVTKLV
                     KKSN"
     gene            950..1423
                     /locus_tag="KB942_002991"
     CDS             950..1423
                     /locus_tag="KB942_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6853328.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            1519..2406
                     /gene="xerD"
                     /locus_tag="KB942_002992"
     CDS             1519..2406
                     /gene="xerD"
                     /locus_tag="KB942_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBB6853329.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            2425..2796
                     /locus_tag="KB942_002993"
     CDS             2425..2796
                     /locus_tag="KB942_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6853330.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            2766..3563
                     /locus_tag="KB942_002994"
     CDS             2766..3563
                     /locus_tag="KB942_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBB6853331.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            3547..4116
                     /gene="scpB"
                     /locus_tag="KB942_002995"
     CDS             3547..4116
                     /gene="scpB"
                     /locus_tag="KB942_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBB6853332.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            4122..4838
                     /locus_tag="KB942_002996"
     CDS             4122..4838
                     /locus_tag="KB942_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6853333.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      4920..5045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5168..5779
                     /locus_tag="KB942_002997"
     CDS             5168..5779
                     /locus_tag="KB942_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6853334.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            6157..7680
                     /locus_tag="KB942_002998"
     CDS             6157..7680
                     /locus_tag="KB942_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBB6853335.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            7917..9257
                     /locus_tag="KB942_002999"
     CDS             7917..9257
                     /locus_tag="KB942_002999"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBB6853336.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            9410..10147
                     /locus_tag="KB942_003000"
     CDS             9410..10147
                     /locus_tag="KB942_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBB6853337.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(10312..10533)
                     /locus_tag="KB942_003001"
     CDS             complement(10312..10533)
                     /locus_tag="KB942_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBB6853338.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            10564..11595
                     /locus_tag="KB942_003002"
     CDS             10564..11595
                     /locus_tag="KB942_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6853339.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            11582..12985
                     /locus_tag="KB942_003003"
     CDS             11582..12985
                     /locus_tag="KB942_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBB6853340.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            13051..13686
                     /locus_tag="KB942_003004"
     CDS             13051..13686
                     /locus_tag="KB942_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6853341.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            13735..14415
                     /locus_tag="KB942_003005"
     CDS             13735..14415
                     /locus_tag="KB942_003005"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB6853342.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            14550..15779
                     /gene="rpsA"
                     /locus_tag="KB942_003006"
     CDS             14550..15779
                     /gene="rpsA"
                     /locus_tag="KB942_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB6853343.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            15961..17271
                     /gene="der"
                     /locus_tag="KB942_003007"
     CDS             15961..17271
                     /gene="der"
                     /locus_tag="KB942_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB6853344.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            17514..17789
                     /locus_tag="KB942_003008"
     CDS             17514..17789
                     /locus_tag="KB942_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6853345.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            18024..19070
                     /locus_tag="KB942_003009"
     CDS             18024..19070
                     /locus_tag="KB942_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853346.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            19072..20331
                     /locus_tag="KB942_003010"
     CDS             19072..20331
                     /locus_tag="KB942_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6853347.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            20337..20918
                     /locus_tag="KB942_003011"
     CDS             20337..20918
                     /locus_tag="KB942_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6853348.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(21000..21875)
                     /locus_tag="KB942_003012"
     CDS             complement(21000..21875)
                     /locus_tag="KB942_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6853349.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            22023..22355
                     /locus_tag="KB942_003013"
     CDS             22023..22355
                     /locus_tag="KB942_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6853350.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            22467..23675
                     /locus_tag="KB942_003014"
     CDS             22467..23675
                     /locus_tag="KB942_003014"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6853351.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            23829..24284
                     /locus_tag="KB942_003015"
     CDS             23829..24284
                     /locus_tag="KB942_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853352.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            24646..24921
                     /locus_tag="KB942_003016"
     CDS             24646..24921
                     /locus_tag="KB942_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853353.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            24934..27012
                     /locus_tag="KB942_003017"
     CDS             24934..27012
                     /locus_tag="KB942_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6853354.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            27172..29058
                     /locus_tag="KB942_003018"
     CDS             27172..29058
                     /locus_tag="KB942_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6853355.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            29070..30017
                     /locus_tag="KB942_003019"
     CDS             29070..30017
                     /locus_tag="KB942_003019"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6853356.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            30037..30564
                     /locus_tag="KB942_003020"
     CDS             30037..30564
                     /locus_tag="KB942_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6853357.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30627..31280)
                     /locus_tag="KB942_003021"
     CDS             complement(30627..31280)
                     /locus_tag="KB942_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6853358.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT        10591 a         5291 c         6689 g         8833 t
ORIGIN      
        1 ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc caaaaaaacg
       61 gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa gcagttgaag
      121 gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt cctaaaagcc
      181 gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta ggtgcgtttg
      241 tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg ccgacaatga
      301 cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt gatgcaaaag
      361 accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc aaatatggaa
      421 cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa ttaacaggta
      481 cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa cgatataatg
      541 aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca gacggtgtac
      601 tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca caaatgattt
      661 taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa tcggatccag
      721 aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca ttaggtaatc
      781 ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc aaaaaatcta
      841 attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat taacatagtg
      901 ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa tggtcatgaa
      961 tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt ttaagctgac
     1021 accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc atttatctgc
     1081 agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg ctactgttta
     1141 tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt ttaatgatgg
     1201 attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc atttactatg
     1261 cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag tggagcaagt
     1321 agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc atggaatctg
     1381 tcaggaatgc tatgaaaaag aaaaaagaaa ccagtccgtc tgagtgtcta gacggctttt
     1441 tctttgctca gattatggaa aaagactatc agaatgaagc ggtatctgat atgatagaga
     1501 ggcatgaggg ggattattat gaaagagcaa atcaacgatt atctccacta cttgaatatt
     1561 gaacggggat tgtccgccaa tacacgacaa agctatgaac gtgatttgga acagtacttg
     1621 cattttctac aggagcagaa aattgcttca tggcaggatg tcgatcgctt tttagtcatc
     1681 cgttttctgg agaagttgca tgaagaaaaa aaagcgacag caactgttac tcggatgatc
     1741 tcaagtctca gaagattcca tcagttttta cggcaagagc gtatcactga tcatgatccg
     1801 atgcaacata tcgatacgcc gaagaaagca cagaaattac caagtacatt gactctgaca
     1861 gaagtagaaa agctgattga agcacctgat acgacaaaaa cgttagggat aagagatcgt
     1921 gcaatcttgg aagtcatgta tgctactggg atgcgcgtca gtgaattgat tggattgaaa
     1981 ttgggagatc ttcatctttc tcttggactg gtacagacag ttggaaaagg agataaagag
     2041 cggatcattc ctttaggtga ttatgcgatc caatggctgg aacgctactt agacgaagca
     2101 cgtccattac ttgtaaagga cgcatctgaa atgcatgtat ttgtcaataa tcatgggaaa
     2161 ggtctttctc ggcaaggaat ctggaaaaat ctgaagcagt tagtacgaga ggctgggatt
     2221 tacaaagaag taacaccaca tacgctgcgg catagctttg ccacacatct gctagaaaat
     2281 ggagctgatt tgcggacagt acaggaattg ttaggtcacg cggatatctc tacgactcag
     2341 atttatacac atatcacgaa gaaaagaatg acggatgttt ataagcagca ttttccaagg
     2401 gcttagtcat aggaaggtga gaagatgtta acacattacc ggaaagaaaa ccgtaaaatt
     2461 gctatgggac tattgagttt ccataagaaa ctgacaggta aaacttcttt gttgaaggag
     2521 attgatacgt acgaatctga agacgattat gaactttttt tctttacgcc agaaggttca
     2581 accaacattc aagggattat tggtatttac tgggcaacga acgatcagct gatcatccat
     2641 gacatttctt tgaatccgtc ttatcgcggt gagaatatgg gctttcaaat gttagatgaa
     2701 ttacaggaaa agtacccgga tgtctccatc cgtgcaactg aaatcacaga gccttacatt
     2761 ggaaaatggc tagcaaagaa aaagaagaaa gagtgaatga gttgacggag atcaatatta
     2821 aattagatgt attcgagggt cctctcgatc tgctgcttca tctgattcag caaatggaaa
     2881 tcgatatata tgatataccg atcgctacga tcacggagca atatatgaat tatatccata
     2941 caatgaaaac actcgagcta gctgttgcag gagaatactt agtgatggct gctacattga
     3001 tggcaatcaa aagcaagaca ttgcttcctg tccaagaatt cgtcacagac gatgaagaat
     3061 tatgggaaga agatcctcga gaagaattgg tcactcagct tttagaatac cgtaaatata
     3121 aatatgcagc tgaaagactg acagaaaaag cagaggaacg aagtctttat tacacaaaag
     3181 aacctatgaa catcgatggg ttagcggatg ctgaaaagcc gttgaagcga ggacaagtca
     3241 aaaaaatcga tttgatccgt gccatgcaga aaatcttaga acgtaagaaa ttttctcaaa
     3301 aagtggaaaa aacgatcacg cccgatgaca caacgatcga agaacgaatg gtatttatcg
     3361 aagagcgtat aaaaagaaat gcgcatggct gtactttcga ttcttttttt gagtcacctt
     3421 ctaaaagtga agttgtcact acttttatgg ctttgctaga attgatgaaa aaagggcaga
     3481 tcattgcgca acaaaaagaa aactatgaat caattatgct ttatccagca gtaggagata
     3541 ccgaacatga cgaaattaag tgaacttgaa gcccttcttt ttattgttgg cgaggaaggg
     3601 atcagtaatg aagaattaag cggactgtta aatctcagcg aagcagaagt cacaagttta
     3661 gtgaaagaat taaaagaatc ttacactcaa aactcgcact cagctttgca tatttttgaa
     3721 acggaaagcc attatgtatt aacgactaaa aaagagctgg ctcccttact taagcgatat
     3781 gcgcaaggga catcaaacac attatctcaa gccgccgtcg aaaccttagc aatcatcgct
     3841 tataaacagc caatcacaag agctgaaatc gagcagattc gaggagttca ggtttcagga
     3901 gcggtacagc gtctagctgc ccataaactg attgaagaaa aaggtagagt agaaggacca
     3961 ggtcggccga tattatatgg aacaactggc tacttcttag attattttgg tttgaaatca
     4021 ttagaagaac tgcctgatac acaagaaatg gaaatttcac ttgaagaaga cgtgtcacca
     4081 gatctattct ttgatggaat gaataaagga gaataattgt aatggagaga ttgcagaaag
     4141 taattgccca cgctggcatc acttctcgtc gaaaagcaga agaattcatc cttgcaggac
     4201 gtgtaaaagt aaacggacaa gttgtaaggg aattagggat aaaagttggg aaacatgaca
     4261 ctgtggaagt ggatgaagtc ccaatttatc aagaagaata cggttactat ttattgtaca
     4321 aacctaaagg agttatttct gctgttgcag atgacaaagg aagaaaagtt gttacggatc
     4381 ttttgccgat ggtaaaagaa cgtatctatc ctgtaggtcg actggattac gatacttcgg
     4441 ggatcttgct actgacaaat gatggagatt ttgctcagcg cctgacacat ccaaaacatg
     4501 aagtggataa agtttacgtt gcaaaggtaa aagggatcgc tacaaaatcg atgcttgcac
     4561 ctttgacaaa aggaataaag atcgaaggac gaaaaacatc tccagctaaa ttccaaattt
     4621 tatcagtgga tcctaagaca aaccacagta tcgtggaatt gacgatccac gaaggacgta
     4681 atcatcaagt gaaaaatatg cttcaagcag ttggtcttcc tgtgcaaaaa ctaaaacgtg
     4741 aaaaatacgg agatctgact ttacaaggcc tacgtcctgg ggattaccgt gaactgaaca
     4801 aaaaagaagt cagcagtttg ctgaaccact caaaatgaac acttactttt tataagtgtt
     4861 agatgttgcg ttttttgaat agatatgtat aatgaatatg taaacaaaaa ttatataagg
     4921 ttcgtcttca ggggcagggt gaaattcccg accggtggtt atagtccacg acctattttg
     4981 caaaaaaata gctgaatcgg tgaaattccg ataccgacag tatagtctgg ataaagaaga
     5041 tagagcttat ttgacgacgc ttgatcaaat aactctaact ctattttccc tggaaaatag
     5101 agttttttca tttggagaga aacgtcaaaa agttcgctga agaggagtcc taacaggagg
     5161 atttcaaatg aagaacaccc gtgtacagaa aatggttgca gtagcgatgt ttgctgcgat
     5221 cgcattagtt ttacagtatt ttgcttttcc gatcatgccg gcttttagtt ttttgaagat
     5281 cgattttagt gatatctcgg tattgatcag catgttctta ttcggtccag cggccggagt
     5341 tgcaacagcg ttcttgcgtt cagtattaca tctgctgact acaggttttt ctccggataa
     5401 tttggtagga gacactgcta gtttcttatc aacaacgatc tttacattcc cgatttatta
     5461 tttctttagt gtaaagaaac agcataaagg aagaaacaaa tttttaggtg tcattacagg
     5521 aaccatcgct ctaactattt ttatgagtat cgcaaattat ttcgttatta cgcctcttta
     5581 cttaatgttt ttagggctga acgcaacaca gatgctgggc atgtctttag caaactatgt
     5641 actgattggg atcgttcctt tcaacttgat caaaggattc atcgtgagtg ctgtcttttt
     5701 agtgctgcat gcgcaacttc ttccatggtt aagtcgaaaa caacatcagt tcgaacaacg
     5761 tcatacagca gtcaaataaa tatcaaaaag atcaagaaat ttcttggtct ttttttttgg
     5821 aaaaaaatac aagacatgca ataatgtaaa cgttttattt gtttgttttg agtatttttt
     5881 tgatttatgc ataaaaatca atcaattctt tgcaaatgtg aaattcttct ccttttaaaa
     5941 gacgaaaatg tttgtttata aaaaaaagga aacgctacaa tgaaaaagaa gaaagcttta
     6001 aaaacctgaa aaaagaggag ggaataggga aaaacgaact ttttgaacaa aagttttaca
     6061 aaatgcgtgt tattattttt gttctgatac tctttttgat aaaataacat tagagccaaa
     6121 attgttgtct ctaacggaaa agaaaggaag aagaagatgg aacaaacaaa aaagaaaaaa
     6181 tttcaaatgc cttcagcgta tacggtctta tttattatta ttgcagtaat cgctgtactt
     6241 acttggttta taccagctgg gcactatagt gtagacgatg caggcaacat cgtcgctgga
     6301 tcatatgaac gagtacaaca aaatcctcaa gggctgtggg atattttcat ggcgccaatc
     6361 aatgggatgc taggtgtacc agcgggggag ctaactaaag aaacacctgc tgcaatccca
     6421 atctctttct tcattttggt tgttggtgga tttttaggta ttatcaacaa aacaggggca
     6481 ctagatgccg ggattgcttc agttgtgaag aaattcaaag gaaaagaaaa aatgcttatt
     6541 ccagtattga tgctgttatt tgcattaggc ggctctactt acggaatggc agaagaaacg
     6601 attccattct atgctttact gatcccagtc atgatggctg taggctttga tacagtagta
     6661 gctgtagcaa tcgtattagt agggtctcaa gtaggctgtc tagcttcaac ggtaaatcct
     6721 tttgcaacag gcgtagcttc acaagctgtt gggatttcga tgggtgaagg tattggtctt
     6781 cgattcttga tgttcgttat tttgttagct atttctattg tatatgtgta tcgttatgca
     6841 tcaaagatcg aaaaagatcc aacaaaatca ttagtataca accaacgtaa agaagatatg
     6901 aagcatttcg atattgaagc aatcggtcgt caagaagtaa tcacgaaaaa acaaaaacat
     6961 gttaatgtat tgttctttat tacttttggt atcatgatca tcagtttgat tccttggaca
     7021 accttgaatg cgaatttcac gatttttgaa tcattgacaa actggttaac aaaccttccg
     7081 gtactaggta cggttctagg taaaagcatg cttccattag gtgactggta cttcccagaa
     7141 attactatgt tattcttagt aatgtccatc gtagtagcaa tcacttatgg catgaaagag
     7201 tctgaatttg tctctgaatt tttagctggt gcttctgatt tgatcggtgt agcaatcgta
     7261 gtagctgttg ctcgtggtat ccaagtggtt atgaacaatg gattaatcac agacaccatc
     7321 ttacattggg gcgaacaagg attaagttca ttatcttcaa gtgtattcat cattattaca
     7381 tttattttct atatccctat gtcattcttg attccttcaa cttccggctt agctgctgca
     7441 acaatgggaa tcatgggacc tatgggtgaa tttgccggcg tcggtaagga tttagtaatc
     7501 actgcttatc aatctgcatc tggtttagta aacttaatta caccaacatc tgctatcgta
     7561 atgggtgctg tagcaattgc tcgtttcgat gtttcagtgt ggtggaaatt taccggtaaa
     7621 ttaattgcga tattatttgt agccatctgt gtgatcctag gtgtagcagc aatgttctaa
     7681 acctagacaa atacaggtac aaggtctatc ataagaatga agaaaccgat aaacggctat
     7741 cgataagcac gtggataagt caatagctcc attcagtgag tgttttttct ttcatcattt
     7801 gttaagaaca ttctgcttgc tgatggagca ggcttaaaac gtcattattt gagaagaaga
     7861 ggttgtgtcg gaggaatttg acatgaccct ttttaacata ttaggaggat aatatcatga
     7921 aaaaatttgt gacagaacaa catcatgaac aagcattaga atcattgagc gaattgatcc
     7981 gaattccttc cgtactagat gaagcagatt caggacaagg acatcccttc ggtaaaaaag
     8041 tgattgaagc tttggataaa gtattggaaa tcagtgaaaa gctaggattt agaacattta
     8101 aagatccaga aggctattac ggttattcag agattggctc tggagatgaa ttattcggta
     8161 tcttgtgtca tatggacgtt gttccagcag gggacgaaaa taattgggaa acaaaaccat
     8221 tcgatccaac tataaaagac ggctggttag ttggtcgcgg atcccaagat gacaaaggtc
     8281 cttctattgc ggcaatgtat gccgtcaaag ctttgattga tgctggcgtg gaattcaaga
     8341 cacgtattcg ttttatcttt ggtacagatg aagaaaattt atggcgttgt ttagaaaaat
     8401 ataacgaaaa agaagaagga attacgcaag gatttgcgcc agatgctgaa tttcctttaa
     8461 tttatgcaga aaaaggctta ttgcaagctt acttgacagg accaggaacg aacgagtttt
     8521 ctgtaaaagc tggaggagcg ttgaatgttg ttcctgatgc agctccttac tctggagaaa
     8581 agctttctga agtaaaagaa gcattgaaaa aacacgaatt cgatttcgaa gaacaaggtg
     8641 aaggcatcgt cgttcttggt aaaagtatcc acgcgaaaga tgcagcacaa ggagtgaatg
     8701 ctatttctcg tctggcaatt gctctttcag aagtatttga ttttggccca atcaatttct
     8761 taggaaaatt agttcaagaa aatgcaactg gtgaagctgt cgtgggcaaa acagaagatg
     8821 aacaatccgg cgagctaacg atgaactttg caagtttgga aatcacacca gaacaaacaa
     8881 aaatcggtgt agatatgaga atccctgtta cattcaagaa agatgattta gtagctaaat
     8941 taactaaaac agcagaaaaa tatgggttga cttatgaaga atttgacttc cttgattcat
     9001 tatacgtgcc actagacagt gagttagtga aaaatctatt ggggacatat cgcgatatta
     9061 ctggagacat gactgaaccg tttgtatcag gcggcgctac ttttgctcgt acgatgaacc
     9121 agtgtgtggc attcggtgcg atgttccctg atactcctga ctttatgcac caggcgaatg
     9181 aacgatggga attaagtagt atgtataaag caatggaaat ttatgcagaa gctgtgtatc
     9241 gtttatgtgc taaataaaaa taaacataca aaaagggcgg atattcaaat ggatattccg
     9301 tcttttttta tttgatgctg gaaagtttgc atttctctct tattttttta aggtataatc
     9361 atacttgatt aagaaaaagg ggtcactcag atagaaaagg tgggagaaag tgaaaatatt
     9421 tagacgaata ctaattggct tagtcatcat tttaggatta ctcggtggag ccggttatta
     9481 ctatatacag caaaatacgt acgaaccatc gtcagaagcg caagtgttgg taaaagaagg
     9541 aaaaaacaaa gaagattact tattttttga aggagagaaa aagaatcatg gaaagtctcc
     9601 attacttatt ttttatcctg gtgctctcgt agagccagaa agttatagta tttgggcgca
     9661 agatttagca aaaaacggct tttcagtcgc tattgtcaaa atgccacttg atctagcagt
     9721 gatgggcgga aacaaagcgg ataaggtcaa agaagatttt ccaaattccg attatgtgat
     9781 cggtggccat tcattaggtg gcgtaatggc aagtcgttat gtcaataaga atcattcaga
     9841 caaacattta aaaggggtat tcttcatggc aagttatccc gacaaaaaag gaacattgac
     9901 agaatcagct gttcctgttt tatcgatcac ggcgacaaaa gataaagtat tgaatcagga
     9961 agcttatcaa gaagctaagc agtatttacc ggaaagaaca gagtatgtat caatcaatgg
    10021 cggaaatcat ggaggttttg gcagctatgg tgagcaaaaa ggagatggag aggcagaaat
    10081 ttctaacgca gaccagcaaa ctgtgatcgt caatacattg gaaaactggc tgaaaaagtt
    10141 gaaataatat aaatgggtcc tatagggtcc gtgaaaaaac tagtcaatta actatagata
    10201 gcaaatgaat ctttcaaaat aagtcgggat ttcacggaac aagagaaaca gatcccggct
    10261 tattttcaag tttagagaac ttagtgtacg tttttaaagt ttcgtttata tttatatttt
    10321 attttctaat aaaatcgctc gaactggaca tttttgatac gcttttttta catatgtttc
    10381 ttcttccggc atcacgaact gttgatgagc ctctggttct tgggcaaata aaacgattcc
    10441 ttcttcatca taatcgaaaa tatctggcgc ttcgatttga caaagaccgc atgcaataca
    10501 gcgttcaggt acaagtttac atagtagtga catttctaac catcctaact taaggagaat
    10561 aatatgattg tttttatttt atcgctattt tcatcaggac acaagctgcg gattagttct
    10621 ctttaccaac tccttgtggg aaaacgaacg acttcagtac ttatttatgg atttacccac
    10681 gaacttttgt ttatccataa cagttttcca gaattgaagc aggataagtt ttatcaaatt
    10741 ttgcaaaaaa ttgcgcagca aggttggatc gaaataaatg aaaatgaagc aaagctaacg
    10801 cctgcagggg ctgatatgtt gtccgaacat caaatagaac atactggttt gcgttttgac
    10861 cgctatggca gaacagggga aactagttgg cggctcatta aatttgctgt tcaagtaatt
    10921 tctaatctgg caacgggtat tcaagactat cttccagcag agacaagtcc tttttatact
    10981 ttccaactaa aaaaatggct tagtgaaagc caattgccac gtgaaatttt gattgatact
    11041 gcgtatgaga gtttggtgca gctgttttcg gagataccag aagaagcagc agatttttta
    11101 gctaaccaat tatctggaaa tgagcgaaca gggctgcttc cttatcagct ggcaaaaaac
    11161 aatgatgagt cagcggttta tctgcaacaa agcagatgta tccacctttt gttagcacaa
    11221 atcgaaaaac ggccagattc gctttggcac gctttgatcg attcattatt acagcaaaat
    11281 ttcaatcaaa gcatgatgat caccaagcag atgtttatga atggtcagac aattgaccag
    11341 atcatggcaa tccgtcattt aaaaagagga acagtaacag atcatcttat tgagtgggcc
    11401 ttgttttttg atgattttcc atacgagtgg attctctctg cagaaacaat cgaacgtttg
    11461 gaaccaaata aggattccgt gcgagagtgg cgctttagcg aatggaatgt agatggacaa
    11521 ttagattatg gagaatttcg tttgtatcaa atctatctat tgagaaagga agcgatacag
    11581 aatgttaata aatgaattaa aaaaatattt tggatttgat tcatttcgtc ccggccaaga
    11641 agaaattatt caggcgttgc ttgatggaga agatactctc gctattttgc ctacaggaac
    11701 gggaaaaagc ctttgctatc aattgacggg gtatttaatg gaaggtctgg tcatcatcgt
    11761 gtcaccgctg ctttctctaa tggaagatca agtgacgcaa ttacaaaaac gaggggagaa
    11821 acgagtagct gctttcaata gtttactttc gcaatctgaa agaagatacg tcctccgtca
    11881 tctatcgcaa tataaatttt tgtttttgag tcccgaaatg ttgactaatc cttctctttt
    11941 agagcatctg aaaaaacaaa atattgcgtt atatgtagta gatgaggctc attgcgtgtc
    12001 tcaatgggga gttgatttcc gtccagaata tcaacagtta ggaaaaatcc gaaagctttt
    12061 aggaaacccg gtcacactgg cactaacggc gacagcaaca gatttagtag caagagatat
    12121 acgtcatgta ctatttgatc gaccaccaaa agagatcaga caatcagtca atcggcaaaa
    12181 tatctcgctt tttgttcgga aaacgcagca aaaagaacaa gaacttgaac acttcatgga
    12241 aagagcacaa ggagcggcaa tcatttattg tgcaacaaaa aaagaagtcg aacgattgta
    12301 tcatttattc agggaacgtt ttacagttgg atactatcac ggtggtcttg atgcagctca
    12361 aagaagacaa cttcaacagc agtttgtaaa aaatcagctg cagtttctca ttgctacgaa
    12421 tgcatttggt atggggatcg ataaatctga tatccgttat gtggtccatt atgatttgcc
    12481 agacagtcta gaaaactatg tacaagaaat cggtcgagca ggcagagatc aggaagaaag
    12541 tgcagcaatt cttttttatc aaagtgggga tgaacgtatc cattatttct ttaatcaact
    12601 ttctagagaa cagcgacaaa gttttgagct ttatttggaa tatgccacag agcaagctcc
    12661 ctttgatgag ctgcaaaaaa aatggatgga gctgatccag cagtctgaaa aaccagaaaa
    12721 ttgggtggaa cggctcaaac gtcaggaaaa agaaaaagaa ttccgcctgc agcagatgct
    12781 tcgctatatt aatgaagaaa actgccgcag aaaattcatc ttagcttatt ttggcgaaaa
    12841 actctccgaa aagccgcaga attgttgtga tattgatgga gcaaaagcag aacttctgcc
    12901 aaagaggaag cttttttcta agactgaaca acttcattgg ggaacgattt tactaaattt
    12961 atttaaaaaa aacatggaac attgattttt taggacaaga cactttttcc tatggtataa
    13021 tacacacgat agagtttagg aggtaaaaca gtgagcagaa aagatagaca tcaatctaca
    13081 gaaaataacg aaacacaaga accatgggaa caaccaatct acgatacaga tgatgaagtt
    13141 tcatcaagaa gttctcagag acaacaaaag aaaggaaata cattatttct ttcgctattt
    13201 ttgattttac tggtcttatg tatcgctatt ccagcaggtg cttatttctg gatcagaaat
    13261 ggatcaagca acaatgcaac agcagcaact tcttctgaaa cttcgacatc agtagtagaa
    13321 agttctacaa gtacaacaaa agaggagtca actgagactt caacagtaga atctagtaca
    13381 atcgcggaag aatctactgt gtctcaagat acaggtgtaa cgtcagaaag cagtattgca
    13441 gcagagacaa caccgacttc ctcaagtgag gcaacttatc aagcagggga ttcaagcagc
    13501 aatgcagcag aaacaacacc ttcttcatca gctgcagccg gttcaacaac gactgttcaa
    13561 gcaggcgaag gtccaaaaca agtggctgag cgtgcaggga tcaccactga tcaattgttt
    13621 gaattgaatg gcttagatcc aaacaacttt atgctttacc ctggtcaaga attaagaatt
    13681 aaataataat agccgcgctt tgcggctttt tttctgagaa aaaggagtta tatcatgaca
    13741 caaatcagta ttgcaatcga cgggcctgct tcatctggga aaagtaccgt tgccaagata
    13801 ttagcaaaag aattgaatta tatttataca gataccggtg ccatgtatcg cgcagtaact
    13861 tatttagcta ttacgaacaa tgtatctttt tcagatgaag aagggctagt ttcgctgatc
    13921 aaaaagtatc ctatctcttt ttcgcaacaa gaagatggac agcatgtatt catagcatca
    13981 aaagatgtga ctttagaaat ccgtcaacca gatgtcacaa aagctgtttc tgaagtctct
    14041 gcacatggaa aagtcagaga agaattagtt gctgtccaac gaaaaatagg ggaacgcggt
    14101 ggcgttgtca tggacggaag agacatcggg acagctgtat taccgaatgc agaagtgaaa
    14161 atctttttag tagcaagcgt aagtgagcgt gcagaaagac gtttcaaaga gaaccaagaa
    14221 aaaggaattc cgactgatct tgaaacactg acgaaagaga tacaggaaag agaccattat
    14281 gattcaacaa gggacgtctc tccgctgaag caagcagagg atgctgttcg tatcgatacg
    14341 actggcaaat cgattccaga agtcgttgca gcaataaaag ctgttgtttt gaaaaaaggt
    14401 tatcttcttt tttaaattaa gaaaaaaaat gaaaaacaat tgttttttca aggaaaacga
    14461 acgaaatcgc tttatttttt ttgaataatc aattaaacta aaaacagtac cgtacttacg
    14521 gcagaatgtt ggttaggagg acaagtgtta tgacagaatt tgatcaaaat caagagaaca
    14581 accagtcaat ggaagacgca atgaaaagcg tacaagaagt aaaagttggc gatgttgtta
    14641 agggcgaagt tttagtcatt gaagacaaac aagtgattgt cggaatcgaa ggggctggag
    14701 ttgaaggagt cgttccggca aaagaattat caactttgcc ggtagaagat atcagcgaag
    14761 tagtaaaagt cggtgatgta ttagatttag tcgttatcag tacgatcggc aaagataaag
    14821 aaaacggcag ctatctactt tcaaaacgcc gtttagatgc taaaaaagtt tgggaagata
    14881 tcgaaagaga tttccaagag ggcaaggtca tcgaagcacc tgttacaaat gttgtcaaag
    14941 gcggtttagt cgttgacgtc ggtgtacgtg gttttgtccc tgcatcaatg gtggaagatc
    15001 attttgttgc agacttttca gactataaag gcaaaacatt gagctttaaa atcatcgaaa
    15061 tcgaaccatc agaaaatcgt ttgatcttat cgcataaagc ggtagtggaa aacgaaagag
    15121 aagaaaagaa aaaagaaatt ctttcaacta tccatgaggg agatgttctc agtggacgtg
    15181 ttgcacgtct tactgatttt ggcgcatttg tcgacctagg cggaattgac ggtctagtcc
    15241 atgtctcaga gatttcacat agtcatgtag caaaaccaag tgatgtattg gctgtcaatg
    15301 atgatgtaaa tgtcaaagtg ttgtctatca atcctgaaca agaacgtgtt tccttgtcaa
    15361 tcaaagacac acagcctgga ccttggtcag aaatcgatac aaaagctcca gttggcacag
    15421 tattagacgg aacagttaaa cgtttgacaa gcttcggtgc ctttgttgaa gtgttccctg
    15481 gagtagaagg gctagtccat atctctcaaa tttcacataa acatattgcg acaccacatg
    15541 aagtgcttca tgaaggtgac gaagtgaaag tgaaagtatt agagatccat ccagaagaac
    15601 atcgtattgc attgagtatc aaagctttag aagaaaaacc agcttcagaa gaagaaccaa
    15661 aaaatgttga gtcttatgaa ttaccagaag aaaatactgg atttactatg ggagacattt
    15721 taggtgatgc gttgaaagaa gaagtagaag aaaaagcaac cgatgattct gaaaaataat
    15781 tgattttgta tctgtgactg agatgaaagc agctgctttt gtctcagttt ttcttttgaa
    15841 aagcgattag tatttcaata aggaataaaa atacttcgtt taattgacaa tctatgcttg
    15901 aaagttggca atgcattcgt taaactaagt gagattggaa acttgtgagg aggaaataat
    15961 atggcaaatc caacaattgc aatcgttggc cgcccgaacg tcggtaagtc tacgattttt
    16021 aaccgcatcg ctggtgagcg tatctcgatt gtcgaagata ctcctggagt aactcgtgat
    16081 cgtatttatg caaaaggtga atggttaggt cgagaattca gcgtgatcga cacaggcgga
    16141 attgatcttg gcgatgaacc ttttatggat cagatcaagc atcaagcaga aatcgcgatt
    16201 gaagaagcag atgtaatcat ttgtgtagtc agcggacgcg aaggcgtgac agacgcagat
    16261 gagatggttg cgaaaatttt atacagaagt aataaaccag tgattttagc agtcaataaa
    16321 gtagataatc cagaaatgag aaacgatatc tatgaatttt attccttagg gctaggcgat
    16381 ccttatcctg tttcaggaag ccatggacta gggattggtg atattttaga tgaagcagtg
    16441 aagtatttct ctgaagagac tgaagaagag gacgacgaca cgattaagtt tagtttgatt
    16501 ggtcgcccaa atgttggtaa atcctctttg atcaatgcga ttttaggaga agaacgtgta
    16561 attgtttcgg atattgaagg aacgacccga gacgcaatcg acacccactt cgtttctgaa
    16621 agtggtcaga aattcttaat gatagataca gctggtatgc gtaaacgcgg aaaagtatac
    16681 gaaaatactg aaaaatacag cgtgatgcgt gcaatgcgtg ccattgaccg ttctgatatc
    16741 gttttgatgg tactgaatgc tgaagaaggg attcgggaac aagacaagcg agtagcgggt
    16801 tacgcccatg aagctggtcg tggaattatt atcgtcgtga ataaatggga tcttgtaaag
    16861 aaagaaacca atacaatgcg tgatttcgaa caagagattc gtgatgaatt ccggtatctt
    16921 gattacgctc caatcgtttt tgtttctgca gtgactaaac aacggttgga acgtctgcct
    16981 gaaatgatcg aacaagtaag tatgaaccag aatcttcgta tctcttcggc tgtgttgaat
    17041 gatatcatta tggatgctgt agcaatcaat ccaacaccaa cagataaagg aaaacgcctg
    17101 aagatttttt atgctacaca agtcgctgtc aaaccaccta catttgtcgt ttttgtcaat
    17161 gaagaggaat tgatgcattt ttcttatgaa cgtttcttag aaaatcaaat cagaaaagca
    17221 tttacttttg aagggacgtc catccgaatt atccctcgta ggagaaaata ggcattttac
    17281 catatcgacg tttttttttc aaaagaaaaa gacagtttcg tttgttacgt ttctatgact
    17341 aaaaaaagca tgtgaaaatt ggtaaaaacc ttgctattaa gcggttcata tgatatcgtg
    17401 ataacagaat attgaagtta atcaatattt ctttttaaac ttctggacca ctcagaaagt
    17461 tttaactaaa tgatatttct atcatatctc ttgtggagga ggtgaatcta tccatggcaa
    17521 acaaagcaga attaatcgaa aaagttgctt cagctactga tttaactaaa aaagatgcaa
    17581 ctgcagcagt agatgctgta ttttcaacta ttcaagatgc tttagctaac ggcgaaaaag
    17641 ttcaattaat cggttttggt aactttgaag ttcgcgaacg cgcagctcgc aaaggtcgta
    17701 acccacaaac tggtgaagaa atcgaaattc cagctagcaa agtacctgca ttcaaaccag
    17761 gtaaagcatt aaaagacgct gttaaataag acagttgaaa aagcgcttgt ggctctaggg
    17821 ctacaagtgc ttttcttttt atttcctagc agaaaaggaa taaataagag acagagattc
    17881 cgaatccgta tgtcatacat agaaaacaca tgtattttat ttaattttta tttatttgca
    17941 agttatgagg tgagctttgc tcttatcttt tctagaattt gcttactaat ggtaaaataa
    18001 aaataagaag aggagggatg cgtatggaac ttgattatca agtagaagat gcgttgacga
    18061 aagtgtttta cggagaaacg ctagtcagtc agctaagaga actttccatt gatcagccaa
    18121 tcttattttt aacgaatcag cgctattatg atttgtttgc agataagatc aacccgttat
    18181 ttgcaaatca agcaaacaat gactggtata tttgtgccaa cactcaatgc aatcatttaa
    18241 cagaattaaa gaacctgctt gatttcgtca aacgctatcc cgaaaatcaa tccatgttta
    18301 tcatcggatt tggaaatgaa gggatcatgg atctggcagg tttttttcaa aaacatacaa
    18361 cccttgattc atcactatgg atgatccctg tatcgatccg ttctctaagc cgagcattaa
    18421 caccgcagcg gacgattttg cagctaccaa atcgtgtagt gctgcaaacc atcaatctgc
    18481 cagaacgcat cgtttatgat caaacaatca gcgagcggca ggtggacgga aagcagattg
    18541 actttttgac ttttatctgt tgcggaatga tttgtgatca tcgttttttg caaaatctgt
    18601 ataaaaacta tccaaataaa aagcaattaa cgactcggcc gtttgccggg atgatcgaag
    18661 aaatgatcta tttttatcaa ttagaggcag aaaacctgtc aaattatggt aaactgtttg
    18721 aacaagcttt ttatctgacc gaaaatggcc atctactttc tgctagtatg aaaaaatttt
    18781 taggaatgat gtttcatttc gtgtggaatc tccaagcata tccttttgat ttccaactaa
    18841 aaaacttttt tgtgtggctg gacttgcttg gctatccaat ccactggctg gaacagattt
    18901 caaagatgga gtatttggaa aaagttcttt ttttggcaga aaaaagcaaa aaaatacttg
    18961 ttttagaaaa aataggtata atagacggat accaacatcc aaacgaaaaa gaattattga
    19021 gaacaatgac ggtatttcaa caaatcgtca gtgagataag agggaattga aatgaataca
    19081 aatagcgaaa aaatgttgca agctttatca gatgaagact tgtcacaagc acaaatcttt
    19141 ttagaaaaag cattaaaaga agacccagca gatatattag ctgaactagg cgaagaattg
    19201 ctggctattg gctttttgat cgaagcaaaa cagatttttg aacaactgat caaccaatac
    19261 ccagaaaatg acggactgaa catcccgtta gcagaaattg caatcgaaga taatgagatc
    19321 gacttagctt ttgaatacct ggaaaaagtg gataagcaaa gtgattacta tccgcaaagc
    19381 ttattggcga tagctgattt gtaccaagtt atcggaatcc ctgaagtgag tgaagcaaaa
    19441 ttgaaagaag cagctgggtt gcttcctgat gaaccattga tccagtttgc gctagctgaa
    19501 ttgtattttt cagttgatcg tttcaaagag gcagcagtgg tttatgaaac attgttggca
    19561 agcgggataa atgaaatttc aggaatctcg atgcaggaac gtttaggaca atccttaagt
    19621 atgcaaggag aattcgaagc agctatcgta cctttagaag aagcactgaa agaagaaagg
    19681 acagatgatc gactgttcca gctagctttt acgaatcttc aattgaaaga aaatcagcag
    19741 gcaatcaact atctggaaga acttcgagaa gttaatccgc attatcaatc gttatatcta
    19801 tatctaggac aagctcttca agaagaagaa atgattgaag aagcacaaac agtgttagaa
    19861 gaaggaatca aagaaaatcc ttaccaagtc gaactttatc acctggcctc ggaaaatgct
    19921 ttccgtctgc atgatagaca gcgagcagaa gaacttttac tgaaagcttt agaagtagga
    19981 gacaaacaag atgaaacatt acttacttta agtaatcttt acttggatga tgaaagatat
    20041 gaagatgtga tacaagtgat caatcagatg gaagaaactg ctaatcctta tgcagaatgg
    20101 aacctagccc atgcttataa tgaattagaa gactttaccg ttgcagctgt acactatgaa
    20161 caggcttatc atgaactatc acatgaacca gatttcttga aagagtatgc ctttttctta
    20221 agagaagaag ggcagctgaa aagagcgcaa gaattattga cttattattt aacacttgaa
    20281 ccaggagata tggaggcact gtcattatta gatgacctaa cagagaggtg agaaaaatgc
    20341 taattgatgt acgggaaaag aagaattttt taacgtggat ggtcaatcat gtatctttta
    20401 gccgaagaga agttttttgg atcttgaatt atcttgccaa tcatgaagcg attttaagca
    20461 atgtccactt tgttgaaggg gcgaattttg cggatcgagg actgcagatc agtgatctat
    20521 ctgttgaagg ggaacccatg aaattgtttt tgcagggaaa agaatttacg gacacagacc
    20581 agatttttca tgaaatccgt ttgaattgga aaaagccttt atatgtggaa tgtatattcg
    20641 aaaatgcttg gcagacaaga gaatatctat ctatcttaga agacaatcca ttttcttctt
    20701 gggattcttc agtcagtgaa gaagatactg aattaattga acgatatttt caagaaaaag
    20761 aagaagaggc aaaactaaaa atgctttatg ctcaaatcga tcaagcacta gaagatggag
    20821 atagggatgc ttttttgaaa ttatctgatg aagttaaccg aaagatcctt ttgcagacac
    20881 aaaagaaaag taaccgggta agtgaacata acaattagtt aaaaatagta tagtactaaa
    20941 aaagacaaag gtcagctgga tgtggaatct ttcctcatag cctatctttg tcttttttct
    21001 tatagttttt tcagtaatct tgtctgtggc ttcaaataag taaagccttg cccttccact
    21061 tcatgcacat tgatgattga aaggaaagct ttttcgtcaa tctcgtggac gatcctctta
    21121 gcttcagtta attcactaga gctaacgacc atatagatga ttttttttga attaccagag
    21181 aatgcgcctt ctccagaaag gaaagtcaca cctctttgca gaccggtcat cagtagtggt
    21241 gcaatctctt cgcttttgtt ggagacgaca agtagacctt ttgcagaata tccgccatct
    21301 aagatcgaat caatcacacg actgaaaaca tacgaaacaa tcaacgtata catcatacgt
    21361 ttcaagtcga tataggttaa tgaagcaagt aagacgatga tatcaaatgc caatagggaa
    21421 cgccccatag tgatgccgta attttgttct aaaatccgag cgataatatc actgccgcct
    21481 gttgttccac ccatgcggta gacgatcccg cttcctacac ctgcaactaa acccgcaagt
    21541 agagaaacga tcagtagatc atgctgtaaa ttgatttcta gtggaaccct ttgccagatc
    21601 cataaaaaga atgacaaaga aagtgtacct agaacagtat aggccaagga acgtttaccg
    21661 aggatctttc ctcctagtaa aatcaatggg atgttgatta ataaagtcgt ataagctgga
    21721 tcaataccaa ataaagctct taagatcaag gtgatccctg agactccccc ttctgctaaa
    21781 tgattagcga tgttcaagta aacaagacca aatgcataaa tacttgttcc tataatgata
    21841 aaaagaactt cttttactgt ttttcgtttg accatatgaa ttccagcttt caattatgta
    21901 ttttcacaga atttccattt agaaattcct tcctaaatgt agcatggaaa ttcgattttc
    21961 tcaattgctc ttatctagga tttgcgttaa agttttggta agatagtttt ggagggaaaa
    22021 agatggataa aaagcgttct ttatacagta tgcagcagga agtggatgaa tatatccagc
    22081 aattcaaggt ggggtatttc tctccattag ctcagatggc tcgattaact gaagaagtag
    22141 gtgaattggc aagagaagtc aaccacactt atggagaaaa aagcaaaaag gcttctgaac
    22201 cagttaactc ggtagcagaa gaacttggtg atgttttatt cgttacgatg atcatggcta
    22261 attcattgaa tatcgactta actgaagttt ttgaaaaaaa catggaaaaa ttcaatcaaa
    22321 gagaccataa tcgctttgag cgtaaggacg agtaaacaag atgaagtggt cctggccttg
    22381 aagactctga aacggacttg tcaaacagtc accatgcaac tgagtcttta tctcaggaca
    22441 agaaacacgc aggaaaggtt ggaaaaatga aattaaaaca attacctctg gaataccaaa
    22501 aggcaattcc agttctgaaa aaattagaga atgcaggata tgaagcttat tttgtaggtg
    22561 gaagtgttcg tgatatcttg ctgaaccagc caatccatga tgtggatatc gcaacaagcg
    22621 catttccaga agaaatcaaa cagctttttc caaaaacgat agatattggg atcgaacatg
    22681 gaactgtgtt ggtattagaa aatgatgaac aatatgagat caccacattt cggacggaat
    22741 caacttatca ggactaccgt agaccagatc atgtcgaatt cgttcgttcg ttggcagaag
    22801 atttgaaaag aagagatttt acgatcaatg cgtttgcttt gaaagaagat ggagaaatca
    22861 tcgatttgtt cgatggtctg caagatctag aagaacagat cttgcgagct gtaggcaatc
    22921 ctcatgagcg gttccatgaa gatgccttac gaatgatgcg cgggttgcgt tttgtcagtc
    22981 aactagggtt caaattagag gaagaaacct tctacgccat caaagaaaac cacgagctat
    23041 taggtaaaat atctgtagaa cgtataaatg tggaattcat caagatgctc ttgggaaatg
    23101 aacgggccgc gggaatccat gcatttattg aaacagagtg ctacatttgc tgtcccggtt
    23161 tgaaagaaga aggcgaagca cttttacgtt ttgctgaatt accatccgtc ccattgaaag
    23221 aagagagcca agcatgggca ttattgatca gccaactgga tctaaaagaa aatgagattc
    23281 gttccttttt gaaatcgtgg aagtgttcta accaaatgat taaagacgtt caagcacttg
    23341 tccaaggact acgacaacga gaaactggag ctttggaacc attcgaattg tacttcttag
    23401 gcaaaaaaca tgcactgcaa gtagaagaat tgatgatcta ttttgacgaa acacctcaga
    23461 tggatgccgt aggagaatcc tatgatcgtt tacctatcaa gtcattaagc gaattagcag
    23521 tagatggtaa aatcttgcta gaagaaactg gaaaaaaacc tggtaagtgg gttagtgaag
    23581 ctttgcaatt agctgaaaaa gccgtcgtag aacaaagaat aaaaaatgat aaaaaacagg
    23641 ttttggattt tttatcgaaa gaaaaattga tttaaaaaac actagactgt atctgctcag
    23701 ctttgttggc tacttaaagt gggcagatac gtcattagaa gaaaaaacat aagatgtgac
    23761 cacttacata aatttgtgct aaactgaggt agtacaagaa taaattacat cataaaggag
    23821 tctttttaat gagtaaagaa atgacagcgc taaaatttta ttttagaaat ggagaaacct
    23881 ggacgatcaa ccgccgtcat atcggcgatc tttggatcaa acaaatcaca acaagttttg
    23941 gacgaatcaa tggcagtgaa tttgttgaaa tccatccgtg tgctggattt aaaatcgaaa
    24001 tcttccatga aggggacgca gttgcgacac atgatatcaa cctaggcgga ctggaaatgg
    24061 gcatgtttaa tcgcgcattg aaatacgaag atatcgagcg aatggaaatc ctttaccgaa
    24121 atggcacacc tgatcttgtg tacttcccat atctagataa aggaacagaa ggattagaca
    24181 atcaatatca atcaacaaaa atcagcgaaa aaacaggtaa cttgtatatc gtgatcaatc
    24241 cagatcaacg tgtagaagat gtatacgggg aatttttcga ataaatcgat ttttacgcaa
    24301 aaatgaggct gtgaaagaga tgtgtcttat tactactgat aggctgtctc ttccacaccg
    24361 tttattcaga ataagggact gtgacaagat ttttgtcgca gccccatttt tttgttgtga
    24421 taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga aacttttatc
    24481 tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg ttgaaaaatt
    24541 tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc atccaaaaag
    24601 aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag agagagtttt
    24661 gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga tgaccgccgt
    24721 caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt ggaacgtctg
    24781 aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga ggggaaccaa
    24841 acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt tttaaaaatg
    24901 cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt agttattgta
    24961 tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat gattccagct
    25021 ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg acaatcaatc
    25081 gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt tagagaagga
    25141 gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt cttggcattt
    25201 ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct gttgccaagt
    25261 gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga tgcggtagca
    25321 gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat tttggaagga
    25381 gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct tggtgcgctg
    25441 atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc tagtattggg
    25501 ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca tttgatcgaa
    25561 aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct tccttttcta
    25621 gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt ggcagcaggt
    25681 attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt gtcgagcttg
    25741 tcccacagta catggacaac tattgctttt acactgaatg cattagtatt tatattttta
    25801 ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata tccaaacggt
    25861 cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag attcatttct
    25921 atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca gttgaacgag
    25981 atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac gatttttatt
    26041 ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt gtttctgacg
    26101 gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat gctgacggaa
    26161 acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga ggtcgttgaa
    26221 gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt agccactgaa
    26281 gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga tttaaccgat
    26341 gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga acgggatgga
    26401 ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt ttactcccgt
    26461 tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat cggcttttgg
    26521 ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt ttggcaaaga
    26581 agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat cagagagaca
    26641 tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga tatttatgat
    26701 gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa aatgaactcg
    26761 gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa aaaaatgcta
    26821 agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga aaaaatcacg
    26881 acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta tacgatgaac
    26941 aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa aaaaacgagt
    27001 ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca tatcagaaca
    27061 ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac caaaaaaatt
    27121 ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa catgttgaaa
    27181 gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt tgaccagatt
    27241 tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg gacgggtaaa
    27301 acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg gcaaacagtg
    27361 ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt ttccgatgaa
    27421 ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac tgttcgagca
    27481 tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt gcaaaaacgt
    27541 tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga taccaacgca
    27601 aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag tactttatca
    27661 ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc cgatttacta
    27721 ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt ggaaaactat
    27781 ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt tctagataga
    27841 gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa aggaaattat
    27901 gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca agaagaaaag
    27961 cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca ggcacgtggc
    28021 accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt gtaccaagta
    28081 aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa aaaagtactg
    28141 gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca tttagattta
    28201 ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg aaaatcgaca
    28261 ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc tatcggtgaa
    28321 acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga taaacggatg
    28381 attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga
    28441 gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac actgataggc
    28501 aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat tcagcaacct
    28561 aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt gacgattttg
    28621 gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga ccgttatttc
    28681 ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca agaactttat
    28741 tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc tgctgaaaaa
    28801 gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc ttacatggaa
    28861 caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa aatctcattg
    28921 ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga attgcaaaac
    28981 gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga atacttgagt
    29041 gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa
    29101 aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca ttaagcttat
    29161 tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca acgaaacgag
    29221 taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat acgaatatcc
    29281 gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa cgctacatca
    29341 aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt ttgacagatc
    29401 ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg
    29461 atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat caatggcgtc
    29521 actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc gaagcaatca
    29581 aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa gatgttccta
    29641 gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag ggacggctaa
    29701 gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc aacattgcaa
    29761 gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt ggcgaatttg
    29821 ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg aaagaacaat
    29881 tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag cagtcagtat
    29941 ttgattttga gatggaagat atccaattag aaggatatga cccacatccg gcgatcaaag
    30001 ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat ttgggcacag
    30061 gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg
    30121 aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa aacattcgaa
    30181 gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag agattcttcc
    30241 tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa
    30301 gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga atttctgcca
    30361 ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga tgcttatttt
    30421 ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga aatggatgaa
    30481 aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc
    30541 gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta tttagagctg
    30601 tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa gtacataaaa
    30661 cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat agaattcaag
    30721 ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa aataacacca
    30781 gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca ccccataaaa
    30841 atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt caacgcttta
    30901 tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca acctagccat
    30961 cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag aaaaatcgaa
    31021 ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga atgaaacaaa
    31081 gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga gacaatatgg
    31141 agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc gatactcaat
    31201 ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc atacgttcga
    31261 ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa tgaaaaatca
    31321 ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac atgaataagg
    31381 catcttgtta aagaaagaga gaga
//
LOCUS       DADVXN010000302        20985 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.047, whole genome shotgun sequence.
ACCESSION   DADVXN010000302 DADVXN010000000
VERSION     DADVXN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.047"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(134..577)
                     /locus_tag="KB942_003022"
     CDS             complement(134..577)
                     /locus_tag="KB942_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6853359.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="KB942_003023"
     CDS             complement(795..2135)
                     /locus_tag="KB942_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6853360.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="KB942_003024"
     CDS             2404..3300
                     /locus_tag="KB942_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6853361.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="KB942_003025"
     CDS             complement(3450..4391)
                     /locus_tag="KB942_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6853362.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="KB942_003026"
     CDS             4517..5638
                     /locus_tag="KB942_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6853363.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB942_003027"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB942_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6853364.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="KB942_003028"
     CDS             7426..7992
                     /locus_tag="KB942_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6853365.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="KB942_003029"
     CDS             8203..9264
                     /locus_tag="KB942_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6853366.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="KB942_003030"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="KB942_003030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB6853367.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="KB942_003031"
     CDS             10177..12159
                     /locus_tag="KB942_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6853368.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="KB942_003032"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="KB942_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB6853369.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="KB942_003033"
     CDS             12881..14305
                     /locus_tag="KB942_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6853370.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="KB942_003034"
     CDS             complement(14486..15259)
                     /locus_tag="KB942_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6853371.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="KB942_003035"
     CDS             complement(15256..16161)
                     /locus_tag="KB942_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6853372.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="KB942_003036"
     CDS             complement(16315..16635)
                     /locus_tag="KB942_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBB6853373.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="KB942_003037"
     CDS             17109..17795
                     /locus_tag="KB942_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBB6853374.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="KB942_003038"
     CDS             17797..18741
                     /locus_tag="KB942_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBB6853375.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="KB942_003039"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="KB942_003039"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBB6853376.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="KB942_003040"
     CDS             19663..20940
                     /locus_tag="KB942_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853377.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DADVXN010000303        13213 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.081, whole genome shotgun sequence.
ACCESSION   DADVXN010000303 DADVXN010000000
VERSION     DADVXN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.081"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(204..2162)
                     /locus_tag="KB942_003041"
     CDS             complement(204..2162)
                     /locus_tag="KB942_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6853378.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="KB942_003042"
     CDS             complement(2159..3031)
                     /locus_tag="KB942_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6853379.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="KB942_003043"
     CDS             complement(3373..4245)
                     /locus_tag="KB942_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6853380.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="KB942_003044"
     CDS             4373..4942
                     /locus_tag="KB942_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6853381.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="KB942_003045"
     CDS             5009..5746
                     /locus_tag="KB942_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6853382.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="KB942_003046"
     CDS             5977..7245
                     /locus_tag="KB942_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6853383.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="KB942_003047"
     CDS             7732..8196
                     /locus_tag="KB942_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6853384.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="KB942_003048"
     CDS             8180..8809
                     /locus_tag="KB942_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6853385.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB942_003049"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB942_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6853386.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="KB942_003050"
     CDS             10688..11134
                     /locus_tag="KB942_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6853387.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="KB942_003051"
     CDS             complement(11333..11848)
                     /locus_tag="KB942_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6853388.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="KB942_003052"
     CDS             11965..13167
                     /locus_tag="KB942_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6853389.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DADVXN010000304         7943 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.113, whole genome shotgun sequence.
ACCESSION   DADVXN010000304 DADVXN010000000
VERSION     DADVXN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.113"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            128..1564
                     /locus_tag="KB942_003053"
     CDS             128..1564
                     /locus_tag="KB942_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6853390.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="KB942_003054"
     CDS             1753..4185
                     /locus_tag="KB942_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB6853391.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="KB942_003055"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="KB942_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6853392.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5675..6778)
                     /locus_tag="KB942_003056"
     CDS             complement(5675..6778)
                     /locus_tag="KB942_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6853393.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="KB942_003057"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="KB942_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6853394.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DADVXN010000305         4702 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.156, whole genome shotgun sequence.
ACCESSION   DADVXN010000305 DADVXN010000000
VERSION     DADVXN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.156"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            194..1000
                     /locus_tag="KB942_003058"
     CDS             194..1000
                     /locus_tag="KB942_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6853395.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="KB942_003059"
     CDS             1018..1725
                     /locus_tag="KB942_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6853396.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="KB942_003060"
     CDS             1722..2693
                     /locus_tag="KB942_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6853397.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="KB942_003061"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="KB942_003061"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6853398.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="KB942_003062"
     CDS             3720..4328
                     /locus_tag="KB942_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6853399.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="KB942_003063"
     CDS             complement(4394..4567)
                     /locus_tag="KB942_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853400.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DADVXN010000306         3932 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.171, whole genome shotgun sequence.
ACCESSION   DADVXN010000306 DADVXN010000000
VERSION     DADVXN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.171"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            complement(202..572)
                     /locus_tag="KB942_003064"
                     /pseudo
     CDS             complement(202..572)
                     /locus_tag="KB942_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            717..902
                     /locus_tag="KB942_003065"
     CDS             717..902
                     /locus_tag="KB942_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6853401.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1060..1227
                     /locus_tag="KB942_003066"
     CDS             1060..1227
                     /locus_tag="KB942_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6853402.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1845..2504
                     /gene="vanR"
                     /locus_tag="KB942_003067"
     CDS             1845..2504
                     /gene="vanR"
                     /locus_tag="KB942_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6853403.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2507..3868
                     /gene="vanS"
                     /locus_tag="KB942_003068"
     CDS             2507..3868
                     /gene="vanS"
                     /locus_tag="KB942_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6853404.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         1129 a          811 c          978 g         1014 t
ORIGIN      
        1 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
       61 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
      121 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
      181 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
      241 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
      301 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
      361 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
      421 aatggcccgt cgttttcgat gttggcaaga tagcgccagt caatatttat tttctctgcc
      481 attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt tcacatccgc gccgaaagtt
      541 tcaaagccgg gacaatcttc aatattcgcc atatcgcacc tcccacttac attgtatatt
      601 tcatactctg cccgtggaat gttggtatat gcacctttct taaagtttat atttcacatt
      661 ttgtttcttt tattcttcga gcaaaagaat tataatgtac cctgtggagg tgtactatgg
      721 actacatgac gttgaaagag gcaggcgaga aatggggtgt gcccccacga tggataaact
      781 actattgtgc tgcgggacgc atccccggcg ctgtaaagat ggcgacgatc tggctcattc
      841 ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa aggaaaggcg gttactgaat
      901 agtataatga aaccggcgca ttttggatat ggttttgaag agagagaaag catggtattt
      961 tgtcacggaa aaactataag atgatagatg gcaggctgct ccagaccgat aaaaagtatt
     1021 ctcatctgaa acagaaagaa aagatttaca catggatgta tgaagaagcg aaactttatc
     1081 atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag ctgctgctgt atcagccgta
     1141 tatggtagaa ttgaaaaggc cggcatctgg attctttatg gtgaagtctc cagacattgg
     1201 ataactgctg gtctaatgcg cctttgagta tggggtgatt gattttaata ccggggaggt
     1261 acggcttttt gaggaaaata ttctgggatt tacatttggc gattattaca tttcctgtaa
     1321 ctgcaaggac acggatgatg aagatatgag gaatatagac ttgctgattt tctactgtct
     1381 tgaacggtat taggactgaa tctgctgcgc ggagtgcggc aagagcgacc ggagggagta
     1441 cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt gcattcgcaa aaaagaggcc
     1501 gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg
     1561 caagtggtgg atcagaatat ccgtaaagga tattctgatc cattgcttgc cagtattatc
     1621 gggatgtttt gacaaccgct gtaatcgaaa catcccaaac attaagaagg tgttaattat
     1681 tattatcttg atttaattcg tttaaaccaa taatatagat acatacacca tgaaaagaag
     1741 cgagtatccc aatgacggat gattaaaatc cgaaaagaaa tcgctttgaa actacaggga
     1801 aactacagac tgttatgtta tcttcttaaa atggagggat ttttatgtcg atacgaattc
     1861 tacttgtcga ggatgatgat catatctgta atacagtaag ggcgtttctg gctgaggcag
     1921 gatatcaggt ggatgcctgc acagatggaa atgaggcata caccaagttt tacgaaaaca
     1981 cttatcaact ggttattctt gatattatgc tgcccggtat gaacgggcat gaacttttgc
     2041 gtgaatttcg tgcgaaaaat gatactccca ttctgatgat gacagccctg tcggatgacg
     2101 aaaaccaaat ccgggcgttt gatgcagagg cagacgacta tgtaacaaag ccattcaaga
     2161 tgcagatttt actaaagcgg gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg
     2221 aaatccgtgt cggcaggctg acacttctgc cggaggattt cacggtactt tgtgacggta
     2281 cagagctgcc cctgacacga aaagaatttg aaatcctttt gctgctggtg cagaacaaag
     2341 gcagaacctt aacgcatgaa atcattttgt cacgtatatg gggatatgac tttgagggtg
     2401 atggcagcac agtccacact catatcaaaa atttgcgggc caagctgccg gaaaatatca
     2461 tcaaaaccat tcgcggtgta ggataccgat tggaggaatc ataataatgg aaagaaaagg
     2521 gattttcatt aaggtttttt cctatacgat cattgtcctg ctactgcttg tcggtgtaac
     2581 agcaacactg tttgcacagc aatttgtgtc ttatttcaga gtgatggaat tacagcaaac
     2641 agtaaaatcc tatcagccat tggtggaact gattcagaat agcgataggc ttgatattca
     2701 agaggtggca gggctgtttc actacaataa ccaatccttt gagttttata ttgaagataa
     2761 agagggaagc gtactctatg ccacaccaaa tgccaataca tcaaatagtt ttagacccaa
     2821 ctttctttat gtggtacata gagatgataa tatttcgatt gttgctcaaa gcaaggcagg
     2881 tgtgggattg ctttatcaag ggctgacaat tcggggaatt gttatgattg cgataatggt
     2941 tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc
     3001 cttagcggac agtgcgaata aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg
     3061 aaaggatgag cttggcgcac tggctcatga tatgcattcc atgtatgtca ggctgaaaga
     3121 aaccatcgca aggctggagg atgaaatcgc aagggaacat gagttggagg aaacacagcg
     3181 atatttcttt gcggcagctt ctcatgagct aaaaacgccc atcgcggcta caagcgttct
     3241 gttggaggga atgcttgaaa atatcggtga ctacaaagat cattctaagt atctgcgcga
     3301 atgcatcaaa atgatggata ggcagggcaa aatcatttcc gaaatactgg agcttgtcag
     3361 cctgaatgat gggagaatcg tacccatagc tgaaccgttg gacatagggc gcacggttgc
     3421 cgagttgctg cccgattttc aaaccttggc agaggcaaac aaccagcggt tcgtcacaga
     3481 tattccagcc gggcaaattg tcctgtccga tccgaggctg ctccaaaagg cactatccaa
     3541 tgtcatattg aatgcagttc agaacacgcc gcagggaggc gaggtacgga tatggagtga
     3601 gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg ggcgttcaca ttgatgatac
     3661 tgcgcttcca aggctgttca ccccattcta tcgcattgat caggcgcgaa gcagaaaaag
     3721 tgggcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct
     3781 ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct
     3841 ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt tgggctcttt
     3901 gtcaaatagg actgatagag tgagataaga ac
//
LOCUS       DADVXN010000307         1838 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.220, whole genome shotgun sequence.
ACCESSION   DADVXN010000307 DADVXN010000000
VERSION     DADVXN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.220"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            322..1014
                     /locus_tag="KB942_003069"
     CDS             322..1014
                     /locus_tag="KB942_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6853405.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="KB942_003070"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="KB942_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6853406.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          759 a          229 c          327 g          523 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaa
//
LOCUS       DADVXN010000308         1374 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301553-19
            SAMEA8426450-rid11941483.guided.235, whole genome shotgun sequence.
ACCESSION   DADVXN010000308 DADVXN010000000
VERSION     DADVXN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426450
            Sequence Read Archive: ERR5555415
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005864.1
            Genome Coverage       :: 269x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 09:39:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 27 of 64
            Pseudo Genes (incomplete)         :: 28 of 64
            Pseudo Genes (internal stop)      :: 16 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426450-rid11941483.guided.235"
                     /isolate="301553-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-04"
     gene            110..193
                     /locus_tag="KB942_003071"
     CDS             110..193
                     /locus_tag="KB942_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6853407.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="KB942_003072"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="KB942_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6853408.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          506 a          217 c          222 g          429 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agct
//
